Phb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phb
Entrez Gene ID	25344
Full Name	prohibitin
General protein information	Preferred Names prohibitin Names prohibitin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	inhibits entry into S phase of the mitotic cell cycle; involved in negative regulation of cell proliferation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031851.2	NP_114039.1	prohibitin

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	phb	NP_958454.1
Kluyveromyces lactis	KLLA0D16302g	XP_453779.1
Oryza sativa (rice)	Os02g0580500	NP_001047236.1
Pan troglodytes (chimpanzee)	PHB	NP_001233332.1
Rattus norvegicus (Norway rat)	Phb	NP_114039.1
Gallus gallus (chicken)	PHB	NP_001171206.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009323	XP_309992.2
Magnaporthe oryzae (rice blast fungus)	MGG_06004	XP_003711850.1
Xenopus tropicalis (tropical clawed frog)	phb	NP_989038.1
Homo sapiens (human)	PHB	NP_001268425.1
	PHB	XP_001093453.1
Saccharomyces cerevisiae (baker's yeast)	PHB1	NP_011648.3
Oryza sativa (rice)	Os04g0462900	NP_001053006.2
Eremothecium gossypii	AGOS_AFR313C	NP_985860.1
Schizosaccharomyces pombe (fission yeast)	phb1	NP_594713.1
Neurospora crassa	NCU08946	XP_960813.2
Canis lupus familiaris (dog)	PHB	NP_001103649.1
Mus musculus (house mouse)	Phb	NP_032857.1
Drosophila melanogaster (fruit fly)	l(2)37Cc	NP_724165.1
Bos taurus (cattle)	PHB	NP_001029744.1
Caenorhabditis elegans (roundworm)	phb-1	NP_490929.1
Arabidopsis thaliana (thale cress)	PHB4	NP_189364.1
Arabidopsis thaliana (thale cress)	PHB3	NP_198893.1

Gene Ontology
Bibliography

Related articles in PubMed

Prohibitin confers cytoprotection against ISO-induced hypertrophy in H9c2 cells via attenuation of oxidative stress and modulation of Akt/Gsk-3? signaling.
Chowdhury D, Kumar D, Bhadra U, Devi TA, Bhadra MP
Molecular and cellular biochemistry425(1-2)155-168(2017 Jan)

Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation.
Chowdhury I, Thomas K, Zeleznik A, Thompson WE
Journal of molecular endocrinology56(4)325-36(2016 05)

Prohibitin overexpression improves myocardial function in diabetic cardiomyopathy.
Dong WQ, Chao M, Lu QH, Chai WL, Zhang W, Chen XY, Liang ES, Wang LB, Tian HL, Chen YG, Zhang MX
Oncotarget7(1)66-80(2016 Jan)

F-box protein 7 mutations promote protein aggregation in mitochondria and inhibit mitophagy.
Zhou ZD, Xie SP, Sathiyamoorthy S, Saw WT, Sing TY, Ng SH, Chua HP, Tang AM, Shaffra F, Li Z, Wang H, Ho PG, Lai MK, Angeles DC, Lim TM, Tan EK
Human molecular genetics24(22)6314-30(2015 Nov)

Effect of estrogen on expression of prohibitin in white adipose tissue and liver of diet-induced obese rats.
Choi M, Chaudhari HN, Ji YR, Ryoo ZY, Kim SW, Yun JW
Molecular and cellular biochemistry407(1-2)181-96(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that PHB confers cytoprotection against oxidative stress in ISO-induced hypertrophy and this process is associated with modulation of Akt/Gsk-3beta signaling mechanisms as evident from our PHB overexpression and knockdown experiments.
Title: Prohibitin confers cytoprotection against ISO-induced hypertrophy in H9c2 cells via attenuation of oxidative stress and modulation of Akt/Gsk-3? signaling.

gonadotroph-in-dependent steroidogenesis in undifferentiated GCs are influenced not only by the expression of the key steroidogenic enzymes, but also by the 30-kDa PHB/pPHB, which acts as an obligatory mitochondrial protein that regulates mitochondrial function and, thus plays a central role as a key modulator of GC differentiation.
Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation.

Data suggest that prohibitin (PHB) may be a new therapeutic target for diabetic cardiomyopathy (DCM).
Title: Prohibitin overexpression improves myocardial function in diabetic cardiomyopathy.

Study shows that PHB is highly expressed in white adipose tissue and liver which expression is regulated by estrogen.
Title: Effect of estrogen on expression of prohibitin in white adipose tissue and liver of diet-induced obese rats.

PHB1 plays an important role in the development of SCI and might provide a therapeutic target to promote recovery from SCI.
Title: Prohibitin 1 gene delivery promotes functional recovery in rats with spinal cord injury.

The following Phb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01176	NM_031851.2 Latest version!	Rattus norvegicus prohibitin (Phb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phb ( NM_031851.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114039.1 Rattus norvegicus prohibitin (Phb), mRNA.

Price & Availability↑

CloneID	ORa01176
Clone ID Related Accession (Same CDS sequence)	NM_031851.2
Accession Version	NM_031851.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	prohibitin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC072518.1. On Apr 20, 2006 this sequence version replaced NM_031851.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072518.1, FQ229278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCA AAGTGTTTGA GTCCATCGGA AAGTTCGGCC TGGCCTTAGC AGTTGCAGGA 
GGCGTGGTGA ACTCTGCTCT ATATAACGTG GATGCCGGAC ACAGAGCTGT CATCTTCGAC 
CGATTCCGTG GCGTGCAGGA CATCGTGGTA GGGGAAGGGA CTCACTTCCT CATCCCCTGG 
GTACAGAAGC CAATCATCTT TGACTGCCGC TCTCGACCAC GTAATGTGCC GGTCATCACC 
GGCAGCAAAG ACTTGCAGAA TGTCAACATC ACACTACGTA TCCTCTTCCG GCCGGTGGCC 
AGCCAGCTTC CTCGTATCTA CACCAGCATT GGCGAGGACT ATGATGAGCG GGTGCTGCCA 
TCTATCACCA CAGAGATCCT CAAGTCGGTG GTGGCTCGAT TCGATGCTGG AGAATTGATT 
ACCCAGCGAG AGCTGGTCTC CAGGCAGGTG AGTGATGACC TCACAGAGCG AGCAGCAACA 
TTCGGGCTCA TCCTGGATGA CGTGTCCCTG ACACATCTGA CCTTCGGGAA GGAGTTCACA 
GAGGCGGTGG AAGCCAAACA GGTGGCTCAG CAGGAAGCAG AGAGAGCCAG ATTTGTGGTG 
GAAAAGGCTG AGCAGCAGAA GAAGGCGGCC ATCATCTCTG CTGAGGGTGA CTCCAAAGCG 
GCTGAGCTGA TCGCCAACTC ACTGGCCACC GCCGGGGATG GCCTGATCGA GCTGCGAAAG 
CTGGAAGCTG CTGAGGACAT TGCTTATCAG CTCTCCCGCT CTCGGAACAT CACCTACCTG 
CCAGCAGGGC AGTCCGTGCT CCTCCAGCTC CCCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114039.1
CDS	59..877
Translation	MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIYTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ

Target ORF information:

RefSeq Version	NM_031851.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prohibitin (Phb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031851.2

ORF Insert Sequence:

ATGGCTGCCA AAGTGTTTGA GTCCATCGGA AAGTTCGGCC TGGCCTTAGC AGTTGCAGGA 
GGCGTGGTGA ACTCTGCTCT ATATAACGTG GATGCCGGAC ACAGAGCTGT CATCTTCGAC 
CGATTCCGTG GCGTGCAGGA CATCGTGGTA GGGGAAGGGA CTCACTTCCT CATCCCCTGG 
GTACAGAAGC CAATCATCTT TGACTGCCGC TCTCGACCAC GTAATGTGCC GGTCATCACC 
GGCAGCAAAG ACTTGCAGAA TGTCAACATC ACACTACGTA TCCTCTTCCG GCCGGTGGCC 
AGCCAGCTTC CTCGTATCTA CACCAGCATT GGCGAGGACT ATGATGAGCG GGTGCTGCCA 
TCTATCACCA CAGAGATCCT CAAGTCGGTG GTGGCTCGAT TCGATGCTGG AGAATTGATT 
ACCCAGCGAG AGCTGGTCTC CAGGCAGGTG AGTGATGACC TCACAGAGCG AGCAGCAACA 
TTCGGGCTCA TCCTGGATGA CGTGTCCCTG ACACATCTGA CCTTCGGGAA GGAGTTCACA 
GAGGCGGTGG AAGCCAAACA GGTGGCTCAG CAGGAAGCAG AGAGAGCCAG ATTTGTGGTG 
GAAAAGGCTG AGCAGCAGAA GAAGGCGGCC ATCATCTCTG CTGAGGGTGA CTCCAAAGCG 
GCTGAGCTGA TCGCCAACTC ACTGGCCACC GCCGGGGATG GCCTGATCGA GCTGCGAAAG 
CTGGAAGCTG CTGAGGACAT TGCTTATCAG CTCTCCCGCT CTCGGAACAT CACCTACCTG 
CCAGCAGGGC AGTCCGTGCT CCTCCAGCTC CCCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prohibitin confers cytoprotection against ISO-induced hypertrophy in H9c2 cells via attenuation of oxidative stress and modulation of Akt/Gsk-3? signaling.
Molecular and cellular biochemistry425(1-2)155-168(2017 Jan)
Chowdhury D,Kumar D,Bhadra U,Devi TA,Bhadra MP

Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation.
Journal of molecular endocrinology56(4)325-36(2016 05)
Chowdhury I,Thomas K,Zeleznik A,Thompson WE

Prohibitin overexpression improves myocardial function in diabetic cardiomyopathy.
Oncotarget7(1)66-80(2016 Jan)
Dong WQ,Chao M,Lu QH,Chai WL,Zhang W,Chen XY,Liang ES,Wang LB,Tian HL,Chen YG,Zhang MX

F-box protein 7 mutations promote protein aggregation in mitochondria and inhibit mitophagy.
Human molecular genetics24(22)6314-30(2015 Nov)
Zhou ZD,Xie SP,Sathiyamoorthy S,Saw WT,Sing TY,Ng SH,Chua HP,Tang AM,Shaffra F,Li Z,Wang H,Ho PG,Lai MK,Angeles DC,Lim TM,Tan EK

Effect of estrogen on expression of prohibitin in white adipose tissue and liver of diet-induced obese rats.
Molecular and cellular biochemistry407(1-2)181-96(2015 Sep)
Choi M,Chaudhari HN,Ji YR,Ryoo ZY,Kim SW,Yun JW

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Phb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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