Lipe cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lipe
Entrez Gene ID	25330
Full Name	lipase E, hormone sensitive type
Synonyms	HSL
General protein information	Preferred Names lipase E, hormone sensitive type Names hormone-sensitive lipase hormone - sensitive lipase testicular isoform lipase, hormone sensitive
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	enzyme involved in hydrolysis of stored triglycerides and fatty acids; important for regulation of energy homeostasis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006228391.2	XP_006228453.1	hormone-sensitive lipase isoform X1
XM_008758895.2	XP_008757117.1	hormone-sensitive lipase isoform X2
XM_006228393.2	XP_006228455.1	hormone-sensitive lipase isoform X3
XM_006228394.3	XP_006228456.1	hormone-sensitive lipase isoform X4
NM_012859.1	NP_036991.1	hormone-sensitive lipase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	lipeb	XP_686139.6
Pan troglodytes (chimpanzee)	LIPE	XP_003316431.1
Xenopus tropicalis (tropical clawed frog)	lipe	NP_001231911.1
Bos taurus (cattle)	LIPE	NP_001073689.1
Canis lupus familiaris (dog)	LIPE	XP_533655.4
Mus musculus (house mouse)	Lipe	NP_034849.2
Rattus norvegicus (Norway rat)	Lipe	NP_036991.1
Homo sapiens (human)	LIPE	NP_005348.2

Gene Ontology
Bibliography

Related articles in PubMed

Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes in rat primary white adipocytes.
Ogasawara J, Izawa T, Sakurai T, Shirato K, Ishibashi Y, Ohira Y, Ishida H, Ohno H, Kizaki T
Biochemical and biophysical research communications464(1)348-53(2015 Aug)

Progesterone-induced down-regulation of hormone sensitive lipase (Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis.
Stelmanska E, Szrok S, Swierczynski J
The Journal of steroid biochemistry and molecular biology14731-9(2015 Mar)

Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation.
Macpherson RE, Vandenboom R, Roy BD, Peters SJ
Physiological reports1(4)e00084(2013 Sep)

Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.
Ogasawara J, Nomura S, Rahman N, Sakurai T, Kizaki T, Izawa T, Ishida H, Haga S, Ohno H
Biochemical and biophysical research communications400(1)134-9(2010 Sep)

Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area.
Krintel C, M?rgelin M, Logan DT, Holm C
The FEBS journal276(17)4752-62(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes, HSL and ATGL, in rat primary white adipocytes.
Title: Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes in rat primary white adipocytes.

The findings suggest that progesterone, acting in a progesterone receptor-dependent manner, down-regulates Lipe gene expression but exerts no effect on Pnpla2 gene expression in the inguinal white adipose tissue of females.
Title: Progesterone-induced down-regulation of hormone sensitive lipase (Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis.

These findings suggest that acute exercise enhances lipolysis up to at least 3h after exercise in a manner dependent on modification of HSL and its association with and alteration in scaffold protein.
Title: Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.

PKA phosphorylation induces a conformational change that increases the exposed hydrophobic surface and thereby facilitates binding of HSL to the lipid substrate.
Title: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area.

The retinyl ester hydrolase activity of rat HSL was found to be higher than that against the hitherto best known HSL substrate, i.e., diacylglycerols.
Title: Hormone-sensitive lipase (HSL) is also a retinyl ester hydrolase: evidence from mice lacking HSL.

The following Lipe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lipe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa22391	XM_006228391.2 Latest version!	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa22393	XM_006228393.2 Latest version!	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa22394	XM_006228394.3 Latest version!	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43072	XM_008758895.2 Latest version!	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa01140	NM_012859.1 Latest version!	Rattus norvegicus lipase E, hormone sensitive type (Lipe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lipe ( XM_006228391.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228453.1 Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22391
Clone ID Related Accession (Same CDS sequence)	XM_006228391.2
Accession Version	XM_006228391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hormone-sensitive lipase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006228391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG CCGTGGAATC GGCGCCCGTG GTGGCCCAGG CCTCCAAGCA GGCCAAAGAA 
GGATCGAAGA ACCGCAGTCG CAGGCGGTGG CGAAAAGGCA AGGTCAAAGC CTCGGCATTC 
TCACACAGCA TGGATTTACG CACAATGACA CAGTCGCTGG TGGCGCTCGC AGAAGACAAC 
ATGGCCTTCT TCTCAAGCCA GGGCCCAGGA GAGACAGCAC GGCGGCTGTC CAACGTCTTT 
GCAGGTGTTC GGGAACAGGC ACTGGGGCTA GAACCAACTC TAGGTCAGCT CTTGGGTGTG 
GCACACCATT TCGACCTGGA CACTGAGACA CCAGCCAACG GATACCGTAG CTTGGTGCAC 
ACAGCTCGTT GCTGCCTGGC ACACCTACTA CACAAATCCC GCTATGTGGC CTCTAACCGC 
AGAAGTATCT TCTTCCGTGC CAGCCACAAC CTAGCAGAAC TGGAGGCCTA CCTGGCTGCC 
CTCACCCAGC TCCGTGCTCT AGCCTACTAC GCCCAGCGCC TGCTGACCAT CAACCGACCA 
GGAGTGCTCT TCTTTGAGGG CGATGAGGGA CTCAGCGCTG ACTTCCTACA GGACTATGTC 
ACGCTACATA AAGGCTGCTT TTACGGTCGC TGCCTGGGCT TCCAGTTCAC ACCTGCCATC 
CGGCCGTTCC TGCAGACTCT CTCCATCGGG CTGGTGTCCT TCGGGGAACA CTACAAACGC 
AACGAGACGG GCCTCAGTGT GACTGCAAGT TCCCTCTTTA CGGGTGGCCG ATTCGCCATA 
GACCCAGAGT TGCGTGGGGC TGAATTTGAG CGCATCATAC AGAACCTGGA CGTGCACTTC 
TGGAAAGCCT TCTGGAATAT CACGGAGATT GAGGTGCTAT CGTCTCTGGC CAACATGGCA 
TCAACCACCG TGAGGGTAAG CCGCCTGCTT AGCTTGCCGC CTGAGGCCTT TGAGATGCCA 
CTAACCTCTG ACCCCAAGCT CACAGTTACC ATCTCACCTC CCTTGGCACA CACGGGACCA 
GGCCCTGTGC TAGCCAGGCT CATCTCCTAT GACCTGCGGG AAGGGCAGGA CAGCAAGATG 
CTCAACAGCC TGGCAAAATC TGAGGGCCCA CGCCTGGAGC TGCGCCCACG GCCTCAACAA 
GCCCCCCGCT CACGAGCCCT GGTTGTCCAC ATTCACGGTG GTGGCTTTGT GGCACAGACC 
TCCAAATCCC ATGAGCCCTA CCTCAAGAAC TGGGCCCAGG AGCTAGGCGT CCCCATCATC 
TCCATCGACT ACTCCCTGGC CCCTGAGGCC CCCTTCCCCC GAGCACTGGA GGAGTGTTTT 
TTTGCCTACT GCTGGGCTGT CAAGCACTGT GAACTGCTTG GTTCAACCGG AGAGCGGATA 
TGCCTTGCGG GGGACAGCGC AGGTGGGAAC CTCTGCATCA CCGTGTCCCT TCGGGCAGCA 
GCCTATGGGG TGAGGGTGCC AGATGGCATC ATGGCAGCCT ACCCAGTTAC CACCCTGCAG 
TCCTCTGCTT CTCCCTCTCG TCTGCTGAGC CTCATGGATC CTCTTCTACC ACTGAGCGTA 
CTCTCCAAGT GTGTGAGCGC CTATTCAGGG ACAGAGACGG AGGACCATTT TGACTCAGAC 
CAGAAGGCAT TGGGCGTGAT GGGGCTGGTG CAGAGAGACA CGTCCCTGTT CCTCAGAGAC 
CTCCGCCTGG GCGCCTCCTC ATGGCTCAAC TCCTTCCTGG AGTTAAGTGG GCGCAAGCCC 
CATAAGACCC CATTGCCCGC AACAGAGACA CTGCGCCCCA CGGATTCTGG GCGACTTACG 
GAGTCTATGC GCAGGAGTGT GTCTGAGGCA GCCCTGGCCC AGCCTGAGGG CTTGCTGGGC 
ACAGATTCCT TGAAGAAGCT AACAATAAAG GACTTGAGCT TTAAGGGCAA CTCAGAGCCA 
TCAGACAGCC CCGAGATGTC ACAGTCAATG GAGACACTTG GCCCCTCCAC ACCCTCGGAT 
GTCAACTTTT TTCTGCGATC CGGGAATTCC CAGGAAGAGG CTGAAACCAG AGATGATATA 
AGCCCTATGG ACGGAATCCC CCGCGTGCGC GCTGCCTTCC CTGATGGTTT CCACCCACGG 
CGCTCAAGCC AAGGTGTCCT CCACATGCCC CTCTACTCGT CACCCATAGT CAAGAACCCC 
TTCATGTCTC CTCTGCTGGC CCCTGACGTC ATGCTGAAGA CCCTGCCGCC CGTGCACCTT 
GTGGCGTGCG CTCTGGACCC CATGCTGGAT GACTCGGTCA TGTTCGCGCG GCGACTGAAG 
GACCTGGGCC AGCCCGTGAC GCTGAAAGTG GTAGAGGACC TGCCGCATGG TTTCCTGAGC 
TTAGCAGCCC TGTGTCGTGA GACCCGGCAG GCCGCGGAGT TGTGCGTGCA GCGCATCCGG 
CTGATCCTCA CCCCCCCTGC TGCACCGCTG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228453.1
CDS	98..2533
Translation	MEPAVESAPVVAQASKQAKEGSKNRSRRRWRKGKVKASAFSHSMDLRTMTQSLVALAEDNMAFFSSQGPGETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQRLLTINRPGVLFFEGDEGLSADFLQDYVTLHKGCFYGRCLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLFTGGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTSDPKLTVTISPPLAHTGPGPVLARLISYDLREGQDSKMLNSLAKSEGPRLELRPRPQQAPRSRALVVHIHGGGFVAQTSKSHEPYLKNWAQELGVPIISIDYSLAPEAPFPRALEECFFAYCWAVKHCELLGSTGERICLAGDSAGGNLCITVSLRAAAYGVRVPDGIMAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTETEDHFDSDQKALGVMGLVQRDTSLFLRDLRLGASSWLNSFLELSGRKPHKTPLPATETLRPTDSGRLTESMRRSVSEAALAQPEGLLGTDSLKKLTIKDLSFKGNSEPSDSPEMSQSMETLGPSTPSDVNFFLRSGNSQEEAETRDDISPMDGIPRVRAAFPDGFHPRRSSQGVLHMPLYSSPIVKNPFMSPLLAPDVMLKTLPPVHLVACALDPMLDDSVMFARRLKDLGQPVTLKVVEDLPHGFLSLAALCRETRQAAELCVQRIRLILTPPAAPLT

Target ORF information:

RefSeq Version	XM_006228391.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228391.2

ORF Insert Sequence:

ATGGAGCCGG CCGTGGAATC GGCGCCCGTG GTGGCCCAGG CCTCCAAGCA GGCCAAAGAA 
GGATCGAAGA ACCGCAGTCG CAGGCGGTGG CGAAAAGGCA AGGTCAAAGC CTCGGCATTC 
TCACACAGCA TGGATTTACG CACAATGACA CAGTCGCTGG TGGCGCTCGC AGAAGACAAC 
ATGGCCTTCT TCTCAAGCCA GGGCCCAGGA GAGACAGCAC GGCGGCTGTC CAACGTCTTT 
GCAGGTGTTC GGGAACAGGC ACTGGGGCTA GAACCAACTC TAGGTCAGCT CTTGGGTGTG 
GCACACCATT TCGACCTGGA CACTGAGACA CCAGCCAACG GATACCGTAG CTTGGTGCAC 
ACAGCTCGTT GCTGCCTGGC ACACCTACTA CACAAATCCC GCTATGTGGC CTCTAACCGC 
AGAAGTATCT TCTTCCGTGC CAGCCACAAC CTAGCAGAAC TGGAGGCCTA CCTGGCTGCC 
CTCACCCAGC TCCGTGCTCT AGCCTACTAC GCCCAGCGCC TGCTGACCAT CAACCGACCA 
GGAGTGCTCT TCTTTGAGGG CGATGAGGGA CTCAGCGCTG ACTTCCTACA GGACTATGTC 
ACGCTACATA AAGGCTGCTT TTACGGTCGC TGCCTGGGCT TCCAGTTCAC ACCTGCCATC 
CGGCCGTTCC TGCAGACTCT CTCCATCGGG CTGGTGTCCT TCGGGGAACA CTACAAACGC 
AACGAGACGG GCCTCAGTGT GACTGCAAGT TCCCTCTTTA CGGGTGGCCG ATTCGCCATA 
GACCCAGAGT TGCGTGGGGC TGAATTTGAG CGCATCATAC AGAACCTGGA CGTGCACTTC 
TGGAAAGCCT TCTGGAATAT CACGGAGATT GAGGTGCTAT CGTCTCTGGC CAACATGGCA 
TCAACCACCG TGAGGGTAAG CCGCCTGCTT AGCTTGCCGC CTGAGGCCTT TGAGATGCCA 
CTAACCTCTG ACCCCAAGCT CACAGTTACC ATCTCACCTC CCTTGGCACA CACGGGACCA 
GGCCCTGTGC TAGCCAGGCT CATCTCCTAT GACCTGCGGG AAGGGCAGGA CAGCAAGATG 
CTCAACAGCC TGGCAAAATC TGAGGGCCCA CGCCTGGAGC TGCGCCCACG GCCTCAACAA 
GCCCCCCGCT CACGAGCCCT GGTTGTCCAC ATTCACGGTG GTGGCTTTGT GGCACAGACC 
TCCAAATCCC ATGAGCCCTA CCTCAAGAAC TGGGCCCAGG AGCTAGGCGT CCCCATCATC 
TCCATCGACT ACTCCCTGGC CCCTGAGGCC CCCTTCCCCC GAGCACTGGA GGAGTGTTTT 
TTTGCCTACT GCTGGGCTGT CAAGCACTGT GAACTGCTTG GTTCAACCGG AGAGCGGATA 
TGCCTTGCGG GGGACAGCGC AGGTGGGAAC CTCTGCATCA CCGTGTCCCT TCGGGCAGCA 
GCCTATGGGG TGAGGGTGCC AGATGGCATC ATGGCAGCCT ACCCAGTTAC CACCCTGCAG 
TCCTCTGCTT CTCCCTCTCG TCTGCTGAGC CTCATGGATC CTCTTCTACC ACTGAGCGTA 
CTCTCCAAGT GTGTGAGCGC CTATTCAGGG ACAGAGACGG AGGACCATTT TGACTCAGAC 
CAGAAGGCAT TGGGCGTGAT GGGGCTGGTG CAGAGAGACA CGTCCCTGTT CCTCAGAGAC 
CTCCGCCTGG GCGCCTCCTC ATGGCTCAAC TCCTTCCTGG AGTTAAGTGG GCGCAAGCCC 
CATAAGACCC CATTGCCCGC AACAGAGACA CTGCGCCCCA CGGATTCTGG GCGACTTACG 
GAGTCTATGC GCAGGAGTGT GTCTGAGGCA GCCCTGGCCC AGCCTGAGGG CTTGCTGGGC 
ACAGATTCCT TGAAGAAGCT AACAATAAAG GACTTGAGCT TTAAGGGCAA CTCAGAGCCA 
TCAGACAGCC CCGAGATGTC ACAGTCAATG GAGACACTTG GCCCCTCCAC ACCCTCGGAT 
GTCAACTTTT TTCTGCGATC CGGGAATTCC CAGGAAGAGG CTGAAACCAG AGATGATATA 
AGCCCTATGG ACGGAATCCC CCGCGTGCGC GCTGCCTTCC CTGATGGTTT CCACCCACGG 
CGCTCAAGCC AAGGTGTCCT CCACATGCCC CTCTACTCGT CACCCATAGT CAAGAACCCC 
TTCATGTCTC CTCTGCTGGC CCCTGACGTC ATGCTGAAGA CCCTGCCGCC CGTGCACCTT 
GTGGCGTGCG CTCTGGACCC CATGCTGGAT GACTCGGTCA TGTTCGCGCG GCGACTGAAG 
GACCTGGGCC AGCCCGTGAC GCTGAAAGTG GTAGAGGACC TGCCGCATGG TTTCCTGAGC 
TTAGCAGCCC TGTGTCGTGA GACCCGGCAG GCCGCGGAGT TGTGCGTGCA GCGCATCCGG 
CTGATCCTCA CCCCCCCTGC TGCACCGCTG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lipe ( XM_006228393.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228455.1 Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa22393
Clone ID Related Accession (Same CDS sequence)	XM_006228393.2
Accession Version	XM_006228393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hormone-sensitive lipase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006228393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCG CCTCGGCATT CTCACACAGC ATGGATTTAC GCACAATGAC ACAGTCGCTG 
GTGGCGCTCG CAGAAGACAA CATGGCCTTC TTCTCAAGCC AGGGCCCAGG AGAGACAGCA 
CGGCGGCTGT CCAACGTCTT TGCAGGTGTT CGGGAACAGG CACTGGGGCT AGAACCAACT 
CTAGGTCAGC TCTTGGGTGT GGCACACCAT TTCGACCTGG ACACTGAGAC ACCAGCCAAC 
GGATACCGTA GCTTGGTGCA CACAGCTCGT TGCTGCCTGG CACACCTACT ACACAAATCC 
CGCTATGTGG CCTCTAACCG CAGAAGTATC TTCTTCCGTG CCAGCCACAA CCTAGCAGAA 
CTGGAGGCCT ACCTGGCTGC CCTCACCCAG CTCCGTGCTC TAGCCTACTA CGCCCAGCGC 
CTGCTGACCA TCAACCGACC AGGAGTGCTC TTCTTTGAGG GCGATGAGGG ACTCAGCGCT 
GACTTCCTAC AGGACTATGT CACGCTACAT AAAGGCTGCT TTTACGGTCG CTGCCTGGGC 
TTCCAGTTCA CACCTGCCAT CCGGCCGTTC CTGCAGACTC TCTCCATCGG GCTGGTGTCC 
TTCGGGGAAC ACTACAAACG CAACGAGACG GGCCTCAGTG TGACTGCAAG TTCCCTCTTT 
ACGGGTGGCC GATTCGCCAT AGACCCAGAG TTGCGTGGGG CTGAATTTGA GCGCATCATA 
CAGAACCTGG ACGTGCACTT CTGGAAAGCC TTCTGGAATA TCACGGAGAT TGAGGTGCTA 
TCGTCTCTGG CCAACATGGC ATCAACCACC GTGAGGGTAA GCCGCCTGCT TAGCTTGCCG 
CCTGAGGCCT TTGAGATGCC ACTAACCTCT GACCCCAAGC TCACAGTTAC CATCTCACCT 
CCCTTGGCAC ACACGGGACC AGGCCCTGTG CTAGCCAGGC TCATCTCCTA TGACCTGCGG 
GAAGGGCAGG ACAGCAAGAT GCTCAACAGC CTGGCAAAAT CTGAGGGCCC ACGCCTGGAG 
CTGCGCCCAC GGCCTCAACA AGCCCCCCGC TCACGAGCCC TGGTTGTCCA CATTCACGGT 
GGTGGCTTTG TGGCACAGAC CTCCAAATCC CATGAGCCCT ACCTCAAGAA CTGGGCCCAG 
GAGCTAGGCG TCCCCATCAT CTCCATCGAC TACTCCCTGG CCCCTGAGGC CCCCTTCCCC 
CGAGCACTGG AGGAGTGTTT TTTTGCCTAC TGCTGGGCTG TCAAGCACTG TGAACTGCTT 
GGTTCAACCG GAGAGCGGAT ATGCCTTGCG GGGGACAGCG CAGGTGGGAA CCTCTGCATC 
ACCGTGTCCC TTCGGGCAGC AGCCTATGGG GTGAGGGTGC CAGATGGCAT CATGGCAGCC 
TACCCAGTTA CCACCCTGCA GTCCTCTGCT TCTCCCTCTC GTCTGCTGAG CCTCATGGAT 
CCTCTTCTAC CACTGAGCGT ACTCTCCAAG TGTGTGAGCG CCTATTCAGG GACAGAGACG 
GAGGACCATT TTGACTCAGA CCAGAAGGCA TTGGGCGTGA TGGGGCTGGT GCAGAGAGAC 
ACGTCCCTGT TCCTCAGAGA CCTCCGCCTG GGCGCCTCCT CATGGCTCAA CTCCTTCCTG 
GAGTTAAGTG GGCGCAAGCC CCATAAGACC CCATTGCCCG CAACAGAGAC ACTGCGCCCC 
ACGGATTCTG GGCGACTTAC GGAGTCTATG CGCAGGAGTG TGTCTGAGGC AGCCCTGGCC 
CAGCCTGAGG GCTTGCTGGG CACAGATTCC TTGAAGAAGC TAACAATAAA GGACTTGAGC 
TTTAAGGGCA ACTCAGAGCC ATCAGACAGC CCCGAGATGT CACAGTCAAT GGAGACACTT 
GGCCCCTCCA CACCCTCGGA TGTCAACTTT TTTCTGCGAT CCGGGAATTC CCAGGAAGAG 
GCTGAAACCA GAGATGATAT AAGCCCTATG GACGGAATCC CCCGCGTGCG CGCTGCCTTC 
CCTGATGGTT TCCACCCACG GCGCTCAAGC CAAGGTGTCC TCCACATGCC CCTCTACTCG 
TCACCCATAG TCAAGAACCC CTTCATGTCT CCTCTGCTGG CCCCTGACGT CATGCTGAAG 
ACCCTGCCGC CCGTGCACCT TGTGGCGTGC GCTCTGGACC CCATGCTGGA TGACTCGGTC 
ATGTTCGCGC GGCGACTGAA GGACCTGGGC CAGCCCGTGA CGCTGAAAGT GGTAGAGGAC 
CTGCCGCATG GTTTCCTGAG CTTAGCAGCC CTGTGTCGTG AGACCCGGCA GGCCGCGGAG 
TTGTGCGTGC AGCGCATCCG GCTGATCCTC ACCCCCCCTG CTGCACCGCT GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228455.1
CDS	448..2784
Translation	MVLASAFSHSMDLRTMTQSLVALAEDNMAFFSSQGPGETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQRLLTINRPGVLFFEGDEGLSADFLQDYVTLHKGCFYGRCLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLFTGGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTSDPKLTVTISPPLAHTGPGPVLARLISYDLREGQDSKMLNSLAKSEGPRLELRPRPQQAPRSRALVVHIHGGGFVAQTSKSHEPYLKNWAQELGVPIISIDYSLAPEAPFPRALEECFFAYCWAVKHCELLGSTGERICLAGDSAGGNLCITVSLRAAAYGVRVPDGIMAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTETEDHFDSDQKALGVMGLVQRDTSLFLRDLRLGASSWLNSFLELSGRKPHKTPLPATETLRPTDSGRLTESMRRSVSEAALAQPEGLLGTDSLKKLTIKDLSFKGNSEPSDSPEMSQSMETLGPSTPSDVNFFLRSGNSQEEAETRDDISPMDGIPRVRAAFPDGFHPRRSSQGVLHMPLYSSPIVKNPFMSPLLAPDVMLKTLPPVHLVACALDPMLDDSVMFARRLKDLGQPVTLKVVEDLPHGFLSLAALCRETRQAAELCVQRIRLILTPPAAPLT

Target ORF information:

RefSeq Version	XM_006228393.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228393.2

ORF Insert Sequence:

ATGGTCCTCG CCTCGGCATT CTCACACAGC ATGGATTTAC GCACAATGAC ACAGTCGCTG 
GTGGCGCTCG CAGAAGACAA CATGGCCTTC TTCTCAAGCC AGGGCCCAGG AGAGACAGCA 
CGGCGGCTGT CCAACGTCTT TGCAGGTGTT CGGGAACAGG CACTGGGGCT AGAACCAACT 
CTAGGTCAGC TCTTGGGTGT GGCACACCAT TTCGACCTGG ACACTGAGAC ACCAGCCAAC 
GGATACCGTA GCTTGGTGCA CACAGCTCGT TGCTGCCTGG CACACCTACT ACACAAATCC 
CGCTATGTGG CCTCTAACCG CAGAAGTATC TTCTTCCGTG CCAGCCACAA CCTAGCAGAA 
CTGGAGGCCT ACCTGGCTGC CCTCACCCAG CTCCGTGCTC TAGCCTACTA CGCCCAGCGC 
CTGCTGACCA TCAACCGACC AGGAGTGCTC TTCTTTGAGG GCGATGAGGG ACTCAGCGCT 
GACTTCCTAC AGGACTATGT CACGCTACAT AAAGGCTGCT TTTACGGTCG CTGCCTGGGC 
TTCCAGTTCA CACCTGCCAT CCGGCCGTTC CTGCAGACTC TCTCCATCGG GCTGGTGTCC 
TTCGGGGAAC ACTACAAACG CAACGAGACG GGCCTCAGTG TGACTGCAAG TTCCCTCTTT 
ACGGGTGGCC GATTCGCCAT AGACCCAGAG TTGCGTGGGG CTGAATTTGA GCGCATCATA 
CAGAACCTGG ACGTGCACTT CTGGAAAGCC TTCTGGAATA TCACGGAGAT TGAGGTGCTA 
TCGTCTCTGG CCAACATGGC ATCAACCACC GTGAGGGTAA GCCGCCTGCT TAGCTTGCCG 
CCTGAGGCCT TTGAGATGCC ACTAACCTCT GACCCCAAGC TCACAGTTAC CATCTCACCT 
CCCTTGGCAC ACACGGGACC AGGCCCTGTG CTAGCCAGGC TCATCTCCTA TGACCTGCGG 
GAAGGGCAGG ACAGCAAGAT GCTCAACAGC CTGGCAAAAT CTGAGGGCCC ACGCCTGGAG 
CTGCGCCCAC GGCCTCAACA AGCCCCCCGC TCACGAGCCC TGGTTGTCCA CATTCACGGT 
GGTGGCTTTG TGGCACAGAC CTCCAAATCC CATGAGCCCT ACCTCAAGAA CTGGGCCCAG 
GAGCTAGGCG TCCCCATCAT CTCCATCGAC TACTCCCTGG CCCCTGAGGC CCCCTTCCCC 
CGAGCACTGG AGGAGTGTTT TTTTGCCTAC TGCTGGGCTG TCAAGCACTG TGAACTGCTT 
GGTTCAACCG GAGAGCGGAT ATGCCTTGCG GGGGACAGCG CAGGTGGGAA CCTCTGCATC 
ACCGTGTCCC TTCGGGCAGC AGCCTATGGG GTGAGGGTGC CAGATGGCAT CATGGCAGCC 
TACCCAGTTA CCACCCTGCA GTCCTCTGCT TCTCCCTCTC GTCTGCTGAG CCTCATGGAT 
CCTCTTCTAC CACTGAGCGT ACTCTCCAAG TGTGTGAGCG CCTATTCAGG GACAGAGACG 
GAGGACCATT TTGACTCAGA CCAGAAGGCA TTGGGCGTGA TGGGGCTGGT GCAGAGAGAC 
ACGTCCCTGT TCCTCAGAGA CCTCCGCCTG GGCGCCTCCT CATGGCTCAA CTCCTTCCTG 
GAGTTAAGTG GGCGCAAGCC CCATAAGACC CCATTGCCCG CAACAGAGAC ACTGCGCCCC 
ACGGATTCTG GGCGACTTAC GGAGTCTATG CGCAGGAGTG TGTCTGAGGC AGCCCTGGCC 
CAGCCTGAGG GCTTGCTGGG CACAGATTCC TTGAAGAAGC TAACAATAAA GGACTTGAGC 
TTTAAGGGCA ACTCAGAGCC ATCAGACAGC CCCGAGATGT CACAGTCAAT GGAGACACTT 
GGCCCCTCCA CACCCTCGGA TGTCAACTTT TTTCTGCGAT CCGGGAATTC CCAGGAAGAG 
GCTGAAACCA GAGATGATAT AAGCCCTATG GACGGAATCC CCCGCGTGCG CGCTGCCTTC 
CCTGATGGTT TCCACCCACG GCGCTCAAGC CAAGGTGTCC TCCACATGCC CCTCTACTCG 
TCACCCATAG TCAAGAACCC CTTCATGTCT CCTCTGCTGG CCCCTGACGT CATGCTGAAG 
ACCCTGCCGC CCGTGCACCT TGTGGCGTGC GCTCTGGACC CCATGCTGGA TGACTCGGTC 
ATGTTCGCGC GGCGACTGAA GGACCTGGGC CAGCCCGTGA CGCTGAAAGT GGTAGAGGAC 
CTGCCGCATG GTTTCCTGAG CTTAGCAGCC CTGTGTCGTG AGACCCGGCA GGCCGCGGAG 
TTGTGCGTGC AGCGCATCCG GCTGATCCTC ACCCCCCCTG CTGCACCGCT GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lipe ( XM_006228394.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228456.1 Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X4, mRNA.

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CloneID	ORa22394
Clone ID Related Accession (Same CDS sequence)	XM_006228394.3
Accession Version	XM_006228394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hormone-sensitive lipase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAC GCACAATGAC ACAGTCGCTG GTGGCGCTCG CAGAAGACAA CATGGCCTTC 
TTCTCAAGCC AGGGCCCAGG AGAGACAGCA CGGCGGCTGT CCAACGTCTT TGCAGGTGTT 
CGGGAACAGG CACTGGGGCT AGAACCAACT CTAGGTCAGC TCTTGGGTGT GGCACACCAT 
TTCGACCTGG ACACTGAGAC ACCAGCCAAC GGATACCGTA GCTTGGTGCA CACAGCTCGT 
TGCTGCCTGG CACACCTACT ACACAAATCC CGCTATGTGG CCTCTAACCG CAGAAGTATC 
TTCTTCCGTG CCAGCCACAA CCTAGCAGAA CTGGAGGCCT ACCTGGCTGC CCTCACCCAG 
CTCCGTGCTC TAGCCTACTA CGCCCAGCGC CTGCTGACCA TCAACCGACC AGGAGTGCTC 
TTCTTTGAGG GCGATGAGGG ACTCAGCGCT GACTTCCTAC AGGACTATGT CACGCTACAT 
AAAGGCTGCT TTTACGGTCG CTGCCTGGGC TTCCAGTTCA CACCTGCCAT CCGGCCGTTC 
CTGCAGACTC TCTCCATCGG GCTGGTGTCC TTCGGGGAAC ACTACAAACG CAACGAGACG 
GGCCTCAGTG TGACTGCAAG TTCCCTCTTT ACGGGTGGCC GATTCGCCAT AGACCCAGAG 
TTGCGTGGGG CTGAATTTGA GCGCATCATA CAGAACCTGG ACGTGCACTT CTGGAAAGCC 
TTCTGGAATA TCACGGAGAT TGAGGTGCTA TCGTCTCTGG CCAACATGGC ATCAACCACC 
GTGAGGGTAA GCCGCCTGCT TAGCTTGCCG CCTGAGGCCT TTGAGATGCC ACTAACCTCT 
GACCCCAAGC TCACAGTTAC CATCTCACCT CCCTTGGCAC ACACGGGACC AGGCCCTGTG 
CTAGCCAGGC TCATCTCCTA TGACCTGCGG GAAGGGCAGG ACAGCAAGAT GCTCAACAGC 
CTGGCAAAAT CTGAGGGCCC ACGCCTGGAG CTGCGCCCAC GGCCTCAACA AGCCCCCCGC 
TCACGAGCCC TGGTTGTCCA CATTCACGGT GGTGGCTTTG TGGCACAGAC CTCCAAATCC 
CATGAGCCCT ACCTCAAGAA CTGGGCCCAG GAGCTAGGCG TCCCCATCAT CTCCATCGAC 
TACTCCCTGG CCCCTGAGGC CCCCTTCCCC CGAGCACTGG AGGAGTGTTT TTTTGCCTAC 
TGCTGGGCTG TCAAGCACTG TGAACTGCTT GGTTCAACCG GAGAGCGGAT ATGCCTTGCG 
GGGGACAGCG CAGGTGGGAA CCTCTGCATC ACCGTGTCCC TTCGGGCAGC AGCCTATGGG 
GTGAGGGTGC CAGATGGCAT CATGGCAGCC TACCCAGTTA CCACCCTGCA GTCCTCTGCT 
TCTCCCTCTC GTCTGCTGAG CCTCATGGAT CCTCTTCTAC CACTGAGCGT ACTCTCCAAG 
TGTGTGAGCG CCTATTCAGG GACAGAGACG GAGGACCATT TTGACTCAGA CCAGAAGGCA 
TTGGGCGTGA TGGGGCTGGT GCAGAGAGAC ACGTCCCTGT TCCTCAGAGA CCTCCGCCTG 
GGCGCCTCCT CATGGCTCAA CTCCTTCCTG GAGTTAAGTG GGCGCAAGCC CCATAAGACC 
CCATTGCCCG CAACAGAGAC ACTGCGCCCC ACGGATTCTG GGCGACTTAC GGAGTCTATG 
CGCAGGAGTG TGTCTGAGGC AGCCCTGGCC CAGCCTGAGG GCTTGCTGGG CACAGATTCC 
TTGAAGAAGC TAACAATAAA GGACTTGAGC TTTAAGGGCA ACTCAGAGCC ATCAGACAGC 
CCCGAGATGT CACAGTCAAT GGAGACACTT GGCCCCTCCA CACCCTCGGA TGTCAACTTT 
TTTCTGCGAT CCGGGAATTC CCAGGAAGAG GCTGAAACCA GAGATGATAT AAGCCCTATG 
GACGGAATCC CCCGCGTGCG CGCTGCCTTC CCTGATGGTT TCCACCCACG GCGCTCAAGC 
CAAGGTGTCC TCCACATGCC CCTCTACTCG TCACCCATAG TCAAGAACCC CTTCATGTCT 
CCTCTGCTGG CCCCTGACGT CATGCTGAAG ACCCTGCCGC CCGTGCACCT TGTGGCGTGC 
GCTCTGGACC CCATGCTGGA TGACTCGGTC ATGTTCGCGC GGCGACTGAA GGACCTGGGC 
CAGCCCGTGA CGCTGAAAGT GGTAGAGGAC CTGCCGCATG GTTTCCTGAG CTTAGCAGCC 
CTGTGTCGTG AGACCCGGCA GGCCGCGGAG TTGTGCGTGC AGCGCATCCG GCTGATCCTC 
ACCCCCCCTG CTGCACCGCT GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228456.1
CDS	1804..4110
Translation	MDLRTMTQSLVALAEDNMAFFSSQGPGETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQRLLTINRPGVLFFEGDEGLSADFLQDYVTLHKGCFYGRCLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLFTGGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTSDPKLTVTISPPLAHTGPGPVLARLISYDLREGQDSKMLNSLAKSEGPRLELRPRPQQAPRSRALVVHIHGGGFVAQTSKSHEPYLKNWAQELGVPIISIDYSLAPEAPFPRALEECFFAYCWAVKHCELLGSTGERICLAGDSAGGNLCITVSLRAAAYGVRVPDGIMAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTETEDHFDSDQKALGVMGLVQRDTSLFLRDLRLGASSWLNSFLELSGRKPHKTPLPATETLRPTDSGRLTESMRRSVSEAALAQPEGLLGTDSLKKLTIKDLSFKGNSEPSDSPEMSQSMETLGPSTPSDVNFFLRSGNSQEEAETRDDISPMDGIPRVRAAFPDGFHPRRSSQGVLHMPLYSSPIVKNPFMSPLLAPDVMLKTLPPVHLVACALDPMLDDSVMFARRLKDLGQPVTLKVVEDLPHGFLSLAALCRETRQAAELCVQRIRLILTPPAAPLT

Target ORF information:

RefSeq Version	XM_006228394.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228394.3

ORF Insert Sequence:

ATGGATTTAC GCACAATGAC ACAGTCGCTG GTGGCGCTCG CAGAAGACAA CATGGCCTTC 
TTCTCAAGCC AGGGCCCAGG AGAGACAGCA CGGCGGCTGT CCAACGTCTT TGCAGGTGTT 
CGGGAACAGG CACTGGGGCT AGAACCAACT CTAGGTCAGC TCTTGGGTGT GGCACACCAT 
TTCGACCTGG ACACTGAGAC ACCAGCCAAC GGATACCGTA GCTTGGTGCA CACAGCTCGT 
TGCTGCCTGG CACACCTACT ACACAAATCC CGCTATGTGG CCTCTAACCG CAGAAGTATC 
TTCTTCCGTG CCAGCCACAA CCTAGCAGAA CTGGAGGCCT ACCTGGCTGC CCTCACCCAG 
CTCCGTGCTC TAGCCTACTA CGCCCAGCGC CTGCTGACCA TCAACCGACC AGGAGTGCTC 
TTCTTTGAGG GCGATGAGGG ACTCAGCGCT GACTTCCTAC AGGACTATGT CACGCTACAT 
AAAGGCTGCT TTTACGGTCG CTGCCTGGGC TTCCAGTTCA CACCTGCCAT CCGGCCGTTC 
CTGCAGACTC TCTCCATCGG GCTGGTGTCC TTCGGGGAAC ACTACAAACG CAACGAGACG 
GGCCTCAGTG TGACTGCAAG TTCCCTCTTT ACGGGTGGCC GATTCGCCAT AGACCCAGAG 
TTGCGTGGGG CTGAATTTGA GCGCATCATA CAGAACCTGG ACGTGCACTT CTGGAAAGCC 
TTCTGGAATA TCACGGAGAT TGAGGTGCTA TCGTCTCTGG CCAACATGGC ATCAACCACC 
GTGAGGGTAA GCCGCCTGCT TAGCTTGCCG CCTGAGGCCT TTGAGATGCC ACTAACCTCT 
GACCCCAAGC TCACAGTTAC CATCTCACCT CCCTTGGCAC ACACGGGACC AGGCCCTGTG 
CTAGCCAGGC TCATCTCCTA TGACCTGCGG GAAGGGCAGG ACAGCAAGAT GCTCAACAGC 
CTGGCAAAAT CTGAGGGCCC ACGCCTGGAG CTGCGCCCAC GGCCTCAACA AGCCCCCCGC 
TCACGAGCCC TGGTTGTCCA CATTCACGGT GGTGGCTTTG TGGCACAGAC CTCCAAATCC 
CATGAGCCCT ACCTCAAGAA CTGGGCCCAG GAGCTAGGCG TCCCCATCAT CTCCATCGAC 
TACTCCCTGG CCCCTGAGGC CCCCTTCCCC CGAGCACTGG AGGAGTGTTT TTTTGCCTAC 
TGCTGGGCTG TCAAGCACTG TGAACTGCTT GGTTCAACCG GAGAGCGGAT ATGCCTTGCG 
GGGGACAGCG CAGGTGGGAA CCTCTGCATC ACCGTGTCCC TTCGGGCAGC AGCCTATGGG 
GTGAGGGTGC CAGATGGCAT CATGGCAGCC TACCCAGTTA CCACCCTGCA GTCCTCTGCT 
TCTCCCTCTC GTCTGCTGAG CCTCATGGAT CCTCTTCTAC CACTGAGCGT ACTCTCCAAG 
TGTGTGAGCG CCTATTCAGG GACAGAGACG GAGGACCATT TTGACTCAGA CCAGAAGGCA 
TTGGGCGTGA TGGGGCTGGT GCAGAGAGAC ACGTCCCTGT TCCTCAGAGA CCTCCGCCTG 
GGCGCCTCCT CATGGCTCAA CTCCTTCCTG GAGTTAAGTG GGCGCAAGCC CCATAAGACC 
CCATTGCCCG CAACAGAGAC ACTGCGCCCC ACGGATTCTG GGCGACTTAC GGAGTCTATG 
CGCAGGAGTG TGTCTGAGGC AGCCCTGGCC CAGCCTGAGG GCTTGCTGGG CACAGATTCC 
TTGAAGAAGC TAACAATAAA GGACTTGAGC TTTAAGGGCA ACTCAGAGCC ATCAGACAGC 
CCCGAGATGT CACAGTCAAT GGAGACACTT GGCCCCTCCA CACCCTCGGA TGTCAACTTT 
TTTCTGCGAT CCGGGAATTC CCAGGAAGAG GCTGAAACCA GAGATGATAT AAGCCCTATG 
GACGGAATCC CCCGCGTGCG CGCTGCCTTC CCTGATGGTT TCCACCCACG GCGCTCAAGC 
CAAGGTGTCC TCCACATGCC CCTCTACTCG TCACCCATAG TCAAGAACCC CTTCATGTCT 
CCTCTGCTGG CCCCTGACGT CATGCTGAAG ACCCTGCCGC CCGTGCACCT TGTGGCGTGC 
GCTCTGGACC CCATGCTGGA TGACTCGGTC ATGTTCGCGC GGCGACTGAA GGACCTGGGC 
CAGCCCGTGA CGCTGAAAGT GGTAGAGGAC CTGCCGCATG GTTTCCTGAG CTTAGCAGCC 
CTGTGTCGTG AGACCCGGCA GGCCGCGGAG TTGTGCGTGC AGCGCATCCG GCTGATCCTC 
ACCCCCCCTG CTGCACCGCT GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lipe ( XM_008758895.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757117.1 Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X2, mRNA.

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CloneID	ORa43072
Clone ID Related Accession (Same CDS sequence)	XM_008758895.2
Accession Version	XM_008758895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hormone-sensitive lipase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTG AGTGTGTGGC CTCGGCATTC TCACACAGCA TGGATTTACG CACAATGACA 
CAGTCGCTGG TGGCGCTCGC AGAAGACAAC ATGGCCTTCT TCTCAAGCCA GGGCCCAGGA 
GAGACAGCAC GGCGGCTGTC CAACGTCTTT GCAGGTGTTC GGGAACAGGC ACTGGGGCTA 
GAACCAACTC TAGGTCAGCT CTTGGGTGTG GCACACCATT TCGACCTGGA CACTGAGACA 
CCAGCCAACG GATACCGTAG CTTGGTGCAC ACAGCTCGTT GCTGCCTGGC ACACCTACTA 
CACAAATCCC GCTATGTGGC CTCTAACCGC AGAAGTATCT TCTTCCGTGC CAGCCACAAC 
CTAGCAGAAC TGGAGGCCTA CCTGGCTGCC CTCACCCAGC TCCGTGCTCT AGCCTACTAC 
GCCCAGCGCC TGCTGACCAT CAACCGACCA GGAGTGCTCT TCTTTGAGGG CGATGAGGGA 
CTCAGCGCTG ACTTCCTACA GGACTATGTC ACGCTACATA AAGGCTGCTT TTACGGTCGC 
TGCCTGGGCT TCCAGTTCAC ACCTGCCATC CGGCCGTTCC TGCAGACTCT CTCCATCGGG 
CTGGTGTCCT TCGGGGAACA CTACAAACGC AACGAGACGG GCCTCAGTGT GACTGCAAGT 
TCCCTCTTTA CGGGTGGCCG ATTCGCCATA GACCCAGAGT TGCGTGGGGC TGAATTTGAG 
CGCATCATAC AGAACCTGGA CGTGCACTTC TGGAAAGCCT TCTGGAATAT CACGGAGATT 
GAGGTGCTAT CGTCTCTGGC CAACATGGCA TCAACCACCG TGAGGGTAAG CCGCCTGCTT 
AGCTTGCCGC CTGAGGCCTT TGAGATGCCA CTAACCTCTG ACCCCAAGCT CACAGTTACC 
ATCTCACCTC CCTTGGCACA CACGGGACCA GGCCCTGTGC TAGCCAGGCT CATCTCCTAT 
GACCTGCGGG AAGGGCAGGA CAGCAAGATG CTCAACAGCC TGGCAAAATC TGAGGGCCCA 
CGCCTGGAGC TGCGCCCACG GCCTCAACAA GCCCCCCGCT CACGAGCCCT GGTTGTCCAC 
ATTCACGGTG GTGGCTTTGT GGCACAGACC TCCAAATCCC ATGAGCCCTA CCTCAAGAAC 
TGGGCCCAGG AGCTAGGCGT CCCCATCATC TCCATCGACT ACTCCCTGGC CCCTGAGGCC 
CCCTTCCCCC GAGCACTGGA GGAGTGTTTT TTTGCCTACT GCTGGGCTGT CAAGCACTGT 
GAACTGCTTG GTTCAACCGG AGAGCGGATA TGCCTTGCGG GGGACAGCGC AGGTGGGAAC 
CTCTGCATCA CCGTGTCCCT TCGGGCAGCA GCCTATGGGG TGAGGGTGCC AGATGGCATC 
ATGGCAGCCT ACCCAGTTAC CACCCTGCAG TCCTCTGCTT CTCCCTCTCG TCTGCTGAGC 
CTCATGGATC CTCTTCTACC ACTGAGCGTA CTCTCCAAGT GTGTGAGCGC CTATTCAGGG 
ACAGAGACGG AGGACCATTT TGACTCAGAC CAGAAGGCAT TGGGCGTGAT GGGGCTGGTG 
CAGAGAGACA CGTCCCTGTT CCTCAGAGAC CTCCGCCTGG GCGCCTCCTC ATGGCTCAAC 
TCCTTCCTGG AGTTAAGTGG GCGCAAGCCC CATAAGACCC CATTGCCCGC AACAGAGACA 
CTGCGCCCCA CGGATTCTGG GCGACTTACG GAGTCTATGC GCAGGAGTGT GTCTGAGGCA 
GCCCTGGCCC AGCCTGAGGG CTTGCTGGGC ACAGATTCCT TGAAGAAGCT AACAATAAAG 
GACTTGAGCT TTAAGGGCAA CTCAGAGCCA TCAGACAGCC CCGAGATGTC ACAGTCAATG 
GAGACACTTG GCCCCTCCAC ACCCTCGGAT GTCAACTTTT TTCTGCGATC CGGGAATTCC 
CAGGAAGAGG CTGAAACCAG AGATGATATA AGCCCTATGG ACGGAATCCC CCGCGTGCGC 
GCTGCCTTCC CTGATGGTTT CCACCCACGG CGCTCAAGCC AAGGTGTCCT CCACATGCCC 
CTCTACTCGT CACCCATAGT CAAGAACCCC TTCATGTCTC CTCTGCTGGC CCCTGACGTC 
ATGCTGAAGA CCCTGCCGCC CGTGCACCTT GTGGCGTGCG CTCTGGACCC CATGCTGGAT 
GACTCGGTCA TGTTCGCGCG GCGACTGAAG GACCTGGGCC AGCCCGTGAC GCTGAAAGTG 
GTAGAGGACC TGCCGCATGG TTTCCTGAGC TTAGCAGCCC TGTGTCGTGA GACCCGGCAG 
GCCGCGGAGT TGTGCGTGCA GCGCATCCGG CTGATCCTCA CCCCCCCTGC TGCACCGCTG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757117.1
CDS	169..2514
Translation	MGLECVASAFSHSMDLRTMTQSLVALAEDNMAFFSSQGPGETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQRLLTINRPGVLFFEGDEGLSADFLQDYVTLHKGCFYGRCLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLFTGGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTSDPKLTVTISPPLAHTGPGPVLARLISYDLREGQDSKMLNSLAKSEGPRLELRPRPQQAPRSRALVVHIHGGGFVAQTSKSHEPYLKNWAQELGVPIISIDYSLAPEAPFPRALEECFFAYCWAVKHCELLGSTGERICLAGDSAGGNLCITVSLRAAAYGVRVPDGIMAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTETEDHFDSDQKALGVMGLVQRDTSLFLRDLRLGASSWLNSFLELSGRKPHKTPLPATETLRPTDSGRLTESMRRSVSEAALAQPEGLLGTDSLKKLTIKDLSFKGNSEPSDSPEMSQSMETLGPSTPSDVNFFLRSGNSQEEAETRDDISPMDGIPRVRAAFPDGFHPRRSSQGVLHMPLYSSPIVKNPFMSPLLAPDVMLKTLPPVHLVACALDPMLDDSVMFARRLKDLGQPVTLKVVEDLPHGFLSLAALCRETRQAAELCVQRIRLILTPPAAPLT

Target ORF information:

RefSeq Version	XM_008758895.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipase E, hormone sensitive type (Lipe), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758895.2

ORF Insert Sequence:

ATGGGGCTTG AGTGTGTGGC CTCGGCATTC TCACACAGCA TGGATTTACG CACAATGACA 
CAGTCGCTGG TGGCGCTCGC AGAAGACAAC ATGGCCTTCT TCTCAAGCCA GGGCCCAGGA 
GAGACAGCAC GGCGGCTGTC CAACGTCTTT GCAGGTGTTC GGGAACAGGC ACTGGGGCTA 
GAACCAACTC TAGGTCAGCT CTTGGGTGTG GCACACCATT TCGACCTGGA CACTGAGACA 
CCAGCCAACG GATACCGTAG CTTGGTGCAC ACAGCTCGTT GCTGCCTGGC ACACCTACTA 
CACAAATCCC GCTATGTGGC CTCTAACCGC AGAAGTATCT TCTTCCGTGC CAGCCACAAC 
CTAGCAGAAC TGGAGGCCTA CCTGGCTGCC CTCACCCAGC TCCGTGCTCT AGCCTACTAC 
GCCCAGCGCC TGCTGACCAT CAACCGACCA GGAGTGCTCT TCTTTGAGGG CGATGAGGGA 
CTCAGCGCTG ACTTCCTACA GGACTATGTC ACGCTACATA AAGGCTGCTT TTACGGTCGC 
TGCCTGGGCT TCCAGTTCAC ACCTGCCATC CGGCCGTTCC TGCAGACTCT CTCCATCGGG 
CTGGTGTCCT TCGGGGAACA CTACAAACGC AACGAGACGG GCCTCAGTGT GACTGCAAGT 
TCCCTCTTTA CGGGTGGCCG ATTCGCCATA GACCCAGAGT TGCGTGGGGC TGAATTTGAG 
CGCATCATAC AGAACCTGGA CGTGCACTTC TGGAAAGCCT TCTGGAATAT CACGGAGATT 
GAGGTGCTAT CGTCTCTGGC CAACATGGCA TCAACCACCG TGAGGGTAAG CCGCCTGCTT 
AGCTTGCCGC CTGAGGCCTT TGAGATGCCA CTAACCTCTG ACCCCAAGCT CACAGTTACC 
ATCTCACCTC CCTTGGCACA CACGGGACCA GGCCCTGTGC TAGCCAGGCT CATCTCCTAT 
GACCTGCGGG AAGGGCAGGA CAGCAAGATG CTCAACAGCC TGGCAAAATC TGAGGGCCCA 
CGCCTGGAGC TGCGCCCACG GCCTCAACAA GCCCCCCGCT CACGAGCCCT GGTTGTCCAC 
ATTCACGGTG GTGGCTTTGT GGCACAGACC TCCAAATCCC ATGAGCCCTA CCTCAAGAAC 
TGGGCCCAGG AGCTAGGCGT CCCCATCATC TCCATCGACT ACTCCCTGGC CCCTGAGGCC 
CCCTTCCCCC GAGCACTGGA GGAGTGTTTT TTTGCCTACT GCTGGGCTGT CAAGCACTGT 
GAACTGCTTG GTTCAACCGG AGAGCGGATA TGCCTTGCGG GGGACAGCGC AGGTGGGAAC 
CTCTGCATCA CCGTGTCCCT TCGGGCAGCA GCCTATGGGG TGAGGGTGCC AGATGGCATC 
ATGGCAGCCT ACCCAGTTAC CACCCTGCAG TCCTCTGCTT CTCCCTCTCG TCTGCTGAGC 
CTCATGGATC CTCTTCTACC ACTGAGCGTA CTCTCCAAGT GTGTGAGCGC CTATTCAGGG 
ACAGAGACGG AGGACCATTT TGACTCAGAC CAGAAGGCAT TGGGCGTGAT GGGGCTGGTG 
CAGAGAGACA CGTCCCTGTT CCTCAGAGAC CTCCGCCTGG GCGCCTCCTC ATGGCTCAAC 
TCCTTCCTGG AGTTAAGTGG GCGCAAGCCC CATAAGACCC CATTGCCCGC AACAGAGACA 
CTGCGCCCCA CGGATTCTGG GCGACTTACG GAGTCTATGC GCAGGAGTGT GTCTGAGGCA 
GCCCTGGCCC AGCCTGAGGG CTTGCTGGGC ACAGATTCCT TGAAGAAGCT AACAATAAAG 
GACTTGAGCT TTAAGGGCAA CTCAGAGCCA TCAGACAGCC CCGAGATGTC ACAGTCAATG 
GAGACACTTG GCCCCTCCAC ACCCTCGGAT GTCAACTTTT TTCTGCGATC CGGGAATTCC 
CAGGAAGAGG CTGAAACCAG AGATGATATA AGCCCTATGG ACGGAATCCC CCGCGTGCGC 
GCTGCCTTCC CTGATGGTTT CCACCCACGG CGCTCAAGCC AAGGTGTCCT CCACATGCCC 
CTCTACTCGT CACCCATAGT CAAGAACCCC TTCATGTCTC CTCTGCTGGC CCCTGACGTC 
ATGCTGAAGA CCCTGCCGCC CGTGCACCTT GTGGCGTGCG CTCTGGACCC CATGCTGGAT 
GACTCGGTCA TGTTCGCGCG GCGACTGAAG GACCTGGGCC AGCCCGTGAC GCTGAAAGTG 
GTAGAGGACC TGCCGCATGG TTTCCTGAGC TTAGCAGCCC TGTGTCGTGA GACCCGGCAG 
GCCGCGGAGT TGTGCGTGCA GCGCATCCGG CTGATCCTCA CCCCCCCTGC TGCACCGCTG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lipe ( NM_012859.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036991.1 Rattus norvegicus lipase E, hormone sensitive type (Lipe), mRNA.

Price & Availability↑

CloneID	ORa01140
Clone ID Related Accession (Same CDS sequence)	NM_012859.1
Accession Version	NM_012859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	hormone-sensitive lipase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U40001.1. On Dec 17, 2003 this sequence version replaced XM_346538.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U40001.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAACCTA GGAGACCAAT TTCTTTCACA AGGGAAATAA CAGCTATGGA GCCAAGTTCT 
ACCTCTGTGT CTAGGCCAGA ATGGAGACCT GAGGCACAGC AGACCCTCAC AGACTACCCA 
GGATCAAGGG AACTGCAGGA ATTTGGTATC CCACAGAAAC AAAGTCTCCC AAATGAGGCC 
ACTGCCCAGC AAGGAGCTGA ATTCCAGCAA GAACAGGGAG TTCAACAATC TACTCTGCTC 
CAGAAACTTC TCACCCCATT AGCCTTCCCT GTACCACAGC AATCATTCCC AAGCCACAAA 
GTGCACTCGG ATCAACAGGA AGCCACCTCC CAGAATGGAC CTGGGGCGGG GAAAGTGCAT 
ACAACTCAAA AGGAACTAGA ACACAGAGAT GAACACGTGG GGACAGCAGA ATCTGGACCA 
GCAGAACCAC CTCCAGCTAC AGAAGTAGAG GCTACATCTA TAGCCCAGGC CGTATCTGGA 
CCGGACAAGA AACTCCCTAC CCAAACTGAC TTGGTCTCCC AAGAGAGAGC TGAGCAGTCA 
GACCCTACAG CCCAGCAAAC ACCTCTAGTC CAAGGAGTCA AGTCTGACCA GGGATCTTTG 
ATAGAGTCAG GGATTTTAGC AAGACTGCAG AAACTAGCCA TACAGCAGCC CTCCCAAGAG 
TGGAAGACAT TTCTTGATTG TGTTACAGAG TCTGACATGG AGAAATACCT GAATTCATCC 
TCAAAGTCAA ACCCTCCAGA GCCAAGTGGT GGGACAGTGA TCCCAGGAAC GCTGCCCTCC 
AAACAGAAAC CCGATTGTGG AAAGATGTCA GGATATGGTG GGAAATTACC ACATGGGAAG 
AAAGGCATCC TCCAGAAACA CAAGCACTAC TGGGATACAG CCTCGGCATT CTCACACAGC 
ATGGATTTAC GCACAATGAC ACAGTCGCTG GTGGCGCTCG CAGAAGACAA CATGGCCTTC 
TTCTCAAGCC AGGGCCCAGG AGAGACAGCA CGGCGGCTGT CCAACGTCTT TGCAGGTGTT 
CGGGAACAGG CACTGGGGCT AGAACCAACT CTAGGTCAGC TCTTGGGTGT GGCACACCAT 
TTCGACCTGG ACACTGAGAC ACCAGCCAAC GGATACCGTA GCTTGGTGCA CACAGCTCGT 
TGCTGCCTGG CACACCTACT ACACAAATCC CGCTATGTGG CCTCTAACCG CAGAAGTATC 
TTCTTCCGTG CCAGCCACAA CCTAGCAGAA CTGGAGGCCT ACCTGGCTGC CCTCACCCAG 
CTCCGTGCTC TAGCCTACTA CGCCCAGCGC CTGCTGACCA TCAACCGACC AGGAGTGCTC 
TTCTTTGAGG GCGATGAGGG ACTCAGCGCT GACTTCCTAC AGGACTATGT CACGCTACAT 
AAAGGCTGCT TTTACGGTCG CTGCCTGGGC TTCCAGTTCA CACCTGCCAT CCGGCCGTTC 
CTGCAGACTC TCTCCATCGG GCTGGTGTCC TTCGGGGAAC ACTACAAACG CAACGAGACG 
GGCCTCAGTG TGACTGCAAG TTCCCTCTTT ACGGGTGGCC GATTCGCCAT AGACCCAGAG 
TTGCGTGGGG CTGAATTTGA GCGCATCATA CAGAACCTGG ACGTGCACTT CTGGAAAGCC 
TTCTGGAATA TCACGGAGAT CGAGGTGCTA TCGTCTCTGG CCAACATGGC ATCAACCACC 
GTGAGGGTAA GCCGCCTGCT TAGCTTGCCG CCTGAGGCCT TTGAGATGCC ACTAACCTCT 
GACCCCAAGC TCACAGTTAC CATCTCACCT CCCTTGGCAC ACACGGGACC AGGCCCTGTG 
CTAGCCAGGC TCATCTCCTA TGACCTGCGG GAAGGGCAGG ACAGCAAGAT GCTCAACAGC 
CTGGCAAAAT CTGAGGGCCC ACGCCTGGAG CTGCGCCCAC GGCCTCAACA AGCCCCCCGC 
TCACGAGCCC TGGTTGTCCA CATTCACGGT GGTGGCTTTG TGGCACAGAC CTCCAAATCC 
CATGAGCCCT ACCTCAAGAA CTGGGCCCAG GAGCTAGGCG TCCCCATCAT CTCCATCGAC 
TACTCCCTGG CCCCTGAGGC CCCCTTCCCC CGAGCACTGG AGGAGTGTTT TTTTGCCTAC 
TGCTGGGCTG TCAAGCACTG TGAACTGCTT GGTTCAACCG GAGAGCGGAT ATGCCTTGCG 
GGGGACAGCG CAGGTGGGAA CCTCTGCATC ACCGTGTCCC TTCGGGCAGC AGCCTATGGG 
GTGAGGGTGC CAGATGGCAT CATGGCAGCC TACCCAGTTA CCACCCTGCA GTCCTCTGCT 
TCTCCCTCTC GTCTGCTGAG CCTCATGGAT CCTCTTCTAC CACTGAGCGT ACTCTCCAAG 
TGTGTGAGCG CCTATTCAGG GACAGAGACG GAGGACCATT TTGACTCAGA CCAGAAGGCA 
TTGGGCGTGA TGGGGCTGGT GCAGAGAGAC ACGTCCCTGT TCCTCAGAGA CCTCCGCCTG 
GGCGCCTCCT CATGGCTCAA CTCCTTCCTG GAGTTAAGTG GGCGCAAGCC CCATAAGACC 
CCATTGCCTG CAACAGAGAC ACTGCGCCCC ACGGATTCTG GGCGCCTTAC GGAGTCTATG 
CGCAGGAGTG TGTCTGAGGC AGCCCTGGCC CAGCCTGAGG GCTTGCTGGG CACAGATTCC 
TTGAAGAAGC TAACAATAAA GGACTTGAGC TTTAAGGGCA ACTCAGAGCC ATCAGACAGC 
CCCGAGATGT CACAGTCAAT GGAGACACTT GGCCCCTCCA CACCCTCGGA TGTCAACTTT 
TTTCTGCGAT CCGGGAATTC CCAGGAAGAG GCTGAAACCA GAGATGATAT AAGCCCCATG 
GACGGAATCC CCCGCGTGCG CGCTGCCTTC CCTGATGGTT TCCACCCACG GCGCTCAAGC 
CAAGGTGTCC TCCACATGCC CCTCTACTCG TCACCCATAG TCAAGAACCC CTTCATGTCT 
CCTCTGCTGG CCCCTGACGT CATGCTGAAG ACCCTGCCGC CCGTGCACCT TGTGGCGTGC 
GCTCTGGACC CCATGCTGGA TGACTCGGTC ATGTTCGCGC GGCGACTGAA GGACCTGGGC 
CAGCCCGTGA CGCTGAAAGT GGTAGAGGAC CTGCCGCATG GTTTCCTGAG CTTAGCAGCC 
CTGTGTCGTG AGACCCGGCA GGCCGCGGAG TTGTGCGTGC AGCGCATCCG GCTGATCCTC 
ACCCCCCCTG CTGCACCGCT GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036991.1
CDS	194..3400
Translation	MKPRRPISFTREITAMEPSSTSVSRPEWRPEAQQTLTDYPGSRELQEFGIPQKQSLPNEATAQQGAEFQQEQGVQQSTLLQKLLTPLAFPVPQQSFPSHKVHSDQQEATSQNGPGAGKVHTTQKELEHRDEHVGTAESGPAEPPPATEVEATSIAQAVSGPDKKLPTQTDLVSQERAEQSDPTAQQTPLVQGVKSDQGSLIESGILARLQKLAIQQPSQEWKTFLDCVTESDMEKYLNSSSKSNPPEPSGGTVIPGTLPSKQKPDCGKMSGYGGKLPHGKKGILQKHKHYWDTASAFSHSMDLRTMTQSLVALAEDNMAFFSSQGPGETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQRLLTINRPGVLFFEGDEGLSADFLQDYVTLHKGCFYGRCLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLFTGGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTSDPKLTVTISPPLAHTGPGPVLARLISYDLREGQDSKMLNSLAKSEGPRLELRPRPQQAPRSRALVVHIHGGGFVAQTSKSHEPYLKNWAQELGVPIISIDYSLAPEAPFPRALEECFFAYCWAVKHCELLGSTGERICLAGDSAGGNLCITVSLRAAAYGVRVPDGIMAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTETEDHFDSDQKALGVMGLVQRDTSLFLRDLRLGASSWLNSFLELSGRKPHKTPLPATETLRPTDSGRLTESMRRSVSEAALAQPEGLLGTDSLKKLTIKDLSFKGNSEPSDSPEMSQSMETLGPSTPSDVNFFLRSGNSQEEAETRDDISPMDGIPRVRAAFPDGFHPRRSSQGVLHMPLYSSPIVKNPFMSPLLAPDVMLKTLPPVHLVACALDPMLDDSVMFARRLKDLGQPVTLKVVEDLPHGFLSLAALCRETRQAAELCVQRIRLILTPPAAPLT

Target ORF information:

RefSeq Version	NM_012859.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipase E, hormone sensitive type (Lipe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012859.1

ORF Insert Sequence:

ATGAAACCTA GGAGACCAAT TTCTTTCACA AGGGAAATAA CAGCTATGGA GCCAAGTTCT 
ACCTCTGTGT CTAGGCCAGA ATGGAGACCT GAGGCACAGC AGACCCTCAC AGACTACCCA 
GGATCAAGGG AACTGCAGGA ATTTGGTATC CCACAGAAAC AAAGTCTCCC AAATGAGGCC 
ACTGCCCAGC AAGGAGCTGA ATTCCAGCAA GAACAGGGAG TTCAACAATC TACTCTGCTC 
CAGAAACTTC TCACCCCATT AGCCTTCCCT GTACCACAGC AATCATTCCC AAGCCACAAA 
GTGCACTCGG ATCAACAGGA AGCCACCTCC CAGAATGGAC CTGGGGCGGG GAAAGTGCAT 
ACAACTCAAA AGGAACTAGA ACACAGAGAT GAACACGTGG GGACAGCAGA ATCTGGACCA 
GCAGAACCAC CTCCAGCTAC AGAAGTAGAG GCTACATCTA TAGCCCAGGC CGTATCTGGA 
CCGGACAAGA AACTCCCTAC CCAAACTGAC TTGGTCTCCC AAGAGAGAGC TGAGCAGTCA 
GACCCTACAG CCCAGCAAAC ACCTCTAGTC CAAGGAGTCA AGTCTGACCA GGGATCTTTG 
ATAGAGTCAG GGATTTTAGC AAGACTGCAG AAACTAGCCA TACAGCAGCC CTCCCAAGAG 
TGGAAGACAT TTCTTGATTG TGTTACAGAG TCTGACATGG AGAAATACCT GAATTCATCC 
TCAAAGTCAA ACCCTCCAGA GCCAAGTGGT GGGACAGTGA TCCCAGGAAC GCTGCCCTCC 
AAACAGAAAC CCGATTGTGG AAAGATGTCA GGATATGGTG GGAAATTACC ACATGGGAAG 
AAAGGCATCC TCCAGAAACA CAAGCACTAC TGGGATACAG CCTCGGCATT CTCACACAGC 
ATGGATTTAC GCACAATGAC ACAGTCGCTG GTGGCGCTCG CAGAAGACAA CATGGCCTTC 
TTCTCAAGCC AGGGCCCAGG AGAGACAGCA CGGCGGCTGT CCAACGTCTT TGCAGGTGTT 
CGGGAACAGG CACTGGGGCT AGAACCAACT CTAGGTCAGC TCTTGGGTGT GGCACACCAT 
TTCGACCTGG ACACTGAGAC ACCAGCCAAC GGATACCGTA GCTTGGTGCA CACAGCTCGT 
TGCTGCCTGG CACACCTACT ACACAAATCC CGCTATGTGG CCTCTAACCG CAGAAGTATC 
TTCTTCCGTG CCAGCCACAA CCTAGCAGAA CTGGAGGCCT ACCTGGCTGC CCTCACCCAG 
CTCCGTGCTC TAGCCTACTA CGCCCAGCGC CTGCTGACCA TCAACCGACC AGGAGTGCTC 
TTCTTTGAGG GCGATGAGGG ACTCAGCGCT GACTTCCTAC AGGACTATGT CACGCTACAT 
AAAGGCTGCT TTTACGGTCG CTGCCTGGGC TTCCAGTTCA CACCTGCCAT CCGGCCGTTC 
CTGCAGACTC TCTCCATCGG GCTGGTGTCC TTCGGGGAAC ACTACAAACG CAACGAGACG 
GGCCTCAGTG TGACTGCAAG TTCCCTCTTT ACGGGTGGCC GATTCGCCAT AGACCCAGAG 
TTGCGTGGGG CTGAATTTGA GCGCATCATA CAGAACCTGG ACGTGCACTT CTGGAAAGCC 
TTCTGGAATA TCACGGAGAT CGAGGTGCTA TCGTCTCTGG CCAACATGGC ATCAACCACC 
GTGAGGGTAA GCCGCCTGCT TAGCTTGCCG CCTGAGGCCT TTGAGATGCC ACTAACCTCT 
GACCCCAAGC TCACAGTTAC CATCTCACCT CCCTTGGCAC ACACGGGACC AGGCCCTGTG 
CTAGCCAGGC TCATCTCCTA TGACCTGCGG GAAGGGCAGG ACAGCAAGAT GCTCAACAGC 
CTGGCAAAAT CTGAGGGCCC ACGCCTGGAG CTGCGCCCAC GGCCTCAACA AGCCCCCCGC 
TCACGAGCCC TGGTTGTCCA CATTCACGGT GGTGGCTTTG TGGCACAGAC CTCCAAATCC 
CATGAGCCCT ACCTCAAGAA CTGGGCCCAG GAGCTAGGCG TCCCCATCAT CTCCATCGAC 
TACTCCCTGG CCCCTGAGGC CCCCTTCCCC CGAGCACTGG AGGAGTGTTT TTTTGCCTAC 
TGCTGGGCTG TCAAGCACTG TGAACTGCTT GGTTCAACCG GAGAGCGGAT ATGCCTTGCG 
GGGGACAGCG CAGGTGGGAA CCTCTGCATC ACCGTGTCCC TTCGGGCAGC AGCCTATGGG 
GTGAGGGTGC CAGATGGCAT CATGGCAGCC TACCCAGTTA CCACCCTGCA GTCCTCTGCT 
TCTCCCTCTC GTCTGCTGAG CCTCATGGAT CCTCTTCTAC CACTGAGCGT ACTCTCCAAG 
TGTGTGAGCG CCTATTCAGG GACAGAGACG GAGGACCATT TTGACTCAGA CCAGAAGGCA 
TTGGGCGTGA TGGGGCTGGT GCAGAGAGAC ACGTCCCTGT TCCTCAGAGA CCTCCGCCTG 
GGCGCCTCCT CATGGCTCAA CTCCTTCCTG GAGTTAAGTG GGCGCAAGCC CCATAAGACC 
CCATTGCCTG CAACAGAGAC ACTGCGCCCC ACGGATTCTG GGCGCCTTAC GGAGTCTATG 
CGCAGGAGTG TGTCTGAGGC AGCCCTGGCC CAGCCTGAGG GCTTGCTGGG CACAGATTCC 
TTGAAGAAGC TAACAATAAA GGACTTGAGC TTTAAGGGCA ACTCAGAGCC ATCAGACAGC 
CCCGAGATGT CACAGTCAAT GGAGACACTT GGCCCCTCCA CACCCTCGGA TGTCAACTTT 
TTTCTGCGAT CCGGGAATTC CCAGGAAGAG GCTGAAACCA GAGATGATAT AAGCCCCATG 
GACGGAATCC CCCGCGTGCG CGCTGCCTTC CCTGATGGTT TCCACCCACG GCGCTCAAGC 
CAAGGTGTCC TCCACATGCC CCTCTACTCG TCACCCATAG TCAAGAACCC CTTCATGTCT 
CCTCTGCTGG CCCCTGACGT CATGCTGAAG ACCCTGCCGC CCGTGCACCT TGTGGCGTGC 
GCTCTGGACC CCATGCTGGA TGACTCGGTC ATGTTCGCGC GGCGACTGAA GGACCTGGGC 
CAGCCCGTGA CGCTGAAAGT GGTAGAGGAC CTGCCGCATG GTTTCCTGAG CTTAGCAGCC 
CTGTGTCGTG AGACCCGGCA GGCCGCGGAG TTGTGCGTGC AGCGCATCCG GCTGATCCTC 
ACCCCCCCTG CTGCACCGCT GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes in rat primary white adipocytes.
Biochemical and biophysical research communications464(1)348-53(2015 Aug)
Ogasawara J,Izawa T,Sakurai T,Shirato K,Ishibashi Y,Ohira Y,Ishida H,Ohno H,Kizaki T

Progesterone-induced down-regulation of hormone sensitive lipase (Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis.
The Journal of steroid biochemistry and molecular biology14731-9(2015 Mar)
Stelmanska E,Szrok S,Swierczynski J

Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation.
Physiological reports1(4)e00084(2013 Sep)
Macpherson RE,Vandenboom R,Roy BD,Peters SJ

Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.
Biochemical and biophysical research communications400(1)134-9(2010 Sep)
Ogasawara J,Nomura S,Rahman N,Sakurai T,Kizaki T,Izawa T,Ishida H,Haga S,Ohno H

Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area.
The FEBS journal276(17)4752-62(2009 Sep)
Krintel C,M?rgelin M,Logan DT,Holm C

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Lipe cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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