Prkcb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkcb
Entrez Gene ID	25023
Full Name	protein kinase C, beta
Synonyms	Pkcb,Prkcb1
General protein information	Preferred Names protein kinase C, beta Names protein kinase C beta type PKC-beta protein kinase C beta I protein kinase C beta II protein kinase C, beta 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36
Summary	plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172305.1	NP_001165776.1	protein kinase C beta type isoform 2
NM_012713.4	NP_036845.3	protein kinase C beta type isoform 1
XM_017588799.1	XP_017444288.1	protein kinase C beta type isoform X1

Pathways from BioSystems

Homologies

	PRKCB	XP_001096447.1
Canis lupus familiaris (dog)	PRKCB	XP_005622125.1
Bos taurus (cattle)	PRKCB	NP_777012.1
Rattus norvegicus (Norway rat)	Prkcb	NP_036845.3
Danio rerio (zebrafish)	prkcbb	NP_957272.1
Schizosaccharomyces pombe (fission yeast)	pck1	NP_593737.2
Gallus gallus (chicken)	PRKCB	XP_414868.4
Homo sapiens (human)	PRKCB	NP_002729.2
Mus musculus (house mouse)	Prkcb	NP_032881.1
Xenopus tropicalis (tropical clawed frog)	prkcb	NP_001103513.1
Pan troglodytes (chimpanzee)	PRKCB	XP_003315067.1

Gene Ontology
Bibliography

Related articles in PubMed

TRB3 mediates advanced glycation end product-induced apoptosis of pancreatic ?-cells through the protein kinase C ? pathway.
Wang M, Zhang W, Xu S, Peng L, Wang Z, Liu H, Fang Q, Deng T, Men X, Lou J
International journal of molecular medicine40(1)130-136(2017 Jul)

Diagnostic role of Wnt pathway gene promoter methylation in non small cell lung cancer.
Liu S, Chen X, Chen R, Wang J, Zhu G, Jiang J, Wang H, Duan S, Huang J
Oncotarget8(22)36354-36367(2017 May)

Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.
Malmersj? S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T
The EMBO journal35(16)1810-21(2016 08)

Distinct and complementary roles for ? and ? isoenzymes of PKC in mediating vasoconstrictor responses to acutely elevated glucose.
Jackson R, Brennan S, Fielding P, Sims MW, Challiss RA, Adlam D, Squire IB, Rainbow RD
British journal of pharmacology173(5)870-87(2016 Mar)

A Protein Kinase C Phosphorylation Motif in GLUT1 Affects Glucose Transport and is Mutated in GLUT1 Deficiency Syndrome.
Lee EE, Ma J, Sacharidou A, Mi W, Salato VK, Nguyen N, Jiang Y, Pascual JM, North PE, Shaul PW, Mettlen M, Wang RC
Molecular cell58(5)845-53(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors took a proteomics approach using a PKCB knockout mast cell model and found that the key mast cell secretory protein VAMP8 becomes phosphorylated by PKC at multiple residues in the SNARE domain.
Title: Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.

Our results provide compelling evidence that glucose-induced PKCalpha/PKCbeta-mediated inhibition of Kv current in vascular smooth muscle causes an enhanced constrictor response. Inhibition of Kv current causes a significant depolarization of vascular myocytes leading to marked vasoconstriction
Title: Distinct and complementary roles for ? and ? isoenzymes of PKC in mediating vasoconstrictor responses to acutely elevated glucose.

PKCbeta2 inhibition protects diabetic hearts from myocardial ischemia/reperfusion injury through Cav-3-dependent activation of Akt.
Title: Inhibition of PKC?2 overexpression ameliorates myocardial ischaemia/reperfusion injury in diabetic rats via restoring caveolin-3/Akt signaling.

reduced GLP-1R expression in spontaneously hypertensive rat renal arteries most likely mediated through PKCbeta upregulation
Title: Protein kinase C? mediates downregulated expression of glucagon-like peptide-1 receptor in hypertensive rat renal arteries.

Urotensin-II potentiates ICa,L through the beta-gamma subunits of Gi/o-protein and downstream activation of the class I PI3K-dependent PKCB1 isoform.
Title: Urotensin-II receptor stimulation of cardiac L-type Ca2+ channels requires the ?? subunits of Gi/o-protein and phosphatidylinositol 3-kinase-dependent protein kinase C ?1 isoform.

The following Prkcb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkcb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12200	NM_001172305.1 Latest version!	Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa12212	NM_012713.4 Latest version!	Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$398.30 $569.00
ORa12200	XM_017588799.1 Latest version!	Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkcb ( NM_001172305.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001165776.1 Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12200
Clone ID Related Accession (Same CDS sequence)	NM_001172305.1 , XM_017588799.1
Accession Version	NM_001172305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C beta type isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07005649.1, M19007.1, AABR07005652.1, AABR07005653.1, AABR07005655.1 and CK838368.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC GGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT TTGGGGCTTA 
GGTTTGCAGG GATTCCAGAG TCAGGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAA 
TTCGTCACGT TCTCCTGCCC TGGTGCAGAC AAGGGCCCGG CCTCTGATGA CCCACGGAGC 
AAACACAAGT TTAAGATCCA CACCTACTCC AGCCCTACCT TCTGTGACCA CTGTGGATCA 
CTGCTGTATG GGCTCATCCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAATGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGTGGCACCG ACCACACAGA ACGCCGTGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GAGGTCCTCA TCGTTGTTGT AAGAGATGCT 
AAAAATCTGG TACCTATGGA CCCCAACGGC TTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATCCCTGATC CCAAAAGTGA GAGCAAGCAG AAGACCAAGA CTATCAAATG CTCCCTCAAC 
CCGGAGTGGA ACGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG 
TCCGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCG 
TTTGGGATTT CAGAACTACA GAAAGCCGGA GTGGATGGCT GGTTCAAGTT ACTAAGCCAG 
GAAGAAGGCG AGTACTTTAA TGTGCCGGTG CCGCCGGAAG GAAGCGAGGG CAATGAAGAG 
CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG 
ACAGCGAACA CTATATCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAAGATGT GGTGATCCAA 
GATGACGATG TGGAGTGCAC AATGGTGGAG AAGAGGGTGC TGGCCCTGCC TGGGAAGCCC 
CCATTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTCTA CTTTGTGATG 
GAGTATGTGA ACGGGGGCGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAGATT GCCATCGGTC TTTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AACGTGATGC TGGATTCCGA GGGGCACATC 
AAAATCGCTG ACTTTGGCAT GTGTAAAGAG AATATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATTGCCCCA GAGATCATTG CTTATCAGCC CTACGGGAAG 
TCTGTGGACT GGTGGGCGTT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCT 
TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAGCACAA CGTGGCGTAT 
CCCAAGTCCA TGTCTAAGGA AGCTGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA 
GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCATTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA 
GCTAGAGACA AGCGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGGCA GCCTGTGGAA 
CTGACTCCCA CTGACAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCGTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165776.1
CDS	131..2146
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	NM_001172305.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172305.1

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC GGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT TTGGGGCTTA 
GGTTTGCAGG GATTCCAGAG TCAGGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAA 
TTCGTCACGT TCTCCTGCCC TGGTGCAGAC AAGGGCCCGG CCTCTGATGA CCCACGGAGC 
AAACACAAGT TTAAGATCCA CACCTACTCC AGCCCTACCT TCTGTGACCA CTGTGGATCA 
CTGCTGTATG GGCTCATCCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAATGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGTGGCACCG ACCACACAGA ACGCCGTGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GAGGTCCTCA TCGTTGTTGT AAGAGATGCT 
AAAAATCTGG TACCTATGGA CCCCAACGGC TTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATCCCTGATC CCAAAAGTGA GAGCAAGCAG AAGACCAAGA CTATCAAATG CTCCCTCAAC 
CCGGAGTGGA ACGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG 
TCCGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCG 
TTTGGGATTT CAGAACTACA GAAAGCCGGA GTGGATGGCT GGTTCAAGTT ACTAAGCCAG 
GAAGAAGGCG AGTACTTTAA TGTGCCGGTG CCGCCGGAAG GAAGCGAGGG CAATGAAGAG 
CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG 
ACAGCGAACA CTATATCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAAGATGT GGTGATCCAA 
GATGACGATG TGGAGTGCAC AATGGTGGAG AAGAGGGTGC TGGCCCTGCC TGGGAAGCCC 
CCATTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTCTA CTTTGTGATG 
GAGTATGTGA ACGGGGGCGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAGATT GCCATCGGTC TTTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AACGTGATGC TGGATTCCGA GGGGCACATC 
AAAATCGCTG ACTTTGGCAT GTGTAAAGAG AATATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATTGCCCCA GAGATCATTG CTTATCAGCC CTACGGGAAG 
TCTGTGGACT GGTGGGCGTT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCT 
TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAGCACAA CGTGGCGTAT 
CCCAAGTCCA TGTCTAAGGA AGCTGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA 
GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCATTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA 
GCTAGAGACA AGCGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGGCA GCCTGTGGAA 
CTGACTCCCA CTGACAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCGTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcb ( NM_012713.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036845.3 Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12212
Clone ID Related Accession (Same CDS sequence)	NM_012713.4
Accession Version	NM_012713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C beta type isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07005649.1, AABR07005650.1, AABR07005652.1, AABR07005653.1, AABR07005654.1, AABR07005655.1 and AABR07005656.1. On Jan 6, 2017 this sequence version replaced NM_012713.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC GGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT TTGGGGCTTA 
GGTTTGCAGG GATTCCAGAG TCAGGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAA 
TTCGTCACGT TCTCCTGCCC TGGTGCAGAC AAGGGCCCGG CCTCTGATGA CCCACGGAGC 
AAACACAAGT TTAAGATCCA CACCTACTCC AGCCCTACCT TCTGTGACCA CTGTGGATCA 
CTGCTGTATG GGCTCATCCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAATGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGTGGCACCG ACCACACAGA ACGCCGTGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GAGGTCCTCA TCGTTGTTGT AAGAGATGCT 
AAAAATCTGG TACCTATGGA CCCCAACGGC TTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATCCCTGATC CCAAAAGTGA GAGCAAGCAG AAGACCAAGA CTATCAAATG CTCCCTCAAC 
CCGGAGTGGA ACGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG 
TCCGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCG 
TTTGGGATTT CAGAACTACA GAAAGCCGGA GTGGATGGCT GGTTCAAGTT ACTAAGCCAG 
GAAGAAGGCG AGTACTTTAA TGTGCCGGTG CCGCCGGAAG GAAGCGAGGG CAATGAAGAG 
CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG 
ACAGCGAACA CTATATCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAAGATGT GGTGATCCAA 
GATGACGATG TGGAGTGCAC AATGGTGGAG AAGAGGGTGC TGGCCCTGCC TGGGAAGCCC 
CCATTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTCTA CTTTGTGATG 
GAGTATGTGA ACGGGGGCGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAGATT GCCATCGGTC TTTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AACGTGATGC TGGATTCCGA GGGGCACATC 
AAAATCGCTG ACTTTGGCAT GTGTAAAGAG AATATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATTGCCCCA GAGATCATTG CTTATCAGCC CTACGGGAAG 
TCTGTGGACT GGTGGGCGTT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCT 
TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAGCACAA CGTGGCGTAT 
CCCAAGTCCA TGTCTAAGGA AGCTGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA 
GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCATTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA 
GCTTGTGGGC GAAACGCTGA AAACTTCGAC CGGTTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036845.3
CDS	131..2152
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	NM_012713.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012713.4

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC GGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT TTGGGGCTTA 
GGTTTGCAGG GATTCCAGAG TCAGGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAA 
TTCGTCACGT TCTCCTGCCC TGGTGCAGAC AAGGGCCCGG CCTCTGATGA CCCACGGAGC 
AAACACAAGT TTAAGATCCA CACCTACTCC AGCCCTACCT TCTGTGACCA CTGTGGATCA 
CTGCTGTATG GGCTCATCCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAATGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGTGGCACCG ACCACACAGA ACGCCGTGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GAGGTCCTCA TCGTTGTTGT AAGAGATGCT 
AAAAATCTGG TACCTATGGA CCCCAACGGC TTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATCCCTGATC CCAAAAGTGA GAGCAAGCAG AAGACCAAGA CTATCAAATG CTCCCTCAAC 
CCGGAGTGGA ACGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG 
TCCGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCG 
TTTGGGATTT CAGAACTACA GAAAGCCGGA GTGGATGGCT GGTTCAAGTT ACTAAGCCAG 
GAAGAAGGCG AGTACTTTAA TGTGCCGGTG CCGCCGGAAG GAAGCGAGGG CAATGAAGAG 
CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG 
ACAGCGAACA CTATATCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAAGATGT GGTGATCCAA 
GATGACGATG TGGAGTGCAC AATGGTGGAG AAGAGGGTGC TGGCCCTGCC TGGGAAGCCC 
CCATTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTCTA CTTTGTGATG 
GAGTATGTGA ACGGGGGCGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAGATT GCCATCGGTC TTTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AACGTGATGC TGGATTCCGA GGGGCACATC 
AAAATCGCTG ACTTTGGCAT GTGTAAAGAG AATATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATTGCCCCA GAGATCATTG CTTATCAGCC CTACGGGAAG 
TCTGTGGACT GGTGGGCGTT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCT 
TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAGCACAA CGTGGCGTAT 
CCCAAGTCCA TGTCTAAGGA AGCTGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA 
GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCATTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA 
GCTTGTGGGC GAAACGCTGA AAACTTCGAC CGGTTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcb ( XM_017588799.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444288.1 Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant X1, mRNA.

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CloneID	ORa12200
Clone ID Related Accession (Same CDS sequence)	NM_001172305.1 , XM_017588799.1
Accession Version	XM_017588799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C beta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) and transcript sequence (NM_012713.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 11 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC GGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT TTGGGGCTTA 
GGTTTGCAGG GATTCCAGAG TCAGGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAA 
TTCGTCACGT TCTCCTGCCC TGGTGCAGAC AAGGGCCCGG CCTCTGATGA CCCACGGAGC 
AAACACAAGT TTAAGATCCA CACCTACTCC AGCCCTACCT TCTGTGACCA CTGTGGATCA 
CTGCTGTATG GGCTCATCCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAATGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGTGGCACCG ACCACACAGA ACGCCGTGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GAGGTCCTCA TCGTTGTTGT AAGAGATGCT 
AAAAATCTGG TACCTATGGA CCCCAACGGC TTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATCCCTGATC CCAAAAGTGA GAGCAAGCAG AAGACCAAGA CTATCAAATG CTCCCTCAAC 
CCGGAGTGGA ACGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG 
TCCGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCG 
TTTGGGATTT CAGAACTACA GAAAGCCGGA GTGGATGGCT GGTTCAAGTT ACTAAGCCAG 
GAAGAAGGCG AGTACTTTAA TGTGCCGGTG CCGCCGGAAG GAAGCGAGGG CAATGAAGAG 
CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG 
ACAGCGAACA CTATATCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAAGATGT GGTGATCCAA 
GATGACGATG TGGAGTGCAC AATGGTGGAG AAGAGGGTGC TGGCCCTGCC TGGGAAGCCC 
CCATTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTCTA CTTTGTGATG 
GAGTATGTGA ACGGGGGCGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAGATT GCCATCGGTC TTTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AACGTGATGC TGGATTCCGA GGGGCACATC 
AAAATCGCTG ACTTTGGCAT GTGTAAAGAG AATATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATTGCCCCA GAGATCATTG CTTATCAGCC CTACGGGAAG 
TCTGTGGACT GGTGGGCGTT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCT 
TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAGCACAA CGTGGCGTAT 
CCCAAGTCCA TGTCTAAGGA AGCTGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA 
GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCATTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA 
GCTAGAGACA AGCGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGGCA GCCTGTGGAA 
CTGACTCCCA CTGACAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCGTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444288.1
CDS	342..2357
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	XM_017588799.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, beta (Prkcb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588799.1

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC GGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT TTGGGGCTTA 
GGTTTGCAGG GATTCCAGAG TCAGGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAA 
TTCGTCACGT TCTCCTGCCC TGGTGCAGAC AAGGGCCCGG CCTCTGATGA CCCACGGAGC 
AAACACAAGT TTAAGATCCA CACCTACTCC AGCCCTACCT TCTGTGACCA CTGTGGATCA 
CTGCTGTATG GGCTCATCCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAATGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGTGGCACCG ACCACACAGA ACGCCGTGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GAGGTCCTCA TCGTTGTTGT AAGAGATGCT 
AAAAATCTGG TACCTATGGA CCCCAACGGC TTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATCCCTGATC CCAAAAGTGA GAGCAAGCAG AAGACCAAGA CTATCAAATG CTCCCTCAAC 
CCGGAGTGGA ACGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG 
TCCGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCG 
TTTGGGATTT CAGAACTACA GAAAGCCGGA GTGGATGGCT GGTTCAAGTT ACTAAGCCAG 
GAAGAAGGCG AGTACTTTAA TGTGCCGGTG CCGCCGGAAG GAAGCGAGGG CAATGAAGAG 
CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG 
ACAGCGAACA CTATATCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAAGATGT GGTGATCCAA 
GATGACGATG TGGAGTGCAC AATGGTGGAG AAGAGGGTGC TGGCCCTGCC TGGGAAGCCC 
CCATTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTCTA CTTTGTGATG 
GAGTATGTGA ACGGGGGCGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAGATT GCCATCGGTC TTTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AACGTGATGC TGGATTCCGA GGGGCACATC 
AAAATCGCTG ACTTTGGCAT GTGTAAAGAG AATATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATTGCCCCA GAGATCATTG CTTATCAGCC CTACGGGAAG 
TCTGTGGACT GGTGGGCGTT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCT 
TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAGCACAA CGTGGCGTAT 
CCCAAGTCCA TGTCTAAGGA AGCTGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA 
GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCATTTTTC 
CGGTATATCG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA 
GCTAGAGACA AGCGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGGCA GCCTGTGGAA 
CTGACTCCCA CTGACAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCGTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRB3 mediates advanced glycation end product-induced apoptosis of pancreatic ?-cells through the protein kinase C ? pathway.
International journal of molecular medicine40(1)130-136(2017 Jul)
Wang M,Zhang W,Xu S,Peng L,Wang Z,Liu H,Fang Q,Deng T,Men X,Lou J

Diagnostic role of Wnt pathway gene promoter methylation in non small cell lung cancer.
Oncotarget8(22)36354-36367(2017 May)
Liu S,Chen X,Chen R,Wang J,Zhu G,Jiang J,Wang H,Duan S,Huang J

Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.
The EMBO journal35(16)1810-21(2016 08)
Malmersj? S,Di Palma S,Diao J,Lai Y,Pfuetzner RA,Wang AL,McMahon MA,Hayer A,Porteus M,Bodenmiller B,Brunger AT,Meyer T

Distinct and complementary roles for ? and ? isoenzymes of PKC in mediating vasoconstrictor responses to acutely elevated glucose.
British journal of pharmacology173(5)870-87(2016 Mar)
Jackson R,Brennan S,Fielding P,Sims MW,Challiss RA,Adlam D,Squire IB,Rainbow RD

A Protein Kinase C Phosphorylation Motif in GLUT1 Affects Glucose Transport and is Mutated in GLUT1 Deficiency Syndrome.
Molecular cell58(5)845-53(2015 Jun)
Lee EE,Ma J,Sacharidou A,Mi W,Salato VK,Nguyen N,Jiang Y,Pascual JM,North PE,Shaul PW,Mettlen M,Wang RC

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Prkcb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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