PRKCB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCB
Entrez Gene ID	5579
Full Name	protein kinase C beta
Synonyms	PKC-beta,PKCB,PRKCB1,PRKCB2
General protein information	Preferred Names protein kinase C beta Names protein kinase C beta type PKC-B protein kinase C, beta 1 polypeptide
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.2-p12.1
Summary	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	176970

mRNA and Protein(s)

mRNA	Protein	Name
NM_212535.3	NP_997700.1	protein kinase C beta type isoform 1
NM_002738.7	NP_002729.2	protein kinase C beta type isoform 2
NM_212535.2	NP_997700.1	protein kinase C beta type isoform 1
NM_002738.6	NP_002729.2	protein kinase C beta type isoform 2

Pathways from BioSystems

Homologies

	PRKCB	XP_001096447.1
Canis lupus familiaris (dog)	PRKCB	XP_005622125.1
Bos taurus (cattle)	PRKCB	NP_777012.1
Rattus norvegicus (Norway rat)	Prkcb	NP_036845.3
Danio rerio (zebrafish)	prkcbb	NP_957272.1
Schizosaccharomyces pombe (fission yeast)	pck1	NP_593737.2
Gallus gallus (chicken)	PRKCB	XP_414868.4
Homo sapiens (human)	PRKCB	NP_002729.2
Mus musculus (house mouse)	Prkcb	NP_032881.1
Xenopus tropicalis (tropical clawed frog)	prkcb	NP_001103513.1
Pan troglodytes (chimpanzee)	PRKCB	XP_003315067.1

Gene Ontology

The following PRKCB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17455	NM_212535.3 Latest version!	Homo sapiens protein kinase C beta (PRKCB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu18081	NM_002738.7 Latest version!	Homo sapiens protein kinase C beta (PRKCB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu17455	NM_212535.2	Homo sapiens protein kinase C beta (PRKCB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu18081	NM_002738.6	Homo sapiens protein kinase C beta (PRKCB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCB ( NM_212535.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_997700.1 Homo sapiens protein kinase C beta (PRKCB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17455
Clone ID Related Accession (Same CDS sequence)	NM_212535.3 , NM_212535.2
Accession Version	NM_212535.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	protein kinase C beta type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456157.1, X06318.1, BC036472.1 and AC130448.2. On Jun 2, 2019 this sequence version replaced NM_212535.2. Transcript Variant: This variant (1) uses an alternate splice junction at the 5' end of the last exon compared to variant 2. The resulting isoform (1) has a distinct and shorter C-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.196009.1, SRR1803612.49090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997700.1
CDS	194..2209
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	NM_212535.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C beta (PRKCB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212535.3

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( NM_212535.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997700.1 Homo sapiens protein kinase C beta (PRKCB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17455
Clone ID Related Accession (Same CDS sequence)	NM_212535.3 , NM_212535.2
Accession Version	NM_212535.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	protein kinase C beta type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456157.1, X06318.1, BC036472.1 and AC130448.2. On Aug 20, 2008 this sequence version replaced NM_212535.1. Transcript Variant: This variant (1) uses an alternate splice junction at the 5' end of the last exon compared to variant 2. The resulting isoform (1) has a distinct and shorter C-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X06318.1, SRR1660809.28532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997700.1
CDS	198..2213
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	NM_212535.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C beta (PRKCB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212535.2

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( NM_002738.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_002729.2 Homo sapiens protein kinase C beta (PRKCB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18081
Clone ID Related Accession (Same CDS sequence)	NM_002738.7 , NM_002738.6
Accession Version	NM_002738.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	protein kinase C beta type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456157.1, BC036472.1 and AC130448.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_002738.6. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.243143.1, SRR1803617.171461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCCG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002729.2
CDS	194..2215
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	NM_002738.7
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C beta (PRKCB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002738.7

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCCG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( NM_002738.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002729.2 Homo sapiens protein kinase C beta (PRKCB), transcript variant 2, mRNA.

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CloneID	OHu18081
Clone ID Related Accession (Same CDS sequence)	NM_002738.7 , NM_002738.6
Accession Version	NM_002738.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	protein kinase C beta type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456157.1, BC036472.1 and AC130448.2. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2008 this sequence version replaced NM_002738.5. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X07109.1, SRR1803617.171461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCCG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002729.2
CDS	198..2219
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	NM_002738.6
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C beta (PRKCB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002738.6

ORF Insert Sequence:

ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA 
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC 
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC 
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG 
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC 
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG 
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC 
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA 
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG 
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC 
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA 
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA 
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG 
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG 
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG 
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG 
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC 
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA 
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC 
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT 
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA 
GCTTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA 
ACACCTCCCG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PRKCB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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