prkcb cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkcb
Entrez Gene ID	100038043
Full Name	protein kinase C beta
Synonyms	PKC-B,PKCbeta,XPKCII,pkc-beta,pkcb,prkcb1,prkcb2,prkcbb,xPKCb
General protein information	Preferred Names protein kinase C beta Names protein kinase C beta type pkc betaII protein kinase C, beta 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012969929.2	XP_012825383.1	protein kinase C beta type isoform X1
XM_012969929.3	XP_012825383.1	protein kinase C beta type isoform X1
NM_001110043.1	NP_001103513.1	protein kinase C beta type

Pathways from BioSystems

Homologies

	PRKCB	XP_001096447.1
Canis lupus familiaris (dog)	PRKCB	XP_005622125.1
Bos taurus (cattle)	PRKCB	NP_777012.1
Rattus norvegicus (Norway rat)	Prkcb	NP_036845.3
Danio rerio (zebrafish)	prkcbb	NP_957272.1
Schizosaccharomyces pombe (fission yeast)	pck1	NP_593737.2
Gallus gallus (chicken)	PRKCB	XP_414868.4
Homo sapiens (human)	PRKCB	NP_002729.2
Mus musculus (house mouse)	Prkcb	NP_032881.1
Xenopus tropicalis (tropical clawed frog)	prkcb	NP_001103513.1
Pan troglodytes (chimpanzee)	PRKCB	XP_003315067.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prkcb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkcb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa00836	NM_001110043.1 Latest version!	Xenopus tropicalis protein kinase C beta (prkcb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa30019	XM_012969929.2 Latest version!	Xenopus tropicalis protein kinase C, beta (prkcb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa65788	XM_012969929.3 Latest version!	Xenopus tropicalis protein kinase C beta (prkcb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkcb ( NM_001110043.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001103513.1 Xenopus tropicalis protein kinase C beta (prkcb), mRNA.

Price & Availability↑

CloneID	OXa00836
Clone ID Related Accession (Same CDS sequence)	NM_001110043.1
Accession Version	NM_001110043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein kinase C beta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154114.1. ##Evidence-Data-START## Transcript exon combination :: BC154114.1, BC121499.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGACC CCGCAGCCTG CGAGCCTGGG GAGGACACCA CCACTCGCTT TGCCAGGAAG 
GGGGCTCTGC GGCAGAAGAA TGTGCATGAG GTGAAGGAGC ACAAGTTCAC AGCCCGCTTC 
TTCAAGCAGC CCACCTTCTG CAGCCACTGC ACCGACTTCA TCTGGGGTTT TGGCAAACAG 
GGATTCCAGT GTCAAGTTTG CTGTTTCGTG GTGCACAAGC GGTGTCATGA GTTTGTCACG 
TTTTCCTGCC CGGGCGCAGA TAAAGGGCCA GCATCTGATG ACCCCCGCAG CAAACACAAG 
TTCAAAATTC ACACGTACTC CAGCCCGACG TTCTGTGACC ACTGCGGCTC ATTGCTGTAC 
GGCCTCATTC ACCAGGGGAT GAAATGTGAA ACCTGCATGA TGAATGTCCA CAAGCGCTGC 
GTAATGAACG TGCCAAGTCT GTGTGGCACC GACCACACCG AGCGAAGAGG AAGGATACAC 
ATCAAGGCGG AGATCCGCGA GGAAGTACTG ACAGTCACTG TCGGGGATGC GAGAAACCTA 
GTTCCAATGG ACCCAAATGG ACTGTCTGAC CCGTACGTAA AGCTAAAGCT CATCCCAGAC 
CCAAAGAGCG AGAGCAAACA AAAGACCAAG ACTATCAAGT GTTCCCTGAA CCCAACCTGG 
AATGAGAGCT TTAAATTCCA GCTGAAGGAG TCCGATAAAG ATCGCAGGCT GTCCGTGGAA 
ATCTGGGACT GGGACTTAAC CAGTAGGAAT GATTTCATGG GGTCCCTGTC GTTTGGTATC 
TCGGAGCTAC TGAAGGCCGG CGTAGACGGT TGGTTCAAGT TGCTCAGCCA AGAGGAGGGA 
GAATACTTTA ACGTACCTGT GCCACCTGAA GGAGAAGAAG GCAACGAAGA GCTACGCCAG 
AAGTTTGAGA GAGCTAAAAT TGGCCCCGGG ACCAAAGCCG TTGAAGAGAA AGTGGTGAAC 
CCAATGCCCA AGGTGGACAA CAATGAAACC AGGGACCGTA TGAAAGTGTC AGACTTTAAC 
TTCCTAAAGG TTCTGGGGAA AGGCAGCTTT GGGAAGGTGA TCCTGGCAGA GAGGAAAGGA 
ACAGATGAGC TCTATGCAAT AAAGATCCTG AAGAAGGACG TGGTGATTCA GGATGATGAT 
GTTGAGTGCA CAATGGTAGA GAAGAGGGTC TTGGCTCTGT CTGGGAAGCC CCCATTCCTC 
ACTCAGCTGC ACTCCTGTTT CCAGACAATG GATCGCCTTT ACTTTGTCAT GGAGTACGTC 
AATGGTGGAG ACCTCATGTA CCAGATCCAG CAGGTCGGCC GATTCAAGGA ACCACATGCA 
GTATTCTACG CAGCAGAGAT AGCCATCGGC CTGCTATTCC TGCACAGCAA AGGCATCGTG 
TATAGGGACC TCAAGCTGGA TAACGTCATG CTGGATTCAG AGGGACACAT AAAGATCGCA 
GATTTCGGCA TGTGTAAGGA GAACATGTGG GACGGGGTAA CGACAAAGAC CTTCTGCGGC 
ACGCCAGACT ACATAGCGCC CGAGATTATA GCTTACCAGC CTTACGCCAA GTCAGTGGAT 
TGGTGGGCAT TCGGGATCCT TCTTTACGAG ATGTTAGCCG GGCAGGCTCC GTTCGAAGGG 
GAAGATGAGG ATGAGTTATT CCAGTCCATC ATGGAGCATA ATGTAGCATA TCCCAAATCA 
ATGTCCAAGG AAGCTGTGGC CATCTGTAAA GGGCTGATGA CAAAGCATCC TGGGAAGCGG 
CTGGGCTGTG GCCCAGAGGG GGAGCGAGAC ATTAAGGACC ATGCCTTTTT CCGATACATA 
GACTGGGAGA AGCTGGAAAG GAACGAGATC CAACCCCCCT ACAAGCCAAA GGCGTGCGGC 
CGAAATGCTG AGAACTTCGA CAAGTTTTTT ACCCGCCACC CACCAGTGCT CACGCCACCC 
GACCATGAGG TCATCCGGAA CATTGACCAA TCAGAATTTG AAGGCTTTTC CTATGTTAAC 
TCTGATTTTG CAAAAGAGGA GGAGAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103513.1
CDS	84..2096
Translation	MADPAACEPGEDTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLCGTDHTERRGRIHIKAEIREEVLTVTVGDARNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPTWNESFKFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELLKAGVDGWFKLLSQEEGEYFNVPVPPEGEEGNEELRQKFERAKIGPGTKAVEEKVVNPMPKVDNNETRDRMKVSDFNFLKVLGKGSFGKVILAERKGTDELYAIKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYQIQQVGRFKEPHAVFYAAEIAIGLLFLHSKGIVYRDLKLDNVMLDSEGHIKIADFGMCKENMWDGVTTKTFCGTPDYIAPEIIAYQPYAKSVDWWAFGILLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKDHAFFRYIDWEKLERNEIQPPYKPKACGRNAENFDKFFTRHPPVLTPPDHEVIRNIDQSEFEGFSYVNSDFAKEEEKD

Target ORF information:

RefSeq Version	NM_001110043.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase C beta (prkcb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110043.1

ORF Insert Sequence:

ATGGCTGACC CCGCAGCCTG CGAGCCTGGG GAGGACACCA CCACTCGCTT TGCCAGGAAG 
GGGGCTCTGC GGCAGAAGAA TGTGCATGAG GTGAAGGAGC ACAAGTTCAC AGCCCGCTTC 
TTCAAGCAGC CCACCTTCTG CAGCCACTGC ACCGACTTCA TCTGGGGTTT TGGCAAACAG 
GGATTCCAGT GTCAAGTTTG CTGTTTCGTG GTGCACAAGC GGTGTCATGA GTTTGTCACG 
TTTTCCTGCC CGGGCGCAGA TAAAGGGCCA GCATCTGATG ACCCCCGCAG CAAACACAAG 
TTCAAAATTC ACACGTACTC CAGCCCGACG TTCTGTGACC ACTGCGGCTC ATTGCTGTAC 
GGCCTCATTC ACCAGGGGAT GAAATGTGAA ACCTGCATGA TGAATGTCCA CAAGCGCTGC 
GTAATGAACG TGCCAAGTCT GTGTGGCACC GACCACACCG AGCGAAGAGG AAGGATACAC 
ATCAAGGCGG AGATCCGCGA GGAAGTACTG ACAGTCACTG TCGGGGATGC GAGAAACCTA 
GTTCCAATGG ACCCAAATGG ACTGTCTGAC CCGTACGTAA AGCTAAAGCT CATCCCAGAC 
CCAAAGAGCG AGAGCAAACA AAAGACCAAG ACTATCAAGT GTTCCCTGAA CCCAACCTGG 
AATGAGAGCT TTAAATTCCA GCTGAAGGAG TCCGATAAAG ATCGCAGGCT GTCCGTGGAA 
ATCTGGGACT GGGACTTAAC CAGTAGGAAT GATTTCATGG GGTCCCTGTC GTTTGGTATC 
TCGGAGCTAC TGAAGGCCGG CGTAGACGGT TGGTTCAAGT TGCTCAGCCA AGAGGAGGGA 
GAATACTTTA ACGTACCTGT GCCACCTGAA GGAGAAGAAG GCAACGAAGA GCTACGCCAG 
AAGTTTGAGA GAGCTAAAAT TGGCCCCGGG ACCAAAGCCG TTGAAGAGAA AGTGGTGAAC 
CCAATGCCCA AGGTGGACAA CAATGAAACC AGGGACCGTA TGAAAGTGTC AGACTTTAAC 
TTCCTAAAGG TTCTGGGGAA AGGCAGCTTT GGGAAGGTGA TCCTGGCAGA GAGGAAAGGA 
ACAGATGAGC TCTATGCAAT AAAGATCCTG AAGAAGGACG TGGTGATTCA GGATGATGAT 
GTTGAGTGCA CAATGGTAGA GAAGAGGGTC TTGGCTCTGT CTGGGAAGCC CCCATTCCTC 
ACTCAGCTGC ACTCCTGTTT CCAGACAATG GATCGCCTTT ACTTTGTCAT GGAGTACGTC 
AATGGTGGAG ACCTCATGTA CCAGATCCAG CAGGTCGGCC GATTCAAGGA ACCACATGCA 
GTATTCTACG CAGCAGAGAT AGCCATCGGC CTGCTATTCC TGCACAGCAA AGGCATCGTG 
TATAGGGACC TCAAGCTGGA TAACGTCATG CTGGATTCAG AGGGACACAT AAAGATCGCA 
GATTTCGGCA TGTGTAAGGA GAACATGTGG GACGGGGTAA CGACAAAGAC CTTCTGCGGC 
ACGCCAGACT ACATAGCGCC CGAGATTATA GCTTACCAGC CTTACGCCAA GTCAGTGGAT 
TGGTGGGCAT TCGGGATCCT TCTTTACGAG ATGTTAGCCG GGCAGGCTCC GTTCGAAGGG 
GAAGATGAGG ATGAGTTATT CCAGTCCATC ATGGAGCATA ATGTAGCATA TCCCAAATCA 
ATGTCCAAGG AAGCTGTGGC CATCTGTAAA GGGCTGATGA CAAAGCATCC TGGGAAGCGG 
CTGGGCTGTG GCCCAGAGGG GGAGCGAGAC ATTAAGGACC ATGCCTTTTT CCGATACATA 
GACTGGGAGA AGCTGGAAAG GAACGAGATC CAACCCCCCT ACAAGCCAAA GGCGTGCGGC 
CGAAATGCTG AGAACTTCGA CAAGTTTTTT ACCCGCCACC CACCAGTGCT CACGCCACCC 
GACCATGAGG TCATCCGGAA CATTGACCAA TCAGAATTTG AAGGCTTTTC CTATGTTAAC 
TCTGATTTTG CAAAAGAGGA GGAGAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcb ( XM_012969929.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825383.1 Xenopus tropicalis protein kinase C, beta (prkcb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30019
Clone ID Related Accession (Same CDS sequence)	XM_012969929.2
Accession Version	XM_012969929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein kinase C beta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CCGCAGCCTG CGAGCCTGGG GAGGACACCA CCACTCGCTT TGCCAGGAAG 
GGGGCTCTGC GGCAGAAGAA TGTGCATGAG GTGAAGGAGC ACAAGTTCAC AGCCCGCTTC 
TTCAAGCAGC CCACCTTCTG CAGCCACTGC ACCGACTTCA TCTGGGGTTT TGGCAAACAG 
GGATTCCAGT GTCAAGTTTG CTGTTTCGTG GTGCACAAGC GGTGTCATGA GTTTGTCACG 
TTTTCCTGCC CGGGCGCAGA TAAAGGGCCA GCATCTGATG ACCCCCGCAG CAAACACAAG 
TTCAAAATTC ACACGTACTC CAGCCCGACG TTCTGTGACC ACTGCGGCTC ATTGCTGTAC 
GGCCTCATTC ACCAGGGGAT GAAATGTGAA ACCTGCATGA TGAATGTCCA CAAGCGCTGC 
GTAATGAACG TGCCAAGTCT GTGTGGCACC GACCACACCG AGCGAAGAGG AAGGATACAC 
ATCAAGGCGG AGATCCGCGA GGAAGTACTG ACAGTCACTG TCGGGGATGC GAGAAACCTA 
GTTCCAATGG ACCCAAATGG ACTGTCTGAC CCGTACGTAA AGCTAAAGCT CATCCCAGAC 
CCAAAGAGCG AGAGCAAACA AAAGACCAAG ACTATCAAGT GTTCCCTGAA CCCAACCTGG 
AATGAGAGCT TTAAATTCCA GCTGAAGGAG TCCGATAAAG ATCGCAGGCT GTCCGTGGAA 
ATCTGGGACT GGGACTTAAC CAGTAGGAAT GATTTCATGG GGTCCCTGTC GTTTGGTATC 
TCGGAGCTAC TGAAGGCCGG CGTAGACGGT TGGTTCAAGT TGCTCAGCCA AGAGGAGGGA 
GAATACTTTA ACGTACCTGT GCCACCTGAA GGAGAAGAAG GCAACGAAGA GCTACGCCAG 
AAGTTTGAGA GAGCTAAAAT TGGCCCCGGG ACCAAAGCCG TTGAAGAGAA AGTGGTGAAC 
CCAATGCCCA AGGTGGACAA CAATGGAACC AGGGACCGTA TGAAAGTGTC AGACTTTAAC 
TTCCTAAAGG TTCTGGGGAA AGGCAGCTTT GGGAAGGTGA TCCTGGCAGA GAGGAAAGGA 
ACAGATGAGC TCTATGCAAT AAAGATCCTG AAGAAGGACG TGGTGATTCA GGATGATGAT 
GTTGAGTGCA CAATGGTAGA GAAGAGGGTC TTGGCTCTGT CTGGGAAGCC CCCATTCCTC 
ACTCAGCTGC ACTCCTGTTT CCAGACAATG GATCGCCTTT ACTTTGTCAT GGAGTACGTC 
AATGGTGGAG ACCTCATGTA CCAGATCCAG CAGGTCGGCC GATTCAAGGA ACCACATGCA 
GTATTCTACG CAGCAGAGAT AGCCATCGGC CTGCTATTCC TGCACAGCAA AGGCATCGTG 
TATAGGGACC TCAAGCTGGA TAACGTCATG CTGGATTCAG AGGGACACAT AAAGATCGCA 
GATTTCGGCA TGTGTAAGGA GAACATGTGG GACGGGGTAA CGACAAAGAC CTTCTGCGGC 
ACGCCAGACT ACATAGCGCC CGAGATTATA GCTTACCAGC CTTACGCCAA GTCAGTGGAT 
TGGTGGGCAT TCGGGATCCT TCTTTACGAG ATGTTAGCCG GGCAGGCTCC GTTCGAAGGG 
GAAGATGAGG ATGAGTTATT CCAGTCCATC ATGGAGCATA ATGTAGCATA TCCCAAATCA 
ATGTCCAAGG AAGCTGTGGC CATCTGTAAA GGGCTGATGA CAAAGCATCC TGGGAAGCGG 
CTGGGCTGTG GCCCAGAGGG GGAGCGAGAC ATTAAGGACC ATGCCTTTTT CCGATACATA 
GACTGGGAGA AGCTGGAAAG GAACGAGATC CAACCCCCCT ACAAGCCAAA GGCGAAGGAC 
AGGAAGGACA CCTCTAATTT TGACAAGGAG TTCACCCGCC AGCCCGTCGA GCTCACCCCC 
ACCGACAAAC TCTTTATCAT GAACCTGGAT CAAAATGAGT TTGCCGGATT CTCCTACGTC 
AACCCAGAGT TTGTGATCGT CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825383.1
CDS	123..2129
Translation	MADPAACEPGEDTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLCGTDHTERRGRIHIKAEIREEVLTVTVGDARNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPTWNESFKFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELLKAGVDGWFKLLSQEEGEYFNVPVPPEGEEGNEELRQKFERAKIGPGTKAVEEKVVNPMPKVDNNGTRDRMKVSDFNFLKVLGKGSFGKVILAERKGTDELYAIKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYQIQQVGRFKEPHAVFYAAEIAIGLLFLHSKGIVYRDLKLDNVMLDSEGHIKIADFGMCKENMWDGVTTKTFCGTPDYIAPEIIAYQPYAKSVDWWAFGILLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKDHAFFRYIDWEKLERNEIQPPYKPKAKDRKDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYVNPEFVIVV

Target ORF information:

RefSeq Version	XM_012969929.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase C, beta (prkcb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969929.2

ORF Insert Sequence:

ATGGCTGACC CCGCAGCCTG CGAGCCTGGG GAGGACACCA CCACTCGCTT TGCCAGGAAG 
GGGGCTCTGC GGCAGAAGAA TGTGCATGAG GTGAAGGAGC ACAAGTTCAC AGCCCGCTTC 
TTCAAGCAGC CCACCTTCTG CAGCCACTGC ACCGACTTCA TCTGGGGTTT TGGCAAACAG 
GGATTCCAGT GTCAAGTTTG CTGTTTCGTG GTGCACAAGC GGTGTCATGA GTTTGTCACG 
TTTTCCTGCC CGGGCGCAGA TAAAGGGCCA GCATCTGATG ACCCCCGCAG CAAACACAAG 
TTCAAAATTC ACACGTACTC CAGCCCGACG TTCTGTGACC ACTGCGGCTC ATTGCTGTAC 
GGCCTCATTC ACCAGGGGAT GAAATGTGAA ACCTGCATGA TGAATGTCCA CAAGCGCTGC 
GTAATGAACG TGCCAAGTCT GTGTGGCACC GACCACACCG AGCGAAGAGG AAGGATACAC 
ATCAAGGCGG AGATCCGCGA GGAAGTACTG ACAGTCACTG TCGGGGATGC GAGAAACCTA 
GTTCCAATGG ACCCAAATGG ACTGTCTGAC CCGTACGTAA AGCTAAAGCT CATCCCAGAC 
CCAAAGAGCG AGAGCAAACA AAAGACCAAG ACTATCAAGT GTTCCCTGAA CCCAACCTGG 
AATGAGAGCT TTAAATTCCA GCTGAAGGAG TCCGATAAAG ATCGCAGGCT GTCCGTGGAA 
ATCTGGGACT GGGACTTAAC CAGTAGGAAT GATTTCATGG GGTCCCTGTC GTTTGGTATC 
TCGGAGCTAC TGAAGGCCGG CGTAGACGGT TGGTTCAAGT TGCTCAGCCA AGAGGAGGGA 
GAATACTTTA ACGTACCTGT GCCACCTGAA GGAGAAGAAG GCAACGAAGA GCTACGCCAG 
AAGTTTGAGA GAGCTAAAAT TGGCCCCGGG ACCAAAGCCG TTGAAGAGAA AGTGGTGAAC 
CCAATGCCCA AGGTGGACAA CAATGGAACC AGGGACCGTA TGAAAGTGTC AGACTTTAAC 
TTCCTAAAGG TTCTGGGGAA AGGCAGCTTT GGGAAGGTGA TCCTGGCAGA GAGGAAAGGA 
ACAGATGAGC TCTATGCAAT AAAGATCCTG AAGAAGGACG TGGTGATTCA GGATGATGAT 
GTTGAGTGCA CAATGGTAGA GAAGAGGGTC TTGGCTCTGT CTGGGAAGCC CCCATTCCTC 
ACTCAGCTGC ACTCCTGTTT CCAGACAATG GATCGCCTTT ACTTTGTCAT GGAGTACGTC 
AATGGTGGAG ACCTCATGTA CCAGATCCAG CAGGTCGGCC GATTCAAGGA ACCACATGCA 
GTATTCTACG CAGCAGAGAT AGCCATCGGC CTGCTATTCC TGCACAGCAA AGGCATCGTG 
TATAGGGACC TCAAGCTGGA TAACGTCATG CTGGATTCAG AGGGACACAT AAAGATCGCA 
GATTTCGGCA TGTGTAAGGA GAACATGTGG GACGGGGTAA CGACAAAGAC CTTCTGCGGC 
ACGCCAGACT ACATAGCGCC CGAGATTATA GCTTACCAGC CTTACGCCAA GTCAGTGGAT 
TGGTGGGCAT TCGGGATCCT TCTTTACGAG ATGTTAGCCG GGCAGGCTCC GTTCGAAGGG 
GAAGATGAGG ATGAGTTATT CCAGTCCATC ATGGAGCATA ATGTAGCATA TCCCAAATCA 
ATGTCCAAGG AAGCTGTGGC CATCTGTAAA GGGCTGATGA CAAAGCATCC TGGGAAGCGG 
CTGGGCTGTG GCCCAGAGGG GGAGCGAGAC ATTAAGGACC ATGCCTTTTT CCGATACATA 
GACTGGGAGA AGCTGGAAAG GAACGAGATC CAACCCCCCT ACAAGCCAAA GGCGAAGGAC 
AGGAAGGACA CCTCTAATTT TGACAAGGAG TTCACCCGCC AGCCCGTCGA GCTCACCCCC 
ACCGACAAAC TCTTTATCAT GAACCTGGAT CAAAATGAGT TTGCCGGATT CTCCTACGTC 
AACCCAGAGT TTGTGATCGT CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcb ( XM_012969929.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825383.1 Xenopus tropicalis protein kinase C beta (prkcb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa65788
Clone ID Related Accession (Same CDS sequence)	XM_012969929.3
Accession Version	XM_012969929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein kinase C beta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CCGCAGCCTG CGAGCCTGGG GAGGACACCA CCACCCGCTT TGCCAGGAAG 
GGGGCTCTGC GGCAGAAGAA TGTGCATGAG GTGAAGGAGC ACAAGTTCAC AGCCCGCTTC 
TTCAAGCAGC CCACCTTCTG CAGCCACTGC ACCGACTTCA TCTGGGGTTT TGGCAAACAG 
GGATTCCAGT GTCAAGTTTG CTGTTTCGTG GTGCACAAGC GGTGTCATGA GTTTGTCACG 
TTTTCCTGCC CGGGCGCAGA TAAAGGGCCA GCATCTGATG ACCCCCGCAG CAAACACAAG 
TTCAAAATTC ACACGTACTC CAGCCCGACG TTCTGTGACC ACTGCGGCTC ATTGCTGTAC 
GGCCTCATTC ACCAGGGGAT GAAATGTGAA ACCTGCATGA TGAATGTCCA CAAGCGCTGC 
GTAATGAACG TGCCAAGTCT GTGTGGCACC GACCACACCG AGCGAAGAGG AAGGATACAC 
ATCAAGGCGG AGATCCGCGA GGAAGTACTG ACAGTCACTG TCGGGGATGC GAGAAACCTA 
GTTCCAATGG ACCCAAATGG ACTGTCTGAC CCGTACGTAA AGCTAAAGCT CATCCCAGAC 
CCAAAGAGCG AGAGCAAACA AAAGACCAAG ACTATCAAGT GTTCCCTGAA CCCAACCTGG 
AATGAGAGCT TTAAATTCCA GCTGAAGGAG TCCGATAAAG ATCGCAGGCT GTCCGTGGAA 
ATCTGGGACT GGGACTTAAC CAGTAGGAAT GATTTCATGG GGTCCCTGTC GTTTGGTATC 
TCGGAGCTAC TGAAGGCCGG CGTAGACGGT TGGTTCAAGT TGCTCAGCCA AGAGGAGGGA 
GAATACTTTA ACGTACCTGT GCCACCTGAA GGAGAAGAAG GCAACGAAGA GCTACGCCAG 
AAGTTTGAGA GAGCTAAAAT TGGCCCCGGG ACCAAAGCCG TTGAAGAGAA AGTGGTGAAC 
CCAATGCCCA AGGTGGACAA CAATGGAACC AGGGACCGTA TGAAAGTGTC AGACTTTAAC 
TTCCTAAAGG TTCTGGGGAA AGGCAGCTTT GGGAAGGTGA TCCTGGCAGA GAGGAAAGGA 
ACAGATGAGC TCTATGCAAT AAAGATCCTG AAGAAGGACG TGGTGATTCA GGATGATGAT 
GTTGAGTGCA CAATGGTAGA GAAGAGGGTC TTGGCTCTGT CTGGGAAGCC CCCATTCCTC 
ACTCAGCTGC ACTCCTGTTT CCAGACAATG GATCGCCTTT ACTTTGTCAT GGAGTACGTC 
AATGGTGGAG ACCTCATGTA CCAGATCCAG CAGGTCGGCC GATTCAAGGA ACCACATGCA 
GTATTCTACG CAGCAGAGAT AGCCATCGGC CTGCTATTCC TGCACAGCAA AGGCATCGTG 
TATAGGGACC TCAAGCTGGA TAACGTCATG CTGGATTCAG AGGGACACAT AAAGATCGCA 
GACTTCGGCA TGTGTAAGGA GAACATGTGG GACGGGGTAA CGACAAAGAC CTTCTGCGGC 
ACGCCAGACT ACATAGCGCC CGAGATTATA GCTTACCAGC CTTACGCCAA GTCAGTGGAT 
TGGTGGGCAT TCGGGATCCT TCTTTACGAG ATGTTAGCCG GGCAGGCTCC GTTCGAAGGG 
GAAGATGAGG ATGAGTTATT CCAGTCCATC ATGGAGCATA ATGTAGCATA TCCCAAATCA 
ATGTCCAAGG AAGCTGTGGC CATCTGTAAA GGGCTGATGA CAAAGCATCC TGGGAAGCGG 
CTGGGCTGTG GCCCAGAGGG GGAGCGAGAC ATTAAGGACC ATGCCTTTTT CCGATACATA 
GACTGGGAGA AGCTGGAAAG GAACGAGATC CAACCCCCCT ACAAGCCAAA GGCGAAGGAC 
AGGAAGGACA CCTCTAATTT TGACAAGGAG TTCACCCGCC AGCCCGTCGA GCTCACCCCC 
ACCGACAAAC TCTTTATCAT GAACCTGGAT CAAAATGAGT TTGCCGGATT CTCCTACGTC 
AACCCAGAGT TTGTGATCGT CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825383.1
CDS	112..2118
Translation	MADPAACEPGEDTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLCGTDHTERRGRIHIKAEIREEVLTVTVGDARNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPTWNESFKFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELLKAGVDGWFKLLSQEEGEYFNVPVPPEGEEGNEELRQKFERAKIGPGTKAVEEKVVNPMPKVDNNGTRDRMKVSDFNFLKVLGKGSFGKVILAERKGTDELYAIKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYQIQQVGRFKEPHAVFYAAEIAIGLLFLHSKGIVYRDLKLDNVMLDSEGHIKIADFGMCKENMWDGVTTKTFCGTPDYIAPEIIAYQPYAKSVDWWAFGILLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKDHAFFRYIDWEKLERNEIQPPYKPKAKDRKDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYVNPEFVIVV

Target ORF information:

RefSeq Version	XM_012969929.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein kinase C beta (prkcb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969929.3

ORF Insert Sequence:

ATGGCTGACC CCGCAGCCTG CGAGCCTGGG GAGGACACCA CCACCCGCTT TGCCAGGAAG 
GGGGCTCTGC GGCAGAAGAA TGTGCATGAG GTGAAGGAGC ACAAGTTCAC AGCCCGCTTC 
TTCAAGCAGC CCACCTTCTG CAGCCACTGC ACCGACTTCA TCTGGGGTTT TGGCAAACAG 
GGATTCCAGT GTCAAGTTTG CTGTTTCGTG GTGCACAAGC GGTGTCATGA GTTTGTCACG 
TTTTCCTGCC CGGGCGCAGA TAAAGGGCCA GCATCTGATG ACCCCCGCAG CAAACACAAG 
TTCAAAATTC ACACGTACTC CAGCCCGACG TTCTGTGACC ACTGCGGCTC ATTGCTGTAC 
GGCCTCATTC ACCAGGGGAT GAAATGTGAA ACCTGCATGA TGAATGTCCA CAAGCGCTGC 
GTAATGAACG TGCCAAGTCT GTGTGGCACC GACCACACCG AGCGAAGAGG AAGGATACAC 
ATCAAGGCGG AGATCCGCGA GGAAGTACTG ACAGTCACTG TCGGGGATGC GAGAAACCTA 
GTTCCAATGG ACCCAAATGG ACTGTCTGAC CCGTACGTAA AGCTAAAGCT CATCCCAGAC 
CCAAAGAGCG AGAGCAAACA AAAGACCAAG ACTATCAAGT GTTCCCTGAA CCCAACCTGG 
AATGAGAGCT TTAAATTCCA GCTGAAGGAG TCCGATAAAG ATCGCAGGCT GTCCGTGGAA 
ATCTGGGACT GGGACTTAAC CAGTAGGAAT GATTTCATGG GGTCCCTGTC GTTTGGTATC 
TCGGAGCTAC TGAAGGCCGG CGTAGACGGT TGGTTCAAGT TGCTCAGCCA AGAGGAGGGA 
GAATACTTTA ACGTACCTGT GCCACCTGAA GGAGAAGAAG GCAACGAAGA GCTACGCCAG 
AAGTTTGAGA GAGCTAAAAT TGGCCCCGGG ACCAAAGCCG TTGAAGAGAA AGTGGTGAAC 
CCAATGCCCA AGGTGGACAA CAATGGAACC AGGGACCGTA TGAAAGTGTC AGACTTTAAC 
TTCCTAAAGG TTCTGGGGAA AGGCAGCTTT GGGAAGGTGA TCCTGGCAGA GAGGAAAGGA 
ACAGATGAGC TCTATGCAAT AAAGATCCTG AAGAAGGACG TGGTGATTCA GGATGATGAT 
GTTGAGTGCA CAATGGTAGA GAAGAGGGTC TTGGCTCTGT CTGGGAAGCC CCCATTCCTC 
ACTCAGCTGC ACTCCTGTTT CCAGACAATG GATCGCCTTT ACTTTGTCAT GGAGTACGTC 
AATGGTGGAG ACCTCATGTA CCAGATCCAG CAGGTCGGCC GATTCAAGGA ACCACATGCA 
GTATTCTACG CAGCAGAGAT AGCCATCGGC CTGCTATTCC TGCACAGCAA AGGCATCGTG 
TATAGGGACC TCAAGCTGGA TAACGTCATG CTGGATTCAG AGGGACACAT AAAGATCGCA 
GACTTCGGCA TGTGTAAGGA GAACATGTGG GACGGGGTAA CGACAAAGAC CTTCTGCGGC 
ACGCCAGACT ACATAGCGCC CGAGATTATA GCTTACCAGC CTTACGCCAA GTCAGTGGAT 
TGGTGGGCAT TCGGGATCCT TCTTTACGAG ATGTTAGCCG GGCAGGCTCC GTTCGAAGGG 
GAAGATGAGG ATGAGTTATT CCAGTCCATC ATGGAGCATA ATGTAGCATA TCCCAAATCA 
ATGTCCAAGG AAGCTGTGGC CATCTGTAAA GGGCTGATGA CAAAGCATCC TGGGAAGCGG 
CTGGGCTGTG GCCCAGAGGG GGAGCGAGAC ATTAAGGACC ATGCCTTTTT CCGATACATA 
GACTGGGAGA AGCTGGAAAG GAACGAGATC CAACCCCCCT ACAAGCCAAA GGCGAAGGAC 
AGGAAGGACA CCTCTAATTT TGACAAGGAG TTCACCCGCC AGCCCGTCGA GCTCACCCCC 
ACCGACAAAC TCTTTATCAT GAACCTGGAT CAAAATGAGT TTGCCGGATT CTCCTACGTC 
AACCCAGAGT TTGTGATCGT CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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prkcb cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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