Phox2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phox2a
Entrez Gene ID	116648
Full Name	paired-like homeobox 2a
Synonyms	Arix
General protein information	Preferred Names paired-like homeobox 2a Names paired mesoderm homeobox protein 2A ARIX1 homeodomain protein aristaless homeobox protein homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32
Summary	regulates transcription of the dopamine beta-hydroxylase and tyrosine hydroxylase genes, may play a role in neurotransmitter metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053869.1	NP_446321.1	paired mesoderm homeobox protein 2A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Phox2a	NP_446321.1
Danio rerio (zebrafish)	phox2a	NP_996953.1
Mus musculus (house mouse)	Phox2a	NP_032913.1
Xenopus tropicalis (tropical clawed frog)	phox2a	NP_001239128.1
Homo sapiens (human)	PHOX2A	NP_005160.2
Canis lupus familiaris (dog)	PHOX2A	XP_542326.2
Bos taurus (cattle)	PHOX2A	NP_001179086.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcription factor Phox2 upregulates expression of norepinephrine transporter and dopamine ?-hydroxylase in adult rat brains.
Fan Y, Huang J, Duffourc M, Kao RL, Ordway GA, Huang R, Zhu MY
Neuroscience19237-53(2011 Sep)

Specific and integrated roles of Lmx1a, Lmx1b and Phox2a in ventral midbrain development.
Deng Q, Andersson E, Hedlund E, Alekseenko Z, Coppola E, Panman L, Millonig JH, Brunet JF, Ericson J, Perlmann T
Development (Cambridge, England)138(16)3399-408(2011 Aug)

Ongoing roles of Phox2 homeodomain transcription factors during neuronal differentiation.
Coppola E, d'Autr?aux F, Rijli FM, Brunet JF
Development (Cambridge, England)137(24)4211-20(2010 Dec)

Distribution and phenotype of Phox2a-containing neurons in the adult sprague-dawley rat.
Card JP, Lois J, Sved AF
The Journal of comparative neurology518(12)2202-20(2010 Jun)

PHOX2A regulation of oculomotor complex nucleogenesis.
Hasan KB, Agarwala S, Ragsdale CW
Development (Cambridge, England)137(7)1205-13(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study demonstrates an upregulatory effect of Phox2a and Phox2b on the expression of dopamine beta-hydroxylase and norepinephrine transporter in noradrenergic neurons of rat brains
Title: Transcription factor Phox2 upregulates expression of norepinephrine transporter and dopamine ?-hydroxylase in adult rat brains.

mapped the distribution of Phox2a in the adult rat brain; data support the conclusion that Phox2a plays an important role in brainstem catecholamine neurotransmission and in the regulation of adaptive homeostatic functions in the adult nervous system
Title: Distribution and phenotype of Phox2a-containing neurons in the adult sprague-dawley rat.

Data show that exogenous Phox2a delivery to embryonic chick midbrain can drive a complete oculomotor complex molecular program, including the production of visceral and somatic motoneurons.
Title: PHOX2A regulation of oculomotor complex nucleogenesis.

data suggest that constitutive expression of Phox2a/2b defines the potential of neurons to become dopaminergic in response to membrane depolarization during a critical window of phenotypic plasticity.
Title: Expression of Phox2 transcription factors and induction of the dopaminergic phenotype in primary sensory neurons.

The following Phox2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phox2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10911	NM_053869.1 Latest version!	Rattus norvegicus paired-like homeobox 2a (Phox2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phox2a ( NM_053869.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446321.1 Rattus norvegicus paired-like homeobox 2a (Phox2a), mRNA.

Price & Availability↑

CloneID	ORa10911
Clone ID Related Accession (Same CDS sequence)	NM_053869.1
Accession Version	NM_053869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	paired mesoderm homeobox protein 2A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U25967.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U25967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00632253, SAMN02642595 [ECO:0000350] ##Evidence-Data-END##

ATGGACTACT CCTACCTCAA TTCGTACGAT TCGTGCGTGG CGGCCATGGA GGCGTCCGCC 
TACGGTGACT TCGGCGCCTG CAGCCAGCCT GGAGGCTTCC AATACAGTCC CCTGCGGCCT 
GCCTTTCCCG CCGCGGGGCC ACCGTGCCCC GCGCTCGGCT CCTCCAACTG TGCGCTTGGC 
GCCCTACGCG ACCACCAACC CGCACCCTAC TCGGCAGTTC CCTACAAGTT CTTCCCGGAG 
CCGTCCGGCC TGCATGAGAA GCGCAAGCAG CGGCGCATCC GCACCACGTT CACGAGTGCT 
CAGCTCAAGG AGTTGGAGCG CGTCTTCGCC GAGACCCACT ACCCGGACAT TTACACTCGT 
GAGGAACTGG CGCTCAAGAT CGACCTCACT GAGGCTCGCG TGCAGGTCTG GTTCCAGAAC 
CGCCGGGCTA AGTTCCGCAA ACAGGAGCGC GCGGCCACGG CCAAAGGCGC GGCGGGAGCG 
ACGGGCGCCA AAAAGGGCGA GGCGCGTTGC TCGTCGGAGG ACGACGACTC CAAGGAGTCC 
ACGTGCAGCC ACACGCCCGA CAGCACCGCG TCGCTGCCGC CGCCGCCGCC CGCACCCAGC 
CTGGCCAGCC CGCTCCTGAG CCCCAGCCCT CTGCCCGCCG CGCTGGGCTC CGGGCCCGGG 
CCCCAGCCAC TCAAAGGCGC GTTGTGGGCA GGGGTGGCGG GCGGTGGGGG TGGCGGGCCC 
GGCACGGGCG CAGCAGAGCT GCTTAAGGCC TGGCAGCCGG CGGAACCCGG ACCAGGTCCC 
TTCTCTGGAG TTCTGTCCTC CTTTCACCGG AAGCCCGGTC CCGCCCTGAA GACAAACCTC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446321.1
CDS	197..1042
Translation	MDYSYLNSYDSCVAAMEASAYGDFGACSQPGGFQYSPLRPAFPAAGPPCPALGSSNCALGALRDHQPAPYSAVPYKFFPEPSGLHEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAATAKGAAGATGAKKGEARCSSEDDDSKESTCSHTPDSTASLPPPPPAPSLASPLLSPSPLPAALGSGPGPQPLKGALWAGVAGGGGGGPGTGAAELLKAWQPAEPGPGPFSGVLSSFHRKPGPALKTNLF

Target ORF information:

RefSeq Version	NM_053869.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus paired-like homeobox 2a (Phox2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053869.1

ORF Insert Sequence:

ATGGACTACT CCTACCTCAA TTCGTACGAT TCGTGCGTGG CGGCCATGGA GGCGTCCGCC 
TACGGTGACT TCGGCGCCTG CAGCCAGCCT GGAGGCTTCC AATACAGTCC CCTGCGGCCT 
GCCTTTCCCG CCGCGGGGCC ACCGTGCCCC GCGCTCGGCT CCTCCAACTG TGCGCTTGGC 
GCCCTACGCG ACCACCAACC CGCACCCTAC TCGGCAGTTC CCTACAAGTT CTTCCCGGAG 
CCGTCCGGCC TGCATGAGAA GCGCAAGCAG CGGCGCATCC GCACCACGTT CACGAGTGCT 
CAGCTCAAGG AGTTGGAGCG CGTCTTCGCC GAGACCCACT ACCCGGACAT TTACACTCGT 
GAGGAACTGG CGCTCAAGAT CGACCTCACT GAGGCTCGCG TGCAGGTCTG GTTCCAGAAC 
CGCCGGGCTA AGTTCCGCAA ACAGGAGCGC GCGGCCACGG CCAAAGGCGC GGCGGGAGCG 
ACGGGCGCCA AAAAGGGCGA GGCGCGTTGC TCGTCGGAGG ACGACGACTC CAAGGAGTCC 
ACGTGCAGCC ACACGCCCGA CAGCACCGCG TCGCTGCCGC CGCCGCCGCC CGCACCCAGC 
CTGGCCAGCC CGCTCCTGAG CCCCAGCCCT CTGCCCGCCG CGCTGGGCTC CGGGCCCGGG 
CCCCAGCCAC TCAAAGGCGC GTTGTGGGCA GGGGTGGCGG GCGGTGGGGG TGGCGGGCCC 
GGCACGGGCG CAGCAGAGCT GCTTAAGGCC TGGCAGCCGG CGGAACCCGG ACCAGGTCCC 
TTCTCTGGAG TTCTGTCCTC CTTTCACCGG AAGCCCGGTC CCGCCCTGAA GACAAACCTC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription factor Phox2 upregulates expression of norepinephrine transporter and dopamine ?-hydroxylase in adult rat brains.
Neuroscience19237-53(2011 Sep)
Fan Y,Huang J,Duffourc M,Kao RL,Ordway GA,Huang R,Zhu MY

Specific and integrated roles of Lmx1a, Lmx1b and Phox2a in ventral midbrain development.
Development (Cambridge, England)138(16)3399-408(2011 Aug)
Deng Q,Andersson E,Hedlund E,Alekseenko Z,Coppola E,Panman L,Millonig JH,Brunet JF,Ericson J,Perlmann T

Ongoing roles of Phox2 homeodomain transcription factors during neuronal differentiation.
Development (Cambridge, England)137(24)4211-20(2010 Dec)
Coppola E,d'Autr?aux F,Rijli FM,Brunet JF

Distribution and phenotype of Phox2a-containing neurons in the adult sprague-dawley rat.
The Journal of comparative neurology518(12)2202-20(2010 Jun)
Card JP,Lois J,Sved AF

PHOX2A regulation of oculomotor complex nucleogenesis.
Development (Cambridge, England)137(7)1205-13(2010 Apr)
Hasan KB,Agarwala S,Ragsdale CW