GEMIN4 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GEMIN4
Entrez Gene ID	102456772
Full Name	gem nuclear organelle associated protein 4
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014581396.2	XP_014436882.1	LOW QUALITY PROTEIN: gem-associated protein 4
XM_014581396.1	XP_014436882.1	LOW QUALITY PROTEIN: gem-associated protein 4

Pathways from BioSystems
Gene Ontology

The following GEMIN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEMIN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe43421	XM_014581396.2 Latest version!	Pelodiscus sinensis gem nuclear organelle associated protein 4 (GEMIN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OPe43421	XM_014581396.1	Pelodiscus sinensis gem (nuclear organelle) associated protein 4 (GEMIN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GEMIN4 ( XM_014581396.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014436882.1 Pelodiscus sinensis gem nuclear organelle associated protein 4 (GEMIN4), mRNA.

Price & Availability↑

CloneID	OPe43421
Clone ID Related Accession (Same CDS sequence)	XM_014581396.2 , XM_014581396.1
Accession Version	XM_014581396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	LOW QUALITY PROTEIN: gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005871025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_014581396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

ATGACTCTGT GTGTGTGTCC TTCTCTTTCA ACAGGACCCT TGAACATCTG TGAGGAAACA 
GCCGTTCTGC ATGGCGGTTT CCTGCTAGCT GCGAAGTTGT ATCATCCTAA AGCGTTGACC 
GAGTTGGCCA AATCTGACTG GTCCTTGGTC GGGCCGCCCA TAACGGATGC CTTGAAGGAG 
ATATGCACCA GGTGTTCCTC TCCCCCAGCC CAACCCAATG CGTGGAAGAA GAAAGCTGTT 
ATCATCATCT GGGCCAAAAT CCTGCTCCCC TGCCCCTCGT CCTCAGTGGG CTCTTCCTCA 
GTTGACCAAC GATGGAAGGA AGATGTCTTC TTTTCGGTGG GCAGCATGAT CCCGCGTATC 
AACCGCACCA TCCTCTTCGA GCTGCTCAAG GCTCTGAATG CGCCGCGGCT CTTTGTGCAG 
CTGCTGCTGG CGTTGCCTGC AGCGGTGCGG CAGCGGGAGC TCGAGCTCTT TGTCAAGTAC 
GTCGCGAACG AGACGTCCCC GGTGGATGTC GCCTTCTTCC TGGACGTCTG GTGGGAGATA 
ATGAAACACA AGGAGGGTGA GCAAGACAAA ATGATCTTGA CGTTCGGCGC GCTGGTCCAT 
CAGCACCTGT CCGAGGCTCC GGACGAGTCC TGCCAGCCTG CAAAGAGATT CAAGGGGGAC 
CCCTTCTCCC AGCAGAGCTC CCACATGGCC CCCGGCCTGC TGATGGTTCT GGTTGATGGG 
CTAAAGCAGA TCTACGGGAG CATCGTCCTC CCCAAGATGA AGTGCTGTGC GCTGGCCAAC 
CTGGTGGAGC TGCTGTCGGT CTTCACAGTG ATCGAGCGCG AGTCCGGTTC GCTGCCCGTC 
AAAGCTTATC TAGACAAGAT TGCCTCTGTG GTCACCCTCT GGAACGACGA CAGCGAGAAC 
CGGTACAACA GGAGGAGTCT GGAGGAGAAA GTGAAGGAGG CGGAGAGGAG CGTCAGCTTG 
CTGTCCATGA TCAAGCTGTC CGGCGAAGAG CTGTTTGTTG GCTTGAGCTT CCTCCGTAGC 
TTGCTCCAGG GCTGGGGGGA GGAGCTGCCA GGCCTGCAGG AATTCGGAGA GCAGCGTCAT 
GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC CGGCACATGG AAACGGGAAG 
GACCTGATCG CCTTTGCCAA GTCTGGAGAC CTGTGTGAGG ACGAGAAGCA GATGGTGTTG 
GAACTGGGAC AGATCATCGT GGACTTCCTC AAGAAGATCC ACCCGAAGCT GAAAGGCAGG 
AATTCGGAGA GCAGCGTCAT GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC 
CGGCACATGG AAATGTGCTC CATTTTTGCT TCTGAGAAGG CTTGGGCCTT TTCTAAGGCC 
TGGGTCTCCT GTCTTGTTAA AAACAAAGCC CTCTTCCAGA AACCGGAGCT GGTCTTGAAA 
TTGCTGGAGA CCACCGTGAC GTTTAGCCTG TCCGCCGAGC ACAGGGACAG CAAGGAGGGG 
CAGAGTCAAG CGATCAGAAC CATTCTGGAA TGTTACACCG CGCTTCCGCT GCCCGACAAG 
AACAAAGTGA TCTCGGGCAT CCTGACTTCT TGGGGCGGGA AGGGCCTGTC CCTGAACTTG 
AAGGCTTTCA CGGAAGGGTT TCAAGAGGAG CTGAACTTGG CTTTCAACCA GATCACGCAG 
AGCGTGTCCG ATCAAGGTCT CACCAAGGCC GTCGCCTCGG TGGCCAGGCT GGTTCTGCTC 
TGCCCCGAGG CCACGGTGAA GAAAGTCTGC AACCTCGCGG TCGTCAACCT GGGAACGCAC 
CAGTTCCTAG CGCAGATCCT GTGCTCCTTT CCGGCCCTGA GGTTCCAAGA GACGCAAGAC 
GAGCCGAACA AGTCGGGCAG CCTGGTGGAG AGATGTCTGA AGGAGAGTGT CTGGGGGAAA 
CTCTCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT TGGCCTTTCT CATGCAGCCG 
AGCTCCGGTC AGCCTCTCCT CTCCCCTGCG GAGGTGACCA AAGCTTTTGT CTTTCCCCAT 
TTGAAATCAG ATTTTGCCCA CATTGAGCTG AGCTTGCAGA TCCTCAGTAA GGTTCTGGCA 
GTCCAGCCCA GTCCGGAGGA ACATCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT 
TGGCCTTTCT CATTGGCTCT TCTGGGCCTC TGCAAACTCC TGGATGGCTA CACCAAGTAC 
TGGCACCGGT TCAGGGACCA GCACTGCCCT GGGCTGGAGA CCAAAGATCT GATTGCCACC 
ACCCTCTCTC AGCTCTGTGA AGCGGTGGGG CAGAAAGCTG CCCCCTCTCC AGAAGTGTGG 
ATCCAGTCGT TGGCCTGGCT GCACCGGAAG GTCGAGACGC TGGACTGGAC CGTTGGTCTC 
CGGCTGAAAA AGCTTCTCGG GGACCACTTT AAGAACGAAG TCCCGGCGAC GCTGTTTGAA 
ATTTGCAAGC TCTCGGAGGA CGAGTGGACG TCCCGGCCCC TCCCGGCCTA CGGGGCGGGC 
AGCGGGCTGG GGGCGGGCAG CGGGCTGCTG GCCCAGATGG AGTGCTGCTG CATCTCCACC 
ACGCTGAGGG AGCAAATGCT TTCTCTCCTC GCCGTCAACA TGGACAACCC AGAAGAAGTC 
AACTTGTTCA GCAAGGGTTT CCTGGTGGCC CTGATCCAGG TCCTGCCATG GTGCAGCCAC 
AGCGAATGGA AGCAGCTCAC GCACGTGATT GACAGCCTGC TGCACCGGCA GGTCCTTCAC 
GTGCCTTACA CGCTGGAGTA CGTGCAGTAC ATGCCGCTGC TCAACTTCAG GCCCTTCGCC 
CGCCACCTCC AGTTCTCCGT GCTCTTGTTG AGAGGGTTCC AGTTGCTCTG CAGCTCTAGC 
TGCTCCAGCT GGCTGCCGGT GGAAGGCTGG CAGCATGTGG TGAGACTCTA CTGCAGCAGC 
CTCACCGACC TGCTGGGCTC GGTCCAGAGG GAGTCCCTCT CTCACCGGCA GTCTGACGAC 
GACAGGGACC CCACGCAGGA AGTGTCCTTC ATCTACATCC AGGTTTTCTG CCACGTGTTG 
CACGTGGCGG CCATGCTGCC AGATGACGGC ACCGGCGAGC CGCTCCTGGT CCTGGCCCTG 
GAGATCCTCT CGCAGTACAA AACGCTCAGC GCCTCTGACA AGTCCTTGAA CAGCACGCTC 
CGGAAGGCCA ACGAGAAGCA TTTCCTGGAG TCCATCACTG ACAACGTCCG CGACAAGGAG 
CTCCGGGCCA CGCTCCTGCA GAAGCTGAGC AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014436882.1
CDS	228..3506
Translation	MTLCVCPSLSTGPLNICEETAVLHGGFLLAAKLYHPKALTELAKSDWSLVGPPITDALKEICTRCSSPPAQPNAWKKKAVIIIWAKILLPCPSSSVGSSSVDQRWKEDVFFSVGSMIPRINRTILFELLKALNAPRLFVQLLLALPAAVRQRELELFVKYVANETSPVDVAFFLDVWWEIMKHKEGEQDKMILTFGALVHQHLSEAPDESCQPAKRFKGDPFSQQSSHMAPGLLMVLVDGLKQIYGSIVLPKMKCCALANLVELLSVFTVIERESGSLPVKAYLDKIASVVTLWNDDSENRYNRRSLEEKVKEAERSVSLLSMIKLSGEELFVGLSFLRSLLQGWGEELPGLQEFGEQRHGFGCHGYYEEDGPAHGNGKDLIAFAKSGDLCEDEKQMVLELGQIIVDFLKKIHPKLKGRNSESSVMALVAMAIIEKKMDRHMEMCSIFASEKAWAFSKAWVSCLVKNKALFQKPELVLKLLETTVTFSLSAEHRDSKEGQSQAIRTILECYTALPLPDKNKVISGILTSWGGKGLSLNLKAFTEGFQEELNLAFNQITQSVSDQGLTKAVASVARLVLLCPEATVKKVCNLAVVNLGTHQFLAQILCSFPALRFQETQDEPNKSGSLVERCLKESVWGKLSSTKEKEQFLEFLAFLMQPSSGQPLLSPAEVTKAFVFPHLKSDFAHIELSLQILSKVLAVQPSPEEHLLRRKRSSSSSWPFSLALLGLCKLLDGYTKYWHRFRDQHCPGLETKDLIATTLSQLCEAVGQKAAPSPEVWIQSLAWLHRKVETLDWTVGLRLKKLLGDHFKNEVPATLFEICKLSEDEWTSRPLPAYGAGSGLGAGSGLLAQMECCCISTTLREQMLSLLAVNMDNPEEVNLFSKGFLVALIQVLPWCSHSEWKQLTHVIDSLLHRQVLHVPYTLEYVQYMPLLNFRPFARHLQFSVLLLRGFQLLCSSSCSSWLPVEGWQHVVRLYCSSLTDLLGSVQRESLSHRQSDDDRDPTQEVSFIYIQVFCHVLHVAAMLPDDGTGEPLLVLALEILSQYKTLSASDKSLNSTLRKANEKHFLESITDNVRDKELRATLLQKLSKL

Target ORF information:

RefSeq Version	XM_014581396.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis gem nuclear organelle associated protein 4 (GEMIN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014581396.2

ORF Insert Sequence:

ATGACTCTGT GTGTGTGTCC TTCTCTTTCA ACAGGACCCT TGAACATCTG TGAGGAAACA 
GCCGTTCTGC ATGGCGGTTT CCTGCTAGCT GCGAAGTTGT ATCATCCTAA AGCGTTGACC 
GAGTTGGCCA AATCTGACTG GTCCTTGGTC GGGCCGCCCA TAACGGATGC CTTGAAGGAG 
ATATGCACCA GGTGTTCCTC TCCCCCAGCC CAACCCAATG CGTGGAAGAA GAAAGCTGTT 
ATCATCATCT GGGCCAAAAT CCTGCTCCCC TGCCCCTCGT CCTCAGTGGG CTCTTCCTCA 
GTTGACCAAC GATGGAAGGA AGATGTCTTC TTTTCGGTGG GCAGCATGAT CCCGCGTATC 
AACCGCACCA TCCTCTTCGA GCTGCTCAAG GCTCTGAATG CGCCGCGGCT CTTTGTGCAG 
CTGCTGCTGG CGTTGCCTGC AGCGGTGCGG CAGCGGGAGC TCGAGCTCTT TGTCAAGTAC 
GTCGCGAACG AGACGTCCCC GGTGGATGTC GCCTTCTTCC TGGACGTCTG GTGGGAGATA 
ATGAAACACA AGGAGGGTGA GCAAGACAAA ATGATCTTGA CGTTCGGCGC GCTGGTCCAT 
CAGCACCTGT CCGAGGCTCC GGACGAGTCC TGCCAGCCTG CAAAGAGATT CAAGGGGGAC 
CCCTTCTCCC AGCAGAGCTC CCACATGGCC CCCGGCCTGC TGATGGTTCT GGTTGATGGG 
CTAAAGCAGA TCTACGGGAG CATCGTCCTC CCCAAGATGA AGTGCTGTGC GCTGGCCAAC 
CTGGTGGAGC TGCTGTCGGT CTTCACAGTG ATCGAGCGCG AGTCCGGTTC GCTGCCCGTC 
AAAGCTTATC TAGACAAGAT TGCCTCTGTG GTCACCCTCT GGAACGACGA CAGCGAGAAC 
CGGTACAACA GGAGGAGTCT GGAGGAGAAA GTGAAGGAGG CGGAGAGGAG CGTCAGCTTG 
CTGTCCATGA TCAAGCTGTC CGGCGAAGAG CTGTTTGTTG GCTTGAGCTT CCTCCGTAGC 
TTGCTCCAGG GCTGGGGGGA GGAGCTGCCA GGCCTGCAGG AATTCGGAGA GCAGCGTCAT 
GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC CGGCACATGG AAACGGGAAG 
GACCTGATCG CCTTTGCCAA GTCTGGAGAC CTGTGTGAGG ACGAGAAGCA GATGGTGTTG 
GAACTGGGAC AGATCATCGT GGACTTCCTC AAGAAGATCC ACCCGAAGCT GAAAGGCAGG 
AATTCGGAGA GCAGCGTCAT GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC 
CGGCACATGG AAATGTGCTC CATTTTTGCT TCTGAGAAGG CTTGGGCCTT TTCTAAGGCC 
TGGGTCTCCT GTCTTGTTAA AAACAAAGCC CTCTTCCAGA AACCGGAGCT GGTCTTGAAA 
TTGCTGGAGA CCACCGTGAC GTTTAGCCTG TCCGCCGAGC ACAGGGACAG CAAGGAGGGG 
CAGAGTCAAG CGATCAGAAC CATTCTGGAA TGTTACACCG CGCTTCCGCT GCCCGACAAG 
AACAAAGTGA TCTCGGGCAT CCTGACTTCT TGGGGCGGGA AGGGCCTGTC CCTGAACTTG 
AAGGCTTTCA CGGAAGGGTT TCAAGAGGAG CTGAACTTGG CTTTCAACCA GATCACGCAG 
AGCGTGTCCG ATCAAGGTCT CACCAAGGCC GTCGCCTCGG TGGCCAGGCT GGTTCTGCTC 
TGCCCCGAGG CCACGGTGAA GAAAGTCTGC AACCTCGCGG TCGTCAACCT GGGAACGCAC 
CAGTTCCTAG CGCAGATCCT GTGCTCCTTT CCGGCCCTGA GGTTCCAAGA GACGCAAGAC 
GAGCCGAACA AGTCGGGCAG CCTGGTGGAG AGATGTCTGA AGGAGAGTGT CTGGGGGAAA 
CTCTCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT TGGCCTTTCT CATGCAGCCG 
AGCTCCGGTC AGCCTCTCCT CTCCCCTGCG GAGGTGACCA AAGCTTTTGT CTTTCCCCAT 
TTGAAATCAG ATTTTGCCCA CATTGAGCTG AGCTTGCAGA TCCTCAGTAA GGTTCTGGCA 
GTCCAGCCCA GTCCGGAGGA ACATCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT 
TGGCCTTTCT CATTGGCTCT TCTGGGCCTC TGCAAACTCC TGGATGGCTA CACCAAGTAC 
TGGCACCGGT TCAGGGACCA GCACTGCCCT GGGCTGGAGA CCAAAGATCT GATTGCCACC 
ACCCTCTCTC AGCTCTGTGA AGCGGTGGGG CAGAAAGCTG CCCCCTCTCC AGAAGTGTGG 
ATCCAGTCGT TGGCCTGGCT GCACCGGAAG GTCGAGACGC TGGACTGGAC CGTTGGTCTC 
CGGCTGAAAA AGCTTCTCGG GGACCACTTT AAGAACGAAG TCCCGGCGAC GCTGTTTGAA 
ATTTGCAAGC TCTCGGAGGA CGAGTGGACG TCCCGGCCCC TCCCGGCCTA CGGGGCGGGC 
AGCGGGCTGG GGGCGGGCAG CGGGCTGCTG GCCCAGATGG AGTGCTGCTG CATCTCCACC 
ACGCTGAGGG AGCAAATGCT TTCTCTCCTC GCCGTCAACA TGGACAACCC AGAAGAAGTC 
AACTTGTTCA GCAAGGGTTT CCTGGTGGCC CTGATCCAGG TCCTGCCATG GTGCAGCCAC 
AGCGAATGGA AGCAGCTCAC GCACGTGATT GACAGCCTGC TGCACCGGCA GGTCCTTCAC 
GTGCCTTACA CGCTGGAGTA CGTGCAGTAC ATGCCGCTGC TCAACTTCAG GCCCTTCGCC 
CGCCACCTCC AGTTCTCCGT GCTCTTGTTG AGAGGGTTCC AGTTGCTCTG CAGCTCTAGC 
TGCTCCAGCT GGCTGCCGGT GGAAGGCTGG CAGCATGTGG TGAGACTCTA CTGCAGCAGC 
CTCACCGACC TGCTGGGCTC GGTCCAGAGG GAGTCCCTCT CTCACCGGCA GTCTGACGAC 
GACAGGGACC CCACGCAGGA AGTGTCCTTC ATCTACATCC AGGTTTTCTG CCACGTGTTG 
CACGTGGCGG CCATGCTGCC AGATGACGGC ACCGGCGAGC CGCTCCTGGT CCTGGCCCTG 
GAGATCCTCT CGCAGTACAA AACGCTCAGC GCCTCTGACA AGTCCTTGAA CAGCACGCTC 
CGGAAGGCCA ACGAGAAGCA TTTCCTGGAG TCCATCACTG ACAACGTCCG CGACAAGGAG 
CTCCGGGCCA CGCTCCTGCA GAAGCTGAGC AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEMIN4 ( XM_014581396.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014436882.1 Pelodiscus sinensis gem (nuclear organelle) associated protein 4 (GEMIN4), mRNA.

Price & Availability↑

CloneID	OPe43421
Clone ID Related Accession (Same CDS sequence)	XM_014581396.2 , XM_014581396.1
Accession Version	XM_014581396.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	LOW QUALITY PROTEIN: gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005871025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

ATGACTCTGT GTGTGTGTCC TTCTCTTTCA ACAGGACCCT TGAACATCTG TGAGGAAACA 
GCCGTTCTGC ATGGCGGTTT CCTGCTAGCT GCGAAGTTGT ATCATCCTAA AGCGTTGACC 
GAGTTGGCCA AATCTGACTG GTCCTTGGTC GGGCCGCCCA TAACGGATGC CTTGAAGGAG 
ATATGCACCA GGTGTTCCTC TCCCCCAGCC CAACCCAATG CGTGGAAGAA GAAAGCTGTT 
ATCATCATCT GGGCCAAAAT CCTGCTCCCC TGCCCCTCGT CCTCAGTGGG CTCTTCCTCA 
GTTGACCAAC GATGGAAGGA AGATGTCTTC TTTTCGGTGG GCAGCATGAT CCCGCGTATC 
AACCGCACCA TCCTCTTCGA GCTGCTCAAG GCTCTGAATG CGCCGCGGCT CTTTGTGCAG 
CTGCTGCTGG CGTTGCCTGC AGCGGTGCGG CAGCGGGAGC TCGAGCTCTT TGTCAAGTAC 
GTCGCGAACG AGACGTCCCC GGTGGATGTC GCCTTCTTCC TGGACGTCTG GTGGGAGATA 
ATGAAACACA AGGAGGGTGA GCAAGACAAA ATGATCTTGA CGTTCGGCGC GCTGGTCCAT 
CAGCACCTGT CCGAGGCTCC GGACGAGTCC TGCCAGCCTG CAAAGAGATT CAAGGGGGAC 
CCCTTCTCCC AGCAGAGCTC CCACATGGCC CCCGGCCTGC TGATGGTTCT GGTTGATGGG 
CTAAAGCAGA TCTACGGGAG CATCGTCCTC CCCAAGATGA AGTGCTGTGC GCTGGCCAAC 
CTGGTGGAGC TGCTGTCGGT CTTCACAGTG ATCGAGCGCG AGTCCGGTTC GCTGCCCGTC 
AAAGCTTATC TAGACAAGAT TGCCTCTGTG GTCACCCTCT GGAACGACGA CAGCGAGAAC 
CGGTACAACA GGAGGAGTCT GGAGGAGAAA GTGAAGGAGG CGGAGAGGAG CGTCAGCTTG 
CTGTCCATGA TCAAGCTGTC CGGCGAAGAG CTGTTTGTTG GCTTGAGCTT CCTCCGTAGC 
TTGCTCCAGG GCTGGGGGGA GGAGCTGCCA GGCCTGCAGG AATTCGGAGA GCAGCGTCAT 
GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC CGGCACATGG AAACGGGAAG 
GACCTGATCG CCTTTGCCAA GTCTGGAGAC CTGTGTGAGG ACGAGAAGCA GATGGTGTTG 
GAACTGGGAC AGATCATCGT GGACTTCCTC AAGAAGATCC ACCCGAAGCT GAAAGGCAGG 
AATTCGGAGA GCAGCGTCAT GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC 
CGGCACATGG AAATGTGCTC CATTTTTGCT TCTGAGAAGG CTTGGGCCTT TTCTAAGGCC 
TGGGTCTCCT GTCTTGTTAA AAACAAAGCC CTCTTCCAGA AACCGGAGCT GGTCTTGAAA 
TTGCTGGAGA CCACCGTGAC GTTTAGCCTG TCCGCCGAGC ACAGGGACAG CAAGGAGGGG 
CAGAGTCAAG CGATCAGAAC CATTCTGGAA TGTTACACCG CGCTTCCGCT GCCCGACAAG 
AACAAAGTGA TCTCGGGCAT CCTGACTTCT TGGGGCGGGA AGGGCCTGTC CCTGAACTTG 
AAGGCTTTCA CGGAAGGGTT TCAAGAGGAG CTGAACTTGG CTTTCAACCA GATCACGCAG 
AGCGTGTCCG ATCAAGGTCT CACCAAGGCC GTCGCCTCGG TGGCCAGGCT GGTTCTGCTC 
TGCCCCGAGG CCACGGTGAA GAAAGTCTGC AACCTCGCGG TCGTCAACCT GGGAACGCAC 
CAGTTCCTAG CGCAGATCCT GTGCTCCTTT CCGGCCCTGA GGTTCCAAGA GACGCAAGAC 
GAGCCGAACA AGTCGGGCAG CCTGGTGGAG AGATGTCTGA AGGAGAGTGT CTGGGGGAAA 
CTCTCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT TGGCCTTTCT CATGCAGCCG 
AGCTCCGGTC AGCCTCTCCT CTCCCCTGCG GAGGTGACCA AAGCTTTTGT CTTTCCCCAT 
TTGAAATCAG ATTTTGCCCA CATTGAGCTG AGCTTGCAGA TCCTCAGTAA GGTTCTGGCA 
GTCCAGCCCA GTCCGGAGGA ACATCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT 
TGGCCTTTCT CATTGGCTCT TCTGGGCCTC TGCAAACTCC TGGATGGCTA CACCAAGTAC 
TGGCACCGGT TCAGGGACCA GCACTGCCCT GGGCTGGAGA CCAAAGATCT GATTGCCACC 
ACCCTCTCTC AGCTCTGTGA AGCGGTGGGG CAGAAAGCTG CCCCCTCTCC AGAAGTGTGG 
ATCCAGTCGT TGGCCTGGCT GCACCGGAAG GTCGAGACGC TGGACTGGAC CGTTGGTCTC 
CGGCTGAAAA AGCTTCTCGG GGACCACTTT AAGAACGAAG TCCCGGCGAC GCTGTTTGAA 
ATTTGCAAGC TCTCGGAGGA CGAGTGGACG TCCCGGCCCC TCCCGGCCTA CGGGGCGGGC 
AGCGGGCTGG GGGCGGGCAG CGGGCTGCTG GCCCAGATGG AGTGCTGCTG CATCTCCACC 
ACGCTGAGGG AGCAAATGCT TTCTCTCCTC GCCGTCAACA TGGACAACCC AGAAGAAGTC 
AACTTGTTCA GCAAGGGTTT CCTGGTGGCC CTGATCCAGG TCCTGCCATG GTGCAGCCAC 
AGCGAATGGA AGCAGCTCAC GCACGTGATT GACAGCCTGC TGCACCGGCA GGTCCTTCAC 
GTGCCTTACA CGCTGGAGTA CGTGCAGTAC ATGCCGCTGC TCAACTTCAG GCCCTTCGCC 
CGCCACCTCC AGTTCTCCGT GCTCTTGTTG AGAGGGTTCC AGTTGCTCTG CAGCTCTAGC 
TGCTCCAGCT GGCTGCCGGT GGAAGGCTGG CAGCATGTGG TGAGACTCTA CTGCAGCAGC 
CTCACCGACC TGCTGGGCTC GGTCCAGAGG GAGTCCCTCT CTCACCGGCA GTCTGACGAC 
GACAGGGACC CCACGCAGGA AGTGTCCTTC ATCTACATCC AGGTTTTCTG CCACGTGTTG 
CACGTGGCGG CCATGCTGCC AGATGACGGC ACCGGCGAGC CGCTCCTGGT CCTGGCCCTG 
GAGATCCTCT CGCAGTACAA AACGCTCAGC GCCTCTGACA AGTCCTTGAA CAGCACGCTC 
CGGAAGGCCA ACGAGAAGCA TTTCCTGGAG TCCATCACTG ACAACGTCCG CGACAAGGAG 
CTCCGGGCCA CGCTCCTGCA GAAGCTGAGC AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014436882.1
CDS	226..3504
Translation	MTLCVCPSLSTGPLNICEETAVLHGGFLLAAKLYHPKALTELAKSDWSLVGPPITDALKEICTRCSSPPAQPNAWKKKAVIIIWAKILLPCPSSSVGSSSVDQRWKEDVFFSVGSMIPRINRTILFELLKALNAPRLFVQLLLALPAAVRQRELELFVKYVANETSPVDVAFFLDVWWEIMKHKEGEQDKMILTFGALVHQHLSEAPDESCQPAKRFKGDPFSQQSSHMAPGLLMVLVDGLKQIYGSIVLPKMKCCALANLVELLSVFTVIERESGSLPVKAYLDKIASVVTLWNDDSENRYNRRSLEEKVKEAERSVSLLSMIKLSGEELFVGLSFLRSLLQGWGEELPGLQEFGEQRHGFGCHGYYEEDGPAHGNGKDLIAFAKSGDLCEDEKQMVLELGQIIVDFLKKIHPKLKGRNSESSVMALVAMAIIEKKMDRHMEMCSIFASEKAWAFSKAWVSCLVKNKALFQKPELVLKLLETTVTFSLSAEHRDSKEGQSQAIRTILECYTALPLPDKNKVISGILTSWGGKGLSLNLKAFTEGFQEELNLAFNQITQSVSDQGLTKAVASVARLVLLCPEATVKKVCNLAVVNLGTHQFLAQILCSFPALRFQETQDEPNKSGSLVERCLKESVWGKLSSTKEKEQFLEFLAFLMQPSSGQPLLSPAEVTKAFVFPHLKSDFAHIELSLQILSKVLAVQPSPEEHLLRRKRSSSSSWPFSLALLGLCKLLDGYTKYWHRFRDQHCPGLETKDLIATTLSQLCEAVGQKAAPSPEVWIQSLAWLHRKVETLDWTVGLRLKKLLGDHFKNEVPATLFEICKLSEDEWTSRPLPAYGAGSGLGAGSGLLAQMECCCISTTLREQMLSLLAVNMDNPEEVNLFSKGFLVALIQVLPWCSHSEWKQLTHVIDSLLHRQVLHVPYTLEYVQYMPLLNFRPFARHLQFSVLLLRGFQLLCSSSCSSWLPVEGWQHVVRLYCSSLTDLLGSVQRESLSHRQSDDDRDPTQEVSFIYIQVFCHVLHVAAMLPDDGTGEPLLVLALEILSQYKTLSASDKSLNSTLRKANEKHFLESITDNVRDKELRATLLQKLSKL

Target ORF information:

RefSeq Version	XM_014581396.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis gem (nuclear organelle) associated protein 4 (GEMIN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014581396.1

ORF Insert Sequence:

ATGACTCTGT GTGTGTGTCC TTCTCTTTCA ACAGGACCCT TGAACATCTG TGAGGAAACA 
GCCGTTCTGC ATGGCGGTTT CCTGCTAGCT GCGAAGTTGT ATCATCCTAA AGCGTTGACC 
GAGTTGGCCA AATCTGACTG GTCCTTGGTC GGGCCGCCCA TAACGGATGC CTTGAAGGAG 
ATATGCACCA GGTGTTCCTC TCCCCCAGCC CAACCCAATG CGTGGAAGAA GAAAGCTGTT 
ATCATCATCT GGGCCAAAAT CCTGCTCCCC TGCCCCTCGT CCTCAGTGGG CTCTTCCTCA 
GTTGACCAAC GATGGAAGGA AGATGTCTTC TTTTCGGTGG GCAGCATGAT CCCGCGTATC 
AACCGCACCA TCCTCTTCGA GCTGCTCAAG GCTCTGAATG CGCCGCGGCT CTTTGTGCAG 
CTGCTGCTGG CGTTGCCTGC AGCGGTGCGG CAGCGGGAGC TCGAGCTCTT TGTCAAGTAC 
GTCGCGAACG AGACGTCCCC GGTGGATGTC GCCTTCTTCC TGGACGTCTG GTGGGAGATA 
ATGAAACACA AGGAGGGTGA GCAAGACAAA ATGATCTTGA CGTTCGGCGC GCTGGTCCAT 
CAGCACCTGT CCGAGGCTCC GGACGAGTCC TGCCAGCCTG CAAAGAGATT CAAGGGGGAC 
CCCTTCTCCC AGCAGAGCTC CCACATGGCC CCCGGCCTGC TGATGGTTCT GGTTGATGGG 
CTAAAGCAGA TCTACGGGAG CATCGTCCTC CCCAAGATGA AGTGCTGTGC GCTGGCCAAC 
CTGGTGGAGC TGCTGTCGGT CTTCACAGTG ATCGAGCGCG AGTCCGGTTC GCTGCCCGTC 
AAAGCTTATC TAGACAAGAT TGCCTCTGTG GTCACCCTCT GGAACGACGA CAGCGAGAAC 
CGGTACAACA GGAGGAGTCT GGAGGAGAAA GTGAAGGAGG CGGAGAGGAG CGTCAGCTTG 
CTGTCCATGA TCAAGCTGTC CGGCGAAGAG CTGTTTGTTG GCTTGAGCTT CCTCCGTAGC 
TTGCTCCAGG GCTGGGGGGA GGAGCTGCCA GGCCTGCAGG AATTCGGAGA GCAGCGTCAT 
GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC CGGCACATGG AAACGGGAAG 
GACCTGATCG CCTTTGCCAA GTCTGGAGAC CTGTGTGAGG ACGAGAAGCA GATGGTGTTG 
GAACTGGGAC AGATCATCGT GGACTTCCTC AAGAAGATCC ACCCGAAGCT GAAAGGCAGG 
AATTCGGAGA GCAGCGTCAT GGCTTTGGTT GCCATGGCTA TTATTGAGAA GAAGATGGAC 
CGGCACATGG AAATGTGCTC CATTTTTGCT TCTGAGAAGG CTTGGGCCTT TTCTAAGGCC 
TGGGTCTCCT GTCTTGTTAA AAACAAAGCC CTCTTCCAGA AACCGGAGCT GGTCTTGAAA 
TTGCTGGAGA CCACCGTGAC GTTTAGCCTG TCCGCCGAGC ACAGGGACAG CAAGGAGGGG 
CAGAGTCAAG CGATCAGAAC CATTCTGGAA TGTTACACCG CGCTTCCGCT GCCCGACAAG 
AACAAAGTGA TCTCGGGCAT CCTGACTTCT TGGGGCGGGA AGGGCCTGTC CCTGAACTTG 
AAGGCTTTCA CGGAAGGGTT TCAAGAGGAG CTGAACTTGG CTTTCAACCA GATCACGCAG 
AGCGTGTCCG ATCAAGGTCT CACCAAGGCC GTCGCCTCGG TGGCCAGGCT GGTTCTGCTC 
TGCCCCGAGG CCACGGTGAA GAAAGTCTGC AACCTCGCGG TCGTCAACCT GGGAACGCAC 
CAGTTCCTAG CGCAGATCCT GTGCTCCTTT CCGGCCCTGA GGTTCCAAGA GACGCAAGAC 
GAGCCGAACA AGTCGGGCAG CCTGGTGGAG AGATGTCTGA AGGAGAGTGT CTGGGGGAAA 
CTCTCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT TGGCCTTTCT CATGCAGCCG 
AGCTCCGGTC AGCCTCTCCT CTCCCCTGCG GAGGTGACCA AAGCTTTTGT CTTTCCCCAT 
TTGAAATCAG ATTTTGCCCA CATTGAGCTG AGCTTGCAGA TCCTCAGTAA GGTTCTGGCA 
GTCCAGCCCA GTCCGGAGGA ACATCTTCTA CGAAGGAAAA GGAGCAGTTC CTAGAGTTCT 
TGGCCTTTCT CATTGGCTCT TCTGGGCCTC TGCAAACTCC TGGATGGCTA CACCAAGTAC 
TGGCACCGGT TCAGGGACCA GCACTGCCCT GGGCTGGAGA CCAAAGATCT GATTGCCACC 
ACCCTCTCTC AGCTCTGTGA AGCGGTGGGG CAGAAAGCTG CCCCCTCTCC AGAAGTGTGG 
ATCCAGTCGT TGGCCTGGCT GCACCGGAAG GTCGAGACGC TGGACTGGAC CGTTGGTCTC 
CGGCTGAAAA AGCTTCTCGG GGACCACTTT AAGAACGAAG TCCCGGCGAC GCTGTTTGAA 
ATTTGCAAGC TCTCGGAGGA CGAGTGGACG TCCCGGCCCC TCCCGGCCTA CGGGGCGGGC 
AGCGGGCTGG GGGCGGGCAG CGGGCTGCTG GCCCAGATGG AGTGCTGCTG CATCTCCACC 
ACGCTGAGGG AGCAAATGCT TTCTCTCCTC GCCGTCAACA TGGACAACCC AGAAGAAGTC 
AACTTGTTCA GCAAGGGTTT CCTGGTGGCC CTGATCCAGG TCCTGCCATG GTGCAGCCAC 
AGCGAATGGA AGCAGCTCAC GCACGTGATT GACAGCCTGC TGCACCGGCA GGTCCTTCAC 
GTGCCTTACA CGCTGGAGTA CGTGCAGTAC ATGCCGCTGC TCAACTTCAG GCCCTTCGCC 
CGCCACCTCC AGTTCTCCGT GCTCTTGTTG AGAGGGTTCC AGTTGCTCTG CAGCTCTAGC 
TGCTCCAGCT GGCTGCCGGT GGAAGGCTGG CAGCATGTGG TGAGACTCTA CTGCAGCAGC 
CTCACCGACC TGCTGGGCTC GGTCCAGAGG GAGTCCCTCT CTCACCGGCA GTCTGACGAC 
GACAGGGACC CCACGCAGGA AGTGTCCTTC ATCTACATCC AGGTTTTCTG CCACGTGTTG 
CACGTGGCGG CCATGCTGCC AGATGACGGC ACCGGCGAGC CGCTCCTGGT CCTGGCCCTG 
GAGATCCTCT CGCAGTACAA AACGCTCAGC GCCTCTGACA AGTCCTTGAA CAGCACGCTC 
CGGAAGGCCA ACGAGAAGCA TTTCCTGGAG TCCATCACTG ACAACGTCCG CGACAAGGAG 
CTCCGGGCCA CGCTCCTGCA GAAGCTGAGC AAGCTGTGA