NCU08365 cDNA ORF clone, Neurospora crassa OR74A

The following NCU08365 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08365 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf08553 XM_960175.2
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Neurospora crassa OR74A RNA polymerase II mediator complex component Med8 (NCU08365), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf08553
    Clone ID Related Accession (Same CDS sequence) XM_960175.2
    Accession Version XM_960175.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product RNA polymerase II mediator complex component Med8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960175.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCCGATG GCAAAGATTC CCTCCGTCTA GCTCCCGATG AATTCAAGCA GCTCGAGGTT 
    CTCCGAAACC GATTCCATCA ACTTACTCAC AGTTTGAACA GCCTGTTTAA TGATATCCAG
    CGAAGCCAGC CTCTCCCAAG TCAGGAGTCC CTCACAGCCT CAGCAGCCAT TCTCCAAAAG
    AGTCTCGAAA CTATCCAAAA GCTCGCAGAC GAAAACTCCG ACTTGTTCCA ACGCATCGTC
    GTCCACCCAT CGACAAACTT CCCTGGGCGC ACTCAGGAAC ACATCCTTCT GCAACTACTC
    AGAAAGAAAC TCGAGCCGGA AGTCGAGAGC TGGGTCGAAG ATGCGCGTAG CGTAGCAAAG
    GCCTCTGGTT TGGACCCGAG CAAACTGGCC AGCTCCCAAC AGAGGAGCAA CGACTACGAC
    GATGACGACG ACGACTACCG AGACGAAGAG GAGGACGCGC CCTCGGACCC GTTCAACGAA
    CAGTGGGCCG ATATGTTTCA GGTCAGCATG GACAGCATCA GGGATTACCT CATGAACCAA
    GCCAACGAGC CCTATACGAT CGCCGAGCGC GAGATGGGCA TTGAGAATGT GCGCACCGGT
    CTGAAGCGAT CACTTGAGGA GGAGGACAGC GAGGAAGAAG ACGAGGACGA GGACGAGGAC
    GAAGACAGAT CGCTTGCTAC CGCTGGGCTC GGCAACATCA ATGCGGCGAA TCTGTTCCAT
    CCAGAGCATG TCCTCTGGCT TGCTTCGAGG GGCAATATGA AGGTCCCGAA GAATGTTGAC
    CTGGAGGCCA TACGCAAGAG AGAGAAACCT AGGGGGCGTC CGGCTCCCGC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_965268.1
    CDS170..1006
    Translation

    Target ORF information:

    RefSeq Version XM_960175.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A RNA polymerase II mediator complex component Med8 (NCU08365), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_960175.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCCGATG GCAAAGATTC CCTCCGTCTA GCTCCCGATG AATTCAAGCA GCTCGAGGTT 
    CTCCGAAACC GATTCCATCA ACTTACTCAC AGTTTGAACA GCCTGTTTAA TGATATCCAG
    CGAAGCCAGC CTCTCCCAAG TCAGGAGTCC CTCACAGCCT CAGCAGCCAT TCTCCAAAAG
    AGTCTCGAAA CTATCCAAAA GCTCGCAGAC GAAAACTCCG ACTTGTTCCA ACGCATCGTC
    GTCCACCCAT CGACAAACTT CCCTGGGCGC ACTCAGGAAC ACATCCTTCT GCAACTACTC
    AGAAAGAAAC TCGAGCCGGA AGTCGAGAGC TGGGTCGAAG ATGCGCGTAG CGTAGCAAAG
    GCCTCTGGTT TGGACCCGAG CAAACTGGCC AGCTCCCAAC AGAGGAGCAA CGACTACGAC
    GATGACGACG ACGACTACCG AGACGAAGAG GAGGACGCGC CCTCGGACCC GTTCAACGAA
    CAGTGGGCCG ATATGTTTCA GGTCAGCATG GACAGCATCA GGGATTACCT CATGAACCAA
    GCCAACGAGC CCTATACGAT CGCCGAGCGC GAGATGGGCA TTGAGAATGT GCGCACCGGT
    CTGAAGCGAT CACTTGAGGA GGAGGACAGC GAGGAAGAAG ACGAGGACGA GGACGAGGAC
    GAAGACAGAT CGCTTGCTAC CGCTGGGCTC GGCAACATCA ATGCGGCGAA TCTGTTCCAT
    CCAGAGCATG TCCTCTGGCT TGCTTCGAGG GGCAATATGA AGGTCCCGAA GAATGTTGAC
    CTGGAGGCCA TACGCAAGAG AGAGAAACCT AGGGGGCGTC CGGCTCCCGC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B