sad-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	sad-1
Entrez Gene ID	3880397
Full Name	suppressor of ascus-dominance-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959155.3	XP_964248.3	RNA dependent RNA polymerase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rdp1	NP_593295.1
Neurospora crassa	NCU02178	XP_964248.2
Arabidopsis thaliana (thale cress)	RDR1	NP_172932.1
Arabidopsis thaliana (thale cress)	RDR6	NP_190519.1
Oryza sativa (rice)	Os02g0736200	NP_001048051.1
Magnaporthe oryzae (rice blast fungus)	MGG_02748	XP_003721007.1
Arabidopsis thaliana (thale cress)	RDR2	NP_192851.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sad-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sad-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12747	XM_959155.3 Latest version!	Neurospora crassa OR74A RNA dependent RNA polymerase (sad-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sad-1 ( XM_959155.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964248.3 Neurospora crassa OR74A RNA dependent RNA polymerase (sad-1), partial mRNA.

Price & Availability↑

CloneID	ONf12747
Clone ID Related Accession (Same CDS sequence)	XM_959155.3
Accession Version	XM_959155.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	RNA dependent RNA polymerase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959155.2. COMPLETENESS: incomplete on both ends.

ATGTCAGGAA TACCCAACTG GGTAGAGCAG GCGCAGCATG CCATTCTTGT GTCGCCGGAC 
AGAAGCCCCA AAGATCAGGA CGCTCGTCCG AGGCATCCTA GCCGCCGCAG ATCCAGCCAG 
CAGGGACATC GAGAGAACAC ACCGACACCT CGACCACCCT CGCGACCAAC CTCGCGACCC 
TCCTCCAGCT CTCCCAAGCC TCCCCCTCGC GCTTTTCGGC AGTTCTCACG GGGCAAACCA 
GCCTCATTCG CATCCCTCCC CTCTCTGACA CAACCAGTCT TCATTTCCAG GGAGTGGAAG 
TGCGCCAGGA CCGCTGCCAT TCGCGTCAAG GATGTGCCGC CTAGAGCTAA ACTCCAGGAC 
CTTCACGATC TCTTCTCTGA GTATGGTCAT ATTAGCTACA TTGAACTTGA TGAGGACCGT 
CAAGATGTTT CTGATCGTGG CCGGAGAGCT CTGATTCGCT TTGAGCCACC TCCGCTATCT 
ACCGACTTTC TCTGCCGAGG CATCTGCAGA CTCAGGATTG GACTTGTTGA CCACTGGCTG 
CCTCTCGAGA CAGTGGACCC AAAGCGCGAG GATCGCAAAG AACGTACCAT CAAGACTCAG 
CTCGGCAACA CTTGCCCGCA AACTCTATAC GTCTTTCCCT CAACACTGTC TTTCGGTCTC 
TTAGTCCAGC CTGCGGTATT CATGAAGAAA CAATCCGTTC AAACCCTCTC GTCCGACTCT 
TTCCTGAGAC TTGAGTTCGA TTTCAAGCGC ATGCGACTGG TAATTAAATT CTCCCTCCAC 
TACCAACGCG AACTTAGTGG GTTTCGCAGA CAAAATGAGC ATTACAAGCT CCACATTAAG 
TTTGGAGTCA TCAAAGAGTT ATGCAGAACC ATGGTCGGCG AGGAGCATCG TCAAGCTTTG 
GTCATAACTC TCCGGGACCC GCCGGTTGCG TACAGGAAGA GGGATGTCTC AAAGACATTT 
GGCGAGGATC GGCTGACTTG GAGCGAGAAT GACCTCTGGG AGAGAGTGGT CGACATTAGC 
CCTGGACTCG ATGTCAGCAA AAACCCAGTT TCGCTCGCCG AGAACCACCA ATATATTGAC 
CTGGGACGGT GGCTTACCTA TTGGATTGAA CTGGATCAAC AATCAACAAG AGTCTGGGAT 
CAAGTTCAAC AATATTTGCT CGATTGGAAT CTCAGGACTA AGTTGACTGT TTTCCCCGAG 
CCGCTTCCCA ACCAAAAACC AAAGGTATGG GATTTTCTGG ATGATAGATA CGGCCATGAC 
ATTCAACAAG TCTCCTCACG GTCCTGGAGC AACGACTTTT CTCTTTTAGC CGCGCCGCCC 
CGTATTTCTC TACCGTTTGA TGTTCGGTAT CAGCTTGAAG TCTGCATATC GCAAGGAATC 
ATCAACGAGC ACAACATTGA CCGGCCGTTC CTTGAGAAGC TGATGGAATT TTGTGATAAT 
GACAACAGCT TCGGCAAAGA TCGGGCTCGC CTCATCCTCG AATACGTCGC CGATGAGTAT 
GCTGGAAAGC GTATCTTCGA CCCGATGGAA CTCTTCAAGG ATCATGCAGC CTTAGCTTAT 
TTCCCAACTA GTTTCATGAT ACCCAACCAT TGCGCTTGGG TTCGTAGGGT CACCATCACC 
CCTACTCGTA TCTACTTCAG TACCCCCTGC GTCGAACCCA CGAACCGCGT CATCCGCCAG 
TGGAAACATG CACAAGACTA CTTTATCCGC ATTCAATTCA CCGATGAAGT TCTTGAAGGA 
CGTATCAAGA GCGGCGAAGC GGAACTTCCC CTATTCTTGC GTGTGTACAG GGTATTGGAG 
AAAGGGGTTG CCATGGGTCC GTGGCATTGG AAGTTCCTTG CATTTGGCAA CTCTCAGATT 
CGAGAAGCCG GAGCCTTCAT GTTCTGTGAA CAGAGCAATT TGACAGGCGA TATGATGCGA 
GCCTGGATGG GCCGCTTCAG TCACATCAAG GTCATTGCCA AGTATGCGGC TAGACTTGGG 
CAGTGTTTCT CAACTACCCG TTTGGTGCCT GGCATCCCGG CACCGAGGAT CGTCACCATC 
CCTGATGTCG AAAAGGACGG ATTTTGCTTC ACGGATGGAG TAGGCAAGAT TTCCCCTTTG 
CTTGCAAAGA TCGTAGCGCA TGACTGGAGC ATTGACCCGC CGCCGTCAGC TTACCAGTTC 
CGCATGGGAG GTTGCAAGGG AGTCTTGGTA ACTTGGCCTG ATGTGAAGGG TATGGAGGTT 
CATATTCGGA AGTCGCAGGA AAAGTTCGTT GCCGAATTCA ATGGCCTTGA GGTTGTCAGA 
TGCTCGCAGT TCTCAACCGC CACTCTCAAC CGGCAAATCA TTGCCGTTCT CTCGTCTCTT 
GGTGTTCCCG ATCAAGTCTT TGTAGACATG ATGGAGCAGC AGCTTTCTGA CTTCAATGCG 
GCGATGGAGG ACAAACAAAA GGCGACGGCG ATTCTGAAAA CGTTCATTGA TGAAAATCAT 
ATGACACCTA TCATTGCGGA GATGCTTGCC TACGGCTTTA TGGGGTCTCA GGAGCCTTTT 
GTTCGCACCC TACTTCAGCT GTGGCGATCA TGGTCGATCA AAACGTTGAA GGAGAAAGCG 
CGACTCAATG TCGAAAAGAG TGCGTTCGTG CTCGGCTGTG TTGATGAGAC TGGCACTCTG 
AAAGGGCACA TGAAGGTCAT TGAAGACTGG AAAGACGTGT CCAGTGAGAA GCTTCCACAG 
ATCTTCCTAC AGATTCCCGA TGATGTCAAT GGCGGGTACA GGGTTATTAC TGGAACCTGC 
GTCGTTGGCA GAAACCCGTC TCTCCATCCT GGTGATATCC GTGTGGTGGA GGCAGTTGAT 
GTCCCTGCTT TGCGGCATCT AAGAGACGTG GTGGTGTTCC CCTTGACTGG GGACAGAGAC 
GTGCCCAGCA TGTGCTCTGG TGGAGACCTC GATGGTGATG ACTTCTTTGT TATTTGGGAT 
CCTTTGCTTA TCCCGAAAGA ACGCTCCCAT CCCCCAATGA TCAGTGAGCC CATTGCGGGC 
AAGGAGTTGG CAACCGAACC GACCGTAAAC AACCTGATTA CCTTCTTTGT GTTGTATATG 
AAGTACAACA ATCTGCCGTT GATCGCTCAT GCTCATCTGG CGACAGCAGA TGCGGAGGTG 
GAAGGAGTGA AGAGCCCGAA ATGCCTCGAG CTCGCTTCAC TTCACTCCAT GGCTGTTGAC 
TACGTGAAGA CTGGTGTTGC CGCCGAGTTT CCGAGACGGC TTGACCCCAA GACCTGGCCT 
CATTTCATGG AGAAAAACAG ACACACTTAC CACTCAGTAA CAGCACTTGG AAAGCTCTAC 
GATATGGTTA AACGGGAAAC ATTCGACATG AAAGAGAACT ACCAGCTGCC GTTTGATAAC 
CGCATCCTCA AGCACACAAA GTGCCGCGCT CTTAGGGATG GGACGCTGGC CAAAGCCCGT 
CGCATCAAAT CTCAGTACGA CACCGCTATG CGCCGCGTGA TGTGCCAGCT CGAAATTGCG 
ACCGAGTTCG AAGTTTGGAC CGCCTTTGTC ATGTCCAAAC CCCGCGTGGG CTCAGACTAC 
AAACTCCAAG ACAACGTCGG TCGCGAGTCT TCTGCCCTCA AGCAACACTT CAAGGATCAG 
TGTAAAAAGG AGGCTGGGGG TGATCTCCTT TCCTTCGTAT CGGCCATGTA TAGAGTCACT 
TACGAAGAAG TTCGCATTGC CCTTTTCGAA GCCAAGCAGC CGCACGTTAG ACCTGACGGC 
AGGCTCGGAA CCAGAAAAAT TACACCAAAG ACCATGCCAC TTGTTAGCTT TCCCTGGCTA 
TTTTGGGACA AGCTCGGTGA GCTTGCCAGG GCAGGCGCCG TATTGCAAAG GAGACTTGAC 
GATGGAAGTG AGGATATGGA CCTTTTGAGC GACGTCCCGC TCGTTTCCCA GAGGCGCAGA 
GGCAAACATA ATGCAAGTGG TAGCAGTGAC TTTATGGATG AGCATGGTGA TCCCCTTTCC 
TATACACGCA CAAGCGATGG CAAGATCATT CACTATGGTC AGATCTTGAA CCTTTTCAGT 
CACGACGATG AAGATGGAGA TGAGAGGAAT GACGCCCGGG ACAGAAACAG TTCCAGCGAA 
GATTCTACTC ATGTTTCTAA TTCGTCCAAA TCCAGCTCCA ACCTTAGCCC GGTTGCTGAG 
GAGGACCTTC TTACTTTTGA GTCGCCTCCA GCTAGTCCAG GTGTCACTCC TGCGTCTCCG 
CAAGTTGATC TCCTAGGCCC GACACTTACG CTCACTAAGG CCGAGCTCGC CATCGGTCTC 
GCTGGTCCGG TTGCCACTAC CTATACTCCT CCGCCGACGG ATGAGGAGAC AGTAATTTGC 
CATGGTCACT CCAACCGCGC CAGTCACCTC AGTGCTTCGT CTTCGTCACT TGATCCTCAA 
CCTCTGATTG AGGAGACTTC CGGGGAGAAC GAGGATCTCC TGCTAGATAT TTATTCTGCG 
TCTCCCCCTA GGGCGGATGG CGCTAGATTA GCCTCGGCCG TTGGAAGTGA TGTTCCTGTC 
AAGGGCGGGC CTCCTCCCGT TTGGATGGAG CAGGTGATCA GAATGGGCCA CAGGATTCCA 
ACCCCTCCTC TTGACTCAAT TGCGATCCCG AATGTGATTG GGGTTCCGCA ATTCGAGAGT 
CCGCTGACTG GGACTGTTGT GGCTTCTGCT ATTCAGGATC CGTTTGTTTC CCCTTCTCCT 
GCGGTTGCCA CTCCTGCTAC CAGTGGTAGT GGTGGTGGTT GGGGTGGTGG TGGTGGTGGT 
GGTGGTGGTG GATATATCGG TATTGGCGGT CCCATCAACA AGGAGCTCGT CATGGGATTG 
GACGGAGGTA TCAAAGAGGA AGATCGCGAG GAAACCGACG AGGAGGCTGA GGAAGTCGAG 
TTGGAGATTG ACGAGGACCC AATTGCAGTG CGTTATGCAC AGATGGCGGC GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964248.3
CDS	1..4917
Translation	MSGIPNWVEQAQHAILVSPDRSPKDQDARPRHPSRRRSSQQGHRENTPTPRPPSRPTSRPSSSSPKPPPRAFRQFSRGKPASFASLPSLTQPVFISREWKCARTAAIRVKDVPPRAKLQDLHDLFSEYGHISYIELDEDRQDVSDRGRRALIRFEPPPLSTDFLCRGICRLRIGLVDHWLPLETVDPKREDRKERTIKTQLGNTCPQTLYVFPSTLSFGLLVQPAVFMKKQSVQTLSSDSFLRLEFDFKRMRLVIKFSLHYQRELSGFRRQNEHYKLHIKFGVIKELCRTMVGEEHRQALVITLRDPPVAYRKRDVSKTFGEDRLTWSENDLWERVVDISPGLDVSKNPVSLAENHQYIDLGRWLTYWIELDQQSTRVWDQVQQYLLDWNLRTKLTVFPEPLPNQKPKVWDFLDDRYGHDIQQVSSRSWSNDFSLLAAPPRISLPFDVRYQLEVCISQGIINEHNIDRPFLEKLMEFCDNDNSFGKDRARLILEYVADEYAGKRIFDPMELFKDHAALAYFPTSFMIPNHCAWVRRVTITPTRIYFSTPCVEPTNRVIRQWKHAQDYFIRIQFTDEVLEGRIKSGEAELPLFLRVYRVLEKGVAMGPWHWKFLAFGNSQIREAGAFMFCEQSNLTGDMMRAWMGRFSHIKVIAKYAARLGQCFSTTRLVPGIPAPRIVTIPDVEKDGFCFTDGVGKISPLLAKIVAHDWSIDPPPSAYQFRMGGCKGVLVTWPDVKGMEVHIRKSQEKFVAEFNGLEVVRCSQFSTATLNRQIIAVLSSLGVPDQVFVDMMEQQLSDFNAAMEDKQKATAILKTFIDENHMTPIIAEMLAYGFMGSQEPFVRTLLQLWRSWSIKTLKEKARLNVEKSAFVLGCVDETGTLKGHMKVIEDWKDVSSEKLPQIFLQIPDDVNGGYRVITGTCVVGRNPSLHPGDIRVVEAVDVPALRHLRDVVVFPLTGDRDVPSMCSGGDLDGDDFFVIWDPLLIPKERSHPPMISEPIAGKELATEPTVNNLITFFVLYMKYNNLPLIAHAHLATADAEVEGVKSPKCLELASLHSMAVDYVKTGVAAEFPRRLDPKTWPHFMEKNRHTYHSVTALGKLYDMVKRETFDMKENYQLPFDNRILKHTKCRALRDGTLAKARRIKSQYDTAMRRVMCQLEIATEFEVWTAFVMSKPRVGSDYKLQDNVGRESSALKQHFKDQCKKEAGGDLLSFVSAMYRVTYEEVRIALFEAKQPHVRPDGRLGTRKITPKTMPLVSFPWLFWDKLGELARAGAVLQRRLDDGSEDMDLLSDVPLVSQRRRGKHNASGSSDFMDEHGDPLSYTRTSDGKIIHYGQILNLFSHDDEDGDERNDARDRNSSSEDSTHVSNSSKSSSNLSPVAEEDLLTFESPPASPGVTPASPQVDLLGPTLTLTKAELAIGLAGPVATTYTPPPTDEETVICHGHSNRASHLSASSSSLDPQPLIEETSGENEDLLLDIYSASPPRADGARLASAVGSDVPVKGGPPPVWMEQVIRMGHRIPTPPLDSIAIPNVIGVPQFESPLTGTVVASAIQDPFVSPSPAVATPATSGSGGGWGGGGGGGGGGYIGIGGPINKELVMGLDGGIKEEDREETDEEAEEVELEIDEDPIAVRYAQMAAL

Target ORF information:

RefSeq Version	XM_959155.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A RNA dependent RNA polymerase (sad-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959155.3

ORF Insert Sequence:

ATGTCAGGAA TACCCAACTG GGTAGAGCAG GCGCAGCATG CCATTCTTGT GTCGCCGGAC 
AGAAGCCCCA AAGATCAGGA CGCTCGTCCG AGGCATCCTA GCCGCCGCAG ATCCAGCCAG 
CAGGGACATC GAGAGAACAC ACCGACACCT CGACCACCCT CGCGACCAAC CTCGCGACCC 
TCCTCCAGCT CTCCCAAGCC TCCCCCTCGC GCTTTTCGGC AGTTCTCACG GGGCAAACCA 
GCCTCATTCG CATCCCTCCC CTCTCTGACA CAACCAGTCT TCATTTCCAG GGAGTGGAAG 
TGCGCCAGGA CCGCTGCCAT TCGCGTCAAG GATGTGCCGC CTAGAGCTAA ACTCCAGGAC 
CTTCACGATC TCTTCTCTGA GTATGGTCAT ATTAGCTACA TTGAACTTGA TGAGGACCGT 
CAAGATGTTT CTGATCGTGG CCGGAGAGCT CTGATTCGCT TTGAGCCACC TCCGCTATCT 
ACCGACTTTC TCTGCCGAGG CATCTGCAGA CTCAGGATTG GACTTGTTGA CCACTGGCTG 
CCTCTCGAGA CAGTGGACCC AAAGCGCGAG GATCGCAAAG AACGTACCAT CAAGACTCAG 
CTCGGCAACA CTTGCCCGCA AACTCTATAC GTCTTTCCCT CAACACTGTC TTTCGGTCTC 
TTAGTCCAGC CTGCGGTATT CATGAAGAAA CAATCCGTTC AAACCCTCTC GTCCGACTCT 
TTCCTGAGAC TTGAGTTCGA TTTCAAGCGC ATGCGACTGG TAATTAAATT CTCCCTCCAC 
TACCAACGCG AACTTAGTGG GTTTCGCAGA CAAAATGAGC ATTACAAGCT CCACATTAAG 
TTTGGAGTCA TCAAAGAGTT ATGCAGAACC ATGGTCGGCG AGGAGCATCG TCAAGCTTTG 
GTCATAACTC TCCGGGACCC GCCGGTTGCG TACAGGAAGA GGGATGTCTC AAAGACATTT 
GGCGAGGATC GGCTGACTTG GAGCGAGAAT GACCTCTGGG AGAGAGTGGT CGACATTAGC 
CCTGGACTCG ATGTCAGCAA AAACCCAGTT TCGCTCGCCG AGAACCACCA ATATATTGAC 
CTGGGACGGT GGCTTACCTA TTGGATTGAA CTGGATCAAC AATCAACAAG AGTCTGGGAT 
CAAGTTCAAC AATATTTGCT CGATTGGAAT CTCAGGACTA AGTTGACTGT TTTCCCCGAG 
CCGCTTCCCA ACCAAAAACC AAAGGTATGG GATTTTCTGG ATGATAGATA CGGCCATGAC 
ATTCAACAAG TCTCCTCACG GTCCTGGAGC AACGACTTTT CTCTTTTAGC CGCGCCGCCC 
CGTATTTCTC TACCGTTTGA TGTTCGGTAT CAGCTTGAAG TCTGCATATC GCAAGGAATC 
ATCAACGAGC ACAACATTGA CCGGCCGTTC CTTGAGAAGC TGATGGAATT TTGTGATAAT 
GACAACAGCT TCGGCAAAGA TCGGGCTCGC CTCATCCTCG AATACGTCGC CGATGAGTAT 
GCTGGAAAGC GTATCTTCGA CCCGATGGAA CTCTTCAAGG ATCATGCAGC CTTAGCTTAT 
TTCCCAACTA GTTTCATGAT ACCCAACCAT TGCGCTTGGG TTCGTAGGGT CACCATCACC 
CCTACTCGTA TCTACTTCAG TACCCCCTGC GTCGAACCCA CGAACCGCGT CATCCGCCAG 
TGGAAACATG CACAAGACTA CTTTATCCGC ATTCAATTCA CCGATGAAGT TCTTGAAGGA 
CGTATCAAGA GCGGCGAAGC GGAACTTCCC CTATTCTTGC GTGTGTACAG GGTATTGGAG 
AAAGGGGTTG CCATGGGTCC GTGGCATTGG AAGTTCCTTG CATTTGGCAA CTCTCAGATT 
CGAGAAGCCG GAGCCTTCAT GTTCTGTGAA CAGAGCAATT TGACAGGCGA TATGATGCGA 
GCCTGGATGG GCCGCTTCAG TCACATCAAG GTCATTGCCA AGTATGCGGC TAGACTTGGG 
CAGTGTTTCT CAACTACCCG TTTGGTGCCT GGCATCCCGG CACCGAGGAT CGTCACCATC 
CCTGATGTCG AAAAGGACGG ATTTTGCTTC ACGGATGGAG TAGGCAAGAT TTCCCCTTTG 
CTTGCAAAGA TCGTAGCGCA TGACTGGAGC ATTGACCCGC CGCCGTCAGC TTACCAGTTC 
CGCATGGGAG GTTGCAAGGG AGTCTTGGTA ACTTGGCCTG ATGTGAAGGG TATGGAGGTT 
CATATTCGGA AGTCGCAGGA AAAGTTCGTT GCCGAATTCA ATGGCCTTGA GGTTGTCAGA 
TGCTCGCAGT TCTCAACCGC CACTCTCAAC CGGCAAATCA TTGCCGTTCT CTCGTCTCTT 
GGTGTTCCCG ATCAAGTCTT TGTAGACATG ATGGAGCAGC AGCTTTCTGA CTTCAATGCG 
GCGATGGAGG ACAAACAAAA GGCGACGGCG ATTCTGAAAA CGTTCATTGA TGAAAATCAT 
ATGACACCTA TCATTGCGGA GATGCTTGCC TACGGCTTTA TGGGGTCTCA GGAGCCTTTT 
GTTCGCACCC TACTTCAGCT GTGGCGATCA TGGTCGATCA AAACGTTGAA GGAGAAAGCG 
CGACTCAATG TCGAAAAGAG TGCGTTCGTG CTCGGCTGTG TTGATGAGAC TGGCACTCTG 
AAAGGGCACA TGAAGGTCAT TGAAGACTGG AAAGACGTGT CCAGTGAGAA GCTTCCACAG 
ATCTTCCTAC AGATTCCCGA TGATGTCAAT GGCGGGTACA GGGTTATTAC TGGAACCTGC 
GTCGTTGGCA GAAACCCGTC TCTCCATCCT GGTGATATCC GTGTGGTGGA GGCAGTTGAT 
GTCCCTGCTT TGCGGCATCT AAGAGACGTG GTGGTGTTCC CCTTGACTGG GGACAGAGAC 
GTGCCCAGCA TGTGCTCTGG TGGAGACCTC GATGGTGATG ACTTCTTTGT TATTTGGGAT 
CCTTTGCTTA TCCCGAAAGA ACGCTCCCAT CCCCCAATGA TCAGTGAGCC CATTGCGGGC 
AAGGAGTTGG CAACCGAACC GACCGTAAAC AACCTGATTA CCTTCTTTGT GTTGTATATG 
AAGTACAACA ATCTGCCGTT GATCGCTCAT GCTCATCTGG CGACAGCAGA TGCGGAGGTG 
GAAGGAGTGA AGAGCCCGAA ATGCCTCGAG CTCGCTTCAC TTCACTCCAT GGCTGTTGAC 
TACGTGAAGA CTGGTGTTGC CGCCGAGTTT CCGAGACGGC TTGACCCCAA GACCTGGCCT 
CATTTCATGG AGAAAAACAG ACACACTTAC CACTCAGTAA CAGCACTTGG AAAGCTCTAC 
GATATGGTTA AACGGGAAAC ATTCGACATG AAAGAGAACT ACCAGCTGCC GTTTGATAAC 
CGCATCCTCA AGCACACAAA GTGCCGCGCT CTTAGGGATG GGACGCTGGC CAAAGCCCGT 
CGCATCAAAT CTCAGTACGA CACCGCTATG CGCCGCGTGA TGTGCCAGCT CGAAATTGCG 
ACCGAGTTCG AAGTTTGGAC CGCCTTTGTC ATGTCCAAAC CCCGCGTGGG CTCAGACTAC 
AAACTCCAAG ACAACGTCGG TCGCGAGTCT TCTGCCCTCA AGCAACACTT CAAGGATCAG 
TGTAAAAAGG AGGCTGGGGG TGATCTCCTT TCCTTCGTAT CGGCCATGTA TAGAGTCACT 
TACGAAGAAG TTCGCATTGC CCTTTTCGAA GCCAAGCAGC CGCACGTTAG ACCTGACGGC 
AGGCTCGGAA CCAGAAAAAT TACACCAAAG ACCATGCCAC TTGTTAGCTT TCCCTGGCTA 
TTTTGGGACA AGCTCGGTGA GCTTGCCAGG GCAGGCGCCG TATTGCAAAG GAGACTTGAC 
GATGGAAGTG AGGATATGGA CCTTTTGAGC GACGTCCCGC TCGTTTCCCA GAGGCGCAGA 
GGCAAACATA ATGCAAGTGG TAGCAGTGAC TTTATGGATG AGCATGGTGA TCCCCTTTCC 
TATACACGCA CAAGCGATGG CAAGATCATT CACTATGGTC AGATCTTGAA CCTTTTCAGT 
CACGACGATG AAGATGGAGA TGAGAGGAAT GACGCCCGGG ACAGAAACAG TTCCAGCGAA 
GATTCTACTC ATGTTTCTAA TTCGTCCAAA TCCAGCTCCA ACCTTAGCCC GGTTGCTGAG 
GAGGACCTTC TTACTTTTGA GTCGCCTCCA GCTAGTCCAG GTGTCACTCC TGCGTCTCCG 
CAAGTTGATC TCCTAGGCCC GACACTTACG CTCACTAAGG CCGAGCTCGC CATCGGTCTC 
GCTGGTCCGG TTGCCACTAC CTATACTCCT CCGCCGACGG ATGAGGAGAC AGTAATTTGC 
CATGGTCACT CCAACCGCGC CAGTCACCTC AGTGCTTCGT CTTCGTCACT TGATCCTCAA 
CCTCTGATTG AGGAGACTTC CGGGGAGAAC GAGGATCTCC TGCTAGATAT TTATTCTGCG 
TCTCCCCCTA GGGCGGATGG CGCTAGATTA GCCTCGGCCG TTGGAAGTGA TGTTCCTGTC 
AAGGGCGGGC CTCCTCCCGT TTGGATGGAG CAGGTGATCA GAATGGGCCA CAGGATTCCA 
ACCCCTCCTC TTGACTCAAT TGCGATCCCG AATGTGATTG GGGTTCCGCA ATTCGAGAGT 
CCGCTGACTG GGACTGTTGT GGCTTCTGCT ATTCAGGATC CGTTTGTTTC CCCTTCTCCT 
GCGGTTGCCA CTCCTGCTAC CAGTGGTAGT GGTGGTGGTT GGGGTGGTGG TGGTGGTGGT 
GGTGGTGGTG GATATATCGG TATTGGCGGT CCCATCAACA AGGAGCTCGT CATGGGATTG 
GACGGAGGTA TCAAAGAGGA AGATCGCGAG GAAACCGACG AGGAGGCTGA GGAAGTCGAG 
TTGGAGATTG ACGAGGACCC AATTGCAGTG CGTTATGCAC AGATGGCGGC GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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sad-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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