NCU07688 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07688
Entrez Gene ID	3878478
Full Name	rho GTPase activator Rga
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395955.1	XP_011394257.1	rho GTPase activator Rga, variant
XM_011395954.1	XP_011394256.1	rho GTPase activator Rga

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04186	XP_003719637.1
Neurospora crassa	NCU07688	XP_962330.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07688 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07688 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12073	XM_011395954.1 Latest version!	Neurospora crassa OR74A rho GTPase activator Rga (NCU07688), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ONf12074	XM_011395955.1 Latest version!	Neurospora crassa OR74A rho GTPase activator Rga, variant (NCU07688), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07688 ( XM_011395954.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394256.1 Neurospora crassa OR74A rho GTPase activator Rga (NCU07688), mRNA.

Price & Availability↑

CloneID	ONf12073
Clone ID Related Accession (Same CDS sequence)	XM_011395954.1
Accession Version	XM_011395954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	rho GTPase activator Rga
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGGATGACT ATCTTGACGC CCCCATGGAT GCGGACGATG TGTTCCCATG CAAAGGATGC 
GGTGAAATTC TGGAAGAAGG CAAAGCATTC GAACTTGCTG GAAACCGATG GCATCTCGAT 
TGCTTCCGTT GTAACACATG CAACACGCTC CTTGATTCGG ATGCGAATCT CCTTCTGCTT 
GGAGACGGGT CGCTGATTTG CAACAACTGC ACATACAGCT GTAGTGCTTG CGGTAATAAG 
ATTGAGGACT TGGCCATCCT GACCGGAGAC CAGGCCTTTT GCGCAACATG CTTCCGCTGT 
AGGAATTGCA AGAGAAAGAT CGAAAACCTG CGCTACGCCA GGACGGCTCA TGGGATATTT 
TGCATGAATT GCCACGAGAG TCTAATGGCA CGAAGACGCA AGAAAGCAAA AGCAGCGGCT 
CAAGCAAAAG CTCGAGACAC CGACAAATCC CTTCCTGCGC TACCGCCAAA CGCTGTACCC 
CCTTCCAGCA CAGGTTTCGC AGCCGAAAAG CTTGCGGCGG ACTCTGATAC CCCTACCGAA 
CTATCACCAC GACCGCGAAA TGCTTATGGA CGGCAGGACT CATCGTCGCG GAGCAGTTCG 
AGACCTGACC GCTCCCCCGA AAGATTGGCG GAAACGAACA AGGAAACGGT GCTGGCCCCG 
CCAGCGCCAA ACTATAGACA CAACCGCAAT TCAACCATGT TTTCTGCTGA TCTCAGCGGG 
GGGGATGCTG AGGACTTCTT CATTCCCGTC GCGCTTGACC AGAGTCCCGC GCCGTCCATC 
ACGCCTCAGT CCACGGCAGA CACAATTTCA GACACGAGCC GTAAAAACAA AGATTATTTT 
GGCTCGGCCA GGTCAAACGC TGTCCAGCCA GACAAGAAGG AATCGACGGC GCCAACACCC 
CACATTGCAT TGATGGCGCA GATGGCCCAA GAGAGGCAGC CGTCGTCTGA CTATGAAAGT 
CCTCAACAGG AGCGGCTCCC ACCCCGGAAG CTCTCTCGAG CGGCCGATGT GATTGGTAAC 
ACAAAACCGG CCCATATCGC TAGCATCTTG GCCGAAGTCT ACAATCAGCA GCCCAACAAC 
GAGGACTTCA AGCTGCAGAA TGTACCCAAA AGCAAGAGGC TTGTGGGTAA CCGGAGCGAA 
TCTCAAGCAA GCAACAGCAT CGAGAATGGA ACTACGCCAC GGAACAAGGA GAGTCCTGAG 
CCCCCTTTAA CTATGAGTCC AGACAGGATC ATGACCCCTA GGTCCTCTCA GGATTCTCGT 
CCTAGGGATC CCATGAGCGA TGAGATACGG CCGTCCAGCG ACTCTGTAAC CTCGCGCTCA 
GAAGTCTCGA ACGCTGGGCG ATCCATTCCA CGGAAAGAGG TCCCGCCTCC TGTATCCAGA 
AACTTAGCCG ATGGCAAACC TCTGCCGGTT CCTGGGTCGA ACAGTTCCGA GGGTCCTGCT 
CTCCCGATCC GTCCCCCGCC GCCTGAGGCG AAGCTTAGTA ACACGTATAT GCAACCTCGC 
GCACCGCCTT TGCCTCCCAC TCAGGCCCAG ACCCCCAAAA ACCAGTCGAC AACAAGTGGT 
GAAGTCCAGG AGCTGAAACC ATCCCCCAAG CTACCTAGGT GGAGCTCTGG GGTCGAGTTC 
AGCATGGATG AGGATATGGC CAGGATATTG GGAACAGATG AGGGCTCATC GTCAATCCTC 
CGCCGGGTCT CAAACGCAGT ACGACATGGA CGGAACAGCA GTGGTGAAAC AGGCGCTACT 
CCTGGCAGGA TTTCTCACAG TAGGAGCGTG AGCGAAACCA CTCGAGGAAC TACTTCGCCC 
CGTTGGCCGA GAACTCCGAT TGTTGAGGAC TACTCCGCCG GTAATATCAA CGATATAACG 
AGTCCGTTGT CTTTGGCTAG TACGACACAG GATGATCCAG CCTATCTGAA GCGGCAACTC 
AGAAGCTCCG AGCAGCGGGT GGCAGAGCTG GAGCGACAGT TCAACACGGA AAAGGATCTT 
AAGAGTCTTA ACAAGAAGTT GATCGAGAAG AGGAAGACAG TTTCGGTGCT TGATACTCAG 
ACTGAGATCA TGATCAGGCA GCTTGAGGTC TTGGCCGGGT ATGTTGAGCG TGCAAAGAAG 
ACTAACGAGT CGTTTGACGC TCGCGAGTTG GAGGAGTCCG CAATCAAGGA GTTTGTCCAA 
AAGCTAGAAC GTCTCAAGCA CAACATGACC TCTGCGATCG AGCAACTCAT CGCGGAGAGA 
GACGATTTGG TGGAGGAGAA AAACCAAGCC ATTGCCGACC GCGATCGCGC TCTGCTCGAG 
TTTGAGCAGC TGTCTTCCAA GAACGCTCAG TTGGCCGATA TGAACAATGA TCTCACTCAC 
CAGATTCAGG AGAGATTCAA GCAGCAGATC AATAACGGCG AGCTCAAGTC ACCCAATGGT 
TTGGGTATTT ACGGCCACAC CAAGGGTGCT TCACTCAACA TGGACGCCGC GAGCTTACAG 
ACAGCTACGA CTATGGTTGG TCCTGATATG GAAGATCAGC TTGTGGAAGG CCCTACCGTG 
GTGAACATCC GCAAGGGCCA GGTCAAGAAG TTCAACTGGA AGAAGGGCTC CAGCAAGGTG 
GCGCACAATA TCTCGAAGGG TATCCGTGGT GCAGCAGGCG CATTCCAAGG ACAGGAAGGA 
ACCCAGAGGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGGGCTCCTT GAACGGAGAC 
AACATTGGTC TTCCTTATGC CATGACAGTT GCCCAGGTTG AGGCTCCGGT CACTGTCGTT 
CCCCCACCAG CCAACACCAT CAAACCTGTG ATGGTTGATA ACGCGCGGCA AGGTGGAGGA 
GGTGGTGGTG GAGGGTTTGG TTTCTTCGGT GGCAAAAAGC CGAACGCGCA GCAAAAGTCG 
ATGTCGGTGA CCAATATCTC TACCCCTGCG GTTGCAGAAG CCCCCACTAC TCTCTTCGGT 
TCCGACCTGA TTGAGCGGAC TGAACATGAA CGCCGTCAGA TACCAAGCGT GGTCACGCGC 
TGTATCGAGG AGGTTGAGCT TCGTGGTATG GATGTCGAGG GAATCTACAG GAAAACCGGA 
GGCAACTCTC AAATCAAGAT GATTCAAGAG GGCTTTGAGA AGCAGCAAGA TTTCGACATT 
TCCGACCCGG AGATCGATAT TACTGCTGTG ACGAGTGTCC TTAAACAGTA CTTCCGCAAG 
CTCCCCAACC CCTTGCTGAC GTTCGAGGTG TATGAAAGGG TCTTGGAGTC CAATAATATT 
CAAGACGTAG AGGAGAAATG CGCTCACCTG AAGAAAACGG TCAACATGCT TCCACCAAAG 
CACCGCGACT GTCTCGAGTT CCTCATGTTT CATCTTGCTC GAGTAGCGAG CAGAGAACGC 
GAGAATTTGA TGTCACCCAA AAACTTGGCT GTGGTGTTTG CGCCCACCAT CATGAGAGAT 
CACAGTCTCG AGAAAGAAAT GACAGACATG CACGCCAAGA ACAATGCCGT GCAGTTCCTG 
ATCGAGAACA GTGCGCTCAT CTTTGAGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394256.1
CDS	224..3736
Translation	MDDYLDAPMDADDVFPCKGCGEILEEGKAFELAGNRWHLDCFRCNTCNTLLDSDANLLLLGDGSLICNNCTYSCSACGNKIEDLAILTGDQAFCATCFRCRNCKRKIENLRYARTAHGIFCMNCHESLMARRRKKAKAAAQAKARDTDKSLPALPPNAVPPSSTGFAAEKLAADSDTPTELSPRPRNAYGRQDSSSRSSSRPDRSPERLAETNKETVLAPPAPNYRHNRNSTMFSADLSGGDAEDFFIPVALDQSPAPSITPQSTADTISDTSRKNKDYFGSARSNAVQPDKKESTAPTPHIALMAQMAQERQPSSDYESPQQERLPPRKLSRAADVIGNTKPAHIASILAEVYNQQPNNEDFKLQNVPKSKRLVGNRSESQASNSIENGTTPRNKESPEPPLTMSPDRIMTPRSSQDSRPRDPMSDEIRPSSDSVTSRSEVSNAGRSIPRKEVPPPVSRNLADGKPLPVPGSNSSEGPALPIRPPPPEAKLSNTYMQPRAPPLPPTQAQTPKNQSTTSGEVQELKPSPKLPRWSSGVEFSMDEDMARILGTDEGSSSILRRVSNAVRHGRNSSGETGATPGRISHSRSVSETTRGTTSPRWPRTPIVEDYSAGNINDITSPLSLASTTQDDPAYLKRQLRSSEQRVAELERQFNTEKDLKSLNKKLIEKRKTVSVLDTQTEIMIRQLEVLAGYVERAKKTNESFDARELEESAIKEFVQKLERLKHNMTSAIEQLIAERDDLVEEKNQAIADRDRALLEFEQLSSKNAQLADMNNDLTHQIQERFKQQINNGELKSPNGLGIYGHTKGASLNMDAASLQTATTMVGPDMEDQLVEGPTVVNIRKGQVKKFNWKKGSSKVAHNISKGIRGAAGAFQGQEGTQRQQQQQQQQQQQGSLNGDNIGLPYAMTVAQVEAPVTVVPPPANTIKPVMVDNARQGGGGGGGGFGFFGGKKPNAQQKSMSVTNISTPAVAEAPTTLFGSDLIERTEHERRQIPSVVTRCIEEVELRGMDVEGIYRKTGGNSQIKMIQEGFEKQQDFDISDPEIDITAVTSVLKQYFRKLPNPLLTFEVYERVLESNNIQDVEEKCAHLKKTVNMLPPKHRDCLEFLMFHLARVASRERENLMSPKNLAVVFAPTIMRDHSLEKEMTDMHAKNNAVQFLIENSALIFEE

Target ORF information:

RefSeq Version	XM_011395954.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A rho GTPase activator Rga (NCU07688), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395954.1

ORF Insert Sequence:

ATGGATGACT ATCTTGACGC CCCCATGGAT GCGGACGATG TGTTCCCATG CAAAGGATGC 
GGTGAAATTC TGGAAGAAGG CAAAGCATTC GAACTTGCTG GAAACCGATG GCATCTCGAT 
TGCTTCCGTT GTAACACATG CAACACGCTC CTTGATTCGG ATGCGAATCT CCTTCTGCTT 
GGAGACGGGT CGCTGATTTG CAACAACTGC ACATACAGCT GTAGTGCTTG CGGTAATAAG 
ATTGAGGACT TGGCCATCCT GACCGGAGAC CAGGCCTTTT GCGCAACATG CTTCCGCTGT 
AGGAATTGCA AGAGAAAGAT CGAAAACCTG CGCTACGCCA GGACGGCTCA TGGGATATTT 
TGCATGAATT GCCACGAGAG TCTAATGGCA CGAAGACGCA AGAAAGCAAA AGCAGCGGCT 
CAAGCAAAAG CTCGAGACAC CGACAAATCC CTTCCTGCGC TACCGCCAAA CGCTGTACCC 
CCTTCCAGCA CAGGTTTCGC AGCCGAAAAG CTTGCGGCGG ACTCTGATAC CCCTACCGAA 
CTATCACCAC GACCGCGAAA TGCTTATGGA CGGCAGGACT CATCGTCGCG GAGCAGTTCG 
AGACCTGACC GCTCCCCCGA AAGATTGGCG GAAACGAACA AGGAAACGGT GCTGGCCCCG 
CCAGCGCCAA ACTATAGACA CAACCGCAAT TCAACCATGT TTTCTGCTGA TCTCAGCGGG 
GGGGATGCTG AGGACTTCTT CATTCCCGTC GCGCTTGACC AGAGTCCCGC GCCGTCCATC 
ACGCCTCAGT CCACGGCAGA CACAATTTCA GACACGAGCC GTAAAAACAA AGATTATTTT 
GGCTCGGCCA GGTCAAACGC TGTCCAGCCA GACAAGAAGG AATCGACGGC GCCAACACCC 
CACATTGCAT TGATGGCGCA GATGGCCCAA GAGAGGCAGC CGTCGTCTGA CTATGAAAGT 
CCTCAACAGG AGCGGCTCCC ACCCCGGAAG CTCTCTCGAG CGGCCGATGT GATTGGTAAC 
ACAAAACCGG CCCATATCGC TAGCATCTTG GCCGAAGTCT ACAATCAGCA GCCCAACAAC 
GAGGACTTCA AGCTGCAGAA TGTACCCAAA AGCAAGAGGC TTGTGGGTAA CCGGAGCGAA 
TCTCAAGCAA GCAACAGCAT CGAGAATGGA ACTACGCCAC GGAACAAGGA GAGTCCTGAG 
CCCCCTTTAA CTATGAGTCC AGACAGGATC ATGACCCCTA GGTCCTCTCA GGATTCTCGT 
CCTAGGGATC CCATGAGCGA TGAGATACGG CCGTCCAGCG ACTCTGTAAC CTCGCGCTCA 
GAAGTCTCGA ACGCTGGGCG ATCCATTCCA CGGAAAGAGG TCCCGCCTCC TGTATCCAGA 
AACTTAGCCG ATGGCAAACC TCTGCCGGTT CCTGGGTCGA ACAGTTCCGA GGGTCCTGCT 
CTCCCGATCC GTCCCCCGCC GCCTGAGGCG AAGCTTAGTA ACACGTATAT GCAACCTCGC 
GCACCGCCTT TGCCTCCCAC TCAGGCCCAG ACCCCCAAAA ACCAGTCGAC AACAAGTGGT 
GAAGTCCAGG AGCTGAAACC ATCCCCCAAG CTACCTAGGT GGAGCTCTGG GGTCGAGTTC 
AGCATGGATG AGGATATGGC CAGGATATTG GGAACAGATG AGGGCTCATC GTCAATCCTC 
CGCCGGGTCT CAAACGCAGT ACGACATGGA CGGAACAGCA GTGGTGAAAC AGGCGCTACT 
CCTGGCAGGA TTTCTCACAG TAGGAGCGTG AGCGAAACCA CTCGAGGAAC TACTTCGCCC 
CGTTGGCCGA GAACTCCGAT TGTTGAGGAC TACTCCGCCG GTAATATCAA CGATATAACG 
AGTCCGTTGT CTTTGGCTAG TACGACACAG GATGATCCAG CCTATCTGAA GCGGCAACTC 
AGAAGCTCCG AGCAGCGGGT GGCAGAGCTG GAGCGACAGT TCAACACGGA AAAGGATCTT 
AAGAGTCTTA ACAAGAAGTT GATCGAGAAG AGGAAGACAG TTTCGGTGCT TGATACTCAG 
ACTGAGATCA TGATCAGGCA GCTTGAGGTC TTGGCCGGGT ATGTTGAGCG TGCAAAGAAG 
ACTAACGAGT CGTTTGACGC TCGCGAGTTG GAGGAGTCCG CAATCAAGGA GTTTGTCCAA 
AAGCTAGAAC GTCTCAAGCA CAACATGACC TCTGCGATCG AGCAACTCAT CGCGGAGAGA 
GACGATTTGG TGGAGGAGAA AAACCAAGCC ATTGCCGACC GCGATCGCGC TCTGCTCGAG 
TTTGAGCAGC TGTCTTCCAA GAACGCTCAG TTGGCCGATA TGAACAATGA TCTCACTCAC 
CAGATTCAGG AGAGATTCAA GCAGCAGATC AATAACGGCG AGCTCAAGTC ACCCAATGGT 
TTGGGTATTT ACGGCCACAC CAAGGGTGCT TCACTCAACA TGGACGCCGC GAGCTTACAG 
ACAGCTACGA CTATGGTTGG TCCTGATATG GAAGATCAGC TTGTGGAAGG CCCTACCGTG 
GTGAACATCC GCAAGGGCCA GGTCAAGAAG TTCAACTGGA AGAAGGGCTC CAGCAAGGTG 
GCGCACAATA TCTCGAAGGG TATCCGTGGT GCAGCAGGCG CATTCCAAGG ACAGGAAGGA 
ACCCAGAGGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGGGCTCCTT GAACGGAGAC 
AACATTGGTC TTCCTTATGC CATGACAGTT GCCCAGGTTG AGGCTCCGGT CACTGTCGTT 
CCCCCACCAG CCAACACCAT CAAACCTGTG ATGGTTGATA ACGCGCGGCA AGGTGGAGGA 
GGTGGTGGTG GAGGGTTTGG TTTCTTCGGT GGCAAAAAGC CGAACGCGCA GCAAAAGTCG 
ATGTCGGTGA CCAATATCTC TACCCCTGCG GTTGCAGAAG CCCCCACTAC TCTCTTCGGT 
TCCGACCTGA TTGAGCGGAC TGAACATGAA CGCCGTCAGA TACCAAGCGT GGTCACGCGC 
TGTATCGAGG AGGTTGAGCT TCGTGGTATG GATGTCGAGG GAATCTACAG GAAAACCGGA 
GGCAACTCTC AAATCAAGAT GATTCAAGAG GGCTTTGAGA AGCAGCAAGA TTTCGACATT 
TCCGACCCGG AGATCGATAT TACTGCTGTG ACGAGTGTCC TTAAACAGTA CTTCCGCAAG 
CTCCCCAACC CCTTGCTGAC GTTCGAGGTG TATGAAAGGG TCTTGGAGTC CAATAATATT 
CAAGACGTAG AGGAGAAATG CGCTCACCTG AAGAAAACGG TCAACATGCT TCCACCAAAG 
CACCGCGACT GTCTCGAGTT CCTCATGTTT CATCTTGCTC GAGTAGCGAG CAGAGAACGC 
GAGAATTTGA TGTCACCCAA AAACTTGGCT GTGGTGTTTG CGCCCACCAT CATGAGAGAT 
CACAGTCTCG AGAAAGAAAT GACAGACATG CACGCCAAGA ACAATGCCGT GCAGTTCCTG 
ATCGAGAACA GTGCGCTCAT CTTTGAGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU07688 ( XM_011395955.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394257.1 Neurospora crassa OR74A rho GTPase activator Rga, variant (NCU07688), mRNA.

Price & Availability↑

CloneID	ONf12074
Clone ID Related Accession (Same CDS sequence)	XM_011395955.1
Accession Version	XM_011395955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	rho GTPase activator Rga, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGGATGACT ATCTTGACGC CCCCATGGAT GCGGACGATG TGTTCCCATG CAAAGGATGC 
GGTGAAATTC TGGAAGAAGG CAAAGCATTC GAACTTGCTG GAAACCGATG GCATCTCGAT 
TGCTTCCGTT GTAACACATG CAACACGCTC CTTGATTCGG ATGCGAATCT CCTTCTGCTT 
GGAGACGGGT CGCTGATTTG CAACAACTGC ACATACAGCT GTAGTGCTTG CGGTAATAAG 
ATTGAGGACT TGGCCATCCT GACCGGAGAC CAGGCCTTTT GCGCAACATG CTTCCGCTGT 
AGGAATTGCA AGAGAAAGAT CGAAAACCTG CGCTACGCCA GGACGGCTCA TGGGATATTT 
TGCATGAATT GCCACGAGAG TCTAATGGCA CGAAGACGCA AGAAAGCAAA AGCAGCGGCT 
CAAGCAAAAG CTCGAGACAC CGACAAATCC CTTCCTGCGC TACCGCCAAA CGCTGTACCC 
CCTTCCAGCA CAGGTTTCGC AGCCGAAAAG CTTGCGGCGG ACTCTGATAC CCCTACCGAA 
CTATCACCAC GACCGCGAAA TGCTTATGGA CGGCAGGACT CATCGTCGCG GAGCAGTTCG 
AGACCTGACC GCTCCCCCGA AAGATTGGCG GAAACGAACA AGGAAACGGT GCTGGCCCCG 
CCAGCGCCAA ACTATAGACA CAACCGCAAT TCAACCATGT TTTCTGCTGA TCTCAGCGGG 
GGGGATGCTG AGGACTTCTT CATTCCCGTC GCGCTTGACC AGAGTCCCGC GCCGTCCATC 
ACGCCTCAGT CCACGGCAGA CACAATTTCA GACACGAGCC GTAAAAACAA AGATTATTTT 
GGCTCGGCCA GGTCAAACGC TGTCCAGCCA GACAAGAAGG AATCGACGGC GCCAACACCC 
CACATTGCAT TGATGGCGCA GATGGCCCAA GAGAGGCAGC CGTCGTCTGA CTATGAAAGT 
CCTCAACAGG AGCGGCTCCC ACCCCGGAAG CTCTCTCGAG CGGCCGATGT GATTGGTAAC 
ACAAAACCGG CCCATATCGC TAGCATCTTG GCCGAAGTCT ACAATCAGCA GCCCAACAAC 
GAGGACTTCA AGCTGCAGAA TGTACCCAAA AGCAAGAGGC TTGTGGGTAA CCGGAGCGAA 
TCTCAAGCAA GCAACAGCAT CGAGAATGGA ACTACGCCAC GGAACAAGGA GAGTCCTGAG 
CCCCCTTTAA CTATGAGTCC AGACAGGATC ATGACCCCTA GGTCCTCTCA GGATTCTCGT 
CCTAGGGATC CCATGAGCGA TGAGATACGG CCGTCCAGCG ACTCTGTAAC CTCGCGCTCA 
GAAGTCTCGA ACGCTGGGCG ATCCATTCCA CGGAAAGAGG TCCCGCCTCC TGTATCCAGA 
AACTCCGATG GCAAACCTCT GCCGGTTCCT GGGTCGAACA GTTCCGAGGG TCCTGCTCTC 
CCGATCCGTC CCCCGCCGCC TGAGGCGAAG CTTAGTAACA CGTATATGCA ACCTCGCGCA 
CCGCCTTTGC CTCCCACTCA GGCCCAGACC CCCAAAAACC AGTCGACAAC AAGTGGTGAA 
GTCCAGGAGC TGAAACCATC CCCCAAGCTA CCTAGGTGGA GCTCTGGGGT CGAGTTCAGC 
ATGGATGAGG ATATGGCCAG GATATTGGGA ACAGATGAGG GCTCATCGTC AATCCTCCGC 
CGGGTCTCAA ACGCAGTACG ACATGGACGG AACAGCAGTG GTGAAACAGG CGCTACTCCT 
GGCAGGATTT CTCACAGTAG GAGCGTGAGC GAAACCACTC GAGGAACTAC TTCGCCCCGT 
TGGCCGAGAA CTCCGATTGT TGAGGACTAC TCCGCCGGTA ATATCAACGA TATAACGAGT 
CCGTTGTCTT TGGCTAGTAC GACACAGGAT GATCCAGCCT ATCTGAAGCG GCAACTCAGA 
AGCTCCGAGC AGCGGGTGGC AGAGCTGGAG CGACAGTTCA ACACGGAAAA GGATCTTAAG 
AGTCTTAACA AGAAGTTGAT CGAGAAGAGG AAGACAGTTT CGGTGCTTGA TACTCAGACT 
GAGATCATGA TCAGGCAGCT TGAGGTCTTG GCCGGGTATG TTGAGCGTGC AAAGAAGACT 
AACGAGTCGT TTGACGCTCG CGAGTTGGAG GAGTCCGCAA TCAAGGAGTT TGTCCAAAAG 
CTAGAACGTC TCAAGCACAA CATGACCTCT GCGATCGAGC AACTCATCGC GGAGAGAGAC 
GATTTGGTGG AGGAGAAAAA CCAAGCCATT GCCGACCGCG ATCGCGCTCT GCTCGAGTTT 
GAGCAGCTGT CTTCCAAGAA CGCTCAGTTG GCCGATATGA ACAATGATCT CACTCACCAG 
ATTCAGGAGA GATTCAAGCA GCAGATCAAT AACGGCGAGC TCAAGTCACC CAATGGTTTG 
GGTATTTACG GCCACACCAA GGGTGCTTCA CTCAACATGG ACGCCGCGAG CTTACAGACA 
GCTACGACTA TGGTTGGTCC TGATATGGAA GATCAGCTTG TGGAAGGCCC TACCGTGGTG 
AACATCCGCA AGGGCCAGGT CAAGAAGTTC AACTGGAAGA AGGGCTCCAG CAAGGTGGCG 
CACAATATCT CGAAGGGTAT CCGTGGTGCA GCAGGCGCAT TCCAAGGACA GGAAGGAACC 
CAGAGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGG GCTCCTTGAA CGGAGACAAC 
ATTGGTCTTC CTTATGCCAT GACAGTTGCC CAGGTTGAGG CTCCGGTCAC TGTCGTTCCC 
CCACCAGCCA ACACCATCAA ACCTGTGATG GTTGATAACG CGCGGCAAGG TGGAGGAGGT 
GGTGGTGGAG GGTTTGGTTT CTTCGGTGGC AAAAAGCCGA ACGCGCAGCA AAAGTCGATG 
TCGGTGACCA ATATCTCTAC CCCTGCGGTT GCAGAAGCCC CCACTACTCT CTTCGGTTCC 
GACCTGATTG AGCGGACTGA ACATGAACGC CGTCAGATAC CAAGCGTGGT CACGCGCTGT 
ATCGAGGAGG TTGAGCTTCG TGGTATGGAT GTCGAGGGAA TCTACAGGAA AACCGGAGGC 
AACTCTCAAA TCAAGATGAT TCAAGAGGGC TTTGAGAAGC AGCAAGATTT CGACATTTCC 
GACCCGGAGA TCGATATTAC TGCTGTGACG AGTGTCCTTA AACAGTACTT CCGCAAGCTC 
CCCAACCCCT TGCTGACGTT CGAGGTGTAT GAAAGGGTCT TGGAGTCCAA TAATATTCAA 
GACGTAGAGG AGAAATGCGC TCACCTGAAG AAAACGGTCA ACATGCTTCC ACCAAAGCAC 
CGCGACTGTC TCGAGTTCCT CATGTTTCAT CTTGCTCGAG TAGCGAGCAG AGAACGCGAG 
AATTTGATGT CACCCAAAAA CTTGGCTGTG GTGTTTGCGC CCACCATCAT GAGAGATCAC 
AGTCTCGAGA AAGAAATGAC AGACATGCAC GCCAAGAACA ATGCCGTGCA GTTCCTGATC 
GAGAACAGTG CGCTCATCTT TGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394257.1
CDS	224..3733
Translation	MDDYLDAPMDADDVFPCKGCGEILEEGKAFELAGNRWHLDCFRCNTCNTLLDSDANLLLLGDGSLICNNCTYSCSACGNKIEDLAILTGDQAFCATCFRCRNCKRKIENLRYARTAHGIFCMNCHESLMARRRKKAKAAAQAKARDTDKSLPALPPNAVPPSSTGFAAEKLAADSDTPTELSPRPRNAYGRQDSSSRSSSRPDRSPERLAETNKETVLAPPAPNYRHNRNSTMFSADLSGGDAEDFFIPVALDQSPAPSITPQSTADTISDTSRKNKDYFGSARSNAVQPDKKESTAPTPHIALMAQMAQERQPSSDYESPQQERLPPRKLSRAADVIGNTKPAHIASILAEVYNQQPNNEDFKLQNVPKSKRLVGNRSESQASNSIENGTTPRNKESPEPPLTMSPDRIMTPRSSQDSRPRDPMSDEIRPSSDSVTSRSEVSNAGRSIPRKEVPPPVSRNSDGKPLPVPGSNSSEGPALPIRPPPPEAKLSNTYMQPRAPPLPPTQAQTPKNQSTTSGEVQELKPSPKLPRWSSGVEFSMDEDMARILGTDEGSSSILRRVSNAVRHGRNSSGETGATPGRISHSRSVSETTRGTTSPRWPRTPIVEDYSAGNINDITSPLSLASTTQDDPAYLKRQLRSSEQRVAELERQFNTEKDLKSLNKKLIEKRKTVSVLDTQTEIMIRQLEVLAGYVERAKKTNESFDARELEESAIKEFVQKLERLKHNMTSAIEQLIAERDDLVEEKNQAIADRDRALLEFEQLSSKNAQLADMNNDLTHQIQERFKQQINNGELKSPNGLGIYGHTKGASLNMDAASLQTATTMVGPDMEDQLVEGPTVVNIRKGQVKKFNWKKGSSKVAHNISKGIRGAAGAFQGQEGTQRQQQQQQQQQQQGSLNGDNIGLPYAMTVAQVEAPVTVVPPPANTIKPVMVDNARQGGGGGGGGFGFFGGKKPNAQQKSMSVTNISTPAVAEAPTTLFGSDLIERTEHERRQIPSVVTRCIEEVELRGMDVEGIYRKTGGNSQIKMIQEGFEKQQDFDISDPEIDITAVTSVLKQYFRKLPNPLLTFEVYERVLESNNIQDVEEKCAHLKKTVNMLPPKHRDCLEFLMFHLARVASRERENLMSPKNLAVVFAPTIMRDHSLEKEMTDMHAKNNAVQFLIENSALIFEE

Target ORF information:

RefSeq Version	XM_011395955.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A rho GTPase activator Rga, variant (NCU07688), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395955.1

ORF Insert Sequence:

ATGGATGACT ATCTTGACGC CCCCATGGAT GCGGACGATG TGTTCCCATG CAAAGGATGC 
GGTGAAATTC TGGAAGAAGG CAAAGCATTC GAACTTGCTG GAAACCGATG GCATCTCGAT 
TGCTTCCGTT GTAACACATG CAACACGCTC CTTGATTCGG ATGCGAATCT CCTTCTGCTT 
GGAGACGGGT CGCTGATTTG CAACAACTGC ACATACAGCT GTAGTGCTTG CGGTAATAAG 
ATTGAGGACT TGGCCATCCT GACCGGAGAC CAGGCCTTTT GCGCAACATG CTTCCGCTGT 
AGGAATTGCA AGAGAAAGAT CGAAAACCTG CGCTACGCCA GGACGGCTCA TGGGATATTT 
TGCATGAATT GCCACGAGAG TCTAATGGCA CGAAGACGCA AGAAAGCAAA AGCAGCGGCT 
CAAGCAAAAG CTCGAGACAC CGACAAATCC CTTCCTGCGC TACCGCCAAA CGCTGTACCC 
CCTTCCAGCA CAGGTTTCGC AGCCGAAAAG CTTGCGGCGG ACTCTGATAC CCCTACCGAA 
CTATCACCAC GACCGCGAAA TGCTTATGGA CGGCAGGACT CATCGTCGCG GAGCAGTTCG 
AGACCTGACC GCTCCCCCGA AAGATTGGCG GAAACGAACA AGGAAACGGT GCTGGCCCCG 
CCAGCGCCAA ACTATAGACA CAACCGCAAT TCAACCATGT TTTCTGCTGA TCTCAGCGGG 
GGGGATGCTG AGGACTTCTT CATTCCCGTC GCGCTTGACC AGAGTCCCGC GCCGTCCATC 
ACGCCTCAGT CCACGGCAGA CACAATTTCA GACACGAGCC GTAAAAACAA AGATTATTTT 
GGCTCGGCCA GGTCAAACGC TGTCCAGCCA GACAAGAAGG AATCGACGGC GCCAACACCC 
CACATTGCAT TGATGGCGCA GATGGCCCAA GAGAGGCAGC CGTCGTCTGA CTATGAAAGT 
CCTCAACAGG AGCGGCTCCC ACCCCGGAAG CTCTCTCGAG CGGCCGATGT GATTGGTAAC 
ACAAAACCGG CCCATATCGC TAGCATCTTG GCCGAAGTCT ACAATCAGCA GCCCAACAAC 
GAGGACTTCA AGCTGCAGAA TGTACCCAAA AGCAAGAGGC TTGTGGGTAA CCGGAGCGAA 
TCTCAAGCAA GCAACAGCAT CGAGAATGGA ACTACGCCAC GGAACAAGGA GAGTCCTGAG 
CCCCCTTTAA CTATGAGTCC AGACAGGATC ATGACCCCTA GGTCCTCTCA GGATTCTCGT 
CCTAGGGATC CCATGAGCGA TGAGATACGG CCGTCCAGCG ACTCTGTAAC CTCGCGCTCA 
GAAGTCTCGA ACGCTGGGCG ATCCATTCCA CGGAAAGAGG TCCCGCCTCC TGTATCCAGA 
AACTCCGATG GCAAACCTCT GCCGGTTCCT GGGTCGAACA GTTCCGAGGG TCCTGCTCTC 
CCGATCCGTC CCCCGCCGCC TGAGGCGAAG CTTAGTAACA CGTATATGCA ACCTCGCGCA 
CCGCCTTTGC CTCCCACTCA GGCCCAGACC CCCAAAAACC AGTCGACAAC AAGTGGTGAA 
GTCCAGGAGC TGAAACCATC CCCCAAGCTA CCTAGGTGGA GCTCTGGGGT CGAGTTCAGC 
ATGGATGAGG ATATGGCCAG GATATTGGGA ACAGATGAGG GCTCATCGTC AATCCTCCGC 
CGGGTCTCAA ACGCAGTACG ACATGGACGG AACAGCAGTG GTGAAACAGG CGCTACTCCT 
GGCAGGATTT CTCACAGTAG GAGCGTGAGC GAAACCACTC GAGGAACTAC TTCGCCCCGT 
TGGCCGAGAA CTCCGATTGT TGAGGACTAC TCCGCCGGTA ATATCAACGA TATAACGAGT 
CCGTTGTCTT TGGCTAGTAC GACACAGGAT GATCCAGCCT ATCTGAAGCG GCAACTCAGA 
AGCTCCGAGC AGCGGGTGGC AGAGCTGGAG CGACAGTTCA ACACGGAAAA GGATCTTAAG 
AGTCTTAACA AGAAGTTGAT CGAGAAGAGG AAGACAGTTT CGGTGCTTGA TACTCAGACT 
GAGATCATGA TCAGGCAGCT TGAGGTCTTG GCCGGGTATG TTGAGCGTGC AAAGAAGACT 
AACGAGTCGT TTGACGCTCG CGAGTTGGAG GAGTCCGCAA TCAAGGAGTT TGTCCAAAAG 
CTAGAACGTC TCAAGCACAA CATGACCTCT GCGATCGAGC AACTCATCGC GGAGAGAGAC 
GATTTGGTGG AGGAGAAAAA CCAAGCCATT GCCGACCGCG ATCGCGCTCT GCTCGAGTTT 
GAGCAGCTGT CTTCCAAGAA CGCTCAGTTG GCCGATATGA ACAATGATCT CACTCACCAG 
ATTCAGGAGA GATTCAAGCA GCAGATCAAT AACGGCGAGC TCAAGTCACC CAATGGTTTG 
GGTATTTACG GCCACACCAA GGGTGCTTCA CTCAACATGG ACGCCGCGAG CTTACAGACA 
GCTACGACTA TGGTTGGTCC TGATATGGAA GATCAGCTTG TGGAAGGCCC TACCGTGGTG 
AACATCCGCA AGGGCCAGGT CAAGAAGTTC AACTGGAAGA AGGGCTCCAG CAAGGTGGCG 
CACAATATCT CGAAGGGTAT CCGTGGTGCA GCAGGCGCAT TCCAAGGACA GGAAGGAACC 
CAGAGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGG GCTCCTTGAA CGGAGACAAC 
ATTGGTCTTC CTTATGCCAT GACAGTTGCC CAGGTTGAGG CTCCGGTCAC TGTCGTTCCC 
CCACCAGCCA ACACCATCAA ACCTGTGATG GTTGATAACG CGCGGCAAGG TGGAGGAGGT 
GGTGGTGGAG GGTTTGGTTT CTTCGGTGGC AAAAAGCCGA ACGCGCAGCA AAAGTCGATG 
TCGGTGACCA ATATCTCTAC CCCTGCGGTT GCAGAAGCCC CCACTACTCT CTTCGGTTCC 
GACCTGATTG AGCGGACTGA ACATGAACGC CGTCAGATAC CAAGCGTGGT CACGCGCTGT 
ATCGAGGAGG TTGAGCTTCG TGGTATGGAT GTCGAGGGAA TCTACAGGAA AACCGGAGGC 
AACTCTCAAA TCAAGATGAT TCAAGAGGGC TTTGAGAAGC AGCAAGATTT CGACATTTCC 
GACCCGGAGA TCGATATTAC TGCTGTGACG AGTGTCCTTA AACAGTACTT CCGCAAGCTC 
CCCAACCCCT TGCTGACGTT CGAGGTGTAT GAAAGGGTCT TGGAGTCCAA TAATATTCAA 
GACGTAGAGG AGAAATGCGC TCACCTGAAG AAAACGGTCA ACATGCTTCC ACCAAAGCAC 
CGCGACTGTC TCGAGTTCCT CATGTTTCAT CTTGCTCGAG TAGCGAGCAG AGAACGCGAG 
AATTTGATGT CACCCAAAAA CTTGGCTGTG GTGTTTGCGC CCACCATCAT GAGAGATCAC 
AGTCTCGAGA AAGAAATGAC AGACATGCAC GCCAAGAACA ATGCCGTGCA GTTCCTGATC 
GAGAACAGTG CGCTCATCTT TGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07688 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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