do cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	do
Entrez Gene ID	3874127
Full Name	doily
Synonyms	fks
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_952887.3	XP_957980.1	1,3-beta-glucan synthase component GLS1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACL181C	NP_983223.2
Magnaporthe oryzae (rice blast fungus)	MGG_00865	XP_003718107.1
Schizosaccharomyces pombe (fission yeast)	bgs2	NP_594032.1
Schizosaccharomyces pombe (fission yeast)	bgs4	NP_588501.1
Neurospora crassa	NCU06871	XP_957980.1
Kluyveromyces lactis	KLLA0B05841g	XP_451796.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following do gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the do cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00018	XM_952887.3 Latest version!	Neurospora crassa OR74A 1,3-beta-glucan synthase component GLS1 (do), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

do ( XM_952887.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957980.1 Neurospora crassa OR74A 1,3-beta-glucan synthase component GLS1 (do), mRNA.

Price & Availability↑

CloneID	ONf00018
Clone ID Related Accession (Same CDS sequence)	XM_952887.3
Accession Version	XM_952887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	1,3-beta-glucan synthase component GLS1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_952887.2.

ATGTCGGGAT ATCCTCAGCA AGGCGGCGGA GGCCATCACG ACGATGGCTA CGGCCATGCT 
CCCAACGCAA ATGGAAATGG TGACGCGTAT TACAACGACG ATCAGCAGTA CTATGATAAC 
CGTAATGGAG GACACGCTGC CGGCGGTCAG CATGGCGAAG GTTACTATGA TGAGTCCGGC 
TACTATAACG CCGACCCAAA CAACCCGTAC CACCAGGATG GTGGTTATTA CGACAACCAT 
GAAGGGTTTC AGGAGGGTTA CGACAACGGC TACTACGACC AGCATGGCTA CGACCAGGCC 
GGCGGCTATC GAGACAACCA TGCCGCTCGT GGCTCTGAGG AGGATTCCGA GACCTTTAGC 
GATTTCACCA TGAGGTCCGA CATGGCCCGT GCCGCCGAGA TGGACTACTA TGGCCGTGGT 
GATGAGCGCT ACGACAGTCA GTATGGCGAC CAGGGCGGTG CCCGGGGTTA TCGCCCTCCT 
TCCTCGCAGA TCTCGTACGG CGGAAACCGC TCGTCTGGCG CGTCGACACC AAACTACGGC 
ATGGACTACG GTAACGTTCT TCCTGCCGGC CAGCGCTCCA AGGAACCGTA TCCCGCCTGG 
ACGTCGGATG CCCAAATCCC CCTCTCGAAG GAGGAGGTGG AGGACATCTT TTTGGATCTA 
TGCGCAAAGT TCGGTTTCCA GCGTGACAGC ATGCGCAACA TGTACGACCA TCTCATGACG 
CTTCTCGACT CCCGTGCTTC TCGTATGACT CCCAACCAGG CCCTTCTCTC GCTCCATGCC 
GACTACATCG GTGGCGACAA CGCAAACTAC CGCAAGTGGT ACTTCGCTGC CCATCTGGAT 
CTGGACGATG CCGTCGGTTT TGCCAACATC AAGGGCAAGA AGGGCAACCT GAAGCGCACC 
AAGAAAAAGG CCAAGGGTGA CGAGCCCCAA AACGAAGCCG AGATCCTTCA AGAGCTGGAG 
GGTGATGACT CGTTGGAGGC GGCCGAGTTC CGTTGGAAGA CTCGCATGAA CAGGATGTCG 
CAGCATGACC GTGTGCGACA GCTTGCCCTC TATCTCCTGA TTTGGGGCGA AGCCAACCAG 
GTCCGCTTCA TGCCCGAGTG TCTGTGCTTC CTCTTCAAGT GCGCCGATGA CTATCTCAAC 
TCGCCCGCGT GCCAAAACAT GGTCGAGCCC GTTGAGGAAT TCACCTTTCT CAACAACGTC 
ATCACCCCGC TGTATCGCTA CTGCCGCGAT CAGGGTTACG AGATCTACGA AGGTGTCTAC 
GTCCGTCGCG AAAGAGATCA CGAGCAGATT ATCGGTTATG ATGACTGCAA CCAGCTCTTC 
TGGTATCCCG AGGGTATCAA CCGTATCGTT CTTGAGGACA AGAGCAAGCT GGTGGACGTC 
CCTCCCGCTG AGCGTTATCT CAAGCTCAAG GATGTCAACT GGAAGAAGTG CTTCTTCAAG 
ACCTACAGGG AGACACGCTC GTGGTTCCAC ATGCTTGTCA ACTTCAACCG TATCTGGATT 
ATTCACTTGA CCATGTTCTG GTTCTACACT GCCTACAACA TGCCTACCAT CATCACTCCC 
ATGTACGAAC AGCAGGTTAA CCAGTCACCT CCCAAGGCTG CCATGTGGTC CTTTGTCGGT 
TTCGGTGGTG GTGTCGCTGC GCTGATCAAC TTTGGAGCAA CTCTGGCAGA ATGGGCCTAT 
GTTCCTCGCA GATGGGCTGG TGCGCAGCAT CTGTCGAAGC GAATGCTGTT CATGGTTTTC 
GTCCTCATCA TCAACTTGGC TCCTGGTGTC TATGTCTTCC TTCCGGGCCT GAAAGGCCAG 
GCTCTGATTG ATCATCAGAA CAGCACCCCT GTCTATATCG TCGGCATCGT TCACTTCTTT 
ATCGCTCTCA TCACCTTCTT GTTCTTTGCC GTGATGCCTC TGGGTGGTCT CTTCGGCAGT 
TACCTCACCA AGAACTCACG CAAGTACGTC GCCAGTCAGA CATTTACTGC CAGCTGGCCT 
CGCCTCAACG GTCATGACAT GGCCATGTCG TTTGGTCTGT GGGTTGTGGT TTTCGGCGCC 
AAGTTCGGAG AGTCGTACGT GTATTTGACA CTGTCGATTC GTGACCCAAT TCGTTACATT 
GGTCTTATGG ATACCAGTTC ATGTCTTGGA GACTCGATTT TGAAGACCTG GCTCTGCCCG 
TACCAGCCGC AAATCACGAT GGGTCTGATG ATCTTCACTG GTATGATCTT CTTCTTCCTC 
GATACGTATC TGTGGTACGT CTTGATCAAC TCCGTCTTCT CGGTCGCCAG AGCTTTCTAC 
CTTGGTTCCT CGATCTGGAC TCCTTGGAGA AACGTCTACG CTCGCTTGCC CAAGCGTATC 
TACTCCAAGG TTCTCGCCAC TACGGACATG GAGATCAAGT ACAAGCCCAA GGTCCTCATC 
TCCCAGATCT GGAATGCCAT CGTCATCTCC ATGTACCGCG AGCACCTTCT GGCCATTGAT 
CACGTTCAGA AGCTCCTCTA CCACCAGGTG CCCTCTGAGC AGGAAGGCAA GCGTACTCTG 
CGTGCCCCCA CTTTCTTCGT TTCCCAGGAA GACCAGTCTT TCAAGACCGA GTTCTTCCCT 
CAGTACAGCG AAGCCGAGCG CAGAATTTCC TTCTTCGCCC AGTCGCTCTC TACTCCCATC 
CCCGAGCCCC TCCCTGTTGA CAACATGCCT ACCTTCACTG TCATGATTCC TCACTACAGC 
GAAAAGATTC TTCTTTCTCT GCGTGAAATC ATTCGTGAAG ATGAGCCCTA CTCGCGTGTC 
ACTCTCCTGG AATACCTCAA GCAGCTCCAT CCCCACGAAT GGGACTGCTT CGTTAAGGAC 
ACCAAGATTC TCGCGGATGA GACCTCGCAG TTCAACGGCG AGACTGAGAA GGAGAAGGAA 
AAGGAAAAGG AGAAGGAGAC CGTCAAGAGC AAGATCGACG ATCTTCCCTT CTACTGCATC 
GGTTTCAAGT CCTCGGCACC CGAGTATACC CTCAGAACCC GTATTTGGGC CTCGCTTCGC 
TTCCAGACCT TGTACCGTAC CGTCTCGGGT TTCATGAACT ACGCTCGTGC CATTAAGCTT 
CTCTACCGTG TCGAGAACCC CGAAGTCGTC CAGATGTTTG GCGGTAACTC CGACAAGCTC 
GAGCGTGAGC TGGAGCGCAT GGCTCGTCGC AAGTTCAAGC TCTGCATTTC CATGCAGCGT 
TTCGCCAAGT TCAAGAAGGA GGAAATGGAG AACGCCGAGT TCTTGTTGCG CGCTTACCCC 
GATCTCCAAA TCGCCTACCT TGACGAAGAG CCTCCTCTTG CCGAAGGTGA GGAGCCTCGT 
CTGTACTCTG CTCTCATCGA CGGTCACTCT GAGATCATGG AGAACGGTTC GCGCCGGCCC 
AAGTTCCGTA TCCAGCTTTC CGGAAACCCC ATTCTCGGTG ACGGAAAGTC CGACAACCAG 
AACCACGCCA TCATCTTCTA CCGCGGCGAG TACATCCAGC TTATCGATGC CAACCAAGAC 
AACTATCTCG AGGAGTGCCT CAAGATCCGC AGCGTGTTGG CCGAGTTTGA GGAGATGAAG 
ACCGACAACC TTTCGCCCTA CACTCCCGGT GTCAAGAACG AGGTCCGCCA CCCTGTTGCC 
ATTCTCGGTG CTCGTGAGTA CATTTTCTCC GAGAACATTG GTATCCTCGG TGATATTGCC 
GCCGGTAAGG AACAGACATT CGGTACGCTC TTCGCCAGAA CCTTGGCCCA GATTGGTGGC 
AAGCTCCATT ACGGTCATCC TGATTTCCTC AACGGTATCT TCATGACTAC CCGTGGTGGT 
GTCTCCAAGG CTCAAAAGGG TTTGCATCTG AACGAGGATA TTTACGCTGG TATGAACGCT 
CTTCTGCGTG GTGGCCGCAT CAAGCACTGC GATTACTACC AGTGCGGTAA GGGTCGTGAT 
TTGGGTTTCG GCTCCATTCT CAACTTCACC ACCAAGATTG GAACTGGTAT GGGTGAGCAG 
CTGCTCTCTC GCGAGTACCA CTACCTTGGA ACTCAGCTCC CGATCGATCG TTTCCTGTCC 
TTCTACTACG CCCATCCCGG TTTCCACTTG AACAACATGT TCATTATGCT CTCGGTCCAG 
CTCTTCATGC TTTGCTGCGT TAACATCGGC GTACTGCGTC ATGAGACGAT CCGCTGCGAG 
TACAACCGCG AGGTCCCCAT TACCGATGCC CTCTTCCCCA CCGGCTGCTC CAACACCGAC 
GCTCTTCTGG ACTGGGTTTA CCGCTGTGTC CTCTCGATTA TCTTCGTCTT GTTCCTTGCC 
TTCGTTCCCC TGATAGTCCA GGAGATGATG GAGAAGGGTG TGATCCGCTC TGCGACTCGT 
TTCATCAAGC AGATCCTTTC GCTCTCGCCC TTCTTCGAAG TGTTCGTCTG TCAGATCTAC 
GCCAACTCTG TCCAGCAAGA TTTGTCCTTC GGTGGTGCCA GATATATCGG TACCGGTCGT 
GGTTTCGCCA CCGCGCGTAT TCCCTTTGGT GTTCTCTACT CCCGTTTCGC CGGTCCTTCG 
ATCTACTTCG GAGCCAGATT GGTCATGATG CTGCTCTTTG CCTGCCTGAC TGTCTGGCAC 
GCTGCCCTGA TCTATTTCTG GATCTCGCTT ATGGCCTTGG TCATCTCGCC CTTCCTCTAC 
AACCCTCACC AGTTCTCTTG GGGCGATTTC TTCATCGACT ACCGCGAGTA TCTCCGCTGG 
CTCTCGCGTG GTAACTCTCG CTCGCACGCC TCTTCTTGGA TCACCTTCTG CCGTCTCTCG 
CGTATTCGAA TCACTGGTTT CAAGCGCAAG ATTATCGGAG ACCCGTCGAG CAAGCTTTCG 
GGCGATGTTG CCAGGGCTGC CATCACCAAC CTGTTCTGGT CCGAGATGCT TACCCCCTTC 
ATCCTTGTCT GCTTGACGAC CATCCCGTAT CTGTTCATCA ACGCGCAGAC TGGTGTCAGG 
GGCGAATTTG GACCCCATGC CAACGACGAT AGCATCAAGC CTACCGCCTC CTTGATCCGT 
CTCTTGGTTG TCACGTTTGC GCCCATGGCT GTCAACGCCG GTGTCCTTGG TGGCTTGTTC 
GGTATGGCCT GCTGCATGGG TCCTCTTTTG AGCATGTGCT GCAAGAAGTT TGGTAGTGTT 
CTGGCCGCCA TTGCCCATGG TATGTCGGTC ATTATCCTTT TGGTTATGTT CGAAGTCATG 
TTCGTCCTGC AGAACTTTGA GTTTACCCCT ACCCTTCTCG GCATGATCGC CATGGTCTCG 
ATTCAGCGCT TCATCATCAA GATGATTGTC TCGTTGGCCT TGACTCGTGA GTTCAAGACT 
GATACCTCCA ACATTGCCTT CTGGACGGGC AAGTGGTACT CTATGGGCTG GCACTCGGTT 
TCGCAACCTG CTCGTGAGTG GCTTGCCAAG ATCACGGAGC TCAGCATGTT TGGCGCCGAC 
TTTATCCTTG GCCACCTGAT TCTGTTCTCC ATGTTCCCCG TCCTTATCAT CCCCAAGGTC 
GATGTGCTCC ACTCGGTCAT TCTCTTCTGG CTCCGTCCAA GCCGTCAAAT TCGTGCCCCC 
ATCTACTCGC TGAAGCAGAC GAAGCTCAGG AGGAGACGTG TGATCCGTTA TGCGATGCTC 
TACTTTGTCC TGCTTGTCAT CTTTGTCGCC CTGATTGTTG GACCGATTGT GGCCGGCAAA 
CAGATTCCTC CTGGCACGCT CGACATGGTC AACAATTTTG GCGGTGGAAG CATGATCCTC 
AAGCAGTACC CCTGGAAGCT GGGACCTTCT GGTGACAATG ACGACACCAG AGGTCGTCTG 
GAAACGGGAA CCAAGGCTAC TAACTACTCT GGTGTCTGGA CCCCGACGAC AACATCGGAC 
TATGTCGCCA AGGCTACTAG CAAGGCTGAA AGGAGGATGG CATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_957980.1
CDS	1184..7051
Translation	MSGYPQQGGGGHHDDGYGHAPNANGNGDAYYNDDQQYYDNRNGGHAAGGQHGEGYYDESGYYNADPNNPYHQDGGYYDNHEGFQEGYDNGYYDQHGYDQAGGYRDNHAARGSEEDSETFSDFTMRSDMARAAEMDYYGRGDERYDSQYGDQGGARGYRPPSSQISYGGNRSSGASTPNYGMDYGNVLPAGQRSKEPYPAWTSDAQIPLSKEEVEDIFLDLCAKFGFQRDSMRNMYDHLMTLLDSRASRMTPNQALLSLHADYIGGDNANYRKWYFAAHLDLDDAVGFANIKGKKGNLKRTKKKAKGDEPQNEAEILQELEGDDSLEAAEFRWKTRMNRMSQHDRVRQLALYLLIWGEANQVRFMPECLCFLFKCADDYLNSPACQNMVEPVEEFTFLNNVITPLYRYCRDQGYEIYEGVYVRRERDHEQIIGYDDCNQLFWYPEGINRIVLEDKSKLVDVPPAERYLKLKDVNWKKCFFKTYRETRSWFHMLVNFNRIWIIHLTMFWFYTAYNMPTIITPMYEQQVNQSPPKAAMWSFVGFGGGVAALINFGATLAEWAYVPRRWAGAQHLSKRMLFMVFVLIINLAPGVYVFLPGLKGQALIDHQNSTPVYIVGIVHFFIALITFLFFAVMPLGGLFGSYLTKNSRKYVASQTFTASWPRLNGHDMAMSFGLWVVVFGAKFGESYVYLTLSIRDPIRYIGLMDTSSCLGDSILKTWLCPYQPQITMGLMIFTGMIFFFLDTYLWYVLINSVFSVARAFYLGSSIWTPWRNVYARLPKRIYSKVLATTDMEIKYKPKVLISQIWNAIVISMYREHLLAIDHVQKLLYHQVPSEQEGKRTLRAPTFFVSQEDQSFKTEFFPQYSEAERRISFFAQSLSTPIPEPLPVDNMPTFTVMIPHYSEKILLSLREIIREDEPYSRVTLLEYLKQLHPHEWDCFVKDTKILADETSQFNGETEKEKEKEKEKETVKSKIDDLPFYCIGFKSSAPEYTLRTRIWASLRFQTLYRTVSGFMNYARAIKLLYRVENPEVVQMFGGNSDKLERELERMARRKFKLCISMQRFAKFKKEEMENAEFLLRAYPDLQIAYLDEEPPLAEGEEPRLYSALIDGHSEIMENGSRRPKFRIQLSGNPILGDGKSDNQNHAIIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEEMKTDNLSPYTPGVKNEVRHPVAILGAREYIFSENIGILGDIAAGKEQTFGTLFARTLAQIGGKLHYGHPDFLNGIFMTTRGGVSKAQKGLHLNEDIYAGMNALLRGGRIKHCDYYQCGKGRDLGFGSILNFTTKIGTGMGEQLLSREYHYLGTQLPIDRFLSFYYAHPGFHLNNMFIMLSVQLFMLCCVNIGVLRHETIRCEYNREVPITDALFPTGCSNTDALLDWVYRCVLSIIFVLFLAFVPLIVQEMMEKGVIRSATRFIKQILSLSPFFEVFVCQIYANSVQQDLSFGGARYIGTGRGFATARIPFGVLYSRFAGPSIYFGARLVMMLLFACLTVWHAALIYFWISLMALVISPFLYNPHQFSWGDFFIDYREYLRWLSRGNSRSHASSWITFCRLSRIRITGFKRKIIGDPSSKLSGDVARAAITNLFWSEMLTPFILVCLTTIPYLFINAQTGVRGEFGPHANDDSIKPTASLIRLLVVTFAPMAVNAGVLGGLFGMACCMGPLLSMCCKKFGSVLAAIAHGMSVIILLVMFEVMFVLQNFEFTPTLLGMIAMVSIQRFIIKMIVSLALTREFKTDTSNIAFWTGKWYSMGWHSVSQPAREWLAKITELSMFGADFILGHLILFSMFPVLIIPKVDVLHSVILFWLRPSRQIRAPIYSLKQTKLRRRRVIRYAMLYFVLLVIFVALIVGPIVAGKQIPPGTLDMVNNFGGGSMILKQYPWKLGPSGDNDDTRGRLETGTKATNYSGVWTPTTTSDYVAKATSKAERRMAF

Target ORF information:

RefSeq Version	XM_952887.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A 1,3-beta-glucan synthase component GLS1 (do), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_952887.3

ORF Insert Sequence:

ATGTCGGGAT ATCCTCAGCA AGGCGGCGGA GGCCATCACG ACGATGGCTA CGGCCATGCT 
CCCAACGCAA ATGGAAATGG TGACGCGTAT TACAACGACG ATCAGCAGTA CTATGATAAC 
CGTAATGGAG GACACGCTGC CGGCGGTCAG CATGGCGAAG GTTACTATGA TGAGTCCGGC 
TACTATAACG CCGACCCAAA CAACCCGTAC CACCAGGATG GTGGTTATTA CGACAACCAT 
GAAGGGTTTC AGGAGGGTTA CGACAACGGC TACTACGACC AGCATGGCTA CGACCAGGCC 
GGCGGCTATC GAGACAACCA TGCCGCTCGT GGCTCTGAGG AGGATTCCGA GACCTTTAGC 
GATTTCACCA TGAGGTCCGA CATGGCCCGT GCCGCCGAGA TGGACTACTA TGGCCGTGGT 
GATGAGCGCT ACGACAGTCA GTATGGCGAC CAGGGCGGTG CCCGGGGTTA TCGCCCTCCT 
TCCTCGCAGA TCTCGTACGG CGGAAACCGC TCGTCTGGCG CGTCGACACC AAACTACGGC 
ATGGACTACG GTAACGTTCT TCCTGCCGGC CAGCGCTCCA AGGAACCGTA TCCCGCCTGG 
ACGTCGGATG CCCAAATCCC CCTCTCGAAG GAGGAGGTGG AGGACATCTT TTTGGATCTA 
TGCGCAAAGT TCGGTTTCCA GCGTGACAGC ATGCGCAACA TGTACGACCA TCTCATGACG 
CTTCTCGACT CCCGTGCTTC TCGTATGACT CCCAACCAGG CCCTTCTCTC GCTCCATGCC 
GACTACATCG GTGGCGACAA CGCAAACTAC CGCAAGTGGT ACTTCGCTGC CCATCTGGAT 
CTGGACGATG CCGTCGGTTT TGCCAACATC AAGGGCAAGA AGGGCAACCT GAAGCGCACC 
AAGAAAAAGG CCAAGGGTGA CGAGCCCCAA AACGAAGCCG AGATCCTTCA AGAGCTGGAG 
GGTGATGACT CGTTGGAGGC GGCCGAGTTC CGTTGGAAGA CTCGCATGAA CAGGATGTCG 
CAGCATGACC GTGTGCGACA GCTTGCCCTC TATCTCCTGA TTTGGGGCGA AGCCAACCAG 
GTCCGCTTCA TGCCCGAGTG TCTGTGCTTC CTCTTCAAGT GCGCCGATGA CTATCTCAAC 
TCGCCCGCGT GCCAAAACAT GGTCGAGCCC GTTGAGGAAT TCACCTTTCT CAACAACGTC 
ATCACCCCGC TGTATCGCTA CTGCCGCGAT CAGGGTTACG AGATCTACGA AGGTGTCTAC 
GTCCGTCGCG AAAGAGATCA CGAGCAGATT ATCGGTTATG ATGACTGCAA CCAGCTCTTC 
TGGTATCCCG AGGGTATCAA CCGTATCGTT CTTGAGGACA AGAGCAAGCT GGTGGACGTC 
CCTCCCGCTG AGCGTTATCT CAAGCTCAAG GATGTCAACT GGAAGAAGTG CTTCTTCAAG 
ACCTACAGGG AGACACGCTC GTGGTTCCAC ATGCTTGTCA ACTTCAACCG TATCTGGATT 
ATTCACTTGA CCATGTTCTG GTTCTACACT GCCTACAACA TGCCTACCAT CATCACTCCC 
ATGTACGAAC AGCAGGTTAA CCAGTCACCT CCCAAGGCTG CCATGTGGTC CTTTGTCGGT 
TTCGGTGGTG GTGTCGCTGC GCTGATCAAC TTTGGAGCAA CTCTGGCAGA ATGGGCCTAT 
GTTCCTCGCA GATGGGCTGG TGCGCAGCAT CTGTCGAAGC GAATGCTGTT CATGGTTTTC 
GTCCTCATCA TCAACTTGGC TCCTGGTGTC TATGTCTTCC TTCCGGGCCT GAAAGGCCAG 
GCTCTGATTG ATCATCAGAA CAGCACCCCT GTCTATATCG TCGGCATCGT TCACTTCTTT 
ATCGCTCTCA TCACCTTCTT GTTCTTTGCC GTGATGCCTC TGGGTGGTCT CTTCGGCAGT 
TACCTCACCA AGAACTCACG CAAGTACGTC GCCAGTCAGA CATTTACTGC CAGCTGGCCT 
CGCCTCAACG GTCATGACAT GGCCATGTCG TTTGGTCTGT GGGTTGTGGT TTTCGGCGCC 
AAGTTCGGAG AGTCGTACGT GTATTTGACA CTGTCGATTC GTGACCCAAT TCGTTACATT 
GGTCTTATGG ATACCAGTTC ATGTCTTGGA GACTCGATTT TGAAGACCTG GCTCTGCCCG 
TACCAGCCGC AAATCACGAT GGGTCTGATG ATCTTCACTG GTATGATCTT CTTCTTCCTC 
GATACGTATC TGTGGTACGT CTTGATCAAC TCCGTCTTCT CGGTCGCCAG AGCTTTCTAC 
CTTGGTTCCT CGATCTGGAC TCCTTGGAGA AACGTCTACG CTCGCTTGCC CAAGCGTATC 
TACTCCAAGG TTCTCGCCAC TACGGACATG GAGATCAAGT ACAAGCCCAA GGTCCTCATC 
TCCCAGATCT GGAATGCCAT CGTCATCTCC ATGTACCGCG AGCACCTTCT GGCCATTGAT 
CACGTTCAGA AGCTCCTCTA CCACCAGGTG CCCTCTGAGC AGGAAGGCAA GCGTACTCTG 
CGTGCCCCCA CTTTCTTCGT TTCCCAGGAA GACCAGTCTT TCAAGACCGA GTTCTTCCCT 
CAGTACAGCG AAGCCGAGCG CAGAATTTCC TTCTTCGCCC AGTCGCTCTC TACTCCCATC 
CCCGAGCCCC TCCCTGTTGA CAACATGCCT ACCTTCACTG TCATGATTCC TCACTACAGC 
GAAAAGATTC TTCTTTCTCT GCGTGAAATC ATTCGTGAAG ATGAGCCCTA CTCGCGTGTC 
ACTCTCCTGG AATACCTCAA GCAGCTCCAT CCCCACGAAT GGGACTGCTT CGTTAAGGAC 
ACCAAGATTC TCGCGGATGA GACCTCGCAG TTCAACGGCG AGACTGAGAA GGAGAAGGAA 
AAGGAAAAGG AGAAGGAGAC CGTCAAGAGC AAGATCGACG ATCTTCCCTT CTACTGCATC 
GGTTTCAAGT CCTCGGCACC CGAGTATACC CTCAGAACCC GTATTTGGGC CTCGCTTCGC 
TTCCAGACCT TGTACCGTAC CGTCTCGGGT TTCATGAACT ACGCTCGTGC CATTAAGCTT 
CTCTACCGTG TCGAGAACCC CGAAGTCGTC CAGATGTTTG GCGGTAACTC CGACAAGCTC 
GAGCGTGAGC TGGAGCGCAT GGCTCGTCGC AAGTTCAAGC TCTGCATTTC CATGCAGCGT 
TTCGCCAAGT TCAAGAAGGA GGAAATGGAG AACGCCGAGT TCTTGTTGCG CGCTTACCCC 
GATCTCCAAA TCGCCTACCT TGACGAAGAG CCTCCTCTTG CCGAAGGTGA GGAGCCTCGT 
CTGTACTCTG CTCTCATCGA CGGTCACTCT GAGATCATGG AGAACGGTTC GCGCCGGCCC 
AAGTTCCGTA TCCAGCTTTC CGGAAACCCC ATTCTCGGTG ACGGAAAGTC CGACAACCAG 
AACCACGCCA TCATCTTCTA CCGCGGCGAG TACATCCAGC TTATCGATGC CAACCAAGAC 
AACTATCTCG AGGAGTGCCT CAAGATCCGC AGCGTGTTGG CCGAGTTTGA GGAGATGAAG 
ACCGACAACC TTTCGCCCTA CACTCCCGGT GTCAAGAACG AGGTCCGCCA CCCTGTTGCC 
ATTCTCGGTG CTCGTGAGTA CATTTTCTCC GAGAACATTG GTATCCTCGG TGATATTGCC 
GCCGGTAAGG AACAGACATT CGGTACGCTC TTCGCCAGAA CCTTGGCCCA GATTGGTGGC 
AAGCTCCATT ACGGTCATCC TGATTTCCTC AACGGTATCT TCATGACTAC CCGTGGTGGT 
GTCTCCAAGG CTCAAAAGGG TTTGCATCTG AACGAGGATA TTTACGCTGG TATGAACGCT 
CTTCTGCGTG GTGGCCGCAT CAAGCACTGC GATTACTACC AGTGCGGTAA GGGTCGTGAT 
TTGGGTTTCG GCTCCATTCT CAACTTCACC ACCAAGATTG GAACTGGTAT GGGTGAGCAG 
CTGCTCTCTC GCGAGTACCA CTACCTTGGA ACTCAGCTCC CGATCGATCG TTTCCTGTCC 
TTCTACTACG CCCATCCCGG TTTCCACTTG AACAACATGT TCATTATGCT CTCGGTCCAG 
CTCTTCATGC TTTGCTGCGT TAACATCGGC GTACTGCGTC ATGAGACGAT CCGCTGCGAG 
TACAACCGCG AGGTCCCCAT TACCGATGCC CTCTTCCCCA CCGGCTGCTC CAACACCGAC 
GCTCTTCTGG ACTGGGTTTA CCGCTGTGTC CTCTCGATTA TCTTCGTCTT GTTCCTTGCC 
TTCGTTCCCC TGATAGTCCA GGAGATGATG GAGAAGGGTG TGATCCGCTC TGCGACTCGT 
TTCATCAAGC AGATCCTTTC GCTCTCGCCC TTCTTCGAAG TGTTCGTCTG TCAGATCTAC 
GCCAACTCTG TCCAGCAAGA TTTGTCCTTC GGTGGTGCCA GATATATCGG TACCGGTCGT 
GGTTTCGCCA CCGCGCGTAT TCCCTTTGGT GTTCTCTACT CCCGTTTCGC CGGTCCTTCG 
ATCTACTTCG GAGCCAGATT GGTCATGATG CTGCTCTTTG CCTGCCTGAC TGTCTGGCAC 
GCTGCCCTGA TCTATTTCTG GATCTCGCTT ATGGCCTTGG TCATCTCGCC CTTCCTCTAC 
AACCCTCACC AGTTCTCTTG GGGCGATTTC TTCATCGACT ACCGCGAGTA TCTCCGCTGG 
CTCTCGCGTG GTAACTCTCG CTCGCACGCC TCTTCTTGGA TCACCTTCTG CCGTCTCTCG 
CGTATTCGAA TCACTGGTTT CAAGCGCAAG ATTATCGGAG ACCCGTCGAG CAAGCTTTCG 
GGCGATGTTG CCAGGGCTGC CATCACCAAC CTGTTCTGGT CCGAGATGCT TACCCCCTTC 
ATCCTTGTCT GCTTGACGAC CATCCCGTAT CTGTTCATCA ACGCGCAGAC TGGTGTCAGG 
GGCGAATTTG GACCCCATGC CAACGACGAT AGCATCAAGC CTACCGCCTC CTTGATCCGT 
CTCTTGGTTG TCACGTTTGC GCCCATGGCT GTCAACGCCG GTGTCCTTGG TGGCTTGTTC 
GGTATGGCCT GCTGCATGGG TCCTCTTTTG AGCATGTGCT GCAAGAAGTT TGGTAGTGTT 
CTGGCCGCCA TTGCCCATGG TATGTCGGTC ATTATCCTTT TGGTTATGTT CGAAGTCATG 
TTCGTCCTGC AGAACTTTGA GTTTACCCCT ACCCTTCTCG GCATGATCGC CATGGTCTCG 
ATTCAGCGCT TCATCATCAA GATGATTGTC TCGTTGGCCT TGACTCGTGA GTTCAAGACT 
GATACCTCCA ACATTGCCTT CTGGACGGGC AAGTGGTACT CTATGGGCTG GCACTCGGTT 
TCGCAACCTG CTCGTGAGTG GCTTGCCAAG ATCACGGAGC TCAGCATGTT TGGCGCCGAC 
TTTATCCTTG GCCACCTGAT TCTGTTCTCC ATGTTCCCCG TCCTTATCAT CCCCAAGGTC 
GATGTGCTCC ACTCGGTCAT TCTCTTCTGG CTCCGTCCAA GCCGTCAAAT TCGTGCCCCC 
ATCTACTCGC TGAAGCAGAC GAAGCTCAGG AGGAGACGTG TGATCCGTTA TGCGATGCTC 
TACTTTGTCC TGCTTGTCAT CTTTGTCGCC CTGATTGTTG GACCGATTGT GGCCGGCAAA 
CAGATTCCTC CTGGCACGCT CGACATGGTC AACAATTTTG GCGGTGGAAG CATGATCCTC 
AAGCAGTACC CCTGGAAGCT GGGACCTTCT GGTGACAATG ACGACACCAG AGGTCGTCTG 
GAAACGGGAA CCAAGGCTAC TAACTACTCT GGTGTCTGGA CCCCGACGAC AACATCGGAC 
TATGTCGCCA AGGCTACTAG CAAGGCTGAA AGGAGGATGG CATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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do cDNA ORF clone, Neurospora crassa OR74A

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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