bgs2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	bgs2
Entrez Gene ID	2541586
Full Name	1,3-beta-glucan synthase subunit Bgs2
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019457.2	NP_594032.1	1,3-beta-glucan synthase subunit Bgs2

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACL181C	NP_983223.2
Magnaporthe oryzae (rice blast fungus)	MGG_00865	XP_003718107.1
Schizosaccharomyces pombe (fission yeast)	bgs2	NP_594032.1
Schizosaccharomyces pombe (fission yeast)	bgs4	NP_588501.1
Neurospora crassa	NCU06871	XP_957980.1
Kluyveromyces lactis	KLLA0B05841g	XP_451796.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bgs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bgs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101946	NM_001019457.2 Latest version!	Schizosaccharomyces pombe 1,3-beta-glucan synthase subunit Bgs2 (bgs2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bgs2 ( NM_001019457.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594032.1 Schizosaccharomyces pombe 1,3-beta-glucan synthase subunit Bgs2 (bgs2), partial mRNA.

Price & Availability↑

CloneID	OSc101946
Clone ID Related Accession (Same CDS sequence)	NM_001019457.2
Accession Version	NM_001019457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	1,3-beta-glucan synthase subunit Bgs2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019457.1. COMPLETENESS: incomplete on the 5' end.

ATGTCATGGC ATGAACAAGA TTACGGCTTT GGTACTTCAT CTGAAAACTC TAAAATAAAT 
TCGGATGAAT TTGAGGACTC AATGGATGTC ACGGAATTCA ATAATCCAGG AGAAGAGAGT 
ACATATCCAC AAGCCAATTC ATGGAACGAC TCAAATAAAA CCAAGGACAT TGCAGAATAT 
TATGATTACA GCTGGTCAGG ACAAAGAGAA GCCAATCAAC AGATTCCCCA ACCAGTACCC 
GTACAGCCCC GATATCCTGA TGAACAAAAT TTCTCAATGA ACGGAGAAAC GTATCCAAGT 
GAGGCATATG ATTACGACTA TTCCGGCCAA GAAGAAGCAA TTAATGCTAT GAATATACTT 
CAAGGGAACT TGGAAAGGAG TAACGAATAT TATTCAGAAG GTGTTCCTTA CAGCGAGGAA 
GACTTGGGTG CATATGCTGG AGGAATGACA GAAGACGATC AGATGTATTC AAATTTCAAC 
GAAACCGGGG AATTTAATTT AGATATAGGC TCTTCAAAGT TTTCGTATCT GAATGCAAAA 
AATCCGTACC CTGCATGGAT TGCTGAAAAC TCGATCCCCA TTACCGCCGA AAATATACTT 
GAGATTTTTC AAGAGCTTCA GGCAAAATTT GGTTTCCAGT ATGATTCAAT GCTCAATATG 
TACGACTTTT TTATGGTTTT ATTGGACTCG AGATCTTCAC GTATGGATGC TGAGAATGCT 
TTAAAATCTC TGCATGCTGA TTACATTGGA GGTAGGAATG CCAATTATAG AAAATGGTAT 
TTCTCATCAA GTATGGATAT TGATGATTCT GTCGGCTTGC AAAATTGCAA GTTTTCAAGT 
AATGGGCCAA AAATCAAACA AGCAAAGAAA AAGCAAAAAC GAAAGTCCAA CAAGGCCGAG 
ACAGAAGGAA CAAATGAACC GGAAACTTCC GTGCAAATCG ATCCATTGAA TGTTTCAATG 
GAGAATTGGG AAAACGAAAT GAAAAATCTG GATTGCGAAA CTCAGGTTCG TCAGCTTGCA 
CTTTACCTTT TGTGCTGGGG TGAAGCCAAC AACATTAGAT TTTGTCCCGA ATGCCTTTGT 
TTCATATTCA AGCTTGCAAA TGACTTTATG CAATCGGAGG ATTATGCGAA AAGTGAACCC 
ATTGAAGACG ATTGCTTTTA TCTAGACAAT GTAATTACAC CATTGTACGA ATTCATTCGC 
GATCAGCAAT TTGAGCTTCT TGATGGAAAA TTAGTTCGTA GAGAAAGGGA CCACGCTCAA 
ATAATAGGTT ACGATGACAT TAATCAACTG TTTTGGTACC CAGAGGGGAT AGCCCGAATA 
GTTACGGTGG ATGGGACACA ACTCATCACA CTACCAAAAT GGGAAAGGTT TCACAAGTTA 
AGTGAAGTGG ATTGGAAAAA GGCTTTTTAT AAAACTTTTT ATGAGAGTCG CTCGTGGTTT 
CATTTAGTTA CCAATTTTAA TCGAATATGG GTCATACATT TCACTACTTA TTGGTACTAC 
ACAGTCTTTA ATTCACCAAC TATAATTGAG AAGAATTTTC GGCAATCAGT TGGTCCTAAA 
CCAATTCCGT CATGCCATTG GACTTCTGTC TCCCTTGGAG GAGCTGTTGC AACTTTGTTG 
ATGCTATTAG CAACTATTTT TGAATGGATT CATGTACCTC GAAAGTTTCC AGGATCTAGG 
CCTCTATTAA AACGCTTCCT AATTCTCATT TTGTTCTTCA TCTTAAATGT CGCTCCAACT 
GTTTTTGTTT TTGGATTTTC AACTGAAGAA CAGCAGCGTA CTACTGGAAG ACTAACTGTC 
GCAATTGTTC ATTTCATTTT CTCTGTATTT ACATTCATTT ACTTTTCCCT TGTGCCTTTA 
AACAATCTGT TTCATCGTGC TTACAAATCT TCCTCACGCA CGCATTTAGC TAATAGATAC 
TTTACTGCCG ATTATGCTCG TCTCCAAATA AACGATATGT GTGTATCATG GGGACTTTGG 
CTGCTGGTGT TTGGAGCAAA ATTTACGGAA TCGTACTTTT TCCTTTCCCT GTCATTTCGA 
GACCCCATCC TCGTATTGAG TACAATGAAG CCCTATCTAT GCAATATAAC GTTTCTAGGC 
TCTCATCTAT GCATATGGCA ACCAAAGATT CTACTTGGTA TCATGTATGT CACTGACCTA 
GTACTGTTTT TCCTCGACAC GTATTTATGG TACATTTTAG TCAACACAGT TTTTTCGGTT 
GCAAGGTCAT TTTTCTTGGG AATTTCAATA TGGACACCTT GGAGAAATAT TTTTGCTAGG 
ATGCCAAAAA GAATTTACAG CAAAATTCTT TGTACCCCTG AAGTTGATTC GAGTTATAAG 
CCTAAAGTTC TTGTATCTCA GATTTGGAAC TCAATTATTA TCTCACTGTA TAGAGAGCAT 
TTATTGGCCA TTGAACATGT TCAACGTCTT ATTTATCATC AAGTTAATTC TCTTGATGGG 
GATGGAAGTA AAACATTAAA AACACCAACT TTCTTCGTAT CCCAGGAAGA TTCTTCGTTT 
AATACCGAAT ATTTCCCTGC TCATTCGGAG GCCGAAAGGC GACTTTCGTT TTTTGCTCAA 
TCTTTAGCAA CACCAATTCC AGAACCCATT CCAGTGGATG CGATGCCTAC TTTTACGGTA 
TTGGTGCCGC ATTATGGTGA AAAGATACTG CTTTCATTGA AAGAAATTAT TCGAGAGCAA 
GATAAACTTT CTCGGGTTAC TTTACTTGAG TACCTTAAGC AGTTGCACGC TAATGAATGG 
AAATGTTTCG TTCGCGACAC TAAAATCCTT GCTGAAGAAG ATGCGCTTTC CAATCAAGAC 
TTGAACTCAC AAGATGAGTC AATGAAGGCC GAGCAGCTTC ACAAAAAATT CGACGATTTA 
CCATTTTATT GTATAGGTTT CAAAAACGCT ACTCCTGAGT ATACTCTTCG TACTAGAATT 
TGGGCTTCAT TAAGGAGTCA AACCTTGTAC CGAACCGTTA GTGGTTTTAT GAATTATTCA 
CGCGCTATTA AACTGCTTTA TAGAGTTGAA AATCCCGATG TTGCACAATT GTTTGAAGGG 
CAGATGGATG TTCTCGAATA TGAACTAGAT CGAATGGCAT CTCGGAAATT TAAAATGTGC 
GTATCGATGC AGAGGTACGC AAAGTTCACT GCTGATGAGA TAGAGAACAC GGAGTTCATT 
TTACGTGCAT ACCCTGATTT ATTGATCGCC TATTTAGACG AAGATCCTCC AAAGGAGGGT 
GAAACAACAC CTCAATTGTA TGCAGCACTG ATTGACGGAT ATTCTGAATT AGATGAAAAT 
AAGAAACGAA AACCTAAATA CCGCATCAAA CTCTCTGGAA ATCCAATCCT CGGTGACGGT 
AAGTCTGATA ACCAAAATTT ATCTCTTCCT TTTTACAGAG GTGAATATAT TCAACTGATT 
GATGCCAACC AGGATAATTA TTTGGAGGAG TGTTTAAAAA TAAGAAGTAT TTTGGCTGAA 
TTTGAAGCTT TTGATTTAAA AACAAATGAC CCCTATGCTG AAACTAATGC ACTTTACCAG 
AATAATCCAG TTGCTATCAT GGGTGCAAGA GAGTATATAT TTTCTGAAAA TATAGGAATT 
TTGGGAGATG TTGCTGCTGG TAAAGAACAA ACATTTGGAA CATTATTTGC AAGAACGATG 
GCTCAAATCG GTGGTAAGTT GCATTATGGG CACCCTGATT TTTTAAATGC CATTTACATG 
ACAACACGTG GCGGTGTCTC CAAAGCACAA AAAGGTTTAC ATGTGAACGA AGACATATAT 
GCTGGAATGA CTGCTCTTCA GAGAGGCGGA CGAATTAAGC ATTGTGAGTA TTACCAATGC 
GGAAAGGGTC GCGATTTAGG TTTTGGCTCA ATTCTCAATT TCACTACAAA AATTGGTACT 
GGTATGGGAG AGCAGATGGT GTCTCGTGAA TATTATTACT TAGGAACTCA ACTACCATTT 
GACAGATTTT TATCCTTCTA TTACGCACAT CCTGGTTTTC ATATTAACAA TATTTTCATC 
ATGCTATCCG TTCAACTATT CATGGTTGTT TTGGTGAATC TCGGAGGAAT GTATCATGTG 
GTAACAGTTT GCGATTACGA TCATGATCAA AAGCTTACTG TTCCAATGAG GCCAGAAGGA 
TGTTATCAAC TTAATCCAGT AGTAAATTGG TTGAAGAGAT GCATTATATC TATTTTCATT 
GTTTTTTTCA TCTCATTTGT TCCATTGACA GTTCAGGAAT TGACTGAAAG AGGTGCATGG 
CGTGCCCTAA CTCGTTTAGG GAAGCACTTT GCATCGTTTT CCCCTATGTT TGAAGTATTC 
GCTTGCCAAA CATATGCACA GTCGGTAATT GCTAATCTTT CATTTGGTGG TGCACGGTAT 
ATTGGAACGG GCAGAGGATT TGCTACAGCA AGGCTTTCTT TTTCGTTATT GTTTTCGAGG 
TTTGCGGGTC CTTCTATATA CCTTGGGTCA CGGACGTTGT TGATGCTGTT GTTTGGAACA 
ATGACCGTCT GGATACCTCA CTTGATATAT TTTTGGATAT CTACGCTAGC CATGTGCATC 
AGTCCCTTTA TATTTAATCC ACATCAATTT TCGTGGACTG ACTTCTTTGT TGATTACCGC 
GAATTCATTC GATGGCTTTC AAGGGGTAAT TCACGTTCTC ATATAAACTC ATGGATAGGC 
TATTGTCGTT TAACCCGAAC TCGAATAACA GGTTACAAAA GACGACTGCT TGGGGTTCCA 
GTTAGCAAGG GTGTTATCGA TACATCGAGG GCACATTTTA CGAATATGTT TTTTACGGAA 
ATATTTATTC CACTAATGCT GGTTCCTTTG ACACTTGTCT CTTATTTTTT TATAGATTCA 
CAGCCTGGTA ATCCTGATCC CTCGAAAGTT ACCAATCCGA TTCTTCGTAT ATTAATTTTG 
GCCTTTCTTC CAATTATAGT TGCCGCCGTA GTTTCTATGA CATTTGCTGG AATGGCTTGT 
ATGATGGGAC CTCTGTTAGA TCTTTGTTGC AAAAAATTTG GAGCTGTACT AGCAGCACTG 
GCTCATGGTA TTACTGTGTT TATGTTTATT ATCGTCTTTG AGGTATCTTG GTATTTAGAG 
GCTTGGTGCC TTGCGAAAAC GGTGCTTAGT ATGCTGTGCA TAATTGCGAT TCAACGATTC 
TTTTTTAAGA TCATTCAAGT TTTATTTTTA ACAAGGGAGT TGAAGCACGA TGGAACGAAC 
CTGGCATGGT GGACGGGAAA ATGGTATTCC AGAGGACTGG GATTTCACGC GTTGTCCCAG 
CCGTCACGAG AACTTATTTG CAAAATGACT GAACTAAATT TTTTTGCAGC TGATTTCTTT 
CTTTGTCATT TACTTTTATT TCTGATGTTA CCCGTACTTT TGATTCCGTT TATAGATAGA 
TGGCATTCGA TGATGCTTTT TTGGTTGAAA CCAAGCAAAC AAATTCGACC ACCCATATAT 
TCGATGCGGC AAAACCGTTT ACGTAAGAAG ATCGTTCAAC GCTACGGAAC TATGTTCTTT 
CTTTTGTTGA TTGCATTTTT AGCGCTCATC ATAATTCCTT TAGTAGTAGC CAAGGATTTA 
CTAGCCAACT TCGAAATGGA CATCCTTCGA ACGTATGGTC TAATGCAGCC GCGAGATACA 
AATTGGACGG AGAATGGAAC GAATTGGACC ACTGTCAATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594032.1
CDS	1..5685
Translation	MSWHEQDYGFGTSSENSKINSDEFEDSMDVTEFNNPGEESTYPQANSWNDSNKTKDIAEYYDYSWSGQREANQQIPQPVPVQPRYPDEQNFSMNGETYPSEAYDYDYSGQEEAINAMNILQGNLERSNEYYSEGVPYSEEDLGAYAGGMTEDDQMYSNFNETGEFNLDIGSSKFSYLNAKNPYPAWIAENSIPITAENILEIFQELQAKFGFQYDSMLNMYDFFMVLLDSRSSRMDAENALKSLHADYIGGRNANYRKWYFSSSMDIDDSVGLQNCKFSSNGPKIKQAKKKQKRKSNKAETEGTNEPETSVQIDPLNVSMENWENEMKNLDCETQVRQLALYLLCWGEANNIRFCPECLCFIFKLANDFMQSEDYAKSEPIEDDCFYLDNVITPLYEFIRDQQFELLDGKLVRRERDHAQIIGYDDINQLFWYPEGIARIVTVDGTQLITLPKWERFHKLSEVDWKKAFYKTFYESRSWFHLVTNFNRIWVIHFTTYWYYTVFNSPTIIEKNFRQSVGPKPIPSCHWTSVSLGGAVATLLMLLATIFEWIHVPRKFPGSRPLLKRFLILILFFILNVAPTVFVFGFSTEEQQRTTGRLTVAIVHFIFSVFTFIYFSLVPLNNLFHRAYKSSSRTHLANRYFTADYARLQINDMCVSWGLWLLVFGAKFTESYFFLSLSFRDPILVLSTMKPYLCNITFLGSHLCIWQPKILLGIMYVTDLVLFFLDTYLWYILVNTVFSVARSFFLGISIWTPWRNIFARMPKRIYSKILCTPEVDSSYKPKVLVSQIWNSIIISLYREHLLAIEHVQRLIYHQVNSLDGDGSKTLKTPTFFVSQEDSSFNTEYFPAHSEAERRLSFFAQSLATPIPEPIPVDAMPTFTVLVPHYGEKILLSLKEIIREQDKLSRVTLLEYLKQLHANEWKCFVRDTKILAEEDALSNQDLNSQDESMKAEQLHKKFDDLPFYCIGFKNATPEYTLRTRIWASLRSQTLYRTVSGFMNYSRAIKLLYRVENPDVAQLFEGQMDVLEYELDRMASRKFKMCVSMQRYAKFTADEIENTEFILRAYPDLLIAYLDEDPPKEGETTPQLYAALIDGYSELDENKKRKPKYRIKLSGNPILGDGKSDNQNLSLPFYRGEYIQLIDANQDNYLEECLKIRSILAEFEAFDLKTNDPYAETNALYQNNPVAIMGAREYIFSENIGILGDVAAGKEQTFGTLFARTMAQIGGKLHYGHPDFLNAIYMTTRGGVSKAQKGLHVNEDIYAGMTALQRGGRIKHCEYYQCGKGRDLGFGSILNFTTKIGTGMGEQMVSREYYYLGTQLPFDRFLSFYYAHPGFHINNIFIMLSVQLFMVVLVNLGGMYHVVTVCDYDHDQKLTVPMRPEGCYQLNPVVNWLKRCIISIFIVFFISFVPLTVQELTERGAWRALTRLGKHFASFSPMFEVFACQTYAQSVIANLSFGGARYIGTGRGFATARLSFSLLFSRFAGPSIYLGSRTLLMLLFGTMTVWIPHLIYFWISTLAMCISPFIFNPHQFSWTDFFVDYREFIRWLSRGNSRSHINSWIGYCRLTRTRITGYKRRLLGVPVSKGVIDTSRAHFTNMFFTEIFIPLMLVPLTLVSYFFIDSQPGNPDPSKVTNPILRILILAFLPIIVAAVVSMTFAGMACMMGPLLDLCCKKFGAVLAALAHGITVFMFIIVFEVSWYLEAWCLAKTVLSMLCIIAIQRFFFKIIQVLFLTRELKHDGTNLAWWTGKWYSRGLGFHALSQPSRELICKMTELNFFAADFFLCHLLLFLMLPVLLIPFIDRWHSMMLFWLKPSKQIRPPIYSMRQNRLRKKIVQRYGTMFFLLLIAFLALIIIPLVVAKDLLANFEMDILRTYGLMQPRDTNWTENGTNWTTVNE

Target ORF information:

RefSeq Version	NM_001019457.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe 1,3-beta-glucan synthase subunit Bgs2 (bgs2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019457.2

ORF Insert Sequence:

ATGTCATGGC ATGAACAAGA TTACGGCTTT GGTACTTCAT CTGAAAACTC TAAAATAAAT 
TCGGATGAAT TTGAGGACTC AATGGATGTC ACGGAATTCA ATAATCCAGG AGAAGAGAGT 
ACATATCCAC AAGCCAATTC ATGGAACGAC TCAAATAAAA CCAAGGACAT TGCAGAATAT 
TATGATTACA GCTGGTCAGG ACAAAGAGAA GCCAATCAAC AGATTCCCCA ACCAGTACCC 
GTACAGCCCC GATATCCTGA TGAACAAAAT TTCTCAATGA ACGGAGAAAC GTATCCAAGT 
GAGGCATATG ATTACGACTA TTCCGGCCAA GAAGAAGCAA TTAATGCTAT GAATATACTT 
CAAGGGAACT TGGAAAGGAG TAACGAATAT TATTCAGAAG GTGTTCCTTA CAGCGAGGAA 
GACTTGGGTG CATATGCTGG AGGAATGACA GAAGACGATC AGATGTATTC AAATTTCAAC 
GAAACCGGGG AATTTAATTT AGATATAGGC TCTTCAAAGT TTTCGTATCT GAATGCAAAA 
AATCCGTACC CTGCATGGAT TGCTGAAAAC TCGATCCCCA TTACCGCCGA AAATATACTT 
GAGATTTTTC AAGAGCTTCA GGCAAAATTT GGTTTCCAGT ATGATTCAAT GCTCAATATG 
TACGACTTTT TTATGGTTTT ATTGGACTCG AGATCTTCAC GTATGGATGC TGAGAATGCT 
TTAAAATCTC TGCATGCTGA TTACATTGGA GGTAGGAATG CCAATTATAG AAAATGGTAT 
TTCTCATCAA GTATGGATAT TGATGATTCT GTCGGCTTGC AAAATTGCAA GTTTTCAAGT 
AATGGGCCAA AAATCAAACA AGCAAAGAAA AAGCAAAAAC GAAAGTCCAA CAAGGCCGAG 
ACAGAAGGAA CAAATGAACC GGAAACTTCC GTGCAAATCG ATCCATTGAA TGTTTCAATG 
GAGAATTGGG AAAACGAAAT GAAAAATCTG GATTGCGAAA CTCAGGTTCG TCAGCTTGCA 
CTTTACCTTT TGTGCTGGGG TGAAGCCAAC AACATTAGAT TTTGTCCCGA ATGCCTTTGT 
TTCATATTCA AGCTTGCAAA TGACTTTATG CAATCGGAGG ATTATGCGAA AAGTGAACCC 
ATTGAAGACG ATTGCTTTTA TCTAGACAAT GTAATTACAC CATTGTACGA ATTCATTCGC 
GATCAGCAAT TTGAGCTTCT TGATGGAAAA TTAGTTCGTA GAGAAAGGGA CCACGCTCAA 
ATAATAGGTT ACGATGACAT TAATCAACTG TTTTGGTACC CAGAGGGGAT AGCCCGAATA 
GTTACGGTGG ATGGGACACA ACTCATCACA CTACCAAAAT GGGAAAGGTT TCACAAGTTA 
AGTGAAGTGG ATTGGAAAAA GGCTTTTTAT AAAACTTTTT ATGAGAGTCG CTCGTGGTTT 
CATTTAGTTA CCAATTTTAA TCGAATATGG GTCATACATT TCACTACTTA TTGGTACTAC 
ACAGTCTTTA ATTCACCAAC TATAATTGAG AAGAATTTTC GGCAATCAGT TGGTCCTAAA 
CCAATTCCGT CATGCCATTG GACTTCTGTC TCCCTTGGAG GAGCTGTTGC AACTTTGTTG 
ATGCTATTAG CAACTATTTT TGAATGGATT CATGTACCTC GAAAGTTTCC AGGATCTAGG 
CCTCTATTAA AACGCTTCCT AATTCTCATT TTGTTCTTCA TCTTAAATGT CGCTCCAACT 
GTTTTTGTTT TTGGATTTTC AACTGAAGAA CAGCAGCGTA CTACTGGAAG ACTAACTGTC 
GCAATTGTTC ATTTCATTTT CTCTGTATTT ACATTCATTT ACTTTTCCCT TGTGCCTTTA 
AACAATCTGT TTCATCGTGC TTACAAATCT TCCTCACGCA CGCATTTAGC TAATAGATAC 
TTTACTGCCG ATTATGCTCG TCTCCAAATA AACGATATGT GTGTATCATG GGGACTTTGG 
CTGCTGGTGT TTGGAGCAAA ATTTACGGAA TCGTACTTTT TCCTTTCCCT GTCATTTCGA 
GACCCCATCC TCGTATTGAG TACAATGAAG CCCTATCTAT GCAATATAAC GTTTCTAGGC 
TCTCATCTAT GCATATGGCA ACCAAAGATT CTACTTGGTA TCATGTATGT CACTGACCTA 
GTACTGTTTT TCCTCGACAC GTATTTATGG TACATTTTAG TCAACACAGT TTTTTCGGTT 
GCAAGGTCAT TTTTCTTGGG AATTTCAATA TGGACACCTT GGAGAAATAT TTTTGCTAGG 
ATGCCAAAAA GAATTTACAG CAAAATTCTT TGTACCCCTG AAGTTGATTC GAGTTATAAG 
CCTAAAGTTC TTGTATCTCA GATTTGGAAC TCAATTATTA TCTCACTGTA TAGAGAGCAT 
TTATTGGCCA TTGAACATGT TCAACGTCTT ATTTATCATC AAGTTAATTC TCTTGATGGG 
GATGGAAGTA AAACATTAAA AACACCAACT TTCTTCGTAT CCCAGGAAGA TTCTTCGTTT 
AATACCGAAT ATTTCCCTGC TCATTCGGAG GCCGAAAGGC GACTTTCGTT TTTTGCTCAA 
TCTTTAGCAA CACCAATTCC AGAACCCATT CCAGTGGATG CGATGCCTAC TTTTACGGTA 
TTGGTGCCGC ATTATGGTGA AAAGATACTG CTTTCATTGA AAGAAATTAT TCGAGAGCAA 
GATAAACTTT CTCGGGTTAC TTTACTTGAG TACCTTAAGC AGTTGCACGC TAATGAATGG 
AAATGTTTCG TTCGCGACAC TAAAATCCTT GCTGAAGAAG ATGCGCTTTC CAATCAAGAC 
TTGAACTCAC AAGATGAGTC AATGAAGGCC GAGCAGCTTC ACAAAAAATT CGACGATTTA 
CCATTTTATT GTATAGGTTT CAAAAACGCT ACTCCTGAGT ATACTCTTCG TACTAGAATT 
TGGGCTTCAT TAAGGAGTCA AACCTTGTAC CGAACCGTTA GTGGTTTTAT GAATTATTCA 
CGCGCTATTA AACTGCTTTA TAGAGTTGAA AATCCCGATG TTGCACAATT GTTTGAAGGG 
CAGATGGATG TTCTCGAATA TGAACTAGAT CGAATGGCAT CTCGGAAATT TAAAATGTGC 
GTATCGATGC AGAGGTACGC AAAGTTCACT GCTGATGAGA TAGAGAACAC GGAGTTCATT 
TTACGTGCAT ACCCTGATTT ATTGATCGCC TATTTAGACG AAGATCCTCC AAAGGAGGGT 
GAAACAACAC CTCAATTGTA TGCAGCACTG ATTGACGGAT ATTCTGAATT AGATGAAAAT 
AAGAAACGAA AACCTAAATA CCGCATCAAA CTCTCTGGAA ATCCAATCCT CGGTGACGGT 
AAGTCTGATA ACCAAAATTT ATCTCTTCCT TTTTACAGAG GTGAATATAT TCAACTGATT 
GATGCCAACC AGGATAATTA TTTGGAGGAG TGTTTAAAAA TAAGAAGTAT TTTGGCTGAA 
TTTGAAGCTT TTGATTTAAA AACAAATGAC CCCTATGCTG AAACTAATGC ACTTTACCAG 
AATAATCCAG TTGCTATCAT GGGTGCAAGA GAGTATATAT TTTCTGAAAA TATAGGAATT 
TTGGGAGATG TTGCTGCTGG TAAAGAACAA ACATTTGGAA CATTATTTGC AAGAACGATG 
GCTCAAATCG GTGGTAAGTT GCATTATGGG CACCCTGATT TTTTAAATGC CATTTACATG 
ACAACACGTG GCGGTGTCTC CAAAGCACAA AAAGGTTTAC ATGTGAACGA AGACATATAT 
GCTGGAATGA CTGCTCTTCA GAGAGGCGGA CGAATTAAGC ATTGTGAGTA TTACCAATGC 
GGAAAGGGTC GCGATTTAGG TTTTGGCTCA ATTCTCAATT TCACTACAAA AATTGGTACT 
GGTATGGGAG AGCAGATGGT GTCTCGTGAA TATTATTACT TAGGAACTCA ACTACCATTT 
GACAGATTTT TATCCTTCTA TTACGCACAT CCTGGTTTTC ATATTAACAA TATTTTCATC 
ATGCTATCCG TTCAACTATT CATGGTTGTT TTGGTGAATC TCGGAGGAAT GTATCATGTG 
GTAACAGTTT GCGATTACGA TCATGATCAA AAGCTTACTG TTCCAATGAG GCCAGAAGGA 
TGTTATCAAC TTAATCCAGT AGTAAATTGG TTGAAGAGAT GCATTATATC TATTTTCATT 
GTTTTTTTCA TCTCATTTGT TCCATTGACA GTTCAGGAAT TGACTGAAAG AGGTGCATGG 
CGTGCCCTAA CTCGTTTAGG GAAGCACTTT GCATCGTTTT CCCCTATGTT TGAAGTATTC 
GCTTGCCAAA CATATGCACA GTCGGTAATT GCTAATCTTT CATTTGGTGG TGCACGGTAT 
ATTGGAACGG GCAGAGGATT TGCTACAGCA AGGCTTTCTT TTTCGTTATT GTTTTCGAGG 
TTTGCGGGTC CTTCTATATA CCTTGGGTCA CGGACGTTGT TGATGCTGTT GTTTGGAACA 
ATGACCGTCT GGATACCTCA CTTGATATAT TTTTGGATAT CTACGCTAGC CATGTGCATC 
AGTCCCTTTA TATTTAATCC ACATCAATTT TCGTGGACTG ACTTCTTTGT TGATTACCGC 
GAATTCATTC GATGGCTTTC AAGGGGTAAT TCACGTTCTC ATATAAACTC ATGGATAGGC 
TATTGTCGTT TAACCCGAAC TCGAATAACA GGTTACAAAA GACGACTGCT TGGGGTTCCA 
GTTAGCAAGG GTGTTATCGA TACATCGAGG GCACATTTTA CGAATATGTT TTTTACGGAA 
ATATTTATTC CACTAATGCT GGTTCCTTTG ACACTTGTCT CTTATTTTTT TATAGATTCA 
CAGCCTGGTA ATCCTGATCC CTCGAAAGTT ACCAATCCGA TTCTTCGTAT ATTAATTTTG 
GCCTTTCTTC CAATTATAGT TGCCGCCGTA GTTTCTATGA CATTTGCTGG AATGGCTTGT 
ATGATGGGAC CTCTGTTAGA TCTTTGTTGC AAAAAATTTG GAGCTGTACT AGCAGCACTG 
GCTCATGGTA TTACTGTGTT TATGTTTATT ATCGTCTTTG AGGTATCTTG GTATTTAGAG 
GCTTGGTGCC TTGCGAAAAC GGTGCTTAGT ATGCTGTGCA TAATTGCGAT TCAACGATTC 
TTTTTTAAGA TCATTCAAGT TTTATTTTTA ACAAGGGAGT TGAAGCACGA TGGAACGAAC 
CTGGCATGGT GGACGGGAAA ATGGTATTCC AGAGGACTGG GATTTCACGC GTTGTCCCAG 
CCGTCACGAG AACTTATTTG CAAAATGACT GAACTAAATT TTTTTGCAGC TGATTTCTTT 
CTTTGTCATT TACTTTTATT TCTGATGTTA CCCGTACTTT TGATTCCGTT TATAGATAGA 
TGGCATTCGA TGATGCTTTT TTGGTTGAAA CCAAGCAAAC AAATTCGACC ACCCATATAT 
TCGATGCGGC AAAACCGTTT ACGTAAGAAG ATCGTTCAAC GCTACGGAAC TATGTTCTTT 
CTTTTGTTGA TTGCATTTTT AGCGCTCATC ATAATTCCTT TAGTAGTAGC CAAGGATTTA 
CTAGCCAACT TCGAAATGGA CATCCTTCGA ACGTATGGTC TAATGCAGCC GCGAGATACA 
AATTGGACGG AGAATGGAAC GAATTGGACC ACTGTCAATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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bgs2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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