bgs4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	bgs4
Entrez Gene ID	2538822
Full Name	1,3-beta-glucan synthase subunit Bgs4
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023491.2	NP_588501.1	1,3-beta-glucan synthase subunit Bgs4

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACL181C	NP_983223.2
Magnaporthe oryzae (rice blast fungus)	MGG_00865	XP_003718107.1
Schizosaccharomyces pombe (fission yeast)	bgs2	NP_594032.1
Schizosaccharomyces pombe (fission yeast)	bgs4	NP_588501.1
Neurospora crassa	NCU06871	XP_957980.1
Kluyveromyces lactis	KLLA0B05841g	XP_451796.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bgs4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bgs4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99292	NM_001023491.2 Latest version!	Schizosaccharomyces pombe 1,3-beta-glucan synthase subunit Bgs4 (bgs4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bgs4 ( NM_001023491.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588501.1 Schizosaccharomyces pombe 1,3-beta-glucan synthase subunit Bgs4 (bgs4), mRNA.

Price & Availability↑

CloneID	OSc99292
Clone ID Related Accession (Same CDS sequence)	NM_001023491.2
Accession Version	NM_001023491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	1,3-beta-glucan synthase subunit Bgs4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023491.1.

ATGTCTGGAA ATAATGAAAA TGTTTCAGGT ATTACTGGTC ATGATGCTGT CTCGGATGAT 
CAGTACGCTT ACGATTCAGA GGTTTATGAT GACCAAAATG CATACCAACG CCAGCCTGCT 
GATGCGTATT CCAATGAGGA GTTTTTGGAT CAGGCTGACT ACGATTCTAT GTATGGAGAG 
GGTTACAATG GGTATGATTA CCCTACTGGT GTAACTGAAA GTTATGGAGA TGAATACACT 
CCTGTCGACA CCGCCTCTTC AGGTATCAAT CAATATTCCA CTGAAAAAGG AAAATTTACT 
CGTCCTTCAG ATGAATACGA GTCTGAGTAT TCAGATTACA ATGCTCAGCC CTCAGATGCC 
AACAATTTTT ACAATTTGAG AGGCGATGGT AGATACAACG CTTATGATCC TAGTTCCGAT 
TCGTTGGCTA ACGTCTACAA CAGTGTGCCT TACGGTAGCA GTCCGTATGA TTTTAGCAAC 
TCTTCTTTTG TTGGTAATTC GGGCTCAGGT ACCCCATTGG ATGGCGATAG TGGTTCATTC 
TATGCCGATA GCGCTAATCT CACAAATCGT GAGCCTTATC CTGCCTGGAC TCCTGAAAAC 
GAACTCCCTT TAACTAAGGA AGAGATTGAA GACATATTCA TTGATCTAAC CAATAAATTG 
GGTTTCCAAC GCGATTCCAT GCGTAATATG TATGATTTTT TTATGTGTCT TTTAGATTCT 
CGTGCTTCTC GCATGACTCC AGATCAAGCA CTGCTTACGC TTCATGCCGA TTATATTGGT 
AGTGATATTG CTAACTATAA AAAATGGTAT TTTGCTTCTC AGATGGATCG GGAAGATGCA 
GTCGGTCTTG CTAATGTTGG CATTTATGGT GGTAAAGTTA CTTCTATCAA AGAGAAAGGA 
AAGTTCTTTT CTCGAAACAA AAAAGCCCCT AAAGTTGTTA AGCCTCCAAG GAAGTCTCGC 
TTCAAGCGCA AGAAGAAAAA GGAGCAACCC GAGGAGGCGG AAGACGAATA CATTGATGTT 
AATACCGATG ATTCTTTAGA ATCCGCCGAA TATCGTTGGA GATCTCACAT GCGCTCAATG 
ACTCAATTCG AGCGTGCTCA ACAAATTGCA TTGTGGCTTT TGTTGTGGGG TGAGGCAAAC 
AATGTTCGTT TCATGCCTGA GGTAATAGCC TTCCTTTTCA AATGTGCTTA CGATTATATT 
ATTTCACCTG AGGCACAAAA CGTCACTGAA CCAGTTCCTG AAGGATATTA CTTGGATAAT 
ATTGTTTCCC CACTCTACCA ATATATGCAC GATCAGCAGT TTGAAATTAT CAATGGGAAA 
TATGTACGTC GGGAACGTCC ACATGACCAG TTAATTGGAT ACGATGATAT TAACCAACTT 
TTCTGGCATG CTGAGGGTAT AGCACGTCTT ATTTTTGAAG ACGGCACTCG TCTTATTGAT 
ATTCCTGCTT CTGAGCGTTT TCATAGGCTT CCAGAAGTTC AATGGAATCG TGCTTTTTAT 
AAAACTTATT ATGAATCCCG TTCTTGGTTT CATCTTATTA CCAATTTCAA TCGTATTTGG 
GTCATCCACT TTGGTATGTT TTGGTATTTT ACTGCTTTCA ACTCTCCTAC TCTTTACACA 
AAACCTTTCC ATCAGCGCGA TGGTCCCAAG CCAACAGGTG CTTCCCAATG GGCTGCTGTT 
GCTTGTACCT CTGTTGTTTC TTGTATTATC ATGGCTGCTG CTTCTTTGTG TGAATATCTT 
TTTGTCCCCC GTCGTTTCCC TGGATCAAAA CCCATTTGGA AGCGTTTATG TATCATTGTT 
TTAATCGCTA TCATTAACTT GATTCCTATC GTCTATATCT TCGGTTTCTC AAGTAAGCAT 
CAACAGCGTA GTGGTCGACG TATCGCTGTT GGTGTAGTTG CATTTTTGAT GTCTATTGCT 
ACATACGTCT ACTTTTCTCT TGTACCTCTA CAAAGTACAT TTGGAAAATT GTCCGTGAAA 
GATTCTCGTA AATACTTGGC AAACAAGTAT TTCACTTCAA ATTTTGCACC ATTAAAATTT 
GATAACCAAG CACTCTCTGT TATTATTTGG GTCTGTGTTT TCACATGCAA ATTTGCTGAA 
TCTTATTTCT TCCTTACCTT GTCTATCAGA GATCCTATTA TTGTTTTGAG TACCATGCGA 
CCTTATCTTT GCTCTATTTA TTGGGCGGGT AGCAGATTAT GTTTTGTCCA ACCAAGGATC 
ATTTTGGGTA TCATGTATTT TACTGACTTG ATTCTCTTCT TTTTGGATAC GTACCTTTGG 
TATATTATTT TCAATACTAT CTTCTCTGTT TTAAGATCAT TCGTCTTAGG TATTTCGATC 
CTCACACCAT GGAGAAATAT CTTTAGCCGT ATGCCCCAAA GAATTTATGG TAAAATTTTG 
GCCACGAATG ACATGGAAAT TAAGTACAAA CCTAAAATTT TAATTTCACA AATTTGGAAT 
GCGATTGTCA TTTCTATGTA TCGTGAACAT TTACTTTCCA TTGATCATGT ACAACGTCTT 
CTGTATCATC AAGTTCCAGC TGAAGAAGGA CGTAGGACTC TTAGAACCCC TACGTTTTTT 
GTCTCTCAGG ACGATAATAT TGTTCACACT ACTTTCTTCC CTGCAAATTC AGAGGCAGAG 
CGTCGTCTTT CTTTCTTTGC ACAATCTTTA GCTACTCCCA TTCCAGAACC AGTACCTGTT 
GACAACATGC CTACTTTCAC TGTATTGATT CCTCATTATG CTGAGAAAAT TCTTCTATCA 
TTGCGAGAAA TCATTAGAGA AGAGGACCAG TTGTCTCGTG TCACCCTTTT AGAATATTTG 
AAACAGCTTC ATCCTGTTGA ATGGGACTGT TTTGTGAAGG ATACTAAGAT TCTGGTAGAG 
GAAAATGCTC CATATGAAAA TGATTCTGTC TCTGAAAAGG AAGGTACATA TAAAAGTAAA 
GTTGATGATC TTCCTTTTTA CTGTATTGGT TTCAAATCTG CTATGCCCGA GTATACGTTA 
CGTACCCGTA TTTGGGCTTC CTTAAGAAGT CAAACGTTGT ATCGTACTAT CAGCGGTTTT 
ATGAACTATA GCAGAGCTAT CAAGCTTTTA TATCGTGTTG AAAACCCCGA AATCGTTCAA 
ATGTTTGGTG GTAACACAGA TCGTTTGGAA AGGGAGTTAG ATCGCATGGC TCGTCGAAAA 
TTCAAGTTGG TTGTCTCAAT GCAACGTTAT GCAAAGTTCA CAAAGGAAGA GTATGAAAAT 
GCCGAATTTC TTCTCAGGGC TTATCCTGAT TTGCAAATTG CTTACCTTGA CGAAGATCCT 
CCTGAAGAAG AAGGTGCGGA ACCTCAATTG TTTGCTGCTT TGATTGACGG TCACTCTGAA 
ATTATGGAAA ATGAACGTCG TCGTCCGAAA TATCGTATTC GTTTGTCTGG TAATCCTATT 
CTTGGTGATG GTAAATCTGA CAATCAAAAC ATGAGCTTAC CATTCTATCG TGGTGAATAT 
ATTCAACTTA TTGATGCTAA TCAAGATAAT TATCTTGAAG AGTGCCTTAA GATTCGAAGT 
GTTCTTGCTG AGTTTGAAGA AATGGAGACC GACAATGTTA ACCCCTATTC TGAAAGTGCT 
CGTGAAAGAA ATAAGCATCC TGTAGCTATT CTTGGTGCTC GTGAATACAT TTTCTCTGAG 
AACATTGGTA TATTGGGTGA CGTTGCAGCA GGCAAAGAAC AAACATTCGG TACTCTTTTC 
TCTCGTACAC TTGCGCAAAT TGGCGGTAAA CTTCATTATG GTCATCCAGA TTTTCTTAAC 
GGTATCTTTA TGACTACACG TGGCGGTGTT TCTAAAGCTC AAAAAGGTTT ACACGTCAAC 
GAAGATATTT ATGCTGGTAT GAATGCTATG CTTCGCGGTG GACGTATCAA GCATTGCGAG 
TATTTCCAAT GTGGTAAAGG TCGTGATTTA GGTTTTGGCT CAATTTTGAA TTTCAACACA 
AAAGTTGGTA CTGGTATGGG TGAACAAATG TTATCCCGTG AGTATTATTA CTTAGGTACG 
CAGCTTCAAC TTGACCGTTT CCTTTCTTTT TATTTTGCTC ACCCCGGTTT CCATCTTAAC 
AATATGTTTA TTATGCTTTC TGTTCAACTT TTCATGGTGG TCCTTATCAA CTTAGGAGCA 
ATTTATCACG TTGTCACTGT TTGTTATTAC AACGGTAATC AAAAGCTTAG TTACGACACT 
AGTATCGTTC CACGTGGTTG TTATCAATTG GGACCCGTTT TGTCTTGGCT GAAGAGATGT 
GTTATTTCAA TTTTCATTGT GTTTTGGATT TCGTTTATTC CCCTTACTGT GCATGAATTA 
ATTGAACGTG GTGTTTGGAG AGCTACAAAA AGATTCTTCA AGCAAATTGG TTCTTTCTCC 
CCCTTGTTTG AGGTTTTTAC CTGCCAAGTG TACTCTCAAG CTATTACCTC GGATCTTGCA 
TACGGCGGTG CTAGATATAT TGGTACTGGT CGTGGTTTTG CTACTGCTCG TTTACCTTTC 
TCCATCTTGT ATTCACGCTT TGCAGTCCCT TCAATTTACA TTGGAGCTAG ATTTCTAATG 
ATGTTGTTGT TTGGTACTAT GACTGTTTGG GTTGCGCACT TGATCTATTG GTGGGTGTCA 
ATCATGGCTC TTTGTGTTGC TCCATTTTTG TTCAATCCTC ATCAATTTGA TTGGAACGAT 
TTCTTTGTTG ATTATAGAGA GTTTATTCGC TGGCTTTCGA GAGGTAATTC TCGTTCTCAT 
GCCAATTCAT GGATCGGATA TTGTCGATTA ACCCGCACTA GAATAACTGG TTACAAACGT 
AGGGTACTAG GTCAACCAAG CGATAAAATT TCAATGGATA CTCCACGAGC AAAGTTTACG 
AATGTTTTCT TTAGCGATGT GTTAATCCCT GCCTTGTTAG CTGCTGGTGC CATTATCCCT 
TACTTCTTTA TCAATTCTCA GCCTGGAAAT CCCATGTTTA TCACTGATCC AAATAATCCA 
AGCCCATATG TTCATGACAC AAAGACAGGT ACTAATCCCA TTTTGCGACT TGTTATTATC 
TCTCTCATTC CTATTGCAGC AGGCTTTGGT ATGTCAGGTT TCTTTGGTGG TATGGCTTGT 
TGTTTGGGTC CAGCCTTTGG ATTGTGCTGT AAAAAGTTTC CATCTATTTT TGCTGCAATT 
GCCCACACGA TCCAAATTTT CATCTTCATT GCTATTTTTG AAGTCTGCTG GTTCCTTGAT 
GGATGGTCAT TACCCAAGAC TGTGTTGGCC TTCTGTGCTG TTACTGCAAT TCATCGTTTT 
ATCTTCAAAA TTTTAACACT ATTATGTTTA TCTCGTGAGG TCAAACAAGA TAGTGCTAAT 
ATTTCTTGGT GGAGTGGTAA ATGGTATGGC AAAGGATATG GCTATCATGC CTTTACTTTG 
CCTGCTCGTG AATTTGTCTG TAAAGCCATA GAACTTAATT TGTTTGCTAC TGATTTCTTC 
TTGGGTCACT TATTACTGTT TTTTATGCTT CCTGTGATTT GTATCCCATA TATTGATCGT 
TGGCATTCAG TTCTTCTTTT CTGGTTGCGC CCAAGTCGTC AAATTCGTCC TCCTATTTTC 
TCTACCAAGC AGAATAGACT GCGGAAACGT ATTGTTCGTC GTTATTCAGC CTTGTACTTT 
TCAATTTTGG TAATATTCCT TATTCTTATT ATTGTTCCAT TGGCAGCTGG TGCAGAAATC 
CGCCAAGGAT TAACTGCTTC TGAGGCTGTT GCCAAGGGTG CTGTTGGATG GAACCAAACT 
AACTCTTCAA TTGGTAGCGG TATCATTCAA CCAAGGGACA CGAATTATAC TGCTAATTAT 
TCTTTCTGGT ACGACAGATA CCATTTCGAA TTCAATACCA CTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588501.1
CDS	1561..7428
Translation	MSGNNENVSGITGHDAVSDDQYAYDSEVYDDQNAYQRQPADAYSNEEFLDQADYDSMYGEGYNGYDYPTGVTESYGDEYTPVDTASSGINQYSTEKGKFTRPSDEYESEYSDYNAQPSDANNFYNLRGDGRYNAYDPSSDSLANVYNSVPYGSSPYDFSNSSFVGNSGSGTPLDGDSGSFYADSANLTNREPYPAWTPENELPLTKEEIEDIFIDLTNKLGFQRDSMRNMYDFFMCLLDSRASRMTPDQALLTLHADYIGSDIANYKKWYFASQMDREDAVGLANVGIYGGKVTSIKEKGKFFSRNKKAPKVVKPPRKSRFKRKKKKEQPEEAEDEYIDVNTDDSLESAEYRWRSHMRSMTQFERAQQIALWLLLWGEANNVRFMPEVIAFLFKCAYDYIISPEAQNVTEPVPEGYYLDNIVSPLYQYMHDQQFEIINGKYVRRERPHDQLIGYDDINQLFWHAEGIARLIFEDGTRLIDIPASERFHRLPEVQWNRAFYKTYYESRSWFHLITNFNRIWVIHFGMFWYFTAFNSPTLYTKPFHQRDGPKPTGASQWAAVACTSVVSCIIMAAASLCEYLFVPRRFPGSKPIWKRLCIIVLIAIINLIPIVYIFGFSSKHQQRSGRRIAVGVVAFLMSIATYVYFSLVPLQSTFGKLSVKDSRKYLANKYFTSNFAPLKFDNQALSVIIWVCVFTCKFAESYFFLTLSIRDPIIVLSTMRPYLCSIYWAGSRLCFVQPRIILGIMYFTDLILFFLDTYLWYIIFNTIFSVLRSFVLGISILTPWRNIFSRMPQRIYGKILATNDMEIKYKPKILISQIWNAIVISMYREHLLSIDHVQRLLYHQVPAEEGRRTLRTPTFFVSQDDNIVHTTFFPANSEAERRLSFFAQSLATPIPEPVPVDNMPTFTVLIPHYAEKILLSLREIIREEDQLSRVTLLEYLKQLHPVEWDCFVKDTKILVEENAPYENDSVSEKEGTYKSKVDDLPFYCIGFKSAMPEYTLRTRIWASLRSQTLYRTISGFMNYSRAIKLLYRVENPEIVQMFGGNTDRLERELDRMARRKFKLVVSMQRYAKFTKEEYENAEFLLRAYPDLQIAYLDEDPPEEEGAEPQLFAALIDGHSEIMENERRRPKYRIRLSGNPILGDGKSDNQNMSLPFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEEMETDNVNPYSESARERNKHPVAILGAREYIFSENIGILGDVAAGKEQTFGTLFSRTLAQIGGKLHYGHPDFLNGIFMTTRGGVSKAQKGLHVNEDIYAGMNAMLRGGRIKHCEYFQCGKGRDLGFGSILNFNTKVGTGMGEQMLSREYYYLGTQLQLDRFLSFYFAHPGFHLNNMFIMLSVQLFMVVLINLGAIYHVVTVCYYNGNQKLSYDTSIVPRGCYQLGPVLSWLKRCVISIFIVFWISFIPLTVHELIERGVWRATKRFFKQIGSFSPLFEVFTCQVYSQAITSDLAYGGARYIGTGRGFATARLPFSILYSRFAVPSIYIGARFLMMLLFGTMTVWVAHLIYWWVSIMALCVAPFLFNPHQFDWNDFFVDYREFIRWLSRGNSRSHANSWIGYCRLTRTRITGYKRRVLGQPSDKISMDTPRAKFTNVFFSDVLIPALLAAGAIIPYFFINSQPGNPMFITDPNNPSPYVHDTKTGTNPILRLVIISLIPIAAGFGMSGFFGGMACCLGPAFGLCCKKFPSIFAAIAHTIQIFIFIAIFEVCWFLDGWSLPKTVLAFCAVTAIHRFIFKILTLLCLSREVKQDSANISWWSGKWYGKGYGYHAFTLPAREFVCKAIELNLFATDFFLGHLLLFFMLPVICIPYIDRWHSVLLFWLRPSRQIRPPIFSTKQNRLRKRIVRRYSALYFSILVIFLILIIVPLAAGAEIRQGLTASEAVAKGAVGWNQTNSSIGSGIIQPRDTNYTANYSFWYDRYHFEFNTTY

Target ORF information:

RefSeq Version	NM_001023491.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe 1,3-beta-glucan synthase subunit Bgs4 (bgs4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023491.2

ORF Insert Sequence:

ATGTCTGGAA ATAATGAAAA TGTTTCAGGT ATTACTGGTC ATGATGCTGT CTCGGATGAT 
CAGTACGCTT ACGATTCAGA GGTTTATGAT GACCAAAATG CATACCAACG CCAGCCTGCT 
GATGCGTATT CCAATGAGGA GTTTTTGGAT CAGGCTGACT ACGATTCTAT GTATGGAGAG 
GGTTACAATG GGTATGATTA CCCTACTGGT GTAACTGAAA GTTATGGAGA TGAATACACT 
CCTGTCGACA CCGCCTCTTC AGGTATCAAT CAATATTCCA CTGAAAAAGG AAAATTTACT 
CGTCCTTCAG ATGAATACGA GTCTGAGTAT TCAGATTACA ATGCTCAGCC CTCAGATGCC 
AACAATTTTT ACAATTTGAG AGGCGATGGT AGATACAACG CTTATGATCC TAGTTCCGAT 
TCGTTGGCTA ACGTCTACAA CAGTGTGCCT TACGGTAGCA GTCCGTATGA TTTTAGCAAC 
TCTTCTTTTG TTGGTAATTC GGGCTCAGGT ACCCCATTGG ATGGCGATAG TGGTTCATTC 
TATGCCGATA GCGCTAATCT CACAAATCGT GAGCCTTATC CTGCCTGGAC TCCTGAAAAC 
GAACTCCCTT TAACTAAGGA AGAGATTGAA GACATATTCA TTGATCTAAC CAATAAATTG 
GGTTTCCAAC GCGATTCCAT GCGTAATATG TATGATTTTT TTATGTGTCT TTTAGATTCT 
CGTGCTTCTC GCATGACTCC AGATCAAGCA CTGCTTACGC TTCATGCCGA TTATATTGGT 
AGTGATATTG CTAACTATAA AAAATGGTAT TTTGCTTCTC AGATGGATCG GGAAGATGCA 
GTCGGTCTTG CTAATGTTGG CATTTATGGT GGTAAAGTTA CTTCTATCAA AGAGAAAGGA 
AAGTTCTTTT CTCGAAACAA AAAAGCCCCT AAAGTTGTTA AGCCTCCAAG GAAGTCTCGC 
TTCAAGCGCA AGAAGAAAAA GGAGCAACCC GAGGAGGCGG AAGACGAATA CATTGATGTT 
AATACCGATG ATTCTTTAGA ATCCGCCGAA TATCGTTGGA GATCTCACAT GCGCTCAATG 
ACTCAATTCG AGCGTGCTCA ACAAATTGCA TTGTGGCTTT TGTTGTGGGG TGAGGCAAAC 
AATGTTCGTT TCATGCCTGA GGTAATAGCC TTCCTTTTCA AATGTGCTTA CGATTATATT 
ATTTCACCTG AGGCACAAAA CGTCACTGAA CCAGTTCCTG AAGGATATTA CTTGGATAAT 
ATTGTTTCCC CACTCTACCA ATATATGCAC GATCAGCAGT TTGAAATTAT CAATGGGAAA 
TATGTACGTC GGGAACGTCC ACATGACCAG TTAATTGGAT ACGATGATAT TAACCAACTT 
TTCTGGCATG CTGAGGGTAT AGCACGTCTT ATTTTTGAAG ACGGCACTCG TCTTATTGAT 
ATTCCTGCTT CTGAGCGTTT TCATAGGCTT CCAGAAGTTC AATGGAATCG TGCTTTTTAT 
AAAACTTATT ATGAATCCCG TTCTTGGTTT CATCTTATTA CCAATTTCAA TCGTATTTGG 
GTCATCCACT TTGGTATGTT TTGGTATTTT ACTGCTTTCA ACTCTCCTAC TCTTTACACA 
AAACCTTTCC ATCAGCGCGA TGGTCCCAAG CCAACAGGTG CTTCCCAATG GGCTGCTGTT 
GCTTGTACCT CTGTTGTTTC TTGTATTATC ATGGCTGCTG CTTCTTTGTG TGAATATCTT 
TTTGTCCCCC GTCGTTTCCC TGGATCAAAA CCCATTTGGA AGCGTTTATG TATCATTGTT 
TTAATCGCTA TCATTAACTT GATTCCTATC GTCTATATCT TCGGTTTCTC AAGTAAGCAT 
CAACAGCGTA GTGGTCGACG TATCGCTGTT GGTGTAGTTG CATTTTTGAT GTCTATTGCT 
ACATACGTCT ACTTTTCTCT TGTACCTCTA CAAAGTACAT TTGGAAAATT GTCCGTGAAA 
GATTCTCGTA AATACTTGGC AAACAAGTAT TTCACTTCAA ATTTTGCACC ATTAAAATTT 
GATAACCAAG CACTCTCTGT TATTATTTGG GTCTGTGTTT TCACATGCAA ATTTGCTGAA 
TCTTATTTCT TCCTTACCTT GTCTATCAGA GATCCTATTA TTGTTTTGAG TACCATGCGA 
CCTTATCTTT GCTCTATTTA TTGGGCGGGT AGCAGATTAT GTTTTGTCCA ACCAAGGATC 
ATTTTGGGTA TCATGTATTT TACTGACTTG ATTCTCTTCT TTTTGGATAC GTACCTTTGG 
TATATTATTT TCAATACTAT CTTCTCTGTT TTAAGATCAT TCGTCTTAGG TATTTCGATC 
CTCACACCAT GGAGAAATAT CTTTAGCCGT ATGCCCCAAA GAATTTATGG TAAAATTTTG 
GCCACGAATG ACATGGAAAT TAAGTACAAA CCTAAAATTT TAATTTCACA AATTTGGAAT 
GCGATTGTCA TTTCTATGTA TCGTGAACAT TTACTTTCCA TTGATCATGT ACAACGTCTT 
CTGTATCATC AAGTTCCAGC TGAAGAAGGA CGTAGGACTC TTAGAACCCC TACGTTTTTT 
GTCTCTCAGG ACGATAATAT TGTTCACACT ACTTTCTTCC CTGCAAATTC AGAGGCAGAG 
CGTCGTCTTT CTTTCTTTGC ACAATCTTTA GCTACTCCCA TTCCAGAACC AGTACCTGTT 
GACAACATGC CTACTTTCAC TGTATTGATT CCTCATTATG CTGAGAAAAT TCTTCTATCA 
TTGCGAGAAA TCATTAGAGA AGAGGACCAG TTGTCTCGTG TCACCCTTTT AGAATATTTG 
AAACAGCTTC ATCCTGTTGA ATGGGACTGT TTTGTGAAGG ATACTAAGAT TCTGGTAGAG 
GAAAATGCTC CATATGAAAA TGATTCTGTC TCTGAAAAGG AAGGTACATA TAAAAGTAAA 
GTTGATGATC TTCCTTTTTA CTGTATTGGT TTCAAATCTG CTATGCCCGA GTATACGTTA 
CGTACCCGTA TTTGGGCTTC CTTAAGAAGT CAAACGTTGT ATCGTACTAT CAGCGGTTTT 
ATGAACTATA GCAGAGCTAT CAAGCTTTTA TATCGTGTTG AAAACCCCGA AATCGTTCAA 
ATGTTTGGTG GTAACACAGA TCGTTTGGAA AGGGAGTTAG ATCGCATGGC TCGTCGAAAA 
TTCAAGTTGG TTGTCTCAAT GCAACGTTAT GCAAAGTTCA CAAAGGAAGA GTATGAAAAT 
GCCGAATTTC TTCTCAGGGC TTATCCTGAT TTGCAAATTG CTTACCTTGA CGAAGATCCT 
CCTGAAGAAG AAGGTGCGGA ACCTCAATTG TTTGCTGCTT TGATTGACGG TCACTCTGAA 
ATTATGGAAA ATGAACGTCG TCGTCCGAAA TATCGTATTC GTTTGTCTGG TAATCCTATT 
CTTGGTGATG GTAAATCTGA CAATCAAAAC ATGAGCTTAC CATTCTATCG TGGTGAATAT 
ATTCAACTTA TTGATGCTAA TCAAGATAAT TATCTTGAAG AGTGCCTTAA GATTCGAAGT 
GTTCTTGCTG AGTTTGAAGA AATGGAGACC GACAATGTTA ACCCCTATTC TGAAAGTGCT 
CGTGAAAGAA ATAAGCATCC TGTAGCTATT CTTGGTGCTC GTGAATACAT TTTCTCTGAG 
AACATTGGTA TATTGGGTGA CGTTGCAGCA GGCAAAGAAC AAACATTCGG TACTCTTTTC 
TCTCGTACAC TTGCGCAAAT TGGCGGTAAA CTTCATTATG GTCATCCAGA TTTTCTTAAC 
GGTATCTTTA TGACTACACG TGGCGGTGTT TCTAAAGCTC AAAAAGGTTT ACACGTCAAC 
GAAGATATTT ATGCTGGTAT GAATGCTATG CTTCGCGGTG GACGTATCAA GCATTGCGAG 
TATTTCCAAT GTGGTAAAGG TCGTGATTTA GGTTTTGGCT CAATTTTGAA TTTCAACACA 
AAAGTTGGTA CTGGTATGGG TGAACAAATG TTATCCCGTG AGTATTATTA CTTAGGTACG 
CAGCTTCAAC TTGACCGTTT CCTTTCTTTT TATTTTGCTC ACCCCGGTTT CCATCTTAAC 
AATATGTTTA TTATGCTTTC TGTTCAACTT TTCATGGTGG TCCTTATCAA CTTAGGAGCA 
ATTTATCACG TTGTCACTGT TTGTTATTAC AACGGTAATC AAAAGCTTAG TTACGACACT 
AGTATCGTTC CACGTGGTTG TTATCAATTG GGACCCGTTT TGTCTTGGCT GAAGAGATGT 
GTTATTTCAA TTTTCATTGT GTTTTGGATT TCGTTTATTC CCCTTACTGT GCATGAATTA 
ATTGAACGTG GTGTTTGGAG AGCTACAAAA AGATTCTTCA AGCAAATTGG TTCTTTCTCC 
CCCTTGTTTG AGGTTTTTAC CTGCCAAGTG TACTCTCAAG CTATTACCTC GGATCTTGCA 
TACGGCGGTG CTAGATATAT TGGTACTGGT CGTGGTTTTG CTACTGCTCG TTTACCTTTC 
TCCATCTTGT ATTCACGCTT TGCAGTCCCT TCAATTTACA TTGGAGCTAG ATTTCTAATG 
ATGTTGTTGT TTGGTACTAT GACTGTTTGG GTTGCGCACT TGATCTATTG GTGGGTGTCA 
ATCATGGCTC TTTGTGTTGC TCCATTTTTG TTCAATCCTC ATCAATTTGA TTGGAACGAT 
TTCTTTGTTG ATTATAGAGA GTTTATTCGC TGGCTTTCGA GAGGTAATTC TCGTTCTCAT 
GCCAATTCAT GGATCGGATA TTGTCGATTA ACCCGCACTA GAATAACTGG TTACAAACGT 
AGGGTACTAG GTCAACCAAG CGATAAAATT TCAATGGATA CTCCACGAGC AAAGTTTACG 
AATGTTTTCT TTAGCGATGT GTTAATCCCT GCCTTGTTAG CTGCTGGTGC CATTATCCCT 
TACTTCTTTA TCAATTCTCA GCCTGGAAAT CCCATGTTTA TCACTGATCC AAATAATCCA 
AGCCCATATG TTCATGACAC AAAGACAGGT ACTAATCCCA TTTTGCGACT TGTTATTATC 
TCTCTCATTC CTATTGCAGC AGGCTTTGGT ATGTCAGGTT TCTTTGGTGG TATGGCTTGT 
TGTTTGGGTC CAGCCTTTGG ATTGTGCTGT AAAAAGTTTC CATCTATTTT TGCTGCAATT 
GCCCACACGA TCCAAATTTT CATCTTCATT GCTATTTTTG AAGTCTGCTG GTTCCTTGAT 
GGATGGTCAT TACCCAAGAC TGTGTTGGCC TTCTGTGCTG TTACTGCAAT TCATCGTTTT 
ATCTTCAAAA TTTTAACACT ATTATGTTTA TCTCGTGAGG TCAAACAAGA TAGTGCTAAT 
ATTTCTTGGT GGAGTGGTAA ATGGTATGGC AAAGGATATG GCTATCATGC CTTTACTTTG 
CCTGCTCGTG AATTTGTCTG TAAAGCCATA GAACTTAATT TGTTTGCTAC TGATTTCTTC 
TTGGGTCACT TATTACTGTT TTTTATGCTT CCTGTGATTT GTATCCCATA TATTGATCGT 
TGGCATTCAG TTCTTCTTTT CTGGTTGCGC CCAAGTCGTC AAATTCGTCC TCCTATTTTC 
TCTACCAAGC AGAATAGACT GCGGAAACGT ATTGTTCGTC GTTATTCAGC CTTGTACTTT 
TCAATTTTGG TAATATTCCT TATTCTTATT ATTGTTCCAT TGGCAGCTGG TGCAGAAATC 
CGCCAAGGAT TAACTGCTTC TGAGGCTGTT GCCAAGGGTG CTGTTGGATG GAACCAAACT 
AACTCTTCAA TTGGTAGCGG TATCATTCAA CCAAGGGACA CGAATTATAC TGCTAATTAT 
TCTTTCTGGT ACGACAGATA CCATTTCGAA TTCAATACCA CTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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bgs4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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