ORF » Species Summary » Neurospora crassa OR74A » NCU01782 cDNA ORF clone

NCU01782 cDNA ORF clone, Neurospora crassa OR74A

The following NCU01782 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU01782 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf10303 XM_952112.2
Latest version!		 Neurospora crassa OR74A Ras guanyl-nucleotide exchange factor RasGEF (NCU01782), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NCU01782 ( XM_952112.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957205.2 Neurospora crassa OR74A Ras guanyl-nucleotide exchange factor RasGEF (NCU01782), mRNA.
    Price & Availability↑
    CloneID ONf10303
    Clone ID Related Accession (Same CDS sequence) XM_952112.2
    Accession Version XM_952112.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product Ras guanyl-nucleotide exchange factor RasGEF
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_952112.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCCATGT CGGAACCACC ACTGTCGGAC GCACTGGCAG GCCCCCCGCT GGAGCAGTCG 
    CCGTCGCAAT TACAGCCTAC ACCACTGACC GAGTCGCGCC GGCTGATCAG TCAAGTCAAG 
    CAAATGCTGG GTCTCACGGG GTCGGGTCAC GCTGAAGCTG ACGATCCCCA ATTGCCCGAG 
    CTCCTCCCCA AGTGGAAGCT CCCCAACCTG GACCGGCTGG ACCTGGATCT GGGCCTGGAG 
    CTGCCGGCCG ATCTGTGTGC GCCCATCGAG TTTGCATTGC CGCTTCAGTG TACACCTACT 
    ACAAAACTAG GCACCACTCA TCATCCACTG CTAGCAAGCC CGGCGGGTGC TGCCGATCCG 
    CATCTCCTCC ACCAGGACCA CCAAAACACC AACAAGACCA CCAATCTCGA CGACGCCACG 
    ACCGCTACTT CCGCTTCTGC CGCTGCCATC TCGTCAACAA CACAGCCGAC CTCAGCAGCA 
    ACGCCATCGC TCCTCGTCTC CCAGGGCGCC CTTTTGCTGC CAGCCGACGA CGACCATCCC 
    GGCCCCGGCC CCTCCTCCAA TTACCGCGGC TCCTCTGCTT CTGCCGTCCT CGACGATCCC 
    TTCTTCTGCC GACCTGTGGG CGCGCCCAAT ACCCATCCCA CCGACACTGC CACCACGCCT 
    GATTGCCATC CTAGTCTGAT TCTCAGTCAG ACACCCAGTC CGGGTCCGGG TCCTGTTCCC 
    AGTCCCCAGT CTGAGTCGAC TTATAGCTAT AATCGCAATG ATCCGCACCG ACCGTGGCCT 
    CCGCCTCGCA AAGAGTCTCT GAGAGTCGAC AGCCCAACGC CGTGGTTCGA TCAATCGCGA 
    TCGAGACGTG CCATGGCATC CATACACATT GCCGTTATTG GCGCCTACGG CGCGGGCAAA 
    TCCTCCTTTG TCCAGCGCGC GTTCCGTTTG CAACGACCGC CCGAGCTAAA CATCTCGACC 
    TTGCGCATGG AAATCCATGG CACCAGATAC CTCGTCACCC TCGTAGAACT TGATCTTGAC 
    GGCTTCAAAG ACCTTGACCC CGGCCAGTCC ATACAATGGC CCAAGCAAAT AGACGGCCAC 
    ATGTTCCCCC ACATGCCCGA CGGCGCCCTG ATTCTGTACG ACGTCACCAA CACGGACAGC 
    ATCAACGATC TCTCCCCAAC CATGACTTCC CTGGCCAACT CCTCCATACC AACCATACTC 
    GTCGCGACGA AATGTGATAC CCCTGCGAGC ATACGCCAGG TGGATATGAA CGCCGTAGCA 
    CACAGCTTTC CCTCGTGTGT TGCCCATCTG AGGATCTCGT CCGATATTCC CGGCGGCGCG 
    CGTGAAGCCC TCTTGACCAT GATCGACGCC ATCCTGGCCA ACAGAAGAGA AGCGCAAGAG 
    AGACCGAAAA GGTCGGTTTC GAGACGAAGA GCTGCTTCGG CCGCTAATCT GGATGCTACC 
    CCCGAGGTCA ACGGTCGTCC CATCAGCCAG CACAGCAAAC ACAGCAGAGC AAGCTCGGAC 
    TTCTCGCTCT TGAAGGGCTT TGCGCCTCCT AGCGGCGACG GTCCTCACCA GAGATCCCAA 
    CTTTCGAGGT CGCCGAGAAT GGAATATCTA CATGCACAAT ACGGCGCTTC CAACCCGCAA 
    TCAACAGACA TTCCTGAGGA CGGTCTTCCA CACGCATTGT CAGGCACGCT ACGTACACCA 
    GGGATACGAT TGGAGAAAGG GTCGGAGACC TTCCTGGATT TTGAAGAGTT GGATCTGGAA 
    AGCCACCGCT TGTCGGACGA TGTAGCCACA ATGGCTCGCA ATCAGGATTA TCTGGAAGAC 
    GAAGCCCCTA AACTTGCCGG CGTCACCTTC GATGAGCTTA TCGACAGGAT TATAGCTCCA 
    AAGATGACAA GGGCGGACAA CAACTTTGCC GACATTTTTC TGTGCTTGTA TAGAAAGTTC 
    GCTACGCCAT GCGAGCTTAT GAGCGCTCTC CTTTCGCGGC TGGACCGTTT AAGGGACGGC 
    ACGGACATGC CGTCTTTGCT GAGCATCGAA AGCCAGATGC GCGTCATTGA GGTTGTTGCG 
    AAATGGCTGT CCCTTTACCC CGGAGACTTT GCCCGAACAG CAACGAGGAG GAGATTGGAA 
    GGACTAATAA GCGACTTGGC AACGAACGAG GTCTTTTCCG CAGCAGCCCG GCAGATGAAG 
    GGGAACCTGG AACAAAAGGT GGTGGACGAC GATGATACCT GGTGGGCAAA AGCGGACCCG 
    GTCGACGACG AAGATGACGA GGAGGGTGGC AGGGACGTAG AGCGGAAACT CGCCGAGTCT 
    GTGGCTTCAG TGCAGATCGA CGAGTCGGGC GGGTATGATC CTCGACGTCC ATCACACAGT 
    TCAGAGCTCT CGGGGGCTGA TCCGACTGCG AAATTCACCT ACCATTCCAT CGAAGACTAC 
    GAGCGCGAAG CCGCGGCCTT GGTACCCTCT CCCGTCTTAC CGCCGAACAA GATCCGCTAT 
    CACAGCTTCA TGGACATCGA TCCCGACATA ATCGCCGACG AGATTACGCG CATTGATTGG 
    ATCATGTTCA GCTCCATCCG CATCCGCGAC ATGGTGCGCC ACGTTAGTCT GCCCGCCGAC 
    CAGAAGGAGA AATGCCGCAG TCTCAAGAAC GTCAACCGCA TGGTCTCCCA CTTCAACCAC 
    ATAGCCCAAT GGGTGTCCAA CATGATCCTC ATTCGTGACA AAGCCAAACA TCGCGCACCC 
    TGCCTAGAAC GGTTCATGGT CATCGCCCAG CGCTTGCGCA CGCTGAACAA CTACAACGGC 
    CTGGCCGCCG TATTGGCCGG TATCAACGGC ACCGCCATCC ACCGACTCGC GCAGACGCGA 
    AATTTGGTTT CGCCTGACGT ACAGAAGCGG TTCGCTCGGC TGGTCCTGCT CATGGGCACG 
    CAAAAGAGCC ACTTTGCTTA CCGGCTGGCC TGGGAGAACA CGCCGCTACC GAGGATTCCG 
    TTCATCCCCC TGCACAGACG TGATCTTGTC TCGGCAGAGG AAGGAAGCAG GACATTCGTG 
    GGGCCGAATA ATGATCGGAT TAATTGGAAG AAATACGAGG TGCTCGGGGA GGTGTTGCTG 
    CCCATAATGA GGAGTCAGGG ACAGCCGTAT CCGAACTTGA CCAAGCACGA GCCTAGTAGG 
    GAGTTGATAC TTGATATGAA GATGCCGACG GACGAAGAGG ATATCTACCA ACGCAGTGTA 
    CAAGTAGAGC CGTCAACAGG TGGCGAGTCA ACTACCAGGA AGAAATTCGC TTGGCTCGTA 
    AACAAATCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_957205.2
    CDS658..3909
    Translation

    Target ORF information:

    RefSeq Version XM_952112.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A Ras guanyl-nucleotide exchange factor RasGEF (NCU01782), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_952112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCCATGT CGGAACCACC ACTGTCGGAC GCACTGGCAG GCCCCCCGCT GGAGCAGTCG 
    CCGTCGCAAT TACAGCCTAC ACCACTGACC GAGTCGCGCC GGCTGATCAG TCAAGTCAAG 
    CAAATGCTGG GTCTCACGGG GTCGGGTCAC GCTGAAGCTG ACGATCCCCA ATTGCCCGAG 
    CTCCTCCCCA AGTGGAAGCT CCCCAACCTG GACCGGCTGG ACCTGGATCT GGGCCTGGAG 
    CTGCCGGCCG ATCTGTGTGC GCCCATCGAG TTTGCATTGC CGCTTCAGTG TACACCTACT 
    ACAAAACTAG GCACCACTCA TCATCCACTG CTAGCAAGCC CGGCGGGTGC TGCCGATCCG 
    CATCTCCTCC ACCAGGACCA CCAAAACACC AACAAGACCA CCAATCTCGA CGACGCCACG 
    ACCGCTACTT CCGCTTCTGC CGCTGCCATC TCGTCAACAA CACAGCCGAC CTCAGCAGCA 
    ACGCCATCGC TCCTCGTCTC CCAGGGCGCC CTTTTGCTGC CAGCCGACGA CGACCATCCC 
    GGCCCCGGCC CCTCCTCCAA TTACCGCGGC TCCTCTGCTT CTGCCGTCCT CGACGATCCC 
    TTCTTCTGCC GACCTGTGGG CGCGCCCAAT ACCCATCCCA CCGACACTGC CACCACGCCT 
    GATTGCCATC CTAGTCTGAT TCTCAGTCAG ACACCCAGTC CGGGTCCGGG TCCTGTTCCC 
    AGTCCCCAGT CTGAGTCGAC TTATAGCTAT AATCGCAATG ATCCGCACCG ACCGTGGCCT 
    CCGCCTCGCA AAGAGTCTCT GAGAGTCGAC AGCCCAACGC CGTGGTTCGA TCAATCGCGA 
    TCGAGACGTG CCATGGCATC CATACACATT GCCGTTATTG GCGCCTACGG CGCGGGCAAA 
    TCCTCCTTTG TCCAGCGCGC GTTCCGTTTG CAACGACCGC CCGAGCTAAA CATCTCGACC 
    TTGCGCATGG AAATCCATGG CACCAGATAC CTCGTCACCC TCGTAGAACT TGATCTTGAC 
    GGCTTCAAAG ACCTTGACCC CGGCCAGTCC ATACAATGGC CCAAGCAAAT AGACGGCCAC 
    ATGTTCCCCC ACATGCCCGA CGGCGCCCTG ATTCTGTACG ACGTCACCAA CACGGACAGC 
    ATCAACGATC TCTCCCCAAC CATGACTTCC CTGGCCAACT CCTCCATACC AACCATACTC 
    GTCGCGACGA AATGTGATAC CCCTGCGAGC ATACGCCAGG TGGATATGAA CGCCGTAGCA 
    CACAGCTTTC CCTCGTGTGT TGCCCATCTG AGGATCTCGT CCGATATTCC CGGCGGCGCG 
    CGTGAAGCCC TCTTGACCAT GATCGACGCC ATCCTGGCCA ACAGAAGAGA AGCGCAAGAG 
    AGACCGAAAA GGTCGGTTTC GAGACGAAGA GCTGCTTCGG CCGCTAATCT GGATGCTACC 
    CCCGAGGTCA ACGGTCGTCC CATCAGCCAG CACAGCAAAC ACAGCAGAGC AAGCTCGGAC 
    TTCTCGCTCT TGAAGGGCTT TGCGCCTCCT AGCGGCGACG GTCCTCACCA GAGATCCCAA 
    CTTTCGAGGT CGCCGAGAAT GGAATATCTA CATGCACAAT ACGGCGCTTC CAACCCGCAA 
    TCAACAGACA TTCCTGAGGA CGGTCTTCCA CACGCATTGT CAGGCACGCT ACGTACACCA 
    GGGATACGAT TGGAGAAAGG GTCGGAGACC TTCCTGGATT TTGAAGAGTT GGATCTGGAA 
    AGCCACCGCT TGTCGGACGA TGTAGCCACA ATGGCTCGCA ATCAGGATTA TCTGGAAGAC 
    GAAGCCCCTA AACTTGCCGG CGTCACCTTC GATGAGCTTA TCGACAGGAT TATAGCTCCA 
    AAGATGACAA GGGCGGACAA CAACTTTGCC GACATTTTTC TGTGCTTGTA TAGAAAGTTC 
    GCTACGCCAT GCGAGCTTAT GAGCGCTCTC CTTTCGCGGC TGGACCGTTT AAGGGACGGC 
    ACGGACATGC CGTCTTTGCT GAGCATCGAA AGCCAGATGC GCGTCATTGA GGTTGTTGCG 
    AAATGGCTGT CCCTTTACCC CGGAGACTTT GCCCGAACAG CAACGAGGAG GAGATTGGAA 
    GGACTAATAA GCGACTTGGC AACGAACGAG GTCTTTTCCG CAGCAGCCCG GCAGATGAAG 
    GGGAACCTGG AACAAAAGGT GGTGGACGAC GATGATACCT GGTGGGCAAA AGCGGACCCG 
    GTCGACGACG AAGATGACGA GGAGGGTGGC AGGGACGTAG AGCGGAAACT CGCCGAGTCT 
    GTGGCTTCAG TGCAGATCGA CGAGTCGGGC GGGTATGATC CTCGACGTCC ATCACACAGT 
    TCAGAGCTCT CGGGGGCTGA TCCGACTGCG AAATTCACCT ACCATTCCAT CGAAGACTAC 
    GAGCGCGAAG CCGCGGCCTT GGTACCCTCT CCCGTCTTAC CGCCGAACAA GATCCGCTAT 
    CACAGCTTCA TGGACATCGA TCCCGACATA ATCGCCGACG AGATTACGCG CATTGATTGG 
    ATCATGTTCA GCTCCATCCG CATCCGCGAC ATGGTGCGCC ACGTTAGTCT GCCCGCCGAC 
    CAGAAGGAGA AATGCCGCAG TCTCAAGAAC GTCAACCGCA TGGTCTCCCA CTTCAACCAC 
    ATAGCCCAAT GGGTGTCCAA CATGATCCTC ATTCGTGACA AAGCCAAACA TCGCGCACCC 
    TGCCTAGAAC GGTTCATGGT CATCGCCCAG CGCTTGCGCA CGCTGAACAA CTACAACGGC 
    CTGGCCGCCG TATTGGCCGG TATCAACGGC ACCGCCATCC ACCGACTCGC GCAGACGCGA 
    AATTTGGTTT CGCCTGACGT ACAGAAGCGG TTCGCTCGGC TGGTCCTGCT CATGGGCACG 
    CAAAAGAGCC ACTTTGCTTA CCGGCTGGCC TGGGAGAACA CGCCGCTACC GAGGATTCCG 
    TTCATCCCCC TGCACAGACG TGATCTTGTC TCGGCAGAGG AAGGAAGCAG GACATTCGTG 
    GGGCCGAATA ATGATCGGAT TAATTGGAAG AAATACGAGG TGCTCGGGGA GGTGTTGCTG 
    CCCATAATGA GGAGTCAGGG ACAGCCGTAT CCGAACTTGA CCAAGCACGA GCCTAGTAGG 
    GAGTTGATAC TTGATATGAA GATGCCGACG GACGAAGAGG ATATCTACCA ACGCAGTGTA 
    CAAGTAGAGC CGTCAACAGG TGGCGAGTCA ACTACCAGGA AGAAATTCGC TTGGCTCGTA 
    AACAAATCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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