NCU05270 cDNA ORF clone, Neurospora crassa OR74A

The following NCU05270 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05270 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf03411 XM_956634.2
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Neurospora crassa OR74A mitochondrial translation initiation factor IF-2 (NCU05270), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf03411
    Clone ID Related Accession (Same CDS sequence) XM_956634.2
    Accession Version XM_956634.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product mitochondrial translation initiation factor IF-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_956634.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCCCA AGAAGAAGGG AAACAAGAAG GCCAACGATG ACTGGGAGGC CGAGCTGGGC 
    GAGTCCATCG CCCCCGTCAA CAACAACGGC GCCGATGCTG CTGCCGCCTC TCCCGCTGCC
    GCCGACGACG AGGCCGCTTC CGGTGGTGGC GGTCTGATGG AGAGAATGCG CAAGGCCAAG
    GAGAAGCGCA AGAAGAAGGG TCTTGCCGAC GACTGGGAGG CTGAGCTGGG CGAAGACGCT
    CCCGGCGCCG AGAAGCCCGC TGCCGCCGAG CCCGTCCCCG ATCTGTCGGC CAAGGTCGCC
    GAGGAGGCCA ACCTCGACGA CGAGTTCGCT CTCCCCGAGA AGAAGGGCAA GGGTGGCAAG
    GGCAAGCAGC AGCAGCAGCA ACAGAAGGCT GCTCCCGCCG CCGCCGCCAA GAAGGACGAG
    GATGCTGCCG ATGAGGATGG CCCTAGGGTG TTGACCAAGG CCGAGAAGGA GAAGCTCAAG
    AAGGAGCGTG AGAAGCAGCG CAAGAAGGAG CAGGCTGCTG CTAAGAAGAA GGGCACCACA
    CCCGCCGCCG CCGCCAAGGC CGAGCCCGCA AAGGAGGAGA AGAAGGCCGA AACCCCCAGC
    CCCGTCTCGA CCCCCGCTTC CACTCCCGCC CCCGAAGCCG CCGCCGGTGG CAAGAAGAAG
    AAGCTCCCCG CCCACCTGCT CGCCCTCCAG AAGCAGCAGG AGGAGCTGCG CCGTCGCCAA
    GAGGAGGAGG CTAGGCTCCT CGCCGAGGAG AAGGCCAGGA TAGAGGAGGA GGAGCGCGCC
    GCTGCCGAGG AGGCCAAGCG CAAGGAGGAG GAGCGCGCCC GCAAGAAGGA GAAGGAGAAG
    CAGAGGATCG AGCAGCTCAA GAAGGAGGGC AAGTTCATGA CCAAGGCCCA GAAGGAGGAG
    AAGGCCCGCA ACGAGAGGAA GCTGCAGCAG ATGTTGGCGG CCGGTATCAA GGTCAACGCG
    CTCGAGGGTG AAGAGTCCAA GAAGAAGCCT GTCTACGACG ACAAGAAGAA GAGGGCTGCT
    AACAAGAAGA AGGACGAGGA AAAGGCTCTC GCCGAGGCCG CTGAGCGCGC AAGACTCGCC
    GCTGAGGCTC TGGTCAAGGA GCAAGAGGAG AAGGCCCGCA TCGAGCGCGA GAAGGCCGAG
    AAGGAGGCTG CTGCCAAGGC TGAGGCCGCC AAGGCCGCCG CTGAGGACAG CGTCGACGAG
    GACTGGGAGG CCGCTGTTGC CTCTGACAAG GAGGATGTCA AGGACAGCTG GGACGCCGAC
    TCCGACGAGG AGGAGGAGAC CAAGAAGGAA GAGACCAAGA AGGAGGCTTC TAAGCCCGCC
    GCGGCCAACG GCAAGACTCA GGCCAAGAAG GAGGAGTCCG AGTCCGAGTC CGAGTCTGAG
    GAGGAATCTG AAGAGGAATC TTCTGAAGAC GAGGAGGCTA CCGCCAGGGA ACTGGAGGAG
    GCCCGGAGGA AGAAGGAGGC TGCCGAGCGC CGCGAGAAGG CTCACCAGGC CGCTCTGGCT
    GCCAGGTCTG CGGACAACCT CCGCTCGCCT ATTTGCTGTA TTCTTGGTCA CGTCGATACT
    GGTAAGACGA AGCTGCTCGA CAAGATTCGT CAGACCAACG TCCAGGAGGG TGAAGCCGGT
    GGTATCACTC AGCAGATTGG TGCCACTTAC TTCCCCGTTG AGGCCATCAA GCAGAAGACG
    CACGTCGTCA ACAGGGACGG CAAGTTCGAG TTCAAGGTTC CCGGTCTTCT TATCATCGAT
    ACCCCCGGTC ACGAGTCTTT CTCCAACTTG CGTTCGCGTG GTTCTTCGCT CTGCAACATC
    GCCATCTTGG TTGTCGATAT CATGCACGGT CTCGAACCCC AGACTCTCGA GTCCATGAGA
    CTGCTCCGTG AGAGGAAGAC GCCCTTCATC GTCGCTCTCA ACAAGATCGA TCGTCTTTAC
    GGCTGGAAGA AGATCGAGAA CAACGGCTTC CAGGAGTCGT TGGCCCTCCA GAACAAGGCT
    GTCCAGAACG AGTTCAAGAA CCGTCTCGAG CAGACCAAGC TCGCCTTCGC CGAGCAGGGT
    TTCAACTCGG AGCTCTTCTA CCAGAACAAG TCCATGGCCA AATACGTCTC CTTGGTCCCC
    ACCTCTGCCC ACACCGGCGA GGGTATCCCC GACATGTTGA AGCTCATTGT CCAGCTTACC
    CAGGAGCGCA TGGTTGGCTC TCTCATGTAC CTCTCCGAGG TCCAGGCTAC CGTTCTCGAA
    GTCAAGGCCA TCGAGGGTTT CGGTATGACC ATCGATGTCA TTCTCACCAA CGGTATCCTC
    AGGGAAGGTG ACCGCATTAT CCTATGTGGT ACAGAAGGCG TCATCAAAAC CAATATCAGA
    GCCCTGCTCA CGCCCGCGCC GCTCAGGGAA CTGCGCCTCA AGTCGCAGTA CGTTCACAAT
    AAGGAAGTCA AGGCCGCTTT GGGTGTCAAG ATCTCTGCCC CCGGCCTCGA GGGTGCCATT
    GCCGGTTCCC GTCTGCTTGT CGTCGGCCCC GACGACGACG AGGACGATCT CGAGGACGAG
    GTCGAGGCTG ATCTCGCCTC GCTCTTCAGC CGTGTCGAAA AGTCCGGCCG CGGTGTCTCC
    GTCCAGGCCT CCACCCTTGG TTCGCTCGAG GCCTTGCTCG ACTTCCTCAA GGAGTGCAAG
    ATCCCCGTCG CCAACGTCGG TATCGGTCCC GTCTACAAGC GTGATGTCAT GCAGTGCGGT
    ATCATGTTGG AGAAGGCTCC CGATTATGCC GTCATGCTCT GCTTCGATGT TAAGGTCGAC
    AAGGAAGCGC AGGAGTATGC CGATCAGCAG GGCGTCAAGA TCTTCACTGC CGACATCATC
    TACCACTTGT TCGACTCGTT CACCAAGCAC ATGGATGAGC TGCTCGAGAA AAAGAAGGAG
    GAGTCCAAGC TCCTCGCTGT CTTCCCTTGC GTGCTCGCTC CCGTTGCCGT CTTCAACAAG
    TCTGGTCCCA TTGTTATCGG TGTCGATGTC GTGGAGGGTC AGTTGAAGAT CAACACGCCC
    ATTTCCGCCG TCAAGACGAA CCCCGTTACT GGCGCTAGGG AGGTCATCGA TCTCGGTAGA
    GTTACCTCCA TCGAGCGCGA CCACAAGCAG ATCCCCGTCT GCAAAAAGGG TCAACCTTCC
    GTCGCCATCA AGATTGAAAT GGGCGGCAAC CAGCCCACGT ACGGCCGTCA TCTAGAGGAG
    AAGGACATGC TCTACTCCAA GATCTCGCGT GCCTCGATCA ACTGCCTCAA GGAGTTTTAC
    CGCAAAGACG TGACCAACGA CGAGTGGCAG CTGATTATCA AGTTGAAGCC CATGTTTGAC
    ATTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_961727.1
    CDS289..3597
    Translation

    Target ORF information:

    RefSeq Version XM_956634.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A mitochondrial translation initiation factor IF-2 (NCU05270), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_956634.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGCCCA AGAAGAAGGG AAACAAGAAG GCCAACGATG ACTGGGAGGC CGAGCTGGGC 
    GAGTCCATCG CCCCCGTCAA CAACAACGGC GCCGATGCTG CTGCCGCCTC TCCCGCTGCC
    GCCGACGACG AGGCCGCTTC CGGTGGTGGC GGTCTGATGG AGAGAATGCG CAAGGCCAAG
    GAGAAGCGCA AGAAGAAGGG TCTTGCCGAC GACTGGGAGG CTGAGCTGGG CGAAGACGCT
    CCCGGCGCCG AGAAGCCCGC TGCCGCCGAG CCCGTCCCCG ATCTGTCGGC CAAGGTCGCC
    GAGGAGGCCA ACCTCGACGA CGAGTTCGCT CTCCCCGAGA AGAAGGGCAA GGGTGGCAAG
    GGCAAGCAGC AGCAGCAGCA ACAGAAGGCT GCTCCCGCCG CCGCCGCCAA GAAGGACGAG
    GATGCTGCCG ATGAGGATGG CCCTAGGGTG TTGACCAAGG CCGAGAAGGA GAAGCTCAAG
    AAGGAGCGTG AGAAGCAGCG CAAGAAGGAG CAGGCTGCTG CTAAGAAGAA GGGCACCACA
    CCCGCCGCCG CCGCCAAGGC CGAGCCCGCA AAGGAGGAGA AGAAGGCCGA AACCCCCAGC
    CCCGTCTCGA CCCCCGCTTC CACTCCCGCC CCCGAAGCCG CCGCCGGTGG CAAGAAGAAG
    AAGCTCCCCG CCCACCTGCT CGCCCTCCAG AAGCAGCAGG AGGAGCTGCG CCGTCGCCAA
    GAGGAGGAGG CTAGGCTCCT CGCCGAGGAG AAGGCCAGGA TAGAGGAGGA GGAGCGCGCC
    GCTGCCGAGG AGGCCAAGCG CAAGGAGGAG GAGCGCGCCC GCAAGAAGGA GAAGGAGAAG
    CAGAGGATCG AGCAGCTCAA GAAGGAGGGC AAGTTCATGA CCAAGGCCCA GAAGGAGGAG
    AAGGCCCGCA ACGAGAGGAA GCTGCAGCAG ATGTTGGCGG CCGGTATCAA GGTCAACGCG
    CTCGAGGGTG AAGAGTCCAA GAAGAAGCCT GTCTACGACG ACAAGAAGAA GAGGGCTGCT
    AACAAGAAGA AGGACGAGGA AAAGGCTCTC GCCGAGGCCG CTGAGCGCGC AAGACTCGCC
    GCTGAGGCTC TGGTCAAGGA GCAAGAGGAG AAGGCCCGCA TCGAGCGCGA GAAGGCCGAG
    AAGGAGGCTG CTGCCAAGGC TGAGGCCGCC AAGGCCGCCG CTGAGGACAG CGTCGACGAG
    GACTGGGAGG CCGCTGTTGC CTCTGACAAG GAGGATGTCA AGGACAGCTG GGACGCCGAC
    TCCGACGAGG AGGAGGAGAC CAAGAAGGAA GAGACCAAGA AGGAGGCTTC TAAGCCCGCC
    GCGGCCAACG GCAAGACTCA GGCCAAGAAG GAGGAGTCCG AGTCCGAGTC CGAGTCTGAG
    GAGGAATCTG AAGAGGAATC TTCTGAAGAC GAGGAGGCTA CCGCCAGGGA ACTGGAGGAG
    GCCCGGAGGA AGAAGGAGGC TGCCGAGCGC CGCGAGAAGG CTCACCAGGC CGCTCTGGCT
    GCCAGGTCTG CGGACAACCT CCGCTCGCCT ATTTGCTGTA TTCTTGGTCA CGTCGATACT
    GGTAAGACGA AGCTGCTCGA CAAGATTCGT CAGACCAACG TCCAGGAGGG TGAAGCCGGT
    GGTATCACTC AGCAGATTGG TGCCACTTAC TTCCCCGTTG AGGCCATCAA GCAGAAGACG
    CACGTCGTCA ACAGGGACGG CAAGTTCGAG TTCAAGGTTC CCGGTCTTCT TATCATCGAT
    ACCCCCGGTC ACGAGTCTTT CTCCAACTTG CGTTCGCGTG GTTCTTCGCT CTGCAACATC
    GCCATCTTGG TTGTCGATAT CATGCACGGT CTCGAACCCC AGACTCTCGA GTCCATGAGA
    CTGCTCCGTG AGAGGAAGAC GCCCTTCATC GTCGCTCTCA ACAAGATCGA TCGTCTTTAC
    GGCTGGAAGA AGATCGAGAA CAACGGCTTC CAGGAGTCGT TGGCCCTCCA GAACAAGGCT
    GTCCAGAACG AGTTCAAGAA CCGTCTCGAG CAGACCAAGC TCGCCTTCGC CGAGCAGGGT
    TTCAACTCGG AGCTCTTCTA CCAGAACAAG TCCATGGCCA AATACGTCTC CTTGGTCCCC
    ACCTCTGCCC ACACCGGCGA GGGTATCCCC GACATGTTGA AGCTCATTGT CCAGCTTACC
    CAGGAGCGCA TGGTTGGCTC TCTCATGTAC CTCTCCGAGG TCCAGGCTAC CGTTCTCGAA
    GTCAAGGCCA TCGAGGGTTT CGGTATGACC ATCGATGTCA TTCTCACCAA CGGTATCCTC
    AGGGAAGGTG ACCGCATTAT CCTATGTGGT ACAGAAGGCG TCATCAAAAC CAATATCAGA
    GCCCTGCTCA CGCCCGCGCC GCTCAGGGAA CTGCGCCTCA AGTCGCAGTA CGTTCACAAT
    AAGGAAGTCA AGGCCGCTTT GGGTGTCAAG ATCTCTGCCC CCGGCCTCGA GGGTGCCATT
    GCCGGTTCCC GTCTGCTTGT CGTCGGCCCC GACGACGACG AGGACGATCT CGAGGACGAG
    GTCGAGGCTG ATCTCGCCTC GCTCTTCAGC CGTGTCGAAA AGTCCGGCCG CGGTGTCTCC
    GTCCAGGCCT CCACCCTTGG TTCGCTCGAG GCCTTGCTCG ACTTCCTCAA GGAGTGCAAG
    ATCCCCGTCG CCAACGTCGG TATCGGTCCC GTCTACAAGC GTGATGTCAT GCAGTGCGGT
    ATCATGTTGG AGAAGGCTCC CGATTATGCC GTCATGCTCT GCTTCGATGT TAAGGTCGAC
    AAGGAAGCGC AGGAGTATGC CGATCAGCAG GGCGTCAAGA TCTTCACTGC CGACATCATC
    TACCACTTGT TCGACTCGTT CACCAAGCAC ATGGATGAGC TGCTCGAGAA AAAGAAGGAG
    GAGTCCAAGC TCCTCGCTGT CTTCCCTTGC GTGCTCGCTC CCGTTGCCGT CTTCAACAAG
    TCTGGTCCCA TTGTTATCGG TGTCGATGTC GTGGAGGGTC AGTTGAAGAT CAACACGCCC
    ATTTCCGCCG TCAAGACGAA CCCCGTTACT GGCGCTAGGG AGGTCATCGA TCTCGGTAGA
    GTTACCTCCA TCGAGCGCGA CCACAAGCAG ATCCCCGTCT GCAAAAAGGG TCAACCTTCC
    GTCGCCATCA AGATTGAAAT GGGCGGCAAC CAGCCCACGT ACGGCCGTCA TCTAGAGGAG
    AAGGACATGC TCTACTCCAA GATCTCGCGT GCCTCGATCA ACTGCCTCAA GGAGTTTTAC
    CGCAAAGACG TGACCAACGA CGAGTGGCAG CTGATTATCA AGTTGAAGCC CATGTTTGAC
    ATTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B