neg-1 cDNA ORF clone, Neurospora crassa OR74A

The following neg-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the neg-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf04488 XM_952379.2
Latest version!
Neurospora crassa OR74A endo-1,6-beta-D-glucanase (neg-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf04488
    Clone ID Related Accession (Same CDS sequence) XM_952379.2
    Accession Version XM_952379.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product endo-1,6-beta-D-glucanase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_952379.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTATCCCC CAGCCCTCAC CCTCCTCTTA ACTCCAGGAC TAGTCGCTGC AGCGATCCAA 
    CCCCAAGCCT ATGCCTCCTC AGCAGATGGC AGATACAAAC TCTCCTCCTA CAGCGCTCCA
    GTCCGGGGCA CCGGCACTCC GGGATCCAAC TCAACATGGA AGCTCACCAT AGACGACACT
    CCCTCTGGTC GGAAGCAAAC CATCAAAGGC TTCGGCGCCG CCGTCACCGA CTCCACCGTG
    TCCGTCTTCA ACGCCCTCCC CTCCGCCCAA CGCACCGCCC TGCTCAACAC CCTCATGACC
    ACCGCCGGCG CCAATTTCGC CATGATGCGA CACACCATTG CCTCCTCCGA CCTCTCTGCC
    AACCCAGCCT ATTCGTACGA TGACTCCAAC GGCCAAACCG ATCTATCCCT TTCCAACTTC
    AACCTCGGCG GCCGCGGCAA CGCCATGGCC TCGCTACTCG CCGAGATGAG ACGTCTCCAA
    CCCGGCCTCA CCATCCTCGG CTCTCCCTGG AGTCCACCAG GTTGGATGAA GCTCAACCGC
    GCGATTCAAG GCACCACGGT AAACAACAAC CTCGACCACG CCTACGCCTC CCAGTTCGCC
    CAGTACTTCG TCAAATACCT CCAAGCATAC CAAGCCAAGG GCGCCACAAT CGACGCCATC
    ACCATCCAAA ACGAACCGCT CAACTCGCGC GCGCAGATGC CGACCATGTA CATCTACGCG
    GACGAGGCCG GTGATCTCAT TCAGAACAAC ATCGGTCCAG CTCTGCGCAA CGCCGGGCTG
    GATACCAAAA TCTGGGCATA CGACCACAAC ACCGACCAAC CCTCCTACCC GTCCACTGTC
    CTTTCTCGGG CGGGCGGGTA CGTTCCCGCG GTGGCATGGC ATTGCTACGC CAGCTCGCTG
    GACTGGTCTG TCTTGACCAC CTTTCACAAC GCCCACCCGG GCGTGGAGCA GTACATGACT
    GAGTGCTGGA CGTCAGCAAA GCAGCCGACG CCGTGGAACT GGGCTGCAAG CTTCACCATG
    GGACCGCTGC AGAACTGGGC GAGCGGGGTG ACGGCGTGGG TCCTGGGGAC GGATACCAAT
    GATGGGCCGC ACTTGACGGG CAGTGATGCG TGTGATAAGT GCACGGGGCT GGTGACGGTA
    GATGCGGCGG CGGGGACGTA TAATTTAAGG GGAGATTATT ACATGATGGC GCAGTTTTCG
    AAGTTTATGA AGAAGGGCGC CGTGGTGATG AGTGGAACGG GAAGTTGGAC GTATGGGGAT
    GGGAGCGGAC TGGAGAGCGT GGCGGCGACG AATGCGGATG ATGGGAGTAG GGTGGTGGTG
    ATTGAGAATA AGTTTGGGAA CGAGATTTAT GTGACGGTTG AGGCGAAGAG TGGGGAGGTT
    TGGAGCGGGT TAGTTTATAG AAACTCGGTG GTGACTTGGG TTTTGCCGGC TGCAGGGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_957472.1
    CDS172..1614
    Translation

    Target ORF information:

    RefSeq Version XM_952379.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A endo-1,6-beta-D-glucanase (neg-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_952379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTATCCCC CAGCCCTCAC CCTCCTCTTA ACTCCAGGAC TAGTCGCTGC AGCGATCCAA 
    CCCCAAGCCT ATGCCTCCTC AGCAGATGGC AGATACAAAC TCTCCTCCTA CAGCGCTCCA
    GTCCGGGGCA CCGGCACTCC GGGATCCAAC TCAACATGGA AGCTCACCAT AGACGACACT
    CCCTCTGGTC GGAAGCAAAC CATCAAAGGC TTCGGCGCCG CCGTCACCGA CTCCACCGTG
    TCCGTCTTCA ACGCCCTCCC CTCCGCCCAA CGCACCGCCC TGCTCAACAC CCTCATGACC
    ACCGCCGGCG CCAATTTCGC CATGATGCGA CACACCATTG CCTCCTCCGA CCTCTCTGCC
    AACCCAGCCT ATTCGTACGA TGACTCCAAC GGCCAAACCG ATCTATCCCT TTCCAACTTC
    AACCTCGGCG GCCGCGGCAA CGCCATGGCC TCGCTACTCG CCGAGATGAG ACGTCTCCAA
    CCCGGCCTCA CCATCCTCGG CTCTCCCTGG AGTCCACCAG GTTGGATGAA GCTCAACCGC
    GCGATTCAAG GCACCACGGT AAACAACAAC CTCGACCACG CCTACGCCTC CCAGTTCGCC
    CAGTACTTCG TCAAATACCT CCAAGCATAC CAAGCCAAGG GCGCCACAAT CGACGCCATC
    ACCATCCAAA ACGAACCGCT CAACTCGCGC GCGCAGATGC CGACCATGTA CATCTACGCG
    GACGAGGCCG GTGATCTCAT TCAGAACAAC ATCGGTCCAG CTCTGCGCAA CGCCGGGCTG
    GATACCAAAA TCTGGGCATA CGACCACAAC ACCGACCAAC CCTCCTACCC GTCCACTGTC
    CTTTCTCGGG CGGGCGGGTA CGTTCCCGCG GTGGCATGGC ATTGCTACGC CAGCTCGCTG
    GACTGGTCTG TCTTGACCAC CTTTCACAAC GCCCACCCGG GCGTGGAGCA GTACATGACT
    GAGTGCTGGA CGTCAGCAAA GCAGCCGACG CCGTGGAACT GGGCTGCAAG CTTCACCATG
    GGACCGCTGC AGAACTGGGC GAGCGGGGTG ACGGCGTGGG TCCTGGGGAC GGATACCAAT
    GATGGGCCGC ACTTGACGGG CAGTGATGCG TGTGATAAGT GCACGGGGCT GGTGACGGTA
    GATGCGGCGG CGGGGACGTA TAATTTAAGG GGAGATTATT ACATGATGGC GCAGTTTTCG
    AAGTTTATGA AGAAGGGCGC CGTGGTGATG AGTGGAACGG GAAGTTGGAC GTATGGGGAT
    GGGAGCGGAC TGGAGAGCGT GGCGGCGACG AATGCGGATG ATGGGAGTAG GGTGGTGGTG
    ATTGAGAATA AGTTTGGGAA CGAGATTTAT GTGACGGTTG AGGCGAAGAG TGGGGAGGTT
    TGGAGCGGGT TAGTTTATAG AAACTCGGTG GTGACTTGGG TTTTGCCGGC TGCAGGGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B