GBA gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001005742.2	NP_001005742.1	lysosomal acid glucosylceramidase isoform 1 precursor
XM_011509407.1	XP_011507709.1	glucosylceramidase isoform X1
NM_001005742.3	NP_001005742.1	lysosomal acid glucosylceramidase isoform 1 precursor
NM_001005741.3	NP_001005741.1	lysosomal acid glucosylceramidase isoform 1 precursor
NM_000157.3	NP_000148.2	glucosylceramidase isoform 1 precursor
NM_001171812.2	NP_001165283.1	lysosomal acid glucosylceramidase isoform 3
NM_000157.4	NP_000148.2	lysosomal acid glucosylceramidase isoform 1 precursor
XM_006711270.1	XP_006711333.1	glucosylceramidase isoform X1
NM_001005741.2	NP_001005741.1	lysosomal acid glucosylceramidase isoform 1 precursor
NM_001171811.1	NP_001165282.1	lysosomal acid glucosylceramidase isoform 2
NM_001171812.1	NP_001165283.1	lysosomal acid glucosylceramidase isoform 3 precursor
NM_001171811.2	NP_001165282.1	lysosomal acid glucosylceramidase isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22331	NM_001005741.3 Latest version!	Homo sapiens glucosylceramidase beta (GBA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22331	NM_000157.4 Latest version!	Homo sapiens glucosylceramidase beta (GBA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22331	NM_001005742.3 Latest version!	Homo sapiens glucosylceramidase beta (GBA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22331	XM_011509407.1 Latest version!	Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22331	XM_006711270.1 Latest version!	Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22337	NM_001171811.2 Latest version!	Homo sapiens glucosylceramidase beta (GBA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu22347	NM_001171812.2 Latest version!	Homo sapiens glucosylceramidase beta (GBA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00
View Old Accession Versions >>
OHu22331	NM_001005742.2	Homo sapiens glucosylceramidase beta (GBA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22331	NM_000157.3	Homo sapiens glucosylceramidase beta (GBA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22331	NM_001005741.2	Homo sapiens glucosylceramidase beta (GBA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22347	NM_001171812.1	Homo sapiens glucosylceramidase beta (GBA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00
OHu22337	NM_001171811.1	Homo sapiens glucosylceramidase beta (GBA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
Hide Old Accession Versions >>

GBA ( NM_001005742.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005742.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	NM_001005742.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300876.1 and AL713999.28. On Jan 28, 2010 this sequence version replaced NM_001005742.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300876.1, SRR1803614.75009.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005742.1
CDS	407..2017
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001005742.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005742.2

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001005742.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005742.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	NM_001005742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713999.28. On May 17, 2019 this sequence version replaced NM_001005742.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.75009.1, AK300876.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005742.1
CDS	172..1782
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001005742.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005742.3

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001005741.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005741.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	NM_001005741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713999.28. This sequence is a reference standard in the RefSeqGene project. On May 15, 2019 this sequence version replaced NM_001005741.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648487.1, SRR5189652.259202.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005741.1
CDS	191..1801
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001005741.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005741.3

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_000157.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000148.2 Homo sapiens glucosylceramidase beta (GBA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	NM_000157.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	glucosylceramidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC297079.1, BC003356.1 and AL713999.28. On Jan 28, 2010 this sequence version replaced NM_000157.2. Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2 and 3 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.119569.1, SRR1803611.165654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000148.2
CDS	167..1777
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_000157.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000157.3

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_000157.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000148.2 Homo sapiens glucosylceramidase beta (GBA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	NM_000157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC297079.1 and BC003356.1. On Nov 22, 2018 this sequence version replaced NM_000157.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1045848.1, SRR1660807.119569.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000368373.8/ ENSP00000357357.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000148.2
CDS	138..1748
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_000157.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000157.4

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001005741.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005741.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	NM_001005741.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300876.1, AK291911.1 and AL713999.28. This sequence is a reference standard in the RefSeqGene project. On Jan 28, 2010 this sequence version replaced NM_001005741.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648487.1, SRR1803616.192634.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005741.1
CDS	426..2036
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001005741.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005741.2

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( XM_011509407.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507709.1 Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA.

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CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	XM_011509407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	glucosylceramidase isoform X1
Comment

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011507709.1

Translation

MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	XM_011509407.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509407.1

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( XM_006711270.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711333.1 Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA.

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CloneID	OHu22331
Clone ID Related Accession (Same CDS sequence)	NM_001005742.2 , XM_011509407.1 , NM_001005742.3 , NM_001005741.3 , NM_000157.3 , NM_000157.4 , XM_006711270.1 , NM_001005741.2
Accession Version	XM_006711270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	glucosylceramidase isoform X1
Comment

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006711333.1

Translation

MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	XM_006711270.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711270.1

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT 
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA 
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA 
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT 
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT 
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA 
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC 
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT 
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG 
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC 
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG 
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA 
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA 
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC 
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG 
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG 
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC 
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC 
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG 
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA 
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG 
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001171811.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165282.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 4, mRNA.

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CloneID	OHu22337
Clone ID Related Accession (Same CDS sequence)	NM_001171811.1 , NM_001171811.2
Accession Version	NM_001171811.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300876.1, AK300829.1 and AL713999.28. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300829.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA GTATGGGGCC CATCCAGGCT AATCACACGG GCACAGGCCT GCTACTGACC 
CTGCAGCCAG AACAGAAGTT CCAGAAAGTG AAGGGATTTG GAGGGGCCAT GACAGATGCT 
GCTGCTCTCA ACATCCTTGC CCTGTCACCC CCTGCCCAAA ATTTGCTACT TAAATCGTAC 
TTCTCTGAAG AAGGAATCGG ATATAACATC ATCCGGGTAC CCATGGCCAG CTGTGACTTC 
TCCATCCGCA CCTACACCTA TGCAGACACC CCTGATGATT TCCAGTTGCA CAACTTCAGC 
CTCCCAGAGG AAGATACCAA GCTCAAGATA CCCCTGATTC ACCGAGCCCT GCAGTTGGCC 
CAGCGTCCCG TTTCACTCCT TGCCAGCCCC TGGACATCAC CCACTTGGCT CAAGACCAAT 
GGAGCGGTGA ATGGGAAGGG GTCACTCAAG GGACAGCCCG GAGACATCTA CCACCAGACC 
TGGGCCAGAT ACTTTGTGAA GTTCCTGGAT GCCTATGCTG AGCACAAGTT ACAGTTCTGG 
GCAGTGACAG CTGAAAATGA GCCTTCTGCT GGGCTGTTGA GTGGATACCC CTTCCAGTGC 
CTGGGCTTCA CCCCTGAACA TCAGCGAGAC TTCATTGCCC GTGACCTAGG TCCTACCCTC 
GCCAACAGTA CTCACCACAA TGTCCGCCTA CTCATGCTGG ATGACCAACG CTTGCTGCTG 
CCCCACTGGG CAAAGGTGGT ACTGACAGAC CCAGAAGCAG CTAAATATGT TCATGGCATT 
GCTGTACATT GGTACCTGGA CTTTCTGGCT CCAGCCAAAG CCACCCTAGG GGAGACACAC 
CGCCTGTTCC CCAACACCAT GCTCTTTGCC TCAGAGGCCT GTGTGGGCTC CAAGTTCTGG 
GAGCAGAGTG TGCGGCTAGG CTCCTGGGAT CGAGGGATGC AGTACAGCCA CAGCATCATC 
ACGAACCTCC TGTACCATGT GGTCGGCTGG ACCGACTGGA ACCTTGCCCT GAACCCCGAA 
GGAGGACCCA ATTGGGTGCG TAACTTTGTC GACAGTCCCA TCATTGTAGA CATCACCAAG 
GACACGTTTT ACAAACAGCC CATGTTCTAC CACCTTGGCC ACTTCAGCAA GTTCATTCCT 
GAGGGCTCCC AGAGAGTGGG GCTGGTTGCC AGTCAGAAGA ACGACCTGGA CGCAGTGGCA 
CTGATGCATC CCGATGGCTC TGCTGTTGTG GTCGTGCTAA ACCGCTCCTC TAAGGATGTG 
CCTCTTACCA TCAAGGATCC TGCTGTGGGC TTCCTGGAGA CAATCTCACC TGGCTACTCC 
ATTCACACCT ACCTGTGGCG TCGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165282.1
CDS	504..1853
Translation	MELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001171811.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171811.1

ORF Insert Sequence:

ATGGAGCTGA GTATGGGGCC CATCCAGGCT AATCACACGG GCACAGGCCT GCTACTGACC 
CTGCAGCCAG AACAGAAGTT CCAGAAAGTG AAGGGATTTG GAGGGGCCAT GACAGATGCT 
GCTGCTCTCA ACATCCTTGC CCTGTCACCC CCTGCCCAAA ATTTGCTACT TAAATCGTAC 
TTCTCTGAAG AAGGAATCGG ATATAACATC ATCCGGGTAC CCATGGCCAG CTGTGACTTC 
TCCATCCGCA CCTACACCTA TGCAGACACC CCTGATGATT TCCAGTTGCA CAACTTCAGC 
CTCCCAGAGG AAGATACCAA GCTCAAGATA CCCCTGATTC ACCGAGCCCT GCAGTTGGCC 
CAGCGTCCCG TTTCACTCCT TGCCAGCCCC TGGACATCAC CCACTTGGCT CAAGACCAAT 
GGAGCGGTGA ATGGGAAGGG GTCACTCAAG GGACAGCCCG GAGACATCTA CCACCAGACC 
TGGGCCAGAT ACTTTGTGAA GTTCCTGGAT GCCTATGCTG AGCACAAGTT ACAGTTCTGG 
GCAGTGACAG CTGAAAATGA GCCTTCTGCT GGGCTGTTGA GTGGATACCC CTTCCAGTGC 
CTGGGCTTCA CCCCTGAACA TCAGCGAGAC TTCATTGCCC GTGACCTAGG TCCTACCCTC 
GCCAACAGTA CTCACCACAA TGTCCGCCTA CTCATGCTGG ATGACCAACG CTTGCTGCTG 
CCCCACTGGG CAAAGGTGGT ACTGACAGAC CCAGAAGCAG CTAAATATGT TCATGGCATT 
GCTGTACATT GGTACCTGGA CTTTCTGGCT CCAGCCAAAG CCACCCTAGG GGAGACACAC 
CGCCTGTTCC CCAACACCAT GCTCTTTGCC TCAGAGGCCT GTGTGGGCTC CAAGTTCTGG 
GAGCAGAGTG TGCGGCTAGG CTCCTGGGAT CGAGGGATGC AGTACAGCCA CAGCATCATC 
ACGAACCTCC TGTACCATGT GGTCGGCTGG ACCGACTGGA ACCTTGCCCT GAACCCCGAA 
GGAGGACCCA ATTGGGTGCG TAACTTTGTC GACAGTCCCA TCATTGTAGA CATCACCAAG 
GACACGTTTT ACAAACAGCC CATGTTCTAC CACCTTGGCC ACTTCAGCAA GTTCATTCCT 
GAGGGCTCCC AGAGAGTGGG GCTGGTTGCC AGTCAGAAGA ACGACCTGGA CGCAGTGGCA 
CTGATGCATC CCGATGGCTC TGCTGTTGTG GTCGTGCTAA ACCGCTCCTC TAAGGATGTG 
CCTCTTACCA TCAAGGATCC TGCTGTGGGC TTCCTGGAGA CAATCTCACC TGGCTACTCC 
ATTCACACCT ACCTGTGGCG TCGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001171811.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165282.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 4, mRNA.

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CloneID	OHu22337
Clone ID Related Accession (Same CDS sequence)	NM_001171811.1 , NM_001171811.2
Accession Version	NM_001171811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713999.28. On May 15, 2019 this sequence version replaced NM_001171811.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300829.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA GTATGGGGCC CATCCAGGCT AATCACACGG GCACAGGCCT GCTACTGACC 
CTGCAGCCAG AACAGAAGTT CCAGAAAGTG AAGGGATTTG GAGGGGCCAT GACAGATGCT 
GCTGCTCTCA ACATCCTTGC CCTGTCACCC CCTGCCCAAA ATTTGCTACT TAAATCGTAC 
TTCTCTGAAG AAGGAATCGG ATATAACATC ATCCGGGTAC CCATGGCCAG CTGTGACTTC 
TCCATCCGCA CCTACACCTA TGCAGACACC CCTGATGATT TCCAGTTGCA CAACTTCAGC 
CTCCCAGAGG AAGATACCAA GCTCAAGATA CCCCTGATTC ACCGAGCCCT GCAGTTGGCC 
CAGCGTCCCG TTTCACTCCT TGCCAGCCCC TGGACATCAC CCACTTGGCT CAAGACCAAT 
GGAGCGGTGA ATGGGAAGGG GTCACTCAAG GGACAGCCCG GAGACATCTA CCACCAGACC 
TGGGCCAGAT ACTTTGTGAA GTTCCTGGAT GCCTATGCTG AGCACAAGTT ACAGTTCTGG 
GCAGTGACAG CTGAAAATGA GCCTTCTGCT GGGCTGTTGA GTGGATACCC CTTCCAGTGC 
CTGGGCTTCA CCCCTGAACA TCAGCGAGAC TTCATTGCCC GTGACCTAGG TCCTACCCTC 
GCCAACAGTA CTCACCACAA TGTCCGCCTA CTCATGCTGG ATGACCAACG CTTGCTGCTG 
CCCCACTGGG CAAAGGTGGT ACTGACAGAC CCAGAAGCAG CTAAATATGT TCATGGCATT 
GCTGTACATT GGTACCTGGA CTTTCTGGCT CCAGCCAAAG CCACCCTAGG GGAGACACAC 
CGCCTGTTCC CCAACACCAT GCTCTTTGCC TCAGAGGCCT GTGTGGGCTC CAAGTTCTGG 
GAGCAGAGTG TGCGGCTAGG CTCCTGGGAT CGAGGGATGC AGTACAGCCA CAGCATCATC 
ACGAACCTCC TGTACCATGT GGTCGGCTGG ACCGACTGGA ACCTTGCCCT GAACCCCGAA 
GGAGGACCCA ATTGGGTGCG TAACTTTGTC GACAGTCCCA TCATTGTAGA CATCACCAAG 
GACACGTTTT ACAAACAGCC CATGTTCTAC CACCTTGGCC ACTTCAGCAA GTTCATTCCT 
GAGGGCTCCC AGAGAGTGGG GCTGGTTGCC AGTCAGAAGA ACGACCTGGA CGCAGTGGCA 
CTGATGCATC CCGATGGCTC TGCTGTTGTG GTCGTGCTAA ACCGCTCCTC TAAGGATGTG 
CCTCTTACCA TCAAGGATCC TGCTGTGGGC TTCCTGGAGA CAATCTCACC TGGCTACTCC 
ATTCACACCT ACCTGTGGCG TCGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165282.1
CDS	269..1618
Translation	MELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001171811.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171811.2

ORF Insert Sequence:

ATGGAGCTGA GTATGGGGCC CATCCAGGCT AATCACACGG GCACAGGCCT GCTACTGACC 
CTGCAGCCAG AACAGAAGTT CCAGAAAGTG AAGGGATTTG GAGGGGCCAT GACAGATGCT 
GCTGCTCTCA ACATCCTTGC CCTGTCACCC CCTGCCCAAA ATTTGCTACT TAAATCGTAC 
TTCTCTGAAG AAGGAATCGG ATATAACATC ATCCGGGTAC CCATGGCCAG CTGTGACTTC 
TCCATCCGCA CCTACACCTA TGCAGACACC CCTGATGATT TCCAGTTGCA CAACTTCAGC 
CTCCCAGAGG AAGATACCAA GCTCAAGATA CCCCTGATTC ACCGAGCCCT GCAGTTGGCC 
CAGCGTCCCG TTTCACTCCT TGCCAGCCCC TGGACATCAC CCACTTGGCT CAAGACCAAT 
GGAGCGGTGA ATGGGAAGGG GTCACTCAAG GGACAGCCCG GAGACATCTA CCACCAGACC 
TGGGCCAGAT ACTTTGTGAA GTTCCTGGAT GCCTATGCTG AGCACAAGTT ACAGTTCTGG 
GCAGTGACAG CTGAAAATGA GCCTTCTGCT GGGCTGTTGA GTGGATACCC CTTCCAGTGC 
CTGGGCTTCA CCCCTGAACA TCAGCGAGAC TTCATTGCCC GTGACCTAGG TCCTACCCTC 
GCCAACAGTA CTCACCACAA TGTCCGCCTA CTCATGCTGG ATGACCAACG CTTGCTGCTG 
CCCCACTGGG CAAAGGTGGT ACTGACAGAC CCAGAAGCAG CTAAATATGT TCATGGCATT 
GCTGTACATT GGTACCTGGA CTTTCTGGCT CCAGCCAAAG CCACCCTAGG GGAGACACAC 
CGCCTGTTCC CCAACACCAT GCTCTTTGCC TCAGAGGCCT GTGTGGGCTC CAAGTTCTGG 
GAGCAGAGTG TGCGGCTAGG CTCCTGGGAT CGAGGGATGC AGTACAGCCA CAGCATCATC 
ACGAACCTCC TGTACCATGT GGTCGGCTGG ACCGACTGGA ACCTTGCCCT GAACCCCGAA 
GGAGGACCCA ATTGGGTGCG TAACTTTGTC GACAGTCCCA TCATTGTAGA CATCACCAAG 
GACACGTTTT ACAAACAGCC CATGTTCTAC CACCTTGGCC ACTTCAGCAA GTTCATTCCT 
GAGGGCTCCC AGAGAGTGGG GCTGGTTGCC AGTCAGAAGA ACGACCTGGA CGCAGTGGCA 
CTGATGCATC CCGATGGCTC TGCTGTTGTG GTCGTGCTAA ACCGCTCCTC TAAGGATGTG 
CCTCTTACCA TCAAGGATCC TGCTGTGGGC TTCCTGGAGA CAATCTCACC TGGCTACTCC 
ATTCACACCT ACCTGTGGCG TCGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001171812.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165283.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 5, mRNA.

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CloneID	OHu22347
Clone ID Related Accession (Same CDS sequence)	NM_001171812.2 , NM_001171812.1
Accession Version	NM_001171812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713999.28. On May 15, 2019 this sequence version replaced NM_001171812.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.162373.1, SRR1803615.44028.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGAA TCGGATATAA CATCATCCGG GTACCCATGG CCAGCTGTGA CTTCTCCATC 
CGCACCTACA CCTATGCAGA CACCCCTGAT GATTTCCAGT TGCACAACTT CAGCCTCCCA 
GAGGAAGATA CCAAGCTCAA GATACCCCTG ATTCACCGAG CCCTGCAGTT GGCCCAGCGT 
CCCGTTTCAC TCCTTGCCAG CCCCTGGACA TCACCCACTT GGCTCAAGAC CAATGGAGCG 
GTGAATGGGA AGGGGTCACT CAAGGGACAG CCCGGAGACA TCTACCACCA GACCTGGGCC 
AGATACTTTG TGAAGTTCCT GGATGCCTAT GCTGAGCACA AGTTACAGTT CTGGGCAGTG 
ACAGCTGAAA ATGAGCCTTC TGCTGGGCTG TTGAGTGGAT ACCCCTTCCA GTGCCTGGGC 
TTCACCCCTG AACATCAGCG AGACTTCATT GCCCGTGACC TAGGTCCTAC CCTCGCCAAC 
AGTACTCACC ACAATGTCCG CCTACTCATG CTGGATGACC AACGCTTGCT GCTGCCCCAC 
TGGGCAAAGG TGGTACTGAC AGACCCAGAA GCAGCTAAAT ATGTTCATGG CATTGCTGTA 
CATTGGTACC TGGACTTTCT GGCTCCAGCC AAAGCCACCC TAGGGGAGAC ACACCGCCTG 
TTCCCCAACA CCATGCTCTT TGCCTCAGAG GCCTGTGTGG GCTCCAAGTT CTGGGAGCAG 
AGTGTGCGGC TAGGCTCCTG GGATCGAGGG ATGCAGTACA GCCACAGCAT CATCACGAAC 
CTCCTGTACC ATGTGGTCGG CTGGACCGAC TGGAACCTTG CCCTGAACCC CGAAGGAGGA 
CCCAATTGGG TGCGTAACTT TGTCGACAGT CCCATCATTG TAGACATCAC CAAGGACACG 
TTTTACAAAC AGCCCATGTT CTACCACCTT GGCCACTTCA GCAAGTTCAT TCCTGAGGGC 
TCCCAGAGAG TGGGGCTGGT TGCCAGTCAG AAGAACGACC TGGACGCAGT GGCACTGATG 
CATCCCGATG GCTCTGCTGT TGTGGTCGTG CTAAACCGCT CCTCTAAGGA TGTGCCTCTT 
ACCATCAAGG ATCCTGCTGT GGGCTTCCTG GAGACAATCT CACCTGGCTA CTCCATTCAC 
ACCTACCTGT GGCGTCGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165283.1
CDS	138..1601
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001171812.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171812.2

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGAA TCGGATATAA CATCATCCGG GTACCCATGG CCAGCTGTGA CTTCTCCATC 
CGCACCTACA CCTATGCAGA CACCCCTGAT GATTTCCAGT TGCACAACTT CAGCCTCCCA 
GAGGAAGATA CCAAGCTCAA GATACCCCTG ATTCACCGAG CCCTGCAGTT GGCCCAGCGT 
CCCGTTTCAC TCCTTGCCAG CCCCTGGACA TCACCCACTT GGCTCAAGAC CAATGGAGCG 
GTGAATGGGA AGGGGTCACT CAAGGGACAG CCCGGAGACA TCTACCACCA GACCTGGGCC 
AGATACTTTG TGAAGTTCCT GGATGCCTAT GCTGAGCACA AGTTACAGTT CTGGGCAGTG 
ACAGCTGAAA ATGAGCCTTC TGCTGGGCTG TTGAGTGGAT ACCCCTTCCA GTGCCTGGGC 
TTCACCCCTG AACATCAGCG AGACTTCATT GCCCGTGACC TAGGTCCTAC CCTCGCCAAC 
AGTACTCACC ACAATGTCCG CCTACTCATG CTGGATGACC AACGCTTGCT GCTGCCCCAC 
TGGGCAAAGG TGGTACTGAC AGACCCAGAA GCAGCTAAAT ATGTTCATGG CATTGCTGTA 
CATTGGTACC TGGACTTTCT GGCTCCAGCC AAAGCCACCC TAGGGGAGAC ACACCGCCTG 
TTCCCCAACA CCATGCTCTT TGCCTCAGAG GCCTGTGTGG GCTCCAAGTT CTGGGAGCAG 
AGTGTGCGGC TAGGCTCCTG GGATCGAGGG ATGCAGTACA GCCACAGCAT CATCACGAAC 
CTCCTGTACC ATGTGGTCGG CTGGACCGAC TGGAACCTTG CCCTGAACCC CGAAGGAGGA 
CCCAATTGGG TGCGTAACTT TGTCGACAGT CCCATCATTG TAGACATCAC CAAGGACACG 
TTTTACAAAC AGCCCATGTT CTACCACCTT GGCCACTTCA GCAAGTTCAT TCCTGAGGGC 
TCCCAGAGAG TGGGGCTGGT TGCCAGTCAG AAGAACGACC TGGACGCAGT GGCACTGATG 
CATCCCGATG GCTCTGCTGT TGTGGTCGTG CTAAACCGCT CCTCTAAGGA TGTGCCTCTT 
ACCATCAAGG ATCCTGCTGT GGGCTTCCTG GAGACAATCT CACCTGGCTA CTCCATTCAC 
ACCTACCTGT GGCGTCGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBA ( NM_001171812.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165283.1 Homo sapiens glucosylceramidase beta (GBA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22347
Clone ID Related Accession (Same CDS sequence)	NM_001171812.2 , NM_001171812.1
Accession Version	NM_001171812.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	lysosomal acid glucosylceramidase isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298900.1 and AL713999.28. Transcript Variant: This variant (5) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298900.1, SRR1660807.23369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGAA TCGGATATAA CATCATCCGG GTACCCATGG CCAGCTGTGA CTTCTCCATC 
CGCACCTACA CCTATGCAGA CACCCCTGAT GATTTCCAGT TGCACAACTT CAGCCTCCCA 
GAGGAAGATA CCAAGCTCAA GATACCCCTG ATTCACCGAG CCCTGCAGTT GGCCCAGCGT 
CCCGTTTCAC TCCTTGCCAG CCCCTGGACA TCACCCACTT GGCTCAAGAC CAATGGAGCG 
GTGAATGGGA AGGGGTCACT CAAGGGACAG CCCGGAGACA TCTACCACCA GACCTGGGCC 
AGATACTTTG TGAAGTTCCT GGATGCCTAT GCTGAGCACA AGTTACAGTT CTGGGCAGTG 
ACAGCTGAAA ATGAGCCTTC TGCTGGGCTG TTGAGTGGAT ACCCCTTCCA GTGCCTGGGC 
TTCACCCCTG AACATCAGCG AGACTTCATT GCCCGTGACC TAGGTCCTAC CCTCGCCAAC 
AGTACTCACC ACAATGTCCG CCTACTCATG CTGGATGACC AACGCTTGCT GCTGCCCCAC 
TGGGCAAAGG TGGTACTGAC AGACCCAGAA GCAGCTAAAT ATGTTCATGG CATTGCTGTA 
CATTGGTACC TGGACTTTCT GGCTCCAGCC AAAGCCACCC TAGGGGAGAC ACACCGCCTG 
TTCCCCAACA CCATGCTCTT TGCCTCAGAG GCCTGTGTGG GCTCCAAGTT CTGGGAGCAG 
AGTGTGCGGC TAGGCTCCTG GGATCGAGGG ATGCAGTACA GCCACAGCAT CATCACGAAC 
CTCCTGTACC ATGTGGTCGG CTGGACCGAC TGGAACCTTG CCCTGAACCC CGAAGGAGGA 
CCCAATTGGG TGCGTAACTT TGTCGACAGT CCCATCATTG TAGACATCAC CAAGGACACG 
TTTTACAAAC AGCCCATGTT CTACCACCTT GGCCACTTCA GCAAGTTCAT TCCTGAGGGC 
TCCCAGAGAG TGGGGCTGGT TGCCAGTCAG AAGAACGACC TGGACGCAGT GGCACTGATG 
CATCCCGATG GCTCTGCTGT TGTGGTCGTG CTAAACCGCT CCTCTAAGGA TGTGCCTCTT 
ACCATCAAGG ATCCTGCTGT GGGCTTCCTG GAGACAATCT CACCTGGCTA CTCCATTCAC 
ACCTACCTGT GGCGTCGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165283.1
CDS	164..1627
Translation	MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Target ORF information:

RefSeq Version	NM_001171812.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosylceramidase beta (GBA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171812.1

ORF Insert Sequence:

ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC 
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA 
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG 
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG 
GGCACAGGAA TCGGATATAA CATCATCCGG GTACCCATGG CCAGCTGTGA CTTCTCCATC 
CGCACCTACA CCTATGCAGA CACCCCTGAT GATTTCCAGT TGCACAACTT CAGCCTCCCA 
GAGGAAGATA CCAAGCTCAA GATACCCCTG ATTCACCGAG CCCTGCAGTT GGCCCAGCGT 
CCCGTTTCAC TCCTTGCCAG CCCCTGGACA TCACCCACTT GGCTCAAGAC CAATGGAGCG 
GTGAATGGGA AGGGGTCACT CAAGGGACAG CCCGGAGACA TCTACCACCA GACCTGGGCC 
AGATACTTTG TGAAGTTCCT GGATGCCTAT GCTGAGCACA AGTTACAGTT CTGGGCAGTG 
ACAGCTGAAA ATGAGCCTTC TGCTGGGCTG TTGAGTGGAT ACCCCTTCCA GTGCCTGGGC 
TTCACCCCTG AACATCAGCG AGACTTCATT GCCCGTGACC TAGGTCCTAC CCTCGCCAAC 
AGTACTCACC ACAATGTCCG CCTACTCATG CTGGATGACC AACGCTTGCT GCTGCCCCAC 
TGGGCAAAGG TGGTACTGAC AGACCCAGAA GCAGCTAAAT ATGTTCATGG CATTGCTGTA 
CATTGGTACC TGGACTTTCT GGCTCCAGCC AAAGCCACCC TAGGGGAGAC ACACCGCCTG 
TTCCCCAACA CCATGCTCTT TGCCTCAGAG GCCTGTGTGG GCTCCAAGTT CTGGGAGCAG 
AGTGTGCGGC TAGGCTCCTG GGATCGAGGG ATGCAGTACA GCCACAGCAT CATCACGAAC 
CTCCTGTACC ATGTGGTCGG CTGGACCGAC TGGAACCTTG CCCTGAACCC CGAAGGAGGA 
CCCAATTGGG TGCGTAACTT TGTCGACAGT CCCATCATTG TAGACATCAC CAAGGACACG 
TTTTACAAAC AGCCCATGTT CTACCACCTT GGCCACTTCA GCAAGTTCAT TCCTGAGGGC 
TCCCAGAGAG TGGGGCTGGT TGCCAGTCAG AAGAACGACC TGGACGCAGT GGCACTGATG 
CATCCCGATG GCTCTGCTGT TGTGGTCGTG CTAAACCGCT CCTCTAAGGA TGTGCCTCTT 
ACCATCAAGG ATCCTGCTGT GGGCTTCCTG GAGACAATCT CACCTGGCTA CTCCATTCAC 
ACCTACCTGT GGCGTCGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GBA
Entrez Gene ID	2629
Full Name	glucosylceramidase beta
Synonyms	GBA1,GCB,GLUC
General protein information	Preferred Names glucosylceramidase beta Names glucosylceramidase D-glucosyl-N-acylsphingosine glucohydrolase acid beta-glucosidase alglucerase beta-GC beta-glucocerebrosidase glucosidase, beta, acid glucosylceramidase-like protein imiglucerase lysosomal glucocerebrosidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q22
Summary	This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010].
Disorder MIM:	606463
Disorder Html:	Gaucher disease, type I, 230800 (3); Gaucher disease, type II, 230900 (3); Gaucher disease, type III, 231000 (3); Gaucher disease, type IIIC, 231005 (3); Gaucher disease, perinatal lethal, 608013 (3); {Parkinson disease, late-onset, susceptibility to}, 168600 (3); {Lewy body dementia, susceptibility to}, 127750 (3)

Rattus norvegicus (Norway rat)	Gba	NP_001121111.1
Caenorhabditis elegans (roundworm)	gba-3	NP_503119.1
Caenorhabditis elegans (roundworm)	gba-1	NP_001040750.1
Bos taurus (cattle)	GBA	NP_001039886.1
Pan troglodytes (chimpanzee)	GBA	NP_001008997.1
Danio rerio (zebrafish)	gba	XP_687471.3
Caenorhabditis elegans (roundworm)	gba-4	NP_500785.1
Magnaporthe oryzae (rice blast fungus)	MGG_14602	XP_003708898.1
Canis lupus familiaris (dog)	GBA	XP_855035.1
Mus musculus (house mouse)	Gba	NP_001070879.1
Gallus gallus (chicken)	LOC101748171	XP_004950417.1
Caenorhabditis elegans (roundworm)	gba-2	NP_494208.2
Neurospora crassa	NCU04395	XP_957472.1
Homo sapiens (human)	GBA	NP_001005742.1
Gallus gallus (chicken)	LOC101750375	XP_004950434.1

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GBA cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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