Mepe cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mepe
Entrez Gene ID	94111
Full Name	matrix extracellular phosphoglycoprotein with ASARM motif (bone)
Synonyms	Of45
General protein information	Preferred Names matrix extracellular phosphoglycoprotein with ASARM motif (bone) Names matrix extracellular phosphoglycoprotein osteoregulin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_053172.2	NP_444402.2	matrix extracellular phosphoglycoprotein precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mepe	NP_077056.1
Bos taurus (cattle)	MEPE	NP_001179933.2
Pan troglodytes (chimpanzee)	MEPE	XP_003310428.1
Canis lupus familiaris (dog)	MEPE	XP_544973.3
Homo sapiens (human)	MEPE	NP_064588.1
	MEPE	XP_001099140.1
Mus musculus (house mouse)	Mepe	NP_444402.2

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant.
Hai Q, Ritchey B, Robinet P, Alzayed AM, Brubaker G, Zhang J, Smith JD
Arteriosclerosis, thrombosis, and vascular biology38(1)83-91(2018 Jan)

The role of the SIBLING, Bone Sialoprotein in skeletal biology - Contribution of mouse experimental genetics.
Bouleftour W, Juignet L, Bouet G, Granito RN, Vanden-Bossche A, Laroche N, Aubin JE, Lafage-Proust MH, Vico L, Malaval L
Matrix biology : journal of the International Society for Matrix Biology52-5460-77(2016 May-Jul)

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation.
Gullard A, Gluhak-Heinrich J, Papagerakis S, Sohn P, Unterbrink A, Chen S, MacDougall M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society64(4)224-36(2016 04)

An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage.
Fujikawa K, Yokohama-Tamaki T, Morita T, Baba O, Qin C, Shibata S
European journal of histochemistry : EJH59(3)2553(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mepe(-/-) molars exhibited increased thickness of predentin, dentin, and enamel over controls and decreased gene expression of Enam, Bsp, Dmp1, Dspp, and Opn by RT-PCR.
Title: MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation.

The C-terminal ASARM-motif plays a major role in regulating bone-mass and cancellous structure as mice age
Title: Age dependent regulation of bone-mass and renal function by the MEPE ASARM-motif.

An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage.
Title: An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage.

MEPE is an important regulator of growth plate chondrocyte matrix mineralization through its cleavage to an ASARM peptide.
Title: MEPE is a novel regulator of growth plate cartilage mineralization.

Wnt3a stimulates Mepe transcription directly by a canonical Wnt signaling pathway through beta-catenin and Lef-1 and indirectly through the activation of a Bmp-2 autocrine loop.
Title: Wnt3a stimulates Mepe, matrix extracellular phosphoglycoprotein, expression directly by the activation of the canonical Wnt signaling pathway and indirectly through the stimulation of autocrine Bmp-2 expression.

The following Mepe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mepe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu25333	NM_053172.2 Latest version!	Mus musculus matrix extracellular phosphoglycoprotein with ASARM motif (bone) (Mepe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mepe ( NM_053172.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444402.2 Mus musculus matrix extracellular phosphoglycoprotein with ASARM motif (bone) (Mepe), mRNA.

Price & Availability↑

CloneID	OMu25333
Clone ID Related Accession (Same CDS sequence)	NM_053172.2
Accession Version	NM_053172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	matrix extracellular phosphoglycoprotein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF298661.1. On Mar 27, 2009 this sequence version replaced NM_053172.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF298661.1, AK158330.1 [ECO:0000332] ##Evidence-Data-END##

ATGACGCCAG AGGGCCTCAT GAAGATGCAG GCTGTGTCTG TTGGACTGCT CCTCTTCAGT 
ATGACCTGGG CGGCACCAAT GCCGAATGAA GACAGGAGCA GCTGCGGCAA TCAAGACAGC 
ATTCACAAGG ACTTGGCAGC ATCTGTGTAT CCTGATCCCA CGGTGGATGA AGGCACAGAG 
GATGGGCAAG GTGCTCTCCT TCACCCGCCT GGCCAGGACA GGTATGGTGC TGCCCTCCTC 
AGAAATATCA CGCAGCCTGT AAAGAGTCTA GTGACTGGGG CCGAACTACG GAGGGAAGGA 
AACCAGGAGA AGAGACCTCA GAGTGTTCTA AGCGTAATTC CAGCAGATGT CAATGATGCT 
AAAGTCTCCT TAAAAGACAT AAAGAATCAA GAGAGTTATC TGCTAACCCA GAGCAGCCCG 
GTCAAAAGCA AACACACCAA ACACACCCGC CAGACCCGAC GGAGCACTCA CTACCTGACA 
CATCTCCCAC AGATCAAGAA GACTCCCAGT GACCTTGAAG GCAGTGGCTC CCCAGATCTT 
CTAGTGAGGG GAGATAATGA TGTCCCCCCT TTCAGTGGAG ATGGGCAACA TTTTATGCAC 
ATTCCTGGCA AAGGAGGTGC TGGGTCTGGT CCTGAAAGCT CAACTAGTCG CCCCCTCTCA 
GGCTCCAGCA AAGCTGAAGT TATTGACCCA CATATGAGTG GACTAGGCTC TAATGAGATC 
CCGGGGAGAG AAGGACATGG TGGCAGTGCC TATGCAACCA GAGACAAAGC TGCACAGGGG 
GCAGGCTCTG CAGGTGGGAG CCTTGTGGGG GGCAGCAATG AAATCACAGG CAGCACCAAT 
TTCAGGGAAC TCCCCGGAAA AGAAGGAAAC AGAATTAATG CCGGCAGCCA AAATGCTCAT 
CAAGGGAAAG TAGAATTTCA CTATCCACAA GTGGCCTCGA GAGAAAAGGT AAAGGGGGGC 
GTGGAGCATG CAGGGAGAGC TGGTTACAAC GAAATCCCCA AGAGCAGCAA AGGTAGCTCT 
AGCAAAGATG CAGAAGAGTC CAAAGGGAAC CAATTAACCT TGACTGCAAG CCAAAGATTT 
CCAGGTAAAG GCAAAAGCCA GGGCCCTGCT CTGCCCTCTC ACAGTCTTAG TAATGAGGTT 
AAAAGTGAAG AAAACCATTA TGTGTTCCAT GGACAAAATA ATCTTACACC GAATAAAGGG 
ATGTCACAGC GGAGAGGCTC CTGGCCTTCG AGAAGACCCA ATTCCCACAG GCGCGCTAGC 
ACCCGCCAAA GAGACAGCAG CGAGTCGTCA TCCAGTGGGA GTTCTAGTGA GAGTCATGGT 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444402.2
CDS	67..1392
Translation	MTPEGLMKMQAVSVGLLLFSMTWAAPMPNEDRSSCGNQDSIHKDLAASVYPDPTVDEGTEDGQGALLHPPGQDRYGAALLRNITQPVKSLVTGAELRREGNQEKRPQSVLSVIPADVNDAKVSLKDIKNQESYLLTQSSPVKSKHTKHTRQTRRSTHYLTHLPQIKKTPSDLEGSGSPDLLVRGDNDVPPFSGDGQHFMHIPGKGGAGSGPESSTSRPLSGSSKAEVIDPHMSGLGSNEIPGREGHGGSAYATRDKAAQGAGSAGGSLVGGSNEITGSTNFRELPGKEGNRINAGSQNAHQGKVEFHYPQVASREKVKGGVEHAGRAGYNEIPKSSKGSSSKDAEESKGNQLTLTASQRFPGKGKSQGPALPSHSLSNEVKSEENHYVFHGQNNLTPNKGMSQRRGSWPSRRPNSHRRASTRQRDSSESSSSGSSSESHGD

Target ORF information:

RefSeq Version	NM_053172.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix extracellular phosphoglycoprotein with ASARM motif (bone) (Mepe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053172.2

ORF Insert Sequence:

ATGACGCCAG AGGGCCTCAT GAAGATGCAG GCTGTGTCTG TTGGACTGCT CCTCTTCAGT 
ATGACCTGGG CGGCACCAAT GCCGAATGAA GACAGGAGCA GCTGCGGCAA TCAAGACAGC 
ATTCACAAGG ACTTGGCAGC ATCTGTGTAT CCTGATCCCA CGGTGGATGA AGGCACAGAG 
GATGGGCAAG GTGCTCTCCT TCACCCGCCT GGCCAGGACA GGTATGGTGC TGCCCTCCTC 
AGAAATATCA CGCAGCCTGT AAAGAGTCTA GTGACTGGGG CCGAACTACG GAGGGAAGGA 
AACCAGGAGA AGAGACCTCA GAGTGTTCTA AGCGTAATTC CAGCAGATGT CAATGATGCT 
AAAGTCTCCT TAAAAGACAT AAAGAATCAA GAGAGTTATC TGCTAACCCA GAGCAGCCCG 
GTCAAAAGCA AACACACCAA ACACACCCGC CAGACCCGAC GGAGCACTCA CTACCTGACA 
CATCTCCCAC AGATCAAGAA GACTCCCAGT GACCTTGAAG GCAGTGGCTC CCCAGATCTT 
CTAGTGAGGG GAGATAATGA TGTCCCCCCT TTCAGTGGAG ATGGGCAACA TTTTATGCAC 
ATTCCTGGCA AAGGAGGTGC TGGGTCTGGT CCTGAAAGCT CAACTAGTCG CCCCCTCTCA 
GGCTCCAGCA AAGCTGAAGT TATTGACCCA CATATGAGTG GACTAGGCTC TAATGAGATC 
CCGGGGAGAG AAGGACATGG TGGCAGTGCC TATGCAACCA GAGACAAAGC TGCACAGGGG 
GCAGGCTCTG CAGGTGGGAG CCTTGTGGGG GGCAGCAATG AAATCACAGG CAGCACCAAT 
TTCAGGGAAC TCCCCGGAAA AGAAGGAAAC AGAATTAATG CCGGCAGCCA AAATGCTCAT 
CAAGGGAAAG TAGAATTTCA CTATCCACAA GTGGCCTCGA GAGAAAAGGT AAAGGGGGGC 
GTGGAGCATG CAGGGAGAGC TGGTTACAAC GAAATCCCCA AGAGCAGCAA AGGTAGCTCT 
AGCAAAGATG CAGAAGAGTC CAAAGGGAAC CAATTAACCT TGACTGCAAG CCAAAGATTT 
CCAGGTAAAG GCAAAAGCCA GGGCCCTGCT CTGCCCTCTC ACAGTCTTAG TAATGAGGTT 
AAAAGTGAAG AAAACCATTA TGTGTTCCAT GGACAAAATA ATCTTACACC GAATAAAGGG 
ATGTCACAGC GGAGAGGCTC CTGGCCTTCG AGAAGACCCA ATTCCCACAG GCGCGCTAGC 
ACCCGCCAAA GAGACAGCAG CGAGTCGTCA TCCAGTGGGA GTTCTAGTGA GAGTCATGGT 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant.
Arteriosclerosis, thrombosis, and vascular biology38(1)83-91(2018 Jan)
Hai Q,Ritchey B,Robinet P,Alzayed AM,Brubaker G,Zhang J,Smith JD

The role of the SIBLING, Bone Sialoprotein in skeletal biology - Contribution of mouse experimental genetics.
Matrix biology : journal of the International Society for Matrix Biology52-5460-77(2016 May-Jul)
Bouleftour W,Juignet L,Bouet G,Granito RN,Vanden-Bossche A,Laroche N,Aubin JE,Lafage-Proust MH,Vico L,Malaval L

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society64(4)224-36(2016 04)
Gullard A,Gluhak-Heinrich J,Papagerakis S,Sohn P,Unterbrink A,Chen S,MacDougall M

An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage.
European journal of histochemistry : EJH59(3)2553(2015 Sep)
Fujikawa K,Yokohama-Tamaki T,Morita T,Baba O,Qin C,Shibata S