Selenof cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Selenof
Entrez Gene ID	93684
Full Name	selenoprotein F
Synonyms	9430015P09Rik,Sep15
General protein information	Preferred Names selenoprotein F Names selenoprotein F 15 kDa selenoprotein selenoprotein 15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 H2
Summary	The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. Knockout studies in mice also suggest a role for this gene in cataract formation and colon carcinogenesis. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053102.2	NP_444332.1	selenoprotein F precursor

Pathways from BioSystems

Homologies

	SEP15	NP_001152888.1
Bos taurus (cattle)	SEP15	NP_001029931.2
Gallus gallus (chicken)	SEP15	NP_001012944.2
Arabidopsis thaliana (thale cress)	AT1G05720	NP_563747.1
Xenopus tropicalis (tropical clawed frog)	sep15	NP_001165182.1
Homo sapiens (human)	SEP15	NP_004252.2
Pan troglodytes (chimpanzee)	SEP15	NP_001139315.1
Rattus norvegicus (Norway rat)	Sep15	NP_579831.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004986	XP_315090.2
Oryza sativa (rice)	Os01g0894500	NP_001045069.1
Canis lupus familiaris (dog)	SEP15	NP_001108232.1
Mus musculus (house mouse)	Sep15	NP_444332.1
Danio rerio (zebrafish)	sep15	NP_840079.1
Drosophila melanogaster (fruit fly)	CG7484	NP_649000.1
Caenorhabditis elegans (roundworm)	Y76B12C.3	NP_500152.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways.
Tsuji PA, Carlson BA, Yoo MH, Naranjo-Suarez S, Xu XM, He Y, Asaki E, Seifried HE, Reinhold WC, Davis CD, Gladyshev VN, Hatfield DL
PloS one10(4)e0124487(2015)

Knockout of the 15 kDa selenoprotein protects against chemically-induced aberrant crypt formation in mice.
Tsuji PA, Carlson BA, Naranjo-Suarez S, Yoo MH, Xu XM, Fomenko DE, Gladyshev VN, Hatfield DL, Davis CD
PloS one7(12)e50574(2012)

Deficiency in the 15-kDa selenoprotein inhibits tumorigenicity and metastasis of colon cancer cells.
Irons R, Tsuji PA, Carlson BA, Ouyang P, Yoo MH, Xu XM, Hatfield DL, Gladyshev VN, Davis CD
Cancer prevention research (Philadelphia, Pa.)3(5)630-9(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Considering the variable expression levels of Sep15 and TR1 found within the human population, our results provide insights into new roles of selenoproteins in cancer
Title: The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways.

Cataracts resulted from an improper folding status of lens proteins caused by Sep15 deficiency.
Title: Roles of the 15-kDa selenoprotein (Sep15) in redox homeostasis and cataract development revealed by the analysis of Sep 15 knockout mice.

These data suggest tissue specificity in the cancer-protective effects of Sep15 downregulation, which are mediated, at least in part, by influencing the cell cycle.
Title: Deficiency in the 15-kDa selenoprotein inhibits tumorigenicity and metastasis of colon cancer cells.

The cysteine-rich domain of Sep15 exclusively mediates protein-protein interactions with UDP-glucose:glycoprotein glucosyltransferase.
Title: A novel cysteine-rich domain of Sep15 mediates the interaction with UDP-glucose:glycoprotein glucosyltransferase.

Transfecting Sep15 gene did not influence the growth characteristics of BEL-7402 cell line and Sep15 may have redox function.
Title: [Redox reactions of Sep15 and its relationship with tumor development].

The following Selenof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Selenof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18839	NM_053102.2 Latest version!	Mus musculus selenoprotein F (Selenof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Selenof ( NM_053102.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444332.1 Mus musculus selenoprotein F (Selenof), mRNA.

Price & Availability↑

CloneID	OMu18839
Clone ID Related Accession (Same CDS sequence)	NM_053102.2
Accession Version	NM_053102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Mus musculus(house mouse)
Product	selenoprotein F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ134973.1, AC123880.18, BY262067.1, AF288740.1 and AK159085.1. On Jul 28, 2006 this sequence version replaced NM_053102.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF288740.1, AK159085.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10945981 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG GGCAGGGTGG GTGGCTGCGG CCCGCTCTGG GGCTGCGCTT GCTGCTGGCG 
ACTGCGTTTC AAGCGGCGTC TGCTCTGGGG GCAGAGTTTG CGTCAGAGGC ATGCAGAGAG 
TTGGGTTTCT CCAGCAACTT GCTCTGCAGC TCTTGCGATC TTCTTGGACA GTTTAATCTG 
CTCCCACTGG ATCCTGTTTG CAGAGGGTGC TGTCAGGAAG AAGCACAATT TGAAACCAAA 
AAGCTGTATG CAGGAGCCAT CCTTGAAGTC TGCGGATGAA AATTGGGGAG GTTCCCTCAA 
GTCCAAGCTT TTGTCAGAAG TGATAAACCC AAACTCTTCA GAGGTCTACA GATCAAGTAT 
GTTCGAGGCT CAGACCCTGT ACTAAAGCTT TTGGACGACA ACGGGAACAT TGCTGAAGAA 
CTAAGCATCC TCAAGTGGAA CACAGACAGT GTGGAAGAGT TCCTGAGCGA GAAGTTGGAA 
CGCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444332.1
CDS	299..787
Translation	MAAGQGGWLRPALGLRLLLATAFQAASALGAEFASEACRELGFSSNLLCSSCDLLGQFNLLPLDPVCRGCCQEEAQFETKKLYAGAILEVCG*KLGRFPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI

Target ORF information:

RefSeq Version	NM_053102.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus selenoprotein F (Selenof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053102.2

ORF Insert Sequence:

ATGGCGGCAG GGCAGGGTGG GTGGCTGCGG CCCGCTCTGG GGCTGCGCTT GCTGCTGGCG 
ACTGCGTTTC AAGCGGCGTC TGCTCTGGGG GCAGAGTTTG CGTCAGAGGC ATGCAGAGAG 
TTGGGTTTCT CCAGCAACTT GCTCTGCAGC TCTTGCGATC TTCTTGGACA GTTTAATCTG 
CTCCCACTGG ATCCTGTTTG CAGAGGGTGC TGTCAGGAAG AAGCACAATT TGAAACCAAA 
AAGCTGTATG CAGGAGCCAT CCTTGAAGTC TGCGGATGAA AATTGGGGAG GTTCCCTCAA 
GTCCAAGCTT TTGTCAGAAG TGATAAACCC AAACTCTTCA GAGGTCTACA GATCAAGTAT 
GTTCGAGGCT CAGACCCTGT ACTAAAGCTT TTGGACGACA ACGGGAACAT TGCTGAAGAA 
CTAAGCATCC TCAAGTGGAA CACAGACAGT GTGGAAGAGT TCCTGAGCGA GAAGTTGGAA 
CGCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways.
PloS one10(4)e0124487(2015)
Tsuji PA,Carlson BA,Yoo MH,Naranjo-Suarez S,Xu XM,He Y,Asaki E,Seifried HE,Reinhold WC,Davis CD,Gladyshev VN,Hatfield DL

Knockout of the 15 kDa selenoprotein protects against chemically-induced aberrant crypt formation in mice.
PloS one7(12)e50574(2012)
Tsuji PA,Carlson BA,Naranjo-Suarez S,Yoo MH,Xu XM,Fomenko DE,Gladyshev VN,Hatfield DL,Davis CD

Deficiency in the 15-kDa selenoprotein inhibits tumorigenicity and metastasis of colon cancer cells.
Cancer prevention research (Philadelphia, Pa.)3(5)630-9(2010 May)
Irons R,Tsuji PA,Carlson BA,Ouyang P,Yoo MH,Xu XM,Hatfield DL,Gladyshev VN,Davis CD

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Selenof cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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