Specc1l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Specc1l
Entrez Gene ID	74392
Full Name	sperm antigen with calponin homology and coiled-coil domains 1-like
Synonyms	4930470P14Rik,4932439K10Rik,9530057A13Rik,AI317249,Cytsa,mKIAA0376
General protein information	Preferred Names sperm antigen with calponin homology and coiled-coil domains 1-like Names cytospin-A SPECC1-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314127.1	XP_017169616.1	cytospin-A isoform X2
NM_001145826.1	NP_001139298.1	cytospin-A isoform a
XM_030245333.1	XP_030101193.1	cytospin-A isoform X2
XM_006514252.3	XP_006514315.1	cytospin-A isoform X2
XM_011243589.2	XP_011241891.1	cytospin-A isoform X1
XM_006514252.4	XP_006514315.1	cytospin-A isoform X2
NM_153406.3	NP_700455.3	cytospin-A isoform b

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPECC1L	NP_001038102.1
Homo sapiens (human)	SPECC1L	NP_001138940.3
Pan troglodytes (chimpanzee)	SPECC1L	NP_001035052.1
Bos taurus (cattle)	SPECC1L	NP_001192793.1
Canis lupus familiaris (dog)	SPECC1L	NP_001041579.1
Mus musculus (house mouse)	Specc1l	NP_001139298.1
Xenopus tropicalis (tropical clawed frog)	specc1l	NP_001037875.1
	SPECC1L	XP_001094607.1
Rattus norvegicus (Norway rat)	Specc1l	NP_001034544.1
Danio rerio (zebrafish)	specc1la	NP_001034905.1

Gene Ontology
Bibliography

Related articles in PubMed

SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.
Wilson NR, Olm-Shipman AJ, Acevedo DS, Palaniyandi K, Hall EG, Kosa E, Stumpff KM, Smith GJ, Pitstick L, Liao EC, Bjork BC, Czirok A, Saadi I
Scientific reports617735(2016 Jan)

Deficiency of the cytoskeletal protein SPECC1L leads to oblique facial clefting.
Saadi I, Alkuraya FS, Gisselbrecht SS, Goessling W, Cavallesco R, Turbe-Doan A, Petrin AL, Harris J, Siddiqui U, Grix AW Jr, Hove HD, Leboulch P, Glover TW, Morton CC, Richieri-Costa A, Murray JC, Erickson RP, Maas RL
American journal of human genetics89(1)44-55(2011 Jul)

A mutation in the pericentrin gene causes abnormal interneuron migration to the olfactory bulb in mice.
Endoh-Yamagami S, Karkar KM, May SR, Cobos I, Thwin MT, Long JE, Ashique AM, Zarbalis K, Rubenstein JL, Peterson AS
Developmental biology340(1)41-53(2010 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Hara Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes9(5)179-88(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPECC1L as a novel modulator of PI3K-AKT signaling and AJ biology, required for neural tube closure and CNCC delamination.
Title: SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.

The following Specc1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Specc1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02686	XM_030245333.1 Latest version!	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu02686	XM_006514252.4 Latest version!	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu02686	NM_153406.3 Latest version!	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu58421	NM_001145826.1 Latest version!	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu02686	XM_017314127.1 Latest version!	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu58421	XM_011243589.2 Latest version!	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu02686	XM_006514252.3	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Specc1l ( XM_030245333.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101193.1 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu02686
Clone ID Related Accession (Same CDS sequence)	XM_017314127.1 , XM_030245333.1 , XM_006514252.3 , XM_006514252.4 , NM_153406.3
Accession Version	XM_030245333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101193.1
CDS	472..3828
Translation	MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	XM_030245333.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245333.1

ORF Insert Sequence:

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Specc1l ( XM_006514252.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514315.1 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu02686
Clone ID Related Accession (Same CDS sequence)	XM_017314127.1 , XM_030245333.1 , XM_006514252.3 , XM_006514252.4 , NM_153406.3
Accession Version	XM_006514252.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514252.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514315.1
CDS	296..3652
Translation	MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	XM_006514252.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514252.4

ORF Insert Sequence:

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Specc1l ( NM_153406.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_700455.3 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 2, mRNA.

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CloneID	OMu02686
Clone ID Related Accession (Same CDS sequence)	XM_017314127.1 , XM_030245333.1 , XM_006514252.3 , XM_006514252.4 , NM_153406.3
Accession Version	NM_153406.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY884297.1, AK041543.1, BC072595.1 and AI853253.1. On Mar 12, 2009 this sequence version replaced NM_153406.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translational start codon, compared to variant 1. The resulting isoform (b) is shorter at the N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.183653.1, SRR1660817.88982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_700455.3
CDS	185..3541
Translation	MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_153406.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153406.3

ORF Insert Sequence:

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Specc1l ( NM_001145826.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139298.1 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 1, mRNA.

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CloneID	OMu58421
Clone ID Related Accession (Same CDS sequence)	NM_001145826.1 , XM_011243589.2
Accession Version	NM_001145826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB093236.1, BC072595.1 and AI853253.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AB093236.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGAAC TAAGGTTTGG TTGTAAATGC ATCACCAAGA GGCAGCCCAG AATGAAGAAA 
GCAAACAGGA GTGCTGGCTC AGTGCCTAAG GTGTCTGGGA TAAGCAAACC GCAAACAGTA 
GAGAAAAGCA AGCCTGAGAA CAGCTCTTCA GCACCCACAG GAGTCAAGCC TGTGAGGCCT 
GGGGCAGCAG CAGCCTTGTC AAAGACCAAG AGCAATGATG ACCTTTTAGC TGGAATGGCT 
GGAGGAGTAA ACGTGACTAA TGGTATTAAA GCAAAGAAAA GCACCTGTTC ATCTGCAGCG 
CCTTCAGCCC CCGCCCCTGC CATGACCATC TCGGAGAACA AGTCCAAGAT CAGCACAGGC 
ACTAGTTCCT CAGCCAAACG GAGTACTTCG GCTGGTAATA AAGAGTCCAG TTCTACTAGA 
GAAAGATTAC GTGAACGCAC CCGGCTAAAC CAGAGCAAAA AACTGCCCTC TGTAAGTCAG 
GGAGCTAATG ATGTGGCACT GGCTAAACGT TCACGCAGCC GCACTGCTGC AGAGGGTGAT 
ATTCGTATGA GCAAGTCTAA GTCGGACAAT CAGATCAGTG ACAAAGCTGC TTTGGAAGCC 
AAGGTGAAAG ATCTCCTCAC ACTGGCGAAA ACCAAGGATG TGGAAATTTT ACATTTGAGA 
AATGAACTCC GAGACATGCG TGCTCAGCTG GGCATTAGTG AGGACCATTG TGAAGGTGAG 
GACAGGTCTG AGGTGAAGGA AACTATTATT GCTCACCAGC CTACTGACGT GGAGTCTACT 
CTCCTGCAGC TGCAGGAGCA GAATACAGCC ATCCGCGAGG AGCTCAACCA GCTGAAGAAT 
GAAAACAGAA TGCTAAAGGA CAGGCTCAAT GCTTTGGGCT TTTCCCTAGA GCAAAGGCTG 
GACAATTCTG AAAAACTATT TGGCTATCAG TCCCTGAGCC CAGAAATCAC CCCTGGTAAC 
CAGAGTGACG GAGGAGGAAC TCTGACATCT TCGGTAGAAG GTTCTGCTCC TGGGTCGGTG 
GAAGATCTCC TGAGTCAGGA TGAAAATACA CTCATGGACC ATCAGCACAG TAACTCCATG 
GACAACCTGG ACAGTGAGTG CAGCGAGGTG TACCAGCCCC TCACTTCCAG CGATGATGCT 
CTGGACGCCC CCTCCTCTTC AGAGTCAGAG GGCGTGCCAA GCATTGAGCG CTCTCGGAAG 
GGGAGCAGTG GGAATGCCAG TGAGGTGTCC GTCGCTTGCC TGACAGAACG GATTCACCAG 
ATGGAAGAGA ACCAGCACAG CACCAGTGAG GAGCTTCAGG CCACTCTACA GGAGCTGGCT 
GATCTCCAGC AGATTACCCA GGAGCTCAAC AGTGAGAACG AAAGGCTGGG AGAGGAGAAG 
GTCATTCTGA TGGAGTCGCT GTGTCAGCAG AGTGATAAGT TGGAACACTT TGGCCGGCAG 
ATTGAGTATT TCCGTTCCCT TCTTGATGAG CATCACATTT CCTATGTCAT AGATGAAGAC 
GTAAAGAGCG GGCGCTATAT GGAACTAGAG CAGCGCTACA TGGATTTGGC TGAGAATGCC 
CGTTTTGAGC GAGAGCAGCT TCTAGGTGTG CAGCAGCATT TAAGCAACAC TTTGAAGATG 
GCAGAACAAG ATAACAAAGA AGCTCAAGAA ATGATAGGTG CCCTCAAAGA GCGCAGCCAC 
CACATGGAGC GGATTATTGA GTCTGAGCAG AAGGGCAAAG CGGCCCTGGC AGCCACATTG 
GAGGAGTACA AAGCTACTGT GGCCAGTGAC CAGATAGAGA TGAATCGCTT GAAGGCCCAG 
CTGGAGAACG AAAAGCAGAA GGTGGCTGAG TTGTATTCTA TCCATAACTC TGGAGACAAG 
TCCGATATCC AGGATCTCCT GGAGAGTGTC AGGCTGGACA AGGAAAAGGC GGAGACTTTG 
GCCAGTAGCC TGCAGGAAGA TCTGGCTCAT ACCCGAAATG ATGCCAATCG GTTACAGGAC 
ACCATTGCTA AGGTAGAAGA TGAATACCGA GCTTTCCAGG AAGAGGCCAA GAAGCAGATT 
GAAGATTTGA ATATGACATT AGAAAAACTA AGATCAGAGT TGGAGGAAAA GGACACGGAG 
AGGAGTGACA TGAAGGAAAC CATCTTTGAA CTTGAAGATG AGGTGGAGCA GCACCGCGCC 
GTGAAGCTGC ATGACAACCT CATTATCTCT GATCTAGAGA ATACAGTTAA GAAACTCCAA 
GACCAAAAGC ATGACATGGA AAGAGAAATC AAGACACTTC ACAGGAGACT TCGGGAAGAG 
TCTGCAGAAT GGCGGCAATT TCAGGCTGAT CTCCAGACTG CAGTGGTCAT TGCCAATGAT 
ATCAAGTCTG AAGCCCAAGA AGAGATCGGT GATCTAAAGC GACGGCTACA TGAGGCCCAA 
GAGAAAAATG AGAAACTCAC AAAAGAATTG GAGGAAATAA AGTCACGCAA GCAAGAGGAA 
GAGCGTGGCC GGGTGTATAA CTACATGAAT GCTGTTGAAA GAGATTTGGC AGCCTTGAGG 
CAAGGGATGG GACTGAGTAG AAGGTCCTCC ACTTCCTCAG AACCAACTCC CACAGTAAAA 
ACCCTCATCA AGTCTTTCGA CAGTGCATCT CAAGTACCAA ACGCTGCTGC AGCTGCGATC 
CCTCGAACAC CTCTGAGTCC AAGTCCCATG AAAACCCCTC CTGCAGCTGC CGTTTCTCCC 
ATGCAGAGAC ATTCCATAAG TGGTCCAATC TCAACCTCCA AGCCTCTGAC AGCCTTGTCA 
GATAAGAGAT CAAACTATGG AGAGCTTCCT GTACAAGAGC ATCTACTAAG AACATCTTCA 
ACCAGCCGGC CCGCCTCTCT CCCAAGAGTC CCTGCTATGG AAAGTGCCAA GACCATCTCA 
GTGTCTCGAC GGAGTAGTGA AGAAATGAAA CGGGACATCT CTGCATCTGA GGGCGCGTCA 
CCAGCCTCTC TCATGGCTAT GGGCACCACG TCACCACAGC TCTCCCTCTC TTCCTCTCCC 
ACTGCATCTG TCACTCCAAG CACCCGGAGC CGAATAAGGG AAGAAAGGAA AGACCCCCTC 
TCAGCATTGG CAAGAGAATA TGGAGGCTCC AAGAGGAACG CCTTGTTGAA GTGGTGTCAG 
AAGAAAACAG AAGGCTATCA GAATATTGAC ATCACAAACT TCAGCAGCAG CTGGAACGAC 
GGGCTGGCTT TCTGCGCATT GCTTCATACC TATCTCCCAG CCCACATACC ATATCAAGAA 
CTGAACAGTC AAGATAAGAA AAGGAACTTC ACCCTGGCTT TCCAGGCAGC TGAAAGTGTT 
GGAATCAAAT CGACGCTGGA CATCAATGAA ATGGCGCGTA CTGAACGGCC CGACTGGCAG 
AACGTCATGC TGTATGTTAC AGCCATCTAC AAGTACTTTG AAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139298.1
CDS	363..3770
Translation	MLELRFGCKCITKRQPRMKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_001145826.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145826.1

ORF Insert Sequence:

ATGTTGGAAC TAAGGTTTGG TTGTAAATGC ATCACCAAGA GGCAGCCCAG AATGAAGAAA 
GCAAACAGGA GTGCTGGCTC AGTGCCTAAG GTGTCTGGGA TAAGCAAACC GCAAACAGTA 
GAGAAAAGCA AGCCTGAGAA CAGCTCTTCA GCACCCACAG GAGTCAAGCC TGTGAGGCCT 
GGGGCAGCAG CAGCCTTGTC AAAGACCAAG AGCAATGATG ACCTTTTAGC TGGAATGGCT 
GGAGGAGTAA ACGTGACTAA TGGTATTAAA GCAAAGAAAA GCACCTGTTC ATCTGCAGCG 
CCTTCAGCCC CCGCCCCTGC CATGACCATC TCGGAGAACA AGTCCAAGAT CAGCACAGGC 
ACTAGTTCCT CAGCCAAACG GAGTACTTCG GCTGGTAATA AAGAGTCCAG TTCTACTAGA 
GAAAGATTAC GTGAACGCAC CCGGCTAAAC CAGAGCAAAA AACTGCCCTC TGTAAGTCAG 
GGAGCTAATG ATGTGGCACT GGCTAAACGT TCACGCAGCC GCACTGCTGC AGAGGGTGAT 
ATTCGTATGA GCAAGTCTAA GTCGGACAAT CAGATCAGTG ACAAAGCTGC TTTGGAAGCC 
AAGGTGAAAG ATCTCCTCAC ACTGGCGAAA ACCAAGGATG TGGAAATTTT ACATTTGAGA 
AATGAACTCC GAGACATGCG TGCTCAGCTG GGCATTAGTG AGGACCATTG TGAAGGTGAG 
GACAGGTCTG AGGTGAAGGA AACTATTATT GCTCACCAGC CTACTGACGT GGAGTCTACT 
CTCCTGCAGC TGCAGGAGCA GAATACAGCC ATCCGCGAGG AGCTCAACCA GCTGAAGAAT 
GAAAACAGAA TGCTAAAGGA CAGGCTCAAT GCTTTGGGCT TTTCCCTAGA GCAAAGGCTG 
GACAATTCTG AAAAACTATT TGGCTATCAG TCCCTGAGCC CAGAAATCAC CCCTGGTAAC 
CAGAGTGACG GAGGAGGAAC TCTGACATCT TCGGTAGAAG GTTCTGCTCC TGGGTCGGTG 
GAAGATCTCC TGAGTCAGGA TGAAAATACA CTCATGGACC ATCAGCACAG TAACTCCATG 
GACAACCTGG ACAGTGAGTG CAGCGAGGTG TACCAGCCCC TCACTTCCAG CGATGATGCT 
CTGGACGCCC CCTCCTCTTC AGAGTCAGAG GGCGTGCCAA GCATTGAGCG CTCTCGGAAG 
GGGAGCAGTG GGAATGCCAG TGAGGTGTCC GTCGCTTGCC TGACAGAACG GATTCACCAG 
ATGGAAGAGA ACCAGCACAG CACCAGTGAG GAGCTTCAGG CCACTCTACA GGAGCTGGCT 
GATCTCCAGC AGATTACCCA GGAGCTCAAC AGTGAGAACG AAAGGCTGGG AGAGGAGAAG 
GTCATTCTGA TGGAGTCGCT GTGTCAGCAG AGTGATAAGT TGGAACACTT TGGCCGGCAG 
ATTGAGTATT TCCGTTCCCT TCTTGATGAG CATCACATTT CCTATGTCAT AGATGAAGAC 
GTAAAGAGCG GGCGCTATAT GGAACTAGAG CAGCGCTACA TGGATTTGGC TGAGAATGCC 
CGTTTTGAGC GAGAGCAGCT TCTAGGTGTG CAGCAGCATT TAAGCAACAC TTTGAAGATG 
GCAGAACAAG ATAACAAAGA AGCTCAAGAA ATGATAGGTG CCCTCAAAGA GCGCAGCCAC 
CACATGGAGC GGATTATTGA GTCTGAGCAG AAGGGCAAAG CGGCCCTGGC AGCCACATTG 
GAGGAGTACA AAGCTACTGT GGCCAGTGAC CAGATAGAGA TGAATCGCTT GAAGGCCCAG 
CTGGAGAACG AAAAGCAGAA GGTGGCTGAG TTGTATTCTA TCCATAACTC TGGAGACAAG 
TCCGATATCC AGGATCTCCT GGAGAGTGTC AGGCTGGACA AGGAAAAGGC GGAGACTTTG 
GCCAGTAGCC TGCAGGAAGA TCTGGCTCAT ACCCGAAATG ATGCCAATCG GTTACAGGAC 
ACCATTGCTA AGGTAGAAGA TGAATACCGA GCTTTCCAGG AAGAGGCCAA GAAGCAGATT 
GAAGATTTGA ATATGACATT AGAAAAACTA AGATCAGAGT TGGAGGAAAA GGACACGGAG 
AGGAGTGACA TGAAGGAAAC CATCTTTGAA CTTGAAGATG AGGTGGAGCA GCACCGCGCC 
GTGAAGCTGC ATGACAACCT CATTATCTCT GATCTAGAGA ATACAGTTAA GAAACTCCAA 
GACCAAAAGC ATGACATGGA AAGAGAAATC AAGACACTTC ACAGGAGACT TCGGGAAGAG 
TCTGCAGAAT GGCGGCAATT TCAGGCTGAT CTCCAGACTG CAGTGGTCAT TGCCAATGAT 
ATCAAGTCTG AAGCCCAAGA AGAGATCGGT GATCTAAAGC GACGGCTACA TGAGGCCCAA 
GAGAAAAATG AGAAACTCAC AAAAGAATTG GAGGAAATAA AGTCACGCAA GCAAGAGGAA 
GAGCGTGGCC GGGTGTATAA CTACATGAAT GCTGTTGAAA GAGATTTGGC AGCCTTGAGG 
CAAGGGATGG GACTGAGTAG AAGGTCCTCC ACTTCCTCAG AACCAACTCC CACAGTAAAA 
ACCCTCATCA AGTCTTTCGA CAGTGCATCT CAAGTACCAA ACGCTGCTGC AGCTGCGATC 
CCTCGAACAC CTCTGAGTCC AAGTCCCATG AAAACCCCTC CTGCAGCTGC CGTTTCTCCC 
ATGCAGAGAC ATTCCATAAG TGGTCCAATC TCAACCTCCA AGCCTCTGAC AGCCTTGTCA 
GATAAGAGAT CAAACTATGG AGAGCTTCCT GTACAAGAGC ATCTACTAAG AACATCTTCA 
ACCAGCCGGC CCGCCTCTCT CCCAAGAGTC CCTGCTATGG AAAGTGCCAA GACCATCTCA 
GTGTCTCGAC GGAGTAGTGA AGAAATGAAA CGGGACATCT CTGCATCTGA GGGCGCGTCA 
CCAGCCTCTC TCATGGCTAT GGGCACCACG TCACCACAGC TCTCCCTCTC TTCCTCTCCC 
ACTGCATCTG TCACTCCAAG CACCCGGAGC CGAATAAGGG AAGAAAGGAA AGACCCCCTC 
TCAGCATTGG CAAGAGAATA TGGAGGCTCC AAGAGGAACG CCTTGTTGAA GTGGTGTCAG 
AAGAAAACAG AAGGCTATCA GAATATTGAC ATCACAAACT TCAGCAGCAG CTGGAACGAC 
GGGCTGGCTT TCTGCGCATT GCTTCATACC TATCTCCCAG CCCACATACC ATATCAAGAA 
CTGAACAGTC AAGATAAGAA AAGGAACTTC ACCCTGGCTT TCCAGGCAGC TGAAAGTGTT 
GGAATCAAAT CGACGCTGGA CATCAATGAA ATGGCGCGTA CTGAACGGCC CGACTGGCAG 
AACGTCATGC TGTATGTTAC AGCCATCTAC AAGTACTTTG AAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Specc1l ( XM_017314127.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169616.1 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X2, mRNA.

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CloneID	OMu02686
Clone ID Related Accession (Same CDS sequence)	XM_017314127.1 , XM_030245333.1 , XM_006514252.3 , XM_006514252.4 , NM_153406.3
Accession Version	XM_017314127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169616.1
CDS	398..3754
Translation	MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	XM_017314127.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314127.1

ORF Insert Sequence:

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Specc1l ( XM_006514252.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514315.1 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X3, mRNA.

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CloneID	OMu02686
Clone ID Related Accession (Same CDS sequence)	XM_017314127.1 , XM_030245333.1 , XM_006514252.3 , XM_006514252.4 , NM_153406.3
Accession Version	XM_006514252.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514315.1
CDS	297..3653
Translation	MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	XM_006514252.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514252.3

ORF Insert Sequence:

ATGAAGAAAG CAAACAGGAG TGCTGGCTCA GTGCCTAAGG TGTCTGGGAT AAGCAAACCG 
CAAACAGTAG AGAAAAGCAA GCCTGAGAAC AGCTCTTCAG CACCCACAGG AGTCAAGCCT 
GTGAGGCCTG GGGCAGCAGC AGCCTTGTCA AAGACCAAGA GCAATGATGA CCTTTTAGCT 
GGAATGGCTG GAGGAGTAAA CGTGACTAAT GGTATTAAAG CAAAGAAAAG CACCTGTTCA 
TCTGCAGCGC CTTCAGCCCC CGCCCCTGCC ATGACCATCT CGGAGAACAA GTCCAAGATC 
AGCACAGGCA CTAGTTCCTC AGCCAAACGG AGTACTTCGG CTGGTAATAA AGAGTCCAGT 
TCTACTAGAG AAAGATTACG TGAACGCACC CGGCTAAACC AGAGCAAAAA ACTGCCCTCT 
GTAAGTCAGG GAGCTAATGA TGTGGCACTG GCTAAACGTT CACGCAGCCG CACTGCTGCA 
GAGGGTGATA TTCGTATGAG CAAGTCTAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT 
TTGGAAGCCA AGGTGAAAGA TCTCCTCACA CTGGCGAAAA CCAAGGATGT GGAAATTTTA 
CATTTGAGAA ATGAACTCCG AGACATGCGT GCTCAGCTGG GCATTAGTGA GGACCATTGT 
GAAGGTGAGG ACAGGTCTGA GGTGAAGGAA ACTATTATTG CTCACCAGCC TACTGACGTG 
GAGTCTACTC TCCTGCAGCT GCAGGAGCAG AATACAGCCA TCCGCGAGGA GCTCAACCAG 
CTGAAGAATG AAAACAGAAT GCTAAAGGAC AGGCTCAATG CTTTGGGCTT TTCCCTAGAG 
CAAAGGCTGG ACAATTCTGA AAAACTATTT GGCTATCAGT CCCTGAGCCC AGAAATCACC 
CCTGGTAACC AGAGTGACGG AGGAGGAACT CTGACATCTT CGGTAGAAGG TTCTGCTCCT 
GGGTCGGTGG AAGATCTCCT GAGTCAGGAT GAAAATACAC TCATGGACCA TCAGCACAGT 
AACTCCATGG ACAACCTGGA CAGTGAGTGC AGCGAGGTGT ACCAGCCCCT CACTTCCAGC 
GATGATGCTC TGGACGCCCC CTCCTCTTCA GAGTCAGAGG GCGTGCCAAG CATTGAGCGC 
TCTCGGAAGG GGAGCAGTGG GAATGCCAGT GAGGTGTCCG TCGCTTGCCT GACAGAACGG 
ATTCACCAGA TGGAAGAGAA CCAGCACAGC ACCAGTGAGG AGCTTCAGGC CACTCTACAG 
GAGCTGGCTG ATCTCCAGCA GATTACCCAG GAGCTCAACA GTGAGAACGA AAGGCTGGGA 
GAGGAGAAGG TCATTCTGAT GGAGTCGCTG TGTCAGCAGA GTGATAAGTT GGAACACTTT 
GGCCGGCAGA TTGAGTATTT CCGTTCCCTT CTTGATGAGC ATCACATTTC CTATGTCATA 
GATGAAGACG TAAAGAGCGG GCGCTATATG GAACTAGAGC AGCGCTACAT GGATTTGGCT 
GAGAATGCCC GTTTTGAGCG AGAGCAGCTT CTAGGTGTGC AGCAGCATTT AAGCAACACT 
TTGAAGATGG CAGAACAAGA TAACAAAGAA GCTCAAGAAA TGATAGGTGC CCTCAAAGAG 
CGCAGCCACC ACATGGAGCG GATTATTGAG TCTGAGCAGA AGGGCAAAGC GGCCCTGGCA 
GCCACATTGG AGGAGTACAA AGCTACTGTG GCCAGTGACC AGATAGAGAT GAATCGCTTG 
AAGGCCCAGC TGGAGAACGA AAAGCAGAAG GTGGCTGAGT TGTATTCTAT CCATAACTCT 
GGAGACAAGT CCGATATCCA GGATCTCCTG GAGAGTGTCA GGCTGGACAA GGAAAAGGCG 
GAGACTTTGG CCAGTAGCCT GCAGGAAGAT CTGGCTCATA CCCGAAATGA TGCCAATCGG 
TTACAGGACA CCATTGCTAA GGTAGAAGAT GAATACCGAG CTTTCCAGGA AGAGGCCAAG 
AAGCAGATTG AAGATTTGAA TATGACATTA GAAAAACTAA GATCAGAGTT GGAGGAAAAG 
GACACGGAGA GGAGTGACAT GAAGGAAACC ATCTTTGAAC TTGAAGATGA GGTGGAGCAG 
CACCGCGCCG TGAAGCTGCA TGACAACCTC ATTATCTCTG ATCTAGAGAA TACAGTTAAG 
AAACTCCAAG ACCAAAAGCA TGACATGGAA AGAGAAATCA AGACACTTCA CAGGAGACTT 
CGGGAAGAGT CTGCAGAATG GCGGCAATTT CAGGCTGATC TCCAGACTGC AGTGGTCATT 
GCCAATGATA TCAAGTCTGA AGCCCAAGAA GAGATCGGTG ATCTAAAGCG ACGGCTACAT 
GAGGCCCAAG AGAAAAATGA GAAACTCACA AAAGAATTGG AGGAAATAAA GTCACGCAAG 
CAAGAGGAAG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTTGAAAG AGATTTGGCA 
GCCTTGAGGC AAGGGATGGG ACTGAGTAGA AGGTCCTCCA CTTCCTCAGA ACCAACTCCC 
ACAGTAAAAA CCCTCATCAA GTCTTTCGAC AGTGCATCTC AAGTACCAAA CGCTGCTGCA 
GCTGCGATCC CTCGAACACC TCTGAGTCCA AGTCCCATGA AAACCCCTCC TGCAGCTGCC 
GTTTCTCCCA TGCAGAGACA TTCCATAAGT GGTCCAATCT CAACCTCCAA GCCTCTGACA 
GCCTTGTCAG ATAAGAGATC AAACTATGGA GAGCTTCCTG TACAAGAGCA TCTACTAAGA 
ACATCTTCAA CCAGCCGGCC CGCCTCTCTC CCAAGAGTCC CTGCTATGGA AAGTGCCAAG 
ACCATCTCAG TGTCTCGACG GAGTAGTGAA GAAATGAAAC GGGACATCTC TGCATCTGAG 
GGCGCGTCAC CAGCCTCTCT CATGGCTATG GGCACCACGT CACCACAGCT CTCCCTCTCT 
TCCTCTCCCA CTGCATCTGT CACTCCAAGC ACCCGGAGCC GAATAAGGGA AGAAAGGAAA 
GACCCCCTCT CAGCATTGGC AAGAGAATAT GGAGGCTCCA AGAGGAACGC CTTGTTGAAG 
TGGTGTCAGA AGAAAACAGA AGGCTATCAG AATATTGACA TCACAAACTT CAGCAGCAGC 
TGGAACGACG GGCTGGCTTT CTGCGCATTG CTTCATACCT ATCTCCCAGC CCACATACCA 
TATCAAGAAC TGAACAGTCA AGATAAGAAA AGGAACTTCA CCCTGGCTTT CCAGGCAGCT 
GAAAGTGTTG GAATCAAATC GACGCTGGAC ATCAATGAAA TGGCGCGTAC TGAACGGCCC 
GACTGGCAGA ACGTCATGCT GTATGTTACA GCCATCTACA AGTACTTTGA AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Specc1l ( XM_011243589.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241891.1 Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X1, mRNA.

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CloneID	OMu58421
Clone ID Related Accession (Same CDS sequence)	NM_001145826.1 , XM_011243589.2
Accession Version	XM_011243589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytospin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAAC TAAGGTTTGG TTGTAAATGC ATCACCAAGA GGCAGCCCAG AATGAAGAAA 
GCAAACAGGA GTGCTGGCTC AGTGCCTAAG GTGTCTGGGA TAAGCAAACC GCAAACAGTA 
GAGAAAAGCA AGCCTGAGAA CAGCTCTTCA GCACCCACAG GAGTCAAGCC TGTGAGGCCT 
GGGGCAGCAG CAGCCTTGTC AAAGACCAAG AGCAATGATG ACCTTTTAGC TGGAATGGCT 
GGAGGAGTAA ACGTGACTAA TGGTATTAAA GCAAAGAAAA GCACCTGTTC ATCTGCAGCG 
CCTTCAGCCC CCGCCCCTGC CATGACCATC TCGGAGAACA AGTCCAAGAT CAGCACAGGC 
ACTAGTTCCT CAGCCAAACG GAGTACTTCG GCTGGTAATA AAGAGTCCAG TTCTACTAGA 
GAAAGATTAC GTGAACGCAC CCGGCTAAAC CAGAGCAAAA AACTGCCCTC TGTAAGTCAG 
GGAGCTAATG ATGTGGCACT GGCTAAACGT TCACGCAGCC GCACTGCTGC AGAGGGTGAT 
ATTCGTATGA GCAAGTCTAA GTCGGACAAT CAGATCAGTG ACAAAGCTGC TTTGGAAGCC 
AAGGTGAAAG ATCTCCTCAC ACTGGCGAAA ACCAAGGATG TGGAAATTTT ACATTTGAGA 
AATGAACTCC GAGACATGCG TGCTCAGCTG GGCATTAGTG AGGACCATTG TGAAGGTGAG 
GACAGGTCTG AGGTGAAGGA AACTATTATT GCTCACCAGC CTACTGACGT GGAGTCTACT 
CTCCTGCAGC TGCAGGAGCA GAATACAGCC ATCCGCGAGG AGCTCAACCA GCTGAAGAAT 
GAAAACAGAA TGCTAAAGGA CAGGCTCAAT GCTTTGGGCT TTTCCCTAGA GCAAAGGCTG 
GACAATTCTG AAAAACTATT TGGCTATCAG TCCCTGAGCC CAGAAATCAC CCCTGGTAAC 
CAGAGTGACG GAGGAGGAAC TCTGACATCT TCGGTAGAAG GTTCTGCTCC TGGGTCGGTG 
GAAGATCTCC TGAGTCAGGA TGAAAATACA CTCATGGACC ATCAGCACAG TAACTCCATG 
GACAACCTGG ACAGTGAGTG CAGCGAGGTG TACCAGCCCC TCACTTCCAG CGATGATGCT 
CTGGACGCCC CCTCCTCTTC AGAGTCAGAG GGCGTGCCAA GCATTGAGCG CTCTCGGAAG 
GGGAGCAGTG GGAATGCCAG TGAGGTGTCC GTCGCTTGCC TGACAGAACG GATTCACCAG 
ATGGAAGAGA ACCAGCACAG CACCAGTGAG GAGCTTCAGG CCACTCTACA GGAGCTGGCT 
GATCTCCAGC AGATTACCCA GGAGCTCAAC AGTGAGAACG AAAGGCTGGG AGAGGAGAAG 
GTCATTCTGA TGGAGTCGCT GTGTCAGCAG AGTGATAAGT TGGAACACTT TGGCCGGCAG 
ATTGAGTATT TCCGTTCCCT TCTTGATGAG CATCACATTT CCTATGTCAT AGATGAAGAC 
GTAAAGAGCG GGCGCTATAT GGAACTAGAG CAGCGCTACA TGGATTTGGC TGAGAATGCC 
CGTTTTGAGC GAGAGCAGCT TCTAGGTGTG CAGCAGCATT TAAGCAACAC TTTGAAGATG 
GCAGAACAAG ATAACAAAGA AGCTCAAGAA ATGATAGGTG CCCTCAAAGA GCGCAGCCAC 
CACATGGAGC GGATTATTGA GTCTGAGCAG AAGGGCAAAG CGGCCCTGGC AGCCACATTG 
GAGGAGTACA AAGCTACTGT GGCCAGTGAC CAGATAGAGA TGAATCGCTT GAAGGCCCAG 
CTGGAGAACG AAAAGCAGAA GGTGGCTGAG TTGTATTCTA TCCATAACTC TGGAGACAAG 
TCCGATATCC AGGATCTCCT GGAGAGTGTC AGGCTGGACA AGGAAAAGGC GGAGACTTTG 
GCCAGTAGCC TGCAGGAAGA TCTGGCTCAT ACCCGAAATG ATGCCAATCG GTTACAGGAC 
ACCATTGCTA AGGTAGAAGA TGAATACCGA GCTTTCCAGG AAGAGGCCAA GAAGCAGATT 
GAAGATTTGA ATATGACATT AGAAAAACTA AGATCAGAGT TGGAGGAAAA GGACACGGAG 
AGGAGTGACA TGAAGGAAAC CATCTTTGAA CTTGAAGATG AGGTGGAGCA GCACCGCGCC 
GTGAAGCTGC ATGACAACCT CATTATCTCT GATCTAGAGA ATACAGTTAA GAAACTCCAA 
GACCAAAAGC ATGACATGGA AAGAGAAATC AAGACACTTC ACAGGAGACT TCGGGAAGAG 
TCTGCAGAAT GGCGGCAATT TCAGGCTGAT CTCCAGACTG CAGTGGTCAT TGCCAATGAT 
ATCAAGTCTG AAGCCCAAGA AGAGATCGGT GATCTAAAGC GACGGCTACA TGAGGCCCAA 
GAGAAAAATG AGAAACTCAC AAAAGAATTG GAGGAAATAA AGTCACGCAA GCAAGAGGAA 
GAGCGTGGCC GGGTGTATAA CTACATGAAT GCTGTTGAAA GAGATTTGGC AGCCTTGAGG 
CAAGGGATGG GACTGAGTAG AAGGTCCTCC ACTTCCTCAG AACCAACTCC CACAGTAAAA 
ACCCTCATCA AGTCTTTCGA CAGTGCATCT CAAGTACCAA ACGCTGCTGC AGCTGCGATC 
CCTCGAACAC CTCTGAGTCC AAGTCCCATG AAAACCCCTC CTGCAGCTGC CGTTTCTCCC 
ATGCAGAGAC ATTCCATAAG TGGTCCAATC TCAACCTCCA AGCCTCTGAC AGCCTTGTCA 
GATAAGAGAT CAAACTATGG AGAGCTTCCT GTACAAGAGC ATCTACTAAG AACATCTTCA 
ACCAGCCGGC CCGCCTCTCT CCCAAGAGTC CCTGCTATGG AAAGTGCCAA GACCATCTCA 
GTGTCTCGAC GGAGTAGTGA AGAAATGAAA CGGGACATCT CTGCATCTGA GGGCGCGTCA 
CCAGCCTCTC TCATGGCTAT GGGCACCACG TCACCACAGC TCTCCCTCTC TTCCTCTCCC 
ACTGCATCTG TCACTCCAAG CACCCGGAGC CGAATAAGGG AAGAAAGGAA AGACCCCCTC 
TCAGCATTGG CAAGAGAATA TGGAGGCTCC AAGAGGAACG CCTTGTTGAA GTGGTGTCAG 
AAGAAAACAG AAGGCTATCA GAATATTGAC ATCACAAACT TCAGCAGCAG CTGGAACGAC 
GGGCTGGCTT TCTGCGCATT GCTTCATACC TATCTCCCAG CCCACATACC ATATCAAGAA 
CTGAACAGTC AAGATAAGAA AAGGAACTTC ACCCTGGCTT TCCAGGCAGC TGAAAGTGTT 
GGAATCAAAT CGACGCTGGA CATCAATGAA ATGGCGCGTA CTGAACGGCC CGACTGGCAG 
AACGTCATGC TGTATGTTAC AGCCATCTAC AAGTACTTTG AAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241891.1
CDS	618..4025
Translation	MLELRFGCKCITKRQPRMKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	XM_011243589.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243589.2

ORF Insert Sequence:

ATGTTGGAAC TAAGGTTTGG TTGTAAATGC ATCACCAAGA GGCAGCCCAG AATGAAGAAA 
GCAAACAGGA GTGCTGGCTC AGTGCCTAAG GTGTCTGGGA TAAGCAAACC GCAAACAGTA 
GAGAAAAGCA AGCCTGAGAA CAGCTCTTCA GCACCCACAG GAGTCAAGCC TGTGAGGCCT 
GGGGCAGCAG CAGCCTTGTC AAAGACCAAG AGCAATGATG ACCTTTTAGC TGGAATGGCT 
GGAGGAGTAA ACGTGACTAA TGGTATTAAA GCAAAGAAAA GCACCTGTTC ATCTGCAGCG 
CCTTCAGCCC CCGCCCCTGC CATGACCATC TCGGAGAACA AGTCCAAGAT CAGCACAGGC 
ACTAGTTCCT CAGCCAAACG GAGTACTTCG GCTGGTAATA AAGAGTCCAG TTCTACTAGA 
GAAAGATTAC GTGAACGCAC CCGGCTAAAC CAGAGCAAAA AACTGCCCTC TGTAAGTCAG 
GGAGCTAATG ATGTGGCACT GGCTAAACGT TCACGCAGCC GCACTGCTGC AGAGGGTGAT 
ATTCGTATGA GCAAGTCTAA GTCGGACAAT CAGATCAGTG ACAAAGCTGC TTTGGAAGCC 
AAGGTGAAAG ATCTCCTCAC ACTGGCGAAA ACCAAGGATG TGGAAATTTT ACATTTGAGA 
AATGAACTCC GAGACATGCG TGCTCAGCTG GGCATTAGTG AGGACCATTG TGAAGGTGAG 
GACAGGTCTG AGGTGAAGGA AACTATTATT GCTCACCAGC CTACTGACGT GGAGTCTACT 
CTCCTGCAGC TGCAGGAGCA GAATACAGCC ATCCGCGAGG AGCTCAACCA GCTGAAGAAT 
GAAAACAGAA TGCTAAAGGA CAGGCTCAAT GCTTTGGGCT TTTCCCTAGA GCAAAGGCTG 
GACAATTCTG AAAAACTATT TGGCTATCAG TCCCTGAGCC CAGAAATCAC CCCTGGTAAC 
CAGAGTGACG GAGGAGGAAC TCTGACATCT TCGGTAGAAG GTTCTGCTCC TGGGTCGGTG 
GAAGATCTCC TGAGTCAGGA TGAAAATACA CTCATGGACC ATCAGCACAG TAACTCCATG 
GACAACCTGG ACAGTGAGTG CAGCGAGGTG TACCAGCCCC TCACTTCCAG CGATGATGCT 
CTGGACGCCC CCTCCTCTTC AGAGTCAGAG GGCGTGCCAA GCATTGAGCG CTCTCGGAAG 
GGGAGCAGTG GGAATGCCAG TGAGGTGTCC GTCGCTTGCC TGACAGAACG GATTCACCAG 
ATGGAAGAGA ACCAGCACAG CACCAGTGAG GAGCTTCAGG CCACTCTACA GGAGCTGGCT 
GATCTCCAGC AGATTACCCA GGAGCTCAAC AGTGAGAACG AAAGGCTGGG AGAGGAGAAG 
GTCATTCTGA TGGAGTCGCT GTGTCAGCAG AGTGATAAGT TGGAACACTT TGGCCGGCAG 
ATTGAGTATT TCCGTTCCCT TCTTGATGAG CATCACATTT CCTATGTCAT AGATGAAGAC 
GTAAAGAGCG GGCGCTATAT GGAACTAGAG CAGCGCTACA TGGATTTGGC TGAGAATGCC 
CGTTTTGAGC GAGAGCAGCT TCTAGGTGTG CAGCAGCATT TAAGCAACAC TTTGAAGATG 
GCAGAACAAG ATAACAAAGA AGCTCAAGAA ATGATAGGTG CCCTCAAAGA GCGCAGCCAC 
CACATGGAGC GGATTATTGA GTCTGAGCAG AAGGGCAAAG CGGCCCTGGC AGCCACATTG 
GAGGAGTACA AAGCTACTGT GGCCAGTGAC CAGATAGAGA TGAATCGCTT GAAGGCCCAG 
CTGGAGAACG AAAAGCAGAA GGTGGCTGAG TTGTATTCTA TCCATAACTC TGGAGACAAG 
TCCGATATCC AGGATCTCCT GGAGAGTGTC AGGCTGGACA AGGAAAAGGC GGAGACTTTG 
GCCAGTAGCC TGCAGGAAGA TCTGGCTCAT ACCCGAAATG ATGCCAATCG GTTACAGGAC 
ACCATTGCTA AGGTAGAAGA TGAATACCGA GCTTTCCAGG AAGAGGCCAA GAAGCAGATT 
GAAGATTTGA ATATGACATT AGAAAAACTA AGATCAGAGT TGGAGGAAAA GGACACGGAG 
AGGAGTGACA TGAAGGAAAC CATCTTTGAA CTTGAAGATG AGGTGGAGCA GCACCGCGCC 
GTGAAGCTGC ATGACAACCT CATTATCTCT GATCTAGAGA ATACAGTTAA GAAACTCCAA 
GACCAAAAGC ATGACATGGA AAGAGAAATC AAGACACTTC ACAGGAGACT TCGGGAAGAG 
TCTGCAGAAT GGCGGCAATT TCAGGCTGAT CTCCAGACTG CAGTGGTCAT TGCCAATGAT 
ATCAAGTCTG AAGCCCAAGA AGAGATCGGT GATCTAAAGC GACGGCTACA TGAGGCCCAA 
GAGAAAAATG AGAAACTCAC AAAAGAATTG GAGGAAATAA AGTCACGCAA GCAAGAGGAA 
GAGCGTGGCC GGGTGTATAA CTACATGAAT GCTGTTGAAA GAGATTTGGC AGCCTTGAGG 
CAAGGGATGG GACTGAGTAG AAGGTCCTCC ACTTCCTCAG AACCAACTCC CACAGTAAAA 
ACCCTCATCA AGTCTTTCGA CAGTGCATCT CAAGTACCAA ACGCTGCTGC AGCTGCGATC 
CCTCGAACAC CTCTGAGTCC AAGTCCCATG AAAACCCCTC CTGCAGCTGC CGTTTCTCCC 
ATGCAGAGAC ATTCCATAAG TGGTCCAATC TCAACCTCCA AGCCTCTGAC AGCCTTGTCA 
GATAAGAGAT CAAACTATGG AGAGCTTCCT GTACAAGAGC ATCTACTAAG AACATCTTCA 
ACCAGCCGGC CCGCCTCTCT CCCAAGAGTC CCTGCTATGG AAAGTGCCAA GACCATCTCA 
GTGTCTCGAC GGAGTAGTGA AGAAATGAAA CGGGACATCT CTGCATCTGA GGGCGCGTCA 
CCAGCCTCTC TCATGGCTAT GGGCACCACG TCACCACAGC TCTCCCTCTC TTCCTCTCCC 
ACTGCATCTG TCACTCCAAG CACCCGGAGC CGAATAAGGG AAGAAAGGAA AGACCCCCTC 
TCAGCATTGG CAAGAGAATA TGGAGGCTCC AAGAGGAACG CCTTGTTGAA GTGGTGTCAG 
AAGAAAACAG AAGGCTATCA GAATATTGAC ATCACAAACT TCAGCAGCAG CTGGAACGAC 
GGGCTGGCTT TCTGCGCATT GCTTCATACC TATCTCCCAG CCCACATACC ATATCAAGAA 
CTGAACAGTC AAGATAAGAA AAGGAACTTC ACCCTGGCTT TCCAGGCAGC TGAAAGTGTT 
GGAATCAAAT CGACGCTGGA CATCAATGAA ATGGCGCGTA CTGAACGGCC CGACTGGCAG 
AACGTCATGC TGTATGTTAC AGCCATCTAC AAGTACTTTG AAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.
Scientific reports617735(2016 Jan)
Wilson NR,Olm-Shipman AJ,Acevedo DS,Palaniyandi K,Hall EG,Kosa E,Stumpff KM,Smith GJ,Pitstick L,Liao EC,Bjork BC,Czirok A,Saadi I

Deficiency of the cytoskeletal protein SPECC1L leads to oblique facial clefting.
American journal of human genetics89(1)44-55(2011 Jul)
Saadi I,Alkuraya FS,Gisselbrecht SS,Goessling W,Cavallesco R,Turbe-Doan A,Petrin AL,Harris J,Siddiqui U,Grix AW Jr,Hove HD,Leboulch P,Glover TW,Morton CC,Richieri-Costa A,Murray JC,Erickson RP,Maas RL

A mutation in the pericentrin gene causes abnormal interneuron migration to the olfactory bulb in mice.
Developmental biology340(1)41-53(2010 Apr)
Endoh-Yamagami S,Karkar KM,May SR,Cobos I,Thwin MT,Long JE,Ashique AM,Zarbalis K,Rubenstein JL,Peterson AS

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes9(5)179-88(2002 Oct)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Hara Y,Nagase T,Ohara O,Koga H

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Specc1l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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