Bclaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bclaf1
Entrez Gene ID	72567
Full Name	BCL2-associated transcription factor 1
Synonyms	2610102K23Rik,2700025J07Rik,2810454G14Rik,5730534O06Rik,AI450190,AW556225,Btf,mKIAA0164
General protein information	Preferred Names BCL2-associated transcription factor 1 Names bcl-2-associated transcription factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512865.3	XP_006512928.1	bcl-2-associated transcription factor 1 isoform X1
XM_011243207.2	XP_011241509.1	bcl-2-associated transcription factor 1 isoform X2
XM_011243207.3	XP_011241509.1	bcl-2-associated transcription factor 1 isoform X2
XM_006512866.3	XP_006512929.1	bcl-2-associated transcription factor 1 isoform X3
XM_006512865.4	XP_006512928.1	bcl-2-associated transcription factor 1 isoform X1
NM_001025393.1	NP_001020564.1	bcl-2-associated transcription factor 1 isoform 3
NM_001025392.1	NP_001020563.1	bcl-2-associated transcription factor 1 isoform 1
NM_153787.2	NP_722482.1	bcl-2-associated transcription factor 1 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BCLAF1	XP_860336.1
Pan troglodytes (chimpanzee)	BCLAF1	XP_518759.3
Rattus norvegicus (Norway rat)	Bclaf1	NP_001041317.2
Xenopus tropicalis (tropical clawed frog)	bclaf1	NP_001116963.1
Homo sapiens (human)	BCLAF1	NP_055554.1
Gallus gallus (chicken)	BCLAF1	XP_003640970.2
Bos taurus (cattle)	BCLAF1	NP_001091586.1
Mus musculus (house mouse)	Bclaf1	NP_001020563.1

Gene Ontology
Bibliography

Related articles in PubMed

Constitutive expression of the anti-apoptotic Bcl-2 family member A1 in murine endothelial cells leads to transplant tolerance.
Smyth LA, Meader L, Xiao F, Woodward M, Brady HJ, Lechler R, Lombardi G
Clinical and experimental immunology188(2)219-225(2017 May)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing.
Wei N, Cheng Y, Wang Z, Liu Y, Luo C, Liu L, Chen L, Xie Z, Lu Y, Feng Y
Cell reports13(8)1647-57(2015 Nov)

Involvement of Bcl-2-associated transcription factor 1 in the differentiation of early-born retinal cells.
Orieux G, Picault L, Slembrouck A, Roger JE, Guillonneau X, Sahel JA, Saule S, McPherson JP, Goureau O
The Journal of neuroscience : the official journal of the Society for Neuroscience34(4)1530-41(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Constitutive expression of the anti-apoptotic molecule Bfl1 (A1) in murine vascular Endothelial Cells leads to prolonged allograft survival due to modifying inflammation.
Title: Constitutive expression of the anti-apoptotic Bcl-2 family member A1 in murine endothelial cells leads to transplant tolerance.

Bclaf1 facilitates the differentiation of early retinal precursors into retinal ganglion cells, amacrine cells, and horizontal cells rather than into cone photoreceptors.
Title: Involvement of Bcl-2-associated transcription factor 1 in the differentiation of early-born retinal cells.

Sirt1 negatively regulates T cell activation via H3K56 deacetylation at the promoter region to inhibit transcription of Bclaf1
Title: The type III histone deacetylase Sirt1 protein suppresses p300-mediated histone H3 lysine 56 acetylation at Bclaf1 promoter to inhibit T cell activation.

Bclaf1 expression was found to be strongly upregulated during the saccular stage of murine embryonic lung development and was dispensable for thymocyte development.
Title: Essential role for Bclaf1 in lung development and immune system function.

The following Bclaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bclaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43967	XM_006512866.3 Latest version!	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu14218	NM_001025393.1 Latest version!	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15557	NM_001025392.1 Latest version!	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
OMu15739	NM_153787.2 Latest version!	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$538.30 $769.00
OMu43966	XM_006512865.4 Latest version!	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu70924	XM_011243207.3 Latest version!	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu70924	XM_011243207.2	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OMu43966	XM_006512865.3	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bclaf1 ( XM_006512866.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512929.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu43967
Clone ID Related Accession (Same CDS sequence)	XM_006512866.3
Accession Version	XM_006512866.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA TACTGGGCGA ACAATACTTC AAATCACCTG CAGTGACCCT AAACGAGCGG 
TTCACTTCAT ACCAGAAAGC TACTGAAGAA CATAGTACCC GGCAAAAGAG CCCCGAGATA 
CACAGGAGGA TTGACATCTC TCCCAGCGCT CTGAGGAAGC ACACCCGGCT AGCAGGAGAG 
GAGAGAGGTT TTAAAGAAGA AATTCAGAAG GGAGATAAAA AGTTAAGGTG TGATTCGGCT 
GACCTTCGGC ACGACATTGA CCGTCGCCGT AAAGAGAGAA GTAAAGAGCG GGGAGATTCC 
AAGGGCTCCA GGGAGTCCAG TGGGTCCAGA AAGCAAGAAA AAACACCAAA GGATTACAAG 
GAGTATAAAC CTTACAAAGA TGACAGCAAA CATAAAGGTA GAGAGCGGGA CCATTCCCGG 
TCATCGTCCT CGTCAGCATC GCCGTCCTCG CCCAGTTCTC GAGAAGAAAA AGAGAGTAAG 
AAAGAGAGAG AAGAAGAATT TAAAACTCAC CACGAGATGA AAGACTACTC AGGATTTGCA 
GGAGTTAGCA GACCACGAGG AACCTTTTTT CGAATTAGAG GCAGAGGAAG AGCCAGAGGA 
GTTTTTGCTG GGACAAATAC TGGTCCAAAC AACTCAAATA CCACTTTTCA AAAGAGACCG 
AAGGAAGAGG AATGGGATCC AGAATATACC CCAAAGAGCA AGAAGTACTT CTTGCACGAT 
GACAGAGATG ATGGTGTGGA TTACTGGGCC AAAAGAGGAA GAGGTCGTGG TACTTTTCAA 
CGTGGCAGAG GGCGCTTTAA TTTCAAAAAA TCAGGTAGCA GTCCAAAATG GACTCATGAC 
AAATACCAAG GAGATGGGAT TGTTGAAGAT GACGAAGAGA CCATGGAAAA TAATGAAGAG 
AAGAAGGACA GACGCAAAGA AGAAAAGGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512929.1
CDS	265..1197
Translation	MCWILGEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKDYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKDRRKEEKE

Target ORF information:

RefSeq Version	XM_006512866.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512866.3

ORF Insert Sequence:

ATGTGCTGGA TACTGGGCGA ACAATACTTC AAATCACCTG CAGTGACCCT AAACGAGCGG 
TTCACTTCAT ACCAGAAAGC TACTGAAGAA CATAGTACCC GGCAAAAGAG CCCCGAGATA 
CACAGGAGGA TTGACATCTC TCCCAGCGCT CTGAGGAAGC ACACCCGGCT AGCAGGAGAG 
GAGAGAGGTT TTAAAGAAGA AATTCAGAAG GGAGATAAAA AGTTAAGGTG TGATTCGGCT 
GACCTTCGGC ACGACATTGA CCGTCGCCGT AAAGAGAGAA GTAAAGAGCG GGGAGATTCC 
AAGGGCTCCA GGGAGTCCAG TGGGTCCAGA AAGCAAGAAA AAACACCAAA GGATTACAAG 
GAGTATAAAC CTTACAAAGA TGACAGCAAA CATAAAGGTA GAGAGCGGGA CCATTCCCGG 
TCATCGTCCT CGTCAGCATC GCCGTCCTCG CCCAGTTCTC GAGAAGAAAA AGAGAGTAAG 
AAAGAGAGAG AAGAAGAATT TAAAACTCAC CACGAGATGA AAGACTACTC AGGATTTGCA 
GGAGTTAGCA GACCACGAGG AACCTTTTTT CGAATTAGAG GCAGAGGAAG AGCCAGAGGA 
GTTTTTGCTG GGACAAATAC TGGTCCAAAC AACTCAAATA CCACTTTTCA AAAGAGACCG 
AAGGAAGAGG AATGGGATCC AGAATATACC CCAAAGAGCA AGAAGTACTT CTTGCACGAT 
GACAGAGATG ATGGTGTGGA TTACTGGGCC AAAAGAGGAA GAGGTCGTGG TACTTTTCAA 
CGTGGCAGAG GGCGCTTTAA TTTCAAAAAA TCAGGTAGCA GTCCAAAATG GACTCATGAC 
AAATACCAAG GAGATGGGAT TGTTGAAGAT GACGAAGAGA CCATGGAAAA TAATGAAGAG 
AAGAAGGACA GACGCAAAGA AGAAAAGGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( XM_011243207.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241509.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu70924
Clone ID Related Accession (Same CDS sequence)	XM_011243207.2 , XM_011243207.3
Accession Version	XM_011243207.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAAAAT 
GTATTTGTCT TTTACACGGC TTATGGTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241509.1
CDS	642..2894
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKNVFVFYTAYGG

Target ORF information:

RefSeq Version	XM_011243207.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243207.2

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAAAAT 
GTATTTGTCT TTTACACGGC TTATGGTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( NM_001025393.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001020564.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu14218
Clone ID Related Accession (Same CDS sequence)	NM_001025393.1
Accession Version	NM_001025393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY071148.1, BC086624.1, CK637794.1, CN530237.1 and CAAA01031508.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, as compared to variant 1. The encoded isoform 3 lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086624.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAACAT 
AAAGGTAGAG AGCGGGACCA TTCCCGGTCA TCGTCCTCGT CAGCATCGCC GTCCTCGCCC 
AGTTCTCGAG AAGAAAAAGA GAGTAAGAAA GAGAGAGAAG AAGAATTTAA AACTCACCAC 
GAGATGAAAG ACTACTCAGG ATTTGCAGGA GTTAGCAGAC CACGAGGAAC CTTTCACGAT 
GACAGAGATG ATGGTGTGGA TTACTGGGCC AAAAGAGGAA GAGGTCGTGG TACTTTTCAA 
CGTGGCAGAG GGCGCTTTAA TTTCAAAAAA TCAGGTAGCA GTCCAAAATG GACTCATGAC 
AAATACCAAG GAGATGGGAT TGTTGAAGAT GACGAAGAGA CCATGGAAAA TAATGAAGAG 
AAGAAGGACA GACGCAAAGA AGAAAAGGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020564.1
CDS	251..2863
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKDYSGFAGVSRPRGTFHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKDRRKEEKE

Target ORF information:

RefSeq Version	NM_001025393.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025393.1

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAACAT 
AAAGGTAGAG AGCGGGACCA TTCCCGGTCA TCGTCCTCGT CAGCATCGCC GTCCTCGCCC 
AGTTCTCGAG AAGAAAAAGA GAGTAAGAAA GAGAGAGAAG AAGAATTTAA AACTCACCAC 
GAGATGAAAG ACTACTCAGG ATTTGCAGGA GTTAGCAGAC CACGAGGAAC CTTTCACGAT 
GACAGAGATG ATGGTGTGGA TTACTGGGCC AAAAGAGGAA GAGGTCGTGG TACTTTTCAA 
CGTGGCAGAG GGCGCTTTAA TTTCAAAAAA TCAGGTAGCA GTCCAAAATG GACTCATGAC 
AAATACCAAG GAGATGGGAT TGTTGAAGAT GACGAAGAGA CCATGGAAAA TAATGAAGAG 
AAGAAGGACA GACGCAAAGA AGAAAAGGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( NM_001025392.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001020563.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 1, mRNA.

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CloneID	OMu15557
Clone ID Related Accession (Same CDS sequence)	NM_001025392.1
Accession Version	NM_001025392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY071148.1, BC029682.1, BC034300.1 and CAAA01031508.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.283037.1, BC029682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAACAT 
AAAGGTAGAG AGCGGGACCA TTCCCGGTCA TCGTCCTCGT CAGCATCGCC GTCCTCGCCC 
AGTTCTCGAG AAGAAAAAGA GAGTAAGAAA GAGAGAGAAG AAGAATTTAA AACTCACCAC 
GAGATGAAAG ACTACTCAGG ATTTGCAGGA GTTAGCAGAC CACGAGGAAC CTTTTTTCGA 
ATTAGAGGCA GAGGAAGAGC CAGAGGAGTT TTTGCTGGGA CAAATACTGG TCCAAACAAC 
TCAAATACCA CTTTTCAAAA GAGACCGAAG GAAGAGGAAT GGGATCCAGA ATATACCCCA 
AAGAGCAAGA AGTACTTCTT GCACGATGAC AGAGATGATG GTGTGGATTA CTGGGCCAAA 
AGAGGAAGAG GTCGTGGTAC TTTTCAACGT GGCAGAGGGC GCTTTAATTT CAAAAAATCA 
GGTAGCAGTC CAAAATGGAC TCATGACAAA TACCAAGGAG ATGGGATTGT TGAAGATGAC 
GAAGAGACCA TGGAAAATAA TGAAGAGAAG AAGGACAGAC GCAAAGAAGA AAAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020563.1
CDS	251..3010
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKDYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKDRRKEEKE

Target ORF information:

RefSeq Version	NM_001025392.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025392.1

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAACAT 
AAAGGTAGAG AGCGGGACCA TTCCCGGTCA TCGTCCTCGT CAGCATCGCC GTCCTCGCCC 
AGTTCTCGAG AAGAAAAAGA GAGTAAGAAA GAGAGAGAAG AAGAATTTAA AACTCACCAC 
GAGATGAAAG ACTACTCAGG ATTTGCAGGA GTTAGCAGAC CACGAGGAAC CTTTTTTCGA 
ATTAGAGGCA GAGGAAGAGC CAGAGGAGTT TTTGCTGGGA CAAATACTGG TCCAAACAAC 
TCAAATACCA CTTTTCAAAA GAGACCGAAG GAAGAGGAAT GGGATCCAGA ATATACCCCA 
AAGAGCAAGA AGTACTTCTT GCACGATGAC AGAGATGATG GTGTGGATTA CTGGGCCAAA 
AGAGGAAGAG GTCGTGGTAC TTTTCAACGT GGCAGAGGGC GCTTTAATTT CAAAAAATCA 
GGTAGCAGTC CAAAATGGAC TCATGACAAA TACCAAGGAG ATGGGATTGT TGAAGATGAC 
GAAGAGACCA TGGAAAATAA TGAAGAGAAG AAGGACAGAC GCAAAGAAGA AAAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( XM_006512865.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512928.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43966
Clone ID Related Accession (Same CDS sequence)	XM_006512865.3 , XM_006512865.4
Accession Version	XM_006512865.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGGTCTCG TTCCAGAACA 
TATTCGAGGT CTCGTAGTAG AGATCGTATT TATTCTAGAG ATTATCGTCG AGATTACAGG 
AATAATAGAG GAATGAGACG ACCTTATGGG TACAGAGGAA GGGGTAGAGG GTATTATCAA 
GGAGGAGGAG GGAGATACCA TCGAGGTGGC TATAGACCTG TCTGGAATAG AAGGCACTCT 
AGGAGTCCTA GACGAGGTCG GTCACGGTCC AGGAGTCCAA AAAGAAGATC CGTGTCTTCT 
CAAAGATCCC GAAGCAGATC TCGCCGGTCA TATAGATCCT CTAGGTCTCC AAGATCATCA 
TCATCTCGCT CTTCATCCCC ATATAGCAAA TCTCCTGTCT CTAAAAGACG AGGGTCTCAG 
GAAAAACAAA CCAAAAAAGC TGAGGGGGAA CCCCAAGAAG AGAGTCCTTT GAAAAGCAAA 
TCACAGGAGG AACCAAAGGA TACTTTTGAA CATGATCCAT CTGAATCTAT TGATGAGTTT 
AATAAATCTG CTACTTCTGG TGATATTTGG CCTGGCCTTT CAGCTTATGA TAATAGTCCA 
AGGTCACCTC ATAGTCCTTC ACCTATTGCT ACACCACCCA GTCAGAGTTC ATCATGCTCA 
GATGCCCCCA TGCTTAGTAC AGTGCACTCT GCCAAAAATA CCCCTTCTCA GCATTCACAT 
TCCATTCAGC ACAGTCCTGA GAGGTCTGGA TCTGGCTCTG TTGGAAATGG GTCGAGTCGA 
TATAGTCCTT CTCAGAATAG TCCGATTCAT CACATCCCAT CACGAAGAAG CCCTGCCAAG 
ACAATCACAC CACAGAATGC TCCAAGAGAG GAATCTAGGG GGCGGTCCTC CTTTTATCCT 
GAAGGAGATC AGGAAACAGC AAAGACAGGA AAGTTTTTAA AAAGGTTCAC AGATGAAGAG 
TCTAGAGTAT TCCTGCTTGA TAGGGGTAAT ATCAGGGATA AAGAGGCTCC GAAGGAGAAA 
GGGTCAGAGA AAGGCAGGGC AGATGGAGAT TGGGATGACC AGGAAGTTCT AGATTACTTT 
AGTGATAAAG AGTCTGCAAA ACAAAAATTC CATGACTCTG AAGGGGATGA CACAGAGGAG 
ACAGAGGATT ATAGACAGTT TAGGAAATCA GTCCTAGCAG ATCAGGGCAA GAGCTTTGCT 
ACTTCATCTC ACCGGAATAC TGAGGAGGAA GGACCCAAGT ACAAGTCCAA AGTTTCATTA 
AAAGGCAATA GAGAAAGTGA TGGATTTAGA GAAGAAAAAA ATTATAAACT GAAAGAGACT 
GCATACATAG TCGAAAGGCC TAGCACTGCA AAGGACAAGC ACAAGGAAGA AGACAAAGGC 
TCTGATAGAA TAACAGTGAA GAAAGAAGTG CAGTCACCGG AGCAGGTAAA GTCTGAAAAG 
CTCAAAGAGC TCTTTGATTA CAGTCCCCCT CTACACAAGA GTCTGGATGC ACGAGAAAAG 
TCCATCTTCA GAGAGGAGAG CCCACTGAGG ATCAAAATGA TAGCCAGTGA TTCTCACCGT 
CCTGAAGTCA AACTCAAAAT GGCACCTGTT CCTCTTGACG ATTCCAACAG ACCTGCCTCC 
TTGACTAAAG ACAGGCTACT TGCTAGCACG CTTGTCCATT CTGTCAAGAA AGAGCAAGAA 
TTCCGATCCA TCTTTGACCA CATTAAGCTG CCTCAGGCCA ACAAAAGCAC TTCAGAATCA 
TTTATACAAC ACATTGTATC CTTGGTTCAT CATGTGAAAG AACAATACTT CAAATCACCT 
GCAGTGACCC TAAACGAGCG GTTCACTTCA TACCAGAAAG CTACTGAAGA ACATAGTACC 
CGGCAAAAGA GCCCCGAGAT ACACAGGAGG ATTGACATCT CTCCCAGCGC TCTGAGGAAG 
CACACCCGGC TAGCAGGAGA GGAGAGAGGT TTTAAAGAAG AAATTCAGAA GGGAGATAAA 
AAGTTAAGGT GTGATTCGGC TGACCTTCGG CACGACATTG ACCGTCGCCG TAAAGAGAGA 
AGTAAAGAGC GGGGAGATTC CAAGGGCTCC AGGGAGTCCA GTGGGTCCAG AAAGCAAGAA 
AAAACACCAA AGGATTACAA GGAGTATAAA CCTTACAAAG ATGACAGCAA ACATAAAGGT 
AGAGAGCGGG ACCATTCCCG GTCATCGTCC TCGTCAGCAT CGCCGTCCTC GCCCAGTTCT 
CGAGAAGAAA AAGAGAGTAA GAAAGAGAGA GAAGAAGAAT TTAAAACTCA CCACGAGATG 
AAAGACTACT CAGGATTTGC AGGAGTTAGC AGACCACGAG GAACCTTTCA CGATGACAGA 
GATGATGGTG TGGATTACTG GGCCAAAAGA GGAAGAGGTC GTGGTACTTT TCAACGTGGC 
AGAGGGCGCT TTAATTTCAA AAAATCAGGT AGCAGTCCAA AATGGACTCA TGACAAATAC 
CAAGGAGATG GGATTGTTGA AGATGACGAA GAGACCATGG AAAATAATGA AGAGAAGAAG 
GACAGACGCA AAGAAGAAAA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512928.1
CDS	642..3248
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKDYSGFAGVSRPRGTFHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKDRRKEEKE

Target ORF information:

RefSeq Version	XM_006512865.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512865.3

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGGTCTCG TTCCAGAACA 
TATTCGAGGT CTCGTAGTAG AGATCGTATT TATTCTAGAG ATTATCGTCG AGATTACAGG 
AATAATAGAG GAATGAGACG ACCTTATGGG TACAGAGGAA GGGGTAGAGG GTATTATCAA 
GGAGGAGGAG GGAGATACCA TCGAGGTGGC TATAGACCTG TCTGGAATAG AAGGCACTCT 
AGGAGTCCTA GACGAGGTCG GTCACGGTCC AGGAGTCCAA AAAGAAGATC CGTGTCTTCT 
CAAAGATCCC GAAGCAGATC TCGCCGGTCA TATAGATCCT CTAGGTCTCC AAGATCATCA 
TCATCTCGCT CTTCATCCCC ATATAGCAAA TCTCCTGTCT CTAAAAGACG AGGGTCTCAG 
GAAAAACAAA CCAAAAAAGC TGAGGGGGAA CCCCAAGAAG AGAGTCCTTT GAAAAGCAAA 
TCACAGGAGG AACCAAAGGA TACTTTTGAA CATGATCCAT CTGAATCTAT TGATGAGTTT 
AATAAATCTG CTACTTCTGG TGATATTTGG CCTGGCCTTT CAGCTTATGA TAATAGTCCA 
AGGTCACCTC ATAGTCCTTC ACCTATTGCT ACACCACCCA GTCAGAGTTC ATCATGCTCA 
GATGCCCCCA TGCTTAGTAC AGTGCACTCT GCCAAAAATA CCCCTTCTCA GCATTCACAT 
TCCATTCAGC ACAGTCCTGA GAGGTCTGGA TCTGGCTCTG TTGGAAATGG GTCGAGTCGA 
TATAGTCCTT CTCAGAATAG TCCGATTCAT CACATCCCAT CACGAAGAAG CCCTGCCAAG 
ACAATCACAC CACAGAATGC TCCAAGAGAG GAATCTAGGG GGCGGTCCTC CTTTTATCCT 
GAAGGAGATC AGGAAACAGC AAAGACAGGA AAGTTTTTAA AAAGGTTCAC AGATGAAGAG 
TCTAGAGTAT TCCTGCTTGA TAGGGGTAAT ATCAGGGATA AAGAGGCTCC GAAGGAGAAA 
GGGTCAGAGA AAGGCAGGGC AGATGGAGAT TGGGATGACC AGGAAGTTCT AGATTACTTT 
AGTGATAAAG AGTCTGCAAA ACAAAAATTC CATGACTCTG AAGGGGATGA CACAGAGGAG 
ACAGAGGATT ATAGACAGTT TAGGAAATCA GTCCTAGCAG ATCAGGGCAA GAGCTTTGCT 
ACTTCATCTC ACCGGAATAC TGAGGAGGAA GGACCCAAGT ACAAGTCCAA AGTTTCATTA 
AAAGGCAATA GAGAAAGTGA TGGATTTAGA GAAGAAAAAA ATTATAAACT GAAAGAGACT 
GCATACATAG TCGAAAGGCC TAGCACTGCA AAGGACAAGC ACAAGGAAGA AGACAAAGGC 
TCTGATAGAA TAACAGTGAA GAAAGAAGTG CAGTCACCGG AGCAGGTAAA GTCTGAAAAG 
CTCAAAGAGC TCTTTGATTA CAGTCCCCCT CTACACAAGA GTCTGGATGC ACGAGAAAAG 
TCCATCTTCA GAGAGGAGAG CCCACTGAGG ATCAAAATGA TAGCCAGTGA TTCTCACCGT 
CCTGAAGTCA AACTCAAAAT GGCACCTGTT CCTCTTGACG ATTCCAACAG ACCTGCCTCC 
TTGACTAAAG ACAGGCTACT TGCTAGCACG CTTGTCCATT CTGTCAAGAA AGAGCAAGAA 
TTCCGATCCA TCTTTGACCA CATTAAGCTG CCTCAGGCCA ACAAAAGCAC TTCAGAATCA 
TTTATACAAC ACATTGTATC CTTGGTTCAT CATGTGAAAG AACAATACTT CAAATCACCT 
GCAGTGACCC TAAACGAGCG GTTCACTTCA TACCAGAAAG CTACTGAAGA ACATAGTACC 
CGGCAAAAGA GCCCCGAGAT ACACAGGAGG ATTGACATCT CTCCCAGCGC TCTGAGGAAG 
CACACCCGGC TAGCAGGAGA GGAGAGAGGT TTTAAAGAAG AAATTCAGAA GGGAGATAAA 
AAGTTAAGGT GTGATTCGGC TGACCTTCGG CACGACATTG ACCGTCGCCG TAAAGAGAGA 
AGTAAAGAGC GGGGAGATTC CAAGGGCTCC AGGGAGTCCA GTGGGTCCAG AAAGCAAGAA 
AAAACACCAA AGGATTACAA GGAGTATAAA CCTTACAAAG ATGACAGCAA ACATAAAGGT 
AGAGAGCGGG ACCATTCCCG GTCATCGTCC TCGTCAGCAT CGCCGTCCTC GCCCAGTTCT 
CGAGAAGAAA AAGAGAGTAA GAAAGAGAGA GAAGAAGAAT TTAAAACTCA CCACGAGATG 
AAAGACTACT CAGGATTTGC AGGAGTTAGC AGACCACGAG GAACCTTTCA CGATGACAGA 
GATGATGGTG TGGATTACTG GGCCAAAAGA GGAAGAGGTC GTGGTACTTT TCAACGTGGC 
AGAGGGCGCT TTAATTTCAA AAAATCAGGT AGCAGTCCAA AATGGACTCA TGACAAATAC 
CAAGGAGATG GGATTGTTGA AGATGACGAA GAGACCATGG AAAATAATGA AGAGAAGAAG 
GACAGACGCA AAGAAGAAAA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( NM_153787.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_722482.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 2, mRNA.

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CloneID	OMu15739
Clone ID Related Accession (Same CDS sequence)	NM_153787.2
Accession Version	NM_153787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY071148.1, BC034300.1 and CAAA01031508.1. On or before May 4, 2006 this sequence version replaced XM_001052039.1, XM_001052040.1, XM_001052041.1, NM_153787.1. Transcript Variant: This variant (2) lacks a 6-nt segment in the CDS, as compared to variant 1. The encoded isoform 2 lacks 2 internal aa, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.159703.1, BC034300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGGTCTCG TTCCAGAACA 
TATTCGAGGT CTCGTAGTAG AGATCGTATT TATTCTAGAG ATTATCGTCG AGATTACAGG 
AATAATAGAG GAATGAGACG ACCTTATGGG TACAGAGGAA GGGGTAGAGG GTATTATCAA 
GGAGGAGGAG GGAGATACCA TCGAGGTGGC TATAGACCTG TCTGGAATAG AAGGCACTCT 
AGGAGTCCTA GACGAGGTCG GTCACGGTCC AGGAGTCCAA AAAGAAGATC CGTGTCTTCT 
CAAAGATCCC GAAGCAGATC TCGCCGGTCA TATAGATCCT CTAGGTCTCC AAGATCATCA 
TCATCTCGCT CTTCATCCCC ATATAGCAAA TCTCCTGTCT CTAAAAGACG AGGGTCTCAG 
GAAAAACAAA CCAAAAAAGC TGAGGGGGAA CCCCAAGAAG AGAGTCCTTT GAAAAGCAAA 
TCACAGGAGG AACCAAAGGA TACTTTTGAA CATGATCCAT CTGAATCTAT TGATGAGTTT 
AATAAATCTG CTACTTCTGG TGATATTTGG CCTGGCCTTT CAGCTTATGA TAATAGTCCA 
AGGTCACCTC ATAGTCCTTC ACCTATTGCT ACACCACCCA GTCAGAGTTC ATCATGCTCA 
GATGCCCCCA TGCTTAGTAC AGTGCACTCT GCCAAAAATA CCCCTTCTCA GCATTCACAT 
TCCATTCAGC ACAGTCCTGA GAGGTCTGGA TCTGGCTCTG TTGGAAATGG GTCGAGTCGA 
TATAGTCCTT CTCAGAATAG TCCGATTCAT CACATCCCAT CACGAAGAAG CCCTGCCAAG 
ACAATCACAC CACAGAATGC TCCAAGAGAG GAATCTAGGG GGCGGTCCTC CTTTTATCCT 
GAAGGAGATC AGGAAACAGC AAAGACAGGA AAGTTTTTAA AAAGGTTCAC AGATGAAGAG 
TCTAGAGTAT TCCTGCTTGA TAGGGGTAAT ATCAGGGATA AAGAGGCTCC GAAGGAGAAA 
GGGTCAGAGA AAGGCAGGGC AGATGGAGAT TGGGATGACC AGGAAGTTCT AGATTACTTT 
AGTGATAAAG AGTCTGCAAA ACAAAAATTC CATGACTCTG AAGGGGATGA CACAGAGGAG 
ACAGAGGATT ATAGACAGTT TAGGAAATCA GTCCTAGCAG ATCAGGGCAA GAGCTTTGCT 
ACTTCATCTC ACCGGAATAC TGAGGAGGAA GGACCCAAGT ACAAGTCCAA AGTTTCATTA 
AAAGGCAATA GAGAAAGTGA TGGATTTAGA GAAGAAAAAA ATTATAAACT GAAAGAGACT 
GCATACATAG TCGAAAGGCC TAGCACTGCA AAGGACAAGC ACAAGGAAGA AGACAAAGGC 
TCTGATAGAA TAACAGTGAA GAAAGAAGTG CAGTCACCGG AGCAGGTAAA GTCTGAAAAG 
CTCAAAGAGC TCTTTGATTA CAGTCCCCCT CTACACAAGA GTCTGGATGC ACGAGAAAAG 
TCCATCTTCA GAGAGGAGAG CCCACTGAGG ATCAAAATGA TAGCCAGTGA TTCTCACCGT 
CCTGAAGTCA AACTCAAAAT GGCACCTGTT CCTCTTGACG ATTCCAACAG ACCTGCCTCC 
TTGACTAAAG ACAGGCTACT TGCTAGCACG CTTGTCCATT CTGTCAAGAA AGAGCAAGAA 
TTCCGATCCA TCTTTGACCA CATTAAGCTG CCTCAGGCCA ACAAAAGCAC TTCAGAATCA 
TTTATACAAC ACATTGTATC CTTGGTTCAT CATGTGAAAG AACAATACTT CAAATCACCT 
GCAGTGACCC TAAACGAGCG GTTCACTTCA TACCAGAAAG CTACTGAAGA ACATAGTACC 
CGGCAAAAGA GCCCCGAGAT ACACAGGAGG ATTGACATCT CTCCCAGCGC TCTGAGGAAG 
CACACCCGGC TAGCAGGAGA GGAGAGAGGT TTTAAAGAAG AAATTCAGAA GGGAGATAAA 
AAGTTAAGGT GTGATTCGGC TGACCTTCGG CACGACATTG ACCGTCGCCG TAAAGAGAGA 
AGTAAAGAGC GGGGAGATTC CAAGGGCTCC AGGGAGTCCA GTGGGTCCAG AAAGCAAGAA 
AAAACACCAA AGGATTACAA GGAGTATAAA CCTTACAAAG ATGACAGCAA ACATAAAGGT 
AGAGAGCGGG ACCATTCCCG GTCATCGTCC TCGTCAGCAT CGCCGTCCTC GCCCAGTTCT 
CGAGAAGAAA AAGAGAGTAA GAAAGAGAGA GAAGAAGAAT TTAAAACTCA CCACGAGATG 
AAAGACTACT CAGGATTTGC AGGAGTTAGC AGACCACGAG GAACCTTTTT TCGAATTAGA 
GGCAGAGGAA GAGCCAGAGG AGTTTTTGCT GGGACAAATA CTGGTCCAAA CAACTCAAAT 
ACCACTTTTC AAAAGAGACC GAAGGAAGAG GAATGGGATC CAGAATATAC CCCAAAGAGC 
AAGAAGTACT TCTTGCACGA TGACAGAGAT GATGGTGTGG ATTACTGGGC CAAAAGAGGA 
AGAGGTCGTG GTACTTTTCA ACGTGGCAGA GGGCGCTTTA ATTTCAAAAA ATCAGGTAGC 
AGTCCAAAAT GGACTCATGA CAAATACCAA GGAGATGGGA TTGTTGAAGA TGACGAAGAG 
ACCATGGAAA ATAATGAAGA GAAGAAGGAC AGACGCAAAG AAGAAAAGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722482.1
CDS	251..3004
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKDYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKDRRKEEKE

Target ORF information:

RefSeq Version	NM_153787.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153787.2

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGGTCTCG TTCCAGAACA 
TATTCGAGGT CTCGTAGTAG AGATCGTATT TATTCTAGAG ATTATCGTCG AGATTACAGG 
AATAATAGAG GAATGAGACG ACCTTATGGG TACAGAGGAA GGGGTAGAGG GTATTATCAA 
GGAGGAGGAG GGAGATACCA TCGAGGTGGC TATAGACCTG TCTGGAATAG AAGGCACTCT 
AGGAGTCCTA GACGAGGTCG GTCACGGTCC AGGAGTCCAA AAAGAAGATC CGTGTCTTCT 
CAAAGATCCC GAAGCAGATC TCGCCGGTCA TATAGATCCT CTAGGTCTCC AAGATCATCA 
TCATCTCGCT CTTCATCCCC ATATAGCAAA TCTCCTGTCT CTAAAAGACG AGGGTCTCAG 
GAAAAACAAA CCAAAAAAGC TGAGGGGGAA CCCCAAGAAG AGAGTCCTTT GAAAAGCAAA 
TCACAGGAGG AACCAAAGGA TACTTTTGAA CATGATCCAT CTGAATCTAT TGATGAGTTT 
AATAAATCTG CTACTTCTGG TGATATTTGG CCTGGCCTTT CAGCTTATGA TAATAGTCCA 
AGGTCACCTC ATAGTCCTTC ACCTATTGCT ACACCACCCA GTCAGAGTTC ATCATGCTCA 
GATGCCCCCA TGCTTAGTAC AGTGCACTCT GCCAAAAATA CCCCTTCTCA GCATTCACAT 
TCCATTCAGC ACAGTCCTGA GAGGTCTGGA TCTGGCTCTG TTGGAAATGG GTCGAGTCGA 
TATAGTCCTT CTCAGAATAG TCCGATTCAT CACATCCCAT CACGAAGAAG CCCTGCCAAG 
ACAATCACAC CACAGAATGC TCCAAGAGAG GAATCTAGGG GGCGGTCCTC CTTTTATCCT 
GAAGGAGATC AGGAAACAGC AAAGACAGGA AAGTTTTTAA AAAGGTTCAC AGATGAAGAG 
TCTAGAGTAT TCCTGCTTGA TAGGGGTAAT ATCAGGGATA AAGAGGCTCC GAAGGAGAAA 
GGGTCAGAGA AAGGCAGGGC AGATGGAGAT TGGGATGACC AGGAAGTTCT AGATTACTTT 
AGTGATAAAG AGTCTGCAAA ACAAAAATTC CATGACTCTG AAGGGGATGA CACAGAGGAG 
ACAGAGGATT ATAGACAGTT TAGGAAATCA GTCCTAGCAG ATCAGGGCAA GAGCTTTGCT 
ACTTCATCTC ACCGGAATAC TGAGGAGGAA GGACCCAAGT ACAAGTCCAA AGTTTCATTA 
AAAGGCAATA GAGAAAGTGA TGGATTTAGA GAAGAAAAAA ATTATAAACT GAAAGAGACT 
GCATACATAG TCGAAAGGCC TAGCACTGCA AAGGACAAGC ACAAGGAAGA AGACAAAGGC 
TCTGATAGAA TAACAGTGAA GAAAGAAGTG CAGTCACCGG AGCAGGTAAA GTCTGAAAAG 
CTCAAAGAGC TCTTTGATTA CAGTCCCCCT CTACACAAGA GTCTGGATGC ACGAGAAAAG 
TCCATCTTCA GAGAGGAGAG CCCACTGAGG ATCAAAATGA TAGCCAGTGA TTCTCACCGT 
CCTGAAGTCA AACTCAAAAT GGCACCTGTT CCTCTTGACG ATTCCAACAG ACCTGCCTCC 
TTGACTAAAG ACAGGCTACT TGCTAGCACG CTTGTCCATT CTGTCAAGAA AGAGCAAGAA 
TTCCGATCCA TCTTTGACCA CATTAAGCTG CCTCAGGCCA ACAAAAGCAC TTCAGAATCA 
TTTATACAAC ACATTGTATC CTTGGTTCAT CATGTGAAAG AACAATACTT CAAATCACCT 
GCAGTGACCC TAAACGAGCG GTTCACTTCA TACCAGAAAG CTACTGAAGA ACATAGTACC 
CGGCAAAAGA GCCCCGAGAT ACACAGGAGG ATTGACATCT CTCCCAGCGC TCTGAGGAAG 
CACACCCGGC TAGCAGGAGA GGAGAGAGGT TTTAAAGAAG AAATTCAGAA GGGAGATAAA 
AAGTTAAGGT GTGATTCGGC TGACCTTCGG CACGACATTG ACCGTCGCCG TAAAGAGAGA 
AGTAAAGAGC GGGGAGATTC CAAGGGCTCC AGGGAGTCCA GTGGGTCCAG AAAGCAAGAA 
AAAACACCAA AGGATTACAA GGAGTATAAA CCTTACAAAG ATGACAGCAA ACATAAAGGT 
AGAGAGCGGG ACCATTCCCG GTCATCGTCC TCGTCAGCAT CGCCGTCCTC GCCCAGTTCT 
CGAGAAGAAA AAGAGAGTAA GAAAGAGAGA GAAGAAGAAT TTAAAACTCA CCACGAGATG 
AAAGACTACT CAGGATTTGC AGGAGTTAGC AGACCACGAG GAACCTTTTT TCGAATTAGA 
GGCAGAGGAA GAGCCAGAGG AGTTTTTGCT GGGACAAATA CTGGTCCAAA CAACTCAAAT 
ACCACTTTTC AAAAGAGACC GAAGGAAGAG GAATGGGATC CAGAATATAC CCCAAAGAGC 
AAGAAGTACT TCTTGCACGA TGACAGAGAT GATGGTGTGG ATTACTGGGC CAAAAGAGGA 
AGAGGTCGTG GTACTTTTCA ACGTGGCAGA GGGCGCTTTA ATTTCAAAAA ATCAGGTAGC 
AGTCCAAAAT GGACTCATGA CAAATACCAA GGAGATGGGA TTGTTGAAGA TGACGAAGAG 
ACCATGGAAA ATAATGAAGA GAAGAAGGAC AGACGCAAAG AAGAAAAGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( XM_006512865.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512928.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X1, mRNA.

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CloneID	OMu43966
Clone ID Related Accession (Same CDS sequence)	XM_006512865.3 , XM_006512865.4
Accession Version	XM_006512865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGGTCTCG TTCCAGAACA 
TATTCGAGGT CTCGTAGTAG AGATCGTATT TATTCTAGAG ATTATCGTCG AGATTACAGG 
AATAATAGAG GAATGAGACG ACCTTATGGG TACAGAGGAA GGGGTAGAGG GTATTATCAA 
GGAGGAGGAG GGAGATACCA TCGAGGTGGC TATAGACCTG TCTGGAATAG AAGGCACTCT 
AGGAGTCCTA GACGAGGTCG GTCACGGTCC AGGAGTCCAA AAAGAAGATC CGTGTCTTCT 
CAAAGATCCC GAAGCAGATC TCGCCGGTCA TATAGATCCT CTAGGTCTCC AAGATCATCA 
TCATCTCGCT CTTCATCCCC ATATAGCAAA TCTCCTGTCT CTAAAAGACG AGGGTCTCAG 
GAAAAACAAA CCAAAAAAGC TGAGGGGGAA CCCCAAGAAG AGAGTCCTTT GAAAAGCAAA 
TCACAGGAGG AACCAAAGGA TACTTTTGAA CATGATCCAT CTGAATCTAT TGATGAGTTT 
AATAAATCTG CTACTTCTGG TGATATTTGG CCTGGCCTTT CAGCTTATGA TAATAGTCCA 
AGGTCACCTC ATAGTCCTTC ACCTATTGCT ACACCACCCA GTCAGAGTTC ATCATGCTCA 
GATGCCCCCA TGCTTAGTAC AGTGCACTCT GCCAAAAATA CCCCTTCTCA GCATTCACAT 
TCCATTCAGC ACAGTCCTGA GAGGTCTGGA TCTGGCTCTG TTGGAAATGG GTCGAGTCGA 
TATAGTCCTT CTCAGAATAG TCCGATTCAT CACATCCCAT CACGAAGAAG CCCTGCCAAG 
ACAATCACAC CACAGAATGC TCCAAGAGAG GAATCTAGGG GGCGGTCCTC CTTTTATCCT 
GAAGGAGATC AGGAAACAGC AAAGACAGGA AAGTTTTTAA AAAGGTTCAC AGATGAAGAG 
TCTAGAGTAT TCCTGCTTGA TAGGGGTAAT ATCAGGGATA AAGAGGCTCC GAAGGAGAAA 
GGGTCAGAGA AAGGCAGGGC AGATGGAGAT TGGGATGACC AGGAAGTTCT AGATTACTTT 
AGTGATAAAG AGTCTGCAAA ACAAAAATTC CATGACTCTG AAGGGGATGA CACAGAGGAG 
ACAGAGGATT ATAGACAGTT TAGGAAATCA GTCCTAGCAG ATCAGGGCAA GAGCTTTGCT 
ACTTCATCTC ACCGGAATAC TGAGGAGGAA GGACCCAAGT ACAAGTCCAA AGTTTCATTA 
AAAGGCAATA GAGAAAGTGA TGGATTTAGA GAAGAAAAAA ATTATAAACT GAAAGAGACT 
GCATACATAG TCGAAAGGCC TAGCACTGCA AAGGACAAGC ACAAGGAAGA AGACAAAGGC 
TCTGATAGAA TAACAGTGAA GAAAGAAGTG CAGTCACCGG AGCAGGTAAA GTCTGAAAAG 
CTCAAAGAGC TCTTTGATTA CAGTCCCCCT CTACACAAGA GTCTGGATGC ACGAGAAAAG 
TCCATCTTCA GAGAGGAGAG CCCACTGAGG ATCAAAATGA TAGCCAGTGA TTCTCACCGT 
CCTGAAGTCA AACTCAAAAT GGCACCTGTT CCTCTTGACG ATTCCAACAG ACCTGCCTCC 
TTGACTAAAG ACAGGCTACT TGCTAGCACG CTTGTCCATT CTGTCAAGAA AGAGCAAGAA 
TTCCGATCCA TCTTTGACCA CATTAAGCTG CCTCAGGCCA ACAAAAGCAC TTCAGAATCA 
TTTATACAAC ACATTGTATC CTTGGTTCAT CATGTGAAAG AACAATACTT CAAATCACCT 
GCAGTGACCC TAAACGAGCG GTTCACTTCA TACCAGAAAG CTACTGAAGA ACATAGTACC 
CGGCAAAAGA GCCCCGAGAT ACACAGGAGG ATTGACATCT CTCCCAGCGC TCTGAGGAAG 
CACACCCGGC TAGCAGGAGA GGAGAGAGGT TTTAAAGAAG AAATTCAGAA GGGAGATAAA 
AAGTTAAGGT GTGATTCGGC TGACCTTCGG CACGACATTG ACCGTCGCCG TAAAGAGAGA 
AGTAAAGAGC GGGGAGATTC CAAGGGCTCC AGGGAGTCCA GTGGGTCCAG AAAGCAAGAA 
AAAACACCAA AGGATTACAA GGAGTATAAA CCTTACAAAG ATGACAGCAA ACATAAAGGT 
AGAGAGCGGG ACCATTCCCG GTCATCGTCC TCGTCAGCAT CGCCGTCCTC GCCCAGTTCT 
CGAGAAGAAA AAGAGAGTAA GAAAGAGAGA GAAGAAGAAT TTAAAACTCA CCACGAGATG 
AAAGACTACT CAGGATTTGC AGGAGTTAGC AGACCACGAG GAACCTTTCA CGATGACAGA 
GATGATGGTG TGGATTACTG GGCCAAAAGA GGAAGAGGTC GTGGTACTTT TCAACGTGGC 
AGAGGGCGCT TTAATTTCAA AAAATCAGGT AGCAGTCCAA AATGGACTCA TGACAAATAC 
CAAGGAGATG GGATTGTTGA AGATGACGAA GAGACCATGG AAAATAATGA AGAGAAGAAG 
GACAGACGCA AAGAAGAAAA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512928.1
CDS	2208..4814
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKDYSGFAGVSRPRGTFHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKDRRKEEKE

Target ORF information:

RefSeq Version	XM_006512865.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512865.4

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGGTCTCG TTCCAGAACA 
TATTCGAGGT CTCGTAGTAG AGATCGTATT TATTCTAGAG ATTATCGTCG AGATTACAGG 
AATAATAGAG GAATGAGACG ACCTTATGGG TACAGAGGAA GGGGTAGAGG GTATTATCAA 
GGAGGAGGAG GGAGATACCA TCGAGGTGGC TATAGACCTG TCTGGAATAG AAGGCACTCT 
AGGAGTCCTA GACGAGGTCG GTCACGGTCC AGGAGTCCAA AAAGAAGATC CGTGTCTTCT 
CAAAGATCCC GAAGCAGATC TCGCCGGTCA TATAGATCCT CTAGGTCTCC AAGATCATCA 
TCATCTCGCT CTTCATCCCC ATATAGCAAA TCTCCTGTCT CTAAAAGACG AGGGTCTCAG 
GAAAAACAAA CCAAAAAAGC TGAGGGGGAA CCCCAAGAAG AGAGTCCTTT GAAAAGCAAA 
TCACAGGAGG AACCAAAGGA TACTTTTGAA CATGATCCAT CTGAATCTAT TGATGAGTTT 
AATAAATCTG CTACTTCTGG TGATATTTGG CCTGGCCTTT CAGCTTATGA TAATAGTCCA 
AGGTCACCTC ATAGTCCTTC ACCTATTGCT ACACCACCCA GTCAGAGTTC ATCATGCTCA 
GATGCCCCCA TGCTTAGTAC AGTGCACTCT GCCAAAAATA CCCCTTCTCA GCATTCACAT 
TCCATTCAGC ACAGTCCTGA GAGGTCTGGA TCTGGCTCTG TTGGAAATGG GTCGAGTCGA 
TATAGTCCTT CTCAGAATAG TCCGATTCAT CACATCCCAT CACGAAGAAG CCCTGCCAAG 
ACAATCACAC CACAGAATGC TCCAAGAGAG GAATCTAGGG GGCGGTCCTC CTTTTATCCT 
GAAGGAGATC AGGAAACAGC AAAGACAGGA AAGTTTTTAA AAAGGTTCAC AGATGAAGAG 
TCTAGAGTAT TCCTGCTTGA TAGGGGTAAT ATCAGGGATA AAGAGGCTCC GAAGGAGAAA 
GGGTCAGAGA AAGGCAGGGC AGATGGAGAT TGGGATGACC AGGAAGTTCT AGATTACTTT 
AGTGATAAAG AGTCTGCAAA ACAAAAATTC CATGACTCTG AAGGGGATGA CACAGAGGAG 
ACAGAGGATT ATAGACAGTT TAGGAAATCA GTCCTAGCAG ATCAGGGCAA GAGCTTTGCT 
ACTTCATCTC ACCGGAATAC TGAGGAGGAA GGACCCAAGT ACAAGTCCAA AGTTTCATTA 
AAAGGCAATA GAGAAAGTGA TGGATTTAGA GAAGAAAAAA ATTATAAACT GAAAGAGACT 
GCATACATAG TCGAAAGGCC TAGCACTGCA AAGGACAAGC ACAAGGAAGA AGACAAAGGC 
TCTGATAGAA TAACAGTGAA GAAAGAAGTG CAGTCACCGG AGCAGGTAAA GTCTGAAAAG 
CTCAAAGAGC TCTTTGATTA CAGTCCCCCT CTACACAAGA GTCTGGATGC ACGAGAAAAG 
TCCATCTTCA GAGAGGAGAG CCCACTGAGG ATCAAAATGA TAGCCAGTGA TTCTCACCGT 
CCTGAAGTCA AACTCAAAAT GGCACCTGTT CCTCTTGACG ATTCCAACAG ACCTGCCTCC 
TTGACTAAAG ACAGGCTACT TGCTAGCACG CTTGTCCATT CTGTCAAGAA AGAGCAAGAA 
TTCCGATCCA TCTTTGACCA CATTAAGCTG CCTCAGGCCA ACAAAAGCAC TTCAGAATCA 
TTTATACAAC ACATTGTATC CTTGGTTCAT CATGTGAAAG AACAATACTT CAAATCACCT 
GCAGTGACCC TAAACGAGCG GTTCACTTCA TACCAGAAAG CTACTGAAGA ACATAGTACC 
CGGCAAAAGA GCCCCGAGAT ACACAGGAGG ATTGACATCT CTCCCAGCGC TCTGAGGAAG 
CACACCCGGC TAGCAGGAGA GGAGAGAGGT TTTAAAGAAG AAATTCAGAA GGGAGATAAA 
AAGTTAAGGT GTGATTCGGC TGACCTTCGG CACGACATTG ACCGTCGCCG TAAAGAGAGA 
AGTAAAGAGC GGGGAGATTC CAAGGGCTCC AGGGAGTCCA GTGGGTCCAG AAAGCAAGAA 
AAAACACCAA AGGATTACAA GGAGTATAAA CCTTACAAAG ATGACAGCAA ACATAAAGGT 
AGAGAGCGGG ACCATTCCCG GTCATCGTCC TCGTCAGCAT CGCCGTCCTC GCCCAGTTCT 
CGAGAAGAAA AAGAGAGTAA GAAAGAGAGA GAAGAAGAAT TTAAAACTCA CCACGAGATG 
AAAGACTACT CAGGATTTGC AGGAGTTAGC AGACCACGAG GAACCTTTCA CGATGACAGA 
GATGATGGTG TGGATTACTG GGCCAAAAGA GGAAGAGGTC GTGGTACTTT TCAACGTGGC 
AGAGGGCGCT TTAATTTCAA AAAATCAGGT AGCAGTCCAA AATGGACTCA TGACAAATAC 
CAAGGAGATG GGATTGTTGA AGATGACGAA GAGACCATGG AAAATAATGA AGAGAAGAAG 
GACAGACGCA AAGAAGAAAA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bclaf1 ( XM_011243207.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241509.1 Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X2, mRNA.

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CloneID	OMu70924
Clone ID Related Accession (Same CDS sequence)	XM_011243207.2 , XM_011243207.3
Accession Version	XM_011243207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bcl-2-associated transcription factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAAAAT 
GTATTTGTCT TTTACACGGC TTATGGTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241509.1
CDS	2208..4460
Translation	MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGNIRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFATSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEVQSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKNVFVFYTAYGG

Target ORF information:

RefSeq Version	XM_011243207.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated transcription factor 1 (Bclaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243207.3

ORF Insert Sequence:

ATGGGCCGCT CCAATTCTAG ATCACATTCT TCAAGATCCA AGTCTAGATC ACAGTCTAGT 
TCTAGATCAA GATCAAGATC ACATTCTAGA AAGAAGAGAT ACAGTTCTAG GTCTCGTTCC 
AGAACATATT CGAGGTCTCG TAGTAGAGAT CGTATTTATT CTAGAGATTA TCGTCGAGAT 
TACAGGAATA ATAGAGGAAT GAGACGACCT TATGGGTACA GAGGAAGGGG TAGAGGGTAT 
TATCAAGGAG GAGGAGGGAG ATACCATCGA GGTGGCTATA GACCTGTCTG GAATAGAAGG 
CACTCTAGGA GTCCTAGACG AGGTCGGTCA CGGTCCAGGA GTCCAAAAAG AAGATCCGTG 
TCTTCTCAAA GATCCCGAAG CAGATCTCGC CGGTCATATA GATCCTCTAG GTCTCCAAGA 
TCATCATCAT CTCGCTCTTC ATCCCCATAT AGCAAATCTC CTGTCTCTAA AAGACGAGGG 
TCTCAGGAAA AACAAACCAA AAAAGCTGAG GGGGAACCCC AAGAAGAGAG TCCTTTGAAA 
AGCAAATCAC AGGAGGAACC AAAGGATACT TTTGAACATG ATCCATCTGA ATCTATTGAT 
GAGTTTAATA AATCTGCTAC TTCTGGTGAT ATTTGGCCTG GCCTTTCAGC TTATGATAAT 
AGTCCAAGGT CACCTCATAG TCCTTCACCT ATTGCTACAC CACCCAGTCA GAGTTCATCA 
TGCTCAGATG CCCCCATGCT TAGTACAGTG CACTCTGCCA AAAATACCCC TTCTCAGCAT 
TCACATTCCA TTCAGCACAG TCCTGAGAGG TCTGGATCTG GCTCTGTTGG AAATGGGTCG 
AGTCGATATA GTCCTTCTCA GAATAGTCCG ATTCATCACA TCCCATCACG AAGAAGCCCT 
GCCAAGACAA TCACACCACA GAATGCTCCA AGAGAGGAAT CTAGGGGGCG GTCCTCCTTT 
TATCCTGAAG GAGATCAGGA AACAGCAAAG ACAGGAAAGT TTTTAAAAAG GTTCACAGAT 
GAAGAGTCTA GAGTATTCCT GCTTGATAGG GGTAATATCA GGGATAAAGA GGCTCCGAAG 
GAGAAAGGGT CAGAGAAAGG CAGGGCAGAT GGAGATTGGG ATGACCAGGA AGTTCTAGAT 
TACTTTAGTG ATAAAGAGTC TGCAAAACAA AAATTCCATG ACTCTGAAGG GGATGACACA 
GAGGAGACAG AGGATTATAG ACAGTTTAGG AAATCAGTCC TAGCAGATCA GGGCAAGAGC 
TTTGCTACTT CATCTCACCG GAATACTGAG GAGGAAGGAC CCAAGTACAA GTCCAAAGTT 
TCATTAAAAG GCAATAGAGA AAGTGATGGA TTTAGAGAAG AAAAAAATTA TAAACTGAAA 
GAGACTGCAT ACATAGTCGA AAGGCCTAGC ACTGCAAAGG ACAAGCACAA GGAAGAAGAC 
AAAGGCTCTG ATAGAATAAC AGTGAAGAAA GAAGTGCAGT CACCGGAGCA GGTAAAGTCT 
GAAAAGCTCA AAGAGCTCTT TGATTACAGT CCCCCTCTAC ACAAGAGTCT GGATGCACGA 
GAAAAGTCCA TCTTCAGAGA GGAGAGCCCA CTGAGGATCA AAATGATAGC CAGTGATTCT 
CACCGTCCTG AAGTCAAACT CAAAATGGCA CCTGTTCCTC TTGACGATTC CAACAGACCT 
GCCTCCTTGA CTAAAGACAG GCTACTTGCT AGCACGCTTG TCCATTCTGT CAAGAAAGAG 
CAAGAATTCC GATCCATCTT TGACCACATT AAGCTGCCTC AGGCCAACAA AAGCACTTCA 
GAATCATTTA TACAACACAT TGTATCCTTG GTTCATCATG TGAAAGAACA ATACTTCAAA 
TCACCTGCAG TGACCCTAAA CGAGCGGTTC ACTTCATACC AGAAAGCTAC TGAAGAACAT 
AGTACCCGGC AAAAGAGCCC CGAGATACAC AGGAGGATTG ACATCTCTCC CAGCGCTCTG 
AGGAAGCACA CCCGGCTAGC AGGAGAGGAG AGAGGTTTTA AAGAAGAAAT TCAGAAGGGA 
GATAAAAAGT TAAGGTGTGA TTCGGCTGAC CTTCGGCACG ACATTGACCG TCGCCGTAAA 
GAGAGAAGTA AAGAGCGGGG AGATTCCAAG GGCTCCAGGG AGTCCAGTGG GTCCAGAAAG 
CAAGAAAAAA CACCAAAGGA TTACAAGGAG TATAAACCTT ACAAAGATGA CAGCAAAAAT 
GTATTTGTCT TTTACACGGC TTATGGTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Constitutive expression of the anti-apoptotic Bcl-2 family member A1 in murine endothelial cells leads to transplant tolerance.
Clinical and experimental immunology188(2)219-225(2017 May)
Smyth LA,Meader L,Xiao F,Woodward M,Brady HJ,Lechler R,Lombardi G

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing.
Cell reports13(8)1647-57(2015 Nov)
Wei N,Cheng Y,Wang Z,Liu Y,Luo C,Liu L,Chen L,Xie Z,Lu Y,Feng Y

Involvement of Bcl-2-associated transcription factor 1 in the differentiation of early-born retinal cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(4)1530-41(2014 Jan)
Orieux G,Picault L,Slembrouck A,Roger JE,Guillonneau X,Sahel JA,Saule S,McPherson JP,Goureau O

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Bclaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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