Cd248 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd248
Entrez Gene ID	70445
Full Name	CD248 antigen, endosialin
Synonyms	2610111G01Rik,AI842296,Cd164l1,Tem1
General protein information	Preferred Names CD248 antigen, endosialin Names endosialin CD164 sialomucin-like 1 tumor endothelial marker 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_054042.2	NP_473383.1	endosialin precursor

Pathways from BioSystems

Homologies

	CD248	XP_001110499.1
Homo sapiens (human)	CD248	NP_065137.1
Canis lupus familiaris (dog)	CD248	XP_540833.1
Rattus norvegicus (Norway rat)	LOC100911882	XP_003749087.1
Bos taurus (cattle)	CD248	XP_003588093.1
Mus musculus (house mouse)	Cd248	NP_473383.1
Pan troglodytes (chimpanzee)	CD248	XP_003313194.1
Rattus norvegicus (Norway rat)	Cd248	NP_001099795.1
Rattus norvegicus (Norway rat)	LOC100911932	XP_003749088.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatic stellate cells limit hepatocellular carcinoma progression through the orphan receptor endosialin.
Mogler C, K?nig C, Wieland M, Runge A, Besemfelder E, Komljenovic D, Longerich T, Schirmacher P, Augustin HG
EMBO molecular medicine9(6)741-749(2017 Jun)

Endosialin Promotes Atherosclerosis Through Phenotypic Remodeling of Vascular Smooth Muscle Cells.
Hasanov Z, Ruckdeschel T, K?nig C, Mogler C, Kapel SS, Korn C, Spegg C, Eichwald V, Wieland M, Appak S, Augustin HG
Arteriosclerosis, thrombosis, and vascular biology37(3)495-505(2017 Mar)

Endosialin-Expressing Pericytes Promote Metastatic Dissemination.
Viski C, K?nig C, Kijewska M, Mogler C, Isacke CM, Augustin HG
Cancer research76(18)5313-25(2016 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
West DB, Engelhard EK, Adkisson M, Nava AJ, Kirov JV, Cipollone A, Willis B, Rapp J, de Jong PJ, Lloyd KC
PLoS genetics12(2)e1005691(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endosialin is a potential regulator of phenotypic remodeling of vascular smooth muscle cells contributing to atherosclerosis.
Title: Endosialin Promotes Atherosclerosis Through Phenotypic Remodeling of Vascular Smooth Muscle Cells.

the data demonstrate a critical role for endosialin-expressing primary tumor pericytes in mediating metastatic dissemination and identify endosialin as a promising therapeutic target in breast cancer
Title: Endosialin-Expressing Pericytes Promote Metastatic Dissemination.

Our data show that deletion of CD248 reduces susceptibility to liver fibrosis via an effect on PDGF signaling.
Title: CD248/endosialin critically regulates hepatic stellate cell proliferation during chronic liver injury via a PDGF-regulated mechanism.

suggest that CD248 stromal cells have a pathogenic role in renal fibrosis
Title: Genetic Deletion of the Stromal Cell Marker CD248 (Endosialin) Protects against the Development of Renal Fibrosis.

We conclude that CD248 is required for PDGFRbeta-dependant capillary sprouting but not splitting angiogenesis, and identify a new role for CD248 expressed on pericytes in the early stages of physiological angiogenesis during muscle remodelling.
Title: A differential role for CD248 (Endosialin) in PDGF-mediated skeletal muscle angiogenesis.

The following Cd248 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd248 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58158	NM_054042.2 Latest version!	Mus musculus CD248 antigen, endosialin (Cd248), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd248 ( NM_054042.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_473383.1 Mus musculus CD248 antigen, endosialin (Cd248), mRNA.

Price & Availability↑

CloneID	OMu58158
Clone ID Related Accession (Same CDS sequence)	NM_054042.2
Accession Version	NM_054042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Mus musculus(house mouse)
Product	endosialin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC046318.1. On Jun 19, 2003 this sequence version replaced NM_054042.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC046318.1 [ECO:0000345] ##Evidence-Data-END##

ATGCTGCTGC GCCTGCTGCT GGCCTGGGTG GCCGCGGTGC CCGCACTGGG CCAGGTCCCC 
TGGACGCCGG AGCCTCGAGC CGCGTGCGGC CCCAGCAGCT GCTACGCGCT CTTTCCCCGG 
CGCCGCACAT TCCTGGAAGC TTGGCGGGCG TGCCGCGAAT TGGGGGGCAA CCTGGCCACA 
CCGCGGACCC CAGAGGAGGC CCAGCGTGTG GACAGCCTGG TGGGGGTCGG GCCGGCCAAC 
GGGCTGCTAT GGATTGGGTT GCAGCGGCAG GCTAGGCAAT GCCAGCCGCA GCGCCCACTG 
CGGGGCTTCA TATGGACCAC GGGAGACCAG GACACCGCCT TCACCAACTG GGCCCAGCCG 
GCTACGGAAG GACCCTGCCC AGCCCAGCGC TGTGCAGCCC TTGAGGCCAG CGGAGAGCAT 
CGCTGGCTCG AAGGCTCGTG CACACTGGCT GTCGATGGCT ACCTCTGCCA GTTTGGTTTT 
GAGGGTGCCT GCCCTGCCTT GCCGCTTGAG GTGGGTCAGG CCGGTCCCGC TGTCTACACC 
ACACCCTTCA ACCTGGTTTC CAGCGAGTTC GAATGGCTGC CCTTTGGCTC CGTGGCAGCT 
GTGCAGTGCC AAGCTGGCAG GGGAGCTTCT CTGCTGTGCG TGAAACAGCC TTCAGGTGGC 
GTGGGCTGGT CCCAGACTGG CCCGCTGTGC CCAGGGACTG GCTGTGGTCC TGACAATGGG 
GGTTGCGAAC ATGAGTGTGT GGAAGAGGTG GACGGTGCTG TGTCCTGCCG CTGCAGTGAA 
GGCTTCCGTC TAGCAGCAGA TGGGCACAGT TGTGAAGACC CCTGTGCCCA GGCCCCCTGT 
GAGCAGCAGT GTGAACCTGG AGGGCCACAA GGCTATAGCT GCCACTGTCG CCTTGGCTTC 
CGGCCAGCTG AGGATGATCC ACACCGCTGC GTGGACACGG ATGAGTGCCA GATTGCTGGT 
GTGTGCCAGC AGATGTGTGT CAACTATGTT GGTGGCTTTG AGTGTTACTG CAGCGAGGGT 
CACGAGCTTG AGGCAGATGG TATCAGCTGT AGCCCTGCAG GAGCCATGGG TGCCCAGGCT 
TCCCAGGATC TCAGAGATGA GTTGCTGGAT GATGGAGAAG AAGGGGAGGA TGAAGAGGAG 
CCCTGGGAGG ACTTTGATGG CACCTGGACA GAGGAACAGG GGATCCTATG GCTGGCACCT 
ACACATCCAC CTGACTTTGG CCTGCCCTAT AGGCCCAACT TCCCACAGGA TGGAGAGCCT 
CAGAGATTGC ACCTGGAGCC TACCTGGCCA CCCCCACTTA GTGCCCCCAG GGGCCCCTAC 
CACTCCTCAG TGGTGTCTGC CACACGGCCC ATGGTGATCT CTGCCACTCG ACCCACACTA 
CCTTCTGCCC ACAAGACCTC TGTTATTTCA GCTACACGCC CACCCCTGAG CCCTGTCCAC 
CCACCTGCCA TGGCCCCTGC CACACCTCCA GCTGTGTTCT CTGAGCACCA GATCCCCAAA 
ATCAAGGCCA ATTATCCAGA CCTGCCTTTT GGCCACAAGC CTGGGATAAC CTCGGCCACT 
CACCCAGCAC GGTCTCCTCC GTACCAGCCC CCCATTATCT CAACCAACTA TCCCCAAGTC 
TTCCCTCCCC ACCAGGCCCC TATGTCTCCA GATACCCACA CTATCACTTA TTTGCCTCCA 
GTCCCCCCTC ACCTTGATCC TGGGGATACC ACTTCTAAAG CCCATCAACA CCCTTTGCTC 
CCAGATGCTC CAGGTATCAG AACCCAGGCC CCCCAGCTTT CTGTCTCAGC TCTCCAGCCC 
CCTCTTCCTA CCAACTCCAG GTCTTCTGTC CATGAAACTC CTGTGCCTGC TGCCAACCAG 
CCCCCAGCCT TCCCTTCTTC TCCCCTCCCC CCTCAGAGGC CCACTAACCA GACCTCATCT 
ATCAGCCCTA CACATTCCTA TTCCAGAGCC CCTCTAGTCC CAAGGGAAGG AGTTCCCAGT 
CCCAAATCAG TGCCACAGCT GCCCTCGGTG CCCTCCACAG CAGCTCCAAC AGCCCTGGCA 
GAGTCAGGTC TTGCAGGCCA AAGCCAAAGG GATGACCGCT GGCTGCTGGT GGCACTCCTG 
GTGCCAACAT GTGTCTTCTT GGTGGTGCTG CTTGCCCTGG GCATTGTGTA CTGCACTCGC 
TGTGGCTCCC ACGCACCCAA CAAGCGGATC ACGGACTGCT ATCGCTGGGT CACACATGCT 
GGGAACAAGA GCTCAACAGA ACCCATGCCC CCCAGAGGCA GCCTTACAGG GGTACAGACC 
TGTAGAACCA GTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473383.1
CDS	49..2346
Translation	MLLRLLLAWVAAVPALGQVPWTPEPRAACGPSSCYALFPRRRTFLEAWRACRELGGNLATPRTPEEAQRVDSLVGVGPANGLLWIGLQRQARQCQPQRPLRGFIWTTGDQDTAFTNWAQPATEGPCPAQRCAALEASGEHRWLEGSCTLAVDGYLCQFGFEGACPALPLEVGQAGPAVYTTPFNLVSSEFEWLPFGSVAAVQCQAGRGASLLCVKQPSGGVGWSQTGPLCPGTGCGPDNGGCEHECVEEVDGAVSCRCSEGFRLAADGHSCEDPCAQAPCEQQCEPGGPQGYSCHCRLGFRPAEDDPHRCVDTDECQIAGVCQQMCVNYVGGFECYCSEGHELEADGISCSPAGAMGAQASQDLRDELLDDGEEGEDEEEPWEDFDGTWTEEQGILWLAPTHPPDFGLPYRPNFPQDGEPQRLHLEPTWPPPLSAPRGPYHSSVVSATRPMVISATRPTLPSAHKTSVISATRPPLSPVHPPAMAPATPPAVFSEHQIPKIKANYPDLPFGHKPGITSATHPARSPPYQPPIISTNYPQVFPPHQAPMSPDTHTITYLPPVPPHLDPGDTTSKAHQHPLLPDAPGIRTQAPQLSVSALQPPLPTNSRSSVHETPVPAANQPPAFPSSPLPPQRPTNQTSSISPTHSYSRAPLVPREGVPSPKSVPQLPSVPSTAAPTALAESGLAGQSQRDDRWLLVALLVPTCVFLVVLLALGIVYCTRCGSHAPNKRITDCYRWVTHAGNKSSTEPMPPRGSLTGVQTCRTSV

Target ORF information:

RefSeq Version	NM_054042.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD248 antigen, endosialin (Cd248), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054042.2

ORF Insert Sequence:

ATGCTGCTGC GCCTGCTGCT GGCCTGGGTG GCCGCGGTGC CCGCACTGGG CCAGGTCCCC 
TGGACGCCGG AGCCTCGAGC CGCGTGCGGC CCCAGCAGCT GCTACGCGCT CTTTCCCCGG 
CGCCGCACAT TCCTGGAAGC TTGGCGGGCG TGCCGCGAAT TGGGGGGCAA CCTGGCCACA 
CCGCGGACCC CAGAGGAGGC CCAGCGTGTG GACAGCCTGG TGGGGGTCGG GCCGGCCAAC 
GGGCTGCTAT GGATTGGGTT GCAGCGGCAG GCTAGGCAAT GCCAGCCGCA GCGCCCACTG 
CGGGGCTTCA TATGGACCAC GGGAGACCAG GACACCGCCT TCACCAACTG GGCCCAGCCG 
GCTACGGAAG GACCCTGCCC AGCCCAGCGC TGTGCAGCCC TTGAGGCCAG CGGAGAGCAT 
CGCTGGCTCG AAGGCTCGTG CACACTGGCT GTCGATGGCT ACCTCTGCCA GTTTGGTTTT 
GAGGGTGCCT GCCCTGCCTT GCCGCTTGAG GTGGGTCAGG CCGGTCCCGC TGTCTACACC 
ACACCCTTCA ACCTGGTTTC CAGCGAGTTC GAATGGCTGC CCTTTGGCTC CGTGGCAGCT 
GTGCAGTGCC AAGCTGGCAG GGGAGCTTCT CTGCTGTGCG TGAAACAGCC TTCAGGTGGC 
GTGGGCTGGT CCCAGACTGG CCCGCTGTGC CCAGGGACTG GCTGTGGTCC TGACAATGGG 
GGTTGCGAAC ATGAGTGTGT GGAAGAGGTG GACGGTGCTG TGTCCTGCCG CTGCAGTGAA 
GGCTTCCGTC TAGCAGCAGA TGGGCACAGT TGTGAAGACC CCTGTGCCCA GGCCCCCTGT 
GAGCAGCAGT GTGAACCTGG AGGGCCACAA GGCTATAGCT GCCACTGTCG CCTTGGCTTC 
CGGCCAGCTG AGGATGATCC ACACCGCTGC GTGGACACGG ATGAGTGCCA GATTGCTGGT 
GTGTGCCAGC AGATGTGTGT CAACTATGTT GGTGGCTTTG AGTGTTACTG CAGCGAGGGT 
CACGAGCTTG AGGCAGATGG TATCAGCTGT AGCCCTGCAG GAGCCATGGG TGCCCAGGCT 
TCCCAGGATC TCAGAGATGA GTTGCTGGAT GATGGAGAAG AAGGGGAGGA TGAAGAGGAG 
CCCTGGGAGG ACTTTGATGG CACCTGGACA GAGGAACAGG GGATCCTATG GCTGGCACCT 
ACACATCCAC CTGACTTTGG CCTGCCCTAT AGGCCCAACT TCCCACAGGA TGGAGAGCCT 
CAGAGATTGC ACCTGGAGCC TACCTGGCCA CCCCCACTTA GTGCCCCCAG GGGCCCCTAC 
CACTCCTCAG TGGTGTCTGC CACACGGCCC ATGGTGATCT CTGCCACTCG ACCCACACTA 
CCTTCTGCCC ACAAGACCTC TGTTATTTCA GCTACACGCC CACCCCTGAG CCCTGTCCAC 
CCACCTGCCA TGGCCCCTGC CACACCTCCA GCTGTGTTCT CTGAGCACCA GATCCCCAAA 
ATCAAGGCCA ATTATCCAGA CCTGCCTTTT GGCCACAAGC CTGGGATAAC CTCGGCCACT 
CACCCAGCAC GGTCTCCTCC GTACCAGCCC CCCATTATCT CAACCAACTA TCCCCAAGTC 
TTCCCTCCCC ACCAGGCCCC TATGTCTCCA GATACCCACA CTATCACTTA TTTGCCTCCA 
GTCCCCCCTC ACCTTGATCC TGGGGATACC ACTTCTAAAG CCCATCAACA CCCTTTGCTC 
CCAGATGCTC CAGGTATCAG AACCCAGGCC CCCCAGCTTT CTGTCTCAGC TCTCCAGCCC 
CCTCTTCCTA CCAACTCCAG GTCTTCTGTC CATGAAACTC CTGTGCCTGC TGCCAACCAG 
CCCCCAGCCT TCCCTTCTTC TCCCCTCCCC CCTCAGAGGC CCACTAACCA GACCTCATCT 
ATCAGCCCTA CACATTCCTA TTCCAGAGCC CCTCTAGTCC CAAGGGAAGG AGTTCCCAGT 
CCCAAATCAG TGCCACAGCT GCCCTCGGTG CCCTCCACAG CAGCTCCAAC AGCCCTGGCA 
GAGTCAGGTC TTGCAGGCCA AAGCCAAAGG GATGACCGCT GGCTGCTGGT GGCACTCCTG 
GTGCCAACAT GTGTCTTCTT GGTGGTGCTG CTTGCCCTGG GCATTGTGTA CTGCACTCGC 
TGTGGCTCCC ACGCACCCAA CAAGCGGATC ACGGACTGCT ATCGCTGGGT CACACATGCT 
GGGAACAAGA GCTCAACAGA ACCCATGCCC CCCAGAGGCA GCCTTACAGG GGTACAGACC 
TGTAGAACCA GTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatic stellate cells limit hepatocellular carcinoma progression through the orphan receptor endosialin.
EMBO molecular medicine9(6)741-749(2017 Jun)
Mogler C,K?nig C,Wieland M,Runge A,Besemfelder E,Komljenovic D,Longerich T,Schirmacher P,Augustin HG

Endosialin Promotes Atherosclerosis Through Phenotypic Remodeling of Vascular Smooth Muscle Cells.
Arteriosclerosis, thrombosis, and vascular biology37(3)495-505(2017 Mar)
Hasanov Z,Ruckdeschel T,K?nig C,Mogler C,Kapel SS,Korn C,Spegg C,Eichwald V,Wieland M,Appak S,Augustin HG

Endosialin-Expressing Pericytes Promote Metastatic Dissemination.
Cancer research76(18)5313-25(2016 Sep)
Viski C,K?nig C,Kijewska M,Mogler C,Isacke CM,Augustin HG

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
PLoS genetics12(2)e1005691(2016 Feb)
West DB,Engelhard EK,Adkisson M,Nava AJ,Kirov JV,Cipollone A,Willis B,Rapp J,de Jong PJ,Lloyd KC

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Cd248 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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