Vps53 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps53
Entrez Gene ID	68299
Full Name	VPS53 GARP complex subunit
Synonyms	2010002A08Rik,2310040I21Rik,3100002B05Rik,Hccs1
General protein information	Preferred Names VPS53 GARP complex subunit Names vacuolar protein sorting-associated protein 53 homolog vacuolar protein sorting 53
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534053.3	XP_006534116.1	vacuolar protein sorting-associated protein 53 homolog isoform X2
NM_026664.4	NP_080940.2	vacuolar protein sorting-associated protein 53 homolog isoform 1
XM_006534052.3	XP_006534115.1	vacuolar protein sorting-associated protein 53 homolog isoform X1
NM_001364738.1	NP_001351667.1	vacuolar protein sorting-associated protein 53 homolog isoform 2
NM_026664.3	NP_080940.2	vacuolar protein sorting-associated protein 53 homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VPS53	NP_001180097.1
Danio rerio (zebrafish)	vps53	NP_001007459.2
Arabidopsis thaliana (thale cress)	AT1G50970	NP_175510.2
Gallus gallus (chicken)	VPS53	NP_001012824.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006623	XP_316653.4
Caenorhabditis elegans (roundworm)	vps-53	NP_001255027.1
Magnaporthe oryzae (rice blast fungus)	MGG_01331	XP_003714256.1
Canis lupus familiaris (dog)	VPS53	XP_548308.2
Mus musculus (house mouse)	Vps53	NP_080940.2
Rattus norvegicus (Norway rat)	Vps53	NP_001099283.1
Neurospora crassa	NCU03024	XP_965410.1
Arabidopsis thaliana (thale cress)	HIT1	NP_564573.3
Xenopus tropicalis (tropical clawed frog)	LOC100496110	XP_002934389.2
Pan troglodytes (chimpanzee)	VPS53	XP_001151796.1
Schizosaccharomyces pombe (fission yeast)	vps53	NP_593341.1
Oryza sativa (rice)	Os01g0906200	NP_001045132.2
Homo sapiens (human)	VPS53	NP_001121631.1
Drosophila melanogaster (fruit fly)	CG3338	NP_608825.2

Gene Ontology
Bibliography

Related articles in PubMed

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

Loss of vps54 function leads to vesicle traffic impairment, protein mis-sorting and embryonic lethality.
Karlsson P, Droce A, Moser JM, Cuhlmann S, Padilla CO, Heimann P, Bartsch JW, F?chtbauer A, F?chtbauer EM, Schmitt-John T
International journal of molecular sciences14(6)10908-25(2013 May)

HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality.
Gan Y, Zhao X, Hu J, Wang ZG, Zhao XT
Cell death and differentiation15(9)1481-90(2008 Sep)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Disruption of HCCS1 in mice leads to embryonic lethality, accompanied by abnormal labyrinth architecture resulting from the excessive proliferation of trophoblast cells in the placenta.
Title: HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality.

The following Vps53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84049	NM_001364738.1 Latest version!	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu02771	NM_026664.4 Latest version!	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00
OMu46824	XM_006534052.3 Latest version!	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu46825	XM_006534053.3 Latest version!	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu02771	NM_026664.3	Mus musculus VPS53 GARP complex subunit (Vps53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps53 ( NM_001364738.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351667.1 Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84049
Clone ID Related Accession (Same CDS sequence)	NM_001364738.1
Accession Version	NM_001364738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-11
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK009586.1, AK008026.1 and AL591129.24. ##Evidence-Data-START## Transcript exon combination :: AK008026.1, BY708014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTTGTG GAGGAGCTGG AGGCTGTGCT GCAGCTCACG 
CCAGAGGTGC AACTCGCCAT CGAGCAGGTA TTCCCAAGCC AGGATCCTCT TGATCAAGCT 
GACTTCAATG CCGTTGAGTA TATCAATACT CTGTTCCCAA CAGAGCAAGT GACAAATGAC 
CTCAATCTGT TCTTAAGAAC ACATCTATTT CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351667.1
CDS	140..355
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDQADFNAVEYINTLFPTEQVTNDLNLFLRTHLFP

Target ORF information:

RefSeq Version	NM_001364738.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364738.1

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTTGTG GAGGAGCTGG AGGCTGTGCT GCAGCTCACG 
CCAGAGGTGC AACTCGCCAT CGAGCAGGTA TTCCCAAGCC AGGATCCTCT TGATCAAGCT 
GACTTCAATG CCGTTGAGTA TATCAATACT CTGTTCCCAA CAGAGCAAGT GACAAATGAC 
CTCAATCTGT TCTTAAGAAC ACATCTATTT CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps53 ( NM_026664.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080940.2 Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02771
Clone ID Related Accession (Same CDS sequence)	NM_026664.4 , NM_026664.3
Accession Version	NM_026664.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK009586.1 and AK033495.1. On Jul 3, 2018 this sequence version replaced NM_026664.3. ##Evidence-Data-START## Transcript exon combination :: AK033495.1, AK019408.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGAGG AGGAGGAACT GGAGTTTGTG GAGGAGCTGG AGGCTGTGCT GCAGCTCACG 
CCAGAGGTGC AACTCGCCAT CGAGCAGGTA TTCCCAAGCC AGGATCCTCT TGATCAAGCT 
GACTTCAATG CCGTTGAGTA TATCAATACT CTGTTCCCAA CAGAGCAATC TCTGGCAAAC 
ATTGACGATG TTGTGAACAA AATTAGACTG AAAATAAGGA GACTGGATGA CAATATCCGG 
ACTGTCGTAA GAGGTCAGAC AAATGTGGGG CAGGATGGAA GGCAAGCACT TGAAGAGGCC 
CAGAAAGCCA TTCAGCAACT CTTTGGCAAA ATCAAGGATA TCAAAGACAA AGCAGAAAAG 
TCAGAACAAA TGGTGAAAGA GATCACCCGA GATATTAAGC AGTTAGATCA CGCCAAGCGC 
CACCTGACCA CCTCAATCAC GACCCTGAAC CACCTGCACA TGCTGGCAGG CGGGGTGGAC 
TCCCTGGAAG CCATGACCAG GCGCAGACAG TATGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTAATGAATG TTCTGGAGCA CTTTCACAAA TACATGGGAA TCCCACAGAT CCGACAGCTT 
TCTGAAAGGG TGAAGGCTGC ACAGACAGAG TTAGGACAGC AAATCCTGGC TGACTTTGAA 
GAAGCATTTC CTTCGCAGGG TACCAAGAGG CCGGGAGGAC CCAGCAATGT CCTGAGAGAT 
GCGTGCCTGG TGGCTAATAT TTTGGACCCC AGAATCAAAC AAGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTATCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TTGACAGACG TTATGCTTGG GTGAAACGCC AGCTTGTGGA CTATGAAGAG 
AAGTATGGCC GGATGTTTCC TCGGGAGTGG TGCATGACGG AGAGGATCTC AGTAGAGTTT 
TGCCACGTGA CGAGGGCAGA ACTTTCCAAA ATCATGCGTG CCAGAGCTAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC GATCCAGAGA ACAACCAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GTTGCACCCT GACAGATGGA ACCCTGAAAA AGCTGGAGTC CCCACCACCA 
TCTACCAATC CTTTCCTGGA AGATGAGACA ACACCAGAGA TGGAGGAGCT GGCTCTGGAG 
AAAGGAGAGT TAGAGCAACC GAAGAAGCCC AAGGCCCCGG ACAACCCGTT CCACGGCATC 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTACATTG AGTCCCAGGA CAAAAACCTC 
AGCGAGCTGA TAGACCGGTT TGTGGCAGAT TTCAAAGCCC AGGGCCCACC CAAGCCCAAC 
ACTGACGAAG GGGGTGCTGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCACA GCTCAGCACA GGCGAGCCCA TGATCGCCCT GACTACCATC 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCTAAAACC 
AGCAGCAGCA GTGGGGGGCT GACCATCAGC AGCCTCCTCA AAGAGAAGGA GGGCTCAGAA 
GTGGCCAGGT TCACTCTGGA GGAGCTGTGT CTCATCTGCA GCATCCTGAG CACAGCCGAG 
TATTGTCTGG CTACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTGAGC 
CTGACTGAAC GAATTAATCT CACAGGAGAA ATGGACACAT TCAGCACTGT CATCTCCAGC 
AGCATCCAGC TGTTGGTTCA AGATCTGGAT GCAGCCTGTG ACCCTGCCCT GATTGCCATG 
AGCAAGATGC CTTGGCAGAA CGTGGAGCAT GTTGGTGACC AGAGCCCTTA TGTCACTTCT 
GTTATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGG 
AAATACTTCA CACAGTTCTG CATTAAATTT GCAAATTCGT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGTAAGCC GATCAGCATG GTGGGAGCAG AGCAGCTTCT GCTGGACACC 
CACTCCCTGA AGATGGTCCT GCTCGACCTT CCGTCCATTG GCTCCCAGGT GGTGAGGAAG 
GCCCCTGCCA GTTACACTAA GATTGTCGTG AAAGGCATGA CCCGGGCTGA AATGATCCTT 
AAGGTGGTGA TGGCCCCTCA TGAACCGTTG GTGGTATTTG TTGACAACTA CATCAAACTC 
CTTACGGACT GCAACTCAGA AACCTTCCAG AAAATACTGG ATATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTTTTG CGGCAGAGGC TCCCTGCCCC GCCCTCGGGG 
ACAGAAGGTT CCAGCACCCT GTCGCTGATT GCCCCAACAC CGGAGCAGGA GTCATCCCGA 
ATCCGCAAAC TGGAGAAACT GATTAAAAAG AGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080940.2
CDS	140..2638
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDQADFNAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWVKRQLVDYEEKYGRMFPREWCMTERISVEFCHVTRAELSKIMRARAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDETTPEMEELALEKGELEQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTSSSSGGLTISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLTERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSSTLSLIAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	NM_026664.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026664.4

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTTGTG GAGGAGCTGG AGGCTGTGCT GCAGCTCACG 
CCAGAGGTGC AACTCGCCAT CGAGCAGGTA TTCCCAAGCC AGGATCCTCT TGATCAAGCT 
GACTTCAATG CCGTTGAGTA TATCAATACT CTGTTCCCAA CAGAGCAATC TCTGGCAAAC 
ATTGACGATG TTGTGAACAA AATTAGACTG AAAATAAGGA GACTGGATGA CAATATCCGG 
ACTGTCGTAA GAGGTCAGAC AAATGTGGGG CAGGATGGAA GGCAAGCACT TGAAGAGGCC 
CAGAAAGCCA TTCAGCAACT CTTTGGCAAA ATCAAGGATA TCAAAGACAA AGCAGAAAAG 
TCAGAACAAA TGGTGAAAGA GATCACCCGA GATATTAAGC AGTTAGATCA CGCCAAGCGC 
CACCTGACCA CCTCAATCAC GACCCTGAAC CACCTGCACA TGCTGGCAGG CGGGGTGGAC 
TCCCTGGAAG CCATGACCAG GCGCAGACAG TATGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTAATGAATG TTCTGGAGCA CTTTCACAAA TACATGGGAA TCCCACAGAT CCGACAGCTT 
TCTGAAAGGG TGAAGGCTGC ACAGACAGAG TTAGGACAGC AAATCCTGGC TGACTTTGAA 
GAAGCATTTC CTTCGCAGGG TACCAAGAGG CCGGGAGGAC CCAGCAATGT CCTGAGAGAT 
GCGTGCCTGG TGGCTAATAT TTTGGACCCC AGAATCAAAC AAGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTATCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TTGACAGACG TTATGCTTGG GTGAAACGCC AGCTTGTGGA CTATGAAGAG 
AAGTATGGCC GGATGTTTCC TCGGGAGTGG TGCATGACGG AGAGGATCTC AGTAGAGTTT 
TGCCACGTGA CGAGGGCAGA ACTTTCCAAA ATCATGCGTG CCAGAGCTAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC GATCCAGAGA ACAACCAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GTTGCACCCT GACAGATGGA ACCCTGAAAA AGCTGGAGTC CCCACCACCA 
TCTACCAATC CTTTCCTGGA AGATGAGACA ACACCAGAGA TGGAGGAGCT GGCTCTGGAG 
AAAGGAGAGT TAGAGCAACC GAAGAAGCCC AAGGCCCCGG ACAACCCGTT CCACGGCATC 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTACATTG AGTCCCAGGA CAAAAACCTC 
AGCGAGCTGA TAGACCGGTT TGTGGCAGAT TTCAAAGCCC AGGGCCCACC CAAGCCCAAC 
ACTGACGAAG GGGGTGCTGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCACA GCTCAGCACA GGCGAGCCCA TGATCGCCCT GACTACCATC 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCTAAAACC 
AGCAGCAGCA GTGGGGGGCT GACCATCAGC AGCCTCCTCA AAGAGAAGGA GGGCTCAGAA 
GTGGCCAGGT TCACTCTGGA GGAGCTGTGT CTCATCTGCA GCATCCTGAG CACAGCCGAG 
TATTGTCTGG CTACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTGAGC 
CTGACTGAAC GAATTAATCT CACAGGAGAA ATGGACACAT TCAGCACTGT CATCTCCAGC 
AGCATCCAGC TGTTGGTTCA AGATCTGGAT GCAGCCTGTG ACCCTGCCCT GATTGCCATG 
AGCAAGATGC CTTGGCAGAA CGTGGAGCAT GTTGGTGACC AGAGCCCTTA TGTCACTTCT 
GTTATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGG 
AAATACTTCA CACAGTTCTG CATTAAATTT GCAAATTCGT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGTAAGCC GATCAGCATG GTGGGAGCAG AGCAGCTTCT GCTGGACACC 
CACTCCCTGA AGATGGTCCT GCTCGACCTT CCGTCCATTG GCTCCCAGGT GGTGAGGAAG 
GCCCCTGCCA GTTACACTAA GATTGTCGTG AAAGGCATGA CCCGGGCTGA AATGATCCTT 
AAGGTGGTGA TGGCCCCTCA TGAACCGTTG GTGGTATTTG TTGACAACTA CATCAAACTC 
CTTACGGACT GCAACTCAGA AACCTTCCAG AAAATACTGG ATATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTTTTG CGGCAGAGGC TCCCTGCCCC GCCCTCGGGG 
ACAGAAGGTT CCAGCACCCT GTCGCTGATT GCCCCAACAC CGGAGCAGGA GTCATCCCGA 
ATCCGCAAAC TGGAGAAACT GATTAAAAAG AGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps53 ( NM_026664.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080940.2 Mus musculus VPS53 GARP complex subunit (Vps53), mRNA.

Price & Availability↑

CloneID	OMu02771
Clone ID Related Accession (Same CDS sequence)	NM_026664.4 , NM_026664.3
Accession Version	NM_026664.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 53 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK033495.1. On Apr 6, 2007 this sequence version replaced NM_026664.2. ##Evidence-Data-START## Transcript exon combination :: AK033495.1, AK019408.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTTGTG GAGGAGCTGG AGGCTGTGCT GCAGCTCACG 
CCAGAGGTGC AACTCGCCAT CGAGCAGGTA TTCCCAAGCC AGGATCCTCT TGATCAAGCT 
GACTTCAATG CCGTTGAGTA TATCAATACT CTGTTCCCAA CAGAGCAATC TCTGGCAAAC 
ATTGACGATG TTGTGAACAA AATTAGACTG AAAATAAGGA GACTGGATGA CAATATCCGG 
ACTGTCGTAA GAGGTCAGAC AAATGTGGGG CAGGATGGAA GGCAAGCACT TGAAGAGGCC 
CAGAAAGCCA TTCAGCAACT CTTTGGCAAA ATCAAGGATA TCAAAGACAA AGCAGAAAAG 
TCAGAACAAA TGGTGAAAGA GATCACCCGA GATATTAAGC AGTTAGATCA CGCCAAGCGC 
CACCTGACCA CCTCAATCAC GACCCTGAAC CACCTGCACA TGCTGGCAGG CGGGGTGGAC 
TCCCTGGAAG CCATGACCAG GCGCAGACAG TATGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTAATGAATG TTCTGGAGCA CTTTCACAAA TACATGGGAA TCCCACAGAT CCGACAGCTT 
TCTGAAAGGG TGAAGGCTGC ACAGACAGAG TTAGGACAGC AAATCCTGGC TGACTTTGAA 
GAAGCATTTC CTTCGCAGGG TACCAAGAGG CCGGGAGGAC CCAGCAATGT CCTGAGAGAT 
GCGTGCCTGG TGGCTAATAT TTTGGACCCC AGAATCAAAC AAGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTATCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TTGACAGACG TTATGCTTGG GTGAAACGCC AGCTTGTGGA CTATGAAGAG 
AAGTATGGCC GGATGTTTCC TCGGGAGTGG TGCATGACGG AGAGGATCTC AGTAGAGTTT 
TGCCACGTGA CGAGGGCAGA ACTTTCCAAA ATCATGCGTG CCAGAGCTAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC GATCCAGAGA ACAACCAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GTTGCACCCT GACAGATGGA ACCCTGAAAA AGCTGGAGTC CCCACCACCA 
TCTACCAATC CTTTCCTGGA AGATGAGACA ACACCAGAGA TGGAGGAGCT GGCTCTGGAG 
AAAGGAGAGT TAGAGCAACC GAAGAAGCCC AAGGCCCCGG ACAACCCGTT CCACGGCATC 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTACATTG AGTCCCAGGA CAAAAACCTC 
AGCGAGCTGA TAGACCGGTT TGTGGCAGAT TTCAAAGCCC AGGGCCCACC CAAGCCCAAC 
ACTGACGAAG GGGGTGCTGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCACA GCTCAGCACA GGCGAGCCCA TGATCGCCCT GACTACCATC 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCTAAAACC 
AGCAGCAGCA GTGGGGGGCT GACCATCAGC AGCCTCCTCA AAGAGAAGGA GGGCTCAGAA 
GTGGCCAGGT TCACTCTGGA GGAGCTGTGT CTCATCTGCA GCATCCTGAG CACAGCCGAG 
TATTGTCTGG CTACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTGAGC 
CTGACTGAAC GAATTAATCT CACAGGAGAA ATGGACACAT TCAGCACTGT CATCTCCAGC 
AGCATCCAGC TGTTGGTTCA AGATCTGGAT GCAGCCTGTG ACCCTGCCCT GATTGCCATG 
AGCAAGATGC CTTGGCAGAA CGTGGAGCAT GTTGGTGACC AGAGCCCTTA TGTCACTTCT 
GTTATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGG 
AAATACTTCA CACAGTTCTG CATTAAATTT GCAAATTCGT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGTAAGCC GATCAGCATG GTGGGAGCAG AGCAGCTTCT GCTGGACACC 
CACTCCCTGA AGATGGTCCT GCTCGACCTT CCGTCCATTG GCTCCCAGGT GGTGAGGAAG 
GCCCCTGCCA GTTACACTAA GATTGTCGTG AAAGGCATGA CCCGGGCTGA AATGATCCTT 
AAGGTGGTGA TGGCCCCTCA TGAACCGTTG GTGGTATTTG TTGACAACTA CATCAAACTC 
CTTACGGACT GCAACTCAGA AACCTTCCAG AAAATACTGG ATATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTTTTG CGGCAGAGGC TCCCTGCCCC GCCCTCGGGG 
ACAGAAGGTT CCAGCACCCT GTCGCTGATT GCCCCAACAC CGGAGCAGGA GTCATCCCGA 
ATCCGCAAAC TGGAGAAACT GATTAAAAAG AGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080940.2
CDS	132..2630
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDQADFNAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWVKRQLVDYEEKYGRMFPREWCMTERISVEFCHVTRAELSKIMRARAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDETTPEMEELALEKGELEQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTSSSSGGLTISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLTERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSSTLSLIAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	NM_026664.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS53 GARP complex subunit (Vps53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026664.3

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTTGTG GAGGAGCTGG AGGCTGTGCT GCAGCTCACG 
CCAGAGGTGC AACTCGCCAT CGAGCAGGTA TTCCCAAGCC AGGATCCTCT TGATCAAGCT 
GACTTCAATG CCGTTGAGTA TATCAATACT CTGTTCCCAA CAGAGCAATC TCTGGCAAAC 
ATTGACGATG TTGTGAACAA AATTAGACTG AAAATAAGGA GACTGGATGA CAATATCCGG 
ACTGTCGTAA GAGGTCAGAC AAATGTGGGG CAGGATGGAA GGCAAGCACT TGAAGAGGCC 
CAGAAAGCCA TTCAGCAACT CTTTGGCAAA ATCAAGGATA TCAAAGACAA AGCAGAAAAG 
TCAGAACAAA TGGTGAAAGA GATCACCCGA GATATTAAGC AGTTAGATCA CGCCAAGCGC 
CACCTGACCA CCTCAATCAC GACCCTGAAC CACCTGCACA TGCTGGCAGG CGGGGTGGAC 
TCCCTGGAAG CCATGACCAG GCGCAGACAG TATGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTAATGAATG TTCTGGAGCA CTTTCACAAA TACATGGGAA TCCCACAGAT CCGACAGCTT 
TCTGAAAGGG TGAAGGCTGC ACAGACAGAG TTAGGACAGC AAATCCTGGC TGACTTTGAA 
GAAGCATTTC CTTCGCAGGG TACCAAGAGG CCGGGAGGAC CCAGCAATGT CCTGAGAGAT 
GCGTGCCTGG TGGCTAATAT TTTGGACCCC AGAATCAAAC AAGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTATCAGA GTATCTAGTA CTTTTTCAAG AAAACCAAGA TGTCGCCTGG 
CTGGACAAAA TTGACAGACG TTATGCTTGG GTGAAACGCC AGCTTGTGGA CTATGAAGAG 
AAGTATGGCC GGATGTTTCC TCGGGAGTGG TGCATGACGG AGAGGATCTC AGTAGAGTTT 
TGCCACGTGA CGAGGGCAGA ACTTTCCAAA ATCATGCGTG CCAGAGCTAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC GATCCAGAGA ACAACCAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GTTGCACCCT GACAGATGGA ACCCTGAAAA AGCTGGAGTC CCCACCACCA 
TCTACCAATC CTTTCCTGGA AGATGAGACA ACACCAGAGA TGGAGGAGCT GGCTCTGGAG 
AAAGGAGAGT TAGAGCAACC GAAGAAGCCC AAGGCCCCGG ACAACCCGTT CCACGGCATC 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTACATTG AGTCCCAGGA CAAAAACCTC 
AGCGAGCTGA TAGACCGGTT TGTGGCAGAT TTCAAAGCCC AGGGCCCACC CAAGCCCAAC 
ACTGACGAAG GGGGTGCTGT GCTCCCCAGC TGCGCTGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AGTGCTCACA GCTCAGCACA GGCGAGCCCA TGATCGCCCT GACTACCATC 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCTAAAACC 
AGCAGCAGCA GTGGGGGGCT GACCATCAGC AGCCTCCTCA AAGAGAAGGA GGGCTCAGAA 
GTGGCCAGGT TCACTCTGGA GGAGCTGTGT CTCATCTGCA GCATCCTGAG CACAGCCGAG 
TATTGTCTGG CTACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT TGATGTGAGC 
CTGACTGAAC GAATTAATCT CACAGGAGAA ATGGACACAT TCAGCACTGT CATCTCCAGC 
AGCATCCAGC TGTTGGTTCA AGATCTGGAT GCAGCCTGTG ACCCTGCCCT GATTGCCATG 
AGCAAGATGC CTTGGCAGAA CGTGGAGCAT GTTGGTGACC AGAGCCCTTA TGTCACTTCT 
GTTATTCTTC ACATTAAGCA AAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGG 
AAATACTTCA CACAGTTCTG CATTAAATTT GCAAATTCGT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGTAAGCC GATCAGCATG GTGGGAGCAG AGCAGCTTCT GCTGGACACC 
CACTCCCTGA AGATGGTCCT GCTCGACCTT CCGTCCATTG GCTCCCAGGT GGTGAGGAAG 
GCCCCTGCCA GTTACACTAA GATTGTCGTG AAAGGCATGA CCCGGGCTGA AATGATCCTT 
AAGGTGGTGA TGGCCCCTCA TGAACCGTTG GTGGTATTTG TTGACAACTA CATCAAACTC 
CTTACGGACT GCAACTCAGA AACCTTCCAG AAAATACTGG ATATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTTTTG CGGCAGAGGC TCCCTGCCCC GCCCTCGGGG 
ACAGAAGGTT CCAGCACCCT GTCGCTGATT GCCCCAACAC CGGAGCAGGA GTCATCCCGA 
ATCCGCAAAC TGGAGAAACT GATTAAAAAG AGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps53 ( XM_006534052.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534115.1 Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46824
Clone ID Related Accession (Same CDS sequence)	XM_006534052.3
Accession Version	XM_006534052.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTAA AGGTATTCCC AAGCCAGGAT CCTCTTGATC AAGCTGACTT CAATGCCGTT 
GAGTATATCA ATACTCTGTT CCCAACAGAG CAATCTCTGG CAAACATTGA CGATGTTGTG 
AACAAAATTA GACTGAAAAT AAGGAGACTG GATGACAATA TCCGGACTGT CGTAAGAGGT 
CAGACAAATG TGGGGCAGGA TGGAAGGCAA GCACTTGAAG AGGCCCAGAA AGCCATTCAG 
CAACTCTTTG GCAAAATCAA GGATATCAAA GACAAAGCAG AAAAGTCAGA ACAAATGGTG 
AAAGAGATCA CCCGAGATAT TAAGCAGTTA GATCACGCCA AGCGCCACCT GACCACCTCA 
ATCACGACCC TGAACCACCT GCACATGCTG GCAGGCGGGG TGGACTCCCT GGAAGCCATG 
ACCAGGCGCA GACAGTATGG AGAAGTTGCT AATCTCCTTC AGGGTGTAAT GAATGTTCTG 
GAGCACTTTC ACAAATACAT GGGAATCCCA CAGATCCGAC AGCTTTCTGA AAGGGTGAAG 
GCTGCACAGA CAGAGTTAGG ACAGCAAATC CTGGCTGACT TTGAAGAAGC ATTTCCTTCG 
CAGGGTACCA AGAGGCCGGG AGGACCCAGC AATGTCCTGA GAGATGCGTG CCTGGTGGCT 
AATATTTTGG ACCCCAGAAT CAAACAAGAA ATCATCAAAA AGTTTATTAA ACAGCATCTA 
TCAGAGTATC TAGTACTTTT TCAAGAAAAC CAAGATGTCG CCTGGCTGGA CAAAATTGAC 
AGACGTTATG CTTGGGTGAA ACGCCAGCTT GTGGACTATG AAGAGAAGTA TGGCCGGATG 
TTTCCTCGGG AGTGGTGCAT GACGGAGAGG ATCTCAGTAG AGTTTTGCCA CGTGACGAGG 
GCAGAACTTT CCAAAATCAT GCGTGCCAGA GCTAAGGAAA TTGAAGTGAA ATTGCTTCTT 
TTTGCGATCC AGAGAACAAC CAACTTTGAG GGGTTTCTTG CAAAACGCTT CTCCGGTTGC 
ACCCTGACAG ATGGAACCCT GAAAAAGCTG GAGTCCCCAC CACCATCTAC CAATCCTTTC 
CTGGAAGATG AGACAACACC AGAGATGGAG GAGCTGGCTC TGGAGAAAGG AGAGTTAGAG 
CAACCGAAGA AGCCCAAGGC CCCGGACAAC CCGTTCCACG GCATCGTTTC CAAGTGTTTT 
GAGCCTCATC TCTACGTGTA CATTGAGTCC CAGGACAAAA ACCTCAGCGA GCTGATAGAC 
CGGTTTGTGG CAGATTTCAA AGCCCAGGGC CCACCCAAGC CCAACACTGA CGAAGGGGGT 
GCTGTGCTCC CCAGCTGCGC TGACCTCTTT GTCTACTACA AGAAGTGCAT GGTGCAGTGC 
TCACAGCTCA GCACAGGCGA GCCCATGATC GCCCTGACTA CCATCTTCCA GAAGTACCTC 
CGAGAATACG CCTGGAAAAT CCTCTCTGGC AACCTGCCTA AAACCAGCAG CAGCAGTGGG 
GGGCTGACCA TCAGCAGCCT CCTCAAAGAG AAGGAGGGCT CAGAAGTGGC CAGGTTCACT 
CTGGAGGAGC TGTGTCTCAT CTGCAGCATC CTGAGCACAG CCGAGTATTG TCTGGCTACC 
ACCCAGCAGC TAGAAGAAAA ACTCAAAGAA AAAGTTGATG TGAGCCTGAC TGAACGAATT 
AATCTCACAG GAGAAATGGA CACATTCAGC ACTGTCATCT CCAGCAGCAT CCAGCTGTTG 
GTTCAAGATC TGGATGCAGC CTGTGACCCT GCCCTGATTG CCATGAGCAA GATGCCTTGG 
CAGAACGTGG AGCATGTTGG TGACCAGAGC CCTTATGTCA CTTCTGTTAT TCTTCACATT 
AAGCAAAACG TCCCCATCAT CCGTGACAAC CTGGCTTCCA CACGGAAATA CTTCACACAG 
TTCTGCATTA AATTTGCAAA TTCGTTCATT CCCAAATTCA TCACCCACCT CTTCAAGTGT 
AAGCCGATCA GCATGGTGGG AGCAGAGCAG CTTCTGCTGG ACACCCACTC CCTGAAGATG 
GTCCTGCTCG ACCTTCCGTC CATTGGCTCC CAGGTGGTGA GGAAGGCCCC TGCCAGTTAC 
ACTAAGATTG TCGTGAAAGG CATGACCCGG GCTGAAATGA TCCTTAAGGT GGTGATGGCC 
CCTCATGAAC CGTTGGTGGT ATTTGTTGAC AACTACATCA AACTCCTTAC GGACTGCAAC 
TCAGAAACCT TCCAGAAAAT ACTGGATATG AAGGGGCTGA AGAGGAGTGA GCAGAGCAGC 
ATGCTGGAAC TTTTGCGGCA GAGGCTCCCT GCCCCGCCCT CGGGGACAGA AGGTTCCAGC 
ACCCTGTCGC TGATTGCCCC AACACCGGAG CAGGAGTCAT CCCGAATCCG CAAACTGGAG 
AAACTGATTA AAAAGAGACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534115.1
CDS	93..2516
Translation	MVVKVFPSQDPLDQADFNAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWVKRQLVDYEEKYGRMFPREWCMTERISVEFCHVTRAELSKIMRARAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDETTPEMEELALEKGELEQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTSSSSGGLTISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLTERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSSTLSLIAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_006534052.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534052.3

ORF Insert Sequence:

ATGGTGGTAA AGGTATTCCC AAGCCAGGAT CCTCTTGATC AAGCTGACTT CAATGCCGTT 
GAGTATATCA ATACTCTGTT CCCAACAGAG CAATCTCTGG CAAACATTGA CGATGTTGTG 
AACAAAATTA GACTGAAAAT AAGGAGACTG GATGACAATA TCCGGACTGT CGTAAGAGGT 
CAGACAAATG TGGGGCAGGA TGGAAGGCAA GCACTTGAAG AGGCCCAGAA AGCCATTCAG 
CAACTCTTTG GCAAAATCAA GGATATCAAA GACAAAGCAG AAAAGTCAGA ACAAATGGTG 
AAAGAGATCA CCCGAGATAT TAAGCAGTTA GATCACGCCA AGCGCCACCT GACCACCTCA 
ATCACGACCC TGAACCACCT GCACATGCTG GCAGGCGGGG TGGACTCCCT GGAAGCCATG 
ACCAGGCGCA GACAGTATGG AGAAGTTGCT AATCTCCTTC AGGGTGTAAT GAATGTTCTG 
GAGCACTTTC ACAAATACAT GGGAATCCCA CAGATCCGAC AGCTTTCTGA AAGGGTGAAG 
GCTGCACAGA CAGAGTTAGG ACAGCAAATC CTGGCTGACT TTGAAGAAGC ATTTCCTTCG 
CAGGGTACCA AGAGGCCGGG AGGACCCAGC AATGTCCTGA GAGATGCGTG CCTGGTGGCT 
AATATTTTGG ACCCCAGAAT CAAACAAGAA ATCATCAAAA AGTTTATTAA ACAGCATCTA 
TCAGAGTATC TAGTACTTTT TCAAGAAAAC CAAGATGTCG CCTGGCTGGA CAAAATTGAC 
AGACGTTATG CTTGGGTGAA ACGCCAGCTT GTGGACTATG AAGAGAAGTA TGGCCGGATG 
TTTCCTCGGG AGTGGTGCAT GACGGAGAGG ATCTCAGTAG AGTTTTGCCA CGTGACGAGG 
GCAGAACTTT CCAAAATCAT GCGTGCCAGA GCTAAGGAAA TTGAAGTGAA ATTGCTTCTT 
TTTGCGATCC AGAGAACAAC CAACTTTGAG GGGTTTCTTG CAAAACGCTT CTCCGGTTGC 
ACCCTGACAG ATGGAACCCT GAAAAAGCTG GAGTCCCCAC CACCATCTAC CAATCCTTTC 
CTGGAAGATG AGACAACACC AGAGATGGAG GAGCTGGCTC TGGAGAAAGG AGAGTTAGAG 
CAACCGAAGA AGCCCAAGGC CCCGGACAAC CCGTTCCACG GCATCGTTTC CAAGTGTTTT 
GAGCCTCATC TCTACGTGTA CATTGAGTCC CAGGACAAAA ACCTCAGCGA GCTGATAGAC 
CGGTTTGTGG CAGATTTCAA AGCCCAGGGC CCACCCAAGC CCAACACTGA CGAAGGGGGT 
GCTGTGCTCC CCAGCTGCGC TGACCTCTTT GTCTACTACA AGAAGTGCAT GGTGCAGTGC 
TCACAGCTCA GCACAGGCGA GCCCATGATC GCCCTGACTA CCATCTTCCA GAAGTACCTC 
CGAGAATACG CCTGGAAAAT CCTCTCTGGC AACCTGCCTA AAACCAGCAG CAGCAGTGGG 
GGGCTGACCA TCAGCAGCCT CCTCAAAGAG AAGGAGGGCT CAGAAGTGGC CAGGTTCACT 
CTGGAGGAGC TGTGTCTCAT CTGCAGCATC CTGAGCACAG CCGAGTATTG TCTGGCTACC 
ACCCAGCAGC TAGAAGAAAA ACTCAAAGAA AAAGTTGATG TGAGCCTGAC TGAACGAATT 
AATCTCACAG GAGAAATGGA CACATTCAGC ACTGTCATCT CCAGCAGCAT CCAGCTGTTG 
GTTCAAGATC TGGATGCAGC CTGTGACCCT GCCCTGATTG CCATGAGCAA GATGCCTTGG 
CAGAACGTGG AGCATGTTGG TGACCAGAGC CCTTATGTCA CTTCTGTTAT TCTTCACATT 
AAGCAAAACG TCCCCATCAT CCGTGACAAC CTGGCTTCCA CACGGAAATA CTTCACACAG 
TTCTGCATTA AATTTGCAAA TTCGTTCATT CCCAAATTCA TCACCCACCT CTTCAAGTGT 
AAGCCGATCA GCATGGTGGG AGCAGAGCAG CTTCTGCTGG ACACCCACTC CCTGAAGATG 
GTCCTGCTCG ACCTTCCGTC CATTGGCTCC CAGGTGGTGA GGAAGGCCCC TGCCAGTTAC 
ACTAAGATTG TCGTGAAAGG CATGACCCGG GCTGAAATGA TCCTTAAGGT GGTGATGGCC 
CCTCATGAAC CGTTGGTGGT ATTTGTTGAC AACTACATCA AACTCCTTAC GGACTGCAAC 
TCAGAAACCT TCCAGAAAAT ACTGGATATG AAGGGGCTGA AGAGGAGTGA GCAGAGCAGC 
ATGCTGGAAC TTTTGCGGCA GAGGCTCCCT GCCCCGCCCT CGGGGACAGA AGGTTCCAGC 
ACCCTGTCGC TGATTGCCCC AACACCGGAG CAGGAGTCAT CCCGAATCCG CAAACTGGAG 
AAACTGATTA AAAAGAGACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps53 ( XM_006534053.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534116.1 Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46825
Clone ID Related Accession (Same CDS sequence)	XM_006534053.3
Accession Version	XM_006534053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG AGATCACCCG AGATATTAAG CAGTTAGATC ACGCCAAGCG CCACCTGACC 
ACCTCAATCA CGACCCTGAA CCACCTGCAC ATGCTGGCAG GCGGGGTGGA CTCCCTGGAA 
GCCATGACCA GGCGCAGACA GTATGGAGAA GTTGCTAATC TCCTTCAGGG TGTAATGAAT 
GTTCTGGAGC ACTTTCACAA ATACATGGGA ATCCCACAGA TCCGACAGCT TTCTGAAAGG 
GTGAAGGCTG CACAGACAGA GTTAGGACAG CAAATCCTGG CTGACTTTGA AGAAGCATTT 
CCTTCGCAGG GTACCAAGAG GCCGGGAGGA CCCAGCAATG TCCTGAGAGA TGCGTGCCTG 
GTGGCTAATA TTTTGGACCC CAGAATCAAA CAAGAAATCA TCAAAAAGTT TATTAAACAG 
CATCTATCAG AGTATCTAGT ACTTTTTCAA GAAAACCAAG ATGTCGCCTG GCTGGACAAA 
ATTGACAGAC GTTATGCTTG GGTGAAACGC CAGCTTGTGG ACTATGAAGA GAAGTATGGC 
CGGATGTTTC CTCGGGAGTG GTGCATGACG GAGAGGATCT CAGTAGAGTT TTGCCACGTG 
ACGAGGGCAG AACTTTCCAA AATCATGCGT GCCAGAGCTA AGGAAATTGA AGTGAAATTG 
CTTCTTTTTG CGATCCAGAG AACAACCAAC TTTGAGGGGT TTCTTGCAAA ACGCTTCTCC 
GGTTGCACCC TGACAGATGG AACCCTGAAA AAGCTGGAGT CCCCACCACC ATCTACCAAT 
CCTTTCCTGG AAGATGAGAC AACACCAGAG ATGGAGGAGC TGGCTCTGGA GAAAGGAGAG 
TTAGAGCAAC CGAAGAAGCC CAAGGCCCCG GACAACCCGT TCCACGGCAT CGTTTCCAAG 
TGTTTTGAGC CTCATCTCTA CGTGTACATT GAGTCCCAGG ACAAAAACCT CAGCGAGCTG 
ATAGACCGGT TTGTGGCAGA TTTCAAAGCC CAGGGCCCAC CCAAGCCCAA CACTGACGAA 
GGGGGTGCTG TGCTCCCCAG CTGCGCTGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG 
CAGTGCTCAC AGCTCAGCAC AGGCGAGCCC ATGATCGCCC TGACTACCAT CTTCCAGAAG 
TACCTCCGAG AATACGCCTG GAAAATCCTC TCTGGCAACC TGCCTAAAAC CAGCAGCAGC 
AGTGGGGGGC TGACCATCAG CAGCCTCCTC AAAGAGAAGG AGGGCTCAGA AGTGGCCAGG 
TTCACTCTGG AGGAGCTGTG TCTCATCTGC AGCATCCTGA GCACAGCCGA GTATTGTCTG 
GCTACCACCC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TTGATGTGAG CCTGACTGAA 
CGAATTAATC TCACAGGAGA AATGGACACA TTCAGCACTG TCATCTCCAG CAGCATCCAG 
CTGTTGGTTC AAGATCTGGA TGCAGCCTGT GACCCTGCCC TGATTGCCAT GAGCAAGATG 
CCTTGGCAGA ACGTGGAGCA TGTTGGTGAC CAGAGCCCTT ATGTCACTTC TGTTATTCTT 
CACATTAAGC AAAACGTCCC CATCATCCGT GACAACCTGG CTTCCACACG GAAATACTTC 
ACACAGTTCT GCATTAAATT TGCAAATTCG TTCATTCCCA AATTCATCAC CCACCTCTTC 
AAGTGTAAGC CGATCAGCAT GGTGGGAGCA GAGCAGCTTC TGCTGGACAC CCACTCCCTG 
AAGATGGTCC TGCTCGACCT TCCGTCCATT GGCTCCCAGG TGGTGAGGAA GGCCCCTGCC 
AGTTACACTA AGATTGTCGT GAAAGGCATG ACCCGGGCTG AAATGATCCT TAAGGTGGTG 
ATGGCCCCTC ATGAACCGTT GGTGGTATTT GTTGACAACT ACATCAAACT CCTTACGGAC 
TGCAACTCAG AAACCTTCCA GAAAATACTG GATATGAAGG GGCTGAAGAG GAGTGAGCAG 
AGCAGCATGC TGGAACTTTT GCGGCAGAGG CTCCCTGCCC CGCCCTCGGG GACAGAAGGT 
TCCAGCACCC TGTCGCTGAT TGCCCCAACA CCGGAGCAGG AGTCATCCCG AATCCGCAAA 
CTGGAGAAAC TGATTAAAAA GAGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534116.1
CDS	1509..3638
Translation	MVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWVKRQLVDYEEKYGRMFPREWCMTERISVEFCHVTRAELSKIMRARAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDETTPEMEELALEKGELEQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTSSSSGGLTISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLTERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSSTLSLIAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_006534053.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534053.3

ORF Insert Sequence:

ATGGTGAAAG AGATCACCCG AGATATTAAG CAGTTAGATC ACGCCAAGCG CCACCTGACC 
ACCTCAATCA CGACCCTGAA CCACCTGCAC ATGCTGGCAG GCGGGGTGGA CTCCCTGGAA 
GCCATGACCA GGCGCAGACA GTATGGAGAA GTTGCTAATC TCCTTCAGGG TGTAATGAAT 
GTTCTGGAGC ACTTTCACAA ATACATGGGA ATCCCACAGA TCCGACAGCT TTCTGAAAGG 
GTGAAGGCTG CACAGACAGA GTTAGGACAG CAAATCCTGG CTGACTTTGA AGAAGCATTT 
CCTTCGCAGG GTACCAAGAG GCCGGGAGGA CCCAGCAATG TCCTGAGAGA TGCGTGCCTG 
GTGGCTAATA TTTTGGACCC CAGAATCAAA CAAGAAATCA TCAAAAAGTT TATTAAACAG 
CATCTATCAG AGTATCTAGT ACTTTTTCAA GAAAACCAAG ATGTCGCCTG GCTGGACAAA 
ATTGACAGAC GTTATGCTTG GGTGAAACGC CAGCTTGTGG ACTATGAAGA GAAGTATGGC 
CGGATGTTTC CTCGGGAGTG GTGCATGACG GAGAGGATCT CAGTAGAGTT TTGCCACGTG 
ACGAGGGCAG AACTTTCCAA AATCATGCGT GCCAGAGCTA AGGAAATTGA AGTGAAATTG 
CTTCTTTTTG CGATCCAGAG AACAACCAAC TTTGAGGGGT TTCTTGCAAA ACGCTTCTCC 
GGTTGCACCC TGACAGATGG AACCCTGAAA AAGCTGGAGT CCCCACCACC ATCTACCAAT 
CCTTTCCTGG AAGATGAGAC AACACCAGAG ATGGAGGAGC TGGCTCTGGA GAAAGGAGAG 
TTAGAGCAAC CGAAGAAGCC CAAGGCCCCG GACAACCCGT TCCACGGCAT CGTTTCCAAG 
TGTTTTGAGC CTCATCTCTA CGTGTACATT GAGTCCCAGG ACAAAAACCT CAGCGAGCTG 
ATAGACCGGT TTGTGGCAGA TTTCAAAGCC CAGGGCCCAC CCAAGCCCAA CACTGACGAA 
GGGGGTGCTG TGCTCCCCAG CTGCGCTGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG 
CAGTGCTCAC AGCTCAGCAC AGGCGAGCCC ATGATCGCCC TGACTACCAT CTTCCAGAAG 
TACCTCCGAG AATACGCCTG GAAAATCCTC TCTGGCAACC TGCCTAAAAC CAGCAGCAGC 
AGTGGGGGGC TGACCATCAG CAGCCTCCTC AAAGAGAAGG AGGGCTCAGA AGTGGCCAGG 
TTCACTCTGG AGGAGCTGTG TCTCATCTGC AGCATCCTGA GCACAGCCGA GTATTGTCTG 
GCTACCACCC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TTGATGTGAG CCTGACTGAA 
CGAATTAATC TCACAGGAGA AATGGACACA TTCAGCACTG TCATCTCCAG CAGCATCCAG 
CTGTTGGTTC AAGATCTGGA TGCAGCCTGT GACCCTGCCC TGATTGCCAT GAGCAAGATG 
CCTTGGCAGA ACGTGGAGCA TGTTGGTGAC CAGAGCCCTT ATGTCACTTC TGTTATTCTT 
CACATTAAGC AAAACGTCCC CATCATCCGT GACAACCTGG CTTCCACACG GAAATACTTC 
ACACAGTTCT GCATTAAATT TGCAAATTCG TTCATTCCCA AATTCATCAC CCACCTCTTC 
AAGTGTAAGC CGATCAGCAT GGTGGGAGCA GAGCAGCTTC TGCTGGACAC CCACTCCCTG 
AAGATGGTCC TGCTCGACCT TCCGTCCATT GGCTCCCAGG TGGTGAGGAA GGCCCCTGCC 
AGTTACACTA AGATTGTCGT GAAAGGCATG ACCCGGGCTG AAATGATCCT TAAGGTGGTG 
ATGGCCCCTC ATGAACCGTT GGTGGTATTT GTTGACAACT ACATCAAACT CCTTACGGAC 
TGCAACTCAG AAACCTTCCA GAAAATACTG GATATGAAGG GGCTGAAGAG GAGTGAGCAG 
AGCAGCATGC TGGAACTTTT GCGGCAGAGG CTCCCTGCCC CGCCCTCGGG GACAGAAGGT 
TCCAGCACCC TGTCGCTGAT TGCCCCAACA CCGGAGCAGG AGTCATCCCG AATCCGCAAA 
CTGGAGAAAC TGATTAAAAA GAGACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

Loss of vps54 function leads to vesicle traffic impairment, protein mis-sorting and embryonic lethality.
International journal of molecular sciences14(6)10908-25(2013 May)
Karlsson P,Droce A,Moser JM,Cuhlmann S,Padilla CO,Heimann P,Bartsch JW,F?chtbauer A,F?chtbauer EM,Schmitt-John T

HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality.
Cell death and differentiation15(9)1481-90(2008 Sep)
Gan Y,Zhao X,Hu J,Wang ZG,Zhao XT

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Vps53 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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