VPS53 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS53
Entrez Gene ID	55275
Full Name	VPS53, GARP complex subunit
Synonyms	HCCS1,PCH2E,hVps53L,pp13624
General protein information	Preferred Names VPS53, GARP complex subunit Names vacuolar protein sorting-associated protein 53 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.3
Summary	This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008].
Disorder MIM:	615850
Disorder Html:	Pontocerebellar hypoplasia, type 2E, 615851 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128159.2	NP_001121631.1	vacuolar protein sorting-associated protein 53 homolog isoform 1
NM_001366253.2	NP_001353182.1	vacuolar protein sorting-associated protein 53 homolog isoform 3
NM_018289.3	NP_060759.2	vacuolar protein sorting-associated protein 53 homolog isoform 2
XM_017024817.1	XP_016880306.1	vacuolar protein sorting-associated protein 53 homolog isoform X1
NM_001366253.1	NP_001353182.1	vacuolar protein sorting-associated protein 53 homolog isoform 3
XM_017024818.1	XP_016880307.1	vacuolar protein sorting-associated protein 53 homolog isoform X2
NM_018289.4	NP_060759.2	vacuolar protein sorting-associated protein 53 homolog isoform 2
XM_011523953.2	XP_011522255.1	vacuolar protein sorting-associated protein 53 homolog isoform X3
NM_001366254.1	NP_001353183.1	vacuolar protein sorting-associated protein 53 homolog isoform 4
NM_001366254.2	NP_001353183.1	vacuolar protein sorting-associated protein 53 homolog isoform 4
XM_017024817.2	XP_016880306.1	vacuolar protein sorting-associated protein 53 homolog isoform X1
NM_001128159.3	NP_001121631.1	vacuolar protein sorting-associated protein 53 homolog isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VPS53	NP_001180097.1
Danio rerio (zebrafish)	vps53	NP_001007459.2
Arabidopsis thaliana (thale cress)	AT1G50970	NP_175510.2
Gallus gallus (chicken)	VPS53	NP_001012824.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006623	XP_316653.4
Caenorhabditis elegans (roundworm)	vps-53	NP_001255027.1
Magnaporthe oryzae (rice blast fungus)	MGG_01331	XP_003714256.1
Canis lupus familiaris (dog)	VPS53	XP_548308.2
Mus musculus (house mouse)	Vps53	NP_080940.2
Rattus norvegicus (Norway rat)	Vps53	NP_001099283.1
Neurospora crassa	NCU03024	XP_965410.1
Arabidopsis thaliana (thale cress)	HIT1	NP_564573.3
Xenopus tropicalis (tropical clawed frog)	LOC100496110	XP_002934389.2
Pan troglodytes (chimpanzee)	VPS53	XP_001151796.1
Schizosaccharomyces pombe (fission yeast)	vps53	NP_593341.1
Oryza sativa (rice)	Os01g0906200	NP_001045132.2
Homo sapiens (human)	VPS53	NP_001121631.1
Drosophila melanogaster (fruit fly)	CG3338	NP_608825.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Hepatocellular carcinoma suppressor 1 promoter hypermethylation in serum. A diagnostic and prognostic study in hepatitis B.
Tian MM, Fan YC, Zhao J, Gao S, Zhao ZH, Chen LY, Wang K
Clinics and research in hepatology and gastroenterology41(2)171-180(2017 Mar)

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Eeles RA, Olama AA, Benlloch S, Saunders EJ, Leongamornlert DA, Tymrakiewicz M, Ghoussaini M, Luccarini C, Dennis J, Jugurnauth-Little S, Dadaev T, Neal DE, Hamdy FC, Donovan JL, Muir K, Giles GG, Severi G, Wiklund F, Gronberg H, Haiman CA, Schumacher F, Henderson BE, Le Marchand L, Lindstrom S, Kraft P, Hunter DJ, Gapstur S, Chanock SJ, Berndt SI, Albanes D, Andriole G, Schleutker J, Weischer M, Canzian F, Riboli E, Key TJ, Travis RC, Campa D, Ingles SA, John EM, Hayes RB, Pharoah PD, Pashayan N, Khaw KT, Stanford JL, Ostrander EA, Signorello LB, Thibodeau SN, Schaid D, Maier C, Vogel W, Kibel AS, Cybulski C, Lubinski J, Cannon-Albright L, Brenner H, Park JY, Kaneva R, Batra J, Spurdle AB, Clements JA, Teixeira MR, Dicks E, Lee A, Dunning AM, Baynes C, Conroy D, Maranian MJ, Ahmed S, Govindasami K, Guy M, Wilkinson RA, Sawyer EJ, Morgan A, Dearnaley DP, Horwich A, Huddart RA, Khoo VS, Parker CC, Van As NJ, Woodhouse CJ, Thompson A, Dudderidge T, Ogden C, Cooper CS, Lophatananon A, Cox A, Southey MC, Hopper JL, English DR, Aly M, Adolfsson J, Xu J, Zheng SL, Yeager M, Kaaks R, Diver WR, Gaudet MM, Stern MC, Corral R, Joshi AD, Shahabi A, Wahlfors T, Tammela TL, Auvinen A, Virtamo J, Klarskov P, Nordestgaard BG, R?der MA, Nielsen SF, Bojesen SE, Siddiq A, Fitzgerald LM, Kolb S, Kwon EM, Karyadi DM, Blot WJ, Zheng W, Cai Q, McDonnell SK, Rinckleb AE, Drake B, Colditz G, Wokolorczyk D, Stephenson RA, Teerlink C, Muller H, Rothenbacher D, Sellers TA, Lin HY, Slavov C, Mitev V, Lose F, Srinivasan S, Maia S, Paulo P, Lange E, Cooney KA, Antoniou AC, Vincent D, Bacot F, Tessier DC, , , , , , Kote-Jarai Z, Easton DF
Nature genetics45(4)385-91, 391e1-2(2013 Apr)

Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
P?rez-Victoria FJ, Schindler C, Magad?n JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS
Molecular biology of the cell21(19)3386-95(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Golgi-associated retrograde protein orchestrates retrograde transport from endosomes to the trans-Golgi network by promoting vesicle tethering and assembly of SNARE complexes in consecutive, independent steps.[Golgi associated retrograde protein]
Title: Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.

Missorting of cathepsin D in GARP-depleted cells results from accumulation of recycling MPRs in a population of light, small vesicles downstream of endosomes.
Title: Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes.

Amphipathic tail-anchoring peptide (ATAP) targets specifically to mitochondria, and induces caspase-dependent apoptosis that does not require Bax or Bak.
Title: The tail-anchoring domain of Bfl1 and HCCS1 targets mitochondrial membrane permeability to induce apoptosis.

Data show that transcription of the putative tumor suppressor gene HCCS1 requires binding of ETS-2 to its consensus near the transcription start site; observations provide insight into the mechanistic aspects of HCCS1 transcription regulation
Title: Transcription of the putative tumor suppressor gene HCCS1 requires binding of ETS-2 to its consensus near the transcription start site.

Golgi associated retrograde protein complex, consisting of the Vps52, Vps53, and Vps54 proteins, shows significant conservation between various species but diversification and specialization result in important differences in human cells.
Title: Characterization of the human GARP (Golgi associated retrograde protein) complex.

The following VPS53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu69264	NM_001366254.2 Latest version!	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69264	XM_011523953.2 Latest version!	Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu28566	NM_001128159.3 Latest version!	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu28642	NM_018289.4 Latest version!	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu107092	NM_001366253.2 Latest version!	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86964	XM_017024817.2 Latest version!	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86965	XM_017024818.1 Latest version!	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu107092	NM_001366253.1	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu28642	NM_018289.3	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu69264	NM_001366254.1	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu86964	XM_017024817.1	Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu28566	NM_001128159.2	Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS53 ( NM_001366254.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353183.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu69264
Clone ID Related Accession (Same CDS sequence)	XM_011523953.2 , NM_001366254.1 , NM_001366254.2
Accession Version	NM_001366254.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. On Sep 27, 2018 this sequence version replaced XM_011523953.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092532.1 [ECO:0000332] ##Evidence-Data-END##

ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT 
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA 
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA 
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT 
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT 
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG 
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA 
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT 
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA 
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA 
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT 
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG 
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG 
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC 
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC 
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC 
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC 
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG 
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT 
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC 
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT 
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC 
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC 
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC 
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353183.1
CDS	823..2328
Translation	MFSLIVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	NM_001366254.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366254.1

ORF Insert Sequence:

ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT 
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA 
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA 
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT 
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT 
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG 
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA 
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT 
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA 
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA 
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT 
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG 
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG 
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC 
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC 
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC 
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC 
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG 
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT 
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC 
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT 
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC 
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC 
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC 
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_001366254.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353183.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu69264
Clone ID Related Accession (Same CDS sequence)	XM_011523953.2 , NM_001366254.1 , NM_001366254.2
Accession Version	NM_001366254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. On May 31, 2019 this sequence version replaced NM_001366254.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT 
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA 
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA 
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT 
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT 
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG 
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA 
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT 
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA 
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA 
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT 
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG 
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG 
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC 
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC 
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC 
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC 
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG 
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT 
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC 
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT 
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC 
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC 
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC 
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353183.1
CDS	823..2328
Translation	MFSLIVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	NM_001366254.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366254.2

ORF Insert Sequence:

ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT 
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA 
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA 
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT 
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT 
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG 
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA 
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT 
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA 
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA 
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT 
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG 
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG 
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC 
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC 
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC 
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC 
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG 
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT 
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC 
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT 
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC 
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC 
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC 
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_011523953.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522255.1 Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X4, mRNA.

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CloneID	OHu69264
Clone ID Related Accession (Same CDS sequence)	XM_011523953.2 , NM_001366254.1 , NM_001366254.2
Accession Version	XM_011523953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT 
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA 
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA 
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT 
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT 
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG 
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA 
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT 
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA 
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA 
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT 
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG 
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG 
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC 
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC 
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC 
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC 
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG 
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT 
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC 
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT 
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC 
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC 
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC 
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522255.1
CDS	830..2335
Translation	MFSLIVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	XM_011523953.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523953.2

ORF Insert Sequence:

ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT 
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA 
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA 
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT 
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT 
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG 
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA 
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT 
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA 
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA 
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT 
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG 
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG 
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC 
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC 
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC 
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC 
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG 
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT 
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC 
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT 
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC 
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC 
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC 
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_001128159.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121631.1 Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant 1, mRNA.

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CloneID	OHu28566
Clone ID Related Accession (Same CDS sequence)	NM_001128159.2 , NM_001128159.3
Accession Version	NM_001128159.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AL701573.1, EU021218.1, AK001841.1 and AC015853.8. This sequence is a reference standard in the RefSeqGene project. On Aug 11, 2010 this sequence version replaced NM_001128159.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155068.1, SRR1803617.254618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC 
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG 
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC 
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT 
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG 
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC 
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121631.1
CDS	148..2646
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	NM_001128159.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128159.2

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC 
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG 
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC 
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT 
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG 
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC 
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_001128159.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121631.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28566
Clone ID Related Accession (Same CDS sequence)	NM_001128159.2 , NM_001128159.3
Accession Version	NM_001128159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AL701573.1, EU021218.1, AK001841.1 and AC015853.8. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001128159.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155068.1, SRR1803617.254618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000437048.7/ ENSP00000401435.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC 
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG 
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC 
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT 
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG 
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC 
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121631.1
CDS	131..2629
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	NM_001128159.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128159.3

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC 
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG 
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC 
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT 
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG 
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG 
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC 
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_018289.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060759.2 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA.

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CloneID	OHu28642
Clone ID Related Accession (Same CDS sequence)	NM_018289.3 , NM_018289.4
Accession Version	NM_018289.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-27
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AK290343.1 and AK001841.1. On Jun 8, 2008 this sequence version replaced NM_018289.2. Transcript Variant: This variant (2) has multiple differences in the coding region and uses a different segment for its 3' UTR but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290343.1, BC006116.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC 
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG 
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA 
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC 
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT 
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG 
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT 
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG 
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC 
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG 
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC 
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA 
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT 
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG 
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG 
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC 
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT 
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC 
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT 
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC 
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC 
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC 
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA 
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA 
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG 
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG 
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC 
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA 
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC 
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060759.2
CDS	148..2160
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	NM_018289.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018289.3

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC 
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG 
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA 
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC 
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT 
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG 
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT 
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG 
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC 
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG 
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC 
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA 
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT 
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG 
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG 
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC 
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT 
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC 
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT 
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC 
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC 
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC 
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA 
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA 
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG 
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG 
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC 
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA 
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC 
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_018289.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060759.2 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28642
Clone ID Related Accession (Same CDS sequence)	NM_018289.3 , NM_018289.4
Accession Version	NM_018289.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AK290343.1, AK001841.1 and AC015853.8. On May 31, 2019 this sequence version replaced NM_018289.3. Transcript Variant: This variant (2) has multiple differences in the coding region and uses a different segment for its 3' UTR but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290343.1, BC006116.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC 
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG 
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA 
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC 
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT 
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG 
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT 
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG 
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC 
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG 
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC 
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA 
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT 
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG 
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG 
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC 
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT 
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC 
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT 
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC 
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC 
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC 
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA 
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA 
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG 
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG 
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC 
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA 
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC 
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060759.2
CDS	131..2143
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	NM_018289.4
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018289.4

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC 
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG 
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA 
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC 
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT 
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG 
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT 
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG 
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC 
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG 
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC 
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA 
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT 
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG 
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG 
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC 
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT 
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC 
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT 
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC 
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC 
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC 
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA 
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA 
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG 
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG 
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC 
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA 
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC 
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_001366253.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353182.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA.

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CloneID	OHu107092
Clone ID Related Accession (Same CDS sequence)	NM_001366253.2 , NM_001366253.1
Accession Version	NM_001366253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. On May 31, 2019 this sequence version replaced NM_001366253.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.52831.1, SRR1660807.163452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353182.1
CDS	131..2230
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	NM_001366253.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366253.2

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( NM_001366253.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353182.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA.

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CloneID	OHu107092
Clone ID Related Accession (Same CDS sequence)	NM_001366253.2 , NM_001366253.1
Accession Version	NM_001366253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY444797.1, SRR1660805.52831.1 [ECO:0000332] ##Evidence-Data-END##

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353182.1
CDS	131..2230
Translation	MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQVRWT

Target ORF information:

RefSeq Version	NM_001366253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366253.1

ORF Insert Sequence:

ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG 
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA 
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC 
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA 
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT 
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA 
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC 
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC 
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT 
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT 
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA 
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT 
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT 
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG 
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG 
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT 
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA 
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA 
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA 
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG 
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT 
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC 
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC 
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG 
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT 
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC 
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA 
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG 
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT 
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC 
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG 
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT 
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC 
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC 
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_017024817.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880306.1 Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86964
Clone ID Related Accession (Same CDS sequence)	XM_017024817.1 , XM_017024817.2
Accession Version	XM_017024817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC 
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG 
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA 
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT 
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC 
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA 
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG 
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG 
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA 
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC 
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA 
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG 
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG 
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG 
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA 
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG 
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA 
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT 
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC 
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT 
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC 
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT 
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG 
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC 
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC 
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA 
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG 
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT 
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC 
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC 
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT 
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG 
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC 
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC 
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG 
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG 
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG 
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG 
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG 
AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880306.1
CDS	344..2692
Translation	MDGNLAFCLHGRTWPPFSSSLFHPRKQYLEEHRFSVVVVPSPFPCALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_017024817.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024817.1

ORF Insert Sequence:

ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC 
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG 
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA 
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT 
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC 
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA 
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG 
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG 
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA 
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC 
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA 
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG 
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG 
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG 
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA 
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG 
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA 
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT 
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC 
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT 
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC 
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT 
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG 
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC 
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC 
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA 
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG 
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT 
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC 
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC 
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT 
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG 
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC 
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC 
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG 
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG 
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG 
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG 
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG 
AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_017024817.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880306.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86964
Clone ID Related Accession (Same CDS sequence)	XM_017024817.1 , XM_017024817.2
Accession Version	XM_017024817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC 
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG 
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA 
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT 
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC 
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA 
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG 
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG 
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA 
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC 
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA 
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG 
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG 
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG 
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA 
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG 
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA 
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT 
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC 
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT 
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC 
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT 
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG 
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC 
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC 
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA 
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG 
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT 
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC 
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC 
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT 
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG 
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC 
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC 
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG 
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG 
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG 
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG 
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG 
AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880306.1
CDS	344..2692
Translation	MDGNLAFCLHGRTWPPFSSSLFHPRKQYLEEHRFSVVVVPSPFPCALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_017024817.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024817.2

ORF Insert Sequence:

ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC 
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG 
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA 
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT 
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC 
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA 
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG 
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG 
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA 
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC 
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA 
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG 
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG 
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG 
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA 
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG 
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA 
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT 
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC 
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT 
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC 
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT 
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG 
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC 
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC 
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA 
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG 
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT 
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC 
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC 
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT 
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG 
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC 
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC 
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG 
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG 
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG 
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG 
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG 
AGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_017024818.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880307.1 Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu86965
Clone ID Related Accession (Same CDS sequence)	XM_017024818.1
Accession Version	XM_017024818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 53 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG AAATCACCCG TGATATTAAG CAATTAGATC ACGCCAAACG CCACCTGACC 
ACCTCAATCA CCACACTGAA CCACCTGCAC ATGCTGGCAG GAGGTGTCGA CTCCCTCGAA 
GCCATGACCA GGCGAAGACA ATACGGAGAA GTTGCTAATC TCCTTCAGGG TGTGATGAAT 
GTCCTGGAGC ACTTCCACAA GTATATGGGG ATTCCGCAGA TCCGGCAGCT TTCCGAAAGA 
GTGAAGGCTG CACAGACTGA GTTAGGACAG CAAATCCTGG CAGATTTTGA AGAAGCGTTT 
CCTTCCCAGG GCACCAAGAG ACCAGGAGGA CCCAGCAATG TTCTACGAGA TGCATGTCTG 
GTTGCTAATA TTCTAGATCC CAGGATCAAA CAGGAAATCA TCAAAAAGTT TATTAAACAG 
CATCTGTCAG AGTATCTGGT ACTTTTTCAA GAAAACCAAG ATGTTGCCTG GCTGGACAAA 
ATCGACAGAC GCTATGCCTG GATAAAACGC CAGCTTGTGG ACTATGAGGA GAAATACGGC 
CGCATGTTTC CACGTGAGTG GTGCATGGCT GAGAGGATTG CGGTGGAATT TTGCCATGTG 
ACAAGGGCAG AACTTGCCAA GATTATGCGT ACCAGAGCGA AGGAAATTGA AGTGAAATTG 
CTTCTTTTTG CTATTCAAAG AACAACTAAC TTTGAGGGGT TTCTTGCAAA ACGCTTCTCC 
GGCTGCACCC TGACCGATGG GACCCTGAAA AAGCTTGAGT CTCCACCCCC ATCTACCAAT 
CCCTTCCTGG AAGATGAGCC AACACCAGAG ATGGAGGAAC TGGCAACGGA GAAAGGAGAT 
TTAGATCAAC CAAAGAAGCC TAAAGCCCCA GACAATCCAT TTCATGGCAT TGTTTCCAAG 
TGTTTTGAGC CTCATCTCTA CGTGTATATC GAATCCCAAG ACAAGAACCT CGGAGAGCTG 
ATAGATCGGT TTGTGGCTGA TTTCAAAGCC CAGGGGCCAC CTAAGCCCAA CACTGATGAA 
GGGGGTGCCG TGCTCCCCAG CTGCGCCGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG 
CAATGCTCTC AGCTCAGTAC TGGGGAGCCC ATGATCGCCC TGACCACCAT TTTCCAGAAG 
TACCTCCGAG AATACGCCTG GAAAATCCTC TCTGGCAACC TGCCCAAAAC CACAACCAGC 
AGTGGAGGAC TGACTATCAG CAGCCTCCTC AAGGAAAAGG AGGGCTCAGA AGTAGCCAAG 
TTCACTCTGG AGGAGCTCTG CCTCATCTGT AACATCCTGA GCACGGCAGA GTACTGTCTG 
GCCACCACCC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TGGATGTAAG TCTGATTGAA 
CGAATCAATC TGACTGGAGA GATGGACACG TTCAGCACCG TCATCTCCAG CAGTATTCAG 
CTGCTGGTTC AGGATCTGGA TGCTGCCTGT GATCCTGCCC TGACTGCCAT GAGCAAGATG 
CAGTGGCAGA ACGTGGAGCA CGTTGGTGAC CAGAGCCCCT ACGTCACCTC TGTCATTCTG 
CACATCAAGC AGAACGTCCC CATCATCCGT GACAACCTGG CTTCCACACG CAAGTACTTC 
ACTCAGTTCT GCGTTAAATT TGCAAACTCC TTCATTCCCA AATTCATCAC CCACCTCTTC 
AAGTGCAAGC CAATTAGCAT GGTGGGAGCA GAACAGCTGC TGCTGGACAC CCACTCGCTG 
AAGATGGTCC TGCTCGATCT CCCCTCCATC AGCTCGCAGG TGGTGAGGAA GGCACCCGCC 
AGCTACACCA AGATCGTTGT CAAAGGCATG ACCCGGGCTG AGATGATCCT CAAGGTAGTG 
ATGGCCCCTC ATGAACCGTT GGTGGTGTTT GTTGACAACT ACATCAAACT TCTCACAGAC 
TGCAACACAG AAACCTTTCA GAAGATACTG GACATGAAGG GGCTGAAGAG GAGTGAGCAG 
AGCAGCATGC TGGAACTCCT GCGCCAGCGG CTCCCCGCAC CGCCCTCGGG GGCAGAAAGC 
TCCGGCTCAC TGTCCCTGAC GGCGCCAACA CCAGAGCAAG AGTCGTCACG CATCCGCAAG 
CTCGAGAAAC TCATTAAAAA GAGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880307.1
CDS	758..2887
Translation	MVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_017024818.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024818.1

ORF Insert Sequence:

ATGGTGAAAG AAATCACCCG TGATATTAAG CAATTAGATC ACGCCAAACG CCACCTGACC 
ACCTCAATCA CCACACTGAA CCACCTGCAC ATGCTGGCAG GAGGTGTCGA CTCCCTCGAA 
GCCATGACCA GGCGAAGACA ATACGGAGAA GTTGCTAATC TCCTTCAGGG TGTGATGAAT 
GTCCTGGAGC ACTTCCACAA GTATATGGGG ATTCCGCAGA TCCGGCAGCT TTCCGAAAGA 
GTGAAGGCTG CACAGACTGA GTTAGGACAG CAAATCCTGG CAGATTTTGA AGAAGCGTTT 
CCTTCCCAGG GCACCAAGAG ACCAGGAGGA CCCAGCAATG TTCTACGAGA TGCATGTCTG 
GTTGCTAATA TTCTAGATCC CAGGATCAAA CAGGAAATCA TCAAAAAGTT TATTAAACAG 
CATCTGTCAG AGTATCTGGT ACTTTTTCAA GAAAACCAAG ATGTTGCCTG GCTGGACAAA 
ATCGACAGAC GCTATGCCTG GATAAAACGC CAGCTTGTGG ACTATGAGGA GAAATACGGC 
CGCATGTTTC CACGTGAGTG GTGCATGGCT GAGAGGATTG CGGTGGAATT TTGCCATGTG 
ACAAGGGCAG AACTTGCCAA GATTATGCGT ACCAGAGCGA AGGAAATTGA AGTGAAATTG 
CTTCTTTTTG CTATTCAAAG AACAACTAAC TTTGAGGGGT TTCTTGCAAA ACGCTTCTCC 
GGCTGCACCC TGACCGATGG GACCCTGAAA AAGCTTGAGT CTCCACCCCC ATCTACCAAT 
CCCTTCCTGG AAGATGAGCC AACACCAGAG ATGGAGGAAC TGGCAACGGA GAAAGGAGAT 
TTAGATCAAC CAAAGAAGCC TAAAGCCCCA GACAATCCAT TTCATGGCAT TGTTTCCAAG 
TGTTTTGAGC CTCATCTCTA CGTGTATATC GAATCCCAAG ACAAGAACCT CGGAGAGCTG 
ATAGATCGGT TTGTGGCTGA TTTCAAAGCC CAGGGGCCAC CTAAGCCCAA CACTGATGAA 
GGGGGTGCCG TGCTCCCCAG CTGCGCCGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG 
CAATGCTCTC AGCTCAGTAC TGGGGAGCCC ATGATCGCCC TGACCACCAT TTTCCAGAAG 
TACCTCCGAG AATACGCCTG GAAAATCCTC TCTGGCAACC TGCCCAAAAC CACAACCAGC 
AGTGGAGGAC TGACTATCAG CAGCCTCCTC AAGGAAAAGG AGGGCTCAGA AGTAGCCAAG 
TTCACTCTGG AGGAGCTCTG CCTCATCTGT AACATCCTGA GCACGGCAGA GTACTGTCTG 
GCCACCACCC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TGGATGTAAG TCTGATTGAA 
CGAATCAATC TGACTGGAGA GATGGACACG TTCAGCACCG TCATCTCCAG CAGTATTCAG 
CTGCTGGTTC AGGATCTGGA TGCTGCCTGT GATCCTGCCC TGACTGCCAT GAGCAAGATG 
CAGTGGCAGA ACGTGGAGCA CGTTGGTGAC CAGAGCCCCT ACGTCACCTC TGTCATTCTG 
CACATCAAGC AGAACGTCCC CATCATCCGT GACAACCTGG CTTCCACACG CAAGTACTTC 
ACTCAGTTCT GCGTTAAATT TGCAAACTCC TTCATTCCCA AATTCATCAC CCACCTCTTC 
AAGTGCAAGC CAATTAGCAT GGTGGGAGCA GAACAGCTGC TGCTGGACAC CCACTCGCTG 
AAGATGGTCC TGCTCGATCT CCCCTCCATC AGCTCGCAGG TGGTGAGGAA GGCACCCGCC 
AGCTACACCA AGATCGTTGT CAAAGGCATG ACCCGGGCTG AGATGATCCT CAAGGTAGTG 
ATGGCCCCTC ATGAACCGTT GGTGGTGTTT GTTGACAACT ACATCAAACT TCTCACAGAC 
TGCAACACAG AAACCTTTCA GAAGATACTG GACATGAAGG GGCTGAAGAG GAGTGAGCAG 
AGCAGCATGC TGGAACTCCT GCGCCAGCGG CTCCCCGCAC CGCCCTCGGG GGCAGAAAGC 
TCCGGCTCAC TGTCCCTGAC GGCGCCAACA CCAGAGCAAG AGTCGTCACG CATCCGCAAG 
CTCGAGAAAC TCATTAAAAA GAGACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Hepatocellular carcinoma suppressor 1 promoter hypermethylation in serum. A diagnostic and prognostic study in hepatitis B.
Clinics and research in hepatology and gastroenterology41(2)171-180(2017 Mar)
Tian MM,Fan YC,Zhao J,Gao S,Zhao ZH,Chen LY,Wang K

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Nature genetics45(4)385-91, 391e1-2(2013 Apr)
Eeles RA,Olama AA,Benlloch S,Saunders EJ,Leongamornlert DA,Tymrakiewicz M,Ghoussaini M,Luccarini C,Dennis J,Jugurnauth-Little S,Dadaev T,Neal DE,Hamdy FC,Donovan JL,Muir K,Giles GG,Severi G,Wiklund F,Gronberg H,Haiman CA,Schumacher F,Henderson BE,Le Marchand L,Lindstrom S,Kraft P,Hunter DJ,Gapstur S,Chanock SJ,Berndt SI,Albanes D,Andriole G,Schleutker J,Weischer M,Canzian F,Riboli E,Key TJ,Travis RC,Campa D,Ingles SA,John EM,Hayes RB,Pharoah PD,Pashayan N,Khaw KT,Stanford JL,Ostrander EA,Signorello LB,Thibodeau SN,Schaid D,Maier C,Vogel W,Kibel AS,Cybulski C,Lubinski J,Cannon-Albright L,Brenner H,Park JY,Kaneva R,Batra J,Spurdle AB,Clements JA,Teixeira MR,Dicks E,Lee A,Dunning AM,Baynes C,Conroy D,Maranian MJ,Ahmed S,Govindasami K,Guy M,Wilkinson RA,Sawyer EJ,Morgan A,Dearnaley DP,Horwich A,Huddart RA,Khoo VS,Parker CC,Van As NJ,Woodhouse CJ,Thompson A,Dudderidge T,Ogden C,Cooper CS,Lophatananon A,Cox A,Southey MC,Hopper JL,English DR,Aly M,Adolfsson J,Xu J,Zheng SL,Yeager M,Kaaks R,Diver WR,Gaudet MM,Stern MC,Corral R,Joshi AD,Shahabi A,Wahlfors T,Tammela TL,Auvinen A,Virtamo J,Klarskov P,Nordestgaard BG,R?der MA,Nielsen SF,Bojesen SE,Siddiq A,Fitzgerald LM,Kolb S,Kwon EM,Karyadi DM,Blot WJ,Zheng W,Cai Q,McDonnell SK,Rinckleb AE,Drake B,Colditz G,Wokolorczyk D,Stephenson RA,Teerlink C,Muller H,Rothenbacher D,Sellers TA,Lin HY,Slavov C,Mitev V,Lose F,Srinivasan S,Maia S,Paulo P,Lange E,Cooney KA,Antoniou AC,Vincent D,Bacot F,Tessier DC,,,,,,Kote-Jarai Z,Easton DF

Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
Molecular biology of the cell21(19)3386-95(2010 Oct)
P?rez-Victoria FJ,Schindler C,Magad?n JG,Mardones GA,Delevoye C,Romao M,Raposo G,Bonifacino JS

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VPS53 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム