HIT1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIT1
Entrez Gene ID	841472
Full Name	Membrane trafficking VPS53 family protein
Synonyms	ARABIDOPSIS THALIANA VPS53 HOMOLOG,ATVPS53,HEAT-INTOLERANT 1,VPS53
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001333441.1	NP_001322422.1	Membrane trafficking VPS53 family protein
NM_001198259.1	NP_001185188.1	Membrane trafficking VPS53 family protein
NM_001333442.1	NP_001322423.1	Membrane trafficking VPS53 family protein
NM_001333440.1	NP_001322424.1	Membrane trafficking VPS53 family protein
NM_103933.6	NP_564573.3	Membrane trafficking VPS53 family protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VPS53	NP_001180097.1
Danio rerio (zebrafish)	vps53	NP_001007459.2
Arabidopsis thaliana (thale cress)	AT1G50970	NP_175510.2
Gallus gallus (chicken)	VPS53	NP_001012824.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006623	XP_316653.4
Caenorhabditis elegans (roundworm)	vps-53	NP_001255027.1
Magnaporthe oryzae (rice blast fungus)	MGG_01331	XP_003714256.1
Canis lupus familiaris (dog)	VPS53	XP_548308.2
Mus musculus (house mouse)	Vps53	NP_080940.2
Rattus norvegicus (Norway rat)	Vps53	NP_001099283.1
Neurospora crassa	NCU03024	XP_965410.1
Arabidopsis thaliana (thale cress)	HIT1	NP_564573.3
Xenopus tropicalis (tropical clawed frog)	LOC100496110	XP_002934389.2
Pan troglodytes (chimpanzee)	VPS53	XP_001151796.1
Schizosaccharomyces pombe (fission yeast)	vps53	NP_593341.1
Oryza sativa (rice)	Os01g0906200	NP_001045132.2
Homo sapiens (human)	VPS53	NP_001121631.1
Drosophila melanogaster (fruit fly)	CG3338	NP_608825.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The hit1-1 mutation essentially alters vesicle trafficking and results in changes in the plasma membrane components under non-stress conditions.
Title: The Arabidopsis hit1-1 mutant has a plasma membrane profile distinct from that of wild-type plants at optimal growing temperature.

HIT1 may be involved in vesicle trafficking in plant stress responses.[HIT1]
Title: Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant.

The following HIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb26010	NM_001333440.1 Latest version!	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb26010	NM_103933.6 Latest version!	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb32665	NM_001198259.1 Latest version!	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb34330	NM_001333441.1 Latest version!	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAb34331	NM_001333442.1 Latest version!	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIT1 ( NM_001333440.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322424.1 Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Price & Availability↑

CloneID	OAb26010
Clone ID Related Accession (Same CDS sequence)	NM_001333440.1 , NM_103933.6
Accession Version	NM_001333440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Membrane trafficking VPS53 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGGTTTTCAA CAGGAAAAGA CCGGAAACAA AGATTTATCT 
GTTACTGGAG CTGGGTTTAA CTTCCGTGGA ATGATCTCTT CCTGCTTTGA ACCTCATTTA 
ACTCCATATA TTGAGTTGGA AGAGAAAACA CTTATGGATG ACCTGGAGAA AATTGTTCAG 
GAGGAATCAT GGGATGTTGA GGATGGAAGC CAAAATAATG TTTTATCTAG TAGCACACAG 
CTATTTTCCA ATATAAAGAA GAGCTTGAAA CGCTGTAATA CTCTCAGTAA GAACCAGACT 
TTATTCAATT TGTTCAAGGT CTTTCAACGA GTTTTAAAGG CCTATGCTAC AAAGCTATTT 
TTTAAGCTCC CAAAAGGAGG CACTGGTATC GTTGCCGCAG CCACAGGCAT GGATGGACAG 
ATAAAGGTCT CGGAGAGAGA TGAAAGGGTG ATATGCTACA TAGTTAATTC TGCTGAGTAT 
TGCCACAAAA CATCTGGTGA ACTGGCAGAA AACGTCTCAG AAATTATTGA CCCACATTAT 
GCTGATGGTG TAGACATGTC AGAGGTCCAG GATGAATTTT CAGCCGTCAT AACGAAAGCA 
TTGGTAACGC TGGTCCTTGG ACTTGAGACT AAATTTGACA CAGAAATGGC AGTGATGACA 
CGTGTTCCAT GGAGCACACT TGAGAGTGTT GGTGACCAGT CTGGGTACGT GAATGGAATA 
AATACAGTCC TTAGCGGCAG CATACCTGTC CTCGGGAAAC TTCTAACACC AGTTTACTTC 
CAGTTTTTCC TTGACAAGCT GGCATCATCC CTAGGACCAC GGTTCTATGC TAATATTTTC 
AGATGCAAGC AACTATCCGA AACTGGAGCT CAACAGATGT TACTAGATAC GCAAGCTGTG 
AAGTCGATTC TACTAGAAAT TCCTTCCCTT GCCAGACAGA CCTCAACTGC TGCCAGCTAT 
TCCAAATTTG TGAGCCGTGA AATGAGCAGA GCTGAAGCAC TCCTTAAGGT CATACTATCC 
CCAATCGACT CAGTGGCAGA CACTTATCGT GCACTGTTTC CGGAGGGAAC GCCCATGGAG 
TTTCAACGCA TCTTAGAACT TAAGGGTCTT AAGAAAGCTG ATCAGCAGAG TATACTAGAT 
GATTTCAACA AGCATGGTCC AGGATTCACA CAGCAGTCGG TTGCAGCAGC AATGCCACAA 
CCAATGCCAA CCCCACCTGC ACCACCATTG GCAATCACTA ATCCGGCTAC AGCTGCAGGG 
TTCATAGCAA ACAGCGAGGA TGTTTTAACC AGAGCAGCTG CTCTTGGCAG AGGAGCTGCA 
AGCACTGGCT TCAAGAAGTT CATTGCTCTT ACCGAAGCTG CCAAAGACCG CAAAGATGGG 
CCTTTAAGGA GACTCTTCAA CGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322424.1
CDS	424..2910
Translation	MDKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQSVAAAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALTEAAKDRKDGPLRRLFNA

Target ORF information:

RefSeq Version	NM_001333440.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333440.1

ORF Insert Sequence:

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGGTTTTCAA CAGGAAAAGA CCGGAAACAA AGATTTATCT 
GTTACTGGAG CTGGGTTTAA CTTCCGTGGA ATGATCTCTT CCTGCTTTGA ACCTCATTTA 
ACTCCATATA TTGAGTTGGA AGAGAAAACA CTTATGGATG ACCTGGAGAA AATTGTTCAG 
GAGGAATCAT GGGATGTTGA GGATGGAAGC CAAAATAATG TTTTATCTAG TAGCACACAG 
CTATTTTCCA ATATAAAGAA GAGCTTGAAA CGCTGTAATA CTCTCAGTAA GAACCAGACT 
TTATTCAATT TGTTCAAGGT CTTTCAACGA GTTTTAAAGG CCTATGCTAC AAAGCTATTT 
TTTAAGCTCC CAAAAGGAGG CACTGGTATC GTTGCCGCAG CCACAGGCAT GGATGGACAG 
ATAAAGGTCT CGGAGAGAGA TGAAAGGGTG ATATGCTACA TAGTTAATTC TGCTGAGTAT 
TGCCACAAAA CATCTGGTGA ACTGGCAGAA AACGTCTCAG AAATTATTGA CCCACATTAT 
GCTGATGGTG TAGACATGTC AGAGGTCCAG GATGAATTTT CAGCCGTCAT AACGAAAGCA 
TTGGTAACGC TGGTCCTTGG ACTTGAGACT AAATTTGACA CAGAAATGGC AGTGATGACA 
CGTGTTCCAT GGAGCACACT TGAGAGTGTT GGTGACCAGT CTGGGTACGT GAATGGAATA 
AATACAGTCC TTAGCGGCAG CATACCTGTC CTCGGGAAAC TTCTAACACC AGTTTACTTC 
CAGTTTTTCC TTGACAAGCT GGCATCATCC CTAGGACCAC GGTTCTATGC TAATATTTTC 
AGATGCAAGC AACTATCCGA AACTGGAGCT CAACAGATGT TACTAGATAC GCAAGCTGTG 
AAGTCGATTC TACTAGAAAT TCCTTCCCTT GCCAGACAGA CCTCAACTGC TGCCAGCTAT 
TCCAAATTTG TGAGCCGTGA AATGAGCAGA GCTGAAGCAC TCCTTAAGGT CATACTATCC 
CCAATCGACT CAGTGGCAGA CACTTATCGT GCACTGTTTC CGGAGGGAAC GCCCATGGAG 
TTTCAACGCA TCTTAGAACT TAAGGGTCTT AAGAAAGCTG ATCAGCAGAG TATACTAGAT 
GATTTCAACA AGCATGGTCC AGGATTCACA CAGCAGTCGG TTGCAGCAGC AATGCCACAA 
CCAATGCCAA CCCCACCTGC ACCACCATTG GCAATCACTA ATCCGGCTAC AGCTGCAGGG 
TTCATAGCAA ACAGCGAGGA TGTTTTAACC AGAGCAGCTG CTCTTGGCAG AGGAGCTGCA 
AGCACTGGCT TCAAGAAGTT CATTGCTCTT ACCGAAGCTG CCAAAGACCG CAAAGATGGG 
CCTTTAAGGA GACTCTTCAA CGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIT1 ( NM_103933.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_564573.3 Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Price & Availability↑

CloneID	OAb26010
Clone ID Related Accession (Same CDS sequence)	NM_001333440.1 , NM_103933.6
Accession Version	NM_103933.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Membrane trafficking VPS53 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_103933.5.

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGGTTTTCAA CAGGAAAAGA CCGGAAACAA AGATTTATCT 
GTTACTGGAG CTGGGTTTAA CTTCCGTGGA ATGATCTCTT CCTGCTTTGA ACCTCATTTA 
ACTCCATATA TTGAGTTGGA AGAGAAAACA CTTATGGATG ACCTGGAGAA AATTGTTCAG 
GAGGAATCAT GGGATGTTGA GGATGGAAGC CAAAATAATG TTTTATCTAG TAGCACACAG 
CTATTTTCCA ATATAAAGAA GAGCTTGAAA CGCTGTAATA CTCTCAGTAA GAACCAGACT 
TTATTCAATT TGTTCAAGGT CTTTCAACGA GTTTTAAAGG CCTATGCTAC AAAGCTATTT 
TTTAAGCTCC CAAAAGGAGG CACTGGTATC GTTGCCGCAG CCACAGGCAT GGATGGACAG 
ATAAAGGTCT CGGAGAGAGA TGAAAGGGTG ATATGCTACA TAGTTAATTC TGCTGAGTAT 
TGCCACAAAA CATCTGGTGA ACTGGCAGAA AACGTCTCAG AAATTATTGA CCCACATTAT 
GCTGATGGTG TAGACATGTC AGAGGTCCAG GATGAATTTT CAGCCGTCAT AACGAAAGCA 
TTGGTAACGC TGGTCCTTGG ACTTGAGACT AAATTTGACA CAGAAATGGC AGTGATGACA 
CGTGTTCCAT GGAGCACACT TGAGAGTGTT GGTGACCAGT CTGGGTACGT GAATGGAATA 
AATACAGTCC TTAGCGGCAG CATACCTGTC CTCGGGAAAC TTCTAACACC AGTTTACTTC 
CAGTTTTTCC TTGACAAGCT GGCATCATCC CTAGGACCAC GGTTCTATGC TAATATTTTC 
AGATGCAAGC AACTATCCGA AACTGGAGCT CAACAGATGT TACTAGATAC GCAAGCTGTG 
AAGTCGATTC TACTAGAAAT TCCTTCCCTT GCCAGACAGA CCTCAACTGC TGCCAGCTAT 
TCCAAATTTG TGAGCCGTGA AATGAGCAGA GCTGAAGCAC TCCTTAAGGT CATACTATCC 
CCAATCGACT CAGTGGCAGA CACTTATCGT GCACTGTTTC CGGAGGGAAC GCCCATGGAG 
TTTCAACGCA TCTTAGAACT TAAGGGTCTT AAGAAAGCTG ATCAGCAGAG TATACTAGAT 
GATTTCAACA AGCATGGTCC AGGATTCACA CAGCAGTCGG TTGCAGCAGC AATGCCACAA 
CCAATGCCAA CCCCACCTGC ACCACCATTG GCAATCACTA ATCCGGCTAC AGCTGCAGGG 
TTCATAGCAA ACAGCGAGGA TGTTTTAACC AGAGCAGCTG CTCTTGGCAG AGGAGCTGCA 
AGCACTGGCT TCAAGAAGTT CATTGCTCTT ACCGAAGCTG CCAAAGACCG CAAAGATGGG 
CCTTTAAGGA GACTCTTCAA CGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_564573.3
CDS	320..2806
Translation	MDKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQSVAAAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALTEAAKDRKDGPLRRLFNA

Target ORF information:

RefSeq Version	NM_103933.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_103933.6

ORF Insert Sequence:

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGGTTTTCAA CAGGAAAAGA CCGGAAACAA AGATTTATCT 
GTTACTGGAG CTGGGTTTAA CTTCCGTGGA ATGATCTCTT CCTGCTTTGA ACCTCATTTA 
ACTCCATATA TTGAGTTGGA AGAGAAAACA CTTATGGATG ACCTGGAGAA AATTGTTCAG 
GAGGAATCAT GGGATGTTGA GGATGGAAGC CAAAATAATG TTTTATCTAG TAGCACACAG 
CTATTTTCCA ATATAAAGAA GAGCTTGAAA CGCTGTAATA CTCTCAGTAA GAACCAGACT 
TTATTCAATT TGTTCAAGGT CTTTCAACGA GTTTTAAAGG CCTATGCTAC AAAGCTATTT 
TTTAAGCTCC CAAAAGGAGG CACTGGTATC GTTGCCGCAG CCACAGGCAT GGATGGACAG 
ATAAAGGTCT CGGAGAGAGA TGAAAGGGTG ATATGCTACA TAGTTAATTC TGCTGAGTAT 
TGCCACAAAA CATCTGGTGA ACTGGCAGAA AACGTCTCAG AAATTATTGA CCCACATTAT 
GCTGATGGTG TAGACATGTC AGAGGTCCAG GATGAATTTT CAGCCGTCAT AACGAAAGCA 
TTGGTAACGC TGGTCCTTGG ACTTGAGACT AAATTTGACA CAGAAATGGC AGTGATGACA 
CGTGTTCCAT GGAGCACACT TGAGAGTGTT GGTGACCAGT CTGGGTACGT GAATGGAATA 
AATACAGTCC TTAGCGGCAG CATACCTGTC CTCGGGAAAC TTCTAACACC AGTTTACTTC 
CAGTTTTTCC TTGACAAGCT GGCATCATCC CTAGGACCAC GGTTCTATGC TAATATTTTC 
AGATGCAAGC AACTATCCGA AACTGGAGCT CAACAGATGT TACTAGATAC GCAAGCTGTG 
AAGTCGATTC TACTAGAAAT TCCTTCCCTT GCCAGACAGA CCTCAACTGC TGCCAGCTAT 
TCCAAATTTG TGAGCCGTGA AATGAGCAGA GCTGAAGCAC TCCTTAAGGT CATACTATCC 
CCAATCGACT CAGTGGCAGA CACTTATCGT GCACTGTTTC CGGAGGGAAC GCCCATGGAG 
TTTCAACGCA TCTTAGAACT TAAGGGTCTT AAGAAAGCTG ATCAGCAGAG TATACTAGAT 
GATTTCAACA AGCATGGTCC AGGATTCACA CAGCAGTCGG TTGCAGCAGC AATGCCACAA 
CCAATGCCAA CCCCACCTGC ACCACCATTG GCAATCACTA ATCCGGCTAC AGCTGCAGGG 
TTCATAGCAA ACAGCGAGGA TGTTTTAACC AGAGCAGCTG CTCTTGGCAG AGGAGCTGCA 
AGCACTGGCT TCAAGAAGTT CATTGCTCTT ACCGAAGCTG CCAAAGACCG CAAAGATGGG 
CCTTTAAGGA GACTCTTCAA CGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIT1 ( NM_001198259.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185188.1 Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Price & Availability↑

CloneID	OAb32665
Clone ID Related Accession (Same CDS sequence)	NM_001198259.1
Accession Version	NM_001198259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Membrane trafficking VPS53 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGT TTCTGCATAT 
GTGACTGCGT TTACCATGAT TTTCAAATGT GTGTTTTCCT TTACAGAGGC ATCGCTAACT 
GGCGTTGAGC CACTTATGCA AAAGATTCAG GGTGAAATTC GGCGGGTCGA TGCTAGCATA 
CTCTCTGCTG TTCGTCAACA GAGTAATTCG GGAACTAAAG CTAAAGAAGA TCTTGCTGAT 
GCTACGCGTG CTGTGGAGGA ACTCTCTCAT AAGATTCAAG AGATAAAATC CAAAGCTGAG 
CAAAGTGAAG CAATGGTTCA AGAAATATGC CGTGACATTA AGAAGTTAGA TTTTGCAAAG 
AAGAACATAA CCACGACAAT CACTGCCCTT CACCGCCTCA CAATGTTAGT CTCTGCTGTT 
GAACAGCTTC AGGTGATGGC GTCAAAAAGA CAATACAAGG AGGCAGCTGC ACAGCTCGAG 
GCTGTAAACC AATTGTGTAA CCACTTTGAA GCATACAGGG ATGTTCCTAA AATCACGGAG 
CTCAGAGAGA AGCTTAATAA TATAAAGCAA ATCCTTAAGT CCCATGTATT TTCTGATTTT 
TCCAGTTTAG GTACTGGGAA AGAGACAGAG GAAACAAATT TGCTACAGAA GTTATCTGAT 
TCTTGCTTGG TTGTTGATGC TCTGGAACCA TCTGTGAGGG AAGAGTTGGT AAATAACTTT 
TGCAGCAGGG AGCTCACCTC ATATGAACAA ATTTTTGAAG GAGCTGAATT GGCAAAGTTG 
GATAAGACAG AGCGGAGATA TGCTTGGATA AAGCGTCGAA TCCGAACAAA TGAAGAAATT 
TGGAAGATTT TTCCTGCTTC TTGGCATGTG CCGTATAGGC TATGCATTCA GTTCTGCAAG 
CAAACAAGGA AGCAAGTAGA ATCTATTCTG GTCAACATGA AAGAGAAGCC AGTTGTAGCG 
ATATTGCTTC TGGCACTACA AAGTACAGTG GAGTTTGAAA AAGAACTTGA AAAGAAATTT 
GGTGGTGGTG TTCCTACTAA AGATATTGAA GATGACATTG AAGAAATTGG TACATGGGAA 
GATAATAGTC AGAATATATC CAAAATCCGC AAGAAATATG AAAAGAAGTT TGCTGCTAGT 
CAAGAAACCG AGGAGAATGG TTTTCAACAG GAAAAGACCG GAAACAAAGA TTTATCTGTT 
ACTGGAGCTG GGTTTAACTT CCGTGGAATG ATCTCTTCCT GCTTTGAACC TCATTTAACT 
CCATATATTG AGTTGGAAGA GAAAACACTT ATGGATGACC TGGAGAAAAT TGTTCAGGAG 
GAATCATGGG ATGTTGAGGA TGGAAGCCAA AATAATGTTT TATCTAGTAG CACACAGCTA 
TTTTCCAATA TAAAGAAGAG CTTGAAACGC TGTAATACTC TCAGTAAGAA CCAGACTTTA 
TTCAATTTGT TCAAGGTCTT TCAACGAGTT TTAAAGGCCT ATGCTACAAA GCTATTTTTT 
AAGCTCCCAA AAGGAGGCAC TGGTATCGTT GCCGCAGCCA CAGGCATGGA TGGACAGATA 
AAGGTCTCGG AGAGAGATGA AAGGGTGATA TGCTACATAG TTAATTCTGC TGAGTATTGC 
CACAAAACAT CTGGTGAACT GGCAGAAAAC GTCTCAGAAA TTATTGACCC ACATTATGCT 
GATGGTGTAG ACATGTCAGA GGTCCAGGAT GAATTTTCAG CCGTCATAAC GAAAGCATTG 
GTAACGCTGG TCCTTGGACT TGAGACTAAA TTTGACACAG AAATGGCAGT GATGACACGT 
GTTCCATGGA GCACACTTGA GAGTGTTGGT GACCAGTCTG GGTACGTGAA TGGAATAAAT 
ACAGTCCTTA GCGGCAGCAT ACCTGTCCTC GGGAAACTTC TAACACCAGT TTACTTCCAG 
TTTTTCCTTG ACAAGCTGGC ATCATCCCTA GGACCACGGT TCTATGCTAA TATTTTCAGA 
TGCAAGCAAC TATCCGAAAC TGGAGCTCAA CAGATGTTAC TAGATACGCA AGCTGTGAAG 
TCGATTCTAC TAGAAATTCC TTCCCTTGCC AGACAGACCT CAACTGCTGC CAGCTATTCC 
AAATTTGTGA GCCGTGAAAT GAGCAGAGCT GAAGCACTCC TTAAGGTCAT ACTATCCCCA 
ATCGACTCAG TGGCAGACAC TTATCGTGCA CTGTTTCCGG AGGGAACGCC CATGGAGTTT 
CAACGCATCT TAGAACTTAA GGGTCTTAAG AAAGCTGATC AGCAGAGTAT ACTAGATGAT 
TTCAACAAGC ATGGTCCAGG ATTCACACAG CAGTCGGTTG CAGCAGCAAT GCCACAACCA 
ATGCCAACCC CACCTGCACC ACCATTGGCA ATCACTAATC CGGCTACAGC TGCAGGGTTC 
ATAGCAAACA GCGAGGATGT TTTAACCAGA GCAGCTGCTC TTGGCAGAGG AGCTGCAAGC 
ACTGGCTTCA AGAAGTTCAT TGCTCTTACC GAAGCTGCCA AAGACCGCAA AGATGGGCCT 
TTAAGGAGAC TCTTCAACGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185188.1
CDS	137..2680
Translation	MDKSSALEYINQMFPTVSAYVTAFTMIFKCVFSFTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQSVAAAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALTEAAKDRKDGPLRRLFNA

Target ORF information:

RefSeq Version	NM_001198259.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198259.1

ORF Insert Sequence:

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGT TTCTGCATAT 
GTGACTGCGT TTACCATGAT TTTCAAATGT GTGTTTTCCT TTACAGAGGC ATCGCTAACT 
GGCGTTGAGC CACTTATGCA AAAGATTCAG GGTGAAATTC GGCGGGTCGA TGCTAGCATA 
CTCTCTGCTG TTCGTCAACA GAGTAATTCG GGAACTAAAG CTAAAGAAGA TCTTGCTGAT 
GCTACGCGTG CTGTGGAGGA ACTCTCTCAT AAGATTCAAG AGATAAAATC CAAAGCTGAG 
CAAAGTGAAG CAATGGTTCA AGAAATATGC CGTGACATTA AGAAGTTAGA TTTTGCAAAG 
AAGAACATAA CCACGACAAT CACTGCCCTT CACCGCCTCA CAATGTTAGT CTCTGCTGTT 
GAACAGCTTC AGGTGATGGC GTCAAAAAGA CAATACAAGG AGGCAGCTGC ACAGCTCGAG 
GCTGTAAACC AATTGTGTAA CCACTTTGAA GCATACAGGG ATGTTCCTAA AATCACGGAG 
CTCAGAGAGA AGCTTAATAA TATAAAGCAA ATCCTTAAGT CCCATGTATT TTCTGATTTT 
TCCAGTTTAG GTACTGGGAA AGAGACAGAG GAAACAAATT TGCTACAGAA GTTATCTGAT 
TCTTGCTTGG TTGTTGATGC TCTGGAACCA TCTGTGAGGG AAGAGTTGGT AAATAACTTT 
TGCAGCAGGG AGCTCACCTC ATATGAACAA ATTTTTGAAG GAGCTGAATT GGCAAAGTTG 
GATAAGACAG AGCGGAGATA TGCTTGGATA AAGCGTCGAA TCCGAACAAA TGAAGAAATT 
TGGAAGATTT TTCCTGCTTC TTGGCATGTG CCGTATAGGC TATGCATTCA GTTCTGCAAG 
CAAACAAGGA AGCAAGTAGA ATCTATTCTG GTCAACATGA AAGAGAAGCC AGTTGTAGCG 
ATATTGCTTC TGGCACTACA AAGTACAGTG GAGTTTGAAA AAGAACTTGA AAAGAAATTT 
GGTGGTGGTG TTCCTACTAA AGATATTGAA GATGACATTG AAGAAATTGG TACATGGGAA 
GATAATAGTC AGAATATATC CAAAATCCGC AAGAAATATG AAAAGAAGTT TGCTGCTAGT 
CAAGAAACCG AGGAGAATGG TTTTCAACAG GAAAAGACCG GAAACAAAGA TTTATCTGTT 
ACTGGAGCTG GGTTTAACTT CCGTGGAATG ATCTCTTCCT GCTTTGAACC TCATTTAACT 
CCATATATTG AGTTGGAAGA GAAAACACTT ATGGATGACC TGGAGAAAAT TGTTCAGGAG 
GAATCATGGG ATGTTGAGGA TGGAAGCCAA AATAATGTTT TATCTAGTAG CACACAGCTA 
TTTTCCAATA TAAAGAAGAG CTTGAAACGC TGTAATACTC TCAGTAAGAA CCAGACTTTA 
TTCAATTTGT TCAAGGTCTT TCAACGAGTT TTAAAGGCCT ATGCTACAAA GCTATTTTTT 
AAGCTCCCAA AAGGAGGCAC TGGTATCGTT GCCGCAGCCA CAGGCATGGA TGGACAGATA 
AAGGTCTCGG AGAGAGATGA AAGGGTGATA TGCTACATAG TTAATTCTGC TGAGTATTGC 
CACAAAACAT CTGGTGAACT GGCAGAAAAC GTCTCAGAAA TTATTGACCC ACATTATGCT 
GATGGTGTAG ACATGTCAGA GGTCCAGGAT GAATTTTCAG CCGTCATAAC GAAAGCATTG 
GTAACGCTGG TCCTTGGACT TGAGACTAAA TTTGACACAG AAATGGCAGT GATGACACGT 
GTTCCATGGA GCACACTTGA GAGTGTTGGT GACCAGTCTG GGTACGTGAA TGGAATAAAT 
ACAGTCCTTA GCGGCAGCAT ACCTGTCCTC GGGAAACTTC TAACACCAGT TTACTTCCAG 
TTTTTCCTTG ACAAGCTGGC ATCATCCCTA GGACCACGGT TCTATGCTAA TATTTTCAGA 
TGCAAGCAAC TATCCGAAAC TGGAGCTCAA CAGATGTTAC TAGATACGCA AGCTGTGAAG 
TCGATTCTAC TAGAAATTCC TTCCCTTGCC AGACAGACCT CAACTGCTGC CAGCTATTCC 
AAATTTGTGA GCCGTGAAAT GAGCAGAGCT GAAGCACTCC TTAAGGTCAT ACTATCCCCA 
ATCGACTCAG TGGCAGACAC TTATCGTGCA CTGTTTCCGG AGGGAACGCC CATGGAGTTT 
CAACGCATCT TAGAACTTAA GGGTCTTAAG AAAGCTGATC AGCAGAGTAT ACTAGATGAT 
TTCAACAAGC ATGGTCCAGG ATTCACACAG CAGTCGGTTG CAGCAGCAAT GCCACAACCA 
ATGCCAACCC CACCTGCACC ACCATTGGCA ATCACTAATC CGGCTACAGC TGCAGGGTTC 
ATAGCAAACA GCGAGGATGT TTTAACCAGA GCAGCTGCTC TTGGCAGAGG AGCTGCAAGC 
ACTGGCTTCA AGAAGTTCAT TGCTCTTACC GAAGCTGCCA AAGACCGCAA AGATGGGCCT 
TTAAGGAGAC TCTTCAACGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIT1 ( NM_001333441.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322422.1 Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Price & Availability↑

CloneID	OAb34330
Clone ID Related Accession (Same CDS sequence)	NM_001333441.1
Accession Version	NM_001333441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Membrane trafficking VPS53 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGAAAAGACC GGAAACAAAG ATTTATCTGT TACTGGAGCT 
GGGTTTAACT TCCGTGGAAT GATCTCTTCC TGCTTTGAAC CTCATTTAAC TCCATATATT 
GAGTTGGAAG AGAAAACACT TATGGATGAC CTGGAGAAAA TTGTTCAGGA GGAATCATGG 
GATGTTGAGG ATGGAAGCCA AAATAATGTT TTATCTAGTA GCACACAGCT ATTTTCCAAT 
ATAAAGAAGA GCTTGAAACG CTGTAATACT CTCAGTAAGA ACCAGACTTT ATTCAATTTG 
TTCAAGGTCT TTCAACGAGT TTTAAAGGCC TATGCTACAA AGCTATTTTT TAAGCTCCCA 
AAAGGAGGCA CTGGTATCGT TGCCGCAGCC ACAGGCATGG ATGGACAGAT AAAGGTCTCG 
GAGAGAGATG AAAGGGTGAT ATGCTACATA GTTAATTCTG CTGAGTATTG CCACAAAACA 
TCTGGTGAAC TGGCAGAAAA CGTCTCAGAA ATTATTGACC CACATTATGC TGATGGTGTA 
GACATGTCAG AGGTCCAGGA TGAATTTTCA GCCGTCATAA CGAAAGCATT GGTAACGCTG 
GTCCTTGGAC TTGAGACTAA ATTTGACACA GAAATGGCAG TGATGACACG TGTTCCATGG 
AGCACACTTG AGAGTGTTGG TGACCAGTCT GGGTACGTGA ATGGAATAAA TACAGTCCTT 
AGCGGCAGCA TACCTGTCCT CGGGAAACTT CTAACACCAG TTTACTTCCA GTTTTTCCTT 
GACAAGCTGG CATCATCCCT AGGACCACGG TTCTATGCTA ATATTTTCAG ATGCAAGCAA 
CTATCCGAAA CTGGAGCTCA ACAGATGTTA CTAGATACGC AAGCTGTGAA GTCGATTCTA 
CTAGAAATTC CTTCCCTTGC CAGACAGACC TCAACTGCTG CCAGCTATTC CAAATTTGTG 
AGCCGTGAAA TGAGCAGAGC TGAAGCACTC CTTAAGGTCA TACTATCCCC AATCGACTCA 
GTGGCAGACA CTTATCGTGC ACTGTTTCCG GAGGGAACGC CCATGGAGTT TCAACGCATC 
TTAGAACTTA AGGGTCTTAA GAAAGCTGAT CAGCAGAGTA TACTAGATGA TTTCAACAAG 
CATGGTCCAG GATTCACACA GCAGTCGGTT GCAGCAGCAA TGCCACAACC AATGCCAACC 
CCACCTGCAC CACCATTGGC AATCACTAAT CCGGCTACAG CTGCAGGGTT CATAGCAAAC 
AGCGAGGATG TTTTAACCAG AGCAGCTGCT CTTGGCAGAG GAGCTGCAAG CACTGGCTTC 
AAGAAGTTCA TTGCTCTTAC CGAAGCTGCC AAAGACCGCA AAGATGGGCC TTTAAGGAGA 
CTCTTCAACG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322422.1
CDS	223..2697
Translation	MDKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTSTAASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGFTQQSVAAAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAALGRGAASTGFKKFIALTEAAKDRKDGPLRRLFNA

Target ORF information:

RefSeq Version	NM_001333441.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333441.1

ORF Insert Sequence:

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGAAAAGACC GGAAACAAAG ATTTATCTGT TACTGGAGCT 
GGGTTTAACT TCCGTGGAAT GATCTCTTCC TGCTTTGAAC CTCATTTAAC TCCATATATT 
GAGTTGGAAG AGAAAACACT TATGGATGAC CTGGAGAAAA TTGTTCAGGA GGAATCATGG 
GATGTTGAGG ATGGAAGCCA AAATAATGTT TTATCTAGTA GCACACAGCT ATTTTCCAAT 
ATAAAGAAGA GCTTGAAACG CTGTAATACT CTCAGTAAGA ACCAGACTTT ATTCAATTTG 
TTCAAGGTCT TTCAACGAGT TTTAAAGGCC TATGCTACAA AGCTATTTTT TAAGCTCCCA 
AAAGGAGGCA CTGGTATCGT TGCCGCAGCC ACAGGCATGG ATGGACAGAT AAAGGTCTCG 
GAGAGAGATG AAAGGGTGAT ATGCTACATA GTTAATTCTG CTGAGTATTG CCACAAAACA 
TCTGGTGAAC TGGCAGAAAA CGTCTCAGAA ATTATTGACC CACATTATGC TGATGGTGTA 
GACATGTCAG AGGTCCAGGA TGAATTTTCA GCCGTCATAA CGAAAGCATT GGTAACGCTG 
GTCCTTGGAC TTGAGACTAA ATTTGACACA GAAATGGCAG TGATGACACG TGTTCCATGG 
AGCACACTTG AGAGTGTTGG TGACCAGTCT GGGTACGTGA ATGGAATAAA TACAGTCCTT 
AGCGGCAGCA TACCTGTCCT CGGGAAACTT CTAACACCAG TTTACTTCCA GTTTTTCCTT 
GACAAGCTGG CATCATCCCT AGGACCACGG TTCTATGCTA ATATTTTCAG ATGCAAGCAA 
CTATCCGAAA CTGGAGCTCA ACAGATGTTA CTAGATACGC AAGCTGTGAA GTCGATTCTA 
CTAGAAATTC CTTCCCTTGC CAGACAGACC TCAACTGCTG CCAGCTATTC CAAATTTGTG 
AGCCGTGAAA TGAGCAGAGC TGAAGCACTC CTTAAGGTCA TACTATCCCC AATCGACTCA 
GTGGCAGACA CTTATCGTGC ACTGTTTCCG GAGGGAACGC CCATGGAGTT TCAACGCATC 
TTAGAACTTA AGGGTCTTAA GAAAGCTGAT CAGCAGAGTA TACTAGATGA TTTCAACAAG 
CATGGTCCAG GATTCACACA GCAGTCGGTT GCAGCAGCAA TGCCACAACC AATGCCAACC 
CCACCTGCAC CACCATTGGC AATCACTAAT CCGGCTACAG CTGCAGGGTT CATAGCAAAC 
AGCGAGGATG TTTTAACCAG AGCAGCTGCT CTTGGCAGAG GAGCTGCAAG CACTGGCTTC 
AAGAAGTTCA TTGCTCTTAC CGAAGCTGCC AAAGACCGCA AAGATGGGCC TTTAAGGAGA 
CTCTTCAACG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIT1 ( NM_001333442.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322423.1 Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

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CloneID	OAb34331
Clone ID Related Accession (Same CDS sequence)	NM_001333442.1
Accession Version	NM_001333442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Membrane trafficking VPS53 family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGGTTTTCAA CAGGAAAAGA CCGGAAACAA AGATTTATCT 
GTTACTGGAG CTGGGTTTAA CTTCCGTGGA ATGATCTCTT CCTGCTTTGA ACCTCATTTA 
ACTCCATATA TTGAGTTGGA AGAGAAAACA CTTATGGATG ACCTGGAGAA AATTGTTCAG 
GAGGAATCAT GGGATGTTGA GGATGGAAGC CAAAATAATG TTTTATCTAG TAGCACACAG 
CTATTTTCCA ATATAAAGAA GAGCTTGAAA CGCTGTAATA CTCTCAGTAA GAACCAGACT 
TTATTCAATT TGTTCAAGGT CTTTCAACGA GTTTTAAAGG CCTATGCTAC AAAGCTATTT 
TTTAAGCTCC CAAAAGGAGG CACTGGTATC GTTGCCGCAG CCACAGGCAT GGATGGACAG 
ATAAAGGTCT CGGAGAGAGA TGAAAGGGTG ATATGCTACA TAGTTAATTC TGCTGAGTAT 
TGCCACAAAA CATCTGGTGA ACTGGCAGAA AACGTCTCAG AAATTATTGA CCCACATTAT 
GCTGATGGTG TAGACATGTC AGAGGTCCAG GATGAATTTT CAGCCGTCAT AACGAAAGCA 
TTGGTAACGC TGGTCCTTGG ACTTGAGACT AAATTTGACA CAGAAATGGC AGTGATGACA 
CGTGTTCCAT GGAGCACACT TGAGAGTGTT GGTGACCAGT CTGGGTACGT GAATGGAATA 
AATACAGTCC TTAGCGGCAG CATACCTGTC CTCGGGAAAC TTCTAACACC AGTTTACTTC 
CAGTTTTTCC TTGACAAGCT GGCATCATCC CTAGGACCAC GGTTCTATGC TAATATTTTC 
AGATGCAAGC AACTATCCGA AACTGGAGCT CAACAGATGT TACTAGATAC GCAAGCTGTG 
AAGTCGATTC TACTAGAAAT TCCTTCCCTT GCCAGACAGC TATTCCAAAT TTGTGAGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322423.1
CDS	179..2221
Translation	MDKSSALEYINQMFPTEASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEIKSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLLQKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQLFQICEP

Target ORF information:

RefSeq Version	NM_001333442.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Membrane trafficking VPS53 family protein (HIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333442.1

ORF Insert Sequence:

ATGGATAAGT CGAGTGCTTT AGAGTATATC AACCAGATGT TCCCAACAGA GGCATCGCTA 
ACTGGCGTTG AGCCACTTAT GCAAAAGATT CAGGGTGAAA TTCGGCGGGT CGATGCTAGC 
ATACTCTCTG CTGTTCGTCA ACAGAGTAAT TCGGGAACTA AAGCTAAAGA AGATCTTGCT 
GATGCTACGC GTGCTGTGGA GGAACTCTCT CATAAGATTC AAGAGATAAA ATCCAAAGCT 
GAGCAAAGTG AAGCAATGGT TCAAGAAATA TGCCGTGACA TTAAGAAGTT AGATTTTGCA 
AAGAAGAACA TAACCACGAC AATCACTGCC CTTCACCGCC TCACAATGTT AGTCTCTGCT 
GTTGAACAGC TTCAGGTGAT GGCGTCAAAA AGACAATACA AGGAGGCAGC TGCACAGCTC 
GAGGCTGTAA ACCAATTGTG TAACCACTTT GAAGCATACA GGGATGTTCC TAAAATCACG 
GAGCTCAGAG AGAAGCTTAA TAATATAAAG CAAATCCTTA AGTCCCATGT ATTTTCTGAT 
TTTTCCAGTT TAGGTACTGG GAAAGAGACA GAGGAAACAA ATTTGCTACA GAAGTTATCT 
GATTCTTGCT TGGTTGTTGA TGCTCTGGAA CCATCTGTGA GGGAAGAGTT GGTAAATAAC 
TTTTGCAGCA GGGAGCTCAC CTCATATGAA CAAATTTTTG AAGGAGCTGA ATTGGCAAAG 
TTGGATAAGA CAGAGCGGAG ATATGCTTGG ATAAAGCGTC GAATCCGAAC AAATGAAGAA 
ATTTGGAAGA TTTTTCCTGC TTCTTGGCAT GTGCCGTATA GGCTATGCAT TCAGTTCTGC 
AAGCAAACAA GGAAGCAAGT AGAATCTATT CTGGTCAACA TGAAAGAGAA GCCAGTTGTA 
GCGATATTGC TTCTGGCACT ACAAAGTACA GTGGAGTTTG AAAAAGAACT TGAAAAGAAA 
TTTGGTGGTG GTGTTCCTAC TAAAGATATT GAAGATGACA TTGAAGAAAT TGGTACATGG 
GAAGATAATA GTCAGAATAT ATCCAAAATC CGCAAGAAAT ATGAAAAGAA GTTTGCTGCT 
AGTCAAGAAA CCGAGGAGAA TGGTTTTCAA CAGGAAAAGA CCGGAAACAA AGATTTATCT 
GTTACTGGAG CTGGGTTTAA CTTCCGTGGA ATGATCTCTT CCTGCTTTGA ACCTCATTTA 
ACTCCATATA TTGAGTTGGA AGAGAAAACA CTTATGGATG ACCTGGAGAA AATTGTTCAG 
GAGGAATCAT GGGATGTTGA GGATGGAAGC CAAAATAATG TTTTATCTAG TAGCACACAG 
CTATTTTCCA ATATAAAGAA GAGCTTGAAA CGCTGTAATA CTCTCAGTAA GAACCAGACT 
TTATTCAATT TGTTCAAGGT CTTTCAACGA GTTTTAAAGG CCTATGCTAC AAAGCTATTT 
TTTAAGCTCC CAAAAGGAGG CACTGGTATC GTTGCCGCAG CCACAGGCAT GGATGGACAG 
ATAAAGGTCT CGGAGAGAGA TGAAAGGGTG ATATGCTACA TAGTTAATTC TGCTGAGTAT 
TGCCACAAAA CATCTGGTGA ACTGGCAGAA AACGTCTCAG AAATTATTGA CCCACATTAT 
GCTGATGGTG TAGACATGTC AGAGGTCCAG GATGAATTTT CAGCCGTCAT AACGAAAGCA 
TTGGTAACGC TGGTCCTTGG ACTTGAGACT AAATTTGACA CAGAAATGGC AGTGATGACA 
CGTGTTCCAT GGAGCACACT TGAGAGTGTT GGTGACCAGT CTGGGTACGT GAATGGAATA 
AATACAGTCC TTAGCGGCAG CATACCTGTC CTCGGGAAAC TTCTAACACC AGTTTACTTC 
CAGTTTTTCC TTGACAAGCT GGCATCATCC CTAGGACCAC GGTTCTATGC TAATATTTTC 
AGATGCAAGC AACTATCCGA AACTGGAGCT CAACAGATGT TACTAGATAC GCAAGCTGTG 
AAGTCGATTC TACTAGAAAT TCCTTCCCTT GCCAGACAGC TATTCCAAAT TTGTGAGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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HIT1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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