Chd8 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006519476.2	XP_006519539.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_006519476.3	XP_006519539.1	chromodomain-helicase-DNA-binding protein 8 isoform X2
XM_017316171.2	XP_017171660.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_017316172.2	XP_017171661.1	chromodomain-helicase-DNA-binding protein 8 isoform X2
XM_017316171.1	XP_017171660.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
NM_201637.3	NP_963999.2	chromodomain-helicase-DNA-binding protein 8
XM_011245165.2	XP_011243467.2	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_017316170.1	XP_017171659.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_017316170.2	XP_017171659.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_017316172.1	XP_017171661.1	chromodomain-helicase-DNA-binding protein 8 isoform X3
NM_201637.2	NP_963999.2	chromodomain-helicase-DNA-binding protein 8
XM_011245165.3	XP_011243467.2	chromodomain-helicase-DNA-binding protein 8 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78841	XM_011245165.3 Latest version!	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu78841	XM_017316171.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu78841	XM_017316170.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu78842	XM_017316172.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu09622	NM_201637.3 Latest version!	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu09622	XM_006519476.3 Latest version!	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OMu09622	XM_006519476.2	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu78841	XM_017316171.1	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu78841	XM_017316170.1	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu78842	XM_017316172.1	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu09622	NM_201637.2	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu78841	XM_011245165.2	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

Chd8 ( XM_017316171.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171660.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu78841
Clone ID Related Accession (Same CDS sequence)	XM_017316171.2 , XM_017316171.1 , XM_011245165.2 , XM_017316170.1 , XM_017316170.2 , XM_011245165.3
Accession Version	XM_017316171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171660.1
CDS	495..8267
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_017316171.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316171.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_017316171.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171660.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X3, mRNA.

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CloneID	OMu78841
Clone ID Related Accession (Same CDS sequence)	XM_017316171.2 , XM_017316171.1 , XM_011245165.2 , XM_017316170.1 , XM_017316170.2 , XM_011245165.3
Accession Version	XM_017316171.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171660.1
CDS	493..8265
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_017316171.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316171.1

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_011245165.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243467.2 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.

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CloneID	OMu78841
Clone ID Related Accession (Same CDS sequence)	XM_017316171.2 , XM_017316171.1 , XM_011245165.2 , XM_017316170.1 , XM_017316170.2 , XM_011245165.3
Accession Version	XM_011245165.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243467.2
CDS	346..8118
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_011245165.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245165.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_017316170.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171659.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X2, mRNA.

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CloneID	OMu78841
Clone ID Related Accession (Same CDS sequence)	XM_017316171.2 , XM_017316171.1 , XM_011245165.2 , XM_017316170.1 , XM_017316170.2 , XM_011245165.3
Accession Version	XM_017316170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171659.1
CDS	891..8663
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_017316170.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316170.1

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_017316170.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171659.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X2, mRNA.

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CloneID	OMu78841
Clone ID Related Accession (Same CDS sequence)	XM_017316171.2 , XM_017316171.1 , XM_011245165.2 , XM_017316170.1 , XM_017316170.2 , XM_011245165.3
Accession Version	XM_017316170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171659.1
CDS	440..8212
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_017316170.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316170.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_011245165.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243467.2 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.

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CloneID	OMu78841
Clone ID Related Accession (Same CDS sequence)	XM_017316171.2 , XM_017316171.1 , XM_011245165.2 , XM_017316170.1 , XM_017316170.2 , XM_011245165.3
Accession Version	XM_011245165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243467.2
CDS	582..8354
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_011245165.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245165.3

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGCTGGA GGTGGAGTGC ATGGAAGAGC CTAATCACCT TGATCTGGAC 
CTGGAGACCC GGATCCCTGT CATCAATAAG GTGGATGGTA CTTTGCTGGT GGGTGATGAG 
GCCCCTCGCC GGGCGGAGCT GGAGATGTGG TTACAGGGTC ATCCAGAGTT TGCTGTTGAT 
CCCCGATTTC TAGCGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_017316172.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171661.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X4, mRNA.

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CloneID	OMu78842
Clone ID Related Accession (Same CDS sequence)	XM_017316172.2 , XM_017316172.1
Accession Version	XM_017316172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171661.1
CDS	582..8174
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVEYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_017316172.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316172.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_017316172.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171661.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X5, mRNA.

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CloneID	OMu78842
Clone ID Related Accession (Same CDS sequence)	XM_017316172.2 , XM_017316172.1
Accession Version	XM_017316172.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171661.1
CDS	347..7939
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSVFRVSPFPGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVEYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_017316172.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316172.1

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGTCTT CCGTGTCTCC CCATTCCCAG GTCTGTCTAT CCCTGTGCCC 
CGTGGGCGTA AGGGGAAAAA AGTCAAGTCA CAAAGTACTT TTGACATTCA TAAGGCAGAC 
TGGATCCGGA AATACAACCC TGATACTCTG TTCCAAGATG AGAGTTATAA GAAGCATTTG 
AAACATCAGT GTAACAAGGT ACTGTTGAGA GTCCGGATGC TCTACTACCT GAGACAGGAG 
GTTATTGGAG ACCAGGCAGA GAAGGTGTTA GGAGGTGCCA TAGCCAGTGA GATTGACATA 
TGGTTCCCAG TGGTGGATCA GCTGGAGGTT CCTACAACTT GGTGGGATAG TGAGGCTGAC 
AAATCCCTGC TCATTGGCGT TTTTAAGCAT GGCTATGAGA AATACAATAC CATGAGGGCA 
GACCCTGCCT TGTGCTTCCT AGAAAAGGCT GGCAGGCCAG ATGACAAAGC CATCGCAGCA 
GAACATAGAG TGTTGGATAA TTTCTCCGAC CTGGTAGAAG GGATTGACTT TGATAAGGAT 
TGTGAAGATC CTGAATATAA ACCCCTCCAG GGTCCTCCAA AGGACCCAGA TGATGAGGGT 
GATCCCTTGA TGATGATGGA TGAGGAGATC TCAGTCATCG ACGGAGAGGA AGCCCAGGTA 
ACTCAACAGC CAGGGCATTT ATTCTGGCCT CCAGGCTCCG CCCTCACAGC TAGGCTTCGC 
CGCCTAGTAA CAGCCTATCA ACGCAGCTAC AAGAGAGAAC AAATGAAGAT GGAGGCTGCG 
GAACGTGGGG ACCGGAGACG CCGACGATGT GAAGCAGCCT TTAAGCTCAA AGAAATCGCA 
AGGCGGGAAA AACAACAACG GTGGACAAGG CGTGAGCAAA CTGATTTCTA CCGAGTAGTA 
TCTACCTTTG GTGTGGAGTA TGACCCTGAC AACATGCAGT TTCACTGGGA TCGCTTCCGC 
ACTTTTGCTC GCCTGGACAA AAAAACAGAT GAAAGCCTTA CCAAGTACTT CCATGGTTTT 
GTGGCCATGT GTCGACAAGT GTGCCGCCTT CCCCCAGCTG CTGGTGATGA ACCTCCAGAC 
CCTAATCTGT TCATTGAGCC CATCACTGAA GAGAGAGCCT CGAGGACTCT CTACCGAATT 
GAGTTGCTTC GACGCTTACG GGAACAAGTT TTATGCCACC CCCTTTTAGA AGATCGGCTG 
GCATTGTGTC AGCCTCCAGG TCTTGAATTG CCCAAATGGT GGGAACCTGT CCGTCATGAT 
GGGGAGCTCC TACGAGGAGC AGCCCGCCAT GGGGTGAGCC AAACAGACTG CAACATCATG 
CAGGACCCAG ACTTCTCTTT TCTGGCTGCC CGTATGAATT ATATGCAGAA CCATCAGGCG 
GGAGCATCAG CTGCATCCCT GTCGCGATGC TCCACTCCAC TGCTGCACCA GCAGTGTACC 
TCACGTACTG CTTCACCATC ACCCCTGCGC CCAGATGCTC CTGTTGAAAA GTCACCTGAG 
GAAAGTACTG TTCAGGTCCC CAATCTGGAG AGTCTGACTT TAAAGTTAGA GGATGAGGTT 
GTGGCTAGGA GCCGACTCAC CTCACAAGAC TATGAAGTAC GCGTAGGCTC TTCAGATACA 
GCTCCTCTGT CCCGGAGTGT CCCACCAGTG AAACTGGAGG ACGAGGATGA TTCAGACTCT 
GAGCTGGACT TGAGCAAGTT GTCACCATCC TCCTCCTCTT CTTCATCCTC GTCCAGCTCC 
AGCTCCAGCA CTGACGAGAG TGAAGACGAG AAGGAAGAGA AGTTAACTGC TGACCGGTCC 
CGCCCAAAGC TCTATGATGA AGAGAGCCTT CTGTCCCTTA CTATGTCCCA AGATGGATTC 
CCAAATGAAG ATGGAGAACA AATGACCCCT GAGTTGCTGC TACTGCAGGA AAGACAAAGA 
GCCTCTGAAT GGCCTAAGGA TCGTGTTCTG ATAAACCGCA TTGACCTCGT CTGCCAGGCT 
GTACTCTCAG GGAAATGGCC TTCTAACCGC CGGAGCCAGG AAGTGACAGC AGGAGGAATT 
TTGGGGCCAG GCAACCATTT ATTAGACAGT CCCTCTTTGA CCCCAGGAGA AGATGGGGAC 
TCTCCAGTCC CCACGCCACG AAGTGGCAGT GCAGCTTCCA TGGCGGAGGA AGAGGCATCT 
GCAGTCACCA CAGCGGCGGC CCAGTTTACG AAACTTCGGC GAGGCATGGA TGAGAAAGAG 
TTTACAGTTC AGATCAAAGA TGAAGAAGGA TTGAAGTTAA CATTCCAGAA GCATAGATTG 
ATGGCTAATG GTGTAATGGG AGATGGACAT CCACTGTTTC ATAAGAAGAA GGGGAACAGG 
AAAAAGCTAG TAGAGTATAT GGAGGAACGT AGAAAACAGA AGTGGCAGAG ATGTAAAAAA 
AATAATAAGG CAGAATTGAA CTGTTTGGGA ATGGAACCAG TACAGCCAGC GAACTCTAGA 
AATGGGAAAA AGGGTCACTA TGCTGAAACT GCGTTCAACC GGGTTTTGCC AGGGCCTGTT 
GCACCAGAGA ACAGCAAGAA ACGGGTCCGT AGGACAAGAC CAGACCTTTC TAAGATGATG 
GCCCTGATGC AGGGTGGAAG CACTGGGTCC CTGTCTCTGC ATAACACCTT TCAACACAGC 
AGTAGTAACC TACAGTCTGT GTCGTCTCTG GGTCACAGCA GTACCACTTC TGCATCTCTG 
CCTTTTATGC CGTTTGTGAT GGGTGCAGCA GCACCCCCTC ATGTAGACTC CAGCACCATG 
CTTCATCATC ACCACCACCA TCCCCACCCC CACCATCACC ACCATCACCA TCCAGGCTTG 
AGAACCACTG GCTACCCTTC TTCACCAGCT ACTACCACCT CTGGTACTGC CTTGCGGTTA 
CCAACACTGC AACCTGAGGA CGATGATGAA GAGGAGGATG AAGAAGATGA TGATTTATCT 
CAGGGCTATG ACAGCTCAGA ACGGGACTTC TCACTCATTG ATGACCCTAT GATGCCAGCC 
AACTCAGACT CCAGTGAAGA TGCTGACGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( NM_201637.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963999.2 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), mRNA.

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CloneID	OMu09622
Clone ID Related Accession (Same CDS sequence)	XM_006519476.2 , XM_006519476.3 , NM_201637.3 , NM_201637.2
Accession Version	NM_201637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126037.4 and AC159323.3. On or before Jun 20, 2019 this sequence version replaced XM_006519476.3, NM_201637.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ190419.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963999.2
CDS	300..8048
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	NM_201637.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201637.3

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( NM_201637.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963999.2 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), mRNA.

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CloneID	OMu09622
Clone ID Related Accession (Same CDS sequence)	XM_006519476.2 , XM_006519476.3 , NM_201637.3 , NM_201637.2
Accession Version	NM_201637.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ190419.1. On or before Dec 15, 2006 this sequence version replaced XM_619244.3, XM_907992.2, XM_987619.1, NM_201637.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ190419.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963999.2
CDS	1..7749
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	NM_201637.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201637.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_006519476.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519539.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.

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CloneID	OMu09622
Clone ID Related Accession (Same CDS sequence)	XM_006519476.2 , XM_006519476.3 , NM_201637.3 , NM_201637.2
Accession Version	XM_006519476.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006519539.1

Translation

MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_006519476.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519476.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd8 ( XM_006519476.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519539.1 Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X4, mRNA.

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CloneID	OMu09622
Clone ID Related Accession (Same CDS sequence)	XM_006519476.2 , XM_006519476.3 , NM_201637.3 , NM_201637.2
Accession Version	XM_006519476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519539.1
CDS	346..8094
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	XM_006519476.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 8 (Chd8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519476.3

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTTTTTGAT GACCCAAACT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAATCA GGTCACTCAA GACCCCATTG AAGAAGCACT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAAGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGACCTGGT TCCTCCACCA GAGGAAACAG CTTCCACTGA ACTTCCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCTTA ACCTTGCATG ATTATACCAC TCAGCCCACC 
AGCCAGGAGC AGCCAGCCCA GCCTGTCTTG CAGACATCAA CACCAACAGC AGGACTCTTG 
CAGGTCTCCA AGAGCCAGGA GATCTTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGGTGTCT CCCCCAGTAA TACTGGAGGA CAACCATCTC AGTCAGCTCC TAAGATTGTT 
ATTCTTAAAG CTCCACCAAA CTCCTCCGTC ACAGGCACCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGTACAGC TCAGCCCCTA GTGGCTGGGA CAGCCAACGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTGAC TGGTACACCC CTTCGACCAG GTGTATCCAT TGTCTCTGGT 
AATACAGTGT TGGCCACGAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCAAGCCGAC CAGTAAAACA GCTGGTCCTC CAGCCAGTAA AGGGTTCAGC TCCTGCTGGG 
AATCCTGGGG CTGCCGGGCC CCCACTGAAG CCTGCAGTTA CACTGACGTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACTTTGGTC CTTCAACAGC CACAGTCCGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGTT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTAGCAGCCT TGACTCAAGC CAAGAATGCC CAGGGGCAGC CTGCCAAAGT AGTAACTATT 
CAGCTGCAGG TGCAGCAGCC ACAGCAGAAA ATCCAGATTG TACCTCAGCC TCCATCATCA 
CAACCACAGC CACAGCCGCA GCCGCCACCC TCAGCCCAGC CGTTGACCCT GTCCTCTGTG 
CAGCAGGCTC AGATAATGGG GCCAGGCCAA AACCCAGGGC AGAGACTTTC TGTACCGCTC 
AAGATGGTGC TGCAGCCACA GGCTGGCTCT TCTCAGGGAG CCTCTTCTGG ACTCTCTGTG 
GTTAAAGTTC TAAGTGCCAG TGAAGTGGCA GCCCTGTCAT CCCCAGCAAG CTGTGCCCCT 
CATACTGCAG GGAAGACTGG GATGGAGGAG AACCGCAGGT TGGAGCACCA GAAAAAGCAA 
GAGAAAGCAA ATCGGATTGT GGCAGAGGCC ATTGCTAGGG CCCGGGCCAG GGGCGAGCAA 
AACATACCTC GAGTCCTGAA TGAGGATGAG CTGCCTAGTG TCCGGCCAGA GGAGGAGGGT 
GAAAAGAAAC GCAGGAAGAA GAGCAGTGGG GAAAGGCTGA AGGAGGAGAA GCCAAAGAAG 
AGCAAGACTG CTGCTGCCTC CAAAACGAAG GGCAAGAGTA AGCTAAACAC CATCACTCCT 
GTAGTGGGCA AGAAGAGAAA GCGTAACACA TCGTCTGACA ACTCTGACGT GGAAGTTATG 
CCTGCACAAT CGCCCCGGGA GGACGAAGAG AGCAGCATCC AGAAGAGACG TTCAAACCGC 
CAAGTTAAGC GAAAAAAATA TACAGAGGAC CTGGATATAA AGATAACAGA CGATGAAGAG 
GAGGAGGAGG TCGATGTAAC TGGTCCAATA AAACCCGAGC CCATCCTCCC AGAACCAGTG 
CAAGAGCCTG ATGGCGAGAC TCTTCCTTCC ATGCAGTTCT TTGTGGAGAA TCCTAGTGAA 
GAAGATGCAG CTATTGTGGA CAAAGTGCTT TCCATGCGAG TTGTGAAGAA AGAGCTTCCT 
TCTGGACAAT ACACTGAGGC TGAAGAATTC TTCGTCAAGT ACAAGAACTA CTCTTATCTG 
CACTGTGAGT GGGCAACGAT CTCCCAGCTG GAGAAGGATA AGAGGATCCA CCAGAAACTA 
AAACGGTTCA AAACAAAAAT GGCTCAGATG AGACACTTCT TTCATGAAGA TGAAGAGCCT 
TTCAATCCAG ACTACGTAGA GGTGGATAGG ATACTGGACG AGTCTCACAG TGTTGACAAG 
GATAATGGTG AGCCTGTAAT TTACTACCTG GTAAAATGGT GCTCTCTGCC CTATGAGGAC 
AGTACGTGGG AGCTAAAAGA GGATGTTGAT GAGGGCAAGA TTCGGGAATT TAAACGGATC 
CAGTCAAGGC ACCCAGAACT GAGAAGGGTG AATCGTCCAC AGGCAAATGC CTGGAAGAAA 
TTGGAGTTAT CACATGAATA TAAAAACAGA AACCAATTAC GGGAATATCA GTTAGAAGGG 
GTCAACTGGC TCCTCTTTAA TTGGTATAAC AGGCAGAACT GCATCCTGGC TGATGAAATG 
GGATTGGGCA AAACTATTCA GTCAATCGCC TTCTTGCAGG AGGTATATAA TGTAGGCATC 
CATGGTCCCT TTTTGGTCAT TGCTCCATTG TCCACCATTA CTAACTGGGA GCGTGAATTC 
AATACATGGA CAGAGATGAA CACTATTGTG TACCATGGCA GCCTGGCCAG CCGGCAGATG 
ATCCAGCAGT ATGAAATGTA CTGTAAAGAC TCACGGGGTC GCCTCATCCC TGGTGCATAT 
AAGTTTGATG CCTTGATCAC CACTTTTGAG ATGATTTTGT CAGACTGTCC TGAGCTTCGT 
GAAATTGAAT GGCGTTGTGT TATCATTGAT GAAGCCCATA GGCTGAAGAA CCGTAATTGC 
AAGCTACTTG ATAGTCTCAA GCACATGGAC CTGGAGCATA AAGTGTTACT CACAGGGACA 
CCGTTACAAA ATACCGTAGA GGAACTGTTC AGTCTACTGC ATTTCTTGGA ACCTTCTCAG 
TTCCCCTCAG AATCAGAATT CCTTAAGGAT TTTGGGGATC TCAAGACCGA GGAGCAGGTT 
CAAAAGCTTC AGGCCATTCT AAAGCCAATG ATGCTGAGGA GACTCAAAGA GGATGTTGAA 
AAAAATTTGG CTCCCAAACA GGAAACTATT ATTGAAGTAG AGCTAACCAA CATCCAGAAG 
AAATACTACA GAGCTATTTT AGAGAAGAAT TTCTCCTTCC TTTCCAAAGG GGCAGGTCAT 
ACCAATATGC CTAATCTACT GAACACAATG ATGGAGCTAC GAAAGTGCTG CAATCACCCG 
TACCTCATCA ATGGTGCAGA AGAGAAAATC CTGATGGAAT TTCGGGAAGC TTGCCATATT 
ATACCTCAAG ATTTCCACCT GCAGGCTATG GTTCGGTCAG CTGGCAAGTT AGTTCTCATT 
GACAAGTTAC TTCCAAAGCT TAAAGCTGGT GGCCATAAAG TTCTGATCTT CTCCCAGATG 
GTACGCTGTC TAGACATTCT GGAGGATTAT CTGATCCAGA GGAGGTACTT ATATGAACGT 
ATTGATGGGC GAGTTAGAGG CAACCTTCGA CAAGCTGCTA TCGACCGCTT TAGCAAGCCT 
GACTCAGACC GCTTTGTCTT CTTGCTATGT ACCCGTGCTG GTGGACTTGG TATTAATCTT 
ACAGCTGCTG ATACCTGTAT TATCTTTGAT TCAGACTGGA ATCCACAGAA TGACCTGCAG 
GCCCAAGCAC GTTGTCATCG AATTGGACAG AGCAAAGCTG TGAAGGTGTA CCGACTCATC 
ACTCGTAATT CCTATGAGAG AGAGATGTTT GATAAAGCTA GCCTCAAGTT GGGATTGGAT 
AAAGCTGTGC TTCAGTCCAT GAGTGGTCGG GATGGCAACA TTACTGGAAT TCAACAGTTC 
TCCAAGAAAG AGATTGAAGA TCTCTTAAGG AAAGGTGCCT ATGCGGCCAT AATGGAGGAA 
GACGATGAGG GTTCTAAGTT TTGTGAGGAG GACATAGATC AGATCTTACT GAGACGGACC 
ACAACCATCA CTATTGAGTC TGAAGGGAAA GGTTCTACTT TTGCCAAGGC AAGCTTTGTT 
GCTTCTGAAA ATAGGACAGA TATTTCCCTG GATGATCCAA ACTTTTGGCA AAAATGGGCC 
AAAAAGGCAG ACCTAGACAT GGATCTGCTG AATAGTAAGA ATAACTTAGT GATCGACACA 
CCTCGAGTTC GGAAACAGAC TCGACACTTC AGCACTTTGA AAGATGATGA CCTGGTTGAG 
TTTTCTGATT TGGAAAGTGA AGATGATGAA CGGCCACGTT CCCGAAGACA TGACCGGCAT 
CACACCTATG GGCGCACTGA CTGCTTCCGG GTAGAAAAGC ATCTCCTGGT ATATGGTTGG 
GGACGTTGGA GAGATATTTT GTCTCACGGA CGATTTAAGC GGCGCATGAC TGAAAGAGAT 
GTTGAGACAA TATGCCGTGC CATCCTCGTG TACTGTCTTC TACACTACCG TGGGGATGAA 
AACATCAAAA GCTTCATTTG GGACTTGATT AGCCCTGCTG AAAATGGCAA GACAAAAGAA 
TTGCAGAATC ATTCAGGTCT GTCTATCCCT GTGCCCCGTG GGCGTAAGGG GAAAAAAGTC 
AAGTCACAAA GTACTTTTGA CATTCATAAG GCAGACTGGA TCCGGAAATA CAACCCTGAT 
ACTCTGTTCC AAGATGAGAG TTATAAGAAG CATTTGAAAC ATCAGTGTAA CAAGGTACTG 
TTGAGAGTCC GGATGCTCTA CTACCTGAGA CAGGAGGTTA TTGGAGACCA GGCAGAGAAG 
GTGTTAGGAG GTGCCATAGC CAGTGAGATT GACATATGGT TCCCAGTGGT GGATCAGCTG 
GAGGTTCCTA CAACTTGGTG GGATAGTGAG GCTGACAAAT CCCTGCTCAT TGGCGTTTTT 
AAGCATGGCT ATGAGAAATA CAATACCATG AGGGCAGACC CTGCCTTGTG CTTCCTAGAA 
AAGGCTGGCA GGCCAGATGA CAAAGCCATC GCAGCAGAAC ATAGAGTGTT GGATAATTTC 
TCCGACCTGG TAGAAGGGAT TGACTTTGAT AAGGATTGTG AAGATCCTGA ATATAAACCC 
CTCCAGGGTC CTCCAAAGGA CCCAGATGAT GAGGGTGATC CCTTGATGAT GATGGATGAG 
GAGATCTCAG TCATCGACGG AGAGGAAGCC CAGGTAACTC AACAGCCAGG GCATTTATTC 
TGGCCTCCAG GCTCCGCCCT CACAGCTAGG CTTCGCCGCC TAGTAACAGC CTATCAACGC 
AGCTACAAGA GAGAACAAAT GAAGATGGAG GCTGCGGAAC GTGGGGACCG GAGACGCCGA 
CGATGTGAAG CAGCCTTTAA GCTCAAAGAA ATCGCAAGGC GGGAAAAACA ACAACGGTGG 
ACAAGGCGTG AGCAAACTGA TTTCTACCGA GTAGTATCTA CCTTTGGTGT GGAGTATGAC 
CCTGACAACA TGCAGTTTCA CTGGGATCGC TTCCGCACTT TTGCTCGCCT GGACAAAAAA 
ACAGATGAAA GCCTTACCAA GTACTTCCAT GGTTTTGTGG CCATGTGTCG ACAAGTGTGC 
CGCCTTCCCC CAGCTGCTGG TGATGAACCT CCAGACCCTA ATCTGTTCAT TGAGCCCATC 
ACTGAAGAGA GAGCCTCGAG GACTCTCTAC CGAATTGAGT TGCTTCGACG CTTACGGGAA 
CAAGTTTTAT GCCACCCCCT TTTAGAAGAT CGGCTGGCAT TGTGTCAGCC TCCAGGTCTT 
GAATTGCCCA AATGGTGGGA ACCTGTCCGT CATGATGGGG AGCTCCTACG AGGAGCAGCC 
CGCCATGGGG TGAGCCAAAC AGACTGCAAC ATCATGCAGG ACCCAGACTT CTCTTTTCTG 
GCTGCCCGTA TGAATTATAT GCAGAACCAT CAGGCGGGAG CATCAGCTGC ATCCCTGTCG 
CGATGCTCCA CTCCACTGCT GCACCAGCAG TGTACCTCAC GTACTGCTTC ACCATCACCC 
CTGCGCCCAG ATGCTCCTGT TGAAAAGTCA CCTGAGGAAA GTACTGTTCA GGTCCCCAAT 
CTGGAGAGTC TGACTTTAAA GTTAGAGGAT GAGGTTGTGG CTAGGAGCCG ACTCACCTCA 
CAAGACTATG AAGTACGCGT AGGCTCTTCA GATACAGCTC CTCTGTCCCG GAGTGTCCCA 
CCAGTGAAAC TGGAGGACGA GGATGATTCA GACTCTGAGC TGGACTTGAG CAAGTTGTCA 
CCATCCTCCT CCTCTTCTTC ATCCTCGTCC AGCTCCAGCT CCAGCACTGA CGAGAGTGAA 
GACGAGAAGG AAGAGAAGTT AACTGCTGAC CGGTCCCGCC CAAAGCTCTA TGATGAAGAG 
AGCCTTCTGT CCCTTACTAT GTCCCAAGAT GGATTCCCAA ATGAAGATGG AGAACAAATG 
ACCCCTGAGT TGCTGCTACT GCAGGAAAGA CAAAGAGCCT CTGAATGGCC TAAGGATCGT 
GTTCTGATAA ACCGCATTGA CCTCGTCTGC CAGGCTGTAC TCTCAGGGAA ATGGCCTTCT 
AACCGCCGGA GCCAGGAAGT GACAGCAGGA GGAATTTTGG GGCCAGGCAA CCATTTATTA 
GACAGTCCCT CTTTGACCCC AGGAGAAGAT GGGGACTCTC CAGTCCCCAC GCCACGAAGT 
GGCAGTGCAG CTTCCATGGC GGAGGAAGAG GCATCTGCAG TCACCACAGC GGCGGCCCAG 
TTTACGAAAC TTCGGCGAGG CATGGATGAG AAAGAGTTTA CAGTTCAGAT CAAAGATGAA 
GAAGGATTGA AGTTAACATT CCAGAAGCAT AGATTGATGG CTAATGGTGT AATGGGAGAT 
GGACATCCAC TGTTTCATAA GAAGAAGGGG AACAGGAAAA AGCTAGTAGA GCTGGAGGTG 
GAGTGCATGG AAGAGCCTAA TCACCTTGAT CTGGACCTGG AGACCCGGAT CCCTGTCATC 
AATAAGGTGG ATGGTACTTT GCTGGTGGGT GATGAGGCCC CTCGCCGGGC GGAGCTGGAG 
ATGTGGTTAC AGGGTCATCC AGAGTTTGCT GTTGATCCCC GATTTCTAGC GTATATGGAG 
GAACGTAGAA AACAGAAGTG GCAGAGATGT AAAAAAAATA ATAAGGCAGA ATTGAACTGT 
TTGGGAATGG AACCAGTACA GCCAGCGAAC TCTAGAAATG GGAAAAAGGG TCACTATGCT 
GAAACTGCGT TCAACCGGGT TTTGCCAGGG CCTGTTGCAC CAGAGAACAG CAAGAAACGG 
GTCCGTAGGA CAAGACCAGA CCTTTCTAAG ATGATGGCCC TGATGCAGGG TGGAAGCACT 
GGGTCCCTGT CTCTGCATAA CACCTTTCAA CACAGCAGTA GTAACCTACA GTCTGTGTCG 
TCTCTGGGTC ACAGCAGTAC CACTTCTGCA TCTCTGCCTT TTATGCCGTT TGTGATGGGT 
GCAGCAGCAC CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCATCACCA CCACCATCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAA CCACTGGCTA CCCTTCTTCA 
CCAGCTACTA CCACCTCTGG TACTGCCTTG CGGTTACCAA CACTGCAACC TGAGGACGAT 
GATGAAGAGG AGGATGAAGA AGATGATGAT TTATCTCAGG GCTATGACAG CTCAGAACGG 
GACTTCTCAC TCATTGATGA CCCTATGATG CCAGCCAACT CAGACTCCAG TGAAGATGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Chd8
Entrez Gene ID	67772
Full Name	chromodomain helicase DNA binding protein 8
Synonyms	5830451P18Rik,AU015341,Duplin,HELSNF1,mKIAA1564
General protein information	Preferred Names chromodomain helicase DNA binding protein 8 Names chromodomain-helicase-DNA-binding protein 8 ATP-dependent helicase CHD8 CHD-8 axis duplication inhibitor helicase with SNF2 domain 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C2
Summary	This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which is common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. In mammals, this gene has been shown to function in several processes including transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Knockout of this gene causes early embryonic lethality due to widespread apoptosis. Heterozygous loss of function mutations result in autism spectrum disorder-like behaviors that include increased anxiety, repetitive behavior, and altered social behavior. [provided by RefSeq, Dec 2016].

	CHD8	XP_001096619.2
Bos taurus (cattle)	CHD8	NP_001179063.1
Danio rerio (zebrafish)	chd8	NP_001189381.1
Canis lupus familiaris (dog)	CHD8	XP_532624.4
Rattus norvegicus (Norway rat)	Chd8	NP_075222.2
Pan troglodytes (chimpanzee)	CHD8	XP_003314292.1
Mus musculus (house mouse)	Chd8	NP_963999.2
Homo sapiens (human)	CHD8	NP_001164100.1
Xenopus tropicalis (tropical clawed frog)	chd8	NP_001131089.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Chd8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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