CHD8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD8
Entrez Gene ID	568214
Full Name	chromodomain helicase DNA binding protein 8
Synonyms	CHD-8,si:ch211-10e2.6,wu:fi45h08
General protein information	Preferred Names chromodomain helicase DNA binding protein 8 Names chromodomain-helicase-DNA-binding protein 8 ATP-dependent helicase CHD8 chromodomain helicase DNA binding protein family
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)
Summary	This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which is common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. In mammals, this gene has been shown to function in several processes including transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Morpholino knockdown of this gene in zebrafish results in macrocephaly that is likely caused by abnormal cell proliferation during development. [provided by RefSeq, Dec 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_021481014.1	XP_021336689.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
NM_001347671.1	NP_001334600.1	chromodomain-helicase-DNA-binding protein 8
XM_005171313.3	XP_005171370.1	chromodomain-helicase-DNA-binding protein 8 isoform X2

Pathways from BioSystems

Homologies

	CHD8	XP_001096619.2
Bos taurus (cattle)	CHD8	NP_001179063.1
Danio rerio (zebrafish)	chd8	NP_001189381.1
Canis lupus familiaris (dog)	CHD8	XP_532624.4
Rattus norvegicus (Norway rat)	Chd8	NP_075222.2
Pan troglodytes (chimpanzee)	CHD8	XP_003314292.1
Mus musculus (house mouse)	Chd8	NP_963999.2
Homo sapiens (human)	CHD8	NP_001164100.1
Xenopus tropicalis (tropical clawed frog)	chd8	NP_001131089.2

Gene Ontology
Bibliography

Related articles in PubMed

CHD8short, a naturally-occurring truncated form of a chromatin remodeler lacking the helicase domain, is a potent transcriptional coregulator.
Kunkel GR, Tracy JA, Jalufka FL, Lekven AC
Gene641303-309(2018 Jan)

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Asad Z, Pandey A, Babu A, Sun Y, Shevade K, Kapoor S, Ullah I, Ranjan S, Scaria V, Bajpai R, Sachidanandan C
Human molecular genetics25(16)3539-3554(2016 08)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Characterization of ?? T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity.
Wan F, Hu CB, Ma JX, Gao K, Xiang LX, Shao JZ
Frontiers in immunology7675(2016)

CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors.
Sugathan A, Biagioli M, Golzio C, Erdin S, Blumenthal I, Manavalan P, Ragavendran A, Brand H, Lucente D, Miles J, Sheridan SD, Stortchevoi A, Kellis M, Haggarty SJ, Katsanis N, Gusella JF, Talkowski ME
Proceedings of the National Academy of Sciences of the United States of America111(42)E4468-77(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHD8 precipitates a network of gene-expression changes involved in neurodevelopmental pathways in which many autism spectrum disorder-associated genes may converge on shared mechanisms of pathogenesis
Title: CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors.

The following CHD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa07225	NM_001347671.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 8 (chd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa07226	XM_005171313.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa07226	XM_021481014.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD8 ( NM_001347671.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001334600.1 Danio rerio chromodomain helicase DNA binding protein 8 (chd8), mRNA.

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CloneID	ODa07225
Clone ID Related Accession (Same CDS sequence)	NM_001347671.1
Accession Version	NM_001347671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928674.5. On Dec 6, 2016 this sequence version replaced XM_005171312.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGACC CTATTATGGA CCTCTTTGAT GACACACCAC TGTTCAATTT GGACTCCCTG 
CCGGAGGATG CGTTCTCCCA GGGTTCCTCA GACCCAGTAG AGGAGGCGCT GAAGTTGGCA 
TTGGGCCAAG TAGACCCTCC TACAGACCCC ATACCTGACC CTGGTGTTCC TATTCTGAGT 
GACGTGGTCA CAGATCCAGC TCTAATCCCA ACTCCTGTCT CTGTTCCACT GCAAAACCTT 
CAAACTCAGC AGCTCAGCCA GATCCCCCAT GAGGTCTCTG TTGCCTCTGC TCCCATTTCC 
ATTCAGCCCT CCCTCTCGGT GGCCAGTAAC AGCAGTGGTG CTGCAACAGT CTTGCTGAGC 
TCTTCTCTTG GTGTACCTGT GTCAGGGGCT CAAGTCACCC CTCAGCAGCA GACTCAGCAA 
ATCACTGCTG TTACGCAACA GGCAGCGGGA CAGCACGCCC CAAAAATTGT CATTCTGAAA 
GGCCCGCAGG GTCAGACTCA AGTGCTGCAG GGTGTCACAG GAGCCACGGG CTCTCCAGGG 
AAAGTCACCC TCGCTAGAGT TCTGACAGGG ACCCCTCTTA GGCCCGGCAT GGCAGTTGTT 
TCTGGAGGGA CGGTATTAAA TGCGACATCT CCTGCGCAGG GGCAGGTCAA AGTTGGCACT 
GGAGTACAAA GGCTGGTTCA AACTGCAAAT GGCCCAATGA AACAGGTGTT ACTGACCTCA 
GTGCCCCAGA CGCAATCGCA GGTGCAGACA CAGCCTGTCC AGGTGCAAAT ACCTGTGCAG 
ACACAGCTGC AGTCACCATC GCAGCCGCAA CAGCTTCAGG CGCAGATCCA GGCTCAGACA 
CAGGTGGCAT TACAAACACA AGCCCAAACG CAGACTCCTA CTTCTCCGGC AGCAGCAGGC 
ATCCGACCTC AAAGTGTCAC ACTTTCTGCA GTACCACAGC AGGGGGAAGC AAAGAGGATC 
ACTCTGGTGC TACAGCAGCC CTCACAAGGT GGAGCTGCCC AAGCTGGGAC GGTGACAGTA 
GGGGGAACTG CAGGGTCGGG TCAGCAAGGC CAGCAGCCCA GGCTGGTGTT GGGCTCTCTG 
CCAGGGAAGT TAGTCCTGCA GGGAGACCAG CTGGCAGCAC TCGCCCAAGC CAAGGCAGGT 
CAAACGGGTG CACAGGCAAA AGTGCTCACT ATTCAGCTGC AGGTTCAACA GCAGCCCAAC 
CAACAGGGAG CCAAGTTTCA GTTGGTTTCT GGGGCAGCAA ATGCTGGTGG CAGTCCACAG 
GTGGTGCAGA TTTCTCAAGG CCAAGGAGGA CAAAGACTAG CAGTGCCCCT TAAACTACTG 
CTGCAGCCAC AGTCAAACAC TGTTTCCAGC GCTGGTGGAG CTGTCTCTGT GGTTAAAGTT 
ATCAATACCT CAGCTGCTGG CTCGACGTCC GGCACCACCA CTACAGCTGC ATCTTCCGGG 
GTACGCTTGG CAAAGATCCA GGAGCCCGTA CGGAGAGTGG AAACGCTTTG CAAGCAAGAG 
AAAGCAAATC GCATTGTTGC TGAGGCCATC GCACGGGCCA AAGCCAGGGG TGAAAGGAAC 
ATCCCACGAG TGCTCAACCA GGATGAACTA CCTGCCGGAC AAACCTCAGC AGACCTAGAG 
GGCGCAGGAG GGGCTACCGG AGCCAAGAAG AAAGGAGGAG GAGGAGTTGG AGGTGGTGGA 
GGTGGAAGCA AGAAGAAAAG CCCAAGTGCA GGAGGAGCGA AAATGGTGGT GGGAGGAGAT 
AAGAAATCCA AGGCAAAAAC TCCAGTTATA CCTGGTGGAG GCAGTAAAAG CAAATCTAAG 
ACTAAGCTCA ACACCATCAC TCTAGTGGGT AAGAAAAGGA AAAGAAACCC GTCTTCTGAT 
CACTCAGACG TAGATCTCAG CCCACCTGTT TCGCCCCGCA CACTGGAGGA AGAAATGTCA 
CAGAAGAGAC GTTCAAACCG GCAAGTGAAA CGGAAGAAGT ACACTGAGGA TTTGGACATT 
AAGATCACAG ATGATGAAGA TGAATTGGAT GCAGATGTTG ATGTGACCAC AACTCCTATG 
CCCGCAGTAG GGCATGTCCA GCCTTTGGGA GCAGAGCTCC CCCCGGAGCT GGATGGGGAT 
GGTCTTCCAA GCATGCAGTT TTTTGTGGAG AACCCAAGTG AGGAGGATGC TGCTATTGTG 
GATAAAATAT TGTCTATGCG GGTGACCAAA AAGGAGGTGT GTCCAGGGCA GTATACTAAT 
GTAGAGGAGT TTTTTGTCAA ATATAAAAAC TATTCGTACA TGCACTGTGA ATGGGCCAGT 
CTTGAGCAGC TGGAAAGAGA CAAGAGGATC CATCAGAAGC TTAAGAGATT CAAGACGAAA 
CAGGCACAAA TGAGGAACCT GTTCCAAGAG GATGAGGAAC CTTTCAATCC AGATTATGTG 
GAAGTTGATC GTATTTTGGA CGAGTCACAT AGTGTTGATA AAGACAACGG GGAGCCAGTT 
GTGTACTACC TGGTGAAGTG GTGCTCTCTA CCCTATGAAG ATGCTACTTG GGAGCTGAAG 
GAGGATGTTG ATGAAGGCAA AGTGGAAGAA TTCAGGAAGA TTGAAAGCCG CCAACCACGA 
CTCAAGAGAA CTCCTCGGCC TGCTGCAAGT GCCTGGAAAA AGCTAGATGA GTCCACCGAG 
TATAAGAATG GGAACCAGCT CAGAGAATAC CAGCTTGAGG GAGTCAACTG GCTGCTCTTT 
AACTGGTACA ACAGGCAAAA CTGCATCCTA GCGGATGAAA TGGGTCTGGG GAAGACAATT 
CAGTCCATTG CCCTGCTGTC AGAGATGTTT TCTGCGGGGG TCCAGAGCCC GTTCATGATC 
ATTGCCCCTC TGTCCACGAT CACAAACTGG GAAAGAGAGT TTTCGAACTG GACTGATATG 
AATGCTATTG TTTATCACGG CAGTCTGGCT AGTAGACAGA TGATCCAGCA GTATGAAATG 
TACTGCAAAG ATGACAAGGG ACACTTGATA CCAGGGGCCT ATAAGTTTGA CGCTCTGATC 
ACAACCTTTG AGATGATTCT ATCAGACTGC CCAGAGCTGA GGGAGATTTC CTGGCGCTGT 
GTAGTTATTG ATGAAGCTCA CCGTTTGAAG AACAGAAACT GCAAACTCCT CGACAGTCTC 
AAGATGCTTG AAATTGAGCA TAAAGTGTTG CTTACTGGCA CTCCACTACA AAACACAGTA 
GAGGAGCTCT TCAGTCTGCT GCACTTTCTG GAACCGGCCC AGTTTCCCTC AGAGATAGAG 
TTCTTGCGAG AGTTTGGAGA TCTTAAAACT GAGGAGCAGG TGCAGAAACT ACAGTCAATC 
CTGAAGCCCA TGATGCTGCG CAGACTGAAG GAAGATGTAG AAAAGAACCT TGCCCCCAAG 
CAAGAAACTA TTATTGAGGT GGAGTTGACC GATGTACAGA AAAAGTATTA CCGTGCTATT 
CTGGAGAGAA ACTTCAGCTT CCTTAGCATG GGAGCCACAC AAAACAGCAA TGTACCCAAT 
CTCCTCAACA CAATGATGGA ACTTCGAAAG TGCTGCAACC ACCCATACCT CATTACAGGT 
GCTGAGGAGA AGATCGTGTC TGAGCTGAGG GAGGTGTATG ATCCTCTGGC TCCGGACTTT 
CATCTCCAGG CTCTGGTGCG CTCTGCAGGG AAACTGGTCT TGTTGGATAA GCTGCTTCCT 
CGCCTCAAGG CTGGCGGGCA CAAGGTGCTC ATCTTCTCCC AGATGGTGAG GTGCCTTGAC 
ATCCTTGAGG ACTACCTCAT ACACAAGAGA TACCTGTATG AGCGCATTGA TGGACGGGTC 
AGAGGAAACC TGCGACAGGC GGCCATTGAT CGCTTCAGTA AACCTGATTC AGACCGGTTT 
GTCTTCCTGC TTTGTACTCG GGCTGGAGGT TTGGGCATTA ATCTGACCGC TGCTGACACC 
TGTGTCATTT TTGACTCTGA CTGGAACCCG CAGAATGATC TTCAAGCTCA GGCACGTTGC 
CATCGTATTG GTCAGTCTAA AGCGGTAAAG GTGTATCGTC TCATTACCAG AAACTCCTAC 
GAGAGGGAGA TGTTGGACAA AGCTAGTCTT AAACTGGGGC TTGACAGAGC CGTTCTGCAG 
AGCATGAGTG GAAACAAGGA AAGCAGTGTC AACGGGATCC AGCAGTTCTC TAAGAAGGAG 
ATTGAGGATT TGCTTCGTAA AGGTGCTTAT GCAGCCATAA TGGATGAGAA TGACGAGGGC 
AGTCGTTTCT GTGAAGAGGA CATCGACCAG ATCCTTCAGA GACGAGCAAC TACCATCACA 
ATAGAGAGCG AAGGCAAAGG ATCCACTTTT TCCAAGGCTA GCTTTGTGGC GTCTGAGAAC 
CGCACAGATA TCGCTCTAGA TGATCCCGAG TTCTGGCAGA AGTGGGCTAA GAAAGCAGAC 
ATAGACATGG ATTCTCTCAA CAGGAAGAAT ACTCTTGTCA TCGACACACC AAGAGTAAGA 
AAGCAAACAC GTCAGTTTTC CAGCCTGCGT GGAGAGGGTG GTGATCTCTC TGACTTGGAC 
AGCGATGATG ACTACCCACC CCATAATTCC CGTCAATCCA GAGCCTCCAG ACGCTCTGAC 
CGGCATTCTG GAGGAGGCTA TGGTCGCACA GACTGTTTCC GAGTGGAGAA ACATCTACTC 
GTTTATGGGT GGGGTCGTTG GCGAGACATC CTGTCGCATG CTCGCTGTAA GCGGCGTCTC 
AGTGAACGGG ACGTAGAAAC CATCTGCCGT GTCATCCTGG TTTTCTGTCT GATTCATTAT 
CGTGGAGATG AGAACATCAA GAGCTTCATT TGGGAGCTGA TTACCCCTCC AGAGAATGGA 
CGAGAACCAC AAGCTCTGCT CAATCACTCT GGTCTGTCCA TCCCTGTTCC TCGCGGCCGA 
AAGGGAAAGA GAGTGAAGGC TCAGAGCTCT TTTGACGTGC AGAAGGTTGA GTGGATCCGC 
AAATACAATC CTGACAGTCT GCTGCTGGAT GACAGTTACA GAAAACACCT CAAACACCAG 
TGCAACAAGG TTTTGCTGAG AGTACGTATG CTTTATTATC TGAAGCAGGA GGTCATTGGA 
GAACATGCTG ATTCTGTATT GAGTGGAGCT GATGCAAGGG ATATTGATAT CTGGTTGCCT 
GAAATGGAGC AGCAGGATGT TCCATCTGGC TGGTGGGATG CAGAAGCTGA CCGATGTTTG 
CTTATCGGAG TCTACAAGCA TGGATATGAG ATGTACACCA CCATGCGTGC AGACCCCTGC 
TTGTGTTTTG TTGAGCGATG TGGTCGTCCA AATGAGCAGG ACATTAATGC AGAGCAGCAA 
GCAGCAGACC CAGAGCTTGG AGAGGGAGGG GACTACGACA AGTACTCTGA AGATCCAGAG 
TTCAAACCCG CAACGAGACA TGCCAAGGAG ATGTATGAGG AGGGTGATTC GGTGAATGCA 
GATGGTGAGA TTTGTGTGGA GGATCGATCT GCCCCTATGC AAGTTGAAGG ACCGTCTTCG 
GGATCATCTG ATTTGTGTTA CTGGCCCACC AGCTCATCGC TCACAGCCCG ACTGCGACGT 
CTTATCACAG CTTACCAGCG CAGTTACAGA CGAGAGCAGC TTAAGATCGA GGCAGCAGAG 
AAGGGGGACC GTAGGCGAAG ACGCTGCGAA CAAGCTACCA AACTCAAAGA GATAGCCAGA 
CAAGAGCGAC AGCAACGGTG GACTCGTAGA GAAGAGTGCG ACTTTTACAG GGTGGTATCG 
ACATTTGGGG TGGAAAGGAT AAAGAAAGAA ACAGATGCTC CAGAAGGGGA CGAGCATCAC 
ATGGACTGGA ATCGTTTCCG TTCTTTTGCT CGACTCGATA AGAAAACCGA TGAGAGCTTG 
ACACGTTACT TTAAGTGCTT CATGTCCATG TGTCGGAAAG TGTGTCACAT TCGGCCAGGA 
CGTGGAGACG AATCTCAAGA TATGTCTCAG TCTCTGGCCC CGATCACAGA AGAGCGAGCC 
TCACGAACTC TCTACAGGGT CACCCTGCTG TGTCGGTTGC GTGAGCGTGT TCTTCCTCAC 
CCCTCGCTCG AGGAACGCCT CAGTCTGGCA CCACAAACCT CTGACCTGCC CAGTTGGTGG 
AGCATCCCAA AGCATGACCA CGAGCTTCTC TTAGCTGCTG CCAGACATGG AGTGAGCCGC 
ACTGAACTCT CCATCTTCTC TGACCCACTG TACTCCTTCA GTCAGAGCCG CCTCGACTAC 
CTCCAGAACC AGCAGGCTCA AGCAGCTGCA CAAATCCACG CCTTCAGTCA GTCACAGGAT 
CCAGCCGGCA TCAAAGAAGA GGGCCTGGAG GATGAGTCTC GACTGCTGGG TGTGGAGGCT 
CTCTGTCCGT CAGACTCTCC GGCCATGCTG CTCTCTCATT CTGACAGCAA AGTTGGCATT 
CAGGCTGGCT GGGTCTGGAA GAAGAGCAAA AACAACGGGC CGAGTGAGAG GAAATTGGGA 
GGGGGAGGAG GAGGGGCTTC AGACTCTGAT TCTGATTCAG ATTCAGGCTC GTCTTCCTCC 
TCTCGGCATT CAGGCAGCTC AGATGATAGT GGAGACAGTG ATGTGGAGAG AGAACAAGCA 
GCAGCTCTTA AGATGTGTGA TGGTGATGAA GAAAACAGCA TCCTCTCACT CACGCCGTCA 
CAGGAAGGAG CTCCACCTGA GTCCCTCACT GACCCTCTAA GAGTGGACTG GCCCAAAGAC 
CGCATTCTGA TCAACAGGAT AGAAAACCTC TGTTCACTGG TTATTACGGG ACACTGGCCG 
TCAGGTCGCC GCTACATCTC TGACATCCAA CTTAACACTG TATCTGATGA GCATGAACTC 
GGAGATGACC TCGGTTACTC ACGGGTGGCA CGAAAAATCA ACAGTACCTT ATCTGCAGAG 
GCTCTCGAAG GCCAGGAGTC AGAGTTTACA GTAAAGCTCC TAAAGGAGGA AGGCCTAAAG 
CTGACCTTCT CCAAGCAGGC TCTGATGCCA AACGGTGAAG GGAGTGCACG CAAAAAGCGA 
AAGGACCATG AGCTGGAAGA TGCAGAAGGG GTTCTCCACG CTCCCCGCAG GAGGGACCTT 
CCCAACTGGC TCAAAGAAAA CCCAGATTAT GAGGTTGAGG GAGATATGCT AGAATTACTG 
GTGAACCGGA CCAAAAGGAA GAGGAGGAGG AAGAGGGTGG AGAAAGGAGC AGCACTTACT 
GGCAGCGAGA GAGTGAAGGT CATAGACATA CGGACAGGCA AGAAGTTTGC TGGAGTGTTC 
GGGCCTGCAC TACAGGACCT GAGAGAGCAC CTGGAGGAGA ATCCTGACCA CGCTGTAGCA 
CCGGAATGGT CTGAGACTGT TCGCCACTCG GGCTTCTTGC CTGAGATTTT ATTCCACAGA 
CTGTTGAGTC CCCACGCTTC AATCCCCAAA AAGAGCCGGC ATTACCTCCA CACCCCTTCC 
CTTCAGACCG ACGACCCTCT CCTGGGAGGC GGTGAAGGTG AGATGCTAGT TTCTGACGGC 
GCCTACATGA TGGACGATGA AGACCTGGAG GACGGAGGTC ACCTCACGTC ATCTCACCAC 
TTCCTCACTC CAGCCTATGA TGTGAAAATG GAACCGAGCG CGCTCGATAT GGACGGAGGC 
GACAGTTTGT CACAGGGCGG TTATGATAGC TCTGACAGAG AGGCCATTTT GGATGATGTC 
ATCATGGCAC CCAAACATTC GGACACTTCC TCGAGCTCAG AGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334600.1
CDS	348..8015
Translation	MADPIMDLFDDTPLFNLDSLPEDAFSQGSSDPVEEALKLALGQVDPPTDPIPDPGVPILSDVVTDPALIPTPVSVPLQNLQTQQLSQIPHEVSVASAPISIQPSLSVASNSSGAATVLLSSSLGVPVSGAQVTPQQQTQQITAVTQQAAGQHAPKIVILKGPQGQTQVLQGVTGATGSPGKVTLARVLTGTPLRPGMAVVSGGTVLNATSPAQGQVKVGTGVQRLVQTANGPMKQVLLTSVPQTQSQVQTQPVQVQIPVQTQLQSPSQPQQLQAQIQAQTQVALQTQAQTQTPTSPAAAGIRPQSVTLSAVPQQGEAKRITLVLQQPSQGGAAQAGTVTVGGTAGSGQQGQQPRLVLGSLPGKLVLQGDQLAALAQAKAGQTGAQAKVLTIQLQVQQQPNQQGAKFQLVSGAANAGGSPQVVQISQGQGGQRLAVPLKLLLQPQSNTVSSAGGAVSVVKVINTSAAGSTSGTTTTAASSGVRLAKIQEPVRRVETLCKQEKANRIVAEAIARAKARGERNIPRVLNQDELPAGQTSADLEGAGGATGAKKKGGGGVGGGGGGSKKKSPSAGGAKMVVGGDKKSKAKTPVIPGGGSKSKSKTKLNTITLVGKKRKRNPSSDHSDVDLSPPVSPRTLEEEMSQKRRSNRQVKRKKYTEDLDIKITDDEDELDADVDVTTTPMPAVGHVQPLGAELPPELDGDGLPSMQFFVENPSEEDAAIVDKILSMRVTKKEVCPGQYTNVEEFFVKYKNYSYMHCEWASLEQLERDKRIHQKLKRFKTKQAQMRNLFQEDEEPFNPDYVEVDRILDESHSVDKDNGEPVVYYLVKWCSLPYEDATWELKEDVDEGKVEEFRKIESRQPRLKRTPRPAASAWKKLDESTEYKNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIALLSEMFSAGVQSPFMIIAPLSTITNWEREFSNWTDMNAIVYHGSLASRQMIQQYEMYCKDDKGHLIPGAYKFDALITTFEMILSDCPELREISWRCVVIDEAHRLKNRNCKLLDSLKMLEIEHKVLLTGTPLQNTVEELFSLLHFLEPAQFPSEIEFLREFGDLKTEEQVQKLQSILKPMMLRRLKEDVEKNLAPKQETIIEVELTDVQKKYYRAILERNFSFLSMGATQNSNVPNLLNTMMELRKCCNHPYLITGAEEKIVSELREVYDPLAPDFHLQALVRSAGKLVLLDKLLPRLKAGGHKVLIFSQMVRCLDILEDYLIHKRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCVIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMLDKASLKLGLDRAVLQSMSGNKESSVNGIQQFSKKEIEDLLRKGAYAAIMDENDEGSRFCEEDIDQILQRRATTITIESEGKGSTFSKASFVASENRTDIALDDPEFWQKWAKKADIDMDSLNRKNTLVIDTPRVRKQTRQFSSLRGEGGDLSDLDSDDDYPPHNSRQSRASRRSDRHSGGGYGRTDCFRVEKHLLVYGWGRWRDILSHARCKRRLSERDVETICRVILVFCLIHYRGDENIKSFIWELITPPENGREPQALLNHSGLSIPVPRGRKGKRVKAQSSFDVQKVEWIRKYNPDSLLLDDSYRKHLKHQCNKVLLRVRMLYYLKQEVIGEHADSVLSGADARDIDIWLPEMEQQDVPSGWWDAEADRCLLIGVYKHGYEMYTTMRADPCLCFVERCGRPNEQDINAEQQAADPELGEGGDYDKYSEDPEFKPATRHAKEMYEEGDSVNADGEICVEDRSAPMQVEGPSSGSSDLCYWPTSSSLTARLRRLITAYQRSYRREQLKIEAAEKGDRRRRRCEQATKLKEIARQERQQRWTRREECDFYRVVSTFGVERIKKETDAPEGDEHHMDWNRFRSFARLDKKTDESLTRYFKCFMSMCRKVCHIRPGRGDESQDMSQSLAPITEERASRTLYRVTLLCRLRERVLPHPSLEERLSLAPQTSDLPSWWSIPKHDHELLLAAARHGVSRTELSIFSDPLYSFSQSRLDYLQNQQAQAAAQIHAFSQSQDPAGIKEEGLEDESRLLGVEALCPSDSPAMLLSHSDSKVGIQAGWVWKKSKNNGPSERKLGGGGGGASDSDSDSDSGSSSSSRHSGSSDDSGDSDVEREQAAALKMCDGDEENSILSLTPSQEGAPPESLTDPLRVDWPKDRILINRIENLCSLVITGHWPSGRRYISDIQLNTVSDEHELGDDLGYSRVARKINSTLSAEALEGQESEFTVKLLKEEGLKLTFSKQALMPNGEGSARKKRKDHELEDAEGVLHAPRRRDLPNWLKENPDYEVEGDMLELLVNRTKRKRRRKRVEKGAALTGSERVKVIDIRTGKKFAGVFGPALQDLREHLEENPDHAVAPEWSETVRHSGFLPEILFHRLLSPHASIPKKSRHYLHTPSLQTDDPLLGGGEGEMLVSDGAYMMDDEDLEDGGHLTSSHHFLTPAYDVKMEPSALDMDGGDSLSQGGYDSSDREAILDDVIMAPKHSDTSSSSED

Target ORF information:

RefSeq Version	NM_001347671.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 8 (chd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347671.1

ORF Insert Sequence:

ATGGCAGACC CTATTATGGA CCTCTTTGAT GACACACCAC TGTTCAATTT GGACTCCCTG 
CCGGAGGATG CGTTCTCCCA GGGTTCCTCA GACCCAGTAG AGGAGGCGCT GAAGTTGGCA 
TTGGGCCAAG TAGACCCTCC TACAGACCCC ATACCTGACC CTGGTGTTCC TATTCTGAGT 
GACGTGGTCA CAGATCCAGC TCTAATCCCA ACTCCTGTCT CTGTTCCACT GCAAAACCTT 
CAAACTCAGC AGCTCAGCCA GATCCCCCAT GAGGTCTCTG TTGCCTCTGC TCCCATTTCC 
ATTCAGCCCT CCCTCTCGGT GGCCAGTAAC AGCAGTGGTG CTGCAACAGT CTTGCTGAGC 
TCTTCTCTTG GTGTACCTGT GTCAGGGGCT CAAGTCACCC CTCAGCAGCA GACTCAGCAA 
ATCACTGCTG TTACGCAACA GGCAGCGGGA CAGCACGCCC CAAAAATTGT CATTCTGAAA 
GGCCCGCAGG GTCAGACTCA AGTGCTGCAG GGTGTCACAG GAGCCACGGG CTCTCCAGGG 
AAAGTCACCC TCGCTAGAGT TCTGACAGGG ACCCCTCTTA GGCCCGGCAT GGCAGTTGTT 
TCTGGAGGGA CGGTATTAAA TGCGACATCT CCTGCGCAGG GGCAGGTCAA AGTTGGCACT 
GGAGTACAAA GGCTGGTTCA AACTGCAAAT GGCCCAATGA AACAGGTGTT ACTGACCTCA 
GTGCCCCAGA CGCAATCGCA GGTGCAGACA CAGCCTGTCC AGGTGCAAAT ACCTGTGCAG 
ACACAGCTGC AGTCACCATC GCAGCCGCAA CAGCTTCAGG CGCAGATCCA GGCTCAGACA 
CAGGTGGCAT TACAAACACA AGCCCAAACG CAGACTCCTA CTTCTCCGGC AGCAGCAGGC 
ATCCGACCTC AAAGTGTCAC ACTTTCTGCA GTACCACAGC AGGGGGAAGC AAAGAGGATC 
ACTCTGGTGC TACAGCAGCC CTCACAAGGT GGAGCTGCCC AAGCTGGGAC GGTGACAGTA 
GGGGGAACTG CAGGGTCGGG TCAGCAAGGC CAGCAGCCCA GGCTGGTGTT GGGCTCTCTG 
CCAGGGAAGT TAGTCCTGCA GGGAGACCAG CTGGCAGCAC TCGCCCAAGC CAAGGCAGGT 
CAAACGGGTG CACAGGCAAA AGTGCTCACT ATTCAGCTGC AGGTTCAACA GCAGCCCAAC 
CAACAGGGAG CCAAGTTTCA GTTGGTTTCT GGGGCAGCAA ATGCTGGTGG CAGTCCACAG 
GTGGTGCAGA TTTCTCAAGG CCAAGGAGGA CAAAGACTAG CAGTGCCCCT TAAACTACTG 
CTGCAGCCAC AGTCAAACAC TGTTTCCAGC GCTGGTGGAG CTGTCTCTGT GGTTAAAGTT 
ATCAATACCT CAGCTGCTGG CTCGACGTCC GGCACCACCA CTACAGCTGC ATCTTCCGGG 
GTACGCTTGG CAAAGATCCA GGAGCCCGTA CGGAGAGTGG AAACGCTTTG CAAGCAAGAG 
AAAGCAAATC GCATTGTTGC TGAGGCCATC GCACGGGCCA AAGCCAGGGG TGAAAGGAAC 
ATCCCACGAG TGCTCAACCA GGATGAACTA CCTGCCGGAC AAACCTCAGC AGACCTAGAG 
GGCGCAGGAG GGGCTACCGG AGCCAAGAAG AAAGGAGGAG GAGGAGTTGG AGGTGGTGGA 
GGTGGAAGCA AGAAGAAAAG CCCAAGTGCA GGAGGAGCGA AAATGGTGGT GGGAGGAGAT 
AAGAAATCCA AGGCAAAAAC TCCAGTTATA CCTGGTGGAG GCAGTAAAAG CAAATCTAAG 
ACTAAGCTCA ACACCATCAC TCTAGTGGGT AAGAAAAGGA AAAGAAACCC GTCTTCTGAT 
CACTCAGACG TAGATCTCAG CCCACCTGTT TCGCCCCGCA CACTGGAGGA AGAAATGTCA 
CAGAAGAGAC GTTCAAACCG GCAAGTGAAA CGGAAGAAGT ACACTGAGGA TTTGGACATT 
AAGATCACAG ATGATGAAGA TGAATTGGAT GCAGATGTTG ATGTGACCAC AACTCCTATG 
CCCGCAGTAG GGCATGTCCA GCCTTTGGGA GCAGAGCTCC CCCCGGAGCT GGATGGGGAT 
GGTCTTCCAA GCATGCAGTT TTTTGTGGAG AACCCAAGTG AGGAGGATGC TGCTATTGTG 
GATAAAATAT TGTCTATGCG GGTGACCAAA AAGGAGGTGT GTCCAGGGCA GTATACTAAT 
GTAGAGGAGT TTTTTGTCAA ATATAAAAAC TATTCGTACA TGCACTGTGA ATGGGCCAGT 
CTTGAGCAGC TGGAAAGAGA CAAGAGGATC CATCAGAAGC TTAAGAGATT CAAGACGAAA 
CAGGCACAAA TGAGGAACCT GTTCCAAGAG GATGAGGAAC CTTTCAATCC AGATTATGTG 
GAAGTTGATC GTATTTTGGA CGAGTCACAT AGTGTTGATA AAGACAACGG GGAGCCAGTT 
GTGTACTACC TGGTGAAGTG GTGCTCTCTA CCCTATGAAG ATGCTACTTG GGAGCTGAAG 
GAGGATGTTG ATGAAGGCAA AGTGGAAGAA TTCAGGAAGA TTGAAAGCCG CCAACCACGA 
CTCAAGAGAA CTCCTCGGCC TGCTGCAAGT GCCTGGAAAA AGCTAGATGA GTCCACCGAG 
TATAAGAATG GGAACCAGCT CAGAGAATAC CAGCTTGAGG GAGTCAACTG GCTGCTCTTT 
AACTGGTACA ACAGGCAAAA CTGCATCCTA GCGGATGAAA TGGGTCTGGG GAAGACAATT 
CAGTCCATTG CCCTGCTGTC AGAGATGTTT TCTGCGGGGG TCCAGAGCCC GTTCATGATC 
ATTGCCCCTC TGTCCACGAT CACAAACTGG GAAAGAGAGT TTTCGAACTG GACTGATATG 
AATGCTATTG TTTATCACGG CAGTCTGGCT AGTAGACAGA TGATCCAGCA GTATGAAATG 
TACTGCAAAG ATGACAAGGG ACACTTGATA CCAGGGGCCT ATAAGTTTGA CGCTCTGATC 
ACAACCTTTG AGATGATTCT ATCAGACTGC CCAGAGCTGA GGGAGATTTC CTGGCGCTGT 
GTAGTTATTG ATGAAGCTCA CCGTTTGAAG AACAGAAACT GCAAACTCCT CGACAGTCTC 
AAGATGCTTG AAATTGAGCA TAAAGTGTTG CTTACTGGCA CTCCACTACA AAACACAGTA 
GAGGAGCTCT TCAGTCTGCT GCACTTTCTG GAACCGGCCC AGTTTCCCTC AGAGATAGAG 
TTCTTGCGAG AGTTTGGAGA TCTTAAAACT GAGGAGCAGG TGCAGAAACT ACAGTCAATC 
CTGAAGCCCA TGATGCTGCG CAGACTGAAG GAAGATGTAG AAAAGAACCT TGCCCCCAAG 
CAAGAAACTA TTATTGAGGT GGAGTTGACC GATGTACAGA AAAAGTATTA CCGTGCTATT 
CTGGAGAGAA ACTTCAGCTT CCTTAGCATG GGAGCCACAC AAAACAGCAA TGTACCCAAT 
CTCCTCAACA CAATGATGGA ACTTCGAAAG TGCTGCAACC ACCCATACCT CATTACAGGT 
GCTGAGGAGA AGATCGTGTC TGAGCTGAGG GAGGTGTATG ATCCTCTGGC TCCGGACTTT 
CATCTCCAGG CTCTGGTGCG CTCTGCAGGG AAACTGGTCT TGTTGGATAA GCTGCTTCCT 
CGCCTCAAGG CTGGCGGGCA CAAGGTGCTC ATCTTCTCCC AGATGGTGAG GTGCCTTGAC 
ATCCTTGAGG ACTACCTCAT ACACAAGAGA TACCTGTATG AGCGCATTGA TGGACGGGTC 
AGAGGAAACC TGCGACAGGC GGCCATTGAT CGCTTCAGTA AACCTGATTC AGACCGGTTT 
GTCTTCCTGC TTTGTACTCG GGCTGGAGGT TTGGGCATTA ATCTGACCGC TGCTGACACC 
TGTGTCATTT TTGACTCTGA CTGGAACCCG CAGAATGATC TTCAAGCTCA GGCACGTTGC 
CATCGTATTG GTCAGTCTAA AGCGGTAAAG GTGTATCGTC TCATTACCAG AAACTCCTAC 
GAGAGGGAGA TGTTGGACAA AGCTAGTCTT AAACTGGGGC TTGACAGAGC CGTTCTGCAG 
AGCATGAGTG GAAACAAGGA AAGCAGTGTC AACGGGATCC AGCAGTTCTC TAAGAAGGAG 
ATTGAGGATT TGCTTCGTAA AGGTGCTTAT GCAGCCATAA TGGATGAGAA TGACGAGGGC 
AGTCGTTTCT GTGAAGAGGA CATCGACCAG ATCCTTCAGA GACGAGCAAC TACCATCACA 
ATAGAGAGCG AAGGCAAAGG ATCCACTTTT TCCAAGGCTA GCTTTGTGGC GTCTGAGAAC 
CGCACAGATA TCGCTCTAGA TGATCCCGAG TTCTGGCAGA AGTGGGCTAA GAAAGCAGAC 
ATAGACATGG ATTCTCTCAA CAGGAAGAAT ACTCTTGTCA TCGACACACC AAGAGTAAGA 
AAGCAAACAC GTCAGTTTTC CAGCCTGCGT GGAGAGGGTG GTGATCTCTC TGACTTGGAC 
AGCGATGATG ACTACCCACC CCATAATTCC CGTCAATCCA GAGCCTCCAG ACGCTCTGAC 
CGGCATTCTG GAGGAGGCTA TGGTCGCACA GACTGTTTCC GAGTGGAGAA ACATCTACTC 
GTTTATGGGT GGGGTCGTTG GCGAGACATC CTGTCGCATG CTCGCTGTAA GCGGCGTCTC 
AGTGAACGGG ACGTAGAAAC CATCTGCCGT GTCATCCTGG TTTTCTGTCT GATTCATTAT 
CGTGGAGATG AGAACATCAA GAGCTTCATT TGGGAGCTGA TTACCCCTCC AGAGAATGGA 
CGAGAACCAC AAGCTCTGCT CAATCACTCT GGTCTGTCCA TCCCTGTTCC TCGCGGCCGA 
AAGGGAAAGA GAGTGAAGGC TCAGAGCTCT TTTGACGTGC AGAAGGTTGA GTGGATCCGC 
AAATACAATC CTGACAGTCT GCTGCTGGAT GACAGTTACA GAAAACACCT CAAACACCAG 
TGCAACAAGG TTTTGCTGAG AGTACGTATG CTTTATTATC TGAAGCAGGA GGTCATTGGA 
GAACATGCTG ATTCTGTATT GAGTGGAGCT GATGCAAGGG ATATTGATAT CTGGTTGCCT 
GAAATGGAGC AGCAGGATGT TCCATCTGGC TGGTGGGATG CAGAAGCTGA CCGATGTTTG 
CTTATCGGAG TCTACAAGCA TGGATATGAG ATGTACACCA CCATGCGTGC AGACCCCTGC 
TTGTGTTTTG TTGAGCGATG TGGTCGTCCA AATGAGCAGG ACATTAATGC AGAGCAGCAA 
GCAGCAGACC CAGAGCTTGG AGAGGGAGGG GACTACGACA AGTACTCTGA AGATCCAGAG 
TTCAAACCCG CAACGAGACA TGCCAAGGAG ATGTATGAGG AGGGTGATTC GGTGAATGCA 
GATGGTGAGA TTTGTGTGGA GGATCGATCT GCCCCTATGC AAGTTGAAGG ACCGTCTTCG 
GGATCATCTG ATTTGTGTTA CTGGCCCACC AGCTCATCGC TCACAGCCCG ACTGCGACGT 
CTTATCACAG CTTACCAGCG CAGTTACAGA CGAGAGCAGC TTAAGATCGA GGCAGCAGAG 
AAGGGGGACC GTAGGCGAAG ACGCTGCGAA CAAGCTACCA AACTCAAAGA GATAGCCAGA 
CAAGAGCGAC AGCAACGGTG GACTCGTAGA GAAGAGTGCG ACTTTTACAG GGTGGTATCG 
ACATTTGGGG TGGAAAGGAT AAAGAAAGAA ACAGATGCTC CAGAAGGGGA CGAGCATCAC 
ATGGACTGGA ATCGTTTCCG TTCTTTTGCT CGACTCGATA AGAAAACCGA TGAGAGCTTG 
ACACGTTACT TTAAGTGCTT CATGTCCATG TGTCGGAAAG TGTGTCACAT TCGGCCAGGA 
CGTGGAGACG AATCTCAAGA TATGTCTCAG TCTCTGGCCC CGATCACAGA AGAGCGAGCC 
TCACGAACTC TCTACAGGGT CACCCTGCTG TGTCGGTTGC GTGAGCGTGT TCTTCCTCAC 
CCCTCGCTCG AGGAACGCCT CAGTCTGGCA CCACAAACCT CTGACCTGCC CAGTTGGTGG 
AGCATCCCAA AGCATGACCA CGAGCTTCTC TTAGCTGCTG CCAGACATGG AGTGAGCCGC 
ACTGAACTCT CCATCTTCTC TGACCCACTG TACTCCTTCA GTCAGAGCCG CCTCGACTAC 
CTCCAGAACC AGCAGGCTCA AGCAGCTGCA CAAATCCACG CCTTCAGTCA GTCACAGGAT 
CCAGCCGGCA TCAAAGAAGA GGGCCTGGAG GATGAGTCTC GACTGCTGGG TGTGGAGGCT 
CTCTGTCCGT CAGACTCTCC GGCCATGCTG CTCTCTCATT CTGACAGCAA AGTTGGCATT 
CAGGCTGGCT GGGTCTGGAA GAAGAGCAAA AACAACGGGC CGAGTGAGAG GAAATTGGGA 
GGGGGAGGAG GAGGGGCTTC AGACTCTGAT TCTGATTCAG ATTCAGGCTC GTCTTCCTCC 
TCTCGGCATT CAGGCAGCTC AGATGATAGT GGAGACAGTG ATGTGGAGAG AGAACAAGCA 
GCAGCTCTTA AGATGTGTGA TGGTGATGAA GAAAACAGCA TCCTCTCACT CACGCCGTCA 
CAGGAAGGAG CTCCACCTGA GTCCCTCACT GACCCTCTAA GAGTGGACTG GCCCAAAGAC 
CGCATTCTGA TCAACAGGAT AGAAAACCTC TGTTCACTGG TTATTACGGG ACACTGGCCG 
TCAGGTCGCC GCTACATCTC TGACATCCAA CTTAACACTG TATCTGATGA GCATGAACTC 
GGAGATGACC TCGGTTACTC ACGGGTGGCA CGAAAAATCA ACAGTACCTT ATCTGCAGAG 
GCTCTCGAAG GCCAGGAGTC AGAGTTTACA GTAAAGCTCC TAAAGGAGGA AGGCCTAAAG 
CTGACCTTCT CCAAGCAGGC TCTGATGCCA AACGGTGAAG GGAGTGCACG CAAAAAGCGA 
AAGGACCATG AGCTGGAAGA TGCAGAAGGG GTTCTCCACG CTCCCCGCAG GAGGGACCTT 
CCCAACTGGC TCAAAGAAAA CCCAGATTAT GAGGTTGAGG GAGATATGCT AGAATTACTG 
GTGAACCGGA CCAAAAGGAA GAGGAGGAGG AAGAGGGTGG AGAAAGGAGC AGCACTTACT 
GGCAGCGAGA GAGTGAAGGT CATAGACATA CGGACAGGCA AGAAGTTTGC TGGAGTGTTC 
GGGCCTGCAC TACAGGACCT GAGAGAGCAC CTGGAGGAGA ATCCTGACCA CGCTGTAGCA 
CCGGAATGGT CTGAGACTGT TCGCCACTCG GGCTTCTTGC CTGAGATTTT ATTCCACAGA 
CTGTTGAGTC CCCACGCTTC AATCCCCAAA AAGAGCCGGC ATTACCTCCA CACCCCTTCC 
CTTCAGACCG ACGACCCTCT CCTGGGAGGC GGTGAAGGTG AGATGCTAGT TTCTGACGGC 
GCCTACATGA TGGACGATGA AGACCTGGAG GACGGAGGTC ACCTCACGTC ATCTCACCAC 
TTCCTCACTC CAGCCTATGA TGTGAAAATG GAACCGAGCG CGCTCGATAT GGACGGAGGC 
GACAGTTTGT CACAGGGCGG TTATGATAGC TCTGACAGAG AGGCCATTTT GGATGATGTC 
ATCATGGCAC CCAAACATTC GGACACTTCC TCGAGCTCAG AGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD8 ( XM_021481014.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336689.1 Danio rerio chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.

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CloneID	ODa07226
Clone ID Related Accession (Same CDS sequence)	XM_021481014.1 , XM_005171313.3
Accession Version	XM_021481014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CTATTATGGA CCTCTTTGAT GACACACCAC TGTTCAATTT GGACTCCCTG 
CCGGAGGATG CGTTCTCCCA GGGTTCCTCA GACCCAGTAG AGGAGGCGCT GAAGTTGGCA 
TTGGGCCAAG TAGACCCTCC TACAGACCCC ATACCTGACC CTGGTGTTCC TATTCTGAGT 
GACGTGGTCA CAGATCCAGC TCTAATCCCA ACTCCTGTCT CTGTTCCACT GCAAAACCTT 
CAAACTCAGC AGCTCAGCCA GATCCCCCAT GAGGTCTCTG TTGCCTCTGC TCCCATTTCC 
ATTCAGCCCT CCCTCTCGGT GGCCAGTAAC AGCAGTGGTG CTGCAACAGT CTTGCTGAGC 
TCTTCTCTTG GTGTACCTGT GTCAGGGGCT CAAGTCACCC CTCAGCAGCA GACTCAGCAA 
ATCACTGCTG TTACGCAACA GGCAGCGGGA CAGCACGCCC CAAAAATTGT CATTCTGAAA 
GGCCCGCAGG GTCAGACTCA AGTGCTGCAG GGTGTCACAG GAGCCACGGG CTCTCCAGGG 
AAAGTCACCC TCGCTAGAGT TCTGACAGGG ACCCCTCTTA GGCCCGGCAT GGCAGTTGTT 
TCTGGAGGGA CGGTATTAAA TGCGACATCT CCTGCGCAGG GGCAGGTCAA AGTTGGCACT 
GGAGTACAAA GGCTGGTTCA AACTGCAAAT GGCCCAATGA AACAGGTGTT ACTGACCTCA 
GTGCCCCAGA CGCAATCGCA GGTGCAGACA CAGCCTGTCC AGGTGCAAAT ACCTGTGCAG 
ACACAGCTGC AGTCACCATC GCAGCCGCAA CAGCTTCAGG CGCAGATCCA GGCTCAGACA 
CAGGTGGCAT TACAAACACA AGCCCAAACG CAGACTCCTA CTTCTCCGGC AGCAGCAGGC 
ATCCGACCTC AAAGTGTCAC ACTTTCTGCA GTACCACAGC AGGGGGAAGC AAAGAGGATC 
ACTCTGGTGC TACAGCAGCC CTCACAAGGT GGAGCTGCCC AAGCTGGGAC GGTGACAGTA 
GGGGGAACTG CAGGGTCGGG TCAGCAAGGC CAGCAGCCCA GGCTGGTGTT GGGCTCTCTG 
CCAGGGAAGT TAGTCCTGCA GGGAGACCAG CTGGCAGCAC TCGCCCAAGC CAAGGCAGGT 
CAAACGGGTG CACAGGCAAA AGTGCTCACT ATTCAGCTGC AGGTTCAACA GCAGCCCAAC 
CAACAGGGAG CCAAGTTTCA GTTGGTTTCT GGGGCAGCAA ATGCTGGTGG CAGTCCACAG 
GTGGTGCAGA TTTCTCAAGG CCAAGGAGGA CAAAGACTAG CAGTGCCCCT TAAACTACTG 
CTGCAGCCAC AGTCAAACAC TGTTTCCAGC GCTGGTGGAG CTGTCTCTGT GGTTAAAGTT 
ATCAATACCT CAGCTGCTGG CTCGACGTCC GGCACCACCA CTACAGCTGC ATCTTCCGGG 
GTACGCTTGG CAAAGATCCA GGAGCCCGTA CGGAGAGTGG AAACGCTTTG CAAGCAAGAG 
AAAGCAAATC GCATTGTTGC TGAGGCCATC GCACGGGCCA AAGCCAGGGG TGAAAGGAAC 
ATCCCACGAG TGCTCAACCA GGATGAACTA CCTGCCGGAC AAACCTCAGC AGACCTAGAG 
GGCGCAGGAG GGGCTACCGG AGCCAAGAAG AAAGGAGGAG GAGGAGTTGG AGGTGGTGGA 
GGTGGAAGCA AGAAGAAAAG CCCAAGTGCA GGAGGAGCGA AAATGGTGGT GGGAGGAGAT 
AAGAAATCCA AGGCAAAAAC TCCAGTTATA CCTGGTGGAG GCAGTAAAAG CAAATCTAAG 
ACTAAGCTCA ACACCATCAC TCTAGTGGGT AAGAAAAGGA AAAGAAACCC GTCTTCTGAT 
CACTCAGACG TAGATCTCAG CCCACCTGTT TCGCCCCGCA CACTGGAGGA AGAAATGTCA 
CAGAAGAGAC GTTCAAACCG GCAAGTGAAA CGGAAGAAGT ACACTGAGGA TTTGGACATT 
AAGATCACAG ATGATGAAGA TGAATTGGAT GCAGATGTTG ATGTGACCAC AACTCCTATG 
CCCGCAGTAG GGCATGTCCA GCCTTTGGGA GCAGAGCTCC CCCCGGAGCT GGATGGGGAT 
GGTCTTCCAA GCATGCAGTT TTTTGTGGAG AACCCAAGTG AGGAGGATGC TGCTATTGTG 
GATAAAATAT TGTCTATGCG GGTGACCAAA AAGGAGGTGT GTCCAGGGCA GTATACTAAT 
GTAGAGGAGT TTTTTGTCAA ATATAAAAAC TATTCGTACA TGCACTGTGA ATGGGCCAGT 
CTTGAGCAGC TGGAAAGAGA CAAGAGGATC CATCAGAAGC TTAAGAGATT CAAGACGAAA 
CAGGCACAAA TGAGGAACCT GTTCCAAGAG GATGAGGAAC CTTTCAATCC AGATTATGTG 
GAAGTTGATC GTATTTTGGA CGAGTCACAT AGTGTTGATA AAGACAACGG GGAGCCAGTT 
GTGTACTACC TGGTGAAGTG GTGCTCTCTA CCCTATGAAG ATGCTACTTG GGAGCTGAAG 
GAGGATGTTG ATGAAGGCAA AGTGGAAGAA TTCAGGAAGA TTGAAAGCCG CCAACCACGA 
CTCAAGAGAA CTCCTCGGCC TGCTGCAAGT GCCTGGAAAA AGCTAGATGA GTCCACCGAG 
TATAAGAATG GGAACCAGCT CAGAGAATAC CAGCTTGAGG GAGTCAACTG GCTGCTCTTT 
AACTGGTACA ACAGGCAAAA CTGCATCCTA GCGGATGAAA TGGGTCTGGG GAAGACAATT 
CAGTCCATTG CCCTGCTGTC AGAGATGTTT TCTGCGGGGG TCCAGAGCCC GTTCATGATC 
ATTGCCCCTC TGTCCACGAT CACAAACTGG GAAAGAGAGT TTTCGAACTG GACTGATATG 
AATGCTATTG TTTATCACGG CAGTCTGGCT AGTAGACAGA TGATCCAGCA GTATGAAATG 
TACTGCAAAG ATGACAAGGG ACACTTGATA CCAGGGGCCT ATAAGTTTGA CGCTCTGATC 
ACAACCTTTG AGATGATTCT ATCAGACTGC CCAGAGCTGA GGGAGATTTC CTGGCGCTGT 
GTAGTTATTG ATGAAGCTCA CCGTTTGAAG AACAGAAACT GCAAACTCCT CGACAGTCTC 
AAGATGCTTG AAATTGAGCA TAAAGTGTTG CTTACTGGCA CTCCACTACA AAACACAGTA 
GAGGAGCTCT TCAGTCTGCT GCACTTTCTG GAACCGGCCC AGTTTCCCTC AGAGATAGAG 
TTCTTGCGAG AGTTTGGAGA TCTTAAAACT GAGGAGCAGG TGCAGAAACT ACAGTCAATC 
CTGAAGCCCA TGATGCTGCG CAGACTGAAG GAAGATGTAG AAAAGAACCT TGCCCCCAAG 
CAAGAAACTA TTATTGAGGT GGAGTTGACC GATGTACAGA AAAAGTATTA CCGTGCTATT 
CTGGAGAGAA ACTTCAGCTT CCTTAGCATG GGAGCCACAC AAAACAGCAA TGTACCCAAT 
CTCCTCAACA CAATGATGGA ACTTCGAAAG TGCTGCAACC ACCCATACCT CATTACAGGT 
GCTGAGGAGA AGATCGTGTC TGAGCTGAGG GAGGTGTATG ATCCTCTGGC TCCGGACTTT 
CATCTCCAGG CTCTGGTGCG CTCTGCAGGG AAACTGGTCT TGTTGGATAA GCTGCTTCCT 
CGCCTCAAGG CTGGCGGGCA CAAGGTGCTC ATCTTCTCCC AGATGGTGAG GTGCCTTGAC 
ATCCTTGAGG ACTACCTCAT ACACAAGAGA TACCTGTATG AGCGCATTGA TGGACGGGTC 
AGAGGAAACC TGCGACAGGC GGCCATTGAT CGCTTCAGTA AACCTGATTC AGACCGGTTT 
GTCTTCCTGC TTTGTACTCG GGCTGGAGGT TTGGGCATTA ATCTGACCGC TGCTGACACC 
TGTGTCATTT TTGACTCTGA CTGGAACCCG CAGAATGATC TTCAAGCTCA GGCACGTTGC 
CATCGTATTG GTCAGTCTAA AGCGGTAAAG GTGTATCGTC TCATTACCAG AAACTCCTAC 
GAGAGGGAGA TGTTGGACAA AGCTAGTCTT AAACTGGGGC TTGACAGAGC CGTTCTGCAG 
AGCATGAGTG GAAACAAGGA AAGCAGTGTC AACGGGATCC AGCAGTTCTC TAAGAAGGAG 
ATTGAGGATT TGCTTCGTAA AGGTGCTTAT GCAGCCATAA TGGATGAGAA TGACGAGGGC 
AGTCGTTTCT GTGAAGAGGA CATCGACCAG ATCCTTCAGA GACGAGCAAC TACCATCACA 
ATAGAGAGCG AAGGCAAAGG ATCCACTTTT TCCAAGGCTA GCTTTGTGGC GTCTGAGAAC 
CGCACAGATA TCGCTCTAGA TGATCCCGAG TTCTGGCAGA AGTGGGCTAA GAAAGCAGAC 
ATAGACATGG ATTCTCTCAA CAGGAAGAAT ACTCTTGTCA TCGACACACC AAGAGTAAGA 
AAGCAAACAC GTCAGTTTTC CAGCCTGCGT GGAGAGGGTG GTGATCTCTC TGACTTGGAC 
AGCGATGATG ACTACCCACC CCATAATTCC CGTCAATCCA GAGCCTCCAG ACGCTCTGAC 
CGGCATTCTG GAGGAGGCTA TGGTCGCACA GACTGTTTCC GAGTGGAGAA ACATCTACTC 
GTTTATGGGT GGGGTCGTTG GCGAGACATC CTGTCGCATG CTCGCTGTAA GCGGCGTCTC 
AGTGAACGGG ACGTAGAAAC CATCTGCCGT GTCATCCTGG TTTTCTGTCT GATTCATTAT 
CGTGGAGATG AGAACATCAA GAGCTTCATT TGGGAGCTGA TTACCCCTCC AGAGAATGGA 
CGAGAACCAC AAGCTCTGCT CAATCACTCT GGTCTGTCCA TCCCTGTTCC TCGCGGCCGA 
AAGGGAAAGA GAGTGAAGGC TCAGAGCTCT TTTGACGTGC AGAAGGTTGA GTGGATCCGC 
AAATACAATC CTGACAGTCT GCTGCTGGAT GACAGTTACA GAAAACACCT CAAACACCAG 
TGCAACAAGG TTTTGCTGAG AGTACGTATG CTTTATTATC TGAAGCAGGA GGTCATTGGA 
GAACATGCTG ATTCTGTATT GAGTGGAGCT GATGCAAGGG ATATTGATAT CTGGTTGCCT 
GAAATGGAGC AGCAGGATGT TCCATCTGGC TGGTGGGATG CAGAAGCTGA CCGATGTTTG 
CTTATCGGAG TCTACAAGCA TGGATATGAG ATGTACACCA CCATGCGTGC AGACCCCTGC 
TTGTGTTTTG TTGAGCGATG TGGTCGTCCA AATGAGCAGG ACATTAATGC AGAGCAGCAA 
GCAGCAGACC CAGAGCTTGG AGAGGGAGGG GACTACGACA AGTACTCTGA AGATCCAGAG 
TTCAAACCCG CAACGAGACA TGCCAAGGAG ATGTATGAGG AGGGTGATTC GGTGAATGCA 
GATGGTGAGA TTTGTGTGGA GGATCGATCT GCCCCTATGC AAGTTGAAGG ACCGTCTTCG 
GGATCATCTG ATTTGTGTTA CTGGCCCACC AGCTCATCGC TCACAGCCCG ACTGCGACGT 
CTTATCACAG CTTACCAGCG CAGTTACAGA CGAGAGCAGC TTAAGATCGA GGCAGCAGAG 
AAGGGGGACC GTAGGCGAAG ACGCTGCGAA CAAGCTACCA AACTCAAAGA GATAGCCAGA 
CAAGAGCGAC AGCAACGGTG GACTCGTAGA GAAGAGTGCG ACTTTTACAG GGTGGTATCG 
ACATTTGGGG TGGAAAGGAT AAAGAAAGAA ACAGATGCTC CAGAAGGGGA CGAGCATCAC 
ATGGACTGGA ATCGTTTCCG TTCTTTTGCT CGACTCGATA AGAAAACCGA TGAGAGCTTG 
ACACGTTACT TTAAGTGCTT CATGTCCATG TGTCGGAAAG TGTGTCACAT TCGGCCAGGA 
CGTGGAGACG AATCTCAAGA TATGTCTCAG TCTCTGGCCC CGATCACAGA AGAGCGAGCC 
TCACGAACTC TCTACAGGGT CACCCTGCTG TGTCGGTTGC GTGAGCGTGT TCTTCCTCAC 
CCCTCGCTCG AGGAACGCCT CAGTCTGGCA CCACAAACCT CTGACCTGCC CAGTTGGTGG 
AGCATCCCAA AGCATGACCA CGAGCTTCTC TTAGCTGCTG CCAGACATGG AGTGAGCCGC 
ACTGAACTCT CCATCTTCTC TGACCCACTG TACTCCTTCA GTCAGAGCCG CCTCGACTAC 
CTCCAGAACC AGCAGGCTCA AGCAGCTGCA CAAATCCACG CCTTCAGTCA GTCACAGGAT 
CCAGCCGGCA TCAAAGAAGA GGGCCTGGAG GATGAGTCTC GACTGCTGGG TGTGGAGGCT 
CTCTGTCCGT CAGACTCTCC GGCCATGCTG CTCTCTCATT CTGACAGCAA AGTTGGCATT 
CAGGCTGGCT GGGTCTGGAA GAAGAGCAAA AACAACGGGC CGAGTGAGAG GAAATTGGGA 
GGGGGAGGAG GAGGGGCTTC AGACTCTGAT TCTGATTCAG ATTCAGGCTC GTCTTCCTCC 
TCTCGGCATT CAGGCAGCTC AGATGATAGT GGAGACAGTG ATGTGGAGAG AGAACAAGCA 
GCTCTTAAGA TGTGTGATGG TGATGAAGAA AACAGCATCC TCTCACTCAC GCCGTCACAG 
GAAGGAGCTC CACCTGAGTC CCTCACTGAC CCTCTAAGAG TGGACTGGCC CAAAGACCGC 
ATTCTGATCA ACAGGATAGA AAACCTCTGT TCACTGGTTA TTACGGGACA CTGGCCGTCA 
GGTCGCCGCT ACATCTCTGA CATCCAACTT AACACTGTAT CTGATGAGCA TGAACTCGGA 
GATGACCTCG GTTACTCACG GGTGGCACGA AAAATCAACA GTACCTTATC TGCAGAGGCT 
CTCGAAGGCC AGGAGTCAGA GTTTACAGTA AAGCTCCTAA AGGAGGAAGG CCTAAAGCTG 
ACCTTCTCCA AGCAGGCTCT GATGCCAAAC GGTGAAGGGA GTGCACGCAA AAAGCGAAAG 
GACCATGAGC TGGAAGATGC AGAAGGGGTT CTCCACGCTC CCCGCAGGAG GGACCTTCCC 
AACTGGCTCA AAGAAAACCC AGATTATGAG GTTGAGGGAG ATATGCTAGA ATTACTGGTG 
AACCGGACCA AAAGGAAGAG GAGGAGGAAG AGGGTGGAGA AAGGAGCAGC ACTTACTGGC 
AGCGAGAGAG TGAAGGTCAT AGACATACGG ACAGGCAAGA AGTTTGCTGG AGTGTTCGGG 
CCTGCACTAC AGGACCTGAG AGAGCACCTG GAGGAGAATC CTGACCACGC TGTAGCACCG 
GAATGGTCTG AGACTGTTCG CCACTCGGGC TTCTTGCCTG AGATTTTATT CCACAGACTG 
TTGAGTCCCC ACGCTTCAAT CCCCAAAAAG AGCCGGCATT ACCTCCACAC CCCTTCCCTT 
CAGACCGACG ACCCTCTCCT GGGAGGCGGT GAAGGTGAGA TGCTAGTTTC TGACGGCGCC 
TACATGATGG ACGATGAAGA CCTGGAGGAC GGAGGTCACC TCACGTCATC TCACCACTTC 
CTCACTCCAG CCTATGATGT GAAAATGGAA CCGAGCGCGC TCGATATGGA CGGAGGCGAC 
AGTTTGTCAC AGGGCGGTTA TGATAGCTCT GACAGAGAGG CCATTTTGGA TGATGTCATC 
ATGGCACCCA AACATTCGGA CACTTCCTCG AGCTCAGAGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336689.1
CDS	383..8047
Translation	MADPIMDLFDDTPLFNLDSLPEDAFSQGSSDPVEEALKLALGQVDPPTDPIPDPGVPILSDVVTDPALIPTPVSVPLQNLQTQQLSQIPHEVSVASAPISIQPSLSVASNSSGAATVLLSSSLGVPVSGAQVTPQQQTQQITAVTQQAAGQHAPKIVILKGPQGQTQVLQGVTGATGSPGKVTLARVLTGTPLRPGMAVVSGGTVLNATSPAQGQVKVGTGVQRLVQTANGPMKQVLLTSVPQTQSQVQTQPVQVQIPVQTQLQSPSQPQQLQAQIQAQTQVALQTQAQTQTPTSPAAAGIRPQSVTLSAVPQQGEAKRITLVLQQPSQGGAAQAGTVTVGGTAGSGQQGQQPRLVLGSLPGKLVLQGDQLAALAQAKAGQTGAQAKVLTIQLQVQQQPNQQGAKFQLVSGAANAGGSPQVVQISQGQGGQRLAVPLKLLLQPQSNTVSSAGGAVSVVKVINTSAAGSTSGTTTTAASSGVRLAKIQEPVRRVETLCKQEKANRIVAEAIARAKARGERNIPRVLNQDELPAGQTSADLEGAGGATGAKKKGGGGVGGGGGGSKKKSPSAGGAKMVVGGDKKSKAKTPVIPGGGSKSKSKTKLNTITLVGKKRKRNPSSDHSDVDLSPPVSPRTLEEEMSQKRRSNRQVKRKKYTEDLDIKITDDEDELDADVDVTTTPMPAVGHVQPLGAELPPELDGDGLPSMQFFVENPSEEDAAIVDKILSMRVTKKEVCPGQYTNVEEFFVKYKNYSYMHCEWASLEQLERDKRIHQKLKRFKTKQAQMRNLFQEDEEPFNPDYVEVDRILDESHSVDKDNGEPVVYYLVKWCSLPYEDATWELKEDVDEGKVEEFRKIESRQPRLKRTPRPAASAWKKLDESTEYKNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIALLSEMFSAGVQSPFMIIAPLSTITNWEREFSNWTDMNAIVYHGSLASRQMIQQYEMYCKDDKGHLIPGAYKFDALITTFEMILSDCPELREISWRCVVIDEAHRLKNRNCKLLDSLKMLEIEHKVLLTGTPLQNTVEELFSLLHFLEPAQFPSEIEFLREFGDLKTEEQVQKLQSILKPMMLRRLKEDVEKNLAPKQETIIEVELTDVQKKYYRAILERNFSFLSMGATQNSNVPNLLNTMMELRKCCNHPYLITGAEEKIVSELREVYDPLAPDFHLQALVRSAGKLVLLDKLLPRLKAGGHKVLIFSQMVRCLDILEDYLIHKRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCVIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMLDKASLKLGLDRAVLQSMSGNKESSVNGIQQFSKKEIEDLLRKGAYAAIMDENDEGSRFCEEDIDQILQRRATTITIESEGKGSTFSKASFVASENRTDIALDDPEFWQKWAKKADIDMDSLNRKNTLVIDTPRVRKQTRQFSSLRGEGGDLSDLDSDDDYPPHNSRQSRASRRSDRHSGGGYGRTDCFRVEKHLLVYGWGRWRDILSHARCKRRLSERDVETICRVILVFCLIHYRGDENIKSFIWELITPPENGREPQALLNHSGLSIPVPRGRKGKRVKAQSSFDVQKVEWIRKYNPDSLLLDDSYRKHLKHQCNKVLLRVRMLYYLKQEVIGEHADSVLSGADARDIDIWLPEMEQQDVPSGWWDAEADRCLLIGVYKHGYEMYTTMRADPCLCFVERCGRPNEQDINAEQQAADPELGEGGDYDKYSEDPEFKPATRHAKEMYEEGDSVNADGEICVEDRSAPMQVEGPSSGSSDLCYWPTSSSLTARLRRLITAYQRSYRREQLKIEAAEKGDRRRRRCEQATKLKEIARQERQQRWTRREECDFYRVVSTFGVERIKKETDAPEGDEHHMDWNRFRSFARLDKKTDESLTRYFKCFMSMCRKVCHIRPGRGDESQDMSQSLAPITEERASRTLYRVTLLCRLRERVLPHPSLEERLSLAPQTSDLPSWWSIPKHDHELLLAAARHGVSRTELSIFSDPLYSFSQSRLDYLQNQQAQAAAQIHAFSQSQDPAGIKEEGLEDESRLLGVEALCPSDSPAMLLSHSDSKVGIQAGWVWKKSKNNGPSERKLGGGGGGASDSDSDSDSGSSSSSRHSGSSDDSGDSDVEREQAALKMCDGDEENSILSLTPSQEGAPPESLTDPLRVDWPKDRILINRIENLCSLVITGHWPSGRRYISDIQLNTVSDEHELGDDLGYSRVARKINSTLSAEALEGQESEFTVKLLKEEGLKLTFSKQALMPNGEGSARKKRKDHELEDAEGVLHAPRRRDLPNWLKENPDYEVEGDMLELLVNRTKRKRRRKRVEKGAALTGSERVKVIDIRTGKKFAGVFGPALQDLREHLEENPDHAVAPEWSETVRHSGFLPEILFHRLLSPHASIPKKSRHYLHTPSLQTDDPLLGGGEGEMLVSDGAYMMDDEDLEDGGHLTSSHHFLTPAYDVKMEPSALDMDGGDSLSQGGYDSSDREAILDDVIMAPKHSDTSSSSED

Target ORF information:

RefSeq Version	XM_021481014.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481014.1

ORF Insert Sequence:

ATGGCAGACC CTATTATGGA CCTCTTTGAT GACACACCAC TGTTCAATTT GGACTCCCTG 
CCGGAGGATG CGTTCTCCCA GGGTTCCTCA GACCCAGTAG AGGAGGCGCT GAAGTTGGCA 
TTGGGCCAAG TAGACCCTCC TACAGACCCC ATACCTGACC CTGGTGTTCC TATTCTGAGT 
GACGTGGTCA CAGATCCAGC TCTAATCCCA ACTCCTGTCT CTGTTCCACT GCAAAACCTT 
CAAACTCAGC AGCTCAGCCA GATCCCCCAT GAGGTCTCTG TTGCCTCTGC TCCCATTTCC 
ATTCAGCCCT CCCTCTCGGT GGCCAGTAAC AGCAGTGGTG CTGCAACAGT CTTGCTGAGC 
TCTTCTCTTG GTGTACCTGT GTCAGGGGCT CAAGTCACCC CTCAGCAGCA GACTCAGCAA 
ATCACTGCTG TTACGCAACA GGCAGCGGGA CAGCACGCCC CAAAAATTGT CATTCTGAAA 
GGCCCGCAGG GTCAGACTCA AGTGCTGCAG GGTGTCACAG GAGCCACGGG CTCTCCAGGG 
AAAGTCACCC TCGCTAGAGT TCTGACAGGG ACCCCTCTTA GGCCCGGCAT GGCAGTTGTT 
TCTGGAGGGA CGGTATTAAA TGCGACATCT CCTGCGCAGG GGCAGGTCAA AGTTGGCACT 
GGAGTACAAA GGCTGGTTCA AACTGCAAAT GGCCCAATGA AACAGGTGTT ACTGACCTCA 
GTGCCCCAGA CGCAATCGCA GGTGCAGACA CAGCCTGTCC AGGTGCAAAT ACCTGTGCAG 
ACACAGCTGC AGTCACCATC GCAGCCGCAA CAGCTTCAGG CGCAGATCCA GGCTCAGACA 
CAGGTGGCAT TACAAACACA AGCCCAAACG CAGACTCCTA CTTCTCCGGC AGCAGCAGGC 
ATCCGACCTC AAAGTGTCAC ACTTTCTGCA GTACCACAGC AGGGGGAAGC AAAGAGGATC 
ACTCTGGTGC TACAGCAGCC CTCACAAGGT GGAGCTGCCC AAGCTGGGAC GGTGACAGTA 
GGGGGAACTG CAGGGTCGGG TCAGCAAGGC CAGCAGCCCA GGCTGGTGTT GGGCTCTCTG 
CCAGGGAAGT TAGTCCTGCA GGGAGACCAG CTGGCAGCAC TCGCCCAAGC CAAGGCAGGT 
CAAACGGGTG CACAGGCAAA AGTGCTCACT ATTCAGCTGC AGGTTCAACA GCAGCCCAAC 
CAACAGGGAG CCAAGTTTCA GTTGGTTTCT GGGGCAGCAA ATGCTGGTGG CAGTCCACAG 
GTGGTGCAGA TTTCTCAAGG CCAAGGAGGA CAAAGACTAG CAGTGCCCCT TAAACTACTG 
CTGCAGCCAC AGTCAAACAC TGTTTCCAGC GCTGGTGGAG CTGTCTCTGT GGTTAAAGTT 
ATCAATACCT CAGCTGCTGG CTCGACGTCC GGCACCACCA CTACAGCTGC ATCTTCCGGG 
GTACGCTTGG CAAAGATCCA GGAGCCCGTA CGGAGAGTGG AAACGCTTTG CAAGCAAGAG 
AAAGCAAATC GCATTGTTGC TGAGGCCATC GCACGGGCCA AAGCCAGGGG TGAAAGGAAC 
ATCCCACGAG TGCTCAACCA GGATGAACTA CCTGCCGGAC AAACCTCAGC AGACCTAGAG 
GGCGCAGGAG GGGCTACCGG AGCCAAGAAG AAAGGAGGAG GAGGAGTTGG AGGTGGTGGA 
GGTGGAAGCA AGAAGAAAAG CCCAAGTGCA GGAGGAGCGA AAATGGTGGT GGGAGGAGAT 
AAGAAATCCA AGGCAAAAAC TCCAGTTATA CCTGGTGGAG GCAGTAAAAG CAAATCTAAG 
ACTAAGCTCA ACACCATCAC TCTAGTGGGT AAGAAAAGGA AAAGAAACCC GTCTTCTGAT 
CACTCAGACG TAGATCTCAG CCCACCTGTT TCGCCCCGCA CACTGGAGGA AGAAATGTCA 
CAGAAGAGAC GTTCAAACCG GCAAGTGAAA CGGAAGAAGT ACACTGAGGA TTTGGACATT 
AAGATCACAG ATGATGAAGA TGAATTGGAT GCAGATGTTG ATGTGACCAC AACTCCTATG 
CCCGCAGTAG GGCATGTCCA GCCTTTGGGA GCAGAGCTCC CCCCGGAGCT GGATGGGGAT 
GGTCTTCCAA GCATGCAGTT TTTTGTGGAG AACCCAAGTG AGGAGGATGC TGCTATTGTG 
GATAAAATAT TGTCTATGCG GGTGACCAAA AAGGAGGTGT GTCCAGGGCA GTATACTAAT 
GTAGAGGAGT TTTTTGTCAA ATATAAAAAC TATTCGTACA TGCACTGTGA ATGGGCCAGT 
CTTGAGCAGC TGGAAAGAGA CAAGAGGATC CATCAGAAGC TTAAGAGATT CAAGACGAAA 
CAGGCACAAA TGAGGAACCT GTTCCAAGAG GATGAGGAAC CTTTCAATCC AGATTATGTG 
GAAGTTGATC GTATTTTGGA CGAGTCACAT AGTGTTGATA AAGACAACGG GGAGCCAGTT 
GTGTACTACC TGGTGAAGTG GTGCTCTCTA CCCTATGAAG ATGCTACTTG GGAGCTGAAG 
GAGGATGTTG ATGAAGGCAA AGTGGAAGAA TTCAGGAAGA TTGAAAGCCG CCAACCACGA 
CTCAAGAGAA CTCCTCGGCC TGCTGCAAGT GCCTGGAAAA AGCTAGATGA GTCCACCGAG 
TATAAGAATG GGAACCAGCT CAGAGAATAC CAGCTTGAGG GAGTCAACTG GCTGCTCTTT 
AACTGGTACA ACAGGCAAAA CTGCATCCTA GCGGATGAAA TGGGTCTGGG GAAGACAATT 
CAGTCCATTG CCCTGCTGTC AGAGATGTTT TCTGCGGGGG TCCAGAGCCC GTTCATGATC 
ATTGCCCCTC TGTCCACGAT CACAAACTGG GAAAGAGAGT TTTCGAACTG GACTGATATG 
AATGCTATTG TTTATCACGG CAGTCTGGCT AGTAGACAGA TGATCCAGCA GTATGAAATG 
TACTGCAAAG ATGACAAGGG ACACTTGATA CCAGGGGCCT ATAAGTTTGA CGCTCTGATC 
ACAACCTTTG AGATGATTCT ATCAGACTGC CCAGAGCTGA GGGAGATTTC CTGGCGCTGT 
GTAGTTATTG ATGAAGCTCA CCGTTTGAAG AACAGAAACT GCAAACTCCT CGACAGTCTC 
AAGATGCTTG AAATTGAGCA TAAAGTGTTG CTTACTGGCA CTCCACTACA AAACACAGTA 
GAGGAGCTCT TCAGTCTGCT GCACTTTCTG GAACCGGCCC AGTTTCCCTC AGAGATAGAG 
TTCTTGCGAG AGTTTGGAGA TCTTAAAACT GAGGAGCAGG TGCAGAAACT ACAGTCAATC 
CTGAAGCCCA TGATGCTGCG CAGACTGAAG GAAGATGTAG AAAAGAACCT TGCCCCCAAG 
CAAGAAACTA TTATTGAGGT GGAGTTGACC GATGTACAGA AAAAGTATTA CCGTGCTATT 
CTGGAGAGAA ACTTCAGCTT CCTTAGCATG GGAGCCACAC AAAACAGCAA TGTACCCAAT 
CTCCTCAACA CAATGATGGA ACTTCGAAAG TGCTGCAACC ACCCATACCT CATTACAGGT 
GCTGAGGAGA AGATCGTGTC TGAGCTGAGG GAGGTGTATG ATCCTCTGGC TCCGGACTTT 
CATCTCCAGG CTCTGGTGCG CTCTGCAGGG AAACTGGTCT TGTTGGATAA GCTGCTTCCT 
CGCCTCAAGG CTGGCGGGCA CAAGGTGCTC ATCTTCTCCC AGATGGTGAG GTGCCTTGAC 
ATCCTTGAGG ACTACCTCAT ACACAAGAGA TACCTGTATG AGCGCATTGA TGGACGGGTC 
AGAGGAAACC TGCGACAGGC GGCCATTGAT CGCTTCAGTA AACCTGATTC AGACCGGTTT 
GTCTTCCTGC TTTGTACTCG GGCTGGAGGT TTGGGCATTA ATCTGACCGC TGCTGACACC 
TGTGTCATTT TTGACTCTGA CTGGAACCCG CAGAATGATC TTCAAGCTCA GGCACGTTGC 
CATCGTATTG GTCAGTCTAA AGCGGTAAAG GTGTATCGTC TCATTACCAG AAACTCCTAC 
GAGAGGGAGA TGTTGGACAA AGCTAGTCTT AAACTGGGGC TTGACAGAGC CGTTCTGCAG 
AGCATGAGTG GAAACAAGGA AAGCAGTGTC AACGGGATCC AGCAGTTCTC TAAGAAGGAG 
ATTGAGGATT TGCTTCGTAA AGGTGCTTAT GCAGCCATAA TGGATGAGAA TGACGAGGGC 
AGTCGTTTCT GTGAAGAGGA CATCGACCAG ATCCTTCAGA GACGAGCAAC TACCATCACA 
ATAGAGAGCG AAGGCAAAGG ATCCACTTTT TCCAAGGCTA GCTTTGTGGC GTCTGAGAAC 
CGCACAGATA TCGCTCTAGA TGATCCCGAG TTCTGGCAGA AGTGGGCTAA GAAAGCAGAC 
ATAGACATGG ATTCTCTCAA CAGGAAGAAT ACTCTTGTCA TCGACACACC AAGAGTAAGA 
AAGCAAACAC GTCAGTTTTC CAGCCTGCGT GGAGAGGGTG GTGATCTCTC TGACTTGGAC 
AGCGATGATG ACTACCCACC CCATAATTCC CGTCAATCCA GAGCCTCCAG ACGCTCTGAC 
CGGCATTCTG GAGGAGGCTA TGGTCGCACA GACTGTTTCC GAGTGGAGAA ACATCTACTC 
GTTTATGGGT GGGGTCGTTG GCGAGACATC CTGTCGCATG CTCGCTGTAA GCGGCGTCTC 
AGTGAACGGG ACGTAGAAAC CATCTGCCGT GTCATCCTGG TTTTCTGTCT GATTCATTAT 
CGTGGAGATG AGAACATCAA GAGCTTCATT TGGGAGCTGA TTACCCCTCC AGAGAATGGA 
CGAGAACCAC AAGCTCTGCT CAATCACTCT GGTCTGTCCA TCCCTGTTCC TCGCGGCCGA 
AAGGGAAAGA GAGTGAAGGC TCAGAGCTCT TTTGACGTGC AGAAGGTTGA GTGGATCCGC 
AAATACAATC CTGACAGTCT GCTGCTGGAT GACAGTTACA GAAAACACCT CAAACACCAG 
TGCAACAAGG TTTTGCTGAG AGTACGTATG CTTTATTATC TGAAGCAGGA GGTCATTGGA 
GAACATGCTG ATTCTGTATT GAGTGGAGCT GATGCAAGGG ATATTGATAT CTGGTTGCCT 
GAAATGGAGC AGCAGGATGT TCCATCTGGC TGGTGGGATG CAGAAGCTGA CCGATGTTTG 
CTTATCGGAG TCTACAAGCA TGGATATGAG ATGTACACCA CCATGCGTGC AGACCCCTGC 
TTGTGTTTTG TTGAGCGATG TGGTCGTCCA AATGAGCAGG ACATTAATGC AGAGCAGCAA 
GCAGCAGACC CAGAGCTTGG AGAGGGAGGG GACTACGACA AGTACTCTGA AGATCCAGAG 
TTCAAACCCG CAACGAGACA TGCCAAGGAG ATGTATGAGG AGGGTGATTC GGTGAATGCA 
GATGGTGAGA TTTGTGTGGA GGATCGATCT GCCCCTATGC AAGTTGAAGG ACCGTCTTCG 
GGATCATCTG ATTTGTGTTA CTGGCCCACC AGCTCATCGC TCACAGCCCG ACTGCGACGT 
CTTATCACAG CTTACCAGCG CAGTTACAGA CGAGAGCAGC TTAAGATCGA GGCAGCAGAG 
AAGGGGGACC GTAGGCGAAG ACGCTGCGAA CAAGCTACCA AACTCAAAGA GATAGCCAGA 
CAAGAGCGAC AGCAACGGTG GACTCGTAGA GAAGAGTGCG ACTTTTACAG GGTGGTATCG 
ACATTTGGGG TGGAAAGGAT AAAGAAAGAA ACAGATGCTC CAGAAGGGGA CGAGCATCAC 
ATGGACTGGA ATCGTTTCCG TTCTTTTGCT CGACTCGATA AGAAAACCGA TGAGAGCTTG 
ACACGTTACT TTAAGTGCTT CATGTCCATG TGTCGGAAAG TGTGTCACAT TCGGCCAGGA 
CGTGGAGACG AATCTCAAGA TATGTCTCAG TCTCTGGCCC CGATCACAGA AGAGCGAGCC 
TCACGAACTC TCTACAGGGT CACCCTGCTG TGTCGGTTGC GTGAGCGTGT TCTTCCTCAC 
CCCTCGCTCG AGGAACGCCT CAGTCTGGCA CCACAAACCT CTGACCTGCC CAGTTGGTGG 
AGCATCCCAA AGCATGACCA CGAGCTTCTC TTAGCTGCTG CCAGACATGG AGTGAGCCGC 
ACTGAACTCT CCATCTTCTC TGACCCACTG TACTCCTTCA GTCAGAGCCG CCTCGACTAC 
CTCCAGAACC AGCAGGCTCA AGCAGCTGCA CAAATCCACG CCTTCAGTCA GTCACAGGAT 
CCAGCCGGCA TCAAAGAAGA GGGCCTGGAG GATGAGTCTC GACTGCTGGG TGTGGAGGCT 
CTCTGTCCGT CAGACTCTCC GGCCATGCTG CTCTCTCATT CTGACAGCAA AGTTGGCATT 
CAGGCTGGCT GGGTCTGGAA GAAGAGCAAA AACAACGGGC CGAGTGAGAG GAAATTGGGA 
GGGGGAGGAG GAGGGGCTTC AGACTCTGAT TCTGATTCAG ATTCAGGCTC GTCTTCCTCC 
TCTCGGCATT CAGGCAGCTC AGATGATAGT GGAGACAGTG ATGTGGAGAG AGAACAAGCA 
GCTCTTAAGA TGTGTGATGG TGATGAAGAA AACAGCATCC TCTCACTCAC GCCGTCACAG 
GAAGGAGCTC CACCTGAGTC CCTCACTGAC CCTCTAAGAG TGGACTGGCC CAAAGACCGC 
ATTCTGATCA ACAGGATAGA AAACCTCTGT TCACTGGTTA TTACGGGACA CTGGCCGTCA 
GGTCGCCGCT ACATCTCTGA CATCCAACTT AACACTGTAT CTGATGAGCA TGAACTCGGA 
GATGACCTCG GTTACTCACG GGTGGCACGA AAAATCAACA GTACCTTATC TGCAGAGGCT 
CTCGAAGGCC AGGAGTCAGA GTTTACAGTA AAGCTCCTAA AGGAGGAAGG CCTAAAGCTG 
ACCTTCTCCA AGCAGGCTCT GATGCCAAAC GGTGAAGGGA GTGCACGCAA AAAGCGAAAG 
GACCATGAGC TGGAAGATGC AGAAGGGGTT CTCCACGCTC CCCGCAGGAG GGACCTTCCC 
AACTGGCTCA AAGAAAACCC AGATTATGAG GTTGAGGGAG ATATGCTAGA ATTACTGGTG 
AACCGGACCA AAAGGAAGAG GAGGAGGAAG AGGGTGGAGA AAGGAGCAGC ACTTACTGGC 
AGCGAGAGAG TGAAGGTCAT AGACATACGG ACAGGCAAGA AGTTTGCTGG AGTGTTCGGG 
CCTGCACTAC AGGACCTGAG AGAGCACCTG GAGGAGAATC CTGACCACGC TGTAGCACCG 
GAATGGTCTG AGACTGTTCG CCACTCGGGC TTCTTGCCTG AGATTTTATT CCACAGACTG 
TTGAGTCCCC ACGCTTCAAT CCCCAAAAAG AGCCGGCATT ACCTCCACAC CCCTTCCCTT 
CAGACCGACG ACCCTCTCCT GGGAGGCGGT GAAGGTGAGA TGCTAGTTTC TGACGGCGCC 
TACATGATGG ACGATGAAGA CCTGGAGGAC GGAGGTCACC TCACGTCATC TCACCACTTC 
CTCACTCCAG CCTATGATGT GAAAATGGAA CCGAGCGCGC TCGATATGGA CGGAGGCGAC 
AGTTTGTCAC AGGGCGGTTA TGATAGCTCT GACAGAGAGG CCATTTTGGA TGATGTCATC 
ATGGCACCCA AACATTCGGA CACTTCCTCG AGCTCAGAGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD8 ( XM_005171313.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171370.1 Danio rerio chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.

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CloneID	ODa07226
Clone ID Related Accession (Same CDS sequence)	XM_021481014.1 , XM_005171313.3
Accession Version	XM_005171313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC CTATTATGGA CCTCTTTGAT GACACACCAC TGTTCAATTT GGACTCCCTG 
CCGGAGGATG CGTTCTCCCA GGGTTCCTCA GACCCAGTAG AGGAGGCGCT GAAGTTGGCA 
TTGGGCCAAG TAGACCCTCC TACAGACCCC ATACCTGACC CTGGTGTTCC TATTCTGAGT 
GACGTGGTCA CAGATCCAGC TCTAATCCCA ACTCCTGTCT CTGTTCCACT GCAAAACCTT 
CAAACTCAGC AGCTCAGCCA GATCCCCCAT GAGGTCTCTG TTGCCTCTGC TCCCATTTCC 
ATTCAGCCCT CCCTCTCGGT GGCCAGTAAC AGCAGTGGTG CTGCAACAGT CTTGCTGAGC 
TCTTCTCTTG GTGTACCTGT GTCAGGGGCT CAAGTCACCC CTCAGCAGCA GACTCAGCAA 
ATCACTGCTG TTACGCAACA GGCAGCGGGA CAGCACGCCC CAAAAATTGT CATTCTGAAA 
GGCCCGCAGG GTCAGACTCA AGTGCTGCAG GGTGTCACAG GAGCCACGGG CTCTCCAGGG 
AAAGTCACCC TCGCTAGAGT TCTGACAGGG ACCCCTCTTA GGCCCGGCAT GGCAGTTGTT 
TCTGGAGGGA CGGTATTAAA TGCGACATCT CCTGCGCAGG GGCAGGTCAA AGTTGGCACT 
GGAGTACAAA GGCTGGTTCA AACTGCAAAT GGCCCAATGA AACAGGTGTT ACTGACCTCA 
GTGCCCCAGA CGCAATCGCA GGTGCAGACA CAGCCTGTCC AGGTGCAAAT ACCTGTGCAG 
ACACAGCTGC AGTCACCATC GCAGCCGCAA CAGCTTCAGG CGCAGATCCA GGCTCAGACA 
CAGGTGGCAT TACAAACACA AGCCCAAACG CAGACTCCTA CTTCTCCGGC AGCAGCAGGC 
ATCCGACCTC AAAGTGTCAC ACTTTCTGCA GTACCACAGC AGGGGGAAGC AAAGAGGATC 
ACTCTGGTGC TACAGCAGCC CTCACAAGGT GGAGCTGCCC AAGCTGGGAC GGTGACAGTA 
GGGGGAACTG CAGGGTCGGG TCAGCAAGGC CAGCAGCCCA GGCTGGTGTT GGGCTCTCTG 
CCAGGGAAGT TAGTCCTGCA GGGAGACCAG CTGGCAGCAC TCGCCCAAGC CAAGGCAGGT 
CAAACGGGTG CACAGGCAAA AGTGCTCACT ATTCAGCTGC AGGTTCAACA GCAGCCCAAC 
CAACAGGGAG CCAAGTTTCA GTTGGTTTCT GGGGCAGCAA ATGCTGGTGG CAGTCCACAG 
GTGGTGCAGA TTTCTCAAGG CCAAGGAGGA CAAAGACTAG CAGTGCCCCT TAAACTACTG 
CTGCAGCCAC AGTCAAACAC TGTTTCCAGC GCTGGTGGAG CTGTCTCTGT GGTTAAAGTT 
ATCAATACCT CAGCTGCTGG CTCGACGTCC GGCACCACCA CTACAGCTGC ATCTTCCGGG 
GTACGCTTGG CAAAGATCCA GGAGCCCGTA CGGAGAGTGG AAACGCTTTG CAAGCAAGAG 
AAAGCAAATC GCATTGTTGC TGAGGCCATC GCACGGGCCA AAGCCAGGGG TGAAAGGAAC 
ATCCCACGAG TGCTCAACCA GGATGAACTA CCTGCCGGAC AAACCTCAGC AGACCTAGAG 
GGCGCAGGAG GGGCTACCGG AGCCAAGAAG AAAGGAGGAG GAGGAGTTGG AGGTGGTGGA 
GGTGGAAGCA AGAAGAAAAG CCCAAGTGCA GGAGGAGCGA AAATGGTGGT GGGAGGAGAT 
AAGAAATCCA AGGCAAAAAC TCCAGTTATA CCTGGTGGAG GCAGTAAAAG CAAATCTAAG 
ACTAAGCTCA ACACCATCAC TCTAGTGGGT AAGAAAAGGA AAAGAAACCC GTCTTCTGAT 
CACTCAGACG TAGATCTCAG CCCACCTGTT TCGCCCCGCA CACTGGAGGA AGAAATGTCA 
CAGAAGAGAC GTTCAAACCG GCAAGTGAAA CGGAAGAAGT ACACTGAGGA TTTGGACATT 
AAGATCACAG ATGATGAAGA TGAATTGGAT GCAGATGTTG ATGTGACCAC AACTCCTATG 
CCCGCAGTAG GGCATGTCCA GCCTTTGGGA GCAGAGCTCC CCCCGGAGCT GGATGGGGAT 
GGTCTTCCAA GCATGCAGTT TTTTGTGGAG AACCCAAGTG AGGAGGATGC TGCTATTGTG 
GATAAAATAT TGTCTATGCG GGTGACCAAA AAGGAGGTGT GTCCAGGGCA GTATACTAAT 
GTAGAGGAGT TTTTTGTCAA ATATAAAAAC TATTCGTACA TGCACTGTGA ATGGGCCAGT 
CTTGAGCAGC TGGAAAGAGA CAAGAGGATC CATCAGAAGC TTAAGAGATT CAAGACGAAA 
CAGGCACAAA TGAGGAACCT GTTCCAAGAG GATGAGGAAC CTTTCAATCC AGATTATGTG 
GAAGTTGATC GTATTTTGGA CGAGTCACAT AGTGTTGATA AAGACAACGG GGAGCCAGTT 
GTGTACTACC TGGTGAAGTG GTGCTCTCTA CCCTATGAAG ATGCTACTTG GGAGCTGAAG 
GAGGATGTTG ATGAAGGCAA AGTGGAAGAA TTCAGGAAGA TTGAAAGCCG CCAACCACGA 
CTCAAGAGAA CTCCTCGGCC TGCTGCAAGT GCCTGGAAAA AGCTAGATGA GTCCACCGAG 
TATAAGAATG GGAACCAGCT CAGAGAATAC CAGCTTGAGG GAGTCAACTG GCTGCTCTTT 
AACTGGTACA ACAGGCAAAA CTGCATCCTA GCGGATGAAA TGGGTCTGGG GAAGACAATT 
CAGTCCATTG CCCTGCTGTC AGAGATGTTT TCTGCGGGGG TCCAGAGCCC GTTCATGATC 
ATTGCCCCTC TGTCCACGAT CACAAACTGG GAAAGAGAGT TTTCGAACTG GACTGATATG 
AATGCTATTG TTTATCACGG CAGTCTGGCT AGTAGACAGA TGATCCAGCA GTATGAAATG 
TACTGCAAAG ATGACAAGGG ACACTTGATA CCAGGGGCCT ATAAGTTTGA CGCTCTGATC 
ACAACCTTTG AGATGATTCT ATCAGACTGC CCAGAGCTGA GGGAGATTTC CTGGCGCTGT 
GTAGTTATTG ATGAAGCTCA CCGTTTGAAG AACAGAAACT GCAAACTCCT CGACAGTCTC 
AAGATGCTTG AAATTGAGCA TAAAGTGTTG CTTACTGGCA CTCCACTACA AAACACAGTA 
GAGGAGCTCT TCAGTCTGCT GCACTTTCTG GAACCGGCCC AGTTTCCCTC AGAGATAGAG 
TTCTTGCGAG AGTTTGGAGA TCTTAAAACT GAGGAGCAGG TGCAGAAACT ACAGTCAATC 
CTGAAGCCCA TGATGCTGCG CAGACTGAAG GAAGATGTAG AAAAGAACCT TGCCCCCAAG 
CAAGAAACTA TTATTGAGGT GGAGTTGACC GATGTACAGA AAAAGTATTA CCGTGCTATT 
CTGGAGAGAA ACTTCAGCTT CCTTAGCATG GGAGCCACAC AAAACAGCAA TGTACCCAAT 
CTCCTCAACA CAATGATGGA ACTTCGAAAG TGCTGCAACC ACCCATACCT CATTACAGGT 
GCTGAGGAGA AGATCGTGTC TGAGCTGAGG GAGGTGTATG ATCCTCTGGC TCCGGACTTT 
CATCTCCAGG CTCTGGTGCG CTCTGCAGGG AAACTGGTCT TGTTGGATAA GCTGCTTCCT 
CGCCTCAAGG CTGGCGGGCA CAAGGTGCTC ATCTTCTCCC AGATGGTGAG GTGCCTTGAC 
ATCCTTGAGG ACTACCTCAT ACACAAGAGA TACCTGTATG AGCGCATTGA TGGACGGGTC 
AGAGGAAACC TGCGACAGGC GGCCATTGAT CGCTTCAGTA AACCTGATTC AGACCGGTTT 
GTCTTCCTGC TTTGTACTCG GGCTGGAGGT TTGGGCATTA ATCTGACCGC TGCTGACACC 
TGTGTCATTT TTGACTCTGA CTGGAACCCG CAGAATGATC TTCAAGCTCA GGCACGTTGC 
CATCGTATTG GTCAGTCTAA AGCGGTAAAG GTGTATCGTC TCATTACCAG AAACTCCTAC 
GAGAGGGAGA TGTTGGACAA AGCTAGTCTT AAACTGGGGC TTGACAGAGC CGTTCTGCAG 
AGCATGAGTG GAAACAAGGA AAGCAGTGTC AACGGGATCC AGCAGTTCTC TAAGAAGGAG 
ATTGAGGATT TGCTTCGTAA AGGTGCTTAT GCAGCCATAA TGGATGAGAA TGACGAGGGC 
AGTCGTTTCT GTGAAGAGGA CATCGACCAG ATCCTTCAGA GACGAGCAAC TACCATCACA 
ATAGAGAGCG AAGGCAAAGG ATCCACTTTT TCCAAGGCTA GCTTTGTGGC GTCTGAGAAC 
CGCACAGATA TCGCTCTAGA TGATCCCGAG TTCTGGCAGA AGTGGGCTAA GAAAGCAGAC 
ATAGACATGG ATTCTCTCAA CAGGAAGAAT ACTCTTGTCA TCGACACACC AAGAGTAAGA 
AAGCAAACAC GTCAGTTTTC CAGCCTGCGT GGAGAGGGTG GTGATCTCTC TGACTTGGAC 
AGCGATGATG ACTACCCACC CCATAATTCC CGTCAATCCA GAGCCTCCAG ACGCTCTGAC 
CGGCATTCTG GAGGAGGCTA TGGTCGCACA GACTGTTTCC GAGTGGAGAA ACATCTACTC 
GTTTATGGGT GGGGTCGTTG GCGAGACATC CTGTCGCATG CTCGCTGTAA GCGGCGTCTC 
AGTGAACGGG ACGTAGAAAC CATCTGCCGT GTCATCCTGG TTTTCTGTCT GATTCATTAT 
CGTGGAGATG AGAACATCAA GAGCTTCATT TGGGAGCTGA TTACCCCTCC AGAGAATGGA 
CGAGAACCAC AAGCTCTGCT CAATCACTCT GGTCTGTCCA TCCCTGTTCC TCGCGGCCGA 
AAGGGAAAGA GAGTGAAGGC TCAGAGCTCT TTTGACGTGC AGAAGGTTGA GTGGATCCGC 
AAATACAATC CTGACAGTCT GCTGCTGGAT GACAGTTACA GAAAACACCT CAAACACCAG 
TGCAACAAGG TTTTGCTGAG AGTACGTATG CTTTATTATC TGAAGCAGGA GGTCATTGGA 
GAACATGCTG ATTCTGTATT GAGTGGAGCT GATGCAAGGG ATATTGATAT CTGGTTGCCT 
GAAATGGAGC AGCAGGATGT TCCATCTGGC TGGTGGGATG CAGAAGCTGA CCGATGTTTG 
CTTATCGGAG TCTACAAGCA TGGATATGAG ATGTACACCA CCATGCGTGC AGACCCCTGC 
TTGTGTTTTG TTGAGCGATG TGGTCGTCCA AATGAGCAGG ACATTAATGC AGAGCAGCAA 
GCAGCAGACC CAGAGCTTGG AGAGGGAGGG GACTACGACA AGTACTCTGA AGATCCAGAG 
TTCAAACCCG CAACGAGACA TGCCAAGGAG ATGTATGAGG AGGGTGATTC GGTGAATGCA 
GATGGTGAGA TTTGTGTGGA GGATCGATCT GCCCCTATGC AAGTTGAAGG ACCGTCTTCG 
GGATCATCTG ATTTGTGTTA CTGGCCCACC AGCTCATCGC TCACAGCCCG ACTGCGACGT 
CTTATCACAG CTTACCAGCG CAGTTACAGA CGAGAGCAGC TTAAGATCGA GGCAGCAGAG 
AAGGGGGACC GTAGGCGAAG ACGCTGCGAA CAAGCTACCA AACTCAAAGA GATAGCCAGA 
CAAGAGCGAC AGCAACGGTG GACTCGTAGA GAAGAGTGCG ACTTTTACAG GGTGGTATCG 
ACATTTGGGG TGGAAAGGAT AAAGAAAGAA ACAGATGCTC CAGAAGGGGA CGAGCATCAC 
ATGGACTGGA ATCGTTTCCG TTCTTTTGCT CGACTCGATA AGAAAACCGA TGAGAGCTTG 
ACACGTTACT TTAAGTGCTT CATGTCCATG TGTCGGAAAG TGTGTCACAT TCGGCCAGGA 
CGTGGAGACG AATCTCAAGA TATGTCTCAG TCTCTGGCCC CGATCACAGA AGAGCGAGCC 
TCACGAACTC TCTACAGGGT CACCCTGCTG TGTCGGTTGC GTGAGCGTGT TCTTCCTCAC 
CCCTCGCTCG AGGAACGCCT CAGTCTGGCA CCACAAACCT CTGACCTGCC CAGTTGGTGG 
AGCATCCCAA AGCATGACCA CGAGCTTCTC TTAGCTGCTG CCAGACATGG AGTGAGCCGC 
ACTGAACTCT CCATCTTCTC TGACCCACTG TACTCCTTCA GTCAGAGCCG CCTCGACTAC 
CTCCAGAACC AGCAGGCTCA AGCAGCTGCA CAAATCCACG CCTTCAGTCA GTCACAGGAT 
CCAGCCGGCA TCAAAGAAGA GGGCCTGGAG GATGAGTCTC GACTGCTGGG TGTGGAGGCT 
CTCTGTCCGT CAGACTCTCC GGCCATGCTG CTCTCTCATT CTGACAGCAA AGTTGGCATT 
CAGGCTGGCT GGGTCTGGAA GAAGAGCAAA AACAACGGGC CGAGTGAGAG GAAATTGGGA 
GGGGGAGGAG GAGGGGCTTC AGACTCTGAT TCTGATTCAG ATTCAGGCTC GTCTTCCTCC 
TCTCGGCATT CAGGCAGCTC AGATGATAGT GGAGACAGTG ATGTGGAGAG AGAACAAGCA 
GCTCTTAAGA TGTGTGATGG TGATGAAGAA AACAGCATCC TCTCACTCAC GCCGTCACAG 
GAAGGAGCTC CACCTGAGTC CCTCACTGAC CCTCTAAGAG TGGACTGGCC CAAAGACCGC 
ATTCTGATCA ACAGGATAGA AAACCTCTGT TCACTGGTTA TTACGGGACA CTGGCCGTCA 
GGTCGCCGCT ACATCTCTGA CATCCAACTT AACACTGTAT CTGATGAGCA TGAACTCGGA 
GATGACCTCG GTTACTCACG GGTGGCACGA AAAATCAACA GTACCTTATC TGCAGAGGCT 
CTCGAAGGCC AGGAGTCAGA GTTTACAGTA AAGCTCCTAA AGGAGGAAGG CCTAAAGCTG 
ACCTTCTCCA AGCAGGCTCT GATGCCAAAC GGTGAAGGGA GTGCACGCAA AAAGCGAAAG 
GACCATGAGC TGGAAGATGC AGAAGGGGTT CTCCACGCTC CCCGCAGGAG GGACCTTCCC 
AACTGGCTCA AAGAAAACCC AGATTATGAG GTTGAGGGAG ATATGCTAGA ATTACTGGTG 
AACCGGACCA AAAGGAAGAG GAGGAGGAAG AGGGTGGAGA AAGGAGCAGC ACTTACTGGC 
AGCGAGAGAG TGAAGGTCAT AGACATACGG ACAGGCAAGA AGTTTGCTGG AGTGTTCGGG 
CCTGCACTAC AGGACCTGAG AGAGCACCTG GAGGAGAATC CTGACCACGC TGTAGCACCG 
GAATGGTCTG AGACTGTTCG CCACTCGGGC TTCTTGCCTG AGATTTTATT CCACAGACTG 
TTGAGTCCCC ACGCTTCAAT CCCCAAAAAG AGCCGGCATT ACCTCCACAC CCCTTCCCTT 
CAGACCGACG ACCCTCTCCT GGGAGGCGGT GAAGGTGAGA TGCTAGTTTC TGACGGCGCC 
TACATGATGG ACGATGAAGA CCTGGAGGAC GGAGGTCACC TCACGTCATC TCACCACTTC 
CTCACTCCAG CCTATGATGT GAAAATGGAA CCGAGCGCGC TCGATATGGA CGGAGGCGAC 
AGTTTGTCAC AGGGCGGTTA TGATAGCTCT GACAGAGAGG CCATTTTGGA TGATGTCATC 
ATGGCACCCA AACATTCGGA CACTTCCTCG AGCTCAGAGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171370.1
CDS	357..8021
Translation	MADPIMDLFDDTPLFNLDSLPEDAFSQGSSDPVEEALKLALGQVDPPTDPIPDPGVPILSDVVTDPALIPTPVSVPLQNLQTQQLSQIPHEVSVASAPISIQPSLSVASNSSGAATVLLSSSLGVPVSGAQVTPQQQTQQITAVTQQAAGQHAPKIVILKGPQGQTQVLQGVTGATGSPGKVTLARVLTGTPLRPGMAVVSGGTVLNATSPAQGQVKVGTGVQRLVQTANGPMKQVLLTSVPQTQSQVQTQPVQVQIPVQTQLQSPSQPQQLQAQIQAQTQVALQTQAQTQTPTSPAAAGIRPQSVTLSAVPQQGEAKRITLVLQQPSQGGAAQAGTVTVGGTAGSGQQGQQPRLVLGSLPGKLVLQGDQLAALAQAKAGQTGAQAKVLTIQLQVQQQPNQQGAKFQLVSGAANAGGSPQVVQISQGQGGQRLAVPLKLLLQPQSNTVSSAGGAVSVVKVINTSAAGSTSGTTTTAASSGVRLAKIQEPVRRVETLCKQEKANRIVAEAIARAKARGERNIPRVLNQDELPAGQTSADLEGAGGATGAKKKGGGGVGGGGGGSKKKSPSAGGAKMVVGGDKKSKAKTPVIPGGGSKSKSKTKLNTITLVGKKRKRNPSSDHSDVDLSPPVSPRTLEEEMSQKRRSNRQVKRKKYTEDLDIKITDDEDELDADVDVTTTPMPAVGHVQPLGAELPPELDGDGLPSMQFFVENPSEEDAAIVDKILSMRVTKKEVCPGQYTNVEEFFVKYKNYSYMHCEWASLEQLERDKRIHQKLKRFKTKQAQMRNLFQEDEEPFNPDYVEVDRILDESHSVDKDNGEPVVYYLVKWCSLPYEDATWELKEDVDEGKVEEFRKIESRQPRLKRTPRPAASAWKKLDESTEYKNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIALLSEMFSAGVQSPFMIIAPLSTITNWEREFSNWTDMNAIVYHGSLASRQMIQQYEMYCKDDKGHLIPGAYKFDALITTFEMILSDCPELREISWRCVVIDEAHRLKNRNCKLLDSLKMLEIEHKVLLTGTPLQNTVEELFSLLHFLEPAQFPSEIEFLREFGDLKTEEQVQKLQSILKPMMLRRLKEDVEKNLAPKQETIIEVELTDVQKKYYRAILERNFSFLSMGATQNSNVPNLLNTMMELRKCCNHPYLITGAEEKIVSELREVYDPLAPDFHLQALVRSAGKLVLLDKLLPRLKAGGHKVLIFSQMVRCLDILEDYLIHKRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCVIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMLDKASLKLGLDRAVLQSMSGNKESSVNGIQQFSKKEIEDLLRKGAYAAIMDENDEGSRFCEEDIDQILQRRATTITIESEGKGSTFSKASFVASENRTDIALDDPEFWQKWAKKADIDMDSLNRKNTLVIDTPRVRKQTRQFSSLRGEGGDLSDLDSDDDYPPHNSRQSRASRRSDRHSGGGYGRTDCFRVEKHLLVYGWGRWRDILSHARCKRRLSERDVETICRVILVFCLIHYRGDENIKSFIWELITPPENGREPQALLNHSGLSIPVPRGRKGKRVKAQSSFDVQKVEWIRKYNPDSLLLDDSYRKHLKHQCNKVLLRVRMLYYLKQEVIGEHADSVLSGADARDIDIWLPEMEQQDVPSGWWDAEADRCLLIGVYKHGYEMYTTMRADPCLCFVERCGRPNEQDINAEQQAADPELGEGGDYDKYSEDPEFKPATRHAKEMYEEGDSVNADGEICVEDRSAPMQVEGPSSGSSDLCYWPTSSSLTARLRRLITAYQRSYRREQLKIEAAEKGDRRRRRCEQATKLKEIARQERQQRWTRREECDFYRVVSTFGVERIKKETDAPEGDEHHMDWNRFRSFARLDKKTDESLTRYFKCFMSMCRKVCHIRPGRGDESQDMSQSLAPITEERASRTLYRVTLLCRLRERVLPHPSLEERLSLAPQTSDLPSWWSIPKHDHELLLAAARHGVSRTELSIFSDPLYSFSQSRLDYLQNQQAQAAAQIHAFSQSQDPAGIKEEGLEDESRLLGVEALCPSDSPAMLLSHSDSKVGIQAGWVWKKSKNNGPSERKLGGGGGGASDSDSDSDSGSSSSSRHSGSSDDSGDSDVEREQAALKMCDGDEENSILSLTPSQEGAPPESLTDPLRVDWPKDRILINRIENLCSLVITGHWPSGRRYISDIQLNTVSDEHELGDDLGYSRVARKINSTLSAEALEGQESEFTVKLLKEEGLKLTFSKQALMPNGEGSARKKRKDHELEDAEGVLHAPRRRDLPNWLKENPDYEVEGDMLELLVNRTKRKRRRKRVEKGAALTGSERVKVIDIRTGKKFAGVFGPALQDLREHLEENPDHAVAPEWSETVRHSGFLPEILFHRLLSPHASIPKKSRHYLHTPSLQTDDPLLGGGEGEMLVSDGAYMMDDEDLEDGGHLTSSHHFLTPAYDVKMEPSALDMDGGDSLSQGGYDSSDREAILDDVIMAPKHSDTSSSSED

Target ORF information:

RefSeq Version	XM_005171313.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171313.3

ORF Insert Sequence:

ATGGCAGACC CTATTATGGA CCTCTTTGAT GACACACCAC TGTTCAATTT GGACTCCCTG 
CCGGAGGATG CGTTCTCCCA GGGTTCCTCA GACCCAGTAG AGGAGGCGCT GAAGTTGGCA 
TTGGGCCAAG TAGACCCTCC TACAGACCCC ATACCTGACC CTGGTGTTCC TATTCTGAGT 
GACGTGGTCA CAGATCCAGC TCTAATCCCA ACTCCTGTCT CTGTTCCACT GCAAAACCTT 
CAAACTCAGC AGCTCAGCCA GATCCCCCAT GAGGTCTCTG TTGCCTCTGC TCCCATTTCC 
ATTCAGCCCT CCCTCTCGGT GGCCAGTAAC AGCAGTGGTG CTGCAACAGT CTTGCTGAGC 
TCTTCTCTTG GTGTACCTGT GTCAGGGGCT CAAGTCACCC CTCAGCAGCA GACTCAGCAA 
ATCACTGCTG TTACGCAACA GGCAGCGGGA CAGCACGCCC CAAAAATTGT CATTCTGAAA 
GGCCCGCAGG GTCAGACTCA AGTGCTGCAG GGTGTCACAG GAGCCACGGG CTCTCCAGGG 
AAAGTCACCC TCGCTAGAGT TCTGACAGGG ACCCCTCTTA GGCCCGGCAT GGCAGTTGTT 
TCTGGAGGGA CGGTATTAAA TGCGACATCT CCTGCGCAGG GGCAGGTCAA AGTTGGCACT 
GGAGTACAAA GGCTGGTTCA AACTGCAAAT GGCCCAATGA AACAGGTGTT ACTGACCTCA 
GTGCCCCAGA CGCAATCGCA GGTGCAGACA CAGCCTGTCC AGGTGCAAAT ACCTGTGCAG 
ACACAGCTGC AGTCACCATC GCAGCCGCAA CAGCTTCAGG CGCAGATCCA GGCTCAGACA 
CAGGTGGCAT TACAAACACA AGCCCAAACG CAGACTCCTA CTTCTCCGGC AGCAGCAGGC 
ATCCGACCTC AAAGTGTCAC ACTTTCTGCA GTACCACAGC AGGGGGAAGC AAAGAGGATC 
ACTCTGGTGC TACAGCAGCC CTCACAAGGT GGAGCTGCCC AAGCTGGGAC GGTGACAGTA 
GGGGGAACTG CAGGGTCGGG TCAGCAAGGC CAGCAGCCCA GGCTGGTGTT GGGCTCTCTG 
CCAGGGAAGT TAGTCCTGCA GGGAGACCAG CTGGCAGCAC TCGCCCAAGC CAAGGCAGGT 
CAAACGGGTG CACAGGCAAA AGTGCTCACT ATTCAGCTGC AGGTTCAACA GCAGCCCAAC 
CAACAGGGAG CCAAGTTTCA GTTGGTTTCT GGGGCAGCAA ATGCTGGTGG CAGTCCACAG 
GTGGTGCAGA TTTCTCAAGG CCAAGGAGGA CAAAGACTAG CAGTGCCCCT TAAACTACTG 
CTGCAGCCAC AGTCAAACAC TGTTTCCAGC GCTGGTGGAG CTGTCTCTGT GGTTAAAGTT 
ATCAATACCT CAGCTGCTGG CTCGACGTCC GGCACCACCA CTACAGCTGC ATCTTCCGGG 
GTACGCTTGG CAAAGATCCA GGAGCCCGTA CGGAGAGTGG AAACGCTTTG CAAGCAAGAG 
AAAGCAAATC GCATTGTTGC TGAGGCCATC GCACGGGCCA AAGCCAGGGG TGAAAGGAAC 
ATCCCACGAG TGCTCAACCA GGATGAACTA CCTGCCGGAC AAACCTCAGC AGACCTAGAG 
GGCGCAGGAG GGGCTACCGG AGCCAAGAAG AAAGGAGGAG GAGGAGTTGG AGGTGGTGGA 
GGTGGAAGCA AGAAGAAAAG CCCAAGTGCA GGAGGAGCGA AAATGGTGGT GGGAGGAGAT 
AAGAAATCCA AGGCAAAAAC TCCAGTTATA CCTGGTGGAG GCAGTAAAAG CAAATCTAAG 
ACTAAGCTCA ACACCATCAC TCTAGTGGGT AAGAAAAGGA AAAGAAACCC GTCTTCTGAT 
CACTCAGACG TAGATCTCAG CCCACCTGTT TCGCCCCGCA CACTGGAGGA AGAAATGTCA 
CAGAAGAGAC GTTCAAACCG GCAAGTGAAA CGGAAGAAGT ACACTGAGGA TTTGGACATT 
AAGATCACAG ATGATGAAGA TGAATTGGAT GCAGATGTTG ATGTGACCAC AACTCCTATG 
CCCGCAGTAG GGCATGTCCA GCCTTTGGGA GCAGAGCTCC CCCCGGAGCT GGATGGGGAT 
GGTCTTCCAA GCATGCAGTT TTTTGTGGAG AACCCAAGTG AGGAGGATGC TGCTATTGTG 
GATAAAATAT TGTCTATGCG GGTGACCAAA AAGGAGGTGT GTCCAGGGCA GTATACTAAT 
GTAGAGGAGT TTTTTGTCAA ATATAAAAAC TATTCGTACA TGCACTGTGA ATGGGCCAGT 
CTTGAGCAGC TGGAAAGAGA CAAGAGGATC CATCAGAAGC TTAAGAGATT CAAGACGAAA 
CAGGCACAAA TGAGGAACCT GTTCCAAGAG GATGAGGAAC CTTTCAATCC AGATTATGTG 
GAAGTTGATC GTATTTTGGA CGAGTCACAT AGTGTTGATA AAGACAACGG GGAGCCAGTT 
GTGTACTACC TGGTGAAGTG GTGCTCTCTA CCCTATGAAG ATGCTACTTG GGAGCTGAAG 
GAGGATGTTG ATGAAGGCAA AGTGGAAGAA TTCAGGAAGA TTGAAAGCCG CCAACCACGA 
CTCAAGAGAA CTCCTCGGCC TGCTGCAAGT GCCTGGAAAA AGCTAGATGA GTCCACCGAG 
TATAAGAATG GGAACCAGCT CAGAGAATAC CAGCTTGAGG GAGTCAACTG GCTGCTCTTT 
AACTGGTACA ACAGGCAAAA CTGCATCCTA GCGGATGAAA TGGGTCTGGG GAAGACAATT 
CAGTCCATTG CCCTGCTGTC AGAGATGTTT TCTGCGGGGG TCCAGAGCCC GTTCATGATC 
ATTGCCCCTC TGTCCACGAT CACAAACTGG GAAAGAGAGT TTTCGAACTG GACTGATATG 
AATGCTATTG TTTATCACGG CAGTCTGGCT AGTAGACAGA TGATCCAGCA GTATGAAATG 
TACTGCAAAG ATGACAAGGG ACACTTGATA CCAGGGGCCT ATAAGTTTGA CGCTCTGATC 
ACAACCTTTG AGATGATTCT ATCAGACTGC CCAGAGCTGA GGGAGATTTC CTGGCGCTGT 
GTAGTTATTG ATGAAGCTCA CCGTTTGAAG AACAGAAACT GCAAACTCCT CGACAGTCTC 
AAGATGCTTG AAATTGAGCA TAAAGTGTTG CTTACTGGCA CTCCACTACA AAACACAGTA 
GAGGAGCTCT TCAGTCTGCT GCACTTTCTG GAACCGGCCC AGTTTCCCTC AGAGATAGAG 
TTCTTGCGAG AGTTTGGAGA TCTTAAAACT GAGGAGCAGG TGCAGAAACT ACAGTCAATC 
CTGAAGCCCA TGATGCTGCG CAGACTGAAG GAAGATGTAG AAAAGAACCT TGCCCCCAAG 
CAAGAAACTA TTATTGAGGT GGAGTTGACC GATGTACAGA AAAAGTATTA CCGTGCTATT 
CTGGAGAGAA ACTTCAGCTT CCTTAGCATG GGAGCCACAC AAAACAGCAA TGTACCCAAT 
CTCCTCAACA CAATGATGGA ACTTCGAAAG TGCTGCAACC ACCCATACCT CATTACAGGT 
GCTGAGGAGA AGATCGTGTC TGAGCTGAGG GAGGTGTATG ATCCTCTGGC TCCGGACTTT 
CATCTCCAGG CTCTGGTGCG CTCTGCAGGG AAACTGGTCT TGTTGGATAA GCTGCTTCCT 
CGCCTCAAGG CTGGCGGGCA CAAGGTGCTC ATCTTCTCCC AGATGGTGAG GTGCCTTGAC 
ATCCTTGAGG ACTACCTCAT ACACAAGAGA TACCTGTATG AGCGCATTGA TGGACGGGTC 
AGAGGAAACC TGCGACAGGC GGCCATTGAT CGCTTCAGTA AACCTGATTC AGACCGGTTT 
GTCTTCCTGC TTTGTACTCG GGCTGGAGGT TTGGGCATTA ATCTGACCGC TGCTGACACC 
TGTGTCATTT TTGACTCTGA CTGGAACCCG CAGAATGATC TTCAAGCTCA GGCACGTTGC 
CATCGTATTG GTCAGTCTAA AGCGGTAAAG GTGTATCGTC TCATTACCAG AAACTCCTAC 
GAGAGGGAGA TGTTGGACAA AGCTAGTCTT AAACTGGGGC TTGACAGAGC CGTTCTGCAG 
AGCATGAGTG GAAACAAGGA AAGCAGTGTC AACGGGATCC AGCAGTTCTC TAAGAAGGAG 
ATTGAGGATT TGCTTCGTAA AGGTGCTTAT GCAGCCATAA TGGATGAGAA TGACGAGGGC 
AGTCGTTTCT GTGAAGAGGA CATCGACCAG ATCCTTCAGA GACGAGCAAC TACCATCACA 
ATAGAGAGCG AAGGCAAAGG ATCCACTTTT TCCAAGGCTA GCTTTGTGGC GTCTGAGAAC 
CGCACAGATA TCGCTCTAGA TGATCCCGAG TTCTGGCAGA AGTGGGCTAA GAAAGCAGAC 
ATAGACATGG ATTCTCTCAA CAGGAAGAAT ACTCTTGTCA TCGACACACC AAGAGTAAGA 
AAGCAAACAC GTCAGTTTTC CAGCCTGCGT GGAGAGGGTG GTGATCTCTC TGACTTGGAC 
AGCGATGATG ACTACCCACC CCATAATTCC CGTCAATCCA GAGCCTCCAG ACGCTCTGAC 
CGGCATTCTG GAGGAGGCTA TGGTCGCACA GACTGTTTCC GAGTGGAGAA ACATCTACTC 
GTTTATGGGT GGGGTCGTTG GCGAGACATC CTGTCGCATG CTCGCTGTAA GCGGCGTCTC 
AGTGAACGGG ACGTAGAAAC CATCTGCCGT GTCATCCTGG TTTTCTGTCT GATTCATTAT 
CGTGGAGATG AGAACATCAA GAGCTTCATT TGGGAGCTGA TTACCCCTCC AGAGAATGGA 
CGAGAACCAC AAGCTCTGCT CAATCACTCT GGTCTGTCCA TCCCTGTTCC TCGCGGCCGA 
AAGGGAAAGA GAGTGAAGGC TCAGAGCTCT TTTGACGTGC AGAAGGTTGA GTGGATCCGC 
AAATACAATC CTGACAGTCT GCTGCTGGAT GACAGTTACA GAAAACACCT CAAACACCAG 
TGCAACAAGG TTTTGCTGAG AGTACGTATG CTTTATTATC TGAAGCAGGA GGTCATTGGA 
GAACATGCTG ATTCTGTATT GAGTGGAGCT GATGCAAGGG ATATTGATAT CTGGTTGCCT 
GAAATGGAGC AGCAGGATGT TCCATCTGGC TGGTGGGATG CAGAAGCTGA CCGATGTTTG 
CTTATCGGAG TCTACAAGCA TGGATATGAG ATGTACACCA CCATGCGTGC AGACCCCTGC 
TTGTGTTTTG TTGAGCGATG TGGTCGTCCA AATGAGCAGG ACATTAATGC AGAGCAGCAA 
GCAGCAGACC CAGAGCTTGG AGAGGGAGGG GACTACGACA AGTACTCTGA AGATCCAGAG 
TTCAAACCCG CAACGAGACA TGCCAAGGAG ATGTATGAGG AGGGTGATTC GGTGAATGCA 
GATGGTGAGA TTTGTGTGGA GGATCGATCT GCCCCTATGC AAGTTGAAGG ACCGTCTTCG 
GGATCATCTG ATTTGTGTTA CTGGCCCACC AGCTCATCGC TCACAGCCCG ACTGCGACGT 
CTTATCACAG CTTACCAGCG CAGTTACAGA CGAGAGCAGC TTAAGATCGA GGCAGCAGAG 
AAGGGGGACC GTAGGCGAAG ACGCTGCGAA CAAGCTACCA AACTCAAAGA GATAGCCAGA 
CAAGAGCGAC AGCAACGGTG GACTCGTAGA GAAGAGTGCG ACTTTTACAG GGTGGTATCG 
ACATTTGGGG TGGAAAGGAT AAAGAAAGAA ACAGATGCTC CAGAAGGGGA CGAGCATCAC 
ATGGACTGGA ATCGTTTCCG TTCTTTTGCT CGACTCGATA AGAAAACCGA TGAGAGCTTG 
ACACGTTACT TTAAGTGCTT CATGTCCATG TGTCGGAAAG TGTGTCACAT TCGGCCAGGA 
CGTGGAGACG AATCTCAAGA TATGTCTCAG TCTCTGGCCC CGATCACAGA AGAGCGAGCC 
TCACGAACTC TCTACAGGGT CACCCTGCTG TGTCGGTTGC GTGAGCGTGT TCTTCCTCAC 
CCCTCGCTCG AGGAACGCCT CAGTCTGGCA CCACAAACCT CTGACCTGCC CAGTTGGTGG 
AGCATCCCAA AGCATGACCA CGAGCTTCTC TTAGCTGCTG CCAGACATGG AGTGAGCCGC 
ACTGAACTCT CCATCTTCTC TGACCCACTG TACTCCTTCA GTCAGAGCCG CCTCGACTAC 
CTCCAGAACC AGCAGGCTCA AGCAGCTGCA CAAATCCACG CCTTCAGTCA GTCACAGGAT 
CCAGCCGGCA TCAAAGAAGA GGGCCTGGAG GATGAGTCTC GACTGCTGGG TGTGGAGGCT 
CTCTGTCCGT CAGACTCTCC GGCCATGCTG CTCTCTCATT CTGACAGCAA AGTTGGCATT 
CAGGCTGGCT GGGTCTGGAA GAAGAGCAAA AACAACGGGC CGAGTGAGAG GAAATTGGGA 
GGGGGAGGAG GAGGGGCTTC AGACTCTGAT TCTGATTCAG ATTCAGGCTC GTCTTCCTCC 
TCTCGGCATT CAGGCAGCTC AGATGATAGT GGAGACAGTG ATGTGGAGAG AGAACAAGCA 
GCTCTTAAGA TGTGTGATGG TGATGAAGAA AACAGCATCC TCTCACTCAC GCCGTCACAG 
GAAGGAGCTC CACCTGAGTC CCTCACTGAC CCTCTAAGAG TGGACTGGCC CAAAGACCGC 
ATTCTGATCA ACAGGATAGA AAACCTCTGT TCACTGGTTA TTACGGGACA CTGGCCGTCA 
GGTCGCCGCT ACATCTCTGA CATCCAACTT AACACTGTAT CTGATGAGCA TGAACTCGGA 
GATGACCTCG GTTACTCACG GGTGGCACGA AAAATCAACA GTACCTTATC TGCAGAGGCT 
CTCGAAGGCC AGGAGTCAGA GTTTACAGTA AAGCTCCTAA AGGAGGAAGG CCTAAAGCTG 
ACCTTCTCCA AGCAGGCTCT GATGCCAAAC GGTGAAGGGA GTGCACGCAA AAAGCGAAAG 
GACCATGAGC TGGAAGATGC AGAAGGGGTT CTCCACGCTC CCCGCAGGAG GGACCTTCCC 
AACTGGCTCA AAGAAAACCC AGATTATGAG GTTGAGGGAG ATATGCTAGA ATTACTGGTG 
AACCGGACCA AAAGGAAGAG GAGGAGGAAG AGGGTGGAGA AAGGAGCAGC ACTTACTGGC 
AGCGAGAGAG TGAAGGTCAT AGACATACGG ACAGGCAAGA AGTTTGCTGG AGTGTTCGGG 
CCTGCACTAC AGGACCTGAG AGAGCACCTG GAGGAGAATC CTGACCACGC TGTAGCACCG 
GAATGGTCTG AGACTGTTCG CCACTCGGGC TTCTTGCCTG AGATTTTATT CCACAGACTG 
TTGAGTCCCC ACGCTTCAAT CCCCAAAAAG AGCCGGCATT ACCTCCACAC CCCTTCCCTT 
CAGACCGACG ACCCTCTCCT GGGAGGCGGT GAAGGTGAGA TGCTAGTTTC TGACGGCGCC 
TACATGATGG ACGATGAAGA CCTGGAGGAC GGAGGTCACC TCACGTCATC TCACCACTTC 
CTCACTCCAG CCTATGATGT GAAAATGGAA CCGAGCGCGC TCGATATGGA CGGAGGCGAC 
AGTTTGTCAC AGGGCGGTTA TGATAGCTCT GACAGAGAGG CCATTTTGGA TGATGTCATC 
ATGGCACCCA AACATTCGGA CACTTCCTCG AGCTCAGAGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CHD8short, a naturally-occurring truncated form of a chromatin remodeler lacking the helicase domain, is a potent transcriptional coregulator.
Gene641303-309(2018 Jan)
Kunkel GR,Tracy JA,Jalufka FL,Lekven AC

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Human molecular genetics25(16)3539-3554(2016 08)
Asad Z,Pandey A,Babu A,Sun Y,Shevade K,Kapoor S,Ullah I,Ranjan S,Scaria V,Bajpai R,Sachidanandan C

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Characterization of ?? T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity.
Frontiers in immunology7675(2016)
Wan F,Hu CB,Ma JX,Gao K,Xiang LX,Shao JZ

CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors.
Proceedings of the National Academy of Sciences of the United States of America111(42)E4468-77(2014 Oct)
Sugathan A,Biagioli M,Golzio C,Erdin S,Blumenthal I,Manavalan P,Ragavendran A,Brand H,Lucente D,Miles J,Sheridan SD,Stortchevoi A,Kellis M,Haggarty SJ,Katsanis N,Gusella JF,Talkowski ME

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CHD8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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