Nek10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek10
Entrez Gene ID	674895
Full Name	NIMA (never in mitosis gene a)- related kinase 10
Synonyms	Gm282
General protein information	Preferred Names NIMA (never in mitosis gene a)- related kinase 10 Names serine/threonine-protein kinase Nek10 nek10 never in mitosis A-related kinase 10 nimA-related protein kinase 10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 A1-A2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006518092.2	XP_006518155.1	serine/threonine-protein kinase Nek10 isoform X1
XM_006518096.4	XP_006518159.1	serine/threonine-protein kinase Nek10 isoform X2
XM_017316165.1	XP_017171654.1	serine/threonine-protein kinase Nek10 isoform X3
XM_011244771.1	XP_011243073.1	serine/threonine-protein kinase Nek10 isoform X1
XM_006518096.3	XP_006518159.1	serine/threonine-protein kinase Nek10 isoform X2
NM_001195229.1	NP_001182158.1	serine/threonine-protein kinase Nek10

Pathways from BioSystems

Homologies

	NEK10	XP_001094156.2
Canis lupus familiaris (dog)	NEK10	XP_005634398.1
Mus musculus (house mouse)	Nek10	NP_001182158.1
Caenorhabditis elegans (roundworm)	nekl-4	NP_498178.3
Bos taurus (cattle)	LOC522335	XP_005222634.1
Rattus norvegicus (Norway rat)	Nek10	XP_006251783.1
Gallus gallus (chicken)	NEK10	XP_001235144.3
Danio rerio (zebrafish)	nek10	XP_002665088.2
Xenopus tropicalis (tropical clawed frog)	nek10	XP_002932455.2

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Lineage-specific biology revealed by a finished genome assembly of the mouse.
Church DM, Goodstadt L, Hillier LW, Zody MC, Goldstein S, She X, Bult CJ, Agarwala R, Cherry JL, DiCuccio M, Hlavina W, Kapustin Y, Meric P, Maglott D, Birtle Z, Marques AC, Graves T, Zhou S, Teague B, Potamousis K, Churas C, Place M, Herschleb J, Runnheim R, Forrest D, Amos-Landgraf J, Schwartz DC, Cheng Z, Lindblad-Toh K, Eichler EE, Ponting CP,
PLoS biology7(5)e1000112(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nek10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78839	XM_017316165.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu49343	XM_006518096.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu01630	NM_001195229.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$657.30 $939.00
OMu49340	XM_011244771.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu49340	XM_006518092.2 Latest version!	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu49343	XM_006518096.3	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek10 ( XM_017316165.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171654.1 Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu78839
Clone ID Related Accession (Same CDS sequence)	XM_017316165.1
Accession Version	XM_017316165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTACC ATGTCTCATA TTTTGAGTTC CTAGTTGAAC TACGTTCTAC GAGCCCAGAG 
GTCCTGAAGA GTGAGCCATA TGGAGAGAAG GCCGATGTCT GGGCTGCTGG CTGCATCCTT 
TATCAGATGG CGACTCTAAG TCCTCCCTTC TGTAGCACCA ACATGCTCTC CTTGGCTACT 
AAAATAGTGG AGGCTGTATA TGAGCCAGTG CCAGAAGGTA TCTACTCTGA AAAAGTGACA 
GATACCATTA GAAGGTGCCT CACTCCTGAT GCAGAAGCCC GTCCAGATAT TGTAGAAGTT 
AGTTCCATGA TATCAGATGT CATGATGAAG TATTTAGACA GATTATCCAC ATCCCAGCTA 
GCCTTGGAGA GGAAACTGGA AAGGGAGCGG AGGCGCACAC AGAGATACTT TATGGAAGCC 
AACAGGAATG CTGTCACATG TCACCATGAG CTGGCTCTTC TGTCCCAGGA GACCTTTGAG 
AAGGCAAGCC TGAGCAGCAG TAGCAGCGGG GCAGCCAGCC TGAAAAGTGA GCTTTCAGAG 
AGCGCAGAGC TGCCTGGAGA GGGCTGCCAC ATCCCCTGCG GGAAGGAGGA GGACAGGGTC 
TGTGAGGAGG TGCTGTCAGA AGACAACTTC CAGCTGGAGA GTGTGGAGAA AGACCTGTAT 
TCTGAGCTAG ACGATGAGCT GGACGTCTCG GACAACTGTA GCAGCTCCAG TTCAAGCCCC 
CTAAAGGAGT CTACATTCAG CATTTTAAAG CGAAGTTTCA GTGCTTCGGG AAGAGAAAGG 
CACTCACAGG CAAGGGACTT CATTGCTGGA CTAGGATCAA GACCAAGACC AGCATCTGCA 
GGCATCGCTG TATCCCAGCG GAAAGTGCGG CAGATCTGCG ACCCCATTCA GCAGATCCTG 
ATCCAGCTAC ATAAAGTTAT CTACATCACG CAGCTCCCTC CAGCTCTGCA CCATGATCTG 
AAGAGAAGGG TTATAGAGAG ATTCAAGAAG TCCCTGTTCA GTCAGCAGAG CAACCCATGT 
AACCTGAAAT CTGAAATTAA GAAGTTGTCT CAGGGGTCTC CAGAACCCAT CGAGCTGAAC 
TTTCTTACCT CAGATTATCA TTCACTTCGC CATTCACGAG CTGCAAACAA CTGGTCCCCA 
AGTGACCCCA CAGGTTCACC CAGCAGCTTT GAAGTGGAAG AGGGAGTCAC ATATGAGCAG 
ATGCAGACTG TGATTGAAGA AGTCCTAGAG GAAAGTGGCT ATTACAACTT TACAACTAAG 
AGATCTTCTA GGTGTCACTC TTTTCCATGG GTGACCAAGA GCTACACAGC CAAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171654.1
CDS	80..1399
Translation	MHYHVSYFEFLVELRSTSPEVLKSEPYGEKADVWAAGCILYQMATLSPPFCSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTIRRCLTPDAEARPDIVEVSSMISDVMMKYLDRLSTSQLALERKLERERRRTQRYFMEANRNAVTCHHELALLSQETFEKASLSSSSSGAASLKSELSESAELPGEGCHIPCGKEEDRVCEEVLSEDNFQLESVEKDLYSELDDELDVSDNCSSSSSSPLKESTFSILKRSFSASGRERHSQARDFIAGLGSRPRPASAGIAVSQRKVRQICDPIQQILIQLHKVIYITQLPPALHHDLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIELNFLTSDYHSLRHSRAANNWSPSDPTGSPSSFEVEEGVTYEQMQTVIEEVLEESGYYNFTTKRSSRCHSFPWVTKSYTAKR

Target ORF information:

RefSeq Version	XM_017316165.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316165.1

ORF Insert Sequence:

ATGCATTACC ATGTCTCATA TTTTGAGTTC CTAGTTGAAC TACGTTCTAC GAGCCCAGAG 
GTCCTGAAGA GTGAGCCATA TGGAGAGAAG GCCGATGTCT GGGCTGCTGG CTGCATCCTT 
TATCAGATGG CGACTCTAAG TCCTCCCTTC TGTAGCACCA ACATGCTCTC CTTGGCTACT 
AAAATAGTGG AGGCTGTATA TGAGCCAGTG CCAGAAGGTA TCTACTCTGA AAAAGTGACA 
GATACCATTA GAAGGTGCCT CACTCCTGAT GCAGAAGCCC GTCCAGATAT TGTAGAAGTT 
AGTTCCATGA TATCAGATGT CATGATGAAG TATTTAGACA GATTATCCAC ATCCCAGCTA 
GCCTTGGAGA GGAAACTGGA AAGGGAGCGG AGGCGCACAC AGAGATACTT TATGGAAGCC 
AACAGGAATG CTGTCACATG TCACCATGAG CTGGCTCTTC TGTCCCAGGA GACCTTTGAG 
AAGGCAAGCC TGAGCAGCAG TAGCAGCGGG GCAGCCAGCC TGAAAAGTGA GCTTTCAGAG 
AGCGCAGAGC TGCCTGGAGA GGGCTGCCAC ATCCCCTGCG GGAAGGAGGA GGACAGGGTC 
TGTGAGGAGG TGCTGTCAGA AGACAACTTC CAGCTGGAGA GTGTGGAGAA AGACCTGTAT 
TCTGAGCTAG ACGATGAGCT GGACGTCTCG GACAACTGTA GCAGCTCCAG TTCAAGCCCC 
CTAAAGGAGT CTACATTCAG CATTTTAAAG CGAAGTTTCA GTGCTTCGGG AAGAGAAAGG 
CACTCACAGG CAAGGGACTT CATTGCTGGA CTAGGATCAA GACCAAGACC AGCATCTGCA 
GGCATCGCTG TATCCCAGCG GAAAGTGCGG CAGATCTGCG ACCCCATTCA GCAGATCCTG 
ATCCAGCTAC ATAAAGTTAT CTACATCACG CAGCTCCCTC CAGCTCTGCA CCATGATCTG 
AAGAGAAGGG TTATAGAGAG ATTCAAGAAG TCCCTGTTCA GTCAGCAGAG CAACCCATGT 
AACCTGAAAT CTGAAATTAA GAAGTTGTCT CAGGGGTCTC CAGAACCCAT CGAGCTGAAC 
TTTCTTACCT CAGATTATCA TTCACTTCGC CATTCACGAG CTGCAAACAA CTGGTCCCCA 
AGTGACCCCA CAGGTTCACC CAGCAGCTTT GAAGTGGAAG AGGGAGTCAC ATATGAGCAG 
ATGCAGACTG TGATTGAAGA AGTCCTAGAG GAAAGTGGCT ATTACAACTT TACAACTAAG 
AGATCTTCTA GGTGTCACTC TTTTCCATGG GTGACCAAGA GCTACACAGC CAAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek10 ( XM_006518096.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518159.1 Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu49343
Clone ID Related Accession (Same CDS sequence)	XM_006518096.4 , XM_006518096.3
Accession Version	XM_006518096.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACCCAGA GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518159.1
CDS	689..2716
Translation	MPDQDTKAKSTEKTADKQQGTTTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNNMTKSEPTIRTGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQHQLFQEIFTALVRNRLICREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHKLGGIEDLAQYMEIVANEYLGYAEEQHCVDKLVNMTYIFQKLAAVKDQREWVTASGAHKTLVSLLGARDTTVLLGALLALASLAESSECREKISELNVVENLLMILHEYDLLSKRLTAELLRLLCAEPQIKEQVKLYEGIPILLSLLHSDHLKLLWSVIWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPGEIEENTVSLQAACCAALTELALNDTNAHQVVQENGVYTIAKLILPNKQSNAAQTNLLQCYAFRTLRFLFSMERNRPLFKRLFPTDLFETFIDIGHYVRDIGAYKDLVSQLNLLLEDELKQIAENIESINQKKAPLKYIGDYAVLDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGAPLGEHFNSLKEKHHHFSEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTQRS

Target ORF information:

RefSeq Version	XM_006518096.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518096.3

ORF Insert Sequence:

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACCCAGA GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek10 ( XM_006518096.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518159.1 Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu49343
Clone ID Related Accession (Same CDS sequence)	XM_006518096.4 , XM_006518096.3
Accession Version	XM_006518096.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518096.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACCCAGA GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518159.1
CDS	689..2716
Translation	MPDQDTKAKSTEKTADKQQGTTTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNNMTKSEPTIRTGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQHQLFQEIFTALVRNRLICREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHKLGGIEDLAQYMEIVANEYLGYAEEQHCVDKLVNMTYIFQKLAAVKDQREWVTASGAHKTLVSLLGARDTTVLLGALLALASLAESSECREKISELNVVENLLMILHEYDLLSKRLTAELLRLLCAEPQIKEQVKLYEGIPILLSLLHSDHLKLLWSVIWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPGEIEENTVSLQAACCAALTELALNDTNAHQVVQENGVYTIAKLILPNKQSNAAQTNLLQCYAFRTLRFLFSMERNRPLFKRLFPTDLFETFIDIGHYVRDIGAYKDLVSQLNLLLEDELKQIAENIESINQKKAPLKYIGDYAVLDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGAPLGEHFNSLKEKHHHFSEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTQRS

Target ORF information:

RefSeq Version	XM_006518096.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518096.4

ORF Insert Sequence:

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACCCAGA GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek10 ( NM_001195229.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001182158.1 Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), mRNA.

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CloneID	OMu01630
Clone ID Related Accession (Same CDS sequence)	NM_001195229.1
Accession Version	NM_001195229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129580.14, AK147862.1 and AC117608.13. On or before Dec 3, 2011 this sequence version replaced XM_003085000.1, XM_003085898.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This exon combination is predicted due to lack of full-length transcript support; the genomic coordinates used for the 5' and 3' exons are based on homologous human transcript alignments from GeneID:152110. It is not known if this exon combination exists in mouse. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849384, SAMN01164131 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAGGAAAGCA GTAAGCTTAC ATCTATGGTG GGAACGATCC TCTATTCTTG CCCAGAGGTC 
CTGAAGAGTG AGCCATATGG AGAGAAGGCC GATGTCTGGG CTGCTGGCTG CATCCTTTAT 
CAGATGGCGA CTCTAAGTCC TCCCTTCTGT AGCACCAACA TGCTCTCCTT GGCTACTAAA 
ATAGTGGAGG CTGTATATGA GCCAGTGCCA GAAGGTATCT ACTCTGAAAA AGTGACAGAT 
ACCATTAGAA GGTGCCTCAC TCCTGATGCA GAAGCCCGTC CAGATATTGT AGAAGTTAGT 
TCCATGATAT CAGATGTCAT GATGAAGTAT TTAGACAGAT TATCCACATC CCAGCTAGCC 
TTGGAGAGGA AACTGGAAAG GGAGCGGAGG CGCACACAGA GATACTTTAT GGAAGCCAAC 
AGGAATGCTG TCACATGTCA CCATGAGCTG GCTCTTCTGT CCCAGGAGAC CTTTGAGAAG 
GCAAGCCTGA GCAGCAGTAG CAGCGGGGCA GCCAGCCTGA AAAGTGAGCT TTCAGAGAGC 
GCAGAGCTGC CTGGAGAGGG CTGCCACATC CCCTGCGGGA AGGAGGAGGA CAGGGTCTGT 
GAGGAGGTGC TGTCAGAAGA CAACTTCCAG CTGGAGAGTG TGGAGAAAGA CCTGTATTCT 
GAGCTAGACG ATGAGCTGGA CGTCTCGGAC AACTGTAGCA GCTCCAGTTC AAGCCCCCTA 
AAGGAGTCTA CATTCAGCAT TTTAAAGCGA AGTTTCAGTG CTTCGGGAAG AGAAAGGCAC 
TCACAGGCAA GGGACTTCAT TGCTGGACTA GGATCAAGAC CAAGACCAGC ATCTGCAGGC 
ATCGCTGTAT CCCAGCGGAA AGTGCGGCAG ATCTGCGACC CCATTCAGCA GATCCTGATC 
CAGCTACATA AAGTTATCTA CATCACGCAG CTCCCTCCAG CTCTGCACCA TGATCTGAAG 
AGAAGGGTTA TAGAGAGATT CAAGAAGTCC CTGTTCAGTC AGCAGAGCAA CCCATGTAAC 
CTGAAATCTG AAATTAAGAA GTTGTCTCAG GGGTCTCCAG AACCCATCGA GCTGAACTTT 
CTTACCTCAG ATTATCATTC ACTTCGCCAT TCACGAGCTG CAAACAACTG GTCCCCAAGT 
GACCCCACAG GTTCACCCAG CAGCTTTGAA GTGGAAGAGG GAGTCACATA TGAGCAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGCTATT ACAACTTTAC AACTAAGAGG 
TGTCACTCTT TTCCATGGGT GACCAAGAGC TACACAGCCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182158.1
CDS	1..3348
Translation	MPDQDTKAKSTEKTADKQQGTTTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNNMTKSEPTIRTGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQHQLFQEIFTALVRNRLICREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHKLGGIEDLAQYMEIVANEYLGYAEEQHCVDKLVNMTYIFQKLAAVKDQREWVTASGAHKTLVSLLGARDTTVLLGALLALASLAESSECREKISELNVVENLLMILHEYDLLSKRLTAELLRLLCAEPQIKEQVKLYEGIPILLSLLHSDHLKLLWSVIWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPGEIEENTVSLQAACCAALTELALNDTNAHQVVQENGVYTIAKLILPNKQSNAAQTNLLQCYAFRTLRFLFSMERNRPLFKRLFPTDLFETFIDIGHYVRDIGAYKDLVSQLNLLLEDELKQIAENIESINQKKAPLKYIGDYAVLDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGAPLGEHFNSLKEKHHHFSEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQESSKLTSMVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLSPPFCSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTIRRCLTPDAEARPDIVEVSSMISDVMMKYLDRLSTSQLALERKLERERRRTQRYFMEANRNAVTCHHELALLSQETFEKASLSSSSSGAASLKSELSESAELPGEGCHIPCGKEEDRVCEEVLSEDNFQLESVEKDLYSELDDELDVSDNCSSSSSSPLKESTFSILKRSFSASGRERHSQARDFIAGLGSRPRPASAGIAVSQRKVRQICDPIQQILIQLHKVIYITQLPPALHHDLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIELNFLTSDYHSLRHSRAANNWSPSDPTGSPSSFEVEEGVTYEQMQTVIEEVLEESGYYNFTTKRCHSFPWVTKSYTAKR

Target ORF information:

RefSeq Version	NM_001195229.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195229.1

ORF Insert Sequence:

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAGGAAAGCA GTAAGCTTAC ATCTATGGTG GGAACGATCC TCTATTCTTG CCCAGAGGTC 
CTGAAGAGTG AGCCATATGG AGAGAAGGCC GATGTCTGGG CTGCTGGCTG CATCCTTTAT 
CAGATGGCGA CTCTAAGTCC TCCCTTCTGT AGCACCAACA TGCTCTCCTT GGCTACTAAA 
ATAGTGGAGG CTGTATATGA GCCAGTGCCA GAAGGTATCT ACTCTGAAAA AGTGACAGAT 
ACCATTAGAA GGTGCCTCAC TCCTGATGCA GAAGCCCGTC CAGATATTGT AGAAGTTAGT 
TCCATGATAT CAGATGTCAT GATGAAGTAT TTAGACAGAT TATCCACATC CCAGCTAGCC 
TTGGAGAGGA AACTGGAAAG GGAGCGGAGG CGCACACAGA GATACTTTAT GGAAGCCAAC 
AGGAATGCTG TCACATGTCA CCATGAGCTG GCTCTTCTGT CCCAGGAGAC CTTTGAGAAG 
GCAAGCCTGA GCAGCAGTAG CAGCGGGGCA GCCAGCCTGA AAAGTGAGCT TTCAGAGAGC 
GCAGAGCTGC CTGGAGAGGG CTGCCACATC CCCTGCGGGA AGGAGGAGGA CAGGGTCTGT 
GAGGAGGTGC TGTCAGAAGA CAACTTCCAG CTGGAGAGTG TGGAGAAAGA CCTGTATTCT 
GAGCTAGACG ATGAGCTGGA CGTCTCGGAC AACTGTAGCA GCTCCAGTTC AAGCCCCCTA 
AAGGAGTCTA CATTCAGCAT TTTAAAGCGA AGTTTCAGTG CTTCGGGAAG AGAAAGGCAC 
TCACAGGCAA GGGACTTCAT TGCTGGACTA GGATCAAGAC CAAGACCAGC ATCTGCAGGC 
ATCGCTGTAT CCCAGCGGAA AGTGCGGCAG ATCTGCGACC CCATTCAGCA GATCCTGATC 
CAGCTACATA AAGTTATCTA CATCACGCAG CTCCCTCCAG CTCTGCACCA TGATCTGAAG 
AGAAGGGTTA TAGAGAGATT CAAGAAGTCC CTGTTCAGTC AGCAGAGCAA CCCATGTAAC 
CTGAAATCTG AAATTAAGAA GTTGTCTCAG GGGTCTCCAG AACCCATCGA GCTGAACTTT 
CTTACCTCAG ATTATCATTC ACTTCGCCAT TCACGAGCTG CAAACAACTG GTCCCCAAGT 
GACCCCACAG GTTCACCCAG CAGCTTTGAA GTGGAAGAGG GAGTCACATA TGAGCAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGCTATT ACAACTTTAC AACTAAGAGG 
TGTCACTCTT TTCCATGGGT GACCAAGAGC TACACAGCCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek10 ( XM_006518092.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518155.1 Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X1, mRNA.

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CloneID	OMu49340
Clone ID Related Accession (Same CDS sequence)	XM_006518092.2 , XM_011244771.1
Accession Version	XM_006518092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006518092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAGGAAAGCA GTAAGCTTAC ATCTATGGTG GGAACGATCC TCTATTCTTG CCCAGAGGTC 
CTGAAGAGTG AGCCATATGG AGAGAAGGCC GATGTCTGGG CTGCTGGCTG CATCCTTTAT 
CAGATGGCGA CTCTAAGTCC TCCCTTCTGT AGCACCAACA TGCTCTCCTT GGCTACTAAA 
ATAGTGGAGG CTGTATATGA GCCAGTGCCA GAAGGTATCT ACTCTGAAAA AGTGACAGAT 
ACCATTAGAA GGTGCCTCAC TCCTGATGCA GAAGCCCGTC CAGATATTGT AGAAGTTAGT 
TCCATGATAT CAGATGTCAT GATGAAGTAT TTAGACAGAT TATCCACATC CCAGCTAGCC 
TTGGAGAGGA AACTGGAAAG GGAGCGGAGG CGCACACAGA GATACTTTAT GGAAGCCAAC 
AGGAATGCTG TCACATGTCA CCATGAGCTG GCTCTTCTGT CCCAGGAGAC CTTTGAGAAG 
GCAAGCCTGA GCAGCAGTAG CAGCGGGGCA GCCAGCCTGA AAAGTGAGCT TTCAGAGAGC 
GCAGAGCTGC CTGGAGAGGG CTGCCACATC CCCTGCGGGA AGGAGGAGGA CAGGGTCTGT 
GAGGAGGTGC TGTCAGAAGA CAACTTCCAG CTGGAGAGTG TGGAGAAAGA CCTGTATTCT 
GAGCTAGACG ATGAGCTGGA CGTCTCGGAC AACTGTAGCA GCTCCAGTTC AAGCCCCCTA 
AAGGAGTCTA CATTCAGCAT TTTAAAGCGA AGTTTCAGTG CTTCGGGAAG AGAAAGGCAC 
TCACAGGCAA GGGACTTCAT TGCTGGACTA GGATCAAGAC CAAGACCAGC ATCTGCAGGC 
ATCGCTGTAT CCCAGCGGAA AGTGCGGCAG ATCTGCGACC CCATTCAGCA GATCCTGATC 
CAGCTACATA AAGTTATCTA CATCACGCAG CTCCCTCCAG CTCTGCACCA TGATCTGAAG 
AGAAGGGTTA TAGAGAGATT CAAGAAGTCC CTGTTCAGTC AGCAGAGCAA CCCATGTAAC 
CTGAAATCTG AAATTAAGAA GTTGTCTCAG GGGTCTCCAG AACCCATCGA GCTGAACTTT 
CTTACCTCAG ATTATCATTC ACTTCGCCAT TCACGAGCTG CAAACAACTG GTCCCCAAGT 
GACCCCACAG GTTCACCCAG CAGCTTTGAA GTGGAAGAGG GAGTCACATA TGAGCAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGCTATT ACAACTTTAC AACTAAGAGA 
TCTTCTAGGT GTCACTCTTT TCCATGGGTG ACCAAGAGCT ACACAGCCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518155.1
CDS	689..4045
Translation	MPDQDTKAKSTEKTADKQQGTTTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNNMTKSEPTIRTGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQHQLFQEIFTALVRNRLICREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHKLGGIEDLAQYMEIVANEYLGYAEEQHCVDKLVNMTYIFQKLAAVKDQREWVTASGAHKTLVSLLGARDTTVLLGALLALASLAESSECREKISELNVVENLLMILHEYDLLSKRLTAELLRLLCAEPQIKEQVKLYEGIPILLSLLHSDHLKLLWSVIWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPGEIEENTVSLQAACCAALTELALNDTNAHQVVQENGVYTIAKLILPNKQSNAAQTNLLQCYAFRTLRFLFSMERNRPLFKRLFPTDLFETFIDIGHYVRDIGAYKDLVSQLNLLLEDELKQIAENIESINQKKAPLKYIGDYAVLDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGAPLGEHFNSLKEKHHHFSEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQESSKLTSMVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLSPPFCSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTIRRCLTPDAEARPDIVEVSSMISDVMMKYLDRLSTSQLALERKLERERRRTQRYFMEANRNAVTCHHELALLSQETFEKASLSSSSSGAASLKSELSESAELPGEGCHIPCGKEEDRVCEEVLSEDNFQLESVEKDLYSELDDELDVSDNCSSSSSSPLKESTFSILKRSFSASGRERHSQARDFIAGLGSRPRPASAGIAVSQRKVRQICDPIQQILIQLHKVIYITQLPPALHHDLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIELNFLTSDYHSLRHSRAANNWSPSDPTGSPSSFEVEEGVTYEQMQTVIEEVLEESGYYNFTTKRSSRCHSFPWVTKSYTAKR

Target ORF information:

RefSeq Version	XM_006518092.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518092.2

ORF Insert Sequence:

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAGGAAAGCA GTAAGCTTAC ATCTATGGTG GGAACGATCC TCTATTCTTG CCCAGAGGTC 
CTGAAGAGTG AGCCATATGG AGAGAAGGCC GATGTCTGGG CTGCTGGCTG CATCCTTTAT 
CAGATGGCGA CTCTAAGTCC TCCCTTCTGT AGCACCAACA TGCTCTCCTT GGCTACTAAA 
ATAGTGGAGG CTGTATATGA GCCAGTGCCA GAAGGTATCT ACTCTGAAAA AGTGACAGAT 
ACCATTAGAA GGTGCCTCAC TCCTGATGCA GAAGCCCGTC CAGATATTGT AGAAGTTAGT 
TCCATGATAT CAGATGTCAT GATGAAGTAT TTAGACAGAT TATCCACATC CCAGCTAGCC 
TTGGAGAGGA AACTGGAAAG GGAGCGGAGG CGCACACAGA GATACTTTAT GGAAGCCAAC 
AGGAATGCTG TCACATGTCA CCATGAGCTG GCTCTTCTGT CCCAGGAGAC CTTTGAGAAG 
GCAAGCCTGA GCAGCAGTAG CAGCGGGGCA GCCAGCCTGA AAAGTGAGCT TTCAGAGAGC 
GCAGAGCTGC CTGGAGAGGG CTGCCACATC CCCTGCGGGA AGGAGGAGGA CAGGGTCTGT 
GAGGAGGTGC TGTCAGAAGA CAACTTCCAG CTGGAGAGTG TGGAGAAAGA CCTGTATTCT 
GAGCTAGACG ATGAGCTGGA CGTCTCGGAC AACTGTAGCA GCTCCAGTTC AAGCCCCCTA 
AAGGAGTCTA CATTCAGCAT TTTAAAGCGA AGTTTCAGTG CTTCGGGAAG AGAAAGGCAC 
TCACAGGCAA GGGACTTCAT TGCTGGACTA GGATCAAGAC CAAGACCAGC ATCTGCAGGC 
ATCGCTGTAT CCCAGCGGAA AGTGCGGCAG ATCTGCGACC CCATTCAGCA GATCCTGATC 
CAGCTACATA AAGTTATCTA CATCACGCAG CTCCCTCCAG CTCTGCACCA TGATCTGAAG 
AGAAGGGTTA TAGAGAGATT CAAGAAGTCC CTGTTCAGTC AGCAGAGCAA CCCATGTAAC 
CTGAAATCTG AAATTAAGAA GTTGTCTCAG GGGTCTCCAG AACCCATCGA GCTGAACTTT 
CTTACCTCAG ATTATCATTC ACTTCGCCAT TCACGAGCTG CAAACAACTG GTCCCCAAGT 
GACCCCACAG GTTCACCCAG CAGCTTTGAA GTGGAAGAGG GAGTCACATA TGAGCAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGCTATT ACAACTTTAC AACTAAGAGA 
TCTTCTAGGT GTCACTCTTT TCCATGGGTG ACCAAGAGCT ACACAGCCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek10 ( XM_011244771.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243073.1 Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X2, mRNA.

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CloneID	OMu49340
Clone ID Related Accession (Same CDS sequence)	XM_006518092.2 , XM_011244771.1
Accession Version	XM_011244771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAGGAAAGCA GTAAGCTTAC ATCTATGGTG GGAACGATCC TCTATTCTTG CCCAGAGGTC 
CTGAAGAGTG AGCCATATGG AGAGAAGGCC GATGTCTGGG CTGCTGGCTG CATCCTTTAT 
CAGATGGCGA CTCTAAGTCC TCCCTTCTGT AGCACCAACA TGCTCTCCTT GGCTACTAAA 
ATAGTGGAGG CTGTATATGA GCCAGTGCCA GAAGGTATCT ACTCTGAAAA AGTGACAGAT 
ACCATTAGAA GGTGCCTCAC TCCTGATGCA GAAGCCCGTC CAGATATTGT AGAAGTTAGT 
TCCATGATAT CAGATGTCAT GATGAAGTAT TTAGACAGAT TATCCACATC CCAGCTAGCC 
TTGGAGAGGA AACTGGAAAG GGAGCGGAGG CGCACACAGA GATACTTTAT GGAAGCCAAC 
AGGAATGCTG TCACATGTCA CCATGAGCTG GCTCTTCTGT CCCAGGAGAC CTTTGAGAAG 
GCAAGCCTGA GCAGCAGTAG CAGCGGGGCA GCCAGCCTGA AAAGTGAGCT TTCAGAGAGC 
GCAGAGCTGC CTGGAGAGGG CTGCCACATC CCCTGCGGGA AGGAGGAGGA CAGGGTCTGT 
GAGGAGGTGC TGTCAGAAGA CAACTTCCAG CTGGAGAGTG TGGAGAAAGA CCTGTATTCT 
GAGCTAGACG ATGAGCTGGA CGTCTCGGAC AACTGTAGCA GCTCCAGTTC AAGCCCCCTA 
AAGGAGTCTA CATTCAGCAT TTTAAAGCGA AGTTTCAGTG CTTCGGGAAG AGAAAGGCAC 
TCACAGGCAA GGGACTTCAT TGCTGGACTA GGATCAAGAC CAAGACCAGC ATCTGCAGGC 
ATCGCTGTAT CCCAGCGGAA AGTGCGGCAG ATCTGCGACC CCATTCAGCA GATCCTGATC 
CAGCTACATA AAGTTATCTA CATCACGCAG CTCCCTCCAG CTCTGCACCA TGATCTGAAG 
AGAAGGGTTA TAGAGAGATT CAAGAAGTCC CTGTTCAGTC AGCAGAGCAA CCCATGTAAC 
CTGAAATCTG AAATTAAGAA GTTGTCTCAG GGGTCTCCAG AACCCATCGA GCTGAACTTT 
CTTACCTCAG ATTATCATTC ACTTCGCCAT TCACGAGCTG CAAACAACTG GTCCCCAAGT 
GACCCCACAG GTTCACCCAG CAGCTTTGAA GTGGAAGAGG GAGTCACATA TGAGCAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGCTATT ACAACTTTAC AACTAAGAGA 
TCTTCTAGGT GTCACTCTTT TCCATGGGTG ACCAAGAGCT ACACAGCCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243073.1
CDS	396..3752
Translation	MPDQDTKAKSTEKTADKQQGTTTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNNMTKSEPTIRTGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQHQLFQEIFTALVRNRLICREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHKLGGIEDLAQYMEIVANEYLGYAEEQHCVDKLVNMTYIFQKLAAVKDQREWVTASGAHKTLVSLLGARDTTVLLGALLALASLAESSECREKISELNVVENLLMILHEYDLLSKRLTAELLRLLCAEPQIKEQVKLYEGIPILLSLLHSDHLKLLWSVIWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPGEIEENTVSLQAACCAALTELALNDTNAHQVVQENGVYTIAKLILPNKQSNAAQTNLLQCYAFRTLRFLFSMERNRPLFKRLFPTDLFETFIDIGHYVRDIGAYKDLVSQLNLLLEDELKQIAENIESINQKKAPLKYIGDYAVLDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGAPLGEHFNSLKEKHHHFSEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQESSKLTSMVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLSPPFCSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTIRRCLTPDAEARPDIVEVSSMISDVMMKYLDRLSTSQLALERKLERERRRTQRYFMEANRNAVTCHHELALLSQETFEKASLSSSSSGAASLKSELSESAELPGEGCHIPCGKEEDRVCEEVLSEDNFQLESVEKDLYSELDDELDVSDNCSSSSSSPLKESTFSILKRSFSASGRERHSQARDFIAGLGSRPRPASAGIAVSQRKVRQICDPIQQILIQLHKVIYITQLPPALHHDLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIELNFLTSDYHSLRHSRAANNWSPSDPTGSPSSFEVEEGVTYEQMQTVIEEVLEESGYYNFTTKRSSRCHSFPWVTKSYTAKR

Target ORF information:

RefSeq Version	XM_011244771.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)- related kinase 10 (Nek10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244771.1

ORF Insert Sequence:

ATGCCCGATC AAGATACAAA GGCGAAGAGC ACAGAGAAAA CCGCTGATAA GCAACAAGGG 
ACCACCACCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTGTTGAA CGTTCAGTCA 
AGCAAACAGC AGCTTCCAGC CATTAACTTT GACAGTGCCC AAAATAACAT GACAAAGTCT 
GAGCCAACCA TCAGGACAGG GGGACATAGA GCTCGTGGTC AATGGCATGA GTCCACAGAA 
GCAGTTGAAC TTGAAAATTT TAGTATAAAC TATAAGAATG AAAGGAATTT CAGCAAACAT 
CCACAGCATC AGTTATTTCA AGAGATCTTC ACAGCTCTGG TGAGAAACAG ACTTATATGC 
AGAGAGTGGG TTAATCGTGC TCCATCCATT CATTTTCTGA GAGTGTTAAT TTGTCTGAGG 
CTGCTGATGC GGGATCCATG TTATCAGGAA ATACTCCATA AATTGGGTGG TATTGAAGAC 
CTTGCTCAGT ACATGGAGAT CGTGGCCAAC GAGTACCTGG GCTACGCTGA GGAGCAGCAC 
TGTGTGGACA AGCTGGTCAA TATGACGTAT ATTTTTCAAA AACTTGCTGC TGTGAAAGAC 
CAAAGAGAAT GGGTTACTGC AAGTGGAGCC CACAAGACAT TAGTGAGTTT GCTTGGTGCC 
CGAGACACCA CTGTCCTCTT GGGTGCTCTG CTGGCACTGG CCAGCTTAGC AGAGAGTTCA 
GAGTGTAGGG AGAAGATCAG TGAACTCAAC GTAGTAGAAA ATCTGTTGAT GATTCTGCAC 
GAATATGATT TACTCTCCAA AAGACTCACT GCCGAGCTCC TGCGCCTGCT TTGTGCTGAG 
CCCCAGATCA AGGAGCAGGT GAAACTCTAT GAAGGGATCC CCATCCTGCT CAGTCTGCTT 
CATTCTGATC ACTTGAAGCT GCTGTGGAGT GTGATCTGGA TTCTGGTCCA GGTGTGTGAG 
GACCCGGAGA CTAGTGTGGA AATCCGAATC TGGGGTGGCA TCAAGCAGCT CCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAC CGTTCTTCCA TCGGAAGCCT GTCCAGTGCA 
AATGCCGCAG GACGCATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC TGGGGAAATC 
GAGGAAAATA CTGTCTCTCT CCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGCCCTC 
AATGATACCA ATGCCCACCA AGTGGTACAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAACAATC GAATGCAGCA CAAACTAATC TACTACAGTG TTATGCTTTC 
AGAACCCTGA GGTTTCTCTT CAGTATGGAA AGGAACAGGC CACTCTTTAA AAGACTTTTT 
CCCACCGACC TGTTTGAGAC CTTCATTGAC ATAGGACATT ACGTTCGTGA TATTGGTGCT 
TATAAGGACT TGGTATCACA GTTGAATTTA CTATTGGAAG ATGAGCTGAA GCAAATTGCT 
GAGAATATTG AAAGCATCAA TCAGAAGAAA GCGCCCTTGA AGTACATAGG TGACTATGCT 
GTGTTGGACC ACCTTGGAAG TGGAGCTTTT GGCTGTGTTT ATAAGGTTAG AAAGCGTAGT 
GGTCAAAATC TTTTAGCAAT GAAGGAGGTC AATTTGCATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGACC GAGACAGCAG TGTCAAGAAC ATTGTTTCTG AATTAACAAT AATTAAGGAG 
CAGCTTTATC ATCCCAATGT TGTACGTTAT TATAAAACAT TTTTGGAAAA TGACAGGTTG 
TATATCGTTA TGGAGCTGAT AGAAGGAGCC CCACTTGGAG AGCACTTTAA TTCTTTGAAG 
GAAAAACATC ACCATTTCAG TGAAGAGCGA CTGTGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ATCTGCACAA GGAGAAGAGG ATTGTTCACA GAGATCTCAC GCCAAACAAC 
ATTATGCTGG GAGATAAGGA CAAAGTGACA GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAGGAAAGCA GTAAGCTTAC ATCTATGGTG GGAACGATCC TCTATTCTTG CCCAGAGGTC 
CTGAAGAGTG AGCCATATGG AGAGAAGGCC GATGTCTGGG CTGCTGGCTG CATCCTTTAT 
CAGATGGCGA CTCTAAGTCC TCCCTTCTGT AGCACCAACA TGCTCTCCTT GGCTACTAAA 
ATAGTGGAGG CTGTATATGA GCCAGTGCCA GAAGGTATCT ACTCTGAAAA AGTGACAGAT 
ACCATTAGAA GGTGCCTCAC TCCTGATGCA GAAGCCCGTC CAGATATTGT AGAAGTTAGT 
TCCATGATAT CAGATGTCAT GATGAAGTAT TTAGACAGAT TATCCACATC CCAGCTAGCC 
TTGGAGAGGA AACTGGAAAG GGAGCGGAGG CGCACACAGA GATACTTTAT GGAAGCCAAC 
AGGAATGCTG TCACATGTCA CCATGAGCTG GCTCTTCTGT CCCAGGAGAC CTTTGAGAAG 
GCAAGCCTGA GCAGCAGTAG CAGCGGGGCA GCCAGCCTGA AAAGTGAGCT TTCAGAGAGC 
GCAGAGCTGC CTGGAGAGGG CTGCCACATC CCCTGCGGGA AGGAGGAGGA CAGGGTCTGT 
GAGGAGGTGC TGTCAGAAGA CAACTTCCAG CTGGAGAGTG TGGAGAAAGA CCTGTATTCT 
GAGCTAGACG ATGAGCTGGA CGTCTCGGAC AACTGTAGCA GCTCCAGTTC AAGCCCCCTA 
AAGGAGTCTA CATTCAGCAT TTTAAAGCGA AGTTTCAGTG CTTCGGGAAG AGAAAGGCAC 
TCACAGGCAA GGGACTTCAT TGCTGGACTA GGATCAAGAC CAAGACCAGC ATCTGCAGGC 
ATCGCTGTAT CCCAGCGGAA AGTGCGGCAG ATCTGCGACC CCATTCAGCA GATCCTGATC 
CAGCTACATA AAGTTATCTA CATCACGCAG CTCCCTCCAG CTCTGCACCA TGATCTGAAG 
AGAAGGGTTA TAGAGAGATT CAAGAAGTCC CTGTTCAGTC AGCAGAGCAA CCCATGTAAC 
CTGAAATCTG AAATTAAGAA GTTGTCTCAG GGGTCTCCAG AACCCATCGA GCTGAACTTT 
CTTACCTCAG ATTATCATTC ACTTCGCCAT TCACGAGCTG CAAACAACTG GTCCCCAAGT 
GACCCCACAG GTTCACCCAG CAGCTTTGAA GTGGAAGAGG GAGTCACATA TGAGCAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGCTATT ACAACTTTAC AACTAAGAGA 
TCTTCTAGGT GTCACTCTTT TCCATGGGTG ACCAAGAGCT ACACAGCCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Lineage-specific biology revealed by a finished genome assembly of the mouse.
PLoS biology7(5)e1000112(2009 May)
Church DM,Goodstadt L,Hillier LW,Zody MC,Goldstein S,She X,Bult CJ,Agarwala R,Cherry JL,DiCuccio M,Hlavina W,Kapustin Y,Meric P,Maglott D,Birtle Z,Marques AC,Graves T,Zhou S,Teague B,Potamousis K,Churas C,Place M,Herschleb J,Runnheim R,Forrest D,Amos-Landgraf J,Schwartz DC,Cheng Z,Lindblad-Toh K,Eichler EE,Ponting CP,

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Nek10 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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