NEK10 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005634341.1	XP_005634398.1	serine/threonine-protein kinase Nek10 isoform X1
XM_005634345.1	XP_005634402.1	serine/threonine-protein kinase Nek10 isoform X5
XM_542757.7	XP_542757.3	serine/threonine-protein kinase Nek10 isoform X1
XM_005634342.1	XP_005634399.1	serine/threonine-protein kinase Nek10 isoform X2
XM_005634344.2	XP_005634401.1	serine/threonine-protein kinase Nek10 isoform X3
XM_005634343.1	XP_005634400.1	serine/threonine-protein kinase Nek10 isoform X3
XM_022408563.1	XP_022264271.1	serine/threonine-protein kinase Nek10 isoform X4
XM_005634345.2	XP_005634402.1	serine/threonine-protein kinase Nek10 isoform X5
XM_542757.6	XP_542757.3	serine/threonine-protein kinase Nek10 isoform X1
XM_005634344.1	XP_005634401.1	serine/threonine-protein kinase Nek10 isoform X4
XM_005634341.2	XP_005634398.1	serine/threonine-protein kinase Nek10 isoform X1
XM_005634342.2	XP_005634399.1	serine/threonine-protein kinase Nek10 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe296473	XM_022408563.1 Latest version!	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe24059	XM_005634341.2 Latest version!	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe24059	XM_542757.7 Latest version!	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe24060	XM_005634342.2 Latest version!	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe24061	XM_005634343.1 Latest version!	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe24062	XM_005634344.2 Latest version!	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe24063	XM_005634345.2 Latest version!	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe24063	XM_005634345.1	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe24059	XM_542757.6	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe24062	XM_005634344.1	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe24059	XM_005634341.1	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe24060	XM_005634342.1	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

NEK10 ( XM_022408563.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264271.1 Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe296473
Clone ID Related Accession (Same CDS sequence)	XM_022408563.1
Accession Version	XM_022408563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCAA GTATAAACTA CAAGAATGAG AGAAACTTCA GCAAACATCC CCAGCATAAA 
CTATTTCAGG AGATCTTTAC AGCCTTGGTG AAAAATAGAC TCATCTGCAG AGAGTGGGTT 
CATCGAGCCC CATCTATTCA TTTTCTGAGA GTGTTAATCT GTCTGAGACT TCTAATGAGG 
GATCCATGTT ATCAGGAAAT ACTTCATAGC TTGGGTGGAA TTGAAAACCT AGCTCAGTAC 
ATGGAGATTG TGGCCAATGA GTACCTCAGC TACGGGGAAG AGCAGCATAG TGTGGACAAG 
CTAGTCAACA TGACATATAT TTTTCAAAAA CTTGCTGCTG TCAAAGACCA AAGAGAATGG 
GTCACCACAA GCGGAGCCCA TAAGACATTA GTAAATTTAC TTGGTGCCCG AGATACTAAT 
GTTTTGTTGG GTGCCCTTCT GGCTCTGGCT AGTTTAGCTG AAAGTCCAGA ATGTAGGGAG 
AAGATAAGTG AACTCAACAT TGTAGAAAAT CTGCTGATGA TTTTACATGA ATATGACTTG 
CTTTCCAAAA GATTAACTGC GGAGTTACTA CGCCTACTTT GTGCCGAACC CCAGGTGAAA 
GAGCAGGTGA AGCTCTATGA GGGGATACCC ATCCTCCTCA GTCTGCTCCA CTCTGACCAC 
CTGAAGCTGC TCTGGAGTGT TGTCTGGATT CTGGTTCAAG TTTGTGAGGA CCCTGAGACC 
AGTGTGGAAA TTCGCATTTG GGGAGGCATC AAGCAGCTTC TTCATATTTT ACGAGGAGAC 
AGAAATTTTG TTTCCGATCG CTCTTCCATT GGCAGCCTGT CCAGTGCAAA CGCAGCGGGC 
AGAATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCCC GGGAAATACA AGAAAACACT 
TTTTCTCTTC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC TGGTGCTCAA CGACACCAAT 
GCCCACCAGG TGGTCCAGGA AAATGGTGTA TATACAATAG CAAAATTAAT TTTACCAAAC 
AAGCAAAAGA ATGCGGCAAA AACCAACCTA TTACAGTGTT ATGCATTCAG AGCCTTGAGG 
TTTCTCTTCA GTATGGAAAG AAACAGACCA CTCTTTAAAA GACTTTTCCC CACCGACTTG 
TTTGAAATCT TCATTGATAT AGGACATTAT GTTCGTGATA TCAGTGCCTA TGAAGAATTG 
GTATCAAAGT TGAATTTGTT AGTGGAGGAT GAACTGAAGC AAATTGCTGA AAATCTTGAA 
AGCATTAATC AAAATAAAGC TCCTTCGAAA TACATAGGCA ACTATGCAAT TTTGGATCAT 
CTTGGAAGTG GAGCTTTTGG CTGTGTCTAC AAGGTTAGAA AACGCAGTGG TCAAAATCTT 
TTAGCAATGA AAGAGGTTAA TTTACATAAC CCAGCATTTG GAAAGGATAA AAAAGACCGA 
GACAGCAGTG TAAAGAATAT TGTTTCTGAA TTAACCATAA TTAAAGAGCA GCTCTATCAT 
CCCAATGTTG TACGCTATTA CAAAACATTT CTAGAAAATG ATAGGTTGTA TATAGTTATG 
GAGCTTATAG AAGGGGTCCC TCTTGGAGAG CATTTCAGTT CTTTGAAGGA AAAGCAGCAT 
CATTTTATTG AAGAAAGACT ATGGAAAATA TTTATACAGC TGTGCTTAGC TCTTCGGTAC 
TTACACAAGG AGAAGAGGAT TGTTCATAGA GATCTGACAC CAAACAACAT TATGTTGGGG 
GATAAGGACA AAGTAACAGT TACTGACTTT GGGCTGGCAA AGCAAAAACA AGAAAACAGT 
AAACTCACGT CTGTTGTTGG AACAATCCTG TATTCCTGCC CTGAGGTACT GAAGAGTGAA 
CCGTACGGGG AGAAAGCTGA TGTCTGGGCA GCAGGCTGCA TTCTTTATCA GATGGCAACT 
CTGAATCCTC CCTTCTACAG CACCAACATG CTCTCCCTGG CTACAAAAAT AGTGGAGGCA 
GTATATGAAC CAGTGCCAGA AGGCATCTAC TCTGAGAAAG TGACAGATAC CATCAGCAGA 
TGCCTCACTC CTGATGCAGA AACCCGCCCA GATATTATAG AAGTCAGCTC AATGATATCA 
GATGTCATGA TGAAGTATTT AGACAACTTA TCTACATCCC AACTGGCTTT GGAAAAGAAA 
CTGGAACGGG AACGGAAGCG TACACAGAGG TACTTTATGG AAGCCAACCG CAATGCTGTC 
ACATGTCACC ATGAGCTGGC TCTGTTATCT CATGAAACCT TTGAGAAGGC AAGCTTGAGT 
AGCAGCAGCA GTGGAGCTGC CAGCCTGAAA AGCGAACTTT CAGAAAGTGC AGACCTACCC 
CCTGAGGGCT TCCAGGCCCC CTGTGGTAAG GATGAAGACA GGGCCTGTGA TGAAATCCTG 
TCGGAGGAGA ACTTCAACTT GGAAAATATT GAGAAAGATA TGTACTCAGA GCTAGATGAT 
GAACTGGACA TTTCGGACAA CTCTAGCAGC TCCAGTTCAA GCCCTTTGAA AGAATCTACA 
TTCAGCATTT TAAAGAGAAG TTTTAGTGCC TCAGGAGGAG AAAGACAATC CCATACAAGG 
GACTTCACTG GTGGAATTGG ATCCAGACCA AGACCAGCTT TGCTGCCTCT TGACCTGCTT 
CTGAAAGTAC CACCCCTCAT GCTCAGGGCC CACATTAAGG AGCTAGAGGC CGAGTTAGTG 
ACGGGGTGGC AGTCCCATAG CCTTCCCGCT GTGATTCTTC GCAATCTCAA AGATCATGGG 
CCACAGATGA GCACATTCTT GTGGCAAGCA TCTGCAGGGA TTGCTGTGTC CCAGAGGAAA 
GTACGACAGA TCAGTGATCC TATTCAGCAG ATCTTAATTC AGCTGCACAA AATCATCTAT 
ATCACCCAGC TCCCTCCAGC TTTGCACCAC AATTTGAAAA GAAGGGTTAT AGAGAGATTC 
AAGAAATCCC TCTTCAGCCA GCAGAGTAAC CCTTGCAATT TGAAATCTGA AATTAAAAAG 
TTATCTCAGG GATCTCCAGA ACCAATCGAG CCCAACTTTT TCACAACAGA TTACCATTTA 
TTACATCATT CATCAGGTGG AAACAGCCTG TCCCCAGACG AACCAGCAGG TCTGCGTACC 
AGCTTTGATT CAGAGGAGGG AATAACATAT GAACAGATGC AGACTGTGAT TGAAGAAGTC 
CTAGAGGAAA GTGGTTATTA CAACTTTACA TCTAACAGAT ATCACTCCTA TCCATGGGGG 
ACCAAGAATC ACCCAACCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264271.1
CDS	114..3380
Translation	MSSSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_022408563.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408563.1

ORF Insert Sequence:

ATGTCATCAA GTATAAACTA CAAGAATGAG AGAAACTTCA GCAAACATCC CCAGCATAAA 
CTATTTCAGG AGATCTTTAC AGCCTTGGTG AAAAATAGAC TCATCTGCAG AGAGTGGGTT 
CATCGAGCCC CATCTATTCA TTTTCTGAGA GTGTTAATCT GTCTGAGACT TCTAATGAGG 
GATCCATGTT ATCAGGAAAT ACTTCATAGC TTGGGTGGAA TTGAAAACCT AGCTCAGTAC 
ATGGAGATTG TGGCCAATGA GTACCTCAGC TACGGGGAAG AGCAGCATAG TGTGGACAAG 
CTAGTCAACA TGACATATAT TTTTCAAAAA CTTGCTGCTG TCAAAGACCA AAGAGAATGG 
GTCACCACAA GCGGAGCCCA TAAGACATTA GTAAATTTAC TTGGTGCCCG AGATACTAAT 
GTTTTGTTGG GTGCCCTTCT GGCTCTGGCT AGTTTAGCTG AAAGTCCAGA ATGTAGGGAG 
AAGATAAGTG AACTCAACAT TGTAGAAAAT CTGCTGATGA TTTTACATGA ATATGACTTG 
CTTTCCAAAA GATTAACTGC GGAGTTACTA CGCCTACTTT GTGCCGAACC CCAGGTGAAA 
GAGCAGGTGA AGCTCTATGA GGGGATACCC ATCCTCCTCA GTCTGCTCCA CTCTGACCAC 
CTGAAGCTGC TCTGGAGTGT TGTCTGGATT CTGGTTCAAG TTTGTGAGGA CCCTGAGACC 
AGTGTGGAAA TTCGCATTTG GGGAGGCATC AAGCAGCTTC TTCATATTTT ACGAGGAGAC 
AGAAATTTTG TTTCCGATCG CTCTTCCATT GGCAGCCTGT CCAGTGCAAA CGCAGCGGGC 
AGAATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCCC GGGAAATACA AGAAAACACT 
TTTTCTCTTC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC TGGTGCTCAA CGACACCAAT 
GCCCACCAGG TGGTCCAGGA AAATGGTGTA TATACAATAG CAAAATTAAT TTTACCAAAC 
AAGCAAAAGA ATGCGGCAAA AACCAACCTA TTACAGTGTT ATGCATTCAG AGCCTTGAGG 
TTTCTCTTCA GTATGGAAAG AAACAGACCA CTCTTTAAAA GACTTTTCCC CACCGACTTG 
TTTGAAATCT TCATTGATAT AGGACATTAT GTTCGTGATA TCAGTGCCTA TGAAGAATTG 
GTATCAAAGT TGAATTTGTT AGTGGAGGAT GAACTGAAGC AAATTGCTGA AAATCTTGAA 
AGCATTAATC AAAATAAAGC TCCTTCGAAA TACATAGGCA ACTATGCAAT TTTGGATCAT 
CTTGGAAGTG GAGCTTTTGG CTGTGTCTAC AAGGTTAGAA AACGCAGTGG TCAAAATCTT 
TTAGCAATGA AAGAGGTTAA TTTACATAAC CCAGCATTTG GAAAGGATAA AAAAGACCGA 
GACAGCAGTG TAAAGAATAT TGTTTCTGAA TTAACCATAA TTAAAGAGCA GCTCTATCAT 
CCCAATGTTG TACGCTATTA CAAAACATTT CTAGAAAATG ATAGGTTGTA TATAGTTATG 
GAGCTTATAG AAGGGGTCCC TCTTGGAGAG CATTTCAGTT CTTTGAAGGA AAAGCAGCAT 
CATTTTATTG AAGAAAGACT ATGGAAAATA TTTATACAGC TGTGCTTAGC TCTTCGGTAC 
TTACACAAGG AGAAGAGGAT TGTTCATAGA GATCTGACAC CAAACAACAT TATGTTGGGG 
GATAAGGACA AAGTAACAGT TACTGACTTT GGGCTGGCAA AGCAAAAACA AGAAAACAGT 
AAACTCACGT CTGTTGTTGG AACAATCCTG TATTCCTGCC CTGAGGTACT GAAGAGTGAA 
CCGTACGGGG AGAAAGCTGA TGTCTGGGCA GCAGGCTGCA TTCTTTATCA GATGGCAACT 
CTGAATCCTC CCTTCTACAG CACCAACATG CTCTCCCTGG CTACAAAAAT AGTGGAGGCA 
GTATATGAAC CAGTGCCAGA AGGCATCTAC TCTGAGAAAG TGACAGATAC CATCAGCAGA 
TGCCTCACTC CTGATGCAGA AACCCGCCCA GATATTATAG AAGTCAGCTC AATGATATCA 
GATGTCATGA TGAAGTATTT AGACAACTTA TCTACATCCC AACTGGCTTT GGAAAAGAAA 
CTGGAACGGG AACGGAAGCG TACACAGAGG TACTTTATGG AAGCCAACCG CAATGCTGTC 
ACATGTCACC ATGAGCTGGC TCTGTTATCT CATGAAACCT TTGAGAAGGC AAGCTTGAGT 
AGCAGCAGCA GTGGAGCTGC CAGCCTGAAA AGCGAACTTT CAGAAAGTGC AGACCTACCC 
CCTGAGGGCT TCCAGGCCCC CTGTGGTAAG GATGAAGACA GGGCCTGTGA TGAAATCCTG 
TCGGAGGAGA ACTTCAACTT GGAAAATATT GAGAAAGATA TGTACTCAGA GCTAGATGAT 
GAACTGGACA TTTCGGACAA CTCTAGCAGC TCCAGTTCAA GCCCTTTGAA AGAATCTACA 
TTCAGCATTT TAAAGAGAAG TTTTAGTGCC TCAGGAGGAG AAAGACAATC CCATACAAGG 
GACTTCACTG GTGGAATTGG ATCCAGACCA AGACCAGCTT TGCTGCCTCT TGACCTGCTT 
CTGAAAGTAC CACCCCTCAT GCTCAGGGCC CACATTAAGG AGCTAGAGGC CGAGTTAGTG 
ACGGGGTGGC AGTCCCATAG CCTTCCCGCT GTGATTCTTC GCAATCTCAA AGATCATGGG 
CCACAGATGA GCACATTCTT GTGGCAAGCA TCTGCAGGGA TTGCTGTGTC CCAGAGGAAA 
GTACGACAGA TCAGTGATCC TATTCAGCAG ATCTTAATTC AGCTGCACAA AATCATCTAT 
ATCACCCAGC TCCCTCCAGC TTTGCACCAC AATTTGAAAA GAAGGGTTAT AGAGAGATTC 
AAGAAATCCC TCTTCAGCCA GCAGAGTAAC CCTTGCAATT TGAAATCTGA AATTAAAAAG 
TTATCTCAGG GATCTCCAGA ACCAATCGAG CCCAACTTTT TCACAACAGA TTACCATTTA 
TTACATCATT CATCAGGTGG AAACAGCCTG TCCCCAGACG AACCAGCAGG TCTGCGTACC 
AGCTTTGATT CAGAGGAGGG AATAACATAT GAACAGATGC AGACTGTGAT TGAAGAAGTC 
CTAGAGGAAA GTGGTTATTA CAACTTTACA TCTAACAGAT ATCACTCCTA TCCATGGGGG 
ACCAAGAATC ACCCAACCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634341.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634398.1 Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24059
Clone ID Related Accession (Same CDS sequence)	XM_005634341.1 , XM_542757.7 , XM_542757.6 , XM_005634341.2
Accession Version	XM_005634341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634398.1
CDS	118..3636
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634341.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634341.1

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_542757.7 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_542757.3 Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

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CloneID	OCe24059
Clone ID Related Accession (Same CDS sequence)	XM_005634341.1 , XM_542757.7 , XM_542757.6 , XM_005634341.2
Accession Version	XM_542757.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542757.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_542757.3
CDS	144..3662
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_542757.7
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_542757.7

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_542757.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_542757.3 Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe24059
Clone ID Related Accession (Same CDS sequence)	XM_005634341.1 , XM_542757.7 , XM_542757.6 , XM_005634341.2
Accession Version	XM_542757.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542757.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_542757.3
CDS	108..3626
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_542757.6
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_542757.6

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634341.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634398.1 Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X1, mRNA.

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CloneID	OCe24059
Clone ID Related Accession (Same CDS sequence)	XM_005634341.1 , XM_542757.7 , XM_542757.6 , XM_005634341.2
Accession Version	XM_005634341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634398.1
CDS	192..3710
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634341.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634341.2

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GCTCCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGCAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCAATCG AGCCCAACTT TTTCACAACA 
GATTACCATT TATTACATCA TTCATCAGGT GGAAACAGCC TGTCCCCAGA CGAACCAGCA 
GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT TACAACTTTA CATCTAACAG ATATCACTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634342.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634399.1 Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X3, mRNA.

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CloneID	OCe24060
Clone ID Related Accession (Same CDS sequence)	XM_005634342.1 , XM_005634342.2
Accession Version	XM_005634342.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG CATCTGCAGG GATTGCTGTG TCCCAGAGGA AAGTACGACA GATCAGTGAT 
CCTATTCAGC AGATCTTAAT TCAGCTGCAC AAAATCATCT ATATCACCCA GCTCCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGCAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCAATCG AGCCCAACTT TTTCACAACA GATTACCATT TATTACATCA TTCATCAGGT 
GGAAACAGCC TGTCCCCAGA CGAACCAGCA GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT 
TACAACTTTA CATCTAACAG ATATCACTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634399.1
CDS	118..3606
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634342.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634342.1

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG CATCTGCAGG GATTGCTGTG TCCCAGAGGA AAGTACGACA GATCAGTGAT 
CCTATTCAGC AGATCTTAAT TCAGCTGCAC AAAATCATCT ATATCACCCA GCTCCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGCAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCAATCG AGCCCAACTT TTTCACAACA GATTACCATT TATTACATCA TTCATCAGGT 
GGAAACAGCC TGTCCCCAGA CGAACCAGCA GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT 
TACAACTTTA CATCTAACAG ATATCACTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634342.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634399.1 Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe24060
Clone ID Related Accession (Same CDS sequence)	XM_005634342.1 , XM_005634342.2
Accession Version	XM_005634342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG CATCTGCAGG GATTGCTGTG TCCCAGAGGA AAGTACGACA GATCAGTGAT 
CCTATTCAGC AGATCTTAAT TCAGCTGCAC AAAATCATCT ATATCACCCA GCTCCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGCAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCAATCG AGCCCAACTT TTTCACAACA GATTACCATT TATTACATCA TTCATCAGGT 
GGAAACAGCC TGTCCCCAGA CGAACCAGCA GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT 
TACAACTTTA CATCTAACAG ATATCACTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634399.1
CDS	192..3680
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634342.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634342.2

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG CATCTGCAGG GATTGCTGTG TCCCAGAGGA AAGTACGACA GATCAGTGAT 
CCTATTCAGC AGATCTTAAT TCAGCTGCAC AAAATCATCT ATATCACCCA GCTCCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGCAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCAATCG AGCCCAACTT TTTCACAACA GATTACCATT TATTACATCA TTCATCAGGT 
GGAAACAGCC TGTCCCCAGA CGAACCAGCA GGTCTGCGTA CCAGCTTTGA TTCAGAGGAG 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTAGAGGA AAGTGGTTAT 
TACAACTTTA CATCTAACAG ATATCACTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634343.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634400.1 Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X4, mRNA.

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CloneID	OCe24061
Clone ID Related Accession (Same CDS sequence)	XM_005634343.1
Accession Version	XM_005634343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek10 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GTTATCTCAG GGATCTCCAG AACCAATCGA GCCCAACTTT 
TTCACAACAG ATTACCATTT ATTACATCAT TCATCAGGTG GAAACAGCCT GTCCCCAGAC 
GAACCAGCAG GTCTGCGTAC CAGCTTTGAT TCAGAGGAGG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGTTATT ACAACTTTAC ATCTAACAGA 
TATCACTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634400.1
CDS	118..3525
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634343.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634343.1

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT ACCACCCCTC ATGCTCAGGG CCCACATTAA GGAGCTAGAG 
GCCGAGTTAG TGACGGGGTG GCAGTCCCAT AGCCTTCCCG CTGTGATTCT TCGCAATCTC 
AAAGATCATG GGCCACAGAT GAGCACATTC TTGTGGCAAG CATCTGCAGG GATTGCTGTG 
TCCCAGAGGA AAGTACGACA GATCAGTGAT CCTATTCAGC AGATCTTAAT TCAGCTGCAC 
AAAATCATCT ATATCACCCA GTTATCTCAG GGATCTCCAG AACCAATCGA GCCCAACTTT 
TTCACAACAG ATTACCATTT ATTACATCAT TCATCAGGTG GAAACAGCCT GTCCCCAGAC 
GAACCAGCAG GTCTGCGTAC CAGCTTTGAT TCAGAGGAGG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGTTATT ACAACTTTAC ATCTAACAGA 
TATCACTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634344.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634401.1 Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.

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CloneID	OCe24062
Clone ID Related Accession (Same CDS sequence)	XM_005634344.2 , XM_005634344.1
Accession Version	XM_005634344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC ATCTGCAGGG 
ATTGCTGTGT CCCAGAGGAA AGTACGACAG ATCAGTGATC CTATTCAGCA GATCTTAATT 
CAGCTGCACA AAATCATCTA TATCACCCAG CTCCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGCAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCAATCGA GCCCAACTTT 
TTCACAACAG ATTACCATTT ATTACATCAT TCATCAGGTG GAAACAGCCT GTCCCCAGAC 
GAACCAGCAG GTCTGCGTAC CAGCTTTGAT TCAGAGGAGG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGTTATT ACAACTTTAC ATCTAACAGA 
TATCACTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634401.1
CDS	192..3539
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634344.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634344.2

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC ATCTGCAGGG 
ATTGCTGTGT CCCAGAGGAA AGTACGACAG ATCAGTGATC CTATTCAGCA GATCTTAATT 
CAGCTGCACA AAATCATCTA TATCACCCAG CTCCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGCAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCAATCGA GCCCAACTTT 
TTCACAACAG ATTACCATTT ATTACATCAT TCATCAGGTG GAAACAGCCT GTCCCCAGAC 
GAACCAGCAG GTCTGCGTAC CAGCTTTGAT TCAGAGGAGG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGTTATT ACAACTTTAC ATCTAACAGA 
TATCACTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634344.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634401.1 Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe24062
Clone ID Related Accession (Same CDS sequence)	XM_005634344.2 , XM_005634344.1
Accession Version	XM_005634344.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek10 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC ATCTGCAGGG 
ATTGCTGTGT CCCAGAGGAA AGTACGACAG ATCAGTGATC CTATTCAGCA GATCTTAATT 
CAGCTGCACA AAATCATCTA TATCACCCAG CTCCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGCAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCAATCGA GCCCAACTTT 
TTCACAACAG ATTACCATTT ATTACATCAT TCATCAGGTG GAAACAGCCT GTCCCCAGAC 
GAACCAGCAG GTCTGCGTAC CAGCTTTGAT TCAGAGGAGG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGTTATT ACAACTTTAC ATCTAACAGA 
TATCACTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634401.1
CDS	118..3465
Translation	MPDQDKKVKTTEKSTDKKQGITTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEHVIKVGGHRTRGQWHESTEAVELENFSINYKNERNFSKHPQHKLFQEIFTALVKNRLICREWVHRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634344.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634344.1

ORF Insert Sequence:

ATGCCTGATC AAGACAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA AAAACAAGGA 
ATCACCACCA GGGACTACTC AGATCTTAAA AGACTTCGTT GCCTTTTGAA CGTCCAGTCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCC 
GAGCATGTCA TCAAGGTGGG TGGACATAGA ACCCGAGGTC AGTGGCATGA GTCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAACTT CAGCAAACAT 
CCCCAGCATA AACTATTTCA GGAGATCTTT ACAGCCTTGG TGAAAAATAG ACTCATCTGC 
AGAGAGTGGG TTCATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGA 
CTTCTAATGA GGGATCCATG TTATCAGGAA ATACTTCATA GCTTGGGTGG AATTGAAAAC 
CTAGCTCAGT ACATGGAGAT TGTGGCCAAT GAGTACCTCA GCTACGGGGA AGAGCAGCAT 
AGTGTGGACA AGCTAGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC TGTCAAAGAC 
CAAAGAGAAT GGGTCACCAC AAGCGGAGCC CATAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTTTGTT GGGTGCCCTT CTGGCTCTGG CTAGTTTAGC TGAAAGTCCA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGCTGAT GATTTTACAT 
GAATATGACT TGCTTTCCAA AAGATTAACT GCGGAGTTAC TACGCCTACT TTGTGCCGAA 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CCATCCTCCT CAGTCTGCTC 
CACTCTGACC ACCTGAAGCT GCTCTGGAGT GTTGTCTGGA TTCTGGTTCA AGTTTGTGAG 
GACCCTGAGA CCAGTGTGGA AATTCGCATT TGGGGAGGCA TCAAGCAGCT TCTTCATATT 
TTACGAGGAG ACAGAAATTT TGTTTCCGAT CGCTCTTCCA TTGGCAGCCT GTCCAGTGCA 
AACGCAGCGG GCAGAATCCA GCAGCTTCAT TTGTCAGAAG ATTTAAGCCC CCGGGAAATA 
CAAGAAAACA CTTTTTCTCT TCAAGCAGCC TGCTGTGCTG CCCTCACCGA GCTGGTGCTC 
AACGACACCA ATGCCCACCA GGTGGTCCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ACAAGCAAAA GAATGCGGCA AAAACCAACC TATTACAGTG TTATGCATTC 
AGAGCCTTGA GGTTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACCGACT TGTTTGAAAT CTTCATTGAT ATAGGACATT ATGTTCGTGA TATCAGTGCC 
TATGAAGAAT TGGTATCAAA GTTGAATTTG TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATCTTG AAAGCATTAA TCAAAATAAA GCTCCTTCGA AATACATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTCT ACAAGGTTAG AAAACGCAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTT AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAAAAAGACC GAGACAGCAG TGTAAAGAAT ATTGTTTCTG AATTAACCAT AATTAAAGAG 
CAGCTCTATC ATCCCAATGT TGTACGCTAT TACAAAACAT TTCTAGAAAA TGATAGGTTG 
TATATAGTTA TGGAGCTTAT AGAAGGGGTC CCTCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAGCAGC ATCATTTTAT TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGGT ACTTACACAA GGAGAAGAGG ATTGTTCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACA GTTACTGACT TTGGGCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC GTCTGTTGTT GGAACAATCC TGTATTCCTG CCCTGAGGTA 
CTGAAGAGTG AACCGTACGG GGAGAAAGCT GATGTCTGGG CAGCAGGCTG CATTCTTTAT 
CAGATGGCAA CTCTGAATCC TCCCTTCTAC AGCACCAACA TGCTCTCCCT GGCTACAAAA 
ATAGTGGAGG CAGTATATGA ACCAGTGCCA GAAGGCATCT ACTCTGAGAA AGTGACAGAT 
ACCATCAGCA GATGCCTCAC TCCTGATGCA GAAACCCGCC CAGATATTAT AGAAGTCAGC 
TCAATGATAT CAGATGTCAT GATGAAGTAT TTAGACAACT TATCTACATC CCAACTGGCT 
TTGGAAAAGA AACTGGAACG GGAACGGAAG CGTACACAGA GGTACTTTAT GGAAGCCAAC 
CGCAATGCTG TCACATGTCA CCATGAGCTG GCTCTGTTAT CTCATGAAAC CTTTGAGAAG 
GCAAGCTTGA GTAGCAGCAG CAGTGGAGCT GCCAGCCTGA AAAGCGAACT TTCAGAAAGT 
GCAGACCTAC CCCCTGAGGG CTTCCAGGCC CCCTGTGGTA AGGATGAAGA CAGGGCCTGT 
GATGAAATCC TGTCGGAGGA GAACTTCAAC TTGGAAAATA TTGAGAAAGA TATGTACTCA 
GAGCTAGATG ATGAACTGGA CATTTCGGAC AACTCTAGCA GCTCCAGTTC AAGCCCTTTG 
AAAGAATCTA CATTCAGCAT TTTAAAGAGA AGTTTTAGTG CCTCAGGAGG AGAAAGACAA 
TCCCATACAA GGGACTTCAC TGGTGGAATT GGATCCAGAC CAAGACCAGC ATCTGCAGGG 
ATTGCTGTGT CCCAGAGGAA AGTACGACAG ATCAGTGATC CTATTCAGCA GATCTTAATT 
CAGCTGCACA AAATCATCTA TATCACCCAG CTCCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGCAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCAATCGA GCCCAACTTT 
TTCACAACAG ATTACCATTT ATTACATCAT TCATCAGGTG GAAACAGCCT GTCCCCAGAC 
GAACCAGCAG GTCTGCGTAC CAGCTTTGAT TCAGAGGAGG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTAGAGGAA AGTGGTTATT ACAACTTTAC ATCTAACAGA 
TATCACTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634345.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634402.1 Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe24063
Clone ID Related Accession (Same CDS sequence)	XM_005634345.1 , XM_005634345.2
Accession Version	XM_005634345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek10 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATTG TGGCCAATGA GTACCTCAGC TACGGGGAAG AGCAGCATAG TGTGGACAAG 
CTAGTCAACA TGACATATAT TTTTCAAAAA CTTGCTGCTG TCAAAGACCA AAGAGAATGG 
GTCACCACAA GCGGAGCCCA TAAGACATTA GTAAATTTAC TTGGTGCCCG AGATACTAAT 
GTTTTGTTGG GTGCCCTTCT GGCTCTGGCT AGTTTAGCTG AAAGTCCAGA ATGTAGGGAG 
AAGATAAGTG AACTCAACAT TGTAGAAAAT CTGCTGATGA TTTTACATGA ATATGACTTG 
CTTTCCAAAA GATTAACTGC GGAGTTACTA CGCCTACTTT GTGCCGAACC CCAGGTGAAA 
GAGCAGGTGA AGCTCTATGA GGGGATACCC ATCCTCCTCA GTCTGCTCCA CTCTGACCAC 
CTGAAGCTGC TCTGGAGTGT TGTCTGGATT CTGGTTCAAG TTTGTGAGGA CCCTGAGACC 
AGTGTGGAAA TTCGCATTTG GGGAGGCATC AAGCAGCTTC TTCATATTTT ACGAGGAGAC 
AGAAATTTTG TTTCCGATCG CTCTTCCATT GGCAGCCTGT CCAGTGCAAA CGCAGCGGGC 
AGAATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCCC GGGAAATACA AGAAAACACT 
TTTTCTCTTC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC TGGTGCTCAA CGACACCAAT 
GCCCACCAGG TGGTCCAGGA AAATGGTGTA TATACAATAG CAAAATTAAT TTTACCAAAC 
AAGCAAAAGA ATGCGGCAAA AACCAACCTA TTACAGTGTT ATGCATTCAG AGCCTTGAGG 
TTTCTCTTCA GTATGGAAAG AAACAGACCA CTCTTTAAAA GACTTTTCCC CACCGACTTG 
TTTGAAATCT TCATTGATAT AGGACATTAT GTTCGTGATA TCAGTGCCTA TGAAGAATTG 
GTATCAAAGT TGAATTTGTT AGTGGAGGAT GAACTGAAGC AAATTGCTGA AAATCTTGAA 
AGCATTAATC AAAATAAAGC TCCTTCGAAA TACATAGGCA ACTATGCAAT TTTGGATCAT 
CTTGGAAGTG GAGCTTTTGG CTGTGTCTAC AAGGTTAGAA AACGCAGTGG TCAAAATCTT 
TTAGCAATGA AAGAGGTTAA TTTACATAAC CCAGCATTTG GAAAGGATAA AAAAGACCGA 
GACAGCAGTG TAAAGAATAT TGTTTCTGAA TTAACCATAA TTAAAGAGCA GCTCTATCAT 
CCCAATGTTG TACGCTATTA CAAAACATTT CTAGAAAATG ATAGGTTGTA TATAGTTATG 
GAGCTTATAG AAGGGGTCCC TCTTGGAGAG CATTTCAGTT CTTTGAAGGA AAAGCAGCAT 
CATTTTATTG AAGAAAGACT ATGGAAAATA TTTATACAGC TGTGCTTAGC TCTTCGGTAC 
TTACACAAGG AGAAGAGGAT TGTTCATAGA GATCTGACAC CAAACAACAT TATGTTGGGG 
GATAAGGACA AAGTAACAGT TACTGACTTT GGGCTGGCAA AGCAAAAACA AGAAAACAGT 
AAACTCACGT CTGTTGTTGG AACAATCCTG TATTCCTGCC CTGAGGTACT GAAGAGTGAA 
CCGTACGGGG AGAAAGCTGA TGTCTGGGCA GCAGGCTGCA TTCTTTATCA GATGGCAACT 
CTGAATCCTC CCTTCTACAG CACCAACATG CTCTCCCTGG CTACAAAAAT AGTGGAGGCA 
GTATATGAAC CAGTGCCAGA AGGCATCTAC TCTGAGAAAG TGACAGATAC CATCAGCAGA 
TGCCTCACTC CTGATGCAGA AACCCGCCCA GATATTATAG AAGTCAGCTC AATGATATCA 
GATGTCATGA TGAAGTATTT AGACAACTTA TCTACATCCC AACTGGCTTT GGAAAAGAAA 
CTGGAACGGG AACGGAAGCG TACACAGAGG TACTTTATGG AAGCCAACCG CAATGCTGTC 
ACATGTCACC ATGAGCTGGC TCTGTTATCT CATGAAACCT TTGAGAAGGC AAGCTTGAGT 
AGCAGCAGCA GTGGAGCTGC CAGCCTGAAA AGCGAACTTT CAGAAAGTGC AGACCTACCC 
CCTGAGGGCT TCCAGGCCCC CTGTGGTAAG GATGAAGACA GGGCCTGTGA TGAAATCCTG 
TCGGAGGAGA ACTTCAACTT GGAAAATATT GAGAAAGATA TGTACTCAGA GCTAGATGAT 
GAACTGGACA TTTCGGACAA CTCTAGCAGC TCCAGTTCAA GCCCTTTGAA AGAATCTACA 
TTCAGCATTT TAAAGAGAAG TTTTAGTGCC TCAGGAGGAG AAAGACAATC CCATACAAGG 
GACTTCACTG GTGGAATTGG ATCCAGACCA AGACCAGCTT TGCTGCCTCT TGACCTGCTT 
CTGAAAGTAC CACCCCTCAT GCTCAGGGCC CACATTAAGG AGCTAGAGGC CGAGTTAGTG 
ACGGGGTGGC AGTCCCATAG CCTTCCCGCT GTGATTCTTC GCAATCTCAA AGATCATGGG 
CCACAGATGA GCACATTCTT GTGGCAAGCA TCTGCAGGGA TTGCTGTGTC CCAGAGGAAA 
GTACGACAGA TCAGTGATCC TATTCAGCAG ATCTTAATTC AGCTGCACAA AATCATCTAT 
ATCACCCAGC TCCCTCCAGC TTTGCACCAC AATTTGAAAA GAAGGGTTAT AGAGAGATTC 
AAGAAATCCC TCTTCAGCCA GCAGAGTAAC CCTTGCAATT TGAAATCTGA AATTAAAAAG 
TTATCTCAGG GATCTCCAGA ACCAATCGAG CCCAACTTTT TCACAACAGA TTACCATTTA 
TTACATCATT CATCAGGTGG AAACAGCCTG TCCCCAGACG AACCAGCAGG TCTGCGTACC 
AGCTTTGATT CAGAGGAGGG AATAACATAT GAACAGATGC AGACTGTGAT TGAAGAAGTC 
CTAGAGGAAA GTGGTTATTA CAACTTTACA TCTAACAGAT ATCACTCCTA TCCATGGGGG 
ACCAAGAATC ACCCAACCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634402.1
CDS	125..3151
Translation	MEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634345.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 10 (NEK10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634345.1

ORF Insert Sequence:

ATGGAGATTG TGGCCAATGA GTACCTCAGC TACGGGGAAG AGCAGCATAG TGTGGACAAG 
CTAGTCAACA TGACATATAT TTTTCAAAAA CTTGCTGCTG TCAAAGACCA AAGAGAATGG 
GTCACCACAA GCGGAGCCCA TAAGACATTA GTAAATTTAC TTGGTGCCCG AGATACTAAT 
GTTTTGTTGG GTGCCCTTCT GGCTCTGGCT AGTTTAGCTG AAAGTCCAGA ATGTAGGGAG 
AAGATAAGTG AACTCAACAT TGTAGAAAAT CTGCTGATGA TTTTACATGA ATATGACTTG 
CTTTCCAAAA GATTAACTGC GGAGTTACTA CGCCTACTTT GTGCCGAACC CCAGGTGAAA 
GAGCAGGTGA AGCTCTATGA GGGGATACCC ATCCTCCTCA GTCTGCTCCA CTCTGACCAC 
CTGAAGCTGC TCTGGAGTGT TGTCTGGATT CTGGTTCAAG TTTGTGAGGA CCCTGAGACC 
AGTGTGGAAA TTCGCATTTG GGGAGGCATC AAGCAGCTTC TTCATATTTT ACGAGGAGAC 
AGAAATTTTG TTTCCGATCG CTCTTCCATT GGCAGCCTGT CCAGTGCAAA CGCAGCGGGC 
AGAATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCCC GGGAAATACA AGAAAACACT 
TTTTCTCTTC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC TGGTGCTCAA CGACACCAAT 
GCCCACCAGG TGGTCCAGGA AAATGGTGTA TATACAATAG CAAAATTAAT TTTACCAAAC 
AAGCAAAAGA ATGCGGCAAA AACCAACCTA TTACAGTGTT ATGCATTCAG AGCCTTGAGG 
TTTCTCTTCA GTATGGAAAG AAACAGACCA CTCTTTAAAA GACTTTTCCC CACCGACTTG 
TTTGAAATCT TCATTGATAT AGGACATTAT GTTCGTGATA TCAGTGCCTA TGAAGAATTG 
GTATCAAAGT TGAATTTGTT AGTGGAGGAT GAACTGAAGC AAATTGCTGA AAATCTTGAA 
AGCATTAATC AAAATAAAGC TCCTTCGAAA TACATAGGCA ACTATGCAAT TTTGGATCAT 
CTTGGAAGTG GAGCTTTTGG CTGTGTCTAC AAGGTTAGAA AACGCAGTGG TCAAAATCTT 
TTAGCAATGA AAGAGGTTAA TTTACATAAC CCAGCATTTG GAAAGGATAA AAAAGACCGA 
GACAGCAGTG TAAAGAATAT TGTTTCTGAA TTAACCATAA TTAAAGAGCA GCTCTATCAT 
CCCAATGTTG TACGCTATTA CAAAACATTT CTAGAAAATG ATAGGTTGTA TATAGTTATG 
GAGCTTATAG AAGGGGTCCC TCTTGGAGAG CATTTCAGTT CTTTGAAGGA AAAGCAGCAT 
CATTTTATTG AAGAAAGACT ATGGAAAATA TTTATACAGC TGTGCTTAGC TCTTCGGTAC 
TTACACAAGG AGAAGAGGAT TGTTCATAGA GATCTGACAC CAAACAACAT TATGTTGGGG 
GATAAGGACA AAGTAACAGT TACTGACTTT GGGCTGGCAA AGCAAAAACA AGAAAACAGT 
AAACTCACGT CTGTTGTTGG AACAATCCTG TATTCCTGCC CTGAGGTACT GAAGAGTGAA 
CCGTACGGGG AGAAAGCTGA TGTCTGGGCA GCAGGCTGCA TTCTTTATCA GATGGCAACT 
CTGAATCCTC CCTTCTACAG CACCAACATG CTCTCCCTGG CTACAAAAAT AGTGGAGGCA 
GTATATGAAC CAGTGCCAGA AGGCATCTAC TCTGAGAAAG TGACAGATAC CATCAGCAGA 
TGCCTCACTC CTGATGCAGA AACCCGCCCA GATATTATAG AAGTCAGCTC AATGATATCA 
GATGTCATGA TGAAGTATTT AGACAACTTA TCTACATCCC AACTGGCTTT GGAAAAGAAA 
CTGGAACGGG AACGGAAGCG TACACAGAGG TACTTTATGG AAGCCAACCG CAATGCTGTC 
ACATGTCACC ATGAGCTGGC TCTGTTATCT CATGAAACCT TTGAGAAGGC AAGCTTGAGT 
AGCAGCAGCA GTGGAGCTGC CAGCCTGAAA AGCGAACTTT CAGAAAGTGC AGACCTACCC 
CCTGAGGGCT TCCAGGCCCC CTGTGGTAAG GATGAAGACA GGGCCTGTGA TGAAATCCTG 
TCGGAGGAGA ACTTCAACTT GGAAAATATT GAGAAAGATA TGTACTCAGA GCTAGATGAT 
GAACTGGACA TTTCGGACAA CTCTAGCAGC TCCAGTTCAA GCCCTTTGAA AGAATCTACA 
TTCAGCATTT TAAAGAGAAG TTTTAGTGCC TCAGGAGGAG AAAGACAATC CCATACAAGG 
GACTTCACTG GTGGAATTGG ATCCAGACCA AGACCAGCTT TGCTGCCTCT TGACCTGCTT 
CTGAAAGTAC CACCCCTCAT GCTCAGGGCC CACATTAAGG AGCTAGAGGC CGAGTTAGTG 
ACGGGGTGGC AGTCCCATAG CCTTCCCGCT GTGATTCTTC GCAATCTCAA AGATCATGGG 
CCACAGATGA GCACATTCTT GTGGCAAGCA TCTGCAGGGA TTGCTGTGTC CCAGAGGAAA 
GTACGACAGA TCAGTGATCC TATTCAGCAG ATCTTAATTC AGCTGCACAA AATCATCTAT 
ATCACCCAGC TCCCTCCAGC TTTGCACCAC AATTTGAAAA GAAGGGTTAT AGAGAGATTC 
AAGAAATCCC TCTTCAGCCA GCAGAGTAAC CCTTGCAATT TGAAATCTGA AATTAAAAAG 
TTATCTCAGG GATCTCCAGA ACCAATCGAG CCCAACTTTT TCACAACAGA TTACCATTTA 
TTACATCATT CATCAGGTGG AAACAGCCTG TCCCCAGACG AACCAGCAGG TCTGCGTACC 
AGCTTTGATT CAGAGGAGGG AATAACATAT GAACAGATGC AGACTGTGAT TGAAGAAGTC 
CTAGAGGAAA GTGGTTATTA CAACTTTACA TCTAACAGAT ATCACTCCTA TCCATGGGGG 
ACCAAGAATC ACCCAACCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_005634345.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005634402.1 Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe24063
Clone ID Related Accession (Same CDS sequence)	XM_005634345.1 , XM_005634345.2
Accession Version	XM_005634345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATTG TGGCCAATGA GTACCTCAGC TACGGGGAAG AGCAGCATAG TGTGGACAAG 
CTAGTCAACA TGACATATAT TTTTCAAAAA CTTGCTGCTG TCAAAGACCA AAGAGAATGG 
GTCACCACAA GCGGAGCCCA TAAGACATTA GTAAATTTAC TTGGTGCCCG AGATACTAAT 
GTTTTGTTGG GTGCCCTTCT GGCTCTGGCT AGTTTAGCTG AAAGTCCAGA ATGTAGGGAG 
AAGATAAGTG AACTCAACAT TGTAGAAAAT CTGCTGATGA TTTTACATGA ATATGACTTG 
CTTTCCAAAA GATTAACTGC GGAGTTACTA CGCCTACTTT GTGCCGAACC CCAGGTGAAA 
GAGCAGGTGA AGCTCTATGA GGGGATACCC ATCCTCCTCA GTCTGCTCCA CTCTGACCAC 
CTGAAGCTGC TCTGGAGTGT TGTCTGGATT CTGGTTCAAG TTTGTGAGGA CCCTGAGACC 
AGTGTGGAAA TTCGCATTTG GGGAGGCATC AAGCAGCTTC TTCATATTTT ACGAGGAGAC 
AGAAATTTTG TTTCCGATCG CTCTTCCATT GGCAGCCTGT CCAGTGCAAA CGCAGCGGGC 
AGAATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCCC GGGAAATACA AGAAAACACT 
TTTTCTCTTC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC TGGTGCTCAA CGACACCAAT 
GCCCACCAGG TGGTCCAGGA AAATGGTGTA TATACAATAG CAAAATTAAT TTTACCAAAC 
AAGCAAAAGA ATGCGGCAAA AACCAACCTA TTACAGTGTT ATGCATTCAG AGCCTTGAGG 
TTTCTCTTCA GTATGGAAAG AAACAGACCA CTCTTTAAAA GACTTTTCCC CACCGACTTG 
TTTGAAATCT TCATTGATAT AGGACATTAT GTTCGTGATA TCAGTGCCTA TGAAGAATTG 
GTATCAAAGT TGAATTTGTT AGTGGAGGAT GAACTGAAGC AAATTGCTGA AAATCTTGAA 
AGCATTAATC AAAATAAAGC TCCTTCGAAA TACATAGGCA ACTATGCAAT TTTGGATCAT 
CTTGGAAGTG GAGCTTTTGG CTGTGTCTAC AAGGTTAGAA AACGCAGTGG TCAAAATCTT 
TTAGCAATGA AAGAGGTTAA TTTACATAAC CCAGCATTTG GAAAGGATAA AAAAGACCGA 
GACAGCAGTG TAAAGAATAT TGTTTCTGAA TTAACCATAA TTAAAGAGCA GCTCTATCAT 
CCCAATGTTG TACGCTATTA CAAAACATTT CTAGAAAATG ATAGGTTGTA TATAGTTATG 
GAGCTTATAG AAGGGGTCCC TCTTGGAGAG CATTTCAGTT CTTTGAAGGA AAAGCAGCAT 
CATTTTATTG AAGAAAGACT ATGGAAAATA TTTATACAGC TGTGCTTAGC TCTTCGGTAC 
TTACACAAGG AGAAGAGGAT TGTTCATAGA GATCTGACAC CAAACAACAT TATGTTGGGG 
GATAAGGACA AAGTAACAGT TACTGACTTT GGGCTGGCAA AGCAAAAACA AGAAAACAGT 
AAACTCACGT CTGTTGTTGG AACAATCCTG TATTCCTGCC CTGAGGTACT GAAGAGTGAA 
CCGTACGGGG AGAAAGCTGA TGTCTGGGCA GCAGGCTGCA TTCTTTATCA GATGGCAACT 
CTGAATCCTC CCTTCTACAG CACCAACATG CTCTCCCTGG CTACAAAAAT AGTGGAGGCA 
GTATATGAAC CAGTGCCAGA AGGCATCTAC TCTGAGAAAG TGACAGATAC CATCAGCAGA 
TGCCTCACTC CTGATGCAGA AACCCGCCCA GATATTATAG AAGTCAGCTC AATGATATCA 
GATGTCATGA TGAAGTATTT AGACAACTTA TCTACATCCC AACTGGCTTT GGAAAAGAAA 
CTGGAACGGG AACGGAAGCG TACACAGAGG TACTTTATGG AAGCCAACCG CAATGCTGTC 
ACATGTCACC ATGAGCTGGC TCTGTTATCT CATGAAACCT TTGAGAAGGC AAGCTTGAGT 
AGCAGCAGCA GTGGAGCTGC CAGCCTGAAA AGCGAACTTT CAGAAAGTGC AGACCTACCC 
CCTGAGGGCT TCCAGGCCCC CTGTGGTAAG GATGAAGACA GGGCCTGTGA TGAAATCCTG 
TCGGAGGAGA ACTTCAACTT GGAAAATATT GAGAAAGATA TGTACTCAGA GCTAGATGAT 
GAACTGGACA TTTCGGACAA CTCTAGCAGC TCCAGTTCAA GCCCTTTGAA AGAATCTACA 
TTCAGCATTT TAAAGAGAAG TTTTAGTGCC TCAGGAGGAG AAAGACAATC CCATACAAGG 
GACTTCACTG GTGGAATTGG ATCCAGACCA AGACCAGCTT TGCTGCCTCT TGACCTGCTT 
CTGAAAGTAC CACCCCTCAT GCTCAGGGCC CACATTAAGG AGCTAGAGGC CGAGTTAGTG 
ACGGGGTGGC AGTCCCATAG CCTTCCCGCT GTGATTCTTC GCAATCTCAA AGATCATGGG 
CCACAGATGA GCACATTCTT GTGGCAAGCA TCTGCAGGGA TTGCTGTGTC CCAGAGGAAA 
GTACGACAGA TCAGTGATCC TATTCAGCAG ATCTTAATTC AGCTGCACAA AATCATCTAT 
ATCACCCAGC TCCCTCCAGC TTTGCACCAC AATTTGAAAA GAAGGGTTAT AGAGAGATTC 
AAGAAATCCC TCTTCAGCCA GCAGAGTAAC CCTTGCAATT TGAAATCTGA AATTAAAAAG 
TTATCTCAGG GATCTCCAGA ACCAATCGAG CCCAACTTTT TCACAACAGA TTACCATTTA 
TTACATCATT CATCAGGTGG AAACAGCCTG TCCCCAGACG AACCAGCAGG TCTGCGTACC 
AGCTTTGATT CAGAGGAGGG AATAACATAT GAACAGATGC AGACTGTGAT TGAAGAAGTC 
CTAGAGGAAA GTGGTTATTA CAACTTTACA TCTAACAGAT ATCACTCCTA TCCATGGGGG 
ACCAAGAATC ACCCAACCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005634402.1
CDS	145..3171
Translation	MEIVANEYLSYGEEQHSVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGALLALASLAESPECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPILLSLLHSDHLKLLWSVVWILVQVCEDPETSVEIRIWGGIKQLLHILRGDRNFVSDRSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKTNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENLESINQNKAPSKYIGNYAILDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKDKKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGVPLGEHFSSLKEKQHHFIEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAAGCILYQMATLNPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAETRPDIIEVSSMISDVMMKYLDNLSTSQLALEKKLERERKRTQRYFMEANRNAVTCHHELALLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQAPCGKDEDRACDEILSEENFNLENIEKDMYSELDDELDISDNSSSSSSSPLKESTFSILKRSFSASGGERQSHTRDFTGGIGSRPRPALLPLDLLLKVPPLMLRAHIKELEAELVTGWQSHSLPAVILRNLKDHGPQMSTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTTDYHLLHHSSGGNSLSPDEPAGLRTSFDSEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_005634345.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634345.2

ORF Insert Sequence:

ATGGAGATTG TGGCCAATGA GTACCTCAGC TACGGGGAAG AGCAGCATAG TGTGGACAAG 
CTAGTCAACA TGACATATAT TTTTCAAAAA CTTGCTGCTG TCAAAGACCA AAGAGAATGG 
GTCACCACAA GCGGAGCCCA TAAGACATTA GTAAATTTAC TTGGTGCCCG AGATACTAAT 
GTTTTGTTGG GTGCCCTTCT GGCTCTGGCT AGTTTAGCTG AAAGTCCAGA ATGTAGGGAG 
AAGATAAGTG AACTCAACAT TGTAGAAAAT CTGCTGATGA TTTTACATGA ATATGACTTG 
CTTTCCAAAA GATTAACTGC GGAGTTACTA CGCCTACTTT GTGCCGAACC CCAGGTGAAA 
GAGCAGGTGA AGCTCTATGA GGGGATACCC ATCCTCCTCA GTCTGCTCCA CTCTGACCAC 
CTGAAGCTGC TCTGGAGTGT TGTCTGGATT CTGGTTCAAG TTTGTGAGGA CCCTGAGACC 
AGTGTGGAAA TTCGCATTTG GGGAGGCATC AAGCAGCTTC TTCATATTTT ACGAGGAGAC 
AGAAATTTTG TTTCCGATCG CTCTTCCATT GGCAGCCTGT CCAGTGCAAA CGCAGCGGGC 
AGAATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCCC GGGAAATACA AGAAAACACT 
TTTTCTCTTC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC TGGTGCTCAA CGACACCAAT 
GCCCACCAGG TGGTCCAGGA AAATGGTGTA TATACAATAG CAAAATTAAT TTTACCAAAC 
AAGCAAAAGA ATGCGGCAAA AACCAACCTA TTACAGTGTT ATGCATTCAG AGCCTTGAGG 
TTTCTCTTCA GTATGGAAAG AAACAGACCA CTCTTTAAAA GACTTTTCCC CACCGACTTG 
TTTGAAATCT TCATTGATAT AGGACATTAT GTTCGTGATA TCAGTGCCTA TGAAGAATTG 
GTATCAAAGT TGAATTTGTT AGTGGAGGAT GAACTGAAGC AAATTGCTGA AAATCTTGAA 
AGCATTAATC AAAATAAAGC TCCTTCGAAA TACATAGGCA ACTATGCAAT TTTGGATCAT 
CTTGGAAGTG GAGCTTTTGG CTGTGTCTAC AAGGTTAGAA AACGCAGTGG TCAAAATCTT 
TTAGCAATGA AAGAGGTTAA TTTACATAAC CCAGCATTTG GAAAGGATAA AAAAGACCGA 
GACAGCAGTG TAAAGAATAT TGTTTCTGAA TTAACCATAA TTAAAGAGCA GCTCTATCAT 
CCCAATGTTG TACGCTATTA CAAAACATTT CTAGAAAATG ATAGGTTGTA TATAGTTATG 
GAGCTTATAG AAGGGGTCCC TCTTGGAGAG CATTTCAGTT CTTTGAAGGA AAAGCAGCAT 
CATTTTATTG AAGAAAGACT ATGGAAAATA TTTATACAGC TGTGCTTAGC TCTTCGGTAC 
TTACACAAGG AGAAGAGGAT TGTTCATAGA GATCTGACAC CAAACAACAT TATGTTGGGG 
GATAAGGACA AAGTAACAGT TACTGACTTT GGGCTGGCAA AGCAAAAACA AGAAAACAGT 
AAACTCACGT CTGTTGTTGG AACAATCCTG TATTCCTGCC CTGAGGTACT GAAGAGTGAA 
CCGTACGGGG AGAAAGCTGA TGTCTGGGCA GCAGGCTGCA TTCTTTATCA GATGGCAACT 
CTGAATCCTC CCTTCTACAG CACCAACATG CTCTCCCTGG CTACAAAAAT AGTGGAGGCA 
GTATATGAAC CAGTGCCAGA AGGCATCTAC TCTGAGAAAG TGACAGATAC CATCAGCAGA 
TGCCTCACTC CTGATGCAGA AACCCGCCCA GATATTATAG AAGTCAGCTC AATGATATCA 
GATGTCATGA TGAAGTATTT AGACAACTTA TCTACATCCC AACTGGCTTT GGAAAAGAAA 
CTGGAACGGG AACGGAAGCG TACACAGAGG TACTTTATGG AAGCCAACCG CAATGCTGTC 
ACATGTCACC ATGAGCTGGC TCTGTTATCT CATGAAACCT TTGAGAAGGC AAGCTTGAGT 
AGCAGCAGCA GTGGAGCTGC CAGCCTGAAA AGCGAACTTT CAGAAAGTGC AGACCTACCC 
CCTGAGGGCT TCCAGGCCCC CTGTGGTAAG GATGAAGACA GGGCCTGTGA TGAAATCCTG 
TCGGAGGAGA ACTTCAACTT GGAAAATATT GAGAAAGATA TGTACTCAGA GCTAGATGAT 
GAACTGGACA TTTCGGACAA CTCTAGCAGC TCCAGTTCAA GCCCTTTGAA AGAATCTACA 
TTCAGCATTT TAAAGAGAAG TTTTAGTGCC TCAGGAGGAG AAAGACAATC CCATACAAGG 
GACTTCACTG GTGGAATTGG ATCCAGACCA AGACCAGCTT TGCTGCCTCT TGACCTGCTT 
CTGAAAGTAC CACCCCTCAT GCTCAGGGCC CACATTAAGG AGCTAGAGGC CGAGTTAGTG 
ACGGGGTGGC AGTCCCATAG CCTTCCCGCT GTGATTCTTC GCAATCTCAA AGATCATGGG 
CCACAGATGA GCACATTCTT GTGGCAAGCA TCTGCAGGGA TTGCTGTGTC CCAGAGGAAA 
GTACGACAGA TCAGTGATCC TATTCAGCAG ATCTTAATTC AGCTGCACAA AATCATCTAT 
ATCACCCAGC TCCCTCCAGC TTTGCACCAC AATTTGAAAA GAAGGGTTAT AGAGAGATTC 
AAGAAATCCC TCTTCAGCCA GCAGAGTAAC CCTTGCAATT TGAAATCTGA AATTAAAAAG 
TTATCTCAGG GATCTCCAGA ACCAATCGAG CCCAACTTTT TCACAACAGA TTACCATTTA 
TTACATCATT CATCAGGTGG AAACAGCCTG TCCCCAGACG AACCAGCAGG TCTGCGTACC 
AGCTTTGATT CAGAGGAGGG AATAACATAT GAACAGATGC AGACTGTGAT TGAAGAAGTC 
CTAGAGGAAA GTGGTTATTA CAACTTTACA TCTAACAGAT ATCACTCCTA TCCATGGGGG 
ACCAAGAATC ACCCAACCAA AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK10
Entrez Gene ID	485638
Full Name	NIMA related kinase 10
General protein information	Preferred Names NIMA related kinase 10 Names serine/threonine-protein kinase Nek10 NIMA (never in mitosis gene a)- related kinase 10
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	NEK10	XP_001094156.2
Canis lupus familiaris (dog)	NEK10	XP_005634398.1
Mus musculus (house mouse)	Nek10	NP_001182158.1
Caenorhabditis elegans (roundworm)	nekl-4	NP_498178.3
Bos taurus (cattle)	LOC522335	XP_005222634.1
Rattus norvegicus (Norway rat)	Nek10	XP_006251783.1
Gallus gallus (chicken)	NEK10	XP_001235144.3
Danio rerio (zebrafish)	nek10	XP_002665088.2
Xenopus tropicalis (tropical clawed frog)	nek10	XP_002932455.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK10 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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