Uba2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uba2
Entrez Gene ID	50995
Full Name	ubiquitin-like modifier activating enzyme 2
Synonyms	AA986091,Arx,Sae2,UBA1,Ubl1a2,Uble1b
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 2 Names SUMO-activating enzyme subunit 2 SUMO1 activating enzyme subunit 2 Sumo-1 activating enzyme subunit 2 anthracycline-associated resistance ARX ubiquitin-like 1 (sentrin) activating enzyme E1B ubiquitin-like 1 (sentrin) activating enzyme subunit 2 ubiquitin-like 1-activating enzyme E1B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017322348.2	XP_017177837.1	SUMO-activating enzyme subunit 2 isoform X1
XM_017322348.1	XP_017177837.1	SUMO-activating enzyme subunit 2 isoform X1
NM_016682.2	NP_057891.1	SUMO-activating enzyme subunit 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBA2	NP_001076850.1
Rattus norvegicus (Norway rat)	Uba2	NP_001094049.1
Pan troglodytes (chimpanzee)	UBA2	XP_524210.2
Canis lupus familiaris (dog)	UBA2	XP_533699.3
Mus musculus (house mouse)	Uba2	NP_057891.1
Danio rerio (zebrafish)	uba2	NP_998528.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008637	XP_314735.3
Kluyveromyces lactis	KLLA0E03103g	XP_454085.1
Schizosaccharomyces pombe (fission yeast)	fub2	NP_595945.1
Magnaporthe oryzae (rice blast fungus)	MGG_06733	XP_003709447.1
Arabidopsis thaliana (thale cress)	SAE2	NP_001189570.1
Oryza sativa (rice)	Os07g0586500	NP_001060130.1
	UBA2	XP_001091974.1
Saccharomyces cerevisiae (baker's yeast)	UBA2	NP_010678.3
Eremothecium gossypii	AGOS_AFR138W	NP_985685.1
Neurospora crassa	NCU03526	XP_956805.2
Drosophila melanogaster (fruit fly)	Uba2	NP_524756.2
Homo sapiens (human)	UBA2	NP_005490.1
Gallus gallus (chicken)	UBA2	NP_001025742.1
Xenopus tropicalis (tropical clawed frog)	uba2	NP_001017091.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis.
Ban R, Nishida T, Urano T
Genes to cells : devoted to molecular & cellular mechanisms16(6)652-69(2011 Jun)

Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5.
Costa MW, Lee S, Furtado MB, Xin L, Sparrow DB, Martinez CG, Dunwoodie SL, Kurtenbach E, Mohun T, Rosenthal N, Harvey RP
PloS one6(9)e24812(2011)

Ikaros SUMOylation: switching out of repression.
G?mez-del Arco P, Koipally J, Georgopoulos K
Molecular and cellular biology25(7)2688-97(2005 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Expression and regulation of the mammalian SUMO-1 E1 enzyme.
Azuma Y, Tan SH, Cavenagh MM, Ainsztein AM, Saitoh H, Dasso M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology15(10)1825-7(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Uba2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uba2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16267	NM_016682.2 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$377.30 $539.00
OMu82145	XM_017322348.2 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu82145	XM_017322348.1	Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uba2 ( NM_016682.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057891.1 Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), mRNA.

Price & Availability↑

CloneID	OMu16267
Clone ID Related Accession (Same CDS sequence)	NM_016682.2
Accession Version	NM_016682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX537302.12. On Nov 17, 2006 this sequence version replaced NM_016682.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK163451.1, BC054768.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGCACTGT CGCGGGGGTT GCCCCGGGAG CTGGCCGAGG CGGTGTCCGG CGGCCGGGTG 
CTGGTGGTTG GCGCGGGTGG GATCGGCTGT GAGCTGCTCA AGAACCTGGT GCTCACTGGC 
TTCTCCCACA TCGACCTGAT TGATCTGGAC ACTATCGATG TCAGCAACCT CAACAGGCAG 
TTCTTGTTTC AAAAGAAACA TGTGGGAAGA TCAAAGGCTC AGGTTGCCAA AGAAAGTGTG 
CTCCAGTTCC ACCCCCAAGC CAACATTGAG GCTCACCATG ACAGCATCAT GAACCCAGAC 
TATAATGTGG AGTTTTTTCG ACAGTTTATA TTGGTCATGA ATGCATTAGA TAACAGAGCT 
GCACGAAACC ACGTGAATAG GATGTGTCTG GCCGCTGATG TGCCTCTCAT TGAGAGCGGG 
ACTGCTGGGT ATCTCGGACA GGTGACCACT ATCAAGAAGG GTGTGACTGA GTGTTACGAA 
TGTCACCCTA AGCCTACCCA GAGGACGTTC CCTGGCTGTA CGATTCGGAA CACACCTTCA 
GAACCTATCC ATTGCATCGT ATGGGCCAAG TATTTGTTCA ACCAGCTGTT TGGAGAGGAA 
GATGCTGATC AAGAAGTGTC TCCTGACAGA GCTGACCCTG AGGCTGCTTG GGAACCAACA 
GAGGCTGAAG CTAGAGCTCG AGCATCTAAT GAAGACGGAG ACATTAAGCG TATTTCCACT 
AAGGAATGGG CTAAGTCAAC TGGATATGAT CCAGTTAAAC TTTTTACCAA GCTTTTTAAA 
GATGATATTA GATATCTGCT GACAATGGAC AAACTCTGGC GCAAGAGGAA ACCTCCAGTT 
CCACTGGACT GGGCTGAAGT GCAGAGTCAA GGAGAAGCCA ATGCCGATCA ACAAAATGAG 
CCCCAGTTGG GTTTGAAAGA CCAGCAGGTT CTGGATGTAA AGAGCTATGC GAGTCTGTTT 
TCAAAGAGCA TCGAGACTCT GCGAGTCCAT TTAGCGGAGA AGGGGGATGG AGCTGAGCTC 
ATCTGGGATA AGGACGACCC ACCTGCAATG GATTTTGTTA CATCTGCTGC AAACCTCAGG 
ATGCACATTT TCAGCATGAA TATGAAGAGC AGGTTTGATA TAAAATCAAT GGCAGGGAAT 
ATCATTCCTG CTATAGCTAC TACTAATGCA GTGATCGCCG GCTTGATAGT GTTGGAAGGA 
TTAAAGATTT TATCTGGAAA AATAGACCAG TGTAGAACTA TTTTTTTGAA TAAGCAGCCA 
AACCCAAGAA AGAAGCTCTT GGTGCCCTGT GCACTGGATC CTCCCAACAC CAACTGTTAC 
GTTTGTGCCA GCAAGCCAGA GGTGACTGTG CGGCTGAATG TCCACAAAGT GACTGTCCTC 
ACCTTACAGG ATAAGATAGT GAAAGAAAAG TTTGCTATGG TGGCACCAGA TGTCCAAATT 
GAAGATGGGA AAGGCACAAT CCTGATATCT TCAGAAGAGG GAGAGACAGA AGCCAATAAC 
CCCAAGAAGT TGTCTGATTT TGGAATTAGA AACGGCAGCC GGCTTCAAGC AGACGACTTC 
CTCCAGGACT ATACTTTGCT GATCAACATT CTTCATAGTG AAGATCTTGG AAAGGATGTT 
GAATTTGAAG TAGTTGGTGA CAGCCCTGAA AAAGTGGGGC CAAAACAAGC TGAAGATGCT 
GCCAAAAGCA TTGCCAACGG CAGCGATGAC GGTGCCCAGC CTTCCACCTC CACAGCACAA 
GAGCAAGATG ATGTGCTCAT AGTTGATTCA GACGAAGAAG GCCCTTCAAA TAGCACCGAC 
TGCAGCGGGG ACGACAAGGC CCGCAAGCGG AAGCTGGAAG AGAATGAGGC TGCCAGTACC 
AAGAAGTGCC GCTTGGAGCA GATGGAAGAT CCAGATGACG TCATAGCGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057891.1
CDS	20..1936
Translation	MALSRGLPRELAEAVSGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFHPQANIEAHHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEANADQQNEPQLGLKDQQVLDVKSYASLFSKSIETLRVHLAEKGDGAELIWDKDDPPAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNTNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNPKKLSDFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDSPEKVGPKQAEDAAKSIANGSDDGAQPSTSTAQEQDDVLIVDSDEEGPSNSTDCSGDDKARKRKLEENEAASTKKCRLEQMEDPDDVIALD

Target ORF information:

RefSeq Version	NM_016682.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016682.2

ORF Insert Sequence:

ATGGCACTGT CGCGGGGGTT GCCCCGGGAG CTGGCCGAGG CGGTGTCCGG CGGCCGGGTG 
CTGGTGGTTG GCGCGGGTGG GATCGGCTGT GAGCTGCTCA AGAACCTGGT GCTCACTGGC 
TTCTCCCACA TCGACCTGAT TGATCTGGAC ACTATCGATG TCAGCAACCT CAACAGGCAG 
TTCTTGTTTC AAAAGAAACA TGTGGGAAGA TCAAAGGCTC AGGTTGCCAA AGAAAGTGTG 
CTCCAGTTCC ACCCCCAAGC CAACATTGAG GCTCACCATG ACAGCATCAT GAACCCAGAC 
TATAATGTGG AGTTTTTTCG ACAGTTTATA TTGGTCATGA ATGCATTAGA TAACAGAGCT 
GCACGAAACC ACGTGAATAG GATGTGTCTG GCCGCTGATG TGCCTCTCAT TGAGAGCGGG 
ACTGCTGGGT ATCTCGGACA GGTGACCACT ATCAAGAAGG GTGTGACTGA GTGTTACGAA 
TGTCACCCTA AGCCTACCCA GAGGACGTTC CCTGGCTGTA CGATTCGGAA CACACCTTCA 
GAACCTATCC ATTGCATCGT ATGGGCCAAG TATTTGTTCA ACCAGCTGTT TGGAGAGGAA 
GATGCTGATC AAGAAGTGTC TCCTGACAGA GCTGACCCTG AGGCTGCTTG GGAACCAACA 
GAGGCTGAAG CTAGAGCTCG AGCATCTAAT GAAGACGGAG ACATTAAGCG TATTTCCACT 
AAGGAATGGG CTAAGTCAAC TGGATATGAT CCAGTTAAAC TTTTTACCAA GCTTTTTAAA 
GATGATATTA GATATCTGCT GACAATGGAC AAACTCTGGC GCAAGAGGAA ACCTCCAGTT 
CCACTGGACT GGGCTGAAGT GCAGAGTCAA GGAGAAGCCA ATGCCGATCA ACAAAATGAG 
CCCCAGTTGG GTTTGAAAGA CCAGCAGGTT CTGGATGTAA AGAGCTATGC GAGTCTGTTT 
TCAAAGAGCA TCGAGACTCT GCGAGTCCAT TTAGCGGAGA AGGGGGATGG AGCTGAGCTC 
ATCTGGGATA AGGACGACCC ACCTGCAATG GATTTTGTTA CATCTGCTGC AAACCTCAGG 
ATGCACATTT TCAGCATGAA TATGAAGAGC AGGTTTGATA TAAAATCAAT GGCAGGGAAT 
ATCATTCCTG CTATAGCTAC TACTAATGCA GTGATCGCCG GCTTGATAGT GTTGGAAGGA 
TTAAAGATTT TATCTGGAAA AATAGACCAG TGTAGAACTA TTTTTTTGAA TAAGCAGCCA 
AACCCAAGAA AGAAGCTCTT GGTGCCCTGT GCACTGGATC CTCCCAACAC CAACTGTTAC 
GTTTGTGCCA GCAAGCCAGA GGTGACTGTG CGGCTGAATG TCCACAAAGT GACTGTCCTC 
ACCTTACAGG ATAAGATAGT GAAAGAAAAG TTTGCTATGG TGGCACCAGA TGTCCAAATT 
GAAGATGGGA AAGGCACAAT CCTGATATCT TCAGAAGAGG GAGAGACAGA AGCCAATAAC 
CCCAAGAAGT TGTCTGATTT TGGAATTAGA AACGGCAGCC GGCTTCAAGC AGACGACTTC 
CTCCAGGACT ATACTTTGCT GATCAACATT CTTCATAGTG AAGATCTTGG AAAGGATGTT 
GAATTTGAAG TAGTTGGTGA CAGCCCTGAA AAAGTGGGGC CAAAACAAGC TGAAGATGCT 
GCCAAAAGCA TTGCCAACGG CAGCGATGAC GGTGCCCAGC CTTCCACCTC CACAGCACAA 
GAGCAAGATG ATGTGCTCAT AGTTGATTCA GACGAAGAAG GCCCTTCAAA TAGCACCGAC 
TGCAGCGGGG ACGACAAGGC CCGCAAGCGG AAGCTGGAAG AGAATGAGGC TGCCAGTACC 
AAGAAGTGCC GCTTGGAGCA GATGGAAGAT CCAGATGACG TCATAGCGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba2 ( XM_017322348.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177837.1 Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82145
Clone ID Related Accession (Same CDS sequence)	XM_017322348.2 , XM_017322348.1
Accession Version	XM_017322348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGT CGCGGGGGTT GCCCCGGGAG CTGGCCGAGG CGGTGTCCGG CGGCCGGGTG 
CTGGTGGTTG GCGCGGGTGG GATCGGCTGT GAGCTGCTCA AGAACCTGGT GCTCACTGGC 
TTCTCCCACA TCGACCTGAT TGATCTGGAC ACTATCGATG TCAGCAACCT CAACAGGCAG 
TTCTTGTTTC AAAAGAAACA TGTGGGAAGA TCAAAGGCTC AGGTTGCCAA AGAAAGTGTG 
CTCCAGTTCC ACCCCCAAGC CAACATTGAG GCTCACCATG ACAGCATCAT GAACCCAGAC 
TATAATGTGG AGTTTTTTCG ACAGTTTATA TTGGTCATGA ATGCATTAGA TAACAGAGCT 
GCACGAAACC ACGTGAATAG GATGTGTCTG GCCGCTGATG TGCCTCTCAT TGAGAGCGGG 
ACTGCTGGGT ATCTCGGACA GGTGACCACT ATCAAGAAGG GTGTGACTGA GTGTTACGAA 
TGTCACCCTA AGCCTACCCA GAGGACGTTC CCTGGCTGTA CGATTCGGAA CACACCTTCA 
GAACCTATCC ATTGCATCGT ATGGGCCAAG TATTTGTTCA ACCAGCTGTT TGGAGAGGAA 
GATGCTGATC AAGAAGTGTC TCCTGACAGA GCTGACCCTG AGGCTGCTTG GGAACCAACA 
GAGGCTGAAG CTAGAGCTCG AGCATCTAAT GAAGACGGAG ACATTAAGCG TATTTCCACT 
AAGGAATGGG CTAAGTCAAC TGGATATGAT CCAGTTAAAC TTTTTACCAA GCTTTTTAAA 
GATGATATTA GATATCTGCT GACAATGGAC AAACTCTGGC GCAAGAGGAA ACCTCCAGTT 
CCACTGGACT GGGCTGAAGT GCAGAGTCAA GGAGAAGCCA ATGCCGATCA ACAAAATGAG 
CCCCAGTTGG GTTTGAAAGA CCAGCAGGTT CTGGATGTAA AGAGCTATGC GAGTCTGTTT 
TCAAAGAGCA TCGAGACTCT GCGAGTCCAT TTAGCGGAGA AGGGGGATGG AGCTGAGCTC 
ATCTGGGATA AGGACGACCC ACCTGCAATG GATTTTGTTA CATCTGCTGC AAACCTCAGG 
ATGCACATTT TCAGCATGAA TATGAAGAGC AGGTTTGATA TAAAATCAAT GGCAGGGAAT 
ATCATTCCTG CTATAGCTAC TACTAATGCA GTGATCGCCG GCTTGATAGT GTTGGAAGGA 
TTAAAGATTT TATCTGGAAA AATAGACCAG TGTAGAACTA TTTTTTTGAA TAAGCAGCCA 
AACCCAAGAA AGAAGCTCTT GGTGCCCTGT GCACTGGATC CTCCCAACAC CAACTGTTAC 
GTTTGTGCCA GCAAGCCAGA GGTGACTGTG CGGCTGAATG TCCACAAAGT GACTGTCCTC 
ACCTTACAGG ATAAGATAGT GAAAGAAAAG TTTGCTATGG TGGCACCAGA TGTCCAAATT 
GAAGATGGGA AAGGCACAAT CCTGATATCT TCAGAAGAGG GAGAGACAGA AGCCAATAAC 
CCCAAGAAGT TGTCTGATTT TGGAATTAGA AACGGCAGCC GGCTTCAAGC AGACGACTTC 
CTCCAGGACT ATACTTTGCT GATCAACATT CTTCATAGTG AAGATCTTGG AAAGGATGTT 
GAATTTGAAG TAGTTGGTGA CAGCCCTGAA AAAGTGGGGC CAAAACAAGC TGAAGATGCT 
GCCAAAAGCA TTGCCAACGG CAGCGATGAC GGTGCCCAGC CTTCCACCTC CACAGGGATG 
ATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177837.1
CDS	81..1829
Translation	MALSRGLPRELAEAVSGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFHPQANIEAHHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEANADQQNEPQLGLKDQQVLDVKSYASLFSKSIETLRVHLAEKGDGAELIWDKDDPPAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNTNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNPKKLSDFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDSPEKVGPKQAEDAAKSIANGSDDGAQPSTSTGMIR

Target ORF information:

RefSeq Version	XM_017322348.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322348.2

ORF Insert Sequence:

ATGGCACTGT CGCGGGGGTT GCCCCGGGAG CTGGCCGAGG CGGTGTCCGG CGGCCGGGTG 
CTGGTGGTTG GCGCGGGTGG GATCGGCTGT GAGCTGCTCA AGAACCTGGT GCTCACTGGC 
TTCTCCCACA TCGACCTGAT TGATCTGGAC ACTATCGATG TCAGCAACCT CAACAGGCAG 
TTCTTGTTTC AAAAGAAACA TGTGGGAAGA TCAAAGGCTC AGGTTGCCAA AGAAAGTGTG 
CTCCAGTTCC ACCCCCAAGC CAACATTGAG GCTCACCATG ACAGCATCAT GAACCCAGAC 
TATAATGTGG AGTTTTTTCG ACAGTTTATA TTGGTCATGA ATGCATTAGA TAACAGAGCT 
GCACGAAACC ACGTGAATAG GATGTGTCTG GCCGCTGATG TGCCTCTCAT TGAGAGCGGG 
ACTGCTGGGT ATCTCGGACA GGTGACCACT ATCAAGAAGG GTGTGACTGA GTGTTACGAA 
TGTCACCCTA AGCCTACCCA GAGGACGTTC CCTGGCTGTA CGATTCGGAA CACACCTTCA 
GAACCTATCC ATTGCATCGT ATGGGCCAAG TATTTGTTCA ACCAGCTGTT TGGAGAGGAA 
GATGCTGATC AAGAAGTGTC TCCTGACAGA GCTGACCCTG AGGCTGCTTG GGAACCAACA 
GAGGCTGAAG CTAGAGCTCG AGCATCTAAT GAAGACGGAG ACATTAAGCG TATTTCCACT 
AAGGAATGGG CTAAGTCAAC TGGATATGAT CCAGTTAAAC TTTTTACCAA GCTTTTTAAA 
GATGATATTA GATATCTGCT GACAATGGAC AAACTCTGGC GCAAGAGGAA ACCTCCAGTT 
CCACTGGACT GGGCTGAAGT GCAGAGTCAA GGAGAAGCCA ATGCCGATCA ACAAAATGAG 
CCCCAGTTGG GTTTGAAAGA CCAGCAGGTT CTGGATGTAA AGAGCTATGC GAGTCTGTTT 
TCAAAGAGCA TCGAGACTCT GCGAGTCCAT TTAGCGGAGA AGGGGGATGG AGCTGAGCTC 
ATCTGGGATA AGGACGACCC ACCTGCAATG GATTTTGTTA CATCTGCTGC AAACCTCAGG 
ATGCACATTT TCAGCATGAA TATGAAGAGC AGGTTTGATA TAAAATCAAT GGCAGGGAAT 
ATCATTCCTG CTATAGCTAC TACTAATGCA GTGATCGCCG GCTTGATAGT GTTGGAAGGA 
TTAAAGATTT TATCTGGAAA AATAGACCAG TGTAGAACTA TTTTTTTGAA TAAGCAGCCA 
AACCCAAGAA AGAAGCTCTT GGTGCCCTGT GCACTGGATC CTCCCAACAC CAACTGTTAC 
GTTTGTGCCA GCAAGCCAGA GGTGACTGTG CGGCTGAATG TCCACAAAGT GACTGTCCTC 
ACCTTACAGG ATAAGATAGT GAAAGAAAAG TTTGCTATGG TGGCACCAGA TGTCCAAATT 
GAAGATGGGA AAGGCACAAT CCTGATATCT TCAGAAGAGG GAGAGACAGA AGCCAATAAC 
CCCAAGAAGT TGTCTGATTT TGGAATTAGA AACGGCAGCC GGCTTCAAGC AGACGACTTC 
CTCCAGGACT ATACTTTGCT GATCAACATT CTTCATAGTG AAGATCTTGG AAAGGATGTT 
GAATTTGAAG TAGTTGGTGA CAGCCCTGAA AAAGTGGGGC CAAAACAAGC TGAAGATGCT 
GCCAAAAGCA TTGCCAACGG CAGCGATGAC GGTGCCCAGC CTTCCACCTC CACAGGGATG 
ATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba2 ( XM_017322348.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177837.1 Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82145
Clone ID Related Accession (Same CDS sequence)	XM_017322348.2 , XM_017322348.1
Accession Version	XM_017322348.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGT CGCGGGGGTT GCCCCGGGAG CTGGCCGAGG CGGTGTCCGG CGGCCGGGTG 
CTGGTGGTTG GCGCGGGTGG GATCGGCTGT GAGCTGCTCA AGAACCTGGT GCTCACTGGC 
TTCTCCCACA TCGACCTGAT TGATCTGGAC ACTATCGATG TCAGCAACCT CAACAGGCAG 
TTCTTGTTTC AAAAGAAACA TGTGGGAAGA TCAAAGGCTC AGGTTGCCAA AGAAAGTGTG 
CTCCAGTTCC ACCCCCAAGC CAACATTGAG GCTCACCATG ACAGCATCAT GAACCCAGAC 
TATAATGTGG AGTTTTTTCG ACAGTTTATA TTGGTCATGA ATGCATTAGA TAACAGAGCT 
GCACGAAACC ACGTGAATAG GATGTGTCTG GCCGCTGATG TGCCTCTCAT TGAGAGCGGG 
ACTGCTGGGT ATCTCGGACA GGTGACCACT ATCAAGAAGG GTGTGACTGA GTGTTACGAA 
TGTCACCCTA AGCCTACCCA GAGGACGTTC CCTGGCTGTA CGATTCGGAA CACACCTTCA 
GAACCTATCC ATTGCATCGT ATGGGCCAAG TATTTGTTCA ACCAGCTGTT TGGAGAGGAA 
GATGCTGATC AAGAAGTGTC TCCTGACAGA GCTGACCCTG AGGCTGCTTG GGAACCAACA 
GAGGCTGAAG CTAGAGCTCG AGCATCTAAT GAAGACGGAG ACATTAAGCG TATTTCCACT 
AAGGAATGGG CTAAGTCAAC TGGATATGAT CCAGTTAAAC TTTTTACCAA GCTTTTTAAA 
GATGATATTA GATATCTGCT GACAATGGAC AAACTCTGGC GCAAGAGGAA ACCTCCAGTT 
CCACTGGACT GGGCTGAAGT GCAGAGTCAA GGAGAAGCCA ATGCCGATCA ACAAAATGAG 
CCCCAGTTGG GTTTGAAAGA CCAGCAGGTT CTGGATGTAA AGAGCTATGC GAGTCTGTTT 
TCAAAGAGCA TCGAGACTCT GCGAGTCCAT TTAGCGGAGA AGGGGGATGG AGCTGAGCTC 
ATCTGGGATA AGGACGACCC ACCTGCAATG GATTTTGTTA CATCTGCTGC AAACCTCAGG 
ATGCACATTT TCAGCATGAA TATGAAGAGC AGGTTTGATA TAAAATCAAT GGCAGGGAAT 
ATCATTCCTG CTATAGCTAC TACTAATGCA GTGATCGCCG GCTTGATAGT GTTGGAAGGA 
TTAAAGATTT TATCTGGAAA AATAGACCAG TGTAGAACTA TTTTTTTGAA TAAGCAGCCA 
AACCCAAGAA AGAAGCTCTT GGTGCCCTGT GCACTGGATC CTCCCAACAC CAACTGTTAC 
GTTTGTGCCA GCAAGCCAGA GGTGACTGTG CGGCTGAATG TCCACAAAGT GACTGTCCTC 
ACCTTACAGG ATAAGATAGT GAAAGAAAAG TTTGCTATGG TGGCACCAGA TGTCCAAATT 
GAAGATGGGA AAGGCACAAT CCTGATATCT TCAGAAGAGG GAGAGACAGA AGCCAATAAC 
CCCAAGAAGT TGTCTGATTT TGGAATTAGA AACGGCAGCC GGCTTCAAGC AGACGACTTC 
CTCCAGGACT ATACTTTGCT GATCAACATT CTTCATAGTG AAGATCTTGG AAAGGATGTT 
GAATTTGAAG TAGTTGGTGA CAGCCCTGAA AAAGTGGGGC CAAAACAAGC TGAAGATGCT 
GCCAAAAGCA TTGCCAACGG CAGCGATGAC GGTGCCCAGC CTTCCACCTC CACAGGGATG 
ATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177837.1
CDS	76..1824
Translation	MALSRGLPRELAEAVSGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFHPQANIEAHHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEANADQQNEPQLGLKDQQVLDVKSYASLFSKSIETLRVHLAEKGDGAELIWDKDDPPAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNTNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNPKKLSDFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDSPEKVGPKQAEDAAKSIANGSDDGAQPSTSTGMIR

Target ORF information:

RefSeq Version	XM_017322348.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 2 (Uba2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322348.1

ORF Insert Sequence:

ATGGCACTGT CGCGGGGGTT GCCCCGGGAG CTGGCCGAGG CGGTGTCCGG CGGCCGGGTG 
CTGGTGGTTG GCGCGGGTGG GATCGGCTGT GAGCTGCTCA AGAACCTGGT GCTCACTGGC 
TTCTCCCACA TCGACCTGAT TGATCTGGAC ACTATCGATG TCAGCAACCT CAACAGGCAG 
TTCTTGTTTC AAAAGAAACA TGTGGGAAGA TCAAAGGCTC AGGTTGCCAA AGAAAGTGTG 
CTCCAGTTCC ACCCCCAAGC CAACATTGAG GCTCACCATG ACAGCATCAT GAACCCAGAC 
TATAATGTGG AGTTTTTTCG ACAGTTTATA TTGGTCATGA ATGCATTAGA TAACAGAGCT 
GCACGAAACC ACGTGAATAG GATGTGTCTG GCCGCTGATG TGCCTCTCAT TGAGAGCGGG 
ACTGCTGGGT ATCTCGGACA GGTGACCACT ATCAAGAAGG GTGTGACTGA GTGTTACGAA 
TGTCACCCTA AGCCTACCCA GAGGACGTTC CCTGGCTGTA CGATTCGGAA CACACCTTCA 
GAACCTATCC ATTGCATCGT ATGGGCCAAG TATTTGTTCA ACCAGCTGTT TGGAGAGGAA 
GATGCTGATC AAGAAGTGTC TCCTGACAGA GCTGACCCTG AGGCTGCTTG GGAACCAACA 
GAGGCTGAAG CTAGAGCTCG AGCATCTAAT GAAGACGGAG ACATTAAGCG TATTTCCACT 
AAGGAATGGG CTAAGTCAAC TGGATATGAT CCAGTTAAAC TTTTTACCAA GCTTTTTAAA 
GATGATATTA GATATCTGCT GACAATGGAC AAACTCTGGC GCAAGAGGAA ACCTCCAGTT 
CCACTGGACT GGGCTGAAGT GCAGAGTCAA GGAGAAGCCA ATGCCGATCA ACAAAATGAG 
CCCCAGTTGG GTTTGAAAGA CCAGCAGGTT CTGGATGTAA AGAGCTATGC GAGTCTGTTT 
TCAAAGAGCA TCGAGACTCT GCGAGTCCAT TTAGCGGAGA AGGGGGATGG AGCTGAGCTC 
ATCTGGGATA AGGACGACCC ACCTGCAATG GATTTTGTTA CATCTGCTGC AAACCTCAGG 
ATGCACATTT TCAGCATGAA TATGAAGAGC AGGTTTGATA TAAAATCAAT GGCAGGGAAT 
ATCATTCCTG CTATAGCTAC TACTAATGCA GTGATCGCCG GCTTGATAGT GTTGGAAGGA 
TTAAAGATTT TATCTGGAAA AATAGACCAG TGTAGAACTA TTTTTTTGAA TAAGCAGCCA 
AACCCAAGAA AGAAGCTCTT GGTGCCCTGT GCACTGGATC CTCCCAACAC CAACTGTTAC 
GTTTGTGCCA GCAAGCCAGA GGTGACTGTG CGGCTGAATG TCCACAAAGT GACTGTCCTC 
ACCTTACAGG ATAAGATAGT GAAAGAAAAG TTTGCTATGG TGGCACCAGA TGTCCAAATT 
GAAGATGGGA AAGGCACAAT CCTGATATCT TCAGAAGAGG GAGAGACAGA AGCCAATAAC 
CCCAAGAAGT TGTCTGATTT TGGAATTAGA AACGGCAGCC GGCTTCAAGC AGACGACTTC 
CTCCAGGACT ATACTTTGCT GATCAACATT CTTCATAGTG AAGATCTTGG AAAGGATGTT 
GAATTTGAAG TAGTTGGTGA CAGCCCTGAA AAAGTGGGGC CAAAACAAGC TGAAGATGCT 
GCCAAAAGCA TTGCCAACGG CAGCGATGAC GGTGCCCAGC CTTCCACCTC CACAGGGATG 
ATTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis.
Genes to cells : devoted to molecular & cellular mechanisms16(6)652-69(2011 Jun)
Ban R,Nishida T,Urano T

Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5.
PloS one6(9)e24812(2011)
Costa MW,Lee S,Furtado MB,Xin L,Sparrow DB,Martinez CG,Dunwoodie SL,Kurtenbach E,Mohun T,Rosenthal N,Harvey RP

Ikaros SUMOylation: switching out of repression.
Molecular and cellular biology25(7)2688-97(2005 Apr)
G?mez-del Arco P,Koipally J,Georgopoulos K

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Expression and regulation of the mammalian SUMO-1 E1 enzyme.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology15(10)1825-7(2001 Aug)
Azuma Y,Tan SH,Cavenagh MM,Ainsztein AM,Saitoh H,Dasso M

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Uba2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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