UBA2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA2
Entrez Gene ID	406672
Full Name	ubiquitin-like modifier activating enzyme 2
Synonyms	sae2,sae2b,uble1b,wu:fi17g06,zgc:66354
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 2 Names SUMO-activating enzyme subunit 2 SUMO1 activating enzyme subunit 2 SUMO1 activating enzyme subunit 2b ubiquitin-like 1 (sentrin) activating enzyme E1B ubiquitin-like 1-activating enzyme E1B
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213363.1	NP_998528.1	SUMO-activating enzyme subunit 2
XM_009297857.2	XP_009296132.1	SUMO-activating enzyme subunit 2 isoform X1
XM_009297857.3	XP_009296132.1	SUMO-activating enzyme subunit 2 isoform X1
XM_009297858.3	XP_009296133.1	SUMO-activating enzyme subunit 2 isoform X2
XM_009297858.2	XP_009296133.1	SUMO-activating enzyme subunit 2 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBA2	NP_001076850.1
Rattus norvegicus (Norway rat)	Uba2	NP_001094049.1
Pan troglodytes (chimpanzee)	UBA2	XP_524210.2
Canis lupus familiaris (dog)	UBA2	XP_533699.3
Mus musculus (house mouse)	Uba2	NP_057891.1
Danio rerio (zebrafish)	uba2	NP_998528.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008637	XP_314735.3
Kluyveromyces lactis	KLLA0E03103g	XP_454085.1
Schizosaccharomyces pombe (fission yeast)	fub2	NP_595945.1
Magnaporthe oryzae (rice blast fungus)	MGG_06733	XP_003709447.1
Arabidopsis thaliana (thale cress)	SAE2	NP_001189570.1
Oryza sativa (rice)	Os07g0586500	NP_001060130.1
	UBA2	XP_001091974.1
Saccharomyces cerevisiae (baker's yeast)	UBA2	NP_010678.3
Eremothecium gossypii	AGOS_AFR138W	NP_985685.1
Neurospora crassa	NCU03526	XP_956805.2
Drosophila melanogaster (fruit fly)	Uba2	NP_524756.2
Homo sapiens (human)	UBA2	NP_005490.1
Gallus gallus (chicken)	UBA2	NP_001025742.1
Xenopus tropicalis (tropical clawed frog)	uba2	NP_001017091.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Parp inhibition prevents ten-eleven translocase enzyme activation and hyperglycemia-induced DNA demethylation.
Dhliwayo N, Sarras MP Jr, Luczkowski E, Mason SM, Intine RV
Diabetes63(9)3069-76(2014 Sep)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UBA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37796	NM_213363.1 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40894	XM_009297857.3 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40895	XM_009297858.3 Latest version!	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa40894	XM_009297857.2	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40895	XM_009297858.2	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA2 ( NM_213363.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_998528.1 Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), mRNA.

Price & Availability↑

CloneID	ODa37796
Clone ID Related Accession (Same CDS sequence)	NM_213363.1
Accession Version	NM_213363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Danio rerio(zebrafish)
Product	SUMO-activating enzyme subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055614.1. ##Evidence-Data-START## Transcript exon combination :: BC055614.1, BC164556.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATTAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGGTTCTC AGGAGCAGGT AATTGGCTCA 
GGGCTGAAGG ACCAGCAGGT TTTGGGAGTT CAGGGTTATG CTCAACTCTT CCAGCATAGT 
GTAGAAACTC TCCGATCTCA GCTGAAAGAG AAGGGTGACG GTGCAGAGCT CGTCTGGGAC 
AAGGACGATC CTCCAGCCAT GGACTTTGTT ACAGCAGCTT CAAACTTGCG TATGAATGTA 
TTCAGCATGA ACATGAAAAG CCGCTTTGAT GTCAAATCCA TGGCAGGCAA CATCATTCCA 
GCTATAGCCA CCACTAACGC CGTCATTGCC GGTCTCATTG TTCTGGAAGC ACTGAAGATT 
CTCAACTCAG ACTTTGAACA GTGTCGCACG ATCTTCCTAA ACAAACAGCC AAACCCCAGG 
AAAAAGCTGC TCGTCCCGTG TGCGCTGGAC CCTCCAAATG CCAGCTGTTA TGTTTGTGCC 
AGCAAACCCG AGGTCACAGT AAAGCTTAAC GTGCACAAGA CCATGGTGCA AGCTCTTCAG 
GACAAGATAC TGAAGGAGAA GTTTGGCATG GTGGCACCAG ATGTGCAGAT TGAGGACGGA 
AAGGGAACGA TCCTCATCTC TTCAGAGGAA GGAGAGACTG AAGCAAACAA TAACAAGTTT 
TTGTCTGACT TTGGGATTCG GAATGGGAGC CATCTTCAAG CTGACGACTT CCTACAGGAT 
TACACTCTTC TGGTCAATGT AATTCACAGT GAGGAGTTGG AAAAGGATGT TGAGTTTGAA 
GTAGTCGGTG ATGCCCCGGA TAAAGCGCCT GCTCCAAGTG CCCCAGAAGA AGGGAAAAAC 
ATTGCCAATG GGAATAAAGA TTCTGCCCAG CCATCTACCT CATCAAAAGC AGCTGTAGAG 
GACGACGATG TCCTTCTCGT AGACTCTGAT GAAGAGCCGT CATCAAGCAC CATGGACACA 
GAGTCCAGTA ACCGCAAGAG GAAACATCAT GATGCTGAAA CAGACGATGC TTCATCTAAG 
CGCAAACGCC TGGACCAGCA GCCGGCCGAT GATGATGATG AAGACATCAT CGCTCTAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998528.1
CDS	18..1940
Translation	MAELVGPLRKQLADSLSSCRVLVVGAGGIGCELLKNLVLTGFKNIEVIDLDTIDVSNLNRQFLFQKKHVGKSKAQVAKESVLRFCPSANITAYHDSIMNPDYNVEFFRNFQLVMNALDNRAARNHVNRMCLAADIPLIESGTAGYLGQVTVIKKGQTECYECQPKPTQKTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDTADPEAAWNPADAAARATASDQDGDIKRVSTKEWARSTGYDPIKLFNKLFKDDIMYLLTMDKLWKKRKAPLPLEWEEINQLGSQEQVIGSGLKDQQVLGVQGYAQLFQHSVETLRSQLKEKGDGAELVWDKDDPPAMDFVTAASNLRMNVFSMNMKSRFDVKSMAGNIIPAIATTNAVIAGLIVLEALKILNSDFEQCRTIFLNKQPNPRKKLLVPCALDPPNASCYVCASKPEVTVKLNVHKTMVQALQDKILKEKFGMVAPDVQIEDGKGTILISSEEGETEANNNKFLSDFGIRNGSHLQADDFLQDYTLLVNVIHSEELEKDVEFEVVGDAPDKAPAPSAPEEGKNIANGNKDSAQPSTSSKAAVEDDDVLLVDSDEEPSSSTMDTESSNRKRKHHDAETDDASSKRKRLDQQPADDDDEDIIALD

Target ORF information:

RefSeq Version	NM_213363.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213363.1

ORF Insert Sequence:

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATTAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGGTTCTC AGGAGCAGGT AATTGGCTCA 
GGGCTGAAGG ACCAGCAGGT TTTGGGAGTT CAGGGTTATG CTCAACTCTT CCAGCATAGT 
GTAGAAACTC TCCGATCTCA GCTGAAAGAG AAGGGTGACG GTGCAGAGCT CGTCTGGGAC 
AAGGACGATC CTCCAGCCAT GGACTTTGTT ACAGCAGCTT CAAACTTGCG TATGAATGTA 
TTCAGCATGA ACATGAAAAG CCGCTTTGAT GTCAAATCCA TGGCAGGCAA CATCATTCCA 
GCTATAGCCA CCACTAACGC CGTCATTGCC GGTCTCATTG TTCTGGAAGC ACTGAAGATT 
CTCAACTCAG ACTTTGAACA GTGTCGCACG ATCTTCCTAA ACAAACAGCC AAACCCCAGG 
AAAAAGCTGC TCGTCCCGTG TGCGCTGGAC CCTCCAAATG CCAGCTGTTA TGTTTGTGCC 
AGCAAACCCG AGGTCACAGT AAAGCTTAAC GTGCACAAGA CCATGGTGCA AGCTCTTCAG 
GACAAGATAC TGAAGGAGAA GTTTGGCATG GTGGCACCAG ATGTGCAGAT TGAGGACGGA 
AAGGGAACGA TCCTCATCTC TTCAGAGGAA GGAGAGACTG AAGCAAACAA TAACAAGTTT 
TTGTCTGACT TTGGGATTCG GAATGGGAGC CATCTTCAAG CTGACGACTT CCTACAGGAT 
TACACTCTTC TGGTCAATGT AATTCACAGT GAGGAGTTGG AAAAGGATGT TGAGTTTGAA 
GTAGTCGGTG ATGCCCCGGA TAAAGCGCCT GCTCCAAGTG CCCCAGAAGA AGGGAAAAAC 
ATTGCCAATG GGAATAAAGA TTCTGCCCAG CCATCTACCT CATCAAAAGC AGCTGTAGAG 
GACGACGATG TCCTTCTCGT AGACTCTGAT GAAGAGCCGT CATCAAGCAC CATGGACACA 
GAGTCCAGTA ACCGCAAGAG GAAACATCAT GATGCTGAAA CAGACGATGC TTCATCTAAG 
CGCAAACGCC TGGACCAGCA GCCGGCCGAT GATGATGATG AAGACATCAT CGCTCTAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA2 ( XM_009297857.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296132.1 Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40894
Clone ID Related Accession (Same CDS sequence)	XM_009297857.2 , XM_009297857.3
Accession Version	XM_009297857.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SUMO-activating enzyme subunit 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCAGCTGTA 
GAGGACGACG ATGTCCTTCT CGTAGACTCT GATGAAGAGC CGTCATCAAG CACCATGGAC 
ACAGAGTCCA GTAACCGCAA GAGGAAACAT CATGATGCTG AAACAGACGA TGCTTCATCT 
AAGCGCAAAC GCCTGGACCA GCAGCCGGCC GATGATGATG ATGAAGACAT CATCGCTCTA 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296132.1
CDS	69..1994
Translation	MAELVGPLRKQLADSLSSCRVLVVGAGGIGCELLKNLVLTGFKNIEVIDLDTIDVSNLNRQFLFQKKHVGKSKAQVAKESVLRFCPSANITAYHDSIMNPDYNVEFFRNFQLVMNALDNRAARNHVNRMCLAADIPLIESGTAGYLGQVTVIKKGQTECYECQPKPTQKTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDTADPEAAWNPADAAARATASDQDGDIKRVSTKEWARSTGYDPIKLFNKLFKDDIMYLLTMDKLWKKRKAPLPLEWEEINQLAGSQEQVIGSGLKDQQVLGVQGYAQLFQHSVETLRSQLKEKGDGAELVWDKDDPPAMDFVTAASNLRMNVFSMNMKSRFDVKSMAGNIIPAIATTNAVIAGLIVLEALKILNSDFEQCRTIFLNKQPNPRKKLLVPCALDPPNASCYVCASKPEVTVKLNVHKTMVQALQDKILKEKFGMVAPDVQIEDGKGTILISSEEGETEANNNKFLSDFGIRNGSRLQADDFLQDYTLLVNVIHSEELEKDVEFEVVGDAPDKAPAPSAPEEGKNIANGNKDSAQPSTSSKAAVEDDDVLLVDSDEEPSSSTMDTESSNRKRKHHDAETDDASSKRKRLDQQPADDDDEDIIALD

Target ORF information:

RefSeq Version	XM_009297857.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297857.2

ORF Insert Sequence:

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCAGCTGTA 
GAGGACGACG ATGTCCTTCT CGTAGACTCT GATGAAGAGC CGTCATCAAG CACCATGGAC 
ACAGAGTCCA GTAACCGCAA GAGGAAACAT CATGATGCTG AAACAGACGA TGCTTCATCT 
AAGCGCAAAC GCCTGGACCA GCAGCCGGCC GATGATGATG ATGAAGACAT CATCGCTCTA 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA2 ( XM_009297857.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296132.1 Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40894
Clone ID Related Accession (Same CDS sequence)	XM_009297857.2 , XM_009297857.3
Accession Version	XM_009297857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SUMO-activating enzyme subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCAGCTGTA 
GAGGACGACG ATGTCCTTCT CGTAGACTCT GATGAAGAGC CGTCATCAAG CACCATGGAC 
ACAGAGTCCA GTAACCGCAA GAGGAAACAT CATGATGCTG AAACAGACGA TGCTTCATCT 
AAGCGCAAAC GCCTGGACCA GCAGCCGGCC GATGATGATG ATGAAGACAT CATCGCTCTA 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296132.1
CDS	70..1995
Translation	MAELVGPLRKQLADSLSSCRVLVVGAGGIGCELLKNLVLTGFKNIEVIDLDTIDVSNLNRQFLFQKKHVGKSKAQVAKESVLRFCPSANITAYHDSIMNPDYNVEFFRNFQLVMNALDNRAARNHVNRMCLAADIPLIESGTAGYLGQVTVIKKGQTECYECQPKPTQKTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDTADPEAAWNPADAAARATASDQDGDIKRVSTKEWARSTGYDPIKLFNKLFKDDIMYLLTMDKLWKKRKAPLPLEWEEINQLAGSQEQVIGSGLKDQQVLGVQGYAQLFQHSVETLRSQLKEKGDGAELVWDKDDPPAMDFVTAASNLRMNVFSMNMKSRFDVKSMAGNIIPAIATTNAVIAGLIVLEALKILNSDFEQCRTIFLNKQPNPRKKLLVPCALDPPNASCYVCASKPEVTVKLNVHKTMVQALQDKILKEKFGMVAPDVQIEDGKGTILISSEEGETEANNNKFLSDFGIRNGSRLQADDFLQDYTLLVNVIHSEELEKDVEFEVVGDAPDKAPAPSAPEEGKNIANGNKDSAQPSTSSKAAVEDDDVLLVDSDEEPSSSTMDTESSNRKRKHHDAETDDASSKRKRLDQQPADDDDEDIIALD

Target ORF information:

RefSeq Version	XM_009297857.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297857.3

ORF Insert Sequence:

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCAGCTGTA 
GAGGACGACG ATGTCCTTCT CGTAGACTCT GATGAAGAGC CGTCATCAAG CACCATGGAC 
ACAGAGTCCA GTAACCGCAA GAGGAAACAT CATGATGCTG AAACAGACGA TGCTTCATCT 
AAGCGCAAAC GCCTGGACCA GCAGCCGGCC GATGATGATG ATGAAGACAT CATCGCTCTA 
GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA2 ( XM_009297858.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296133.1 Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40895
Clone ID Related Accession (Same CDS sequence)	XM_009297858.3 , XM_009297858.2
Accession Version	XM_009297858.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SUMO-activating enzyme subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCTGTAGAG 
GACGACGATG TCCTTCTCGT AGACTCTGAT GAAGAGCCGT CATCAAGCAC CATGGACACA 
GAGTCCAGTA ACCGCAAGAG GAAACATCAT GATGCTGAAA CAGACGATGC TTCATCTAAG 
CGCAAACGCC TGGACCAGCA GCCGGCCGAT GATGATGATG AAGACATCAT CGCTCTAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296133.1
CDS	71..1993
Translation	MAELVGPLRKQLADSLSSCRVLVVGAGGIGCELLKNLVLTGFKNIEVIDLDTIDVSNLNRQFLFQKKHVGKSKAQVAKESVLRFCPSANITAYHDSIMNPDYNVEFFRNFQLVMNALDNRAARNHVNRMCLAADIPLIESGTAGYLGQVTVIKKGQTECYECQPKPTQKTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDTADPEAAWNPADAAARATASDQDGDIKRVSTKEWARSTGYDPIKLFNKLFKDDIMYLLTMDKLWKKRKAPLPLEWEEINQLAGSQEQVIGSGLKDQQVLGVQGYAQLFQHSVETLRSQLKEKGDGAELVWDKDDPPAMDFVTAASNLRMNVFSMNMKSRFDVKSMAGNIIPAIATTNAVIAGLIVLEALKILNSDFEQCRTIFLNKQPNPRKKLLVPCALDPPNASCYVCASKPEVTVKLNVHKTMVQALQDKILKEKFGMVAPDVQIEDGKGTILISSEEGETEANNNKFLSDFGIRNGSRLQADDFLQDYTLLVNVIHSEELEKDVEFEVVGDAPDKAPAPSAPEEGKNIANGNKDSAQPSTSSKAVEDDDVLLVDSDEEPSSSTMDTESSNRKRKHHDAETDDASSKRKRLDQQPADDDDEDIIALD

Target ORF information:

RefSeq Version	XM_009297858.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297858.3

ORF Insert Sequence:

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCTGTAGAG 
GACGACGATG TCCTTCTCGT AGACTCTGAT GAAGAGCCGT CATCAAGCAC CATGGACACA 
GAGTCCAGTA ACCGCAAGAG GAAACATCAT GATGCTGAAA CAGACGATGC TTCATCTAAG 
CGCAAACGCC TGGACCAGCA GCCGGCCGAT GATGATGATG AAGACATCAT CGCTCTAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA2 ( XM_009297858.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296133.1 Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40895
Clone ID Related Accession (Same CDS sequence)	XM_009297858.3 , XM_009297858.2
Accession Version	XM_009297858.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SUMO-activating enzyme subunit 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCTGTAGAG 
GACGACGATG TCCTTCTCGT AGACTCTGAT GAAGAGCCGT CATCAAGCAC CATGGACACA 
GAGTCCAGTA ACCGCAAGAG GAAACATCAT GATGCTGAAA CAGACGATGC TTCATCTAAG 
CGCAAACGCC TGGACCAGCA GCCGGCCGAT GATGATGATG AAGACATCAT CGCTCTAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296133.1
CDS	69..1991
Translation	MAELVGPLRKQLADSLSSCRVLVVGAGGIGCELLKNLVLTGFKNIEVIDLDTIDVSNLNRQFLFQKKHVGKSKAQVAKESVLRFCPSANITAYHDSIMNPDYNVEFFRNFQLVMNALDNRAARNHVNRMCLAADIPLIESGTAGYLGQVTVIKKGQTECYECQPKPTQKTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDTADPEAAWNPADAAARATASDQDGDIKRVSTKEWARSTGYDPIKLFNKLFKDDIMYLLTMDKLWKKRKAPLPLEWEEINQLAGSQEQVIGSGLKDQQVLGVQGYAQLFQHSVETLRSQLKEKGDGAELVWDKDDPPAMDFVTAASNLRMNVFSMNMKSRFDVKSMAGNIIPAIATTNAVIAGLIVLEALKILNSDFEQCRTIFLNKQPNPRKKLLVPCALDPPNASCYVCASKPEVTVKLNVHKTMVQALQDKILKEKFGMVAPDVQIEDGKGTILISSEEGETEANNNKFLSDFGIRNGSRLQADDFLQDYTLLVNVIHSEELEKDVEFEVVGDAPDKAPAPSAPEEGKNIANGNKDSAQPSTSSKAVEDDDVLLVDSDEEPSSSTMDTESSNRKRKHHDAETDDASSKRKRLDQQPADDDDEDIIALD

Target ORF information:

RefSeq Version	XM_009297858.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin-like modifier activating enzyme 2 (uba2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297858.2

ORF Insert Sequence:

ATGGCAGAAC TAGTCGGTCC TCTCCGAAAA CAGCTCGCTG ACTCCCTCTC CTCCTGCCGG 
GTGCTGGTGG TCGGGGCGGG AGGCATCGGC TGCGAGCTCC TGAAAAACCT GGTGCTCACC 
GGCTTTAAGA ATATTGAGGT GATTGACCTG GATACCATTG ATGTCAGCAA CCTCAACAGA 
CAATTTCTGT TTCAGAAGAA ACATGTTGGA AAGTCTAAAG CTCAGGTTGC CAAGGAGAGC 
GTGCTTCGGT TTTGTCCATC TGCAAACATC ACTGCCTATC ACGACAGCAT CATGAACCCA 
GATTACAATG TGGAGTTCTT CAGGAATTTC CAGCTTGTAA TGAATGCTTT GGACAACAGA 
GCGGCCAGAA ATCATGTGAA CCGAATGTGT CTGGCAGCTG ACATTCCCCT TATTGAGAGC 
GGCACGGCTG GCTATCTGGG ACAAGTCACG GTTATCAAGA AGGGTCAGAC AGAATGCTAC 
GAGTGCCAGC CTAAACCAAC ACAGAAAACC TTCCCAGGTT GCACCATTCG CAACACGCCC 
TCAGAACCCA TCCACTGCAT TGTGTGGGCT AAATATCTCT TCAATCAGCT GTTTGGTGAG 
GAGGATGCAG ATCAGGAAGT ATCGCCTGAC ACTGCTGACC CTGAAGCCGC CTGGAATCCT 
GCAGATGCAG CAGCTCGAGC CACAGCATCC GATCAGGATG GAGACATCAA ACGAGTGTCC 
ACTAAAGAGT GGGCTCGTTC CACAGGCTAC GACCCTATCA AACTCTTCAA CAAGCTTTTT 
AAAGATGACA TAATGTATCT GCTGACCATG GATAAGCTTT GGAAAAAAAG AAAAGCCCCG 
TTACCACTAG AATGGGAAGA AATCAACCAG CTTGCAGGTT CTCAGGAGCA GGTAATTGGC 
TCAGGGCTGA AGGACCAGCA GGTTTTGGGA GTTCAGGGTT ATGCTCAACT CTTCCAGCAT 
AGTGTAGAAA CTCTACGATC TCAGCTGAAA GAGAAGGGTG ACGGTGCAGA GCTCGTCTGG 
GACAAGGACG ATCCTCCAGC CATGGACTTT GTTACAGCAG CTTCAAACTT GCGTATGAAT 
GTATTCAGCA TGAACATGAA AAGCCGCTTT GATGTCAAAT CCATGGCAGG CAACATCATT 
CCAGCTATAG CCACCACTAA CGCCGTCATT GCCGGTCTCA TTGTTCTGGA AGCACTGAAG 
ATTCTCAACT CAGACTTTGA ACAGTGTCGC ACGATCTTCC TAAACAAACA GCCAAACCCC 
AGGAAAAAGC TGCTCGTCCC GTGTGCGCTG GACCCTCCAA ATGCCAGCTG TTATGTTTGT 
GCCAGCAAAC CCGAGGTCAC AGTAAAGCTT AACGTGCACA AGACCATGGT GCAAGCTCTT 
CAGGACAAGA TACTGAAGGA GAAGTTTGGC ATGGTGGCAC CAGATGTGCA GATTGAGGAC 
GGAAAGGGAA CGATCCTCAT CTCTTCAGAG GAAGGAGAGA CTGAAGCAAA CAATAACAAG 
TTTTTGTCTG ACTTTGGGAT TCGGAATGGG AGCCGTCTTC AAGCTGACGA CTTCCTACAG 
GATTACACTC TTCTGGTCAA TGTAATTCAC AGTGAGGAGT TGGAAAAGGA TGTTGAGTTT 
GAAGTAGTCG GTGATGCCCC GGATAAAGCG CCTGCTCCAA GTGCCCCAGA AGAAGGGAAA 
AACATTGCCA ATGGGAATAA AGATTCTGCC CAGCCATCTA CCTCATCAAA AGCTGTAGAG 
GACGACGATG TCCTTCTCGT AGACTCTGAT GAAGAGCCGT CATCAAGCAC CATGGACACA 
GAGTCCAGTA ACCGCAAGAG GAAACATCAT GATGCTGAAA CAGACGATGC TTCATCTAAG 
CGCAAACGCC TGGACCAGCA GCCGGCCGAT GATGATGATG AAGACATCAT CGCTCTAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Parp inhibition prevents ten-eleven translocase enzyme activation and hyperglycemia-induced DNA demethylation.
Diabetes63(9)3069-76(2014 Sep)
Dhliwayo N,Sarras MP Jr,Luczkowski E,Mason SM,Intine RV

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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UBA2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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