NCU03526 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03526
Entrez Gene ID	3872972
Full Name	ubiquitin-activating enzyme E1 3
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395251.1	XP_011393553.1	ubiquitin-activating enzyme E1 3, variant
XM_011395250.1	XP_011393552.1	ubiquitin-activating enzyme E1 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBA2	NP_001076850.1
Rattus norvegicus (Norway rat)	Uba2	NP_001094049.1
Pan troglodytes (chimpanzee)	UBA2	XP_524210.2
Canis lupus familiaris (dog)	UBA2	XP_533699.3
Mus musculus (house mouse)	Uba2	NP_057891.1
Danio rerio (zebrafish)	uba2	NP_998528.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008637	XP_314735.3
Kluyveromyces lactis	KLLA0E03103g	XP_454085.1
Schizosaccharomyces pombe (fission yeast)	fub2	NP_595945.1
Magnaporthe oryzae (rice blast fungus)	MGG_06733	XP_003709447.1
Arabidopsis thaliana (thale cress)	SAE2	NP_001189570.1
Oryza sativa (rice)	Os07g0586500	NP_001060130.1
	UBA2	XP_001091974.1
Saccharomyces cerevisiae (baker's yeast)	UBA2	NP_010678.3
Eremothecium gossypii	AGOS_AFR138W	NP_985685.1
Neurospora crassa	NCU03526	XP_956805.2
Drosophila melanogaster (fruit fly)	Uba2	NP_524756.2
Homo sapiens (human)	UBA2	NP_005490.1
Gallus gallus (chicken)	UBA2	NP_001025742.1
Xenopus tropicalis (tropical clawed frog)	uba2	NP_001017091.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03526 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03526 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf05859	XM_011395250.1 Latest version!	Neurospora crassa OR74A ubiquitin-activating enzyme E1 3 (NCU03526), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ONf10191	XM_011395251.1 Latest version!	Neurospora crassa OR74A ubiquitin-activating enzyme E1 3, variant (NCU03526), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03526 ( XM_011395250.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393552.1 Neurospora crassa OR74A ubiquitin-activating enzyme E1 3 (NCU03526), mRNA.

Price & Availability↑

CloneID	ONf05859
Clone ID Related Accession (Same CDS sequence)	XM_011395250.1
Accession Version	XM_011395250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ubiquitin-activating enzyme E1 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGATCCCG CAATGGGCAA TCCCATGGTC CCCATGACGG CGCAAATACC AGCACCAGTT 
GCCCAGCCTA TTCCCTTGCC GGTGTTGACG CGGGACAGCT ACAATTCGCA ATCATTAGGG 
CGGTCTTTGA ATGCAAATGT CAAGCAGGCG CGCGTGCTCA TGGTAGGCGC CGGCGGCATC 
GGCTGCGAAC TCCTCAAGAA CCTCGTCCTT ACCGGCTTCG GTGAAGTCCA CGTCGTCGAT 
CTCGACACCA TCGATCTCTC CAACCTCAAC CGCCAGTTTC TCTTTCGTCA CGAACATATC 
AAAAAATCAA AGGCACTTGT AGCCAAGGAG GCTGCGCAAA AGTTCAACCC CGCCGTTAAG 
ATTGTCGCCC ACCATGCGAA CATCAAAGAT GCGCAGTTCA ATATCGAGTG GTTCAGCAGT 
TTCCGGATAG TCTTCAACGC GCTCGACAAC CTCGAAGCCC GTCGCCATGT CAACAAGATG 
TGTCTTGCCG CCGACGTCCC CCTCATCGAA AGTGGAACAA CCGGCTTCAA CGGCCAGGTA 
CAAGTCATCA AAAAGGGTGT CACAGCCTGC TACGATTGCG CGCCCAAGGA GACGCCAAAG 
TCATTCCCCG TATGCACGAT TCGCAGTACA CCGAGCCAGC CTATTCACTG TATAGTGTGG 
GGCAAGAGCT ACTTGCTCAA CGAGATTTTC GGAGCGAGCG AAGACGAGTC AGCATTCGAT 
CATACTGTTG ATGGTGATAA TGCGCAGGAG ATCGAGGAGC TCAAACGCGA GTCGGCGGCG 
CTACGAAAGA TCAGGAATTC TGTGGGTACA GAAGAGTTCG CGCAGATGCT TTTCGAAAAG 
GTATTCAAGA CGGATATCGA GCGGTTGCGC TCAATGGAGG ACATGTGGAA GACGCGCAAG 
CCGCCTGAGC CACTCAACTA CAAGGAACTC CTCGATAAGG CCAAGTCGCT CGACAAGGAC 
AAGGTTTTGA AGGATGCTCA GAAGGTGTGG TCGCTGGAAG AGAACCTCGT CGTCTTCAAT 
GACAGTCTCG AGCGACTTAG CAAGAGGGTT CTGGAGAACA AGAGCGCCGG TGAAGAATCC 
ATCATCACCT TTGACAAGGA CGATGAGGAT ACCCTGGACT TTGTCGCCGC GAGTGCCAAC 
ATTCGCTCAG CGGTGTTCGG TATTGACAGA AAGTCAAAGT TCGACATTAA GCAGATGGCT 
GGCAACATTA TCCCTGCCAT TGCGACAACG AACGCCATCG TCGCCGGTCT TTGCGTGCTG 
GAGGCATTCA AGGTCCTGAA GGGCCACTAC GAGCAGGCCA AGGAGGTTTT CCTTACGCCG 
TTTGCTAATG CACGTATGTT GGCGTCCGAC AAGTCACGGG AGCCTAACCC TGATTGCCCG 
GTCTGCGGTG TTTACCAGAC GAGAGCGTAT GTCGATTTGG AGAAGGCGAC GTTGAACGAT 
TTGGTAGAAC ACTTGATCAA GACGAATCTG GGGTATGGCG AGAAGGATTT CGCTATTAGT 
AACGAGGTTG GCATTCTGTA CGACCCGGAT GAGACGGATA ATCTGGAGAA GAAGCTTTCG 
GAGCTGGGCA TCAAATCCGA TTCATTCCTG ACCATCACCG ACGAAGACGA TGAGGAACCT 
TTCGTTAACG TCGTCGTTGC CATCCAAGAA GCCAAGGAGC CTCTGGGTGA CAAGCCAGTC 
AAGGGCATTC TCGACCCAGA GGACGTCAAG ATCCCACTCA AGCCCAAGAA GCAGTCACAG 
CCTGAGCCAG TGGCTACGCC GACAGCTGCC ACCAACGGTG CCAGCATATC CAACGGCCAG 
AACGGAGGAG TAATAAACCT TGATGGCGAT GAGCCAATGA CGACACCAGC CAAGTCGCTC 
AAGCGCGGCC ACCCCGAAGA CGCAGAGGGT CCATCGGTCA AGAAGATCAA GGCCAACGAC 
AAGGCGGCGG ATGACGATAT CGTCTTTATC GAGGATAGTG CGGGAGCTAT CGTGATTGAT 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393552.1
CDS	272..2260
Translation	MDPAMGNPMVPMTAQIPAPVAQPIPLPVLTRDSYNSQSLGRSLNANVKQARVLMVGAGGIGCELLKNLVLTGFGEVHVVDLDTIDLSNLNRQFLFRHEHIKKSKALVAKEAAQKFNPAVKIVAHHANIKDAQFNIEWFSSFRIVFNALDNLEARRHVNKMCLAADVPLIESGTTGFNGQVQVIKKGVTACYDCAPKETPKSFPVCTIRSTPSQPIHCIVWGKSYLLNEIFGASEDESAFDHTVDGDNAQEIEELKRESAALRKIRNSVGTEEFAQMLFEKVFKTDIERLRSMEDMWKTRKPPEPLNYKELLDKAKSLDKDKVLKDAQKVWSLEENLVVFNDSLERLSKRVLENKSAGEESIITFDKDDEDTLDFVAASANIRSAVFGIDRKSKFDIKQMAGNIIPAIATTNAIVAGLCVLEAFKVLKGHYEQAKEVFLTPFANARMLASDKSREPNPDCPVCGVYQTRAYVDLEKATLNDLVEHLIKTNLGYGEKDFAISNEVGILYDPDETDNLEKKLSELGIKSDSFLTITDEDDEEPFVNVVVAIQEAKEPLGDKPVKGILDPEDVKIPLKPKKQSQPEPVATPTAATNGASISNGQNGGVINLDGDEPMTTPAKSLKRGHPEDAEGPSVKKIKANDKAADDDIVFIEDSAGAIVIDDD

Target ORF information:

RefSeq Version	XM_011395250.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ubiquitin-activating enzyme E1 3 (NCU03526), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395250.1

ORF Insert Sequence:

ATGGATCCCG CAATGGGCAA TCCCATGGTC CCCATGACGG CGCAAATACC AGCACCAGTT 
GCCCAGCCTA TTCCCTTGCC GGTGTTGACG CGGGACAGCT ACAATTCGCA ATCATTAGGG 
CGGTCTTTGA ATGCAAATGT CAAGCAGGCG CGCGTGCTCA TGGTAGGCGC CGGCGGCATC 
GGCTGCGAAC TCCTCAAGAA CCTCGTCCTT ACCGGCTTCG GTGAAGTCCA CGTCGTCGAT 
CTCGACACCA TCGATCTCTC CAACCTCAAC CGCCAGTTTC TCTTTCGTCA CGAACATATC 
AAAAAATCAA AGGCACTTGT AGCCAAGGAG GCTGCGCAAA AGTTCAACCC CGCCGTTAAG 
ATTGTCGCCC ACCATGCGAA CATCAAAGAT GCGCAGTTCA ATATCGAGTG GTTCAGCAGT 
TTCCGGATAG TCTTCAACGC GCTCGACAAC CTCGAAGCCC GTCGCCATGT CAACAAGATG 
TGTCTTGCCG CCGACGTCCC CCTCATCGAA AGTGGAACAA CCGGCTTCAA CGGCCAGGTA 
CAAGTCATCA AAAAGGGTGT CACAGCCTGC TACGATTGCG CGCCCAAGGA GACGCCAAAG 
TCATTCCCCG TATGCACGAT TCGCAGTACA CCGAGCCAGC CTATTCACTG TATAGTGTGG 
GGCAAGAGCT ACTTGCTCAA CGAGATTTTC GGAGCGAGCG AAGACGAGTC AGCATTCGAT 
CATACTGTTG ATGGTGATAA TGCGCAGGAG ATCGAGGAGC TCAAACGCGA GTCGGCGGCG 
CTACGAAAGA TCAGGAATTC TGTGGGTACA GAAGAGTTCG CGCAGATGCT TTTCGAAAAG 
GTATTCAAGA CGGATATCGA GCGGTTGCGC TCAATGGAGG ACATGTGGAA GACGCGCAAG 
CCGCCTGAGC CACTCAACTA CAAGGAACTC CTCGATAAGG CCAAGTCGCT CGACAAGGAC 
AAGGTTTTGA AGGATGCTCA GAAGGTGTGG TCGCTGGAAG AGAACCTCGT CGTCTTCAAT 
GACAGTCTCG AGCGACTTAG CAAGAGGGTT CTGGAGAACA AGAGCGCCGG TGAAGAATCC 
ATCATCACCT TTGACAAGGA CGATGAGGAT ACCCTGGACT TTGTCGCCGC GAGTGCCAAC 
ATTCGCTCAG CGGTGTTCGG TATTGACAGA AAGTCAAAGT TCGACATTAA GCAGATGGCT 
GGCAACATTA TCCCTGCCAT TGCGACAACG AACGCCATCG TCGCCGGTCT TTGCGTGCTG 
GAGGCATTCA AGGTCCTGAA GGGCCACTAC GAGCAGGCCA AGGAGGTTTT CCTTACGCCG 
TTTGCTAATG CACGTATGTT GGCGTCCGAC AAGTCACGGG AGCCTAACCC TGATTGCCCG 
GTCTGCGGTG TTTACCAGAC GAGAGCGTAT GTCGATTTGG AGAAGGCGAC GTTGAACGAT 
TTGGTAGAAC ACTTGATCAA GACGAATCTG GGGTATGGCG AGAAGGATTT CGCTATTAGT 
AACGAGGTTG GCATTCTGTA CGACCCGGAT GAGACGGATA ATCTGGAGAA GAAGCTTTCG 
GAGCTGGGCA TCAAATCCGA TTCATTCCTG ACCATCACCG ACGAAGACGA TGAGGAACCT 
TTCGTTAACG TCGTCGTTGC CATCCAAGAA GCCAAGGAGC CTCTGGGTGA CAAGCCAGTC 
AAGGGCATTC TCGACCCAGA GGACGTCAAG ATCCCACTCA AGCCCAAGAA GCAGTCACAG 
CCTGAGCCAG TGGCTACGCC GACAGCTGCC ACCAACGGTG CCAGCATATC CAACGGCCAG 
AACGGAGGAG TAATAAACCT TGATGGCGAT GAGCCAATGA CGACACCAGC CAAGTCGCTC 
AAGCGCGGCC ACCCCGAAGA CGCAGAGGGT CCATCGGTCA AGAAGATCAA GGCCAACGAC 
AAGGCGGCGG ATGACGATAT CGTCTTTATC GAGGATAGTG CGGGAGCTAT CGTGATTGAT 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03526 ( XM_011395251.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393553.1 Neurospora crassa OR74A ubiquitin-activating enzyme E1 3, variant (NCU03526), mRNA.

Price & Availability↑

CloneID	ONf10191
Clone ID Related Accession (Same CDS sequence)	XM_011395251.1
Accession Version	XM_011395251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ubiquitin-activating enzyme E1 3, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGTAGGCG CCGGCGGCAT CGGCTGCGAA CTCCTCAAGA ACCTCGTCCT TACCGGCTTC 
GGTGAAGTCC ACGTCGTCGA TCTCGACACC ATCGATCTCT CCAACCTCAA CCGCCAGTTT 
CTCTTTCGTC ACGAACATAT CAAAAAATCA AAGGCACTTG TAGCCAAGGA GGCTGCGCAA 
AAGTTCAACC CCGCCGTTAA GATTGTCGCC CACCATGCGA ACATCAAAGA TGCGCAGTTC 
AATATCGAGT GGTTCAGCAG TTTCCGGATA GTCTTCAACG CGCTCGACAA CCTCGAAGCC 
CGTCGCCATG TCAACAAGAT GTGTCTTGCC GCCGACGTCC CCCTCATCGA AAGTGGAACA 
ACCGGCTTCA ACGGCCAGGT ACAAGTCATC AAAAAGGGTG TCACAGCCTG CTACGATTGC 
GCGCCCAAGG AGACGCCAAA GTCATTCCCC GTATGCACGA TTCGCAGTAC ACCGAGCCAG 
CCTATTCACT GTATAGTGTG GGGCAAGAGC TACTTGCTCA ACGAGATTTT CGGAGCGAGC 
GAAGACGAGT CAGCATTCGA TCATACTGTT GATGGTGATA ATGCGCAGGA GATCGAGGAG 
CTCAAACGCG AGTCGGCGGC GCTACGAAAG ATCAGGAATT CTGTGGGTAC AGAAGAGTTC 
GCGCAGATGC TTTTCGAAAA GGTATTCAAG ACGGATATCG AGCGGTTGCG CTCAATGGAG 
GACATGTGGA AGACGCGCAA GCCGCCTGAG CCACTCAACT ACAAGGAACT CCTCGATAAG 
GCCAAGTCGC TCGACAAGGA CAAGGTTTTG AAGGATGCTC AGAAGGTGTG GTCGCTGGAA 
GAGAACCTCG TCGTCTTCAA TGACAGTCTC GAGCGACTTA GCAAGAGGGT TCTGGAGAAC 
AAGAGCGCCG GTGAAGAATC CATCATCACC TTTGACAAGG ACGATGAGGA TACCCTGGAC 
TTTGTCGCCG CGAGTGCCAA CATTCGCTCA GCGGTGTTCG GTATTGACAG AAAGTCAAAG 
TTCGACATTA AGCAGATGGC TGGCAACATT ATCCCTGCCA TTGCGACAAC GAACGCCATC 
GTCGCCGGTC TTTGCGTGCT GGAGGCATTC AAGGTCCTGA AGGGCCACTA CGAGCAGGCC 
AAGGAGGTTT TCCTTACGCC GTTTGCTAAT GCACGTATGT TGGCGTCCGA CAAGTCACGG 
GAGCCTAACC CTGATTGCCC GGTCTGCGGT GTTTACCAGA CGAGAGCGTA TGTCGATTTG 
GAGAAGGCGA CGTTGAACGA TTTGGTAGAA CACTTGATCA AGACGAATCT GGGGTATGGC 
GAGAAGGATT TCGCTATTAG TAACGAGGTT GGCATTCTGT ACGACCCGGA TGAGACGGAT 
AATCTGGAGA AGAAGCTTTC GGAGCTGGGC ATCAAATCCG ATTCATTCCT GACCATCACC 
GACGAAGACG ATGAGGAACC TTTCGTTAAC GTCGTCGTTG CCATCCAAGA AGCCAAGGAG 
CCTCTGGGTG ACAAGCCAGT CAAGGGCATT CTCGACCCAG AGGACGTCAA GATCCCACTC 
AAGCCCAAGA AGCAGTCACA GCCTGAGCCA GTGGCTACGC CGACAGCTGC CACCAACGGT 
GCCAGCATAT CCAACGGCCA GAACGGAGGA GTAATAAACC TTGATGGCGA TGAGCCAATG 
ACGACACCAG CCAAGTCGCT CAAGCGCGGC CACCCCGAAG ACGCAGAGGG TCCATCGGTC 
AAGAAGATCA AGGCCAACGA CAAGGCGGCG GATGACGATA TCGTCTTTAT CGAGGATAGT 
GCGGGAGCTA TCGTGATTGA TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393553.1
CDS	490..2319
Translation	MVGAGGIGCELLKNLVLTGFGEVHVVDLDTIDLSNLNRQFLFRHEHIKKSKALVAKEAAQKFNPAVKIVAHHANIKDAQFNIEWFSSFRIVFNALDNLEARRHVNKMCLAADVPLIESGTTGFNGQVQVIKKGVTACYDCAPKETPKSFPVCTIRSTPSQPIHCIVWGKSYLLNEIFGASEDESAFDHTVDGDNAQEIEELKRESAALRKIRNSVGTEEFAQMLFEKVFKTDIERLRSMEDMWKTRKPPEPLNYKELLDKAKSLDKDKVLKDAQKVWSLEENLVVFNDSLERLSKRVLENKSAGEESIITFDKDDEDTLDFVAASANIRSAVFGIDRKSKFDIKQMAGNIIPAIATTNAIVAGLCVLEAFKVLKGHYEQAKEVFLTPFANARMLASDKSREPNPDCPVCGVYQTRAYVDLEKATLNDLVEHLIKTNLGYGEKDFAISNEVGILYDPDETDNLEKKLSELGIKSDSFLTITDEDDEEPFVNVVVAIQEAKEPLGDKPVKGILDPEDVKIPLKPKKQSQPEPVATPTAATNGASISNGQNGGVINLDGDEPMTTPAKSLKRGHPEDAEGPSVKKIKANDKAADDDIVFIEDSAGAIVIDDD

Target ORF information:

RefSeq Version	XM_011395251.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ubiquitin-activating enzyme E1 3, variant (NCU03526), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395251.1

ORF Insert Sequence:

ATGGTAGGCG CCGGCGGCAT CGGCTGCGAA CTCCTCAAGA ACCTCGTCCT TACCGGCTTC 
GGTGAAGTCC ACGTCGTCGA TCTCGACACC ATCGATCTCT CCAACCTCAA CCGCCAGTTT 
CTCTTTCGTC ACGAACATAT CAAAAAATCA AAGGCACTTG TAGCCAAGGA GGCTGCGCAA 
AAGTTCAACC CCGCCGTTAA GATTGTCGCC CACCATGCGA ACATCAAAGA TGCGCAGTTC 
AATATCGAGT GGTTCAGCAG TTTCCGGATA GTCTTCAACG CGCTCGACAA CCTCGAAGCC 
CGTCGCCATG TCAACAAGAT GTGTCTTGCC GCCGACGTCC CCCTCATCGA AAGTGGAACA 
ACCGGCTTCA ACGGCCAGGT ACAAGTCATC AAAAAGGGTG TCACAGCCTG CTACGATTGC 
GCGCCCAAGG AGACGCCAAA GTCATTCCCC GTATGCACGA TTCGCAGTAC ACCGAGCCAG 
CCTATTCACT GTATAGTGTG GGGCAAGAGC TACTTGCTCA ACGAGATTTT CGGAGCGAGC 
GAAGACGAGT CAGCATTCGA TCATACTGTT GATGGTGATA ATGCGCAGGA GATCGAGGAG 
CTCAAACGCG AGTCGGCGGC GCTACGAAAG ATCAGGAATT CTGTGGGTAC AGAAGAGTTC 
GCGCAGATGC TTTTCGAAAA GGTATTCAAG ACGGATATCG AGCGGTTGCG CTCAATGGAG 
GACATGTGGA AGACGCGCAA GCCGCCTGAG CCACTCAACT ACAAGGAACT CCTCGATAAG 
GCCAAGTCGC TCGACAAGGA CAAGGTTTTG AAGGATGCTC AGAAGGTGTG GTCGCTGGAA 
GAGAACCTCG TCGTCTTCAA TGACAGTCTC GAGCGACTTA GCAAGAGGGT TCTGGAGAAC 
AAGAGCGCCG GTGAAGAATC CATCATCACC TTTGACAAGG ACGATGAGGA TACCCTGGAC 
TTTGTCGCCG CGAGTGCCAA CATTCGCTCA GCGGTGTTCG GTATTGACAG AAAGTCAAAG 
TTCGACATTA AGCAGATGGC TGGCAACATT ATCCCTGCCA TTGCGACAAC GAACGCCATC 
GTCGCCGGTC TTTGCGTGCT GGAGGCATTC AAGGTCCTGA AGGGCCACTA CGAGCAGGCC 
AAGGAGGTTT TCCTTACGCC GTTTGCTAAT GCACGTATGT TGGCGTCCGA CAAGTCACGG 
GAGCCTAACC CTGATTGCCC GGTCTGCGGT GTTTACCAGA CGAGAGCGTA TGTCGATTTG 
GAGAAGGCGA CGTTGAACGA TTTGGTAGAA CACTTGATCA AGACGAATCT GGGGTATGGC 
GAGAAGGATT TCGCTATTAG TAACGAGGTT GGCATTCTGT ACGACCCGGA TGAGACGGAT 
AATCTGGAGA AGAAGCTTTC GGAGCTGGGC ATCAAATCCG ATTCATTCCT GACCATCACC 
GACGAAGACG ATGAGGAACC TTTCGTTAAC GTCGTCGTTG CCATCCAAGA AGCCAAGGAG 
CCTCTGGGTG ACAAGCCAGT CAAGGGCATT CTCGACCCAG AGGACGTCAA GATCCCACTC 
AAGCCCAAGA AGCAGTCACA GCCTGAGCCA GTGGCTACGC CGACAGCTGC CACCAACGGT 
GCCAGCATAT CCAACGGCCA GAACGGAGGA GTAATAAACC TTGATGGCGA TGAGCCAATG 
ACGACACCAG CCAAGTCGCT CAAGCGCGGC CACCCCGAAG ACGCAGAGGG TCCATCGGTC 
AAGAAGATCA AGGCCAACGA CAAGGCGGCG GATGACGATA TCGTCTTTAT CGAGGATAGT 
GCGGGAGCTA TCGTGATTGA TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03526 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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