Uba2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uba2
Entrez Gene ID	44496
Full Name	Ubiquitin activating enzyme 2
Synonyms	CG7528,DmSAE2,DmSae2,DmUba2,Dmel\CG7528,Dmuba2,L36,SAE2,Sae2,UBA2,Ubc2,dUba2,sip2,uba2
General protein information	Preferred Names Ubiquitin activating enzyme 2 Names CG7528 gene product from transcript CG7528-RA CG7528-PA Smt3 activating enzyme 2 Smt3-activating enzyme 2 Uba2-PA ubiquitin activating enzyme 2 ubiquitin-activating enzyme (E1)
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66B7-66B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_080017.3	NP_524756.2	ubiquitin activating enzyme 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBA2	NP_001076850.1
Rattus norvegicus (Norway rat)	Uba2	NP_001094049.1
Pan troglodytes (chimpanzee)	UBA2	XP_524210.2
Canis lupus familiaris (dog)	UBA2	XP_533699.3
Mus musculus (house mouse)	Uba2	NP_057891.1
Danio rerio (zebrafish)	uba2	NP_998528.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008637	XP_314735.3
Kluyveromyces lactis	KLLA0E03103g	XP_454085.1
Schizosaccharomyces pombe (fission yeast)	fub2	NP_595945.1
Magnaporthe oryzae (rice blast fungus)	MGG_06733	XP_003709447.1
Arabidopsis thaliana (thale cress)	SAE2	NP_001189570.1
Oryza sativa (rice)	Os07g0586500	NP_001060130.1
	UBA2	XP_001091974.1
Saccharomyces cerevisiae (baker's yeast)	UBA2	NP_010678.3
Eremothecium gossypii	AGOS_AFR138W	NP_985685.1
Neurospora crassa	NCU03526	XP_956805.2
Drosophila melanogaster (fruit fly)	Uba2	NP_524756.2
Homo sapiens (human)	UBA2	NP_005490.1
Gallus gallus (chicken)	UBA2	NP_001025742.1
Xenopus tropicalis (tropical clawed frog)	uba2	NP_001017091.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knockdown of SUMO or its E1 and E2 enzymes robustly disrupts proliferating cells in the developing eye, without any detectable effects on the development or differentiation of neighboring post-mitotic cells
Title: A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development.

The following Uba2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uba2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20480	NM_080017.3 Latest version!	Drosophila melanogaster ubiquitin activating enzyme 2 (Uba2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uba2 ( NM_080017.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524756.2 Drosophila melanogaster ubiquitin activating enzyme 2 (Uba2), mRNA.

Price & Availability↑

CloneID	OFa20480
Clone ID Related Accession (Same CDS sequence)	NM_080017.3
Accession Version	NM_080017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin activating enzyme 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080017.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAG CTATCAATGG TGTTTTCCCA CCCACATTGC AGGAGCTGGT AAAGAAGTCC 
AAGGTGCTGG TCGTGGGCGC CGGCGGAATC GGCTGCGAGG TGCTCAAAAA CCTTGTGCTT 
AGCGGCTTTA CAGACATCGA AATTATCGAT CTGGACACAA TTGATCTTAG CAACTTGAAC 
CGACAGTTCC TATTCCATCG CGAGCACGTT GGAAAATCTA AGGCACGGGT GGCTAGGGAA 
AGCGCACTGA GCTTCAATCC GGACGCTAAG ATAACCGCTT ACCACGACAG TGTAACATCT 
ACTGATTATG GCGTCAACTT CTTTAAGAAG TTCGATTTGG TGCTCAGCGC CTTGGACAAC 
AGGGCCGCCA GGAATCATGT AAATCGCATG TGCCTCAATG CGGATGTCCC GCTGATCGAG 
AGCGGCACCG CCGGCTACAA TGGCCAGGTG GAACTGATCA AGCGTGGACT CACCCAGTGC 
TACGAGTGCA CTCCAAAAGA CAAACAGCGA AGCTTCCCGG GCTGCACCAT ACGCAATACG 
CCCTCCGAAC CGATTCACTG CATTGTCTGG GCCAAGCATC TCTTTAATCA ACTGTTTGGT 
GAGTCCTTGG AAGATGAGGA TATTTCTCCT GATGCTGCTG ATCCCGATGC AAAAGAAAAA 
GACGGCGGCG ATGGAAATGG CGAGCCCAAA GGCGATGGGA AGGAAAAAGG CGAAGAGTCA 
AAAGAGGAAA AGGAGGCCAA GGAGGATACT GCCAACGGCA ACATAATGCG CATTAATACT 
CGCCAATGGG CCAAGGACTG CAACTATGAT GCGGGCAAGC TGTTCAACAA GTTCTTTAAC 
GAGGACATTA CCTATTTGTT GCGTATGTCG AATTTGTGGA AGACCCGCAA GGCACCCGTG 
CCCGTGCAGT GGGATACCCT GCTGCCCGAA GGATCCTCCG GTGATCAGAA GGATGTGGCC 
AAGCAGCATC ACAAGGTGTG GTCCATCGAG GAGTGCGCTC AGGTCTTTGC CAATTCATTA 
AAAGAGTTGA GCGCTAACTT CCTGAAACTC GAAGGCGACG ATACTCTGGC TTGGGACAAG 
GACGACCAGC CAGCCATGGA TTTCGTGGCG GCCTGCGCCA ACGTGCGATC CCACATTTTC 
GACATTGAGC GAAAGTCAAG GTTCGAGATT AAGTCAATGG CGGGAAACAT TATTCCTGCT 
ATTGCCACCA CAAATGCGAT TACGGCGGGA ATTTCCGTGA TGCGGGCTTT CAAAGTGCTG 
GAGGCCAAGT GGGAGCAGTG CAAGGCCGTC TATGCCCGAC TTAGACCAAA TGCACGAAAT 
CACTTCCTTG TACCGGACGC TTCTCTTCCT GGCCCCAATC CCAATTGCCA TGTATGCGCC 
AGCGATCCGG CCATTACTCT CAAGATCGAT ACGAAGCGCA TGCGTATAAA GGAGCTGCGT 
GACGAGGTCC TGGTTAAGAC GCTCAACATG TTGAATCCGG ATGTGACTGT GCAGAGCAAT 
GGCTCCATTT TAATCTCCTC AGAGGAGGGC GAGACCGAAT GCAATGACGG CAAGCTCCTA 
AGCGAATTAA ACATTGTGGA TGGTGTGATC CTCAAGTGCG ACGACTTCTT CCAGAACTAC 
GAGTTGAGTA TTATCATTTC CCACTTCGAT GCGGAGCGCG ATGAAAACTT GTTCGAAGTC 
GTAGCAGATG CCTCACAGCT GAAGCCAAAG GATGAGGATC AGAAGGAGGC CGTGAAAGAC 
AAGGAGGATG AACCGAAATC GGCTAAGAAG CGTTCTACCA ACGGGGAAGG AGACTCAAAG 
GACGATGGAC CCTCCACTTC GAAGCGCAGC CGGCCCAACG AAGTGGTTGA GGAAGATGAC 
GATGACTGTC TGGTAATCGA GGAAGACGAA GACCAAGCAG ATGTTGTTGT CGTGGCCACA 
GACAAGCTCT CTGTGCAGAG TCCCCCAAAA TCGGGCTCCA AGCGCAAGCC ATGTGAAGTA 
ATCGAGGATG AGGATATCAC CGAGATTTTG GAGTCGTCTG ACGACGAACC CGCGGGACCA 
ACCAAGTGCA AACGTTCCCG CCTGGACGAT TCGAACCCCG TGGCAGTCAT CAGTATCGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524756.2
CDS	115..2217
Translation	MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQVELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFGESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDTANGNIMRINTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPVPVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKLEGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPAIATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLPGPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSNGSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFDAERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSKDDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPKSGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID

Target ORF information:

RefSeq Version	NM_080017.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin activating enzyme 2 (Uba2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080017.3

ORF Insert Sequence:

ATGGCAGCAG CTATCAATGG TGTTTTCCCA CCCACATTGC AGGAGCTGGT AAAGAAGTCC 
AAGGTGCTGG TCGTGGGCGC CGGCGGAATC GGCTGCGAGG TGCTCAAAAA CCTTGTGCTT 
AGCGGCTTTA CAGACATCGA AATTATCGAT CTGGACACAA TTGATCTTAG CAACTTGAAC 
CGACAGTTCC TATTCCATCG CGAGCACGTT GGAAAATCTA AGGCACGGGT GGCTAGGGAA 
AGCGCACTGA GCTTCAATCC GGACGCTAAG ATAACCGCTT ACCACGACAG TGTAACATCT 
ACTGATTATG GCGTCAACTT CTTTAAGAAG TTCGATTTGG TGCTCAGCGC CTTGGACAAC 
AGGGCCGCCA GGAATCATGT AAATCGCATG TGCCTCAATG CGGATGTCCC GCTGATCGAG 
AGCGGCACCG CCGGCTACAA TGGCCAGGTG GAACTGATCA AGCGTGGACT CACCCAGTGC 
TACGAGTGCA CTCCAAAAGA CAAACAGCGA AGCTTCCCGG GCTGCACCAT ACGCAATACG 
CCCTCCGAAC CGATTCACTG CATTGTCTGG GCCAAGCATC TCTTTAATCA ACTGTTTGGT 
GAGTCCTTGG AAGATGAGGA TATTTCTCCT GATGCTGCTG ATCCCGATGC AAAAGAAAAA 
GACGGCGGCG ATGGAAATGG CGAGCCCAAA GGCGATGGGA AGGAAAAAGG CGAAGAGTCA 
AAAGAGGAAA AGGAGGCCAA GGAGGATACT GCCAACGGCA ACATAATGCG CATTAATACT 
CGCCAATGGG CCAAGGACTG CAACTATGAT GCGGGCAAGC TGTTCAACAA GTTCTTTAAC 
GAGGACATTA CCTATTTGTT GCGTATGTCG AATTTGTGGA AGACCCGCAA GGCACCCGTG 
CCCGTGCAGT GGGATACCCT GCTGCCCGAA GGATCCTCCG GTGATCAGAA GGATGTGGCC 
AAGCAGCATC ACAAGGTGTG GTCCATCGAG GAGTGCGCTC AGGTCTTTGC CAATTCATTA 
AAAGAGTTGA GCGCTAACTT CCTGAAACTC GAAGGCGACG ATACTCTGGC TTGGGACAAG 
GACGACCAGC CAGCCATGGA TTTCGTGGCG GCCTGCGCCA ACGTGCGATC CCACATTTTC 
GACATTGAGC GAAAGTCAAG GTTCGAGATT AAGTCAATGG CGGGAAACAT TATTCCTGCT 
ATTGCCACCA CAAATGCGAT TACGGCGGGA ATTTCCGTGA TGCGGGCTTT CAAAGTGCTG 
GAGGCCAAGT GGGAGCAGTG CAAGGCCGTC TATGCCCGAC TTAGACCAAA TGCACGAAAT 
CACTTCCTTG TACCGGACGC TTCTCTTCCT GGCCCCAATC CCAATTGCCA TGTATGCGCC 
AGCGATCCGG CCATTACTCT CAAGATCGAT ACGAAGCGCA TGCGTATAAA GGAGCTGCGT 
GACGAGGTCC TGGTTAAGAC GCTCAACATG TTGAATCCGG ATGTGACTGT GCAGAGCAAT 
GGCTCCATTT TAATCTCCTC AGAGGAGGGC GAGACCGAAT GCAATGACGG CAAGCTCCTA 
AGCGAATTAA ACATTGTGGA TGGTGTGATC CTCAAGTGCG ACGACTTCTT CCAGAACTAC 
GAGTTGAGTA TTATCATTTC CCACTTCGAT GCGGAGCGCG ATGAAAACTT GTTCGAAGTC 
GTAGCAGATG CCTCACAGCT GAAGCCAAAG GATGAGGATC AGAAGGAGGC CGTGAAAGAC 
AAGGAGGATG AACCGAAATC GGCTAAGAAG CGTTCTACCA ACGGGGAAGG AGACTCAAAG 
GACGATGGAC CCTCCACTTC GAAGCGCAGC CGGCCCAACG AAGTGGTTGA GGAAGATGAC 
GATGACTGTC TGGTAATCGA GGAAGACGAA GACCAAGCAG ATGTTGTTGT CGTGGCCACA 
GACAAGCTCT CTGTGCAGAG TCCCCCAAAA TCGGGCTCCA AGCGCAAGCC ATGTGAAGTA 
ATCGAGGATG AGGATATCAC CGAGATTTTG GAGTCGTCTG ACGACGAACC CGCGGGACCA 
ACCAAGTGCA AACGTTCCCG CCTGGACGAT TCGAACCCCG TGGCAGTCAT CAGTATCGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Uba2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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