Ankfn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankfn1
Entrez Gene ID	382543
Full Name	ankyrin-repeat and fibronectin type III domain containing 1
Synonyms	4932411E22Rik,Gm1175,nmf9
General protein information	Preferred Names ankyrin-repeat and fibronectin type III domain containing 1 Names ankyrin repeat and fibronectin type-III domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314636.1	XP_017170125.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_030246105.1	XP_030101965.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_017314636.2	XP_017170125.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_030246107.1	XP_030101967.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
NM_001327810.1	NP_001314739.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 1
NM_172534.2	NP_766122.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 2
XM_030246106.1	XP_030101966.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANKFN1	XP_511898.4
Danio rerio (zebrafish)	LOC100333998	XP_003201826.2
Danio rerio (zebrafish)	LOC557615	XP_003199674.2
Drosophila melanogaster (fruit fly)	CG6954	NP_001247244.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001726	XP_321358.5
	LOC707235	XP_002808169.1
Homo sapiens (human)	ANKFN1	NP_694960.2
Canis lupus familiaris (dog)	ANKFN1	XP_548219.3
Bos taurus (cattle)	ANKFN1	XP_003587422.2
Mus musculus (house mouse)	Ankfn1	NP_001074402.1
Gallus gallus (chicken)	ANKFN1	XP_004946219.1
Xenopus tropicalis (tropical clawed frog)	LOC100490095	XP_004918819.1

Gene Ontology
Bibliography

Related articles in PubMed

Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies.
Zhang S, Ross KD, Seidner GA, Gorman MR, Poon TH, Wang X, Keithley EM, Lee PN, Martindale MQ, Joiner WJ, Hamilton BA
PLoS genetics11(7)e1005344(2015 Jul)

Mutation rate and predicted phenotypic target sizes in ethylnitrosourea-treated mice.
Concepcion D, Seburn KL, Wen G, Frankel WN, Hamilton BA
Genetics168(2)953-9(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ENU-induced and genome-edited null mutations in mice produce deficits in vestibular function, fear learning and circadian behavior, which correlated with Nmf9 expression in inner ear, amygdala, and suprachiasmatic nuclei. [Mnf9]
Title: Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies.

The following Ankfn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankfn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03631	NM_172534.2 Latest version!	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu76909	NM_001327810.1 Latest version!	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu77962	XM_030246105.1 Latest version!	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu77962	XM_030246106.1 Latest version!	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu77962	XM_017314636.2 Latest version!	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu77962	XM_030246107.1 Latest version!	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu77962	XM_017314636.1	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankfn1 ( NM_172534.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766122.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03631
Clone ID Related Accession (Same CDS sequence)	NM_172534.2
Accession Version	NM_172534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029964.1. On Apr 5, 2007 this sequence version replaced NM_172534.1. ##Evidence-Data-START## Transcript exon combination :: AK029964.1, BC140961.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849384, SAMN01164131 [ECO:0000350] ##Evidence-Data-END##

ATGCCGCTCT CTGGGCCTCT CCACACTGCA GCTGTTCATT TTTGCAGCTA CAGAGAGAAA 
TTTATTAGTC TGTATTGCCG CCTTTCTGCT GTTGTGGAGC TGGACTCTCT GAATACCCAA 
CAGTCCCTGA GGGAGGCAAT CTCAGACAGC GAGGTCGCAG CTGCCAAACA ACGGCACCAG 
CAAGTGCTGG ACTTTATTCA GCAAATTGAT GAAGTCTGGC GTGAGATGAG ATGGATCATG 
GATGCTCTAC AGTATGCCCG GTACAAGCAA CCAGTCTCTG GCTTGCCCAT CACTAAGCTG 
ATAGACTCCT CCAGTGAGCA GAATCTAAAG AAGATCAGTT CTACGTCATC ACACATAGAC 
TGTCTTCCGT CAACATCCCC ATCGCCAGAG ATGCACAGAA GAAAGGCAGT GAGTGAGTCA 
CAGCCCTGCT CGGATGAAGA AGGCTGCTCG GAAGTCTTCC TCCCCACAGA CAGTGACTAT 
GACTCCAGCG ATGCCCTGAG CCCACGAGAC CTGGACCTGG TTTACCTGTC ATCACATGAC 
ATAGCCCAGC AGGCCCTCAG CGGCCTCAGT GGCAGCGCCC CAGATGTCCT ACAGGTGCAT 
GACATGAAGG CCTCCATGGG ACCTGGCCAG GACCCTCAGG GCCCAGGGCA GGGTCCTGAC 
ACCGACCACT CATGTGTCGA GTTTCTCCAC AGCCTGACCC TCACGGGCTT GGCACCCAAG 
AACCATGCCA AGATGGTGGC AGGCACACGG CCGCCACTGG GCTTCTTGGG AAAGCGGAAG 
CCAGGCAAGC ACCAGCACTA TGGTGGCTTC AGCCGCCACC ACCGCTGGCT GCGCATGCAC 
AGCGAGACGC AGTCCCTGTC ACTCTCCGAA GGTGTTTACA CCCAGCACCT GTCTCAGGCC 
TGCGGCCTGG CTGAAGATCC TGGGGAGGCA GAGGGGCCTG GACCTGTGGT GGATGGGCCC 
CGAGGCCTGC CTCTGGCCCA TGCAGCCAGC CTCCCAGAGG AGAGGAGGAG CTGCCTCCAA 
GACCCAAGGC GGCCCCTGCA GAGAGTCTAT GTGGAACCCT ATTCCGACAC ACTCGCAGGC 
CAGGATCCAA AGCTGTGGAC CAGTTTGAGC CCTACCCCTG CAGGCCGCAG CAGCCTCCCA 
AGCTCCACTA GTTCAGAGAT GAGTCCAGAC CCCACATCTC CAGTTTCAGA AATATTAAGT 
AGTATGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766122.1
CDS	69..1280
Translation	MPLSGPLHTAAVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	NM_172534.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172534.2

ORF Insert Sequence:

ATGCCGCTCT CTGGGCCTCT CCACACTGCA GCTGTTCATT TTTGCAGCTA CAGAGAGAAA 
TTTATTAGTC TGTATTGCCG CCTTTCTGCT GTTGTGGAGC TGGACTCTCT GAATACCCAA 
CAGTCCCTGA GGGAGGCAAT CTCAGACAGC GAGGTCGCAG CTGCCAAACA ACGGCACCAG 
CAAGTGCTGG ACTTTATTCA GCAAATTGAT GAAGTCTGGC GTGAGATGAG ATGGATCATG 
GATGCTCTAC AGTATGCCCG GTACAAGCAA CCAGTCTCTG GCTTGCCCAT CACTAAGCTG 
ATAGACTCCT CCAGTGAGCA GAATCTAAAG AAGATCAGTT CTACGTCATC ACACATAGAC 
TGTCTTCCGT CAACATCCCC ATCGCCAGAG ATGCACAGAA GAAAGGCAGT GAGTGAGTCA 
CAGCCCTGCT CGGATGAAGA AGGCTGCTCG GAAGTCTTCC TCCCCACAGA CAGTGACTAT 
GACTCCAGCG ATGCCCTGAG CCCACGAGAC CTGGACCTGG TTTACCTGTC ATCACATGAC 
ATAGCCCAGC AGGCCCTCAG CGGCCTCAGT GGCAGCGCCC CAGATGTCCT ACAGGTGCAT 
GACATGAAGG CCTCCATGGG ACCTGGCCAG GACCCTCAGG GCCCAGGGCA GGGTCCTGAC 
ACCGACCACT CATGTGTCGA GTTTCTCCAC AGCCTGACCC TCACGGGCTT GGCACCCAAG 
AACCATGCCA AGATGGTGGC AGGCACACGG CCGCCACTGG GCTTCTTGGG AAAGCGGAAG 
CCAGGCAAGC ACCAGCACTA TGGTGGCTTC AGCCGCCACC ACCGCTGGCT GCGCATGCAC 
AGCGAGACGC AGTCCCTGTC ACTCTCCGAA GGTGTTTACA CCCAGCACCT GTCTCAGGCC 
TGCGGCCTGG CTGAAGATCC TGGGGAGGCA GAGGGGCCTG GACCTGTGGT GGATGGGCCC 
CGAGGCCTGC CTCTGGCCCA TGCAGCCAGC CTCCCAGAGG AGAGGAGGAG CTGCCTCCAA 
GACCCAAGGC GGCCCCTGCA GAGAGTCTAT GTGGAACCCT ATTCCGACAC ACTCGCAGGC 
CAGGATCCAA AGCTGTGGAC CAGTTTGAGC CCTACCCCTG CAGGCCGCAG CAGCCTCCCA 
AGCTCCACTA GTTCAGAGAT GAGTCCAGAC CCCACATCTC CAGTTTCAGA AATATTAAGT 
AGTATGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfn1 ( NM_001327810.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001314739.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu76909
Clone ID Related Accession (Same CDS sequence)	NM_001327810.1
Accession Version	NM_001327810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645903.8, AL672280.10, AL662930.29, AL772375.5 and AL672175.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTGTCCTG TCATGGAGAC ATCACCAAAC AGGAGGCTGC CCTTCAAAGA CAGACATTTT 
ACTTGCAGCA AAATAATAGG AAGGAGGTTC GCTTGCTTTG CGCAGAGGCT GAGCTACAGG 
AGAAAGCAGA GCCAATGTGA TTTATTGAAT GAAAGCACTG GACACTTACC AGCAACTTGT 
TCCTCTGCTG CCTCGAAGAG CTTAAGCTGG AACTACTGCT CGAAAATGAC CCAGCAAATG 
CAGAACTTAC ATCTTTCCCA GTCAAAAAAA CACAGTGCTC CATCGTCTCC CAATGCTGCC 
AAGCGACTGT ACAGGAACCT CTCGGAGAAG CTGAAGGGCA GTCACTCGTC CTTTGATGAG 
GCCTATTTTA GAACAAGAAC GGATCGGCTG AGTCTCAGAA AGACTTCTGT GAACTTCCAG 
GGCAACGAAG CCATGTTTGA GGCAGTTGAG CAACAGGACC TAGATGCTGT GCAACTCCTC 
CTCTACCAAT ATACACCGGA AGAACTAGAT CTGAACACAC CGAACAGCGA AGGGCTGACA 
CCCCTGGACA TCGCCATCAT GACCAACAAC GTGCCCATTG CGAGGATTCT TCTGCGGACA 
GGAGCCAGAG AAAGTCCGCA CTTTGTCAGC CTGGAGAGCC GAGCAATGCA TCTCAACACT 
CTAGTTCAGG AGGCCCAGGA CAGGGTGAGT GAGCTGTCTG CACAGGTGGA GAATGAAGGC 
TTCACCCTGG ACAACACAGA GAAAGAGAAG CAGCTGAAGG CCTGGGAGTG GAGGTACCGG 
CTCTACCGGC GCATGAAAAC TGGCTTCGAA CATGCCCGAG CCCCAGAGGT GCCAGCCAAT 
GCCTGTCTCA TGGTAAGCAG CAGCACGTCC CTAACGGTCA GCTTTCAAGA GCCCCTCAGT 
GTCAACGCAG CCGTAGTGAC CAGGTACAAA GTGGAATGGA GCATGTCTAA AGACTTTTCC 
CCGTTGGCTG GGGAGATCAT CATGGATAAC CTGCAGACCC TGAGGTGTAC AATCACAGGA 
CTCACCACGG GCCAACAGTA TTTTGTTCAA GTCTCAGCTT ACAACATGAA AGGATGGGGA 
CCAGCTCAGA CCACAACGCC AGTGTGTGCC TCCCCCTCCA ACTGGAAAGA CTATGACGAC 
AGAGAGCCCA GACACAAGGG ACAGAGTGAG GTTCTGGAGA GCCTACTGCA GCAAGTCCGA 
GCCCTTCATC AGCATTACAG CTGCCGGGAA AGCTCAAAAC TGCAAACCAC AGGCCGCAAG 
CAGTCGGTGT CCAGGAGCCT GAAGCACCTA TTTCATTCCT CAAACAAGTT CGTGAAGACC 
TTGAAACGAG GACTTTACAT AGCCACTATA TTTTATTATA AAGACAATAT GTTAGTCACC 
AATGAAGACC AAATACCAAT TGTTGAAATA GATGACTCTC ACACCAGTTC TATTACACAA 
GACTTTCTGT GGTTCATGAA GCTGTCTTGT ATGTGGGAAG ACATCAGGTG GCTGAGGCAA 
AGCGTGCCCA TATCCATGTC CTCGTCCACT GTGCTGCAAA CTCGGCAAAA GATGCTTGCT 
GCCACAGCTC AGCTACAGAA TTTACTTGGA ACACACAACT TGGGAAGACT TTACTATGAG 
CCCATTAAAG ATCGCCATGG CAACATTCTC ATAGTTACCA TCAGAGAAGT GGAGATGCTT 
TATTCCTTTT TTAATGGCAA ATGGATGCAA ATCTCAAAAC TGCAAAGCCA GAGGAAGTCT 
CTGTCAACAC CCGAAGAGCC AACAGCCTTA GATATTTTAC TAATAACCAT CCAGGATATT 
CTGTCCTACC ACAAGAGGAG CCATCAGCGG CTCTCACCTG GACTGTATCT AGGTTACCTA 
AAGCTGTGTA GCTCTGTGGA TCAGATCAAA GTGCTCGTAA CCCAAAAGCT ACCCAACATT 
CTCTGCCACG TGAAGATCCG TGAAAACCAC AACATTTCCA AAGAGGAGTG GGAGTGGATC 
CAAAAGCTTT CTGGCTCTGA GTCTATGGAA AGTGTGGATC ATAATGCTGA CTGCCCCATG 
CAATTGTTCT TCTACGAGCT CCAGATGGCA GTGGCGGCTC TCCTGAAGCA GATCAATATA 
CCTCGACACC AGGCAAGGAA CTTCCGCCTC TACACACAGG AGGTGTTGGA AATTGGTCAC 
AATGTGTCCT TTCTTCTCTT GCTCCCTGCC TCAGACGACG TCTGTACAGC CCCAGGACAG 
AATAATCCTT ACACCCCGCA CTCAGGGTTT CTTAACCTCC CTCTTCAGAT GTTTGAACTC 
GTTCATTTTT GCAGCTACAG AGAGAAATTT ATTAGTCTGT ATTGCCGCCT TTCTGCTGTT 
GTGGAGCTGG ACTCTCTGAA TACCCAACAG TCCCTGAGGG AGGCAATCTC AGACAGCGAG 
GTCGCAGCTG CCAAACAACG GCACCAGCAA GTGCTGGACT TTATTCAGCA AATTGATGAA 
GTCTGGCGTG AGATGAGATG GATCATGGAT GCTCTACAGT ATGCCCGGTA CAAGCAACCA 
GTCTCTGGCT TGCCCATCAC TAAGCTGATA GACTCCTCCA GTGAGCAGAA TCTAAAGAAG 
ATCAGTTCTA CGTCATCACA CATAGACTGT CTTCCGTCAA CATCCCCATC GCCAGAGATG 
CACAGAAGAA AGGCAGTGAG TGAGTCACAG CCCTGCTCGG ATGAAGAAGG CTGCTCGGAA 
GTCTTCCTCC CCACAGACAG TGACTATGAC TCCAGCGATG CCCTGAGCCC ACGAGACCTG 
GACCTGGTTT ACCTGTCATC ACATGACATA GCCCAGCAGG CCCTCAGCGG CCTCAGTGGC 
AGCGCCCCAG ATGTCCTACA GGTGCATGAC ATGAAGGCCT CCATGGGACC TGGCCAGGAC 
CCTCAGGGCC CAGGGCAGGG TCCTGACACC GACCACTCAT GTGTCGAGTT TCTCCACAGC 
CTGACCCTCA CGGGCTTGGC ACCCAAGAAC CATGCCAAGA TGGTGGCAGG CACACGGCCG 
CCACTGGGCT TCTTGGGAAA GCGGAAGCCA GGCAAGCACC AGCACTATGG TGGCTTCAGC 
CGCCACCACC GCTGGCTGCG CATGCACAGC GAGACGCAGT CCCTGTCACT CTCCGAAGGT 
GTTTACACCC AGCACCTGTC TCAGGCCTGC GGCCTGGCTG AAGATCCTGG GGAGGCAGAG 
GGGCCTGGAC CTGTGGTGGA TGGGCCCCGA GGCCTGCCTC TGGCCCATGC AGCCAGCCTC 
CCAGAGGAGA GGAGGAGCTG CCTCCAAGAC CCAAGGCGGC CCCTGCAGAG AGTCTATGTG 
GAACCCTATT CCGACACACT CGCAGGCCAG GATCCAAAGC TGTGGACCAG TTTGAGCCCT 
ACCCCTGCAG GCCGCAGCAG CCTCCCAAGC TCCACTAGTT CAGAGATGAG TCCAGACCCC 
ACATCTCCAG TTTCAGAAAT ATTAAGTAGT ATGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314739.1
CDS	80..3538
Translation	MCPVMETSPNRRLPFKDRHFTCSKIIGRRFACFAQRLSYRRKQSQCDLLNESTGHLPATCSSAASKSLSWNYCSKMTQQMQNLHLSQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDLDAVQLLLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQDRVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEVPANACLMVSSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSKDFSPLAGEIIMDNLQTLRCTITGLTTGQQYFVQVSAYNMKGWGPAQTTTPVCASPSNWKDYDDREPRHKGQSEVLESLLQQVRALHQHYSCRESSKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIATIFYYKDNMLVTNEDQIPIVEIDDSHTSSITQDFLWFMKLSCMWEDIRWLRQSVPISMSSSTVLQTRQKMLAATAQLQNLLGTHNLGRLYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLSPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENHNISKEEWEWIQKLSGSESMESVDHNADCPMQLFFYELQMAVAALLKQINIPRHQARNFRLYTQEVLEIGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	NM_001327810.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327810.1

ORF Insert Sequence:

ATGTGTCCTG TCATGGAGAC ATCACCAAAC AGGAGGCTGC CCTTCAAAGA CAGACATTTT 
ACTTGCAGCA AAATAATAGG AAGGAGGTTC GCTTGCTTTG CGCAGAGGCT GAGCTACAGG 
AGAAAGCAGA GCCAATGTGA TTTATTGAAT GAAAGCACTG GACACTTACC AGCAACTTGT 
TCCTCTGCTG CCTCGAAGAG CTTAAGCTGG AACTACTGCT CGAAAATGAC CCAGCAAATG 
CAGAACTTAC ATCTTTCCCA GTCAAAAAAA CACAGTGCTC CATCGTCTCC CAATGCTGCC 
AAGCGACTGT ACAGGAACCT CTCGGAGAAG CTGAAGGGCA GTCACTCGTC CTTTGATGAG 
GCCTATTTTA GAACAAGAAC GGATCGGCTG AGTCTCAGAA AGACTTCTGT GAACTTCCAG 
GGCAACGAAG CCATGTTTGA GGCAGTTGAG CAACAGGACC TAGATGCTGT GCAACTCCTC 
CTCTACCAAT ATACACCGGA AGAACTAGAT CTGAACACAC CGAACAGCGA AGGGCTGACA 
CCCCTGGACA TCGCCATCAT GACCAACAAC GTGCCCATTG CGAGGATTCT TCTGCGGACA 
GGAGCCAGAG AAAGTCCGCA CTTTGTCAGC CTGGAGAGCC GAGCAATGCA TCTCAACACT 
CTAGTTCAGG AGGCCCAGGA CAGGGTGAGT GAGCTGTCTG CACAGGTGGA GAATGAAGGC 
TTCACCCTGG ACAACACAGA GAAAGAGAAG CAGCTGAAGG CCTGGGAGTG GAGGTACCGG 
CTCTACCGGC GCATGAAAAC TGGCTTCGAA CATGCCCGAG CCCCAGAGGT GCCAGCCAAT 
GCCTGTCTCA TGGTAAGCAG CAGCACGTCC CTAACGGTCA GCTTTCAAGA GCCCCTCAGT 
GTCAACGCAG CCGTAGTGAC CAGGTACAAA GTGGAATGGA GCATGTCTAA AGACTTTTCC 
CCGTTGGCTG GGGAGATCAT CATGGATAAC CTGCAGACCC TGAGGTGTAC AATCACAGGA 
CTCACCACGG GCCAACAGTA TTTTGTTCAA GTCTCAGCTT ACAACATGAA AGGATGGGGA 
CCAGCTCAGA CCACAACGCC AGTGTGTGCC TCCCCCTCCA ACTGGAAAGA CTATGACGAC 
AGAGAGCCCA GACACAAGGG ACAGAGTGAG GTTCTGGAGA GCCTACTGCA GCAAGTCCGA 
GCCCTTCATC AGCATTACAG CTGCCGGGAA AGCTCAAAAC TGCAAACCAC AGGCCGCAAG 
CAGTCGGTGT CCAGGAGCCT GAAGCACCTA TTTCATTCCT CAAACAAGTT CGTGAAGACC 
TTGAAACGAG GACTTTACAT AGCCACTATA TTTTATTATA AAGACAATAT GTTAGTCACC 
AATGAAGACC AAATACCAAT TGTTGAAATA GATGACTCTC ACACCAGTTC TATTACACAA 
GACTTTCTGT GGTTCATGAA GCTGTCTTGT ATGTGGGAAG ACATCAGGTG GCTGAGGCAA 
AGCGTGCCCA TATCCATGTC CTCGTCCACT GTGCTGCAAA CTCGGCAAAA GATGCTTGCT 
GCCACAGCTC AGCTACAGAA TTTACTTGGA ACACACAACT TGGGAAGACT TTACTATGAG 
CCCATTAAAG ATCGCCATGG CAACATTCTC ATAGTTACCA TCAGAGAAGT GGAGATGCTT 
TATTCCTTTT TTAATGGCAA ATGGATGCAA ATCTCAAAAC TGCAAAGCCA GAGGAAGTCT 
CTGTCAACAC CCGAAGAGCC AACAGCCTTA GATATTTTAC TAATAACCAT CCAGGATATT 
CTGTCCTACC ACAAGAGGAG CCATCAGCGG CTCTCACCTG GACTGTATCT AGGTTACCTA 
AAGCTGTGTA GCTCTGTGGA TCAGATCAAA GTGCTCGTAA CCCAAAAGCT ACCCAACATT 
CTCTGCCACG TGAAGATCCG TGAAAACCAC AACATTTCCA AAGAGGAGTG GGAGTGGATC 
CAAAAGCTTT CTGGCTCTGA GTCTATGGAA AGTGTGGATC ATAATGCTGA CTGCCCCATG 
CAATTGTTCT TCTACGAGCT CCAGATGGCA GTGGCGGCTC TCCTGAAGCA GATCAATATA 
CCTCGACACC AGGCAAGGAA CTTCCGCCTC TACACACAGG AGGTGTTGGA AATTGGTCAC 
AATGTGTCCT TTCTTCTCTT GCTCCCTGCC TCAGACGACG TCTGTACAGC CCCAGGACAG 
AATAATCCTT ACACCCCGCA CTCAGGGTTT CTTAACCTCC CTCTTCAGAT GTTTGAACTC 
GTTCATTTTT GCAGCTACAG AGAGAAATTT ATTAGTCTGT ATTGCCGCCT TTCTGCTGTT 
GTGGAGCTGG ACTCTCTGAA TACCCAACAG TCCCTGAGGG AGGCAATCTC AGACAGCGAG 
GTCGCAGCTG CCAAACAACG GCACCAGCAA GTGCTGGACT TTATTCAGCA AATTGATGAA 
GTCTGGCGTG AGATGAGATG GATCATGGAT GCTCTACAGT ATGCCCGGTA CAAGCAACCA 
GTCTCTGGCT TGCCCATCAC TAAGCTGATA GACTCCTCCA GTGAGCAGAA TCTAAAGAAG 
ATCAGTTCTA CGTCATCACA CATAGACTGT CTTCCGTCAA CATCCCCATC GCCAGAGATG 
CACAGAAGAA AGGCAGTGAG TGAGTCACAG CCCTGCTCGG ATGAAGAAGG CTGCTCGGAA 
GTCTTCCTCC CCACAGACAG TGACTATGAC TCCAGCGATG CCCTGAGCCC ACGAGACCTG 
GACCTGGTTT ACCTGTCATC ACATGACATA GCCCAGCAGG CCCTCAGCGG CCTCAGTGGC 
AGCGCCCCAG ATGTCCTACA GGTGCATGAC ATGAAGGCCT CCATGGGACC TGGCCAGGAC 
CCTCAGGGCC CAGGGCAGGG TCCTGACACC GACCACTCAT GTGTCGAGTT TCTCCACAGC 
CTGACCCTCA CGGGCTTGGC ACCCAAGAAC CATGCCAAGA TGGTGGCAGG CACACGGCCG 
CCACTGGGCT TCTTGGGAAA GCGGAAGCCA GGCAAGCACC AGCACTATGG TGGCTTCAGC 
CGCCACCACC GCTGGCTGCG CATGCACAGC GAGACGCAGT CCCTGTCACT CTCCGAAGGT 
GTTTACACCC AGCACCTGTC TCAGGCCTGC GGCCTGGCTG AAGATCCTGG GGAGGCAGAG 
GGGCCTGGAC CTGTGGTGGA TGGGCCCCGA GGCCTGCCTC TGGCCCATGC AGCCAGCCTC 
CCAGAGGAGA GGAGGAGCTG CCTCCAAGAC CCAAGGCGGC CCCTGCAGAG AGTCTATGTG 
GAACCCTATT CCGACACACT CGCAGGCCAG GATCCAAAGC TGTGGACCAG TTTGAGCCCT 
ACCCCTGCAG GCCGCAGCAG CCTCCCAAGC TCCACTAGTT CAGAGATGAG TCCAGACCCC 
ACATCTCCAG TTTCAGAAAT ATTAAGTAGT ATGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfn1 ( XM_017314636.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170125.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77962
Clone ID Related Accession (Same CDS sequence)	XM_017314636.1 , XM_030246105.1 , XM_017314636.2 , XM_030246107.1 , XM_030246106.1
Accession Version	XM_017314636.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170125.1
CDS	310..3543
Translation	MTQQMQNLHLSQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDLDAVQLLLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQDRVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEVPANACLMVSSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSKDFSPLAGEIIMDNLQTLRCTITGLTTGQQYFVQVSAYNMKGWGPAQTTTPVCASPSNWKDYDDREPRHKGQSEVLESLLQQVRALHQHYSCRESSKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIATIFYYKDNMLVTNEDQIPIVEIDDSHTSSITQDFLWFMKLSCMWEDIRWLRQSVPISMSSSTVLQTRQKMLAATAQLQNLLGTHNLGRLYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLSPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENHNISKEEWEWIQKLSGSESMESVDHNADCPMQLFFYELQMAVAALLKQINIPRHQARNFRLYTQEVLEIGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	XM_017314636.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314636.1

ORF Insert Sequence:

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfn1 ( XM_030246105.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101965.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77962
Clone ID Related Accession (Same CDS sequence)	XM_017314636.1 , XM_030246105.1 , XM_017314636.2 , XM_030246107.1 , XM_030246106.1
Accession Version	XM_030246105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101965.1
CDS	413..3646
Translation	MTQQMQNLHLSQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDLDAVQLLLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQDRVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEVPANACLMVSSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSKDFSPLAGEIIMDNLQTLRCTITGLTTGQQYFVQVSAYNMKGWGPAQTTTPVCASPSNWKDYDDREPRHKGQSEVLESLLQQVRALHQHYSCRESSKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIATIFYYKDNMLVTNEDQIPIVEIDDSHTSSITQDFLWFMKLSCMWEDIRWLRQSVPISMSSSTVLQTRQKMLAATAQLQNLLGTHNLGRLYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLSPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENHNISKEEWEWIQKLSGSESMESVDHNADCPMQLFFYELQMAVAALLKQINIPRHQARNFRLYTQEVLEIGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	XM_030246105.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246105.1

ORF Insert Sequence:

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfn1 ( XM_017314636.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170125.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77962
Clone ID Related Accession (Same CDS sequence)	XM_017314636.1 , XM_030246105.1 , XM_017314636.2 , XM_030246107.1 , XM_030246106.1
Accession Version	XM_017314636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170125.1
CDS	310..3543
Translation	MTQQMQNLHLSQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDLDAVQLLLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQDRVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEVPANACLMVSSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSKDFSPLAGEIIMDNLQTLRCTITGLTTGQQYFVQVSAYNMKGWGPAQTTTPVCASPSNWKDYDDREPRHKGQSEVLESLLQQVRALHQHYSCRESSKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIATIFYYKDNMLVTNEDQIPIVEIDDSHTSSITQDFLWFMKLSCMWEDIRWLRQSVPISMSSSTVLQTRQKMLAATAQLQNLLGTHNLGRLYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLSPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENHNISKEEWEWIQKLSGSESMESVDHNADCPMQLFFYELQMAVAALLKQINIPRHQARNFRLYTQEVLEIGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	XM_017314636.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314636.2

ORF Insert Sequence:

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfn1 ( XM_030246107.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101967.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu77962
Clone ID Related Accession (Same CDS sequence)	XM_017314636.1 , XM_030246105.1 , XM_017314636.2 , XM_030246107.1 , XM_030246106.1
Accession Version	XM_030246107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101967.1
CDS	480..3713
Translation	MTQQMQNLHLSQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDLDAVQLLLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQDRVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEVPANACLMVSSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSKDFSPLAGEIIMDNLQTLRCTITGLTTGQQYFVQVSAYNMKGWGPAQTTTPVCASPSNWKDYDDREPRHKGQSEVLESLLQQVRALHQHYSCRESSKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIATIFYYKDNMLVTNEDQIPIVEIDDSHTSSITQDFLWFMKLSCMWEDIRWLRQSVPISMSSSTVLQTRQKMLAATAQLQNLLGTHNLGRLYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLSPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENHNISKEEWEWIQKLSGSESMESVDHNADCPMQLFFYELQMAVAALLKQINIPRHQARNFRLYTQEVLEIGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	XM_030246107.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246107.1

ORF Insert Sequence:

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfn1 ( XM_030246106.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101966.1 Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu77962
Clone ID Related Accession (Same CDS sequence)	XM_017314636.1 , XM_030246105.1 , XM_017314636.2 , XM_030246107.1 , XM_030246106.1
Accession Version	XM_030246106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101966.1
CDS	326..3559
Translation	MTQQMQNLHLSQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDLDAVQLLLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQDRVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEVPANACLMVSSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSKDFSPLAGEIIMDNLQTLRCTITGLTTGQQYFVQVSAYNMKGWGPAQTTTPVCASPSNWKDYDDREPRHKGQSEVLESLLQQVRALHQHYSCRESSKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIATIFYYKDNMLVTNEDQIPIVEIDDSHTSSITQDFLWFMKLSCMWEDIRWLRQSVPISMSSSTVLQTRQKMLAATAQLQNLLGTHNLGRLYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLSPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENHNISKEEWEWIQKLSGSESMESVDHNADCPMQLFFYELQMAVAALLKQINIPRHQARNFRLYTQEVLEIGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDSSSEQNLKKISSTSSHIDCLPSTSPSPEMHRRKAVSESQPCSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYLSSHDIAQQALSGLSGSAPDVLQVHDMKASMGPGQDPQGPGQGPDTDHSCVEFLHSLTLTGLAPKNHAKMVAGTRPPLGFLGKRKPGKHQHYGGFSRHHRWLRMHSETQSLSLSEGVYTQHLSQACGLAEDPGEAEGPGPVVDGPRGLPLAHAASLPEERRSCLQDPRRPLQRVYVEPYSDTLAGQDPKLWTSLSPTPAGRSSLPSSTSSEMSPDPTSPVSEILSSML

Target ORF information:

RefSeq Version	XM_030246106.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin-repeat and fibronectin type III domain containing 1 (Ankfn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246106.1

ORF Insert Sequence:

ATGACCCAGC AAATGCAGAA CTTACATCTT TCCCAGTCAA AAAAACACAG TGCTCCATCG 
TCTCCCAATG CTGCCAAGCG ACTGTACAGG AACCTCTCGG AGAAGCTGAA GGGCAGTCAC 
TCGTCCTTTG ATGAGGCCTA TTTTAGAACA AGAACGGATC GGCTGAGTCT CAGAAAGACT 
TCTGTGAACT TCCAGGGCAA CGAAGCCATG TTTGAGGCAG TTGAGCAACA GGACCTAGAT 
GCTGTGCAAC TCCTCCTCTA CCAATATACA CCGGAAGAAC TAGATCTGAA CACACCGAAC 
AGCGAAGGGC TGACACCCCT GGACATCGCC ATCATGACCA ACAACGTGCC CATTGCGAGG 
ATTCTTCTGC GGACAGGAGC CAGAGAAAGT CCGCACTTTG TCAGCCTGGA GAGCCGAGCA 
ATGCATCTCA ACACTCTAGT TCAGGAGGCC CAGGACAGGG TGAGTGAGCT GTCTGCACAG 
GTGGAGAATG AAGGCTTCAC CCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAGGCCTGG 
GAGTGGAGGT ACCGGCTCTA CCGGCGCATG AAAACTGGCT TCGAACATGC CCGAGCCCCA 
GAGGTGCCAG CCAATGCCTG TCTCATGGTA AGCAGCAGCA CGTCCCTAAC GGTCAGCTTT 
CAAGAGCCCC TCAGTGTCAA CGCAGCCGTA GTGACCAGGT ACAAAGTGGA ATGGAGCATG 
TCTAAAGACT TTTCCCCGTT GGCTGGGGAG ATCATCATGG ATAACCTGCA GACCCTGAGG 
TGTACAATCA CAGGACTCAC CACGGGCCAA CAGTATTTTG TTCAAGTCTC AGCTTACAAC 
ATGAAAGGAT GGGGACCAGC TCAGACCACA ACGCCAGTGT GTGCCTCCCC CTCCAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAGGTTCT GGAGAGCCTA 
CTGCAGCAAG TCCGAGCCCT TCATCAGCAT TACAGCTGCC GGGAAAGCTC AAAACTGCAA 
ACCACAGGCC GCAAGCAGTC GGTGTCCAGG AGCCTGAAGC ACCTATTTCA TTCCTCAAAC 
AAGTTCGTGA AGACCTTGAA ACGAGGACTT TACATAGCCA CTATATTTTA TTATAAAGAC 
AATATGTTAG TCACCAATGA AGACCAAATA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGACTT TCTGTGGTTC ATGAAGCTGT CTTGTATGTG GGAAGACATC 
AGGTGGCTGA GGCAAAGCGT GCCCATATCC ATGTCCTCGT CCACTGTGCT GCAAACTCGG 
CAAAAGATGC TTGCTGCCAC AGCTCAGCTA CAGAATTTAC TTGGAACACA CAACTTGGGA 
AGACTTTACT ATGAGCCCAT TAAAGATCGC CATGGCAACA TTCTCATAGT TACCATCAGA 
GAAGTGGAGA TGCTTTATTC CTTTTTTAAT GGCAAATGGA TGCAAATCTC AAAACTGCAA 
AGCCAGAGGA AGTCTCTGTC AACACCCGAA GAGCCAACAG CCTTAGATAT TTTACTAATA 
ACCATCCAGG ATATTCTGTC CTACCACAAG AGGAGCCATC AGCGGCTCTC ACCTGGACTG 
TATCTAGGTT ACCTAAAGCT GTGTAGCTCT GTGGATCAGA TCAAAGTGCT CGTAACCCAA 
AAGCTACCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACCACAACAT TTCCAAAGAG 
GAGTGGGAGT GGATCCAAAA GCTTTCTGGC TCTGAGTCTA TGGAAAGTGT GGATCATAAT 
GCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGGC GGCTCTCCTG 
AAGCAGATCA ATATACCTCG ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATTG GTCACAATGT GTCCTTTCTT CTCTTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCGCACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTCGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGACTCT CTGAATACCC AACAGTCCCT GAGGGAGGCA 
ATCTCAGACA GCGAGGTCGC AGCTGCCAAA CAACGGCACC AGCAAGTGCT GGACTTTATT 
CAGCAAATTG ATGAAGTCTG GCGTGAGATG AGATGGATCA TGGATGCTCT ACAGTATGCC 
CGGTACAAGC AACCAGTCTC TGGCTTGCCC ATCACTAAGC TGATAGACTC CTCCAGTGAG 
CAGAATCTAA AGAAGATCAG TTCTACGTCA TCACACATAG ACTGTCTTCC GTCAACATCC 
CCATCGCCAG AGATGCACAG AAGAAAGGCA GTGAGTGAGT CACAGCCCTG CTCGGATGAA 
GAAGGCTGCT CGGAAGTCTT CCTCCCCACA GACAGTGACT ATGACTCCAG CGATGCCCTG 
AGCCCACGAG ACCTGGACCT GGTTTACCTG TCATCACATG ACATAGCCCA GCAGGCCCTC 
AGCGGCCTCA GTGGCAGCGC CCCAGATGTC CTACAGGTGC ATGACATGAA GGCCTCCATG 
GGACCTGGCC AGGACCCTCA GGGCCCAGGG CAGGGTCCTG ACACCGACCA CTCATGTGTC 
GAGTTTCTCC ACAGCCTGAC CCTCACGGGC TTGGCACCCA AGAACCATGC CAAGATGGTG 
GCAGGCACAC GGCCGCCACT GGGCTTCTTG GGAAAGCGGA AGCCAGGCAA GCACCAGCAC 
TATGGTGGCT TCAGCCGCCA CCACCGCTGG CTGCGCATGC ACAGCGAGAC GCAGTCCCTG 
TCACTCTCCG AAGGTGTTTA CACCCAGCAC CTGTCTCAGG CCTGCGGCCT GGCTGAAGAT 
CCTGGGGAGG CAGAGGGGCC TGGACCTGTG GTGGATGGGC CCCGAGGCCT GCCTCTGGCC 
CATGCAGCCA GCCTCCCAGA GGAGAGGAGG AGCTGCCTCC AAGACCCAAG GCGGCCCCTG 
CAGAGAGTCT ATGTGGAACC CTATTCCGAC ACACTCGCAG GCCAGGATCC AAAGCTGTGG 
ACCAGTTTGA GCCCTACCCC TGCAGGCCGC AGCAGCCTCC CAAGCTCCAC TAGTTCAGAG 
ATGAGTCCAG ACCCCACATC TCCAGTTTCA GAAATATTAA GTAGTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies.
PLoS genetics11(7)e1005344(2015 Jul)
Zhang S,Ross KD,Seidner GA,Gorman MR,Poon TH,Wang X,Keithley EM,Lee PN,Martindale MQ,Joiner WJ,Hamilton BA

Mutation rate and predicted phenotypic target sizes in ethylnitrosourea-treated mice.
Genetics168(2)953-9(2004 Oct)
Concepcion D,Seburn KL,Wen G,Frankel WN,Hamilton BA

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Ankfn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報