ANKFN1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017024263.1	XP_016879752.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_017024264.1	XP_016879753.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_017024265.2	XP_016879754.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_011524430.2	XP_011522732.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
XM_011524431.2	XP_011522733.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
XM_017024270.1	XP_016879759.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X8
NM_001370326.1	NP_001357255.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 3
XM_011524433.2	XP_011522735.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X7
XM_017024265.1	XP_016879754.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_017024266.1	XP_016879755.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X2
XM_017024269.1	XP_016879758.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
XM_011524434.2	XP_011522736.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X9
XM_017024268.1	XP_016879757.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
NM_153228.2	NP_694960.2	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 2
XM_017024267.1	XP_016879756.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X5
XM_017024270.2	XP_016879759.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X8
NM_001365758.1	NP_001352687.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 1
XM_011524428.2	XP_011522730.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X3
XM_011524429.2	XP_011522731.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X4
XM_006721728.3	XP_006721791.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13303	NM_153228.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$384.50-$538.30 $769.00
OHu77169	XM_011524433.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu77170	XM_011524434.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu86639	XM_017024270.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu108547	NM_001370326.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu86636	XM_017024263.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu86636	XM_017024264.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu86636	XM_017024265.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu86637	XM_017024266.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu86638	XM_017024267.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu50152	NM_001365758.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu50152	XM_011524430.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu50152	XM_011524431.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu50152	XM_017024268.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu50152	XM_006721728.3 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu50152	XM_017024269.1 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu77166	XM_011524428.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu77167	XM_011524429.2 Latest version!	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu86636	XM_017024265.1	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu86639	XM_017024270.1	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ANKFN1 ( NM_153228.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_694960.2 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13303
Clone ID Related Accession (Same CDS sequence)	NM_153228.2
Accession Version	NM_153228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-11
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC006925.6, AC006600.4 and AC015724.9. On Sep 20, 2006 this sequence version replaced NM_153228.1. ##Evidence-Data-START## Transcript exon combination :: AK095654.1, SRR1803612.140164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCGT CTCTAACCAG GAGGCTACTC TTTAAAGACA GGCATTTTAC TTGCAGCAAA 
ATAATAGGAA GGAGATTCGC TTGCTTTGCA CAGAGGCTGA GCCACAGGAG AAAGCAAAGC 
CAATGTGATT TATTGAATGA AAGCACTGGA CAATTACCAA CAACTTGTTC CTCTGCTGCC 
TCGAACAGCA TAAACTGGAA TTGTCGTGTG AAAATGACGC AACAAATGCA AAATTTACAT 
CTCTGTCAGT CAAAAAAACA TAGTGCTCCC TCATCTCCCA ACGCAGCCAA ACGCCTGTAC 
AGGAACCTCT CTGAGAAACT GAAAGGGAGC CACTCTTCCT TCGATGAGGC CTATTTTAGG 
ACAAGAACTG ATCGGCTGAG TCTCAGGAAG ACCTCGGTGA ATTTCCAGGG CAATGAAGCC 
ATGTTTGAGG CAGTCGAACA GCAGGACATG GATGCTGTGC AGATCCTCCT GTATCAGTAC 
ACACCAGAAG AACTTGACCT CAACACACCT AACAGCGAGG GCTTGACACC CCTGGATATT 
GCCATCATGA CCAACAATGT GCCCATTGCA AGGATTCTTC TGAGGACAGG GGCCCGAGAA 
AGTCCACACT TTGTCAGCCT GGAAAGCCGA GCAATGCACC TCAACACACT GGTCCAGGAA 
GCCCAGGAGA GGGTGAGTGA ACTGTCTGCC CAGGTGGAGA ATGAAGGATT CACTCTGGAC 
AACACAGAGA AAGAGAAGCA GCTGAAAGCT TGGGAGTGGA GGTATCGGCT CTACAGACGC 
ATGAAAACAG GCTTTGAGCA TGCCAGAGCC CCTGAGATGC CAACCAATGT CTGTCTCATG 
GTAACCAGCA GCACATCACT CACTGTCAGC TTCCAAGAGC CTCTTAGCGT CAATGCAGCT 
GTAGTAACCA GGTATAAAGT GGAATGGAGT ATGTCCGAAG ACTTTTCTCC TTTGGCTGGA 
GAAATCATCA TGGATAATCT GCAGACTCTG AGATGCACAA TCACAGGACT TACAATGGGC 
CAACAGTATT TTGTTCAAGT CTCGGCTTAC AATATGAAAG GATGGGGACC TGCTCAGACC 
ACGACACCGG CATGTGCCTC TCCTTCTAAC TGGAAAGACT ATGACGACAG AGAGCCCAGA 
CACAAGGGAC AGAGTGAAGT TTTGGAAGGT CTGCTGCAGC AGGTCCGAGC CCTTCATCAG 
CATTACAGTT GCCGGGAAAG CACAAAATTA CAAACCACAG GCCGCAAGCA GTCAGTCTCA 
AGAAGCCTGA AACACCTGTT CCATTCCTCG AACAAGTTTG TGAAGACCTT AAAACGGGGA 
CTCTACATAG CCGTTATATT TTATTACAAA GACAATATCT TAGTCACCAA TGAAGATCAA 
GTACCAATTG TTGAAATAGA TGACTCTCAC ACCAGTTCTA TTACACAAGA TTTTCTGTGG 
TTCACGAAGC TGTCTTGTAT GTGGGAAGAT ATAAGGTGGC TGAGGCAAAG CATACCAATA 
TCCTCATCCT CATCCACAGT GCTGCAAACT CGGCAGAAGA TGCTCGCAGC AACAGCACAG 
CTACAGAATT TACTTGGGAC ACACAACTTG GGAAGAGTTT ACTATGAGCC CATTAAAGAT 
CGACATGGAA ACATACTCAT AGTCACCATC AGGGAGGTGG AGATGCTTTA TTCATTTTTT 
AATGGCAAAT GGATGCAGAT CTCAAAGCTG CAAAGCCAGA GAAAGTCTCT ATCAACACCT 
GAGGAGCCAA CAGCTTTAGA CATTCTACTG ATAACCATCC AGGATATTCT ATCCTATCAC 
AAAAGGAGTC ATCAGCGTCT CTTTCCTGGA TTATATCTGG GTTACCTAAA GCTCTGTAGC 
TCTGTGGATC AAATCAAAGT TCTTGTTACC CAAAAGTTGC CCAACATTCT CTGCCACGTG 
AAGATCCGTG AAAACAATAA TATTTCTAGA GAGGAATGGG AATGGATCCA AAAGCTTTCT 
GGCTCTGAAT CTATGGAAAG TGTGGATCAT ACTTCTGACT GCCCCATGCA ATTGTTCTTC 
TACGAGCTCC AGATGGCAGT GAAAGCTCTC CTTCAGCAGA TCAATATACC TCTACACCAG 
GCAAGGAACT TCCGCCTCTA CACACAGGAG GTGTTGGAAA TGGGTCACAA TGTGTCCTTT 
CTTCTCCTGC TCCCTGCCTC AGACGACGTC TGTACAGCCC CAGGACAGAA TAATCCTTAC 
ACCCCACACT CAGGGTTTCT TAACCTCCCT CTTCAGATGT TTGAACTTGG TATAGTAGCT 
TGTTTCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694960.2
CDS	36..2327
Translation	MEASLTRRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELGIVACFT

Target ORF information:

RefSeq Version	NM_153228.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153228.2

ORF Insert Sequence:

ATGGAGGCGT CTCTAACCAG GAGGCTACTC TTTAAAGACA GGCATTTTAC TTGCAGCAAA 
ATAATAGGAA GGAGATTCGC TTGCTTTGCA CAGAGGCTGA GCCACAGGAG AAAGCAAAGC 
CAATGTGATT TATTGAATGA AAGCACTGGA CAATTACCAA CAACTTGTTC CTCTGCTGCC 
TCGAACAGCA TAAACTGGAA TTGTCGTGTG AAAATGACGC AACAAATGCA AAATTTACAT 
CTCTGTCAGT CAAAAAAACA TAGTGCTCCC TCATCTCCCA ACGCAGCCAA ACGCCTGTAC 
AGGAACCTCT CTGAGAAACT GAAAGGGAGC CACTCTTCCT TCGATGAGGC CTATTTTAGG 
ACAAGAACTG ATCGGCTGAG TCTCAGGAAG ACCTCGGTGA ATTTCCAGGG CAATGAAGCC 
ATGTTTGAGG CAGTCGAACA GCAGGACATG GATGCTGTGC AGATCCTCCT GTATCAGTAC 
ACACCAGAAG AACTTGACCT CAACACACCT AACAGCGAGG GCTTGACACC CCTGGATATT 
GCCATCATGA CCAACAATGT GCCCATTGCA AGGATTCTTC TGAGGACAGG GGCCCGAGAA 
AGTCCACACT TTGTCAGCCT GGAAAGCCGA GCAATGCACC TCAACACACT GGTCCAGGAA 
GCCCAGGAGA GGGTGAGTGA ACTGTCTGCC CAGGTGGAGA ATGAAGGATT CACTCTGGAC 
AACACAGAGA AAGAGAAGCA GCTGAAAGCT TGGGAGTGGA GGTATCGGCT CTACAGACGC 
ATGAAAACAG GCTTTGAGCA TGCCAGAGCC CCTGAGATGC CAACCAATGT CTGTCTCATG 
GTAACCAGCA GCACATCACT CACTGTCAGC TTCCAAGAGC CTCTTAGCGT CAATGCAGCT 
GTAGTAACCA GGTATAAAGT GGAATGGAGT ATGTCCGAAG ACTTTTCTCC TTTGGCTGGA 
GAAATCATCA TGGATAATCT GCAGACTCTG AGATGCACAA TCACAGGACT TACAATGGGC 
CAACAGTATT TTGTTCAAGT CTCGGCTTAC AATATGAAAG GATGGGGACC TGCTCAGACC 
ACGACACCGG CATGTGCCTC TCCTTCTAAC TGGAAAGACT ATGACGACAG AGAGCCCAGA 
CACAAGGGAC AGAGTGAAGT TTTGGAAGGT CTGCTGCAGC AGGTCCGAGC CCTTCATCAG 
CATTACAGTT GCCGGGAAAG CACAAAATTA CAAACCACAG GCCGCAAGCA GTCAGTCTCA 
AGAAGCCTGA AACACCTGTT CCATTCCTCG AACAAGTTTG TGAAGACCTT AAAACGGGGA 
CTCTACATAG CCGTTATATT TTATTACAAA GACAATATCT TAGTCACCAA TGAAGATCAA 
GTACCAATTG TTGAAATAGA TGACTCTCAC ACCAGTTCTA TTACACAAGA TTTTCTGTGG 
TTCACGAAGC TGTCTTGTAT GTGGGAAGAT ATAAGGTGGC TGAGGCAAAG CATACCAATA 
TCCTCATCCT CATCCACAGT GCTGCAAACT CGGCAGAAGA TGCTCGCAGC AACAGCACAG 
CTACAGAATT TACTTGGGAC ACACAACTTG GGAAGAGTTT ACTATGAGCC CATTAAAGAT 
CGACATGGAA ACATACTCAT AGTCACCATC AGGGAGGTGG AGATGCTTTA TTCATTTTTT 
AATGGCAAAT GGATGCAGAT CTCAAAGCTG CAAAGCCAGA GAAAGTCTCT ATCAACACCT 
GAGGAGCCAA CAGCTTTAGA CATTCTACTG ATAACCATCC AGGATATTCT ATCCTATCAC 
AAAAGGAGTC ATCAGCGTCT CTTTCCTGGA TTATATCTGG GTTACCTAAA GCTCTGTAGC 
TCTGTGGATC AAATCAAAGT TCTTGTTACC CAAAAGTTGC CCAACATTCT CTGCCACGTG 
AAGATCCGTG AAAACAATAA TATTTCTAGA GAGGAATGGG AATGGATCCA AAAGCTTTCT 
GGCTCTGAAT CTATGGAAAG TGTGGATCAT ACTTCTGACT GCCCCATGCA ATTGTTCTTC 
TACGAGCTCC AGATGGCAGT GAAAGCTCTC CTTCAGCAGA TCAATATACC TCTACACCAG 
GCAAGGAACT TCCGCCTCTA CACACAGGAG GTGTTGGAAA TGGGTCACAA TGTGTCCTTT 
CTTCTCCTGC TCCCTGCCTC AGACGACGTC TGTACAGCCC CAGGACAGAA TAATCCTTAC 
ACCCCACACT CAGGGTTTCT TAACCTCCCT CTTCAGATGT TTGAACTTGG TATAGTAGCT 
TGTTTCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_011524433.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522735.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu77169
Clone ID Related Accession (Same CDS sequence)	XM_011524433.2
Accession Version	XM_011524433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG ACTTTTCTCC TTTGGCTGGA GAAATCATCA TGGATAATCT GCAGACTCTG 
AGATGCACAA TCACAGGACT TACAATGGGC CAACAGTATT TTGTTCAAGT CTCGGCTTAC 
AATATGAAAG GATGGGGACC TGCTCAGACC ACGACACCGG CATGTGCCTC TCCTTCTAAC 
TGGAAAGACT ATGACGACAG AGAGCCCAGA CACAAGGGAC AGAGTGAAGT TTTGGAAGGT 
CTGCTGCAGC AGGTCCGAGC CCTTCATCAG CATTACAGTT GCCGGGAAAG CACAAAATTA 
CAAACCACAG GCCGCAAGCA GTCAGTCTCA AGAAGCCTGA AACACCTGTT CCATTCCTCG 
AACAAGTTTG TGAAGACCTT AAAACGGGGA CTCTACATAG CCGTTATATT TTATTACAAA 
GACAATATCT TAGTCACCAA TGAAGATCAA GTACCAATTG TTGAAATAGA TGACTCTCAC 
ACCAGTTCTA TTACACAAGA TTTTCTGTGG TTCACGAAGC TGTCTTGTAT GTGGGAAGAT 
ATAAGGTGGC TGAGGCAAAG CATACCAATA TCCTCATCCT CATCCACAGT GCTGCAAACT 
CGGCAGAAGA TGCTCGCAGC AACAGCACAG CTACAGAATT TACTTGGGAC ACACAACTTG 
GGAAGAGTTT ACTATGAGCC CATTAAAGAT CGACATGGAA ACATACTCAT AGTCACCATC 
AGGGAGGTGG AGATGCTTTA TTCATTTTTT AATGGCAAAT GGATGCAGAT CTCAAAGCTG 
CAAAGCCAGA GAAAGTCTCT ATCAACACCT GAGGAGCCAA CAGCTTTAGA CATTCTACTG 
ATAACCATCC AGGATATTCT ATCCTATCAC AAAAGGAGTC ATCAGCGTCT CTTTCCTGGA 
TTATATCTGG GTTACCTAAA GCTCTGTAGC TCTGTGGATC AAATCAAAGT TCTTGTTACC 
CAAAAGTTGC CCAACATTCT CTGCCACGTG AAGATCCGTG AAAACAATAA TATTTCTAGA 
GAGGAATGGG AATGGATCCA AAAGCTTTCT GGCTCTGAAT CTATGGAAAG TGTGGATCAT 
ACTTCTGACT GCCCCATGCA ATTGTTCTTC TACGAGCTCC AGATGGCAGT GAAAGCTCTC 
CTTCAGCAGA TCAATATACC TCTACACCAG GCAAGGAACT TCCGCCTCTA CACACAGGAG 
GTGTTGGAAA TGGGTCACAA TGTGTCCTTT CTTCTCCTGC TCCCTGCCTC AGACGACGTC 
TGTACAGCCC CAGGACAGAA TAATCCTTAC ACCCCACACT CAGGGTTTCT TAACCTCCCT 
CTTCAGATGT TTGAACTTGT TCATTTTTGC AGCTACAGAG AGAAATTTAT TAGTCTGTAT 
TGCCGCCTTT CTGCTGTTGT GGAGCTGGAT TCTCTGAACA CCCAACAGTC CCTCAGGGAA 
GCAATCTCAG ACAGCGAGGT TGCAGCTGCC AAACAAAGAC ACCAGCAAGT TTTAGATTTC 
ATTCAGCAAA TAGATGAAGT CTGGCGTGAA ATGAGATGGA TCATGGATGC TCTACAGTAT 
GCAAGATACA AACAACCAGT TTCTGGCTTG CCCATCACTA AGCTGATAGA CCCCTCAGAT 
GAGCAGAGCC TAAAGAAGAT CAATTCTACA TCATCATCAC ATATAGACTG TCTTCCATCC 
CCACCCCCAT CCCCAGAGAT GCACAGAAGA AAGACAGTGA GTGATTCACA GCCCTGCTCT 
GATGAAGAAG CCTGCTCAGA AGTCTTCCTC CCCACCAACA GTGACTACGA CTCCAGCGAT 
GCCCTGAGCC CCAGAGACCT GGACCTGGTC TACCTATCAT CTCACGACAT TGCGCAGCAG 
ACCCTTAGCG GCCTAAGCGG CAGCGCCCCC GACGTCCTGC AAGTGCACGA CGTGAAAACC 
CCTCTGGGGC CGGGCCAGGA TCCCCAGGGC GAGGGCCCAA ATCCCGATCA CTCATGTGCC 
GAGTTTCTCC ATAGCCTGAC CCTCACGGGG TTCACACCCA AGAACCACGC CAAGACTGTG 
TCCGGTGGGC GGCCCCCGCT AGGCTTCCTG GGAAAGCGGA AGCCAGGCAA GCACCCCCAC 
TATGGCGGCT TCAGCCGCCA TCATCGCTGG TTGCGCATCC ACAGCGAGAC CCAGTCGCTA 
TCGCTCTCTG AGGGCATTTA TACACAGCAC CTGTCCCAGG CCTGTGGTCT GGCCCAGGAG 
CCCAAGGAGG CCAAGCGGGC CGGCCCTGCC CTTGATGATC CCAGGGGCCT AACTCTGGCC 
CACGCTGCCA GCCTTCCTGA GGAGCGGAAC AGCAGTCTCC AGGACGCGAG GCCTTCCGTC 
CGCCGCCTCT ACGTGGAGCC CTACGCAGCG GCCGTGGTGG CCCAGGACGA AAAACCATGG 
GCAAGCTTGA GCCCGCCCTC TGGAGGCCGC ATCACCCTGC CCAGCCCCAC TGGCCCCGAT 
GTGAGTCAGG AGGGCCCCAC CGCCTCTCCC ATGTCAGAAA TACTCAGCAG CATGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522735.1
CDS	379..2898
Translation	MSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_011524433.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524433.2

ORF Insert Sequence:

ATGTCCGAAG ACTTTTCTCC TTTGGCTGGA GAAATCATCA TGGATAATCT GCAGACTCTG 
AGATGCACAA TCACAGGACT TACAATGGGC CAACAGTATT TTGTTCAAGT CTCGGCTTAC 
AATATGAAAG GATGGGGACC TGCTCAGACC ACGACACCGG CATGTGCCTC TCCTTCTAAC 
TGGAAAGACT ATGACGACAG AGAGCCCAGA CACAAGGGAC AGAGTGAAGT TTTGGAAGGT 
CTGCTGCAGC AGGTCCGAGC CCTTCATCAG CATTACAGTT GCCGGGAAAG CACAAAATTA 
CAAACCACAG GCCGCAAGCA GTCAGTCTCA AGAAGCCTGA AACACCTGTT CCATTCCTCG 
AACAAGTTTG TGAAGACCTT AAAACGGGGA CTCTACATAG CCGTTATATT TTATTACAAA 
GACAATATCT TAGTCACCAA TGAAGATCAA GTACCAATTG TTGAAATAGA TGACTCTCAC 
ACCAGTTCTA TTACACAAGA TTTTCTGTGG TTCACGAAGC TGTCTTGTAT GTGGGAAGAT 
ATAAGGTGGC TGAGGCAAAG CATACCAATA TCCTCATCCT CATCCACAGT GCTGCAAACT 
CGGCAGAAGA TGCTCGCAGC AACAGCACAG CTACAGAATT TACTTGGGAC ACACAACTTG 
GGAAGAGTTT ACTATGAGCC CATTAAAGAT CGACATGGAA ACATACTCAT AGTCACCATC 
AGGGAGGTGG AGATGCTTTA TTCATTTTTT AATGGCAAAT GGATGCAGAT CTCAAAGCTG 
CAAAGCCAGA GAAAGTCTCT ATCAACACCT GAGGAGCCAA CAGCTTTAGA CATTCTACTG 
ATAACCATCC AGGATATTCT ATCCTATCAC AAAAGGAGTC ATCAGCGTCT CTTTCCTGGA 
TTATATCTGG GTTACCTAAA GCTCTGTAGC TCTGTGGATC AAATCAAAGT TCTTGTTACC 
CAAAAGTTGC CCAACATTCT CTGCCACGTG AAGATCCGTG AAAACAATAA TATTTCTAGA 
GAGGAATGGG AATGGATCCA AAAGCTTTCT GGCTCTGAAT CTATGGAAAG TGTGGATCAT 
ACTTCTGACT GCCCCATGCA ATTGTTCTTC TACGAGCTCC AGATGGCAGT GAAAGCTCTC 
CTTCAGCAGA TCAATATACC TCTACACCAG GCAAGGAACT TCCGCCTCTA CACACAGGAG 
GTGTTGGAAA TGGGTCACAA TGTGTCCTTT CTTCTCCTGC TCCCTGCCTC AGACGACGTC 
TGTACAGCCC CAGGACAGAA TAATCCTTAC ACCCCACACT CAGGGTTTCT TAACCTCCCT 
CTTCAGATGT TTGAACTTGT TCATTTTTGC AGCTACAGAG AGAAATTTAT TAGTCTGTAT 
TGCCGCCTTT CTGCTGTTGT GGAGCTGGAT TCTCTGAACA CCCAACAGTC CCTCAGGGAA 
GCAATCTCAG ACAGCGAGGT TGCAGCTGCC AAACAAAGAC ACCAGCAAGT TTTAGATTTC 
ATTCAGCAAA TAGATGAAGT CTGGCGTGAA ATGAGATGGA TCATGGATGC TCTACAGTAT 
GCAAGATACA AACAACCAGT TTCTGGCTTG CCCATCACTA AGCTGATAGA CCCCTCAGAT 
GAGCAGAGCC TAAAGAAGAT CAATTCTACA TCATCATCAC ATATAGACTG TCTTCCATCC 
CCACCCCCAT CCCCAGAGAT GCACAGAAGA AAGACAGTGA GTGATTCACA GCCCTGCTCT 
GATGAAGAAG CCTGCTCAGA AGTCTTCCTC CCCACCAACA GTGACTACGA CTCCAGCGAT 
GCCCTGAGCC CCAGAGACCT GGACCTGGTC TACCTATCAT CTCACGACAT TGCGCAGCAG 
ACCCTTAGCG GCCTAAGCGG CAGCGCCCCC GACGTCCTGC AAGTGCACGA CGTGAAAACC 
CCTCTGGGGC CGGGCCAGGA TCCCCAGGGC GAGGGCCCAA ATCCCGATCA CTCATGTGCC 
GAGTTTCTCC ATAGCCTGAC CCTCACGGGG TTCACACCCA AGAACCACGC CAAGACTGTG 
TCCGGTGGGC GGCCCCCGCT AGGCTTCCTG GGAAAGCGGA AGCCAGGCAA GCACCCCCAC 
TATGGCGGCT TCAGCCGCCA TCATCGCTGG TTGCGCATCC ACAGCGAGAC CCAGTCGCTA 
TCGCTCTCTG AGGGCATTTA TACACAGCAC CTGTCCCAGG CCTGTGGTCT GGCCCAGGAG 
CCCAAGGAGG CCAAGCGGGC CGGCCCTGCC CTTGATGATC CCAGGGGCCT AACTCTGGCC 
CACGCTGCCA GCCTTCCTGA GGAGCGGAAC AGCAGTCTCC AGGACGCGAG GCCTTCCGTC 
CGCCGCCTCT ACGTGGAGCC CTACGCAGCG GCCGTGGTGG CCCAGGACGA AAAACCATGG 
GCAAGCTTGA GCCCGCCCTC TGGAGGCCGC ATCACCCTGC CCAGCCCCAC TGGCCCCGAT 
GTGAGTCAGG AGGGCCCCAC CGCCTCTCCC ATGTCAGAAA TACTCAGCAG CATGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_011524434.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522736.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X15, mRNA.

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CloneID	OHu77170
Clone ID Related Accession (Same CDS sequence)	XM_011524434.2
Accession Version	XM_011524434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGCA AGCTTCATTG TTCACAAGAA AGCACAAAAT TACAAACCAC AGGCCGCAAG 
CAGTCAGTCT CAAGAAGCCT GAAACACCTG TTCCATTCCT CGAACAAGTT TGTGAAGACC 
TTAAAACGGG GACTCTACAT AGCCGTTATA TTTTATTACA AAGACAATAT CTTAGTCACC 
AATGAAGATC AAGTACCAAT TGTTGAAATA GATGACTCTC ACACCAGTTC TATTACACAA 
GATTTTCTGT GGTTCACGAA GCTGTCTTGT ATGTGGGAAG ATATAAGGTG GCTGAGGCAA 
AGCATACCAA TATCCTCATC CTCATCCACA GTGCTGCAAA CTCGGCAGAA GATGCTCGCA 
GCAACAGCAC AGCTACAGAA TTTACTTGGG ACACACAACT TGGGAAGAGT TTACTATGAG 
CCCATTAAAG ATCGACATGG AAACATACTC ATAGTCACCA TCAGGGAGGT GGAGATGCTT 
TATTCATTTT TTAATGGCAA ATGGATGCAG ATCTCAAAGC TGCAAAGCCA GAGAAAGTCT 
CTATCAACAC CTGAGGAGCC AACAGCTTTA GACATTCTAC TGATAACCAT CCAGGATATT 
CTATCCTATC ACAAAAGGAG TCATCAGCGT CTCTTTCCTG GATTATATCT GGGTTACCTA 
AAGCTCTGTA GCTCTGTGGA TCAAATCAAA GTTCTTGTTA CCCAAAAGTT GCCCAACATT 
CTCTGCCACG TGAAGATCCG TGAAAACAAT AATATTTCTA GAGAGGAATG GGAATGGATC 
CAAAAGCTTT CTGGCTCTGA ATCTATGGAA AGTGTGGATC ATACTTCTGA CTGCCCCATG 
CAATTGTTCT TCTACGAGCT CCAGATGGCA GTGAAAGCTC TCCTTCAGCA GATCAATATA 
CCTCTACACC AGGCAAGGAA CTTCCGCCTC TACACACAGG AGGTGTTGGA AATGGGTCAC 
AATGTGTCCT TTCTTCTCCT GCTCCCTGCC TCAGACGACG TCTGTACAGC CCCAGGACAG 
AATAATCCTT ACACCCCACA CTCAGGGTTT CTTAACCTCC CTCTTCAGAT GTTTGAACTT 
GTTCATTTTT GCAGCTACAG AGAGAAATTT ATTAGTCTGT ATTGCCGCCT TTCTGCTGTT 
GTGGAGCTGG ATTCTCTGAA CACCCAACAG TCCCTCAGGG AAGCAATCTC AGACAGCGAG 
GTTGCAGCTG CCAAACAAAG ACACCAGCAA GTTTTAGATT TCATTCAGCA AATAGATGAA 
GTCTGGCGTG AAATGAGATG GATCATGGAT GCTCTACAGT ATGCAAGATA CAAACAACCA 
GTTTCTGGCT TGCCCATCAC TAAGCTGATA GACCCCTCAG ATGAGCAGAG CCTAAAGAAG 
ATCAATTCTA CATCATCATC ACATATAGAC TGTCTTCCAT CCCCACCCCC ATCCCCAGAG 
ATGCACAGAA GAAAGACAGT GAGTGATTCA CAGCCCTGCT CTGATGAAGA AGCCTGCTCA 
GAAGTCTTCC TCCCCACCAA CAGTGACTAC GACTCCAGCG ATGCCCTGAG CCCCAGAGAC 
CTGGACCTGG TCTACCTATC ATCTCACGAC ATTGCGCAGC AGACCCTTAG CGGCCTAAGC 
GGCAGCGCCC CCGACGTCCT GCAAGTGCAC GACGTGAAAA CCCCTCTGGG GCCGGGCCAG 
GATCCCCAGG GCGAGGGCCC AAATCCCGAT CACTCATGTG CCGAGTTTCT CCATAGCCTG 
ACCCTCACGG GGTTCACACC CAAGAACCAC GCCAAGACTG TGTCCGGTGG GCGGCCCCCG 
CTAGGCTTCC TGGGAAAGCG GAAGCCAGGC AAGCACCCCC ACTATGGCGG CTTCAGCCGC 
CATCATCGCT GGTTGCGCAT CCACAGCGAG ACCCAGTCGC TATCGCTCTC TGAGGGCATT 
TATACACAGC ACCTGTCCCA GGCCTGTGGT CTGGCCCAGG AGCCCAAGGA GGCCAAGCGG 
GCCGGCCCTG CCCTTGATGA TCCCAGGGGC CTAACTCTGG CCCACGCTGC CAGCCTTCCT 
GAGGAGCGGA ACAGCAGTCT CCAGGACGCG AGGCCTTCCG TCCGCCGCCT CTACGTGGAG 
CCCTACGCAG CGGCCGTGGT GGCCCAGGAC GAAAAACCAT GGGCAAGCTT GAGCCCGCCC 
TCTGGAGGCC GCATCACCCT GCCCAGCCCC ACTGGCCCCG ATGTGAGTCA GGAGGGCCCC 
ACCGCCTCTC CCATGTCAGA AATACTCAGC AGCATGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522736.1
CDS	84..2345
Translation	MLSKLHCSQESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_011524434.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524434.2

ORF Insert Sequence:

ATGTTAAGCA AGCTTCATTG TTCACAAGAA AGCACAAAAT TACAAACCAC AGGCCGCAAG 
CAGTCAGTCT CAAGAAGCCT GAAACACCTG TTCCATTCCT CGAACAAGTT TGTGAAGACC 
TTAAAACGGG GACTCTACAT AGCCGTTATA TTTTATTACA AAGACAATAT CTTAGTCACC 
AATGAAGATC AAGTACCAAT TGTTGAAATA GATGACTCTC ACACCAGTTC TATTACACAA 
GATTTTCTGT GGTTCACGAA GCTGTCTTGT ATGTGGGAAG ATATAAGGTG GCTGAGGCAA 
AGCATACCAA TATCCTCATC CTCATCCACA GTGCTGCAAA CTCGGCAGAA GATGCTCGCA 
GCAACAGCAC AGCTACAGAA TTTACTTGGG ACACACAACT TGGGAAGAGT TTACTATGAG 
CCCATTAAAG ATCGACATGG AAACATACTC ATAGTCACCA TCAGGGAGGT GGAGATGCTT 
TATTCATTTT TTAATGGCAA ATGGATGCAG ATCTCAAAGC TGCAAAGCCA GAGAAAGTCT 
CTATCAACAC CTGAGGAGCC AACAGCTTTA GACATTCTAC TGATAACCAT CCAGGATATT 
CTATCCTATC ACAAAAGGAG TCATCAGCGT CTCTTTCCTG GATTATATCT GGGTTACCTA 
AAGCTCTGTA GCTCTGTGGA TCAAATCAAA GTTCTTGTTA CCCAAAAGTT GCCCAACATT 
CTCTGCCACG TGAAGATCCG TGAAAACAAT AATATTTCTA GAGAGGAATG GGAATGGATC 
CAAAAGCTTT CTGGCTCTGA ATCTATGGAA AGTGTGGATC ATACTTCTGA CTGCCCCATG 
CAATTGTTCT TCTACGAGCT CCAGATGGCA GTGAAAGCTC TCCTTCAGCA GATCAATATA 
CCTCTACACC AGGCAAGGAA CTTCCGCCTC TACACACAGG AGGTGTTGGA AATGGGTCAC 
AATGTGTCCT TTCTTCTCCT GCTCCCTGCC TCAGACGACG TCTGTACAGC CCCAGGACAG 
AATAATCCTT ACACCCCACA CTCAGGGTTT CTTAACCTCC CTCTTCAGAT GTTTGAACTT 
GTTCATTTTT GCAGCTACAG AGAGAAATTT ATTAGTCTGT ATTGCCGCCT TTCTGCTGTT 
GTGGAGCTGG ATTCTCTGAA CACCCAACAG TCCCTCAGGG AAGCAATCTC AGACAGCGAG 
GTTGCAGCTG CCAAACAAAG ACACCAGCAA GTTTTAGATT TCATTCAGCA AATAGATGAA 
GTCTGGCGTG AAATGAGATG GATCATGGAT GCTCTACAGT ATGCAAGATA CAAACAACCA 
GTTTCTGGCT TGCCCATCAC TAAGCTGATA GACCCCTCAG ATGAGCAGAG CCTAAAGAAG 
ATCAATTCTA CATCATCATC ACATATAGAC TGTCTTCCAT CCCCACCCCC ATCCCCAGAG 
ATGCACAGAA GAAAGACAGT GAGTGATTCA CAGCCCTGCT CTGATGAAGA AGCCTGCTCA 
GAAGTCTTCC TCCCCACCAA CAGTGACTAC GACTCCAGCG ATGCCCTGAG CCCCAGAGAC 
CTGGACCTGG TCTACCTATC ATCTCACGAC ATTGCGCAGC AGACCCTTAG CGGCCTAAGC 
GGCAGCGCCC CCGACGTCCT GCAAGTGCAC GACGTGAAAA CCCCTCTGGG GCCGGGCCAG 
GATCCCCAGG GCGAGGGCCC AAATCCCGAT CACTCATGTG CCGAGTTTCT CCATAGCCTG 
ACCCTCACGG GGTTCACACC CAAGAACCAC GCCAAGACTG TGTCCGGTGG GCGGCCCCCG 
CTAGGCTTCC TGGGAAAGCG GAAGCCAGGC AAGCACCCCC ACTATGGCGG CTTCAGCCGC 
CATCATCGCT GGTTGCGCAT CCACAGCGAG ACCCAGTCGC TATCGCTCTC TGAGGGCATT 
TATACACAGC ACCTGTCCCA GGCCTGTGGT CTGGCCCAGG AGCCCAAGGA GGCCAAGCGG 
GCCGGCCCTG CCCTTGATGA TCCCAGGGGC CTAACTCTGG CCCACGCTGC CAGCCTTCCT 
GAGGAGCGGA ACAGCAGTCT CCAGGACGCG AGGCCTTCCG TCCGCCGCCT CTACGTGGAG 
CCCTACGCAG CGGCCGTGGT GGCCCAGGAC GAAAAACCAT GGGCAAGCTT GAGCCCGCCC 
TCTGGAGGCC GCATCACCCT GCCCAGCCCC ACTGGCCCCG ATGTGAGTCA GGAGGGCCCC 
ACCGCCTCTC CCATGTCAGA AATACTCAGC AGCATGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024270.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879759.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu86639
Clone ID Related Accession (Same CDS sequence)	XM_017024270.1 , XM_017024270.2
Accession Version	XM_017024270.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGGTATAGTA 
GCTTGTTTCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879759.1
CDS	188..2542
Translation	MKNGKEDNGMESTGPTLWLSAMDHSVIWRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELGIVACFT

Target ORF information:

RefSeq Version	XM_017024270.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024270.1

ORF Insert Sequence:

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGGTATAGTA 
GCTTGTTTCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024270.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879759.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X14, mRNA.

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CloneID	OHu86639
Clone ID Related Accession (Same CDS sequence)	XM_017024270.1 , XM_017024270.2
Accession Version	XM_017024270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGGTATAGTA 
GCTTGTTTCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879759.1
CDS	188..2542
Translation	MKNGKEDNGMESTGPTLWLSAMDHSVIWRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELGIVACFT

Target ORF information:

RefSeq Version	XM_017024270.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024270.2

ORF Insert Sequence:

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGGTATAGTA 
GCTTGTTTCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( NM_001370326.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357255.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108547
Clone ID Related Accession (Same CDS sequence)	NM_001370326.1
Accession Version	NM_001370326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC006925.6, AC006600.4 and AC015724.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.228640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2151119 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END##

ATGAATGAGA AGAGGCTACT CTTTAAAGAC AGGCATTTTA CTTGCAGCAA AATAATAGGA 
AGGAGATTCG CTTGCTTTGC ACAGAGGCTG AGCCACAGGA GAAAGCAAAG CCAATGTGAT 
TTATTGAATG AAAGCACTGG ACAATTACCA ACAACTTGTT CCTCTGCTGC CTCGAACAGC 
ATAAACTGGA ATTGTCGTGT GAAAATGACG CAACAAATGC AAAATTTACA TCTCTGTCAG 
TCAAAAAAAC ATAGTGCTCC CTCATCTCCC AACGCAGCCA AACGCCTGTA CAGGAACCTC 
TCTGAGAAAC TGAAAGGGAG CCACTCTTCC TTCGATGAGG CCTATTTTAG GACAAGAACT 
GATCGGCTGA GTCTCAGGAA GACCTCGGTG AATTTCCAGG GCAATGAAGC CATGTTTGAG 
GCAGTCGAAC AGCAGGACAT GGATGCTGTG CAGATCCTCC TGTATCAGTA CACACCAGAA 
GAACTTGACC TCAACACACC TAACAGCGAG GGCTTGACAC CCCTGGATAT TGCCATCATG 
ACCAACAATG TGCCCATTGC AAGGATTCTT CTGAGGACAG GGGCCCGAGA AAGTCCACAC 
TTTGTCAGCC TGGAAAGCCG AGCAATGCAC CTCAACACAC TGGTCCAGGA AGCCCAGGAG 
AGGGTGAGTG AACTGTCTGC CCAGGTGGAG AATGAAGGAT TCACTCTGGA CAACACAGAG 
AAAGAGAAGC AGCTGAAAGC TTGGGAGTGG AGGTATCGGC TCTACAGACG CATGAAAACA 
GGCTTTGAGC ATGCCAGAGC CCCTGAGATG CCAACCAATG TCTGTCTCAT GGTAACCAGC 
AGCACATCAC TCACTGTCAG CTTCCAAGAG CCTCTTAGCG TCAATGCAGC TGTAGTAACC 
AGGTATAAAG TGGAATGGAG TATGTCCGAA GACTTTTCTC CTTTGGCTGG AGAAATCATC 
ATGGATAATC TGCAGACTCT GAGATGCACA ATCACAGGAC TTACAATGGG CCAACAGTAT 
TTTGTTCAAG TCTCGGCTTA CAATATGAAA GGATGGGGAC CTGCTCAGAC CACGACACCG 
GCATGTGCCT CTCCTTCTAA CTGGAAAGAC TATGACGACA GAGAGCCCAG ACACAAGGGA 
CAGAGTGAAG TTTTGGAAGG TCTGCTGCAG CAGGTCCGAG CCCTTCATCA GCATTACAGT 
TGCCGGGAAA GCACAAAATT ACAAACCACA GGCCGCAAGC AGTCAGTCTC AAGAAGCCTG 
AAACACCTGT TCCATTCCTC GAACAAGTTT GTGAAGACCT TAAAACGGGG ACTCTACATA 
GCCGTTATAT TTTATTACAA AGACAATATC TTAGTCACCA ATGAAGATCA AGTACCAATT 
GTTGAAATAG ATGACTCTCA CACCAGTTCT ATTACACAAG ATTTTCTGTG GTTCACGAAG 
CTGTCTTGTA TGTGGGAAGA TATAAGGTGG CTGAGGCAAA GCATACCAAT ATCCTCATCC 
TCATCCACAG TGCTGCAAAC TCGGCAGAAG ATGCTCGCAG CAACAGCACA GCTACAGAAT 
TTACTTGGGA CACACAACTT GGGAAGAGTT TACTATGAGC CCATTAAAGA TCGACATGGA 
AACATACTCA TAGTCACCAT CAGGGAGGTG GAGATGCTTT ATTCATTTTT TAATGGCAAA 
TGGATGCAGA TCTCAAAGCT GCAAAGCCAG AGAAAGTCTC TATCAACACC TGAGGAGCCA 
ACAGCTTTAG ACATTCTACT GATAACCATC CAGGATATTC TATCCTATCA CAAAAGGAGT 
CATCAGCGTC TCTTTCCTGG ATTATATCTG GGTTACCTAA AGCTCTGTAG CTCTGTGGAT 
CAAATCAAAG TTCTTGTTAC CCAAAAGTTG CCCAACATTC TCTGCCACGT GAAGATCCGT 
GAAAACAATA ATATTTCTAG AGAGGAATGG GAATGGATCC AAAAGCTTTC TGGCTCTGAA 
TCTATGGAAA GTGTGGATCA TACTTCTGAC TGCCCCATGC AATTGTTCTT CTACGAGCTC 
CAGATGGCAG TGAAAGCTCT CCTTCAGCAG ATCAATATAC CTCTACACCA GGCAAGGAAC 
TTCCGCCTCT ACACACAGGA GGTGTTGGAA ATGGGTCACA ATGTGTCCTT TCTTCTCCTG 
CTCCCTGCCT CAGACGACGT CTGTACAGCC CCAGGACAGA ATAATCCTTA CACCCCACAC 
TCAGGGTTTC TTAACCTCCC TCTTCAGATG TTTGAACTTG TTCATTTTTG CAGCTACAGA 
GAGAAATTTA TTAGTCTGTA TTGCCGCCTT TCTGCTGTTG TGGAGCTGGA TTCTCTGAAC 
ACCCAACAGT CCCTCAGGGA AGCAATCTCA GACAGCGAGG TTGCAGCTGC CAAACAAAGA 
CACCAGCAAG TTTTAGATTT CATTCAGCAA ATAGATGAAG TCTGGCGTGA AATGAGATGG 
ATCATGGATG CTCTACAGTA TGCAAGATAC AAACAACCAG TTTCTGGCTT GCCCATCACT 
AAGCTGATAG ACCCCTCAGA TGAGCAGAGC CTAAAGAAGA TCAATTCTAC ATCATCATCA 
CATATAGACT GTCTTCCATC CCCACCCCCA TCCCCAGAGA TGCACAGAAG AAAGACAGTG 
AGTGATTCAC AGCCCTGCTC TGATGAAGAA GCCTGCTCAG AAGTCTTCCT CCCCACCAAC 
AGTGACTACG ACTCCAGCGA TGCCCTGAGC CCCAGAGACC TGGACCTGGT CTACCTATCA 
TCTCACGACA TTGCGCAGCA GACCCTTAGC GGCCTAAGCG GCAGCGCCCC CGACGTCCTG 
CAAGTGCACG ACGTGAAAAC CCCTCTGGGG CCGGGCCAGG ATCCCCAGGG CGAGGGCCCA 
AATCCCGATC ACTCATGTGC CGAGTTTCTC CATAGCCTGA CCCTCACGGG GTTCACACCC 
AAGAACCACG CCAAGACTGT GTCCGGTGGG CGGCCCCCGC TAGGCTTCCT GGGAAAGCGG 
AAGCCAGGCA AGCACCCCCA CTATGGCGGC TTCAGCCGCC ATCATCGCTG GTTGCGCATC 
CACAGCGAGA CCCAGTCGCT ATCGCTCTCT GAGGGCATTT ATACACAGCA CCTGTCCCAG 
GCCTGTGGTC TGGCCCAGGA GCCCAAGGAG GCCAAGCGGG CCGGCCCTGC CCTTGATGAT 
CCCAGGGGCC TAACTCTGGC CCACGCTGCC AGCCTTCCTG AGGAGCGGAA CAGCAGTCTC 
CAGGACGCGA GGCCTTCCGT CCGCCGCCTC TACGTGGAGC CCTACGCAGC GGCCGTGGTG 
GCCCAGGACG AAAAACCATG GGCAAGCTTG AGCCCGCCCT CTGGAGGCCG CATCACCCTG 
CCCAGCCCCA CTGGCCCCGA TGTGAGTCAG GAGGGCCCCA CCGCCTCTCC CATGTCAGAA 
ATACTCAGCA GCATGCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357255.1
CDS	127..3567
Translation	MNEKRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	NM_001370326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370326.1

ORF Insert Sequence:

ATGAATGAGA AGAGGCTACT CTTTAAAGAC AGGCATTTTA CTTGCAGCAA AATAATAGGA 
AGGAGATTCG CTTGCTTTGC ACAGAGGCTG AGCCACAGGA GAAAGCAAAG CCAATGTGAT 
TTATTGAATG AAAGCACTGG ACAATTACCA ACAACTTGTT CCTCTGCTGC CTCGAACAGC 
ATAAACTGGA ATTGTCGTGT GAAAATGACG CAACAAATGC AAAATTTACA TCTCTGTCAG 
TCAAAAAAAC ATAGTGCTCC CTCATCTCCC AACGCAGCCA AACGCCTGTA CAGGAACCTC 
TCTGAGAAAC TGAAAGGGAG CCACTCTTCC TTCGATGAGG CCTATTTTAG GACAAGAACT 
GATCGGCTGA GTCTCAGGAA GACCTCGGTG AATTTCCAGG GCAATGAAGC CATGTTTGAG 
GCAGTCGAAC AGCAGGACAT GGATGCTGTG CAGATCCTCC TGTATCAGTA CACACCAGAA 
GAACTTGACC TCAACACACC TAACAGCGAG GGCTTGACAC CCCTGGATAT TGCCATCATG 
ACCAACAATG TGCCCATTGC AAGGATTCTT CTGAGGACAG GGGCCCGAGA AAGTCCACAC 
TTTGTCAGCC TGGAAAGCCG AGCAATGCAC CTCAACACAC TGGTCCAGGA AGCCCAGGAG 
AGGGTGAGTG AACTGTCTGC CCAGGTGGAG AATGAAGGAT TCACTCTGGA CAACACAGAG 
AAAGAGAAGC AGCTGAAAGC TTGGGAGTGG AGGTATCGGC TCTACAGACG CATGAAAACA 
GGCTTTGAGC ATGCCAGAGC CCCTGAGATG CCAACCAATG TCTGTCTCAT GGTAACCAGC 
AGCACATCAC TCACTGTCAG CTTCCAAGAG CCTCTTAGCG TCAATGCAGC TGTAGTAACC 
AGGTATAAAG TGGAATGGAG TATGTCCGAA GACTTTTCTC CTTTGGCTGG AGAAATCATC 
ATGGATAATC TGCAGACTCT GAGATGCACA ATCACAGGAC TTACAATGGG CCAACAGTAT 
TTTGTTCAAG TCTCGGCTTA CAATATGAAA GGATGGGGAC CTGCTCAGAC CACGACACCG 
GCATGTGCCT CTCCTTCTAA CTGGAAAGAC TATGACGACA GAGAGCCCAG ACACAAGGGA 
CAGAGTGAAG TTTTGGAAGG TCTGCTGCAG CAGGTCCGAG CCCTTCATCA GCATTACAGT 
TGCCGGGAAA GCACAAAATT ACAAACCACA GGCCGCAAGC AGTCAGTCTC AAGAAGCCTG 
AAACACCTGT TCCATTCCTC GAACAAGTTT GTGAAGACCT TAAAACGGGG ACTCTACATA 
GCCGTTATAT TTTATTACAA AGACAATATC TTAGTCACCA ATGAAGATCA AGTACCAATT 
GTTGAAATAG ATGACTCTCA CACCAGTTCT ATTACACAAG ATTTTCTGTG GTTCACGAAG 
CTGTCTTGTA TGTGGGAAGA TATAAGGTGG CTGAGGCAAA GCATACCAAT ATCCTCATCC 
TCATCCACAG TGCTGCAAAC TCGGCAGAAG ATGCTCGCAG CAACAGCACA GCTACAGAAT 
TTACTTGGGA CACACAACTT GGGAAGAGTT TACTATGAGC CCATTAAAGA TCGACATGGA 
AACATACTCA TAGTCACCAT CAGGGAGGTG GAGATGCTTT ATTCATTTTT TAATGGCAAA 
TGGATGCAGA TCTCAAAGCT GCAAAGCCAG AGAAAGTCTC TATCAACACC TGAGGAGCCA 
ACAGCTTTAG ACATTCTACT GATAACCATC CAGGATATTC TATCCTATCA CAAAAGGAGT 
CATCAGCGTC TCTTTCCTGG ATTATATCTG GGTTACCTAA AGCTCTGTAG CTCTGTGGAT 
CAAATCAAAG TTCTTGTTAC CCAAAAGTTG CCCAACATTC TCTGCCACGT GAAGATCCGT 
GAAAACAATA ATATTTCTAG AGAGGAATGG GAATGGATCC AAAAGCTTTC TGGCTCTGAA 
TCTATGGAAA GTGTGGATCA TACTTCTGAC TGCCCCATGC AATTGTTCTT CTACGAGCTC 
CAGATGGCAG TGAAAGCTCT CCTTCAGCAG ATCAATATAC CTCTACACCA GGCAAGGAAC 
TTCCGCCTCT ACACACAGGA GGTGTTGGAA ATGGGTCACA ATGTGTCCTT TCTTCTCCTG 
CTCCCTGCCT CAGACGACGT CTGTACAGCC CCAGGACAGA ATAATCCTTA CACCCCACAC 
TCAGGGTTTC TTAACCTCCC TCTTCAGATG TTTGAACTTG TTCATTTTTG CAGCTACAGA 
GAGAAATTTA TTAGTCTGTA TTGCCGCCTT TCTGCTGTTG TGGAGCTGGA TTCTCTGAAC 
ACCCAACAGT CCCTCAGGGA AGCAATCTCA GACAGCGAGG TTGCAGCTGC CAAACAAAGA 
CACCAGCAAG TTTTAGATTT CATTCAGCAA ATAGATGAAG TCTGGCGTGA AATGAGATGG 
ATCATGGATG CTCTACAGTA TGCAAGATAC AAACAACCAG TTTCTGGCTT GCCCATCACT 
AAGCTGATAG ACCCCTCAGA TGAGCAGAGC CTAAAGAAGA TCAATTCTAC ATCATCATCA 
CATATAGACT GTCTTCCATC CCCACCCCCA TCCCCAGAGA TGCACAGAAG AAAGACAGTG 
AGTGATTCAC AGCCCTGCTC TGATGAAGAA GCCTGCTCAG AAGTCTTCCT CCCCACCAAC 
AGTGACTACG ACTCCAGCGA TGCCCTGAGC CCCAGAGACC TGGACCTGGT CTACCTATCA 
TCTCACGACA TTGCGCAGCA GACCCTTAGC GGCCTAAGCG GCAGCGCCCC CGACGTCCTG 
CAAGTGCACG ACGTGAAAAC CCCTCTGGGG CCGGGCCAGG ATCCCCAGGG CGAGGGCCCA 
AATCCCGATC ACTCATGTGC CGAGTTTCTC CATAGCCTGA CCCTCACGGG GTTCACACCC 
AAGAACCACG CCAAGACTGT GTCCGGTGGG CGGCCCCCGC TAGGCTTCCT GGGAAAGCGG 
AAGCCAGGCA AGCACCCCCA CTATGGCGGC TTCAGCCGCC ATCATCGCTG GTTGCGCATC 
CACAGCGAGA CCCAGTCGCT ATCGCTCTCT GAGGGCATTT ATACACAGCA CCTGTCCCAG 
GCCTGTGGTC TGGCCCAGGA GCCCAAGGAG GCCAAGCGGG CCGGCCCTGC CCTTGATGAT 
CCCAGGGGCC TAACTCTGGC CCACGCTGCC AGCCTTCCTG AGGAGCGGAA CAGCAGTCTC 
CAGGACGCGA GGCCTTCCGT CCGCCGCCTC TACGTGGAGC CCTACGCAGC GGCCGTGGTG 
GCCCAGGACG AAAAACCATG GGCAAGCTTG AGCCCGCCCT CTGGAGGCCG CATCACCCTG 
CCCAGCCCCA CTGGCCCCGA TGTGAGTCAG GAGGGCCCCA CCGCCTCTCC CATGTCAGAA 
ATACTCAGCA GCATGCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024263.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879752.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86636
Clone ID Related Accession (Same CDS sequence)	XM_017024263.1 , XM_017024264.1 , XM_017024265.2 , XM_017024265.1
Accession Version	XM_017024263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879752.1
CDS	190..3702
Translation	MKNGKEDNGMESTGPTLWLSAMDHSVIWRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024263.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024263.1

ORF Insert Sequence:

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024264.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879753.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86636
Clone ID Related Accession (Same CDS sequence)	XM_017024263.1 , XM_017024264.1 , XM_017024265.2 , XM_017024265.1
Accession Version	XM_017024264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879753.1
CDS	1175..4687
Translation	MKNGKEDNGMESTGPTLWLSAMDHSVIWRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024264.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024264.1

ORF Insert Sequence:

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024265.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879754.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu86636
Clone ID Related Accession (Same CDS sequence)	XM_017024263.1 , XM_017024264.1 , XM_017024265.2 , XM_017024265.1
Accession Version	XM_017024265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879754.1
CDS	385..3897
Translation	MKNGKEDNGMESTGPTLWLSAMDHSVIWRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024265.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024265.2

ORF Insert Sequence:

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024265.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879754.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu86636
Clone ID Related Accession (Same CDS sequence)	XM_017024263.1 , XM_017024264.1 , XM_017024265.2 , XM_017024265.1
Accession Version	XM_017024265.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879754.1
CDS	384..3896
Translation	MKNGKEDNGMESTGPTLWLSAMDHSVIWRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024265.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024265.1

ORF Insert Sequence:

ATGAAGAACG GCAAAGAAGA CAATGGGATG GAAAGTACCG GGCCCACACT GTGGTTATCA 
GCTATGGATC ACAGTGTTAT CTGGAGGCTA CTCTTTAAAG ACAGGCATTT TACTTGCAGC 
AAAATAATAG GAAGGAGATT CGCTTGCTTT GCACAGAGGC TGAGCCACAG GAGAAAGCAA 
AGCCAATGTG ATTTATTGAA TGAAAGCACT GGACAATTAC CAACAACTTG TTCCTCTGCT 
GCCTCGAACA GCATAAACTG GAATTGTCGT GTGAAAATGA CGCAACAAAT GCAAAATTTA 
CATCTCTGTC AGTCAAAAAA ACATAGTGCT CCCTCATCTC CCAACGCAGC CAAACGCCTG 
TACAGGAACC TCTCTGAGAA ACTGAAAGGG AGCCACTCTT CCTTCGATGA GGCCTATTTT 
AGGACAAGAA CTGATCGGCT GAGTCTCAGG AAGACCTCGG TGAATTTCCA GGGCAATGAA 
GCCATGTTTG AGGCAGTCGA ACAGCAGGAC ATGGATGCTG TGCAGATCCT CCTGTATCAG 
TACACACCAG AAGAACTTGA CCTCAACACA CCTAACAGCG AGGGCTTGAC ACCCCTGGAT 
ATTGCCATCA TGACCAACAA TGTGCCCATT GCAAGGATTC TTCTGAGGAC AGGGGCCCGA 
GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC CGAGCAATGC ACCTCAACAC ACTGGTCCAG 
GAAGCCCAGG AGAGGGTGAG TGAACTGTCT GCCCAGGTGG AGAATGAAGG ATTCACTCTG 
GACAACACAG AGAAAGAGAA GCAGCTGAAA GCTTGGGAGT GGAGGTATCG GCTCTACAGA 
CGCATGAAAA CAGGCTTTGA GCATGCCAGA GCCCCTGAGA TGCCAACCAA TGTCTGTCTC 
ATGGTAACCA GCAGCACATC ACTCACTGTC AGCTTCCAAG AGCCTCTTAG CGTCAATGCA 
GCTGTAGTAA CCAGGTATAA AGTGGAATGG AGTATGTCCG AAGACTTTTC TCCTTTGGCT 
GGAGAAATCA TCATGGATAA TCTGCAGACT CTGAGATGCA CAATCACAGG ACTTACAATG 
GGCCAACAGT ATTTTGTTCA AGTCTCGGCT TACAATATGA AAGGATGGGG ACCTGCTCAG 
ACCACGACAC CGGCATGTGC CTCTCCTTCT AACTGGAAAG ACTATGACGA CAGAGAGCCC 
AGACACAAGG GACAGAGTGA AGTTTTGGAA GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT 
CAGCATTACA GTTGCCGGGA AAGCACAAAA TTACAAACCA CAGGCCGCAA GCAGTCAGTC 
TCAAGAAGCC TGAAACACCT GTTCCATTCC TCGAACAAGT TTGTGAAGAC CTTAAAACGG 
GGACTCTACA TAGCCGTTAT ATTTTATTAC AAAGACAATA TCTTAGTCAC CAATGAAGAT 
CAAGTACCAA TTGTTGAAAT AGATGACTCT CACACCAGTT CTATTACACA AGATTTTCTG 
TGGTTCACGA AGCTGTCTTG TATGTGGGAA GATATAAGGT GGCTGAGGCA AAGCATACCA 
ATATCCTCAT CCTCATCCAC AGTGCTGCAA ACTCGGCAGA AGATGCTCGC AGCAACAGCA 
CAGCTACAGA ATTTACTTGG GACACACAAC TTGGGAAGAG TTTACTATGA GCCCATTAAA 
GATCGACATG GAAACATACT CATAGTCACC ATCAGGGAGG TGGAGATGCT TTATTCATTT 
TTTAATGGCA AATGGATGCA GATCTCAAAG CTGCAAAGCC AGAGAAAGTC TCTATCAACA 
CCTGAGGAGC CAACAGCTTT AGACATTCTA CTGATAACCA TCCAGGATAT TCTATCCTAT 
CACAAAAGGA GTCATCAGCG TCTCTTTCCT GGATTATATC TGGGTTACCT AAAGCTCTGT 
AGCTCTGTGG ATCAAATCAA AGTTCTTGTT ACCCAAAAGT TGCCCAACAT TCTCTGCCAC 
GTGAAGATCC GTGAAAACAA TAATATTTCT AGAGAGGAAT GGGAATGGAT CCAAAAGCTT 
TCTGGCTCTG AATCTATGGA AAGTGTGGAT CATACTTCTG ACTGCCCCAT GCAATTGTTC 
TTCTACGAGC TCCAGATGGC AGTGAAAGCT CTCCTTCAGC AGATCAATAT ACCTCTACAC 
CAGGCAAGGA ACTTCCGCCT CTACACACAG GAGGTGTTGG AAATGGGTCA CAATGTGTCC 
TTTCTTCTCC TGCTCCCTGC CTCAGACGAC GTCTGTACAG CCCCAGGACA GAATAATCCT 
TACACCCCAC ACTCAGGGTT TCTTAACCTC CCTCTTCAGA TGTTTGAACT TGTTCATTTT 
TGCAGCTACA GAGAGAAATT TATTAGTCTG TATTGCCGCC TTTCTGCTGT TGTGGAGCTG 
GATTCTCTGA ACACCCAACA GTCCCTCAGG GAAGCAATCT CAGACAGCGA GGTTGCAGCT 
GCCAAACAAA GACACCAGCA AGTTTTAGAT TTCATTCAGC AAATAGATGA AGTCTGGCGT 
GAAATGAGAT GGATCATGGA TGCTCTACAG TATGCAAGAT ACAAACAACC AGTTTCTGGC 
TTGCCCATCA CTAAGCTGAT AGACCCCTCA GATGAGCAGA GCCTAAAGAA GATCAATTCT 
ACATCATCAT CACATATAGA CTGTCTTCCA TCCCCACCCC CATCCCCAGA GATGCACAGA 
AGAAAGACAG TGAGTGATTC ACAGCCCTGC TCTGATGAAG AAGCCTGCTC AGAAGTCTTC 
CTCCCCACCA ACAGTGACTA CGACTCCAGC GATGCCCTGA GCCCCAGAGA CCTGGACCTG 
GTCTACCTAT CATCTCACGA CATTGCGCAG CAGACCCTTA GCGGCCTAAG CGGCAGCGCC 
CCCGACGTCC TGCAAGTGCA CGACGTGAAA ACCCCTCTGG GGCCGGGCCA GGATCCCCAG 
GGCGAGGGCC CAAATCCCGA TCACTCATGT GCCGAGTTTC TCCATAGCCT GACCCTCACG 
GGGTTCACAC CCAAGAACCA CGCCAAGACT GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC 
CTGGGAAAGC GGAAGCCAGG CAAGCACCCC CACTATGGCG GCTTCAGCCG CCATCATCGC 
TGGTTGCGCA TCCACAGCGA GACCCAGTCG CTATCGCTCT CTGAGGGCAT TTATACACAG 
CACCTGTCCC AGGCCTGTGG TCTGGCCCAG GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT 
GCCCTTGATG ATCCCAGGGG CCTAACTCTG GCCCACGCTG CCAGCCTTCC TGAGGAGCGG 
AACAGCAGTC TCCAGGACGC GAGGCCTTCC GTCCGCCGCC TCTACGTGGA GCCCTACGCA 
GCGGCCGTGG TGGCCCAGGA CGAAAAACCA TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC 
CGCATCACCC TGCCCAGCCC CACTGGCCCC GATGTGAGTC AGGAGGGCCC CACCGCCTCT 
CCCATGTCAG AAATACTCAG CAGCATGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024266.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879755.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X4, mRNA.

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CloneID	OHu86637
Clone ID Related Accession (Same CDS sequence)	XM_017024266.1
Accession Version	XM_017024266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTACT GTGATCCGAG GAGGGTAACC TGCTGGCCAT GCAGATCTTC TGAGAGGCTA 
CTCTTTAAAG ACAGGCATTT TACTTGCAGC AAAATAATAG GAAGGAGATT CGCTTGCTTT 
GCACAGAGGC TGAGCCACAG GAGAAAGCAA AGCCAATGTG ATTTATTGAA TGAAAGCACT 
GGACAATTAC CAACAACTTG TTCCTCTGCT GCCTCGAACA GCATAAACTG GAATTGTCGT 
GTGAAAATGA CGCAACAAAT GCAAAATTTA CATCTCTGTC AGTCAAAAAA ACATAGTGCT 
CCCTCATCTC CCAACGCAGC CAAACGCCTG TACAGGAACC TCTCTGAGAA ACTGAAAGGG 
AGCCACTCTT CCTTCGATGA GGCCTATTTT AGGACAAGAA CTGATCGGCT GAGTCTCAGG 
AAGACCTCGG TGAATTTCCA GGGCAATGAA GCCATGTTTG AGGCAGTCGA ACAGCAGGAC 
ATGGATGCTG TGCAGATCCT CCTGTATCAG TACACACCAG AAGAACTTGA CCTCAACACA 
CCTAACAGCG AGGGCTTGAC ACCCCTGGAT ATTGCCATCA TGACCAACAA TGTGCCCATT 
GCAAGGATTC TTCTGAGGAC AGGGGCCCGA GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC 
CGAGCAATGC ACCTCAACAC ACTGGTCCAG GAAGCCCAGG AGAGGGTGAG TGAACTGTCT 
GCCCAGGTGG AGAATGAAGG ATTCACTCTG GACAACACAG AGAAAGAGAA GCAGCTGAAA 
GCTTGGGAGT GGAGGTATCG GCTCTACAGA CGCATGAAAA CAGGCTTTGA GCATGCCAGA 
GCCCCTGAGA TGCCAACCAA TGTCTGTCTC ATGGTAACCA GCAGCACATC ACTCACTGTC 
AGCTTCCAAG AGCCTCTTAG CGTCAATGCA GCTGTAGTAA CCAGGTATAA AGTGGAATGG 
AGTATGTCCG AAGACTTTTC TCCTTTGGCT GGAGAAATCA TCATGGATAA TCTGCAGACT 
CTGAGATGCA CAATCACAGG ACTTACAATG GGCCAACAGT ATTTTGTTCA AGTCTCGGCT 
TACAATATGA AAGGATGGGG ACCTGCTCAG ACCACGACAC CGGCATGTGC CTCTCCTTCT 
AACTGGAAAG ACTATGACGA CAGAGAGCCC AGACACAAGG GACAGAGTGA AGTTTTGGAA 
GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT CAGCATTACA GTTGCCGGGA AAGCACAAAA 
TTACAAACCA CAGGCCGCAA GCAGTCAGTC TCAAGAAGCC TGAAACACCT GTTCCATTCC 
TCGAACAAGT TTGTGAAGAC CTTAAAACGG GGACTCTACA TAGCCGTTAT ATTTTATTAC 
AAAGACAATA TCTTAGTCAC CAATGAAGAT CAAGTACCAA TTGTTGAAAT AGATGACTCT 
CACACCAGTT CTATTACACA AGATTTTCTG TGGTTCACGA AGCTGTCTTG TATGTGGGAA 
GATATAAGGT GGCTGAGGCA AAGCATACCA ATATCCTCAT CCTCATCCAC AGTGCTGCAA 
ACTCGGCAGA AGATGCTCGC AGCAACAGCA CAGCTACAGA ATTTACTTGG GACACACAAC 
TTGGGAAGAG TTTACTATGA GCCCATTAAA GATCGACATG GAAACATACT CATAGTCACC 
ATCAGGGAGG TGGAGATGCT TTATTCATTT TTTAATGGCA AATGGATGCA GATCTCAAAG 
CTGCAAAGCC AGAGAAAGTC TCTATCAACA CCTGAGGAGC CAACAGCTTT AGACATTCTA 
CTGATAACCA TCCAGGATAT TCTATCCTAT CACAAAAGGA GTCATCAGCG TCTCTTTCCT 
GGATTATATC TGGGTTACCT AAAGCTCTGT AGCTCTGTGG ATCAAATCAA AGTTCTTGTT 
ACCCAAAAGT TGCCCAACAT TCTCTGCCAC GTGAAGATCC GTGAAAACAA TAATATTTCT 
AGAGAGGAAT GGGAATGGAT CCAAAAGCTT TCTGGCTCTG AATCTATGGA AAGTGTGGAT 
CATACTTCTG ACTGCCCCAT GCAATTGTTC TTCTACGAGC TCCAGATGGC AGTGAAAGCT 
CTCCTTCAGC AGATCAATAT ACCTCTACAC CAGGCAAGGA ACTTCCGCCT CTACACACAG 
GAGGTGTTGG AAATGGGTCA CAATGTGTCC TTTCTTCTCC TGCTCCCTGC CTCAGACGAC 
GTCTGTACAG CCCCAGGACA GAATAATCCT TACACCCCAC ACTCAGGGTT TCTTAACCTC 
CCTCTTCAGA TGTTTGAACT TGTTCATTTT TGCAGCTACA GAGAGAAATT TATTAGTCTG 
TATTGCCGCC TTTCTGCTGT TGTGGAGCTG GATTCTCTGA ACACCCAACA GTCCCTCAGG 
GAAGCAATCT CAGACAGCGA GGTTGCAGCT GCCAAACAAA GACACCAGCA AGTTTTAGAT 
TTCATTCAGC AAATAGATGA AGTCTGGCGT GAAATGAGAT GGATCATGGA TGCTCTACAG 
TATGCAAGAT ACAAACAACC AGTTTCTGGC TTGCCCATCA CTAAGCTGAT AGACCCCTCA 
GATGAGCAGA GCCTAAAGAA GATCAATTCT ACATCATCAT CACATATAGA CTGTCTTCCA 
TCCCCACCCC CATCCCCAGA GATGCACAGA AGAAAGACAG TGAGTGATTC ACAGCCCTGC 
TCTGATGAAG AAGCCTGCTC AGAAGTCTTC CTCCCCACCA ACAGTGACTA CGACTCCAGC 
GATGCCCTGA GCCCCAGAGA CCTGGACCTG GTCTACCTAT CATCTCACGA CATTGCGCAG 
CAGACCCTTA GCGGCCTAAG CGGCAGCGCC CCCGACGTCC TGCAAGTGCA CGACGTGAAA 
ACCCCTCTGG GGCCGGGCCA GGATCCCCAG GGCGAGGGCC CAAATCCCGA TCACTCATGT 
GCCGAGTTTC TCCATAGCCT GACCCTCACG GGGTTCACAC CCAAGAACCA CGCCAAGACT 
GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC CTGGGAAAGC GGAAGCCAGG CAAGCACCCC 
CACTATGGCG GCTTCAGCCG CCATCATCGC TGGTTGCGCA TCCACAGCGA GACCCAGTCG 
CTATCGCTCT CTGAGGGCAT TTATACACAG CACCTGTCCC AGGCCTGTGG TCTGGCCCAG 
GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT GCCCTTGATG ATCCCAGGGG CCTAACTCTG 
GCCCACGCTG CCAGCCTTCC TGAGGAGCGG AACAGCAGTC TCCAGGACGC GAGGCCTTCC 
GTCCGCCGCC TCTACGTGGA GCCCTACGCA GCGGCCGTGG TGGCCCAGGA CGAAAAACCA 
TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC CGCATCACCC TGCCCAGCCC CACTGGCCCC 
GATGTGAGTC AGGAGGGCCC CACCGCCTCT CCCATGTCAG AAATACTCAG CAGCATGCTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879755.1
CDS	175..3657
Translation	MSYCDPRRVTCWPCRSSERLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024266.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024266.1

ORF Insert Sequence:

ATGTCCTACT GTGATCCGAG GAGGGTAACC TGCTGGCCAT GCAGATCTTC TGAGAGGCTA 
CTCTTTAAAG ACAGGCATTT TACTTGCAGC AAAATAATAG GAAGGAGATT CGCTTGCTTT 
GCACAGAGGC TGAGCCACAG GAGAAAGCAA AGCCAATGTG ATTTATTGAA TGAAAGCACT 
GGACAATTAC CAACAACTTG TTCCTCTGCT GCCTCGAACA GCATAAACTG GAATTGTCGT 
GTGAAAATGA CGCAACAAAT GCAAAATTTA CATCTCTGTC AGTCAAAAAA ACATAGTGCT 
CCCTCATCTC CCAACGCAGC CAAACGCCTG TACAGGAACC TCTCTGAGAA ACTGAAAGGG 
AGCCACTCTT CCTTCGATGA GGCCTATTTT AGGACAAGAA CTGATCGGCT GAGTCTCAGG 
AAGACCTCGG TGAATTTCCA GGGCAATGAA GCCATGTTTG AGGCAGTCGA ACAGCAGGAC 
ATGGATGCTG TGCAGATCCT CCTGTATCAG TACACACCAG AAGAACTTGA CCTCAACACA 
CCTAACAGCG AGGGCTTGAC ACCCCTGGAT ATTGCCATCA TGACCAACAA TGTGCCCATT 
GCAAGGATTC TTCTGAGGAC AGGGGCCCGA GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC 
CGAGCAATGC ACCTCAACAC ACTGGTCCAG GAAGCCCAGG AGAGGGTGAG TGAACTGTCT 
GCCCAGGTGG AGAATGAAGG ATTCACTCTG GACAACACAG AGAAAGAGAA GCAGCTGAAA 
GCTTGGGAGT GGAGGTATCG GCTCTACAGA CGCATGAAAA CAGGCTTTGA GCATGCCAGA 
GCCCCTGAGA TGCCAACCAA TGTCTGTCTC ATGGTAACCA GCAGCACATC ACTCACTGTC 
AGCTTCCAAG AGCCTCTTAG CGTCAATGCA GCTGTAGTAA CCAGGTATAA AGTGGAATGG 
AGTATGTCCG AAGACTTTTC TCCTTTGGCT GGAGAAATCA TCATGGATAA TCTGCAGACT 
CTGAGATGCA CAATCACAGG ACTTACAATG GGCCAACAGT ATTTTGTTCA AGTCTCGGCT 
TACAATATGA AAGGATGGGG ACCTGCTCAG ACCACGACAC CGGCATGTGC CTCTCCTTCT 
AACTGGAAAG ACTATGACGA CAGAGAGCCC AGACACAAGG GACAGAGTGA AGTTTTGGAA 
GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT CAGCATTACA GTTGCCGGGA AAGCACAAAA 
TTACAAACCA CAGGCCGCAA GCAGTCAGTC TCAAGAAGCC TGAAACACCT GTTCCATTCC 
TCGAACAAGT TTGTGAAGAC CTTAAAACGG GGACTCTACA TAGCCGTTAT ATTTTATTAC 
AAAGACAATA TCTTAGTCAC CAATGAAGAT CAAGTACCAA TTGTTGAAAT AGATGACTCT 
CACACCAGTT CTATTACACA AGATTTTCTG TGGTTCACGA AGCTGTCTTG TATGTGGGAA 
GATATAAGGT GGCTGAGGCA AAGCATACCA ATATCCTCAT CCTCATCCAC AGTGCTGCAA 
ACTCGGCAGA AGATGCTCGC AGCAACAGCA CAGCTACAGA ATTTACTTGG GACACACAAC 
TTGGGAAGAG TTTACTATGA GCCCATTAAA GATCGACATG GAAACATACT CATAGTCACC 
ATCAGGGAGG TGGAGATGCT TTATTCATTT TTTAATGGCA AATGGATGCA GATCTCAAAG 
CTGCAAAGCC AGAGAAAGTC TCTATCAACA CCTGAGGAGC CAACAGCTTT AGACATTCTA 
CTGATAACCA TCCAGGATAT TCTATCCTAT CACAAAAGGA GTCATCAGCG TCTCTTTCCT 
GGATTATATC TGGGTTACCT AAAGCTCTGT AGCTCTGTGG ATCAAATCAA AGTTCTTGTT 
ACCCAAAAGT TGCCCAACAT TCTCTGCCAC GTGAAGATCC GTGAAAACAA TAATATTTCT 
AGAGAGGAAT GGGAATGGAT CCAAAAGCTT TCTGGCTCTG AATCTATGGA AAGTGTGGAT 
CATACTTCTG ACTGCCCCAT GCAATTGTTC TTCTACGAGC TCCAGATGGC AGTGAAAGCT 
CTCCTTCAGC AGATCAATAT ACCTCTACAC CAGGCAAGGA ACTTCCGCCT CTACACACAG 
GAGGTGTTGG AAATGGGTCA CAATGTGTCC TTTCTTCTCC TGCTCCCTGC CTCAGACGAC 
GTCTGTACAG CCCCAGGACA GAATAATCCT TACACCCCAC ACTCAGGGTT TCTTAACCTC 
CCTCTTCAGA TGTTTGAACT TGTTCATTTT TGCAGCTACA GAGAGAAATT TATTAGTCTG 
TATTGCCGCC TTTCTGCTGT TGTGGAGCTG GATTCTCTGA ACACCCAACA GTCCCTCAGG 
GAAGCAATCT CAGACAGCGA GGTTGCAGCT GCCAAACAAA GACACCAGCA AGTTTTAGAT 
TTCATTCAGC AAATAGATGA AGTCTGGCGT GAAATGAGAT GGATCATGGA TGCTCTACAG 
TATGCAAGAT ACAAACAACC AGTTTCTGGC TTGCCCATCA CTAAGCTGAT AGACCCCTCA 
GATGAGCAGA GCCTAAAGAA GATCAATTCT ACATCATCAT CACATATAGA CTGTCTTCCA 
TCCCCACCCC CATCCCCAGA GATGCACAGA AGAAAGACAG TGAGTGATTC ACAGCCCTGC 
TCTGATGAAG AAGCCTGCTC AGAAGTCTTC CTCCCCACCA ACAGTGACTA CGACTCCAGC 
GATGCCCTGA GCCCCAGAGA CCTGGACCTG GTCTACCTAT CATCTCACGA CATTGCGCAG 
CAGACCCTTA GCGGCCTAAG CGGCAGCGCC CCCGACGTCC TGCAAGTGCA CGACGTGAAA 
ACCCCTCTGG GGCCGGGCCA GGATCCCCAG GGCGAGGGCC CAAATCCCGA TCACTCATGT 
GCCGAGTTTC TCCATAGCCT GACCCTCACG GGGTTCACAC CCAAGAACCA CGCCAAGACT 
GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC CTGGGAAAGC GGAAGCCAGG CAAGCACCCC 
CACTATGGCG GCTTCAGCCG CCATCATCGC TGGTTGCGCA TCCACAGCGA GACCCAGTCG 
CTATCGCTCT CTGAGGGCAT TTATACACAG CACCTGTCCC AGGCCTGTGG TCTGGCCCAG 
GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT GCCCTTGATG ATCCCAGGGG CCTAACTCTG 
GCCCACGCTG CCAGCCTTCC TGAGGAGCGG AACAGCAGTC TCCAGGACGC GAGGCCTTCC 
GTCCGCCGCC TCTACGTGGA GCCCTACGCA GCGGCCGTGG TGGCCCAGGA CGAAAAACCA 
TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC CGCATCACCC TGCCCAGCCC CACTGGCCCC 
GATGTGAGTC AGGAGGGCCC CACCGCCTCT CCCATGTCAG AAATACTCAG CAGCATGCTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024267.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879756.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X7, mRNA.

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CloneID	OHu86638
Clone ID Related Accession (Same CDS sequence)	XM_017024267.1
Accession Version	XM_017024267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGGC ACAAAATGTC CACTCCACAA AAGAGAATAG GAAGGAGATT CGCTTGCTTT 
GCACAGAGGC TGAGCCACAG GAGAAAGCAA AGCCAATGTG ATTTATTGAA TGAAAGCACT 
GGACAATTAC CAACAACTTG TTCCTCTGCT GCCTCGAACA GCATAAACTG GAATTGTCGT 
GTGAAAATGA CGCAACAAAT GCAAAATTTA CATCTCTGTC AGTCAAAAAA ACATAGTGCT 
CCCTCATCTC CCAACGCAGC CAAACGCCTG TACAGGAACC TCTCTGAGAA ACTGAAAGGG 
AGCCACTCTT CCTTCGATGA GGCCTATTTT AGGACAAGAA CTGATCGGCT GAGTCTCAGG 
AAGACCTCGG TGAATTTCCA GGGCAATGAA GCCATGTTTG AGGCAGTCGA ACAGCAGGAC 
ATGGATGCTG TGCAGATCCT CCTGTATCAG TACACACCAG AAGAACTTGA CCTCAACACA 
CCTAACAGCG AGGGCTTGAC ACCCCTGGAT ATTGCCATCA TGACCAACAA TGTGCCCATT 
GCAAGGATTC TTCTGAGGAC AGGGGCCCGA GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC 
CGAGCAATGC ACCTCAACAC ACTGGTCCAG GAAGCCCAGG AGAGGGTGAG TGAACTGTCT 
GCCCAGGTGG AGAATGAAGG ATTCACTCTG GACAACACAG AGAAAGAGAA GCAGCTGAAA 
GCTTGGGAGT GGAGGTATCG GCTCTACAGA CGCATGAAAA CAGGCTTTGA GCATGCCAGA 
GCCCCTGAGA TGCCAACCAA TGTCTGTCTC ATGGTAACCA GCAGCACATC ACTCACTGTC 
AGCTTCCAAG AGCCTCTTAG CGTCAATGCA GCTGTAGTAA CCAGGTATAA AGTGGAATGG 
AGTATGTCCG AAGACTTTTC TCCTTTGGCT GGAGAAATCA TCATGGATAA TCTGCAGACT 
CTGAGATGCA CAATCACAGG ACTTACAATG GGCCAACAGT ATTTTGTTCA AGTCTCGGCT 
TACAATATGA AAGGATGGGG ACCTGCTCAG ACCACGACAC CGGCATGTGC CTCTCCTTCT 
AACTGGAAAG ACTATGACGA CAGAGAGCCC AGACACAAGG GACAGAGTGA AGTTTTGGAA 
GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT CAGCATTACA GTTGCCGGGA AAGCACAAAA 
TTACAAACCA CAGGCCGCAA GCAGTCAGTC TCAAGAAGCC TGAAACACCT GTTCCATTCC 
TCGAACAAGT TTGTGAAGAC CTTAAAACGG GGACTCTACA TAGCCGTTAT ATTTTATTAC 
AAAGACAATA TCTTAGTCAC CAATGAAGAT CAAGTACCAA TTGTTGAAAT AGATGACTCT 
CACACCAGTT CTATTACACA AGATTTTCTG TGGTTCACGA AGCTGTCTTG TATGTGGGAA 
GATATAAGGT GGCTGAGGCA AAGCATACCA ATATCCTCAT CCTCATCCAC AGTGCTGCAA 
ACTCGGCAGA AGATGCTCGC AGCAACAGCA CAGCTACAGA ATTTACTTGG GACACACAAC 
TTGGGAAGAG TTTACTATGA GCCCATTAAA GATCGACATG GAAACATACT CATAGTCACC 
ATCAGGGAGG TGGAGATGCT TTATTCATTT TTTAATGGCA AATGGATGCA GATCTCAAAG 
CTGCAAAGCC AGAGAAAGTC TCTATCAACA CCTGAGGAGC CAACAGCTTT AGACATTCTA 
CTGATAACCA TCCAGGATAT TCTATCCTAT CACAAAAGGA GTCATCAGCG TCTCTTTCCT 
GGATTATATC TGGGTTACCT AAAGCTCTGT AGCTCTGTGG ATCAAATCAA AGTTCTTGTT 
ACCCAAAAGT TGCCCAACAT TCTCTGCCAC GTGAAGATCC GTGAAAACAA TAATATTTCT 
AGAGAGGAAT GGGAATGGAT CCAAAAGCTT TCTGGCTCTG AATCTATGGA AAGTGTGGAT 
CATACTTCTG ACTGCCCCAT GCAATTGTTC TTCTACGAGC TCCAGATGGC AGTGAAAGCT 
CTCCTTCAGC AGATCAATAT ACCTCTACAC CAGGCAAGGA ACTTCCGCCT CTACACACAG 
GAGGTGTTGG AAATGGGTCA CAATGTGTCC TTTCTTCTCC TGCTCCCTGC CTCAGACGAC 
GTCTGTACAG CCCCAGGACA GAATAATCCT TACACCCCAC ACTCAGGGTT TCTTAACCTC 
CCTCTTCAGA TGTTTGAACT TGTTCATTTT TGCAGCTACA GAGAGAAATT TATTAGTCTG 
TATTGCCGCC TTTCTGCTGT TGTGGAGCTG GATTCTCTGA ACACCCAACA GTCCCTCAGG 
GAAGCAATCT CAGACAGCGA GGTTGCAGCT GCCAAACAAA GACACCAGCA AGTTTTAGAT 
TTCATTCAGC AAATAGATGA AGTCTGGCGT GAAATGAGAT GGATCATGGA TGCTCTACAG 
TATGCAAGAT ACAAACAACC AGTTTCTGGC TTGCCCATCA CTAAGCTGAT AGACCCCTCA 
GATGAGCAGA GCCTAAAGAA GATCAATTCT ACATCATCAT CACATATAGA CTGTCTTCCA 
TCCCCACCCC CATCCCCAGA GATGCACAGA AGAAAGACAG TGAGTGATTC ACAGCCCTGC 
TCTGATGAAG AAGCCTGCTC AGAAGTCTTC CTCCCCACCA ACAGTGACTA CGACTCCAGC 
GATGCCCTGA GCCCCAGAGA CCTGGACCTG GTCTACCTAT CATCTCACGA CATTGCGCAG 
CAGACCCTTA GCGGCCTAAG CGGCAGCGCC CCCGACGTCC TGCAAGTGCA CGACGTGAAA 
ACCCCTCTGG GGCCGGGCCA GGATCCCCAG GGCGAGGGCC CAAATCCCGA TCACTCATGT 
GCCGAGTTTC TCCATAGCCT GACCCTCACG GGGTTCACAC CCAAGAACCA CGCCAAGACT 
GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC CTGGGAAAGC GGAAGCCAGG CAAGCACCCC 
CACTATGGCG GCTTCAGCCG CCATCATCGC TGGTTGCGCA TCCACAGCGA GACCCAGTCG 
CTATCGCTCT CTGAGGGCAT TTATACACAG CACCTGTCCC AGGCCTGTGG TCTGGCCCAG 
GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT GCCCTTGATG ATCCCAGGGG CCTAACTCTG 
GCCCACGCTG CCAGCCTTCC TGAGGAGCGG AACAGCAGTC TCCAGGACGC GAGGCCTTCC 
GTCCGCCGCC TCTACGTGGA GCCCTACGCA GCGGCCGTGG TGGCCCAGGA CGAAAAACCA 
TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC CGCATCACCC TGCCCAGCCC CACTGGCCCC 
GATGTGAGTC AGGAGGGCCC CACCGCCTCT CCCATGTCAG AAATACTCAG CAGCATGCTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879756.1
CDS	87..3509
Translation	MQWHKMSTPQKRIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024267.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024267.1

ORF Insert Sequence:

ATGCAATGGC ACAAAATGTC CACTCCACAA AAGAGAATAG GAAGGAGATT CGCTTGCTTT 
GCACAGAGGC TGAGCCACAG GAGAAAGCAA AGCCAATGTG ATTTATTGAA TGAAAGCACT 
GGACAATTAC CAACAACTTG TTCCTCTGCT GCCTCGAACA GCATAAACTG GAATTGTCGT 
GTGAAAATGA CGCAACAAAT GCAAAATTTA CATCTCTGTC AGTCAAAAAA ACATAGTGCT 
CCCTCATCTC CCAACGCAGC CAAACGCCTG TACAGGAACC TCTCTGAGAA ACTGAAAGGG 
AGCCACTCTT CCTTCGATGA GGCCTATTTT AGGACAAGAA CTGATCGGCT GAGTCTCAGG 
AAGACCTCGG TGAATTTCCA GGGCAATGAA GCCATGTTTG AGGCAGTCGA ACAGCAGGAC 
ATGGATGCTG TGCAGATCCT CCTGTATCAG TACACACCAG AAGAACTTGA CCTCAACACA 
CCTAACAGCG AGGGCTTGAC ACCCCTGGAT ATTGCCATCA TGACCAACAA TGTGCCCATT 
GCAAGGATTC TTCTGAGGAC AGGGGCCCGA GAAAGTCCAC ACTTTGTCAG CCTGGAAAGC 
CGAGCAATGC ACCTCAACAC ACTGGTCCAG GAAGCCCAGG AGAGGGTGAG TGAACTGTCT 
GCCCAGGTGG AGAATGAAGG ATTCACTCTG GACAACACAG AGAAAGAGAA GCAGCTGAAA 
GCTTGGGAGT GGAGGTATCG GCTCTACAGA CGCATGAAAA CAGGCTTTGA GCATGCCAGA 
GCCCCTGAGA TGCCAACCAA TGTCTGTCTC ATGGTAACCA GCAGCACATC ACTCACTGTC 
AGCTTCCAAG AGCCTCTTAG CGTCAATGCA GCTGTAGTAA CCAGGTATAA AGTGGAATGG 
AGTATGTCCG AAGACTTTTC TCCTTTGGCT GGAGAAATCA TCATGGATAA TCTGCAGACT 
CTGAGATGCA CAATCACAGG ACTTACAATG GGCCAACAGT ATTTTGTTCA AGTCTCGGCT 
TACAATATGA AAGGATGGGG ACCTGCTCAG ACCACGACAC CGGCATGTGC CTCTCCTTCT 
AACTGGAAAG ACTATGACGA CAGAGAGCCC AGACACAAGG GACAGAGTGA AGTTTTGGAA 
GGTCTGCTGC AGCAGGTCCG AGCCCTTCAT CAGCATTACA GTTGCCGGGA AAGCACAAAA 
TTACAAACCA CAGGCCGCAA GCAGTCAGTC TCAAGAAGCC TGAAACACCT GTTCCATTCC 
TCGAACAAGT TTGTGAAGAC CTTAAAACGG GGACTCTACA TAGCCGTTAT ATTTTATTAC 
AAAGACAATA TCTTAGTCAC CAATGAAGAT CAAGTACCAA TTGTTGAAAT AGATGACTCT 
CACACCAGTT CTATTACACA AGATTTTCTG TGGTTCACGA AGCTGTCTTG TATGTGGGAA 
GATATAAGGT GGCTGAGGCA AAGCATACCA ATATCCTCAT CCTCATCCAC AGTGCTGCAA 
ACTCGGCAGA AGATGCTCGC AGCAACAGCA CAGCTACAGA ATTTACTTGG GACACACAAC 
TTGGGAAGAG TTTACTATGA GCCCATTAAA GATCGACATG GAAACATACT CATAGTCACC 
ATCAGGGAGG TGGAGATGCT TTATTCATTT TTTAATGGCA AATGGATGCA GATCTCAAAG 
CTGCAAAGCC AGAGAAAGTC TCTATCAACA CCTGAGGAGC CAACAGCTTT AGACATTCTA 
CTGATAACCA TCCAGGATAT TCTATCCTAT CACAAAAGGA GTCATCAGCG TCTCTTTCCT 
GGATTATATC TGGGTTACCT AAAGCTCTGT AGCTCTGTGG ATCAAATCAA AGTTCTTGTT 
ACCCAAAAGT TGCCCAACAT TCTCTGCCAC GTGAAGATCC GTGAAAACAA TAATATTTCT 
AGAGAGGAAT GGGAATGGAT CCAAAAGCTT TCTGGCTCTG AATCTATGGA AAGTGTGGAT 
CATACTTCTG ACTGCCCCAT GCAATTGTTC TTCTACGAGC TCCAGATGGC AGTGAAAGCT 
CTCCTTCAGC AGATCAATAT ACCTCTACAC CAGGCAAGGA ACTTCCGCCT CTACACACAG 
GAGGTGTTGG AAATGGGTCA CAATGTGTCC TTTCTTCTCC TGCTCCCTGC CTCAGACGAC 
GTCTGTACAG CCCCAGGACA GAATAATCCT TACACCCCAC ACTCAGGGTT TCTTAACCTC 
CCTCTTCAGA TGTTTGAACT TGTTCATTTT TGCAGCTACA GAGAGAAATT TATTAGTCTG 
TATTGCCGCC TTTCTGCTGT TGTGGAGCTG GATTCTCTGA ACACCCAACA GTCCCTCAGG 
GAAGCAATCT CAGACAGCGA GGTTGCAGCT GCCAAACAAA GACACCAGCA AGTTTTAGAT 
TTCATTCAGC AAATAGATGA AGTCTGGCGT GAAATGAGAT GGATCATGGA TGCTCTACAG 
TATGCAAGAT ACAAACAACC AGTTTCTGGC TTGCCCATCA CTAAGCTGAT AGACCCCTCA 
GATGAGCAGA GCCTAAAGAA GATCAATTCT ACATCATCAT CACATATAGA CTGTCTTCCA 
TCCCCACCCC CATCCCCAGA GATGCACAGA AGAAAGACAG TGAGTGATTC ACAGCCCTGC 
TCTGATGAAG AAGCCTGCTC AGAAGTCTTC CTCCCCACCA ACAGTGACTA CGACTCCAGC 
GATGCCCTGA GCCCCAGAGA CCTGGACCTG GTCTACCTAT CATCTCACGA CATTGCGCAG 
CAGACCCTTA GCGGCCTAAG CGGCAGCGCC CCCGACGTCC TGCAAGTGCA CGACGTGAAA 
ACCCCTCTGG GGCCGGGCCA GGATCCCCAG GGCGAGGGCC CAAATCCCGA TCACTCATGT 
GCCGAGTTTC TCCATAGCCT GACCCTCACG GGGTTCACAC CCAAGAACCA CGCCAAGACT 
GTGTCCGGTG GGCGGCCCCC GCTAGGCTTC CTGGGAAAGC GGAAGCCAGG CAAGCACCCC 
CACTATGGCG GCTTCAGCCG CCATCATCGC TGGTTGCGCA TCCACAGCGA GACCCAGTCG 
CTATCGCTCT CTGAGGGCAT TTATACACAG CACCTGTCCC AGGCCTGTGG TCTGGCCCAG 
GAGCCCAAGG AGGCCAAGCG GGCCGGCCCT GCCCTTGATG ATCCCAGGGG CCTAACTCTG 
GCCCACGCTG CCAGCCTTCC TGAGGAGCGG AACAGCAGTC TCCAGGACGC GAGGCCTTCC 
GTCCGCCGCC TCTACGTGGA GCCCTACGCA GCGGCCGTGG TGGCCCAGGA CGAAAAACCA 
TGGGCAAGCT TGAGCCCGCC CTCTGGAGGC CGCATCACCC TGCCCAGCCC CACTGGCCCC 
GATGTGAGTC AGGAGGGCCC CACCGCCTCT CCCATGTCAG AAATACTCAG CAGCATGCTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( NM_001365758.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352687.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 1, mRNA.

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CloneID	OHu50152
Clone ID Related Accession (Same CDS sequence)	XM_011524430.2 , XM_011524431.2 , XM_017024269.1 , XM_017024268.1 , NM_001365758.1 , XM_006721728.3
Accession Version	NM_001365758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC006925.6, AC006600.4 and AC015724.9. On Sep 12, 2018 this sequence version replaced XM_017024268.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.261127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END##

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352687.1
CDS	317..3553
Translation	MTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	NM_001365758.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365758.1

ORF Insert Sequence:

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_011524430.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522732.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu50152
Clone ID Related Accession (Same CDS sequence)	XM_011524430.2 , XM_011524431.2 , XM_017024269.1 , XM_017024268.1 , NM_001365758.1 , XM_006721728.3
Accession Version	XM_011524430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522732.1
CDS	372..3608
Translation	MTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_011524430.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524430.2

ORF Insert Sequence:

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_011524431.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522733.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu50152
Clone ID Related Accession (Same CDS sequence)	XM_011524430.2 , XM_011524431.2 , XM_017024269.1 , XM_017024268.1 , NM_001365758.1 , XM_006721728.3
Accession Version	XM_011524431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522733.1
CDS	304..3540
Translation	MTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_011524431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524431.2

ORF Insert Sequence:

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024269.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879758.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X12, mRNA.

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CloneID	OHu50152
Clone ID Related Accession (Same CDS sequence)	XM_011524430.2 , XM_011524431.2 , XM_017024269.1 , XM_017024268.1 , NM_001365758.1 , XM_006721728.3
Accession Version	XM_017024269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879758.1
CDS	546..3782
Translation	MTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024269.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024269.1

ORF Insert Sequence:

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_017024268.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879757.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu50152
Clone ID Related Accession (Same CDS sequence)	XM_011524430.2 , XM_011524431.2 , XM_017024269.1 , XM_017024268.1 , NM_001365758.1 , XM_006721728.3
Accession Version	XM_017024268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879757.1
CDS	1357..4593
Translation	MTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_017024268.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024268.1

ORF Insert Sequence:

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_006721728.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721791.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu50152
Clone ID Related Accession (Same CDS sequence)	XM_011524430.2 , XM_011524431.2 , XM_017024269.1 , XM_017024268.1 , NM_001365758.1 , XM_006721728.3
Accession Version	XM_006721728.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721791.1
CDS	472..3708
Translation	MTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_006721728.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721728.3

ORF Insert Sequence:

ATGACGCAAC AAATGCAAAA TTTACATCTC TGTCAGTCAA AAAAACATAG TGCTCCCTCA 
TCTCCCAACG CAGCCAAACG CCTGTACAGG AACCTCTCTG AGAAACTGAA AGGGAGCCAC 
TCTTCCTTCG ATGAGGCCTA TTTTAGGACA AGAACTGATC GGCTGAGTCT CAGGAAGACC 
TCGGTGAATT TCCAGGGCAA TGAAGCCATG TTTGAGGCAG TCGAACAGCA GGACATGGAT 
GCTGTGCAGA TCCTCCTGTA TCAGTACACA CCAGAAGAAC TTGACCTCAA CACACCTAAC 
AGCGAGGGCT TGACACCCCT GGATATTGCC ATCATGACCA ACAATGTGCC CATTGCAAGG 
ATTCTTCTGA GGACAGGGGC CCGAGAAAGT CCACACTTTG TCAGCCTGGA AAGCCGAGCA 
ATGCACCTCA ACACACTGGT CCAGGAAGCC CAGGAGAGGG TGAGTGAACT GTCTGCCCAG 
GTGGAGAATG AAGGATTCAC TCTGGACAAC ACAGAGAAAG AGAAGCAGCT GAAAGCTTGG 
GAGTGGAGGT ATCGGCTCTA CAGACGCATG AAAACAGGCT TTGAGCATGC CAGAGCCCCT 
GAGATGCCAA CCAATGTCTG TCTCATGGTA ACCAGCAGCA CATCACTCAC TGTCAGCTTC 
CAAGAGCCTC TTAGCGTCAA TGCAGCTGTA GTAACCAGGT ATAAAGTGGA ATGGAGTATG 
TCCGAAGACT TTTCTCCTTT GGCTGGAGAA ATCATCATGG ATAATCTGCA GACTCTGAGA 
TGCACAATCA CAGGACTTAC AATGGGCCAA CAGTATTTTG TTCAAGTCTC GGCTTACAAT 
ATGAAAGGAT GGGGACCTGC TCAGACCACG ACACCGGCAT GTGCCTCTCC TTCTAACTGG 
AAAGACTATG ACGACAGAGA GCCCAGACAC AAGGGACAGA GTGAAGTTTT GGAAGGTCTG 
CTGCAGCAGG TCCGAGCCCT TCATCAGCAT TACAGTTGCC GGGAAAGCAC AAAATTACAA 
ACCACAGGCC GCAAGCAGTC AGTCTCAAGA AGCCTGAAAC ACCTGTTCCA TTCCTCGAAC 
AAGTTTGTGA AGACCTTAAA ACGGGGACTC TACATAGCCG TTATATTTTA TTACAAAGAC 
AATATCTTAG TCACCAATGA AGATCAAGTA CCAATTGTTG AAATAGATGA CTCTCACACC 
AGTTCTATTA CACAAGATTT TCTGTGGTTC ACGAAGCTGT CTTGTATGTG GGAAGATATA 
AGGTGGCTGA GGCAAAGCAT ACCAATATCC TCATCCTCAT CCACAGTGCT GCAAACTCGG 
CAGAAGATGC TCGCAGCAAC AGCACAGCTA CAGAATTTAC TTGGGACACA CAACTTGGGA 
AGAGTTTACT ATGAGCCCAT TAAAGATCGA CATGGAAACA TACTCATAGT CACCATCAGG 
GAGGTGGAGA TGCTTTATTC ATTTTTTAAT GGCAAATGGA TGCAGATCTC AAAGCTGCAA 
AGCCAGAGAA AGTCTCTATC AACACCTGAG GAGCCAACAG CTTTAGACAT TCTACTGATA 
ACCATCCAGG ATATTCTATC CTATCACAAA AGGAGTCATC AGCGTCTCTT TCCTGGATTA 
TATCTGGGTT ACCTAAAGCT CTGTAGCTCT GTGGATCAAA TCAAAGTTCT TGTTACCCAA 
AAGTTGCCCA ACATTCTCTG CCACGTGAAG ATCCGTGAAA ACAATAATAT TTCTAGAGAG 
GAATGGGAAT GGATCCAAAA GCTTTCTGGC TCTGAATCTA TGGAAAGTGT GGATCATACT 
TCTGACTGCC CCATGCAATT GTTCTTCTAC GAGCTCCAGA TGGCAGTGAA AGCTCTCCTT 
CAGCAGATCA ATATACCTCT ACACCAGGCA AGGAACTTCC GCCTCTACAC ACAGGAGGTG 
TTGGAAATGG GTCACAATGT GTCCTTTCTT CTCCTGCTCC CTGCCTCAGA CGACGTCTGT 
ACAGCCCCAG GACAGAATAA TCCTTACACC CCACACTCAG GGTTTCTTAA CCTCCCTCTT 
CAGATGTTTG AACTTGTTCA TTTTTGCAGC TACAGAGAGA AATTTATTAG TCTGTATTGC 
CGCCTTTCTG CTGTTGTGGA GCTGGATTCT CTGAACACCC AACAGTCCCT CAGGGAAGCA 
ATCTCAGACA GCGAGGTTGC AGCTGCCAAA CAAAGACACC AGCAAGTTTT AGATTTCATT 
CAGCAAATAG ATGAAGTCTG GCGTGAAATG AGATGGATCA TGGATGCTCT ACAGTATGCA 
AGATACAAAC AACCAGTTTC TGGCTTGCCC ATCACTAAGC TGATAGACCC CTCAGATGAG 
CAGAGCCTAA AGAAGATCAA TTCTACATCA TCATCACATA TAGACTGTCT TCCATCCCCA 
CCCCCATCCC CAGAGATGCA CAGAAGAAAG ACAGTGAGTG ATTCACAGCC CTGCTCTGAT 
GAAGAAGCCT GCTCAGAAGT CTTCCTCCCC ACCAACAGTG ACTACGACTC CAGCGATGCC 
CTGAGCCCCA GAGACCTGGA CCTGGTCTAC CTATCATCTC ACGACATTGC GCAGCAGACC 
CTTAGCGGCC TAAGCGGCAG CGCCCCCGAC GTCCTGCAAG TGCACGACGT GAAAACCCCT 
CTGGGGCCGG GCCAGGATCC CCAGGGCGAG GGCCCAAATC CCGATCACTC ATGTGCCGAG 
TTTCTCCATA GCCTGACCCT CACGGGGTTC ACACCCAAGA ACCACGCCAA GACTGTGTCC 
GGTGGGCGGC CCCCGCTAGG CTTCCTGGGA AAGCGGAAGC CAGGCAAGCA CCCCCACTAT 
GGCGGCTTCA GCCGCCATCA TCGCTGGTTG CGCATCCACA GCGAGACCCA GTCGCTATCG 
CTCTCTGAGG GCATTTATAC ACAGCACCTG TCCCAGGCCT GTGGTCTGGC CCAGGAGCCC 
AAGGAGGCCA AGCGGGCCGG CCCTGCCCTT GATGATCCCA GGGGCCTAAC TCTGGCCCAC 
GCTGCCAGCC TTCCTGAGGA GCGGAACAGC AGTCTCCAGG ACGCGAGGCC TTCCGTCCGC 
CGCCTCTACG TGGAGCCCTA CGCAGCGGCC GTGGTGGCCC AGGACGAAAA ACCATGGGCA 
AGCTTGAGCC CGCCCTCTGG AGGCCGCATC ACCCTGCCCA GCCCCACTGG CCCCGATGTG 
AGTCAGGAGG GCCCCACCGC CTCTCCCATG TCAGAAATAC TCAGCAGCAT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_011524428.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522730.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu77166
Clone ID Related Accession (Same CDS sequence)	XM_011524428.2
Accession Version	XM_011524428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGT CTCTAACCAG GAGGCTACTC TTTAAAGACA GGCATTTTAC TTGCAGCAAA 
ATAATAGGAA GGAGATTCGC TTGCTTTGCA CAGAGGCTGA GCCACAGGAG AAAGCAAAGC 
CAATGTGATT TATTGAATGA AAGCACTGGA CAATTACCAA CAACTTGTTC CTCTGCTGCC 
TCGAACAGCA TAAACTGGAA TTGTCGTGTG AAAATGACGC AACAAATGCA AAATTTACAT 
CTCTGTCAGT CAAAAAAACA TAGTGCTCCC TCATCTCCCA ACGCAGCCAA ACGCCTGTAC 
AGGAACCTCT CTGAGAAACT GAAAGGGAGC CACTCTTCCT TCGATGAGGC CTATTTTAGG 
ACAAGAACTG ATCGGCTGAG TCTCAGGAAG ACCTCGGTGA ATTTCCAGGG CAATGAAGCC 
ATGTTTGAGG CAGTCGAACA GCAGGACATG GATGCTGTGC AGATCCTCCT GTATCAGTAC 
ACACCAGAAG AACTTGACCT CAACACACCT AACAGCGAGG GCTTGACACC CCTGGATATT 
GCCATCATGA CCAACAATGT GCCCATTGCA AGGATTCTTC TGAGGACAGG GGCCCGAGAA 
AGTCCACACT TTGTCAGCCT GGAAAGCCGA GCAATGCACC TCAACACACT GGTCCAGGAA 
GCCCAGGAGA GGGTGAGTGA ACTGTCTGCC CAGGTGGAGA ATGAAGGATT CACTCTGGAC 
AACACAGAGA AAGAGAAGCA GCTGAAAGCT TGGGAGTGGA GGTATCGGCT CTACAGACGC 
ATGAAAACAG GCTTTGAGCA TGCCAGAGCC CCTGAGATGC CAACCAATGT CTGTCTCATG 
GTAACCAGCA GCACATCACT CACTGTCAGC TTCCAAGAGC CTCTTAGCGT CAATGCAGCT 
GTAGTAACCA GGTATAAAGT GGAATGGAGT ATGTCCGAAG ACTTTTCTCC TTTGGCTGGA 
GAAATCATCA TGGATAATCT GCAGACTCTG AGATGCACAA TCACAGGACT TACAATGGGC 
CAACAGTATT TTGTTCAAGT CTCGGCTTAC AATATGAAAG GATGGGGACC TGCTCAGACC 
ACGACACCGG CATGTGCCTC TCCTTCTAAC TGGAAAGACT ATGACGACAG AGAGCCCAGA 
CACAAGGGAC AGAGTGAAGT TTTGGAAGGT CTGCTGCAGC AGGTCCGAGC CCTTCATCAG 
CATTACAGTT GCCGGGAAAG CACAAAATTA CAAACCACAG GCCGCAAGCA GTCAGTCTCA 
AGAAGCCTGA AACACCTGTT CCATTCCTCG AACAAGTTTG TGAAGACCTT AAAACGGGGA 
CTCTACATAG CCGTTATATT TTATTACAAA GACAATATCT TAGTCACCAA TGAAGATCAA 
GTACCAATTG TTGAAATAGA TGACTCTCAC ACCAGTTCTA TTACACAAGA TTTTCTGTGG 
TTCACGAAGC TGTCTTGTAT GTGGGAAGAT ATAAGGTGGC TGAGGCAAAG CATACCAATA 
TCCTCATCCT CATCCACAGT GCTGCAAACT CGGCAGAAGA TGCTCGCAGC AACAGCACAG 
CTACAGAATT TACTTGGGAC ACACAACTTG GGAAGAGTTT ACTATGAGCC CATTAAAGAT 
CGACATGGAA ACATACTCAT AGTCACCATC AGGGAGGTGG AGATGCTTTA TTCATTTTTT 
AATGGCAAAT GGATGCAGAT CTCAAAGCTG CAAAGCCAGA GAAAGTCTCT ATCAACACCT 
GAGGAGCCAA CAGCTTTAGA CATTCTACTG ATAACCATCC AGGATATTCT ATCCTATCAC 
AAAAGGAGTC ATCAGCGTCT CTTTCCTGGA TTATATCTGG GTTACCTAAA GCTCTGTAGC 
TCTGTGGATC AAATCAAAGT TCTTGTTACC CAAAAGTTGC CCAACATTCT CTGCCACGTG 
AAGATCCGTG AAAACAATAA TATTTCTAGA GAGGAATGGG AATGGATCCA AAAGCTTTCT 
GGCTCTGAAT CTATGGAAAG TGTGGATCAT ACTTCTGACT GCCCCATGCA ATTGTTCTTC 
TACGAGCTCC AGATGGCAGT GAAAGCTCTC CTTCAGCAGA TCAATATACC TCTACACCAG 
GCAAGGAACT TCCGCCTCTA CACACAGGAG GTGTTGGAAA TGGGTCACAA TGTGTCCTTT 
CTTCTCCTGC TCCCTGCCTC AGACGACGTC TGTACAGCCC CAGGACAGAA TAATCCTTAC 
ACCCCACACT CAGGGTTTCT TAACCTCCCT CTTCAGATGT TTGAACTTGT TCATTTTTGC 
AGCTACAGAG AGAAATTTAT TAGTCTGTAT TGCCGCCTTT CTGCTGTTGT GGAGCTGGAT 
TCTCTGAACA CCCAACAGTC CCTCAGGGAA GCAATCTCAG ACAGCGAGGT TGCAGCTGCC 
AAACAAAGAC ACCAGCAAGT TTTAGATTTC ATTCAGCAAA TAGATGAAGT CTGGCGTGAA 
ATGAGATGGA TCATGGATGC TCTACAGTAT GCAAGATACA AACAACCAGT TTCTGGCTTG 
CCCATCACTA AGCTGATAGA CCCCTCAGAT GAGCAGAGCC TAAAGAAGAT CAATTCTACA 
TCATCATCAC ATATAGACTG TCTTCCATCC CCACCCCCAT CCCCAGAGAT GCACAGAAGA 
AAGACAGTGA GTGATTCACA GCCCTGCTCT GATGAAGAAG CCTGCTCAGA AGTCTTCCTC 
CCCACCAACA GTGACTACGA CTCCAGCGAT GCCCTGAGCC CCAGAGACCT GGACCTGGTC 
TACCTATCAT CTCACGACAT TGCGCAGCAG ACCCTTAGCG GCCTAAGCGG CAGCGCCCCC 
GACGTCCTGC AAGTGCACGA CGTGAAAACC CCTCTGGGGC CGGGCCAGGA TCCCCAGGGC 
GAGGGCCCAA ATCCCGATCA CTCATGTGCC GAGTTTCTCC ATAGCCTGAC CCTCACGGGG 
TTCACACCCA AGAACCACGC CAAGACTGTG TCCGGTGGGC GGCCCCCGCT AGGCTTCCTG 
GGAAAGCGGA AGCCAGGCAA GCACCCCCAC TATGGCGGCT TCAGCCGCCA TCATCGCTGG 
TTGCGCATCC ACAGCGAGAC CCAGTCGCTA TCGCTCTCTG AGGGCATTTA TACACAGCAC 
CTGTCCCAGG CCTGTGGTCT GGCCCAGGAG CCCAAGGAGG CCAAGCGGGC CGGCCCTGCC 
CTTGATGATC CCAGGGGCCT AACTCTGGCC CACGCTGCCA GCCTTCCTGA GGAGCGGAAC 
AGCAGTCTCC AGGACGCGAG GCCTTCCGTC CGCCGCCTCT ACGTGGAGCC CTACGCAGCG 
GCCGTGGTGG CCCAGGACGA AAAACCATGG GCAAGCTTGA GCCCGCCCTC TGGAGGCCGC 
ATCACCCTGC CCAGCCCCAC TGGCCCCGAT GTGAGTCAGG AGGGCCCCAC CGCCTCTCCC 
ATGTCAGAAA TACTCAGCAG CATGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522730.1
CDS	36..3485
Translation	MEASLTRRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_011524428.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524428.2

ORF Insert Sequence:

ATGGAGGCGT CTCTAACCAG GAGGCTACTC TTTAAAGACA GGCATTTTAC TTGCAGCAAA 
ATAATAGGAA GGAGATTCGC TTGCTTTGCA CAGAGGCTGA GCCACAGGAG AAAGCAAAGC 
CAATGTGATT TATTGAATGA AAGCACTGGA CAATTACCAA CAACTTGTTC CTCTGCTGCC 
TCGAACAGCA TAAACTGGAA TTGTCGTGTG AAAATGACGC AACAAATGCA AAATTTACAT 
CTCTGTCAGT CAAAAAAACA TAGTGCTCCC TCATCTCCCA ACGCAGCCAA ACGCCTGTAC 
AGGAACCTCT CTGAGAAACT GAAAGGGAGC CACTCTTCCT TCGATGAGGC CTATTTTAGG 
ACAAGAACTG ATCGGCTGAG TCTCAGGAAG ACCTCGGTGA ATTTCCAGGG CAATGAAGCC 
ATGTTTGAGG CAGTCGAACA GCAGGACATG GATGCTGTGC AGATCCTCCT GTATCAGTAC 
ACACCAGAAG AACTTGACCT CAACACACCT AACAGCGAGG GCTTGACACC CCTGGATATT 
GCCATCATGA CCAACAATGT GCCCATTGCA AGGATTCTTC TGAGGACAGG GGCCCGAGAA 
AGTCCACACT TTGTCAGCCT GGAAAGCCGA GCAATGCACC TCAACACACT GGTCCAGGAA 
GCCCAGGAGA GGGTGAGTGA ACTGTCTGCC CAGGTGGAGA ATGAAGGATT CACTCTGGAC 
AACACAGAGA AAGAGAAGCA GCTGAAAGCT TGGGAGTGGA GGTATCGGCT CTACAGACGC 
ATGAAAACAG GCTTTGAGCA TGCCAGAGCC CCTGAGATGC CAACCAATGT CTGTCTCATG 
GTAACCAGCA GCACATCACT CACTGTCAGC TTCCAAGAGC CTCTTAGCGT CAATGCAGCT 
GTAGTAACCA GGTATAAAGT GGAATGGAGT ATGTCCGAAG ACTTTTCTCC TTTGGCTGGA 
GAAATCATCA TGGATAATCT GCAGACTCTG AGATGCACAA TCACAGGACT TACAATGGGC 
CAACAGTATT TTGTTCAAGT CTCGGCTTAC AATATGAAAG GATGGGGACC TGCTCAGACC 
ACGACACCGG CATGTGCCTC TCCTTCTAAC TGGAAAGACT ATGACGACAG AGAGCCCAGA 
CACAAGGGAC AGAGTGAAGT TTTGGAAGGT CTGCTGCAGC AGGTCCGAGC CCTTCATCAG 
CATTACAGTT GCCGGGAAAG CACAAAATTA CAAACCACAG GCCGCAAGCA GTCAGTCTCA 
AGAAGCCTGA AACACCTGTT CCATTCCTCG AACAAGTTTG TGAAGACCTT AAAACGGGGA 
CTCTACATAG CCGTTATATT TTATTACAAA GACAATATCT TAGTCACCAA TGAAGATCAA 
GTACCAATTG TTGAAATAGA TGACTCTCAC ACCAGTTCTA TTACACAAGA TTTTCTGTGG 
TTCACGAAGC TGTCTTGTAT GTGGGAAGAT ATAAGGTGGC TGAGGCAAAG CATACCAATA 
TCCTCATCCT CATCCACAGT GCTGCAAACT CGGCAGAAGA TGCTCGCAGC AACAGCACAG 
CTACAGAATT TACTTGGGAC ACACAACTTG GGAAGAGTTT ACTATGAGCC CATTAAAGAT 
CGACATGGAA ACATACTCAT AGTCACCATC AGGGAGGTGG AGATGCTTTA TTCATTTTTT 
AATGGCAAAT GGATGCAGAT CTCAAAGCTG CAAAGCCAGA GAAAGTCTCT ATCAACACCT 
GAGGAGCCAA CAGCTTTAGA CATTCTACTG ATAACCATCC AGGATATTCT ATCCTATCAC 
AAAAGGAGTC ATCAGCGTCT CTTTCCTGGA TTATATCTGG GTTACCTAAA GCTCTGTAGC 
TCTGTGGATC AAATCAAAGT TCTTGTTACC CAAAAGTTGC CCAACATTCT CTGCCACGTG 
AAGATCCGTG AAAACAATAA TATTTCTAGA GAGGAATGGG AATGGATCCA AAAGCTTTCT 
GGCTCTGAAT CTATGGAAAG TGTGGATCAT ACTTCTGACT GCCCCATGCA ATTGTTCTTC 
TACGAGCTCC AGATGGCAGT GAAAGCTCTC CTTCAGCAGA TCAATATACC TCTACACCAG 
GCAAGGAACT TCCGCCTCTA CACACAGGAG GTGTTGGAAA TGGGTCACAA TGTGTCCTTT 
CTTCTCCTGC TCCCTGCCTC AGACGACGTC TGTACAGCCC CAGGACAGAA TAATCCTTAC 
ACCCCACACT CAGGGTTTCT TAACCTCCCT CTTCAGATGT TTGAACTTGT TCATTTTTGC 
AGCTACAGAG AGAAATTTAT TAGTCTGTAT TGCCGCCTTT CTGCTGTTGT GGAGCTGGAT 
TCTCTGAACA CCCAACAGTC CCTCAGGGAA GCAATCTCAG ACAGCGAGGT TGCAGCTGCC 
AAACAAAGAC ACCAGCAAGT TTTAGATTTC ATTCAGCAAA TAGATGAAGT CTGGCGTGAA 
ATGAGATGGA TCATGGATGC TCTACAGTAT GCAAGATACA AACAACCAGT TTCTGGCTTG 
CCCATCACTA AGCTGATAGA CCCCTCAGAT GAGCAGAGCC TAAAGAAGAT CAATTCTACA 
TCATCATCAC ATATAGACTG TCTTCCATCC CCACCCCCAT CCCCAGAGAT GCACAGAAGA 
AAGACAGTGA GTGATTCACA GCCCTGCTCT GATGAAGAAG CCTGCTCAGA AGTCTTCCTC 
CCCACCAACA GTGACTACGA CTCCAGCGAT GCCCTGAGCC CCAGAGACCT GGACCTGGTC 
TACCTATCAT CTCACGACAT TGCGCAGCAG ACCCTTAGCG GCCTAAGCGG CAGCGCCCCC 
GACGTCCTGC AAGTGCACGA CGTGAAAACC CCTCTGGGGC CGGGCCAGGA TCCCCAGGGC 
GAGGGCCCAA ATCCCGATCA CTCATGTGCC GAGTTTCTCC ATAGCCTGAC CCTCACGGGG 
TTCACACCCA AGAACCACGC CAAGACTGTG TCCGGTGGGC GGCCCCCGCT AGGCTTCCTG 
GGAAAGCGGA AGCCAGGCAA GCACCCCCAC TATGGCGGCT TCAGCCGCCA TCATCGCTGG 
TTGCGCATCC ACAGCGAGAC CCAGTCGCTA TCGCTCTCTG AGGGCATTTA TACACAGCAC 
CTGTCCCAGG CCTGTGGTCT GGCCCAGGAG CCCAAGGAGG CCAAGCGGGC CGGCCCTGCC 
CTTGATGATC CCAGGGGCCT AACTCTGGCC CACGCTGCCA GCCTTCCTGA GGAGCGGAAC 
AGCAGTCTCC AGGACGCGAG GCCTTCCGTC CGCCGCCTCT ACGTGGAGCC CTACGCAGCG 
GCCGTGGTGG CCCAGGACGA AAAACCATGG GCAAGCTTGA GCCCGCCCTC TGGAGGCCGC 
ATCACCCTGC CCAGCCCCAC TGGCCCCGAT GTGAGTCAGG AGGGCCCCAC CGCCTCTCCC 
ATGTCAGAAA TACTCAGCAG CATGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKFN1 ( XM_011524429.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522731.1 Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu77167
Clone ID Related Accession (Same CDS sequence)	XM_011524429.2
Accession Version	XM_011524429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTAC TCTTTAAAGA CAGGCATTTT ACTTGCAGCA AAATAATAGG AAGGAGATTC 
GCTTGCTTTG CACAGAGGCT GAGCCACAGG AGAAAGCAAA GCCAATGTGA TTTATTGAAT 
GAAAGCACTG GACAATTACC AACAACTTGT TCCTCTGCTG CCTCGAACAG CATAAACTGG 
AATTGTCGTG TGAAAATGAC GCAACAAATG CAAAATTTAC ATCTCTGTCA GTCAAAAAAA 
CATAGTGCTC CCTCATCTCC CAACGCAGCC AAACGCCTGT ACAGGAACCT CTCTGAGAAA 
CTGAAAGGGA GCCACTCTTC CTTCGATGAG GCCTATTTTA GGACAAGAAC TGATCGGCTG 
AGTCTCAGGA AGACCTCGGT GAATTTCCAG GGCAATGAAG CCATGTTTGA GGCAGTCGAA 
CAGCAGGACA TGGATGCTGT GCAGATCCTC CTGTATCAGT ACACACCAGA AGAACTTGAC 
CTCAACACAC CTAACAGCGA GGGCTTGACA CCCCTGGATA TTGCCATCAT GACCAACAAT 
GTGCCCATTG CAAGGATTCT TCTGAGGACA GGGGCCCGAG AAAGTCCACA CTTTGTCAGC 
CTGGAAAGCC GAGCAATGCA CCTCAACACA CTGGTCCAGG AAGCCCAGGA GAGGGTGAGT 
GAACTGTCTG CCCAGGTGGA GAATGAAGGA TTCACTCTGG ACAACACAGA GAAAGAGAAG 
CAGCTGAAAG CTTGGGAGTG GAGGTATCGG CTCTACAGAC GCATGAAAAC AGGCTTTGAG 
CATGCCAGAG CCCCTGAGAT GCCAACCAAT GTCTGTCTCA TGGTAACCAG CAGCACATCA 
CTCACTGTCA GCTTCCAAGA GCCTCTTAGC GTCAATGCAG CTGTAGTAAC CAGGTATAAA 
GTGGAATGGA GTATGTCCGA AGACTTTTCT CCTTTGGCTG GAGAAATCAT CATGGATAAT 
CTGCAGACTC TGAGATGCAC AATCACAGGA CTTACAATGG GCCAACAGTA TTTTGTTCAA 
GTCTCGGCTT ACAATATGAA AGGATGGGGA CCTGCTCAGA CCACGACACC GGCATGTGCC 
TCTCCTTCTA ACTGGAAAGA CTATGACGAC AGAGAGCCCA GACACAAGGG ACAGAGTGAA 
GTTTTGGAAG GTCTGCTGCA GCAGGTCCGA GCCCTTCATC AGCATTACAG TTGCCGGGAA 
AGCACAAAAT TACAAACCAC AGGCCGCAAG CAGTCAGTCT CAAGAAGCCT GAAACACCTG 
TTCCATTCCT CGAACAAGTT TGTGAAGACC TTAAAACGGG GACTCTACAT AGCCGTTATA 
TTTTATTACA AAGACAATAT CTTAGTCACC AATGAAGATC AAGTACCAAT TGTTGAAATA 
GATGACTCTC ACACCAGTTC TATTACACAA GATTTTCTGT GGTTCACGAA GCTGTCTTGT 
ATGTGGGAAG ATATAAGGTG GCTGAGGCAA AGCATACCAA TATCCTCATC CTCATCCACA 
GTGCTGCAAA CTCGGCAGAA GATGCTCGCA GCAACAGCAC AGCTACAGAA TTTACTTGGG 
ACACACAACT TGGGAAGAGT TTACTATGAG CCCATTAAAG ATCGACATGG AAACATACTC 
ATAGTCACCA TCAGGGAGGT GGAGATGCTT TATTCATTTT TTAATGGCAA ATGGATGCAG 
ATCTCAAAGC TGCAAAGCCA GAGAAAGTCT CTATCAACAC CTGAGGAGCC AACAGCTTTA 
GACATTCTAC TGATAACCAT CCAGGATATT CTATCCTATC ACAAAAGGAG TCATCAGCGT 
CTCTTTCCTG GATTATATCT GGGTTACCTA AAGCTCTGTA GCTCTGTGGA TCAAATCAAA 
GTTCTTGTTA CCCAAAAGTT GCCCAACATT CTCTGCCACG TGAAGATCCG TGAAAACAAT 
AATATTTCTA GAGAGGAATG GGAATGGATC CAAAAGCTTT CTGGCTCTGA ATCTATGGAA 
AGTGTGGATC ATACTTCTGA CTGCCCCATG CAATTGTTCT TCTACGAGCT CCAGATGGCA 
GTGAAAGCTC TCCTTCAGCA GATCAATATA CCTCTACACC AGGCAAGGAA CTTCCGCCTC 
TACACACAGG AGGTGTTGGA AATGGGTCAC AATGTGTCCT TTCTTCTCCT GCTCCCTGCC 
TCAGACGACG TCTGTACAGC CCCAGGACAG AATAATCCTT ACACCCCACA CTCAGGGTTT 
CTTAACCTCC CTCTTCAGAT GTTTGAACTT GTTCATTTTT GCAGCTACAG AGAGAAATTT 
ATTAGTCTGT ATTGCCGCCT TTCTGCTGTT GTGGAGCTGG ATTCTCTGAA CACCCAACAG 
TCCCTCAGGG AAGCAATCTC AGACAGCGAG GTTGCAGCTG CCAAACAAAG ACACCAGCAA 
GTTTTAGATT TCATTCAGCA AATAGATGAA GTCTGGCGTG AAATGAGATG GATCATGGAT 
GCTCTACAGT ATGCAAGATA CAAACAACCA GTTTCTGGCT TGCCCATCAC TAAGCTGATA 
GACCCCTCAG ATGAGCAGAG CCTAAAGAAG ATCAATTCTA CATCATCATC ACATATAGAC 
TGTCTTCCAT CCCCACCCCC ATCCCCAGAG ATGCACAGAA GAAAGACAGT GAGTGATTCA 
CAGCCCTGCT CTGATGAAGA AGCCTGCTCA GAAGTCTTCC TCCCCACCAA CAGTGACTAC 
GACTCCAGCG ATGCCCTGAG CCCCAGAGAC CTGGACCTGG TCTACCTATC ATCTCACGAC 
ATTGCGCAGC AGACCCTTAG CGGCCTAAGC GGCAGCGCCC CCGACGTCCT GCAAGTGCAC 
GACGTGAAAA CCCCTCTGGG GCCGGGCCAG GATCCCCAGG GCGAGGGCCC AAATCCCGAT 
CACTCATGTG CCGAGTTTCT CCATAGCCTG ACCCTCACGG GGTTCACACC CAAGAACCAC 
GCCAAGACTG TGTCCGGTGG GCGGCCCCCG CTAGGCTTCC TGGGAAAGCG GAAGCCAGGC 
AAGCACCCCC ACTATGGCGG CTTCAGCCGC CATCATCGCT GGTTGCGCAT CCACAGCGAG 
ACCCAGTCGC TATCGCTCTC TGAGGGCATT TATACACAGC ACCTGTCCCA GGCCTGTGGT 
CTGGCCCAGG AGCCCAAGGA GGCCAAGCGG GCCGGCCCTG CCCTTGATGA TCCCAGGGGC 
CTAACTCTGG CCCACGCTGC CAGCCTTCCT GAGGAGCGGA ACAGCAGTCT CCAGGACGCG 
AGGCCTTCCG TCCGCCGCCT CTACGTGGAG CCCTACGCAG CGGCCGTGGT GGCCCAGGAC 
GAAAAACCAT GGGCAAGCTT GAGCCCGCCC TCTGGAGGCC GCATCACCCT GCCCAGCCCC 
ACTGGCCCCG ATGTGAGTCA GGAGGGCCCC ACCGCCTCTC CCATGTCAGA AATACTCAGC 
AGCATGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522731.1
CDS	211..3642
Translation	MRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRVKMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEAMFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESRAMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLMVTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAYNMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVSRSLKHLFHSSNKFVKTLKRGLYIAVIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWEDIRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVTQKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCSYREKFISLYCRLSAVVELDSLNTQQSLREAISDSEVAAAKQRHQQVLDFIQQIDEVWREMRWIMDALQYARYKQPVSGLPITKLIDPSDEQSLKKINSTSSSHIDCLPSPPPSPEMHRRKTVSDSQPCSDEEACSEVFLPTNSDYDSSDALSPRDLDLVYLSSHDIAQQTLSGLSGSAPDVLQVHDVKTPLGPGQDPQGEGPNPDHSCAEFLHSLTLTGFTPKNHAKTVSGGRPPLGFLGKRKPGKHPHYGGFSRHHRWLRIHSETQSLSLSEGIYTQHLSQACGLAQEPKEAKRAGPALDDPRGLTLAHAASLPEERNSSLQDARPSVRRLYVEPYAAAVVAQDEKPWASLSPPSGGRITLPSPTGPDVSQEGPTASPMSEILSSML

Target ORF information:

RefSeq Version	XM_011524429.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and fibronectin type III domain containing 1 (ANKFN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524429.2

ORF Insert Sequence:

ATGAGGCTAC TCTTTAAAGA CAGGCATTTT ACTTGCAGCA AAATAATAGG AAGGAGATTC 
GCTTGCTTTG CACAGAGGCT GAGCCACAGG AGAAAGCAAA GCCAATGTGA TTTATTGAAT 
GAAAGCACTG GACAATTACC AACAACTTGT TCCTCTGCTG CCTCGAACAG CATAAACTGG 
AATTGTCGTG TGAAAATGAC GCAACAAATG CAAAATTTAC ATCTCTGTCA GTCAAAAAAA 
CATAGTGCTC CCTCATCTCC CAACGCAGCC AAACGCCTGT ACAGGAACCT CTCTGAGAAA 
CTGAAAGGGA GCCACTCTTC CTTCGATGAG GCCTATTTTA GGACAAGAAC TGATCGGCTG 
AGTCTCAGGA AGACCTCGGT GAATTTCCAG GGCAATGAAG CCATGTTTGA GGCAGTCGAA 
CAGCAGGACA TGGATGCTGT GCAGATCCTC CTGTATCAGT ACACACCAGA AGAACTTGAC 
CTCAACACAC CTAACAGCGA GGGCTTGACA CCCCTGGATA TTGCCATCAT GACCAACAAT 
GTGCCCATTG CAAGGATTCT TCTGAGGACA GGGGCCCGAG AAAGTCCACA CTTTGTCAGC 
CTGGAAAGCC GAGCAATGCA CCTCAACACA CTGGTCCAGG AAGCCCAGGA GAGGGTGAGT 
GAACTGTCTG CCCAGGTGGA GAATGAAGGA TTCACTCTGG ACAACACAGA GAAAGAGAAG 
CAGCTGAAAG CTTGGGAGTG GAGGTATCGG CTCTACAGAC GCATGAAAAC AGGCTTTGAG 
CATGCCAGAG CCCCTGAGAT GCCAACCAAT GTCTGTCTCA TGGTAACCAG CAGCACATCA 
CTCACTGTCA GCTTCCAAGA GCCTCTTAGC GTCAATGCAG CTGTAGTAAC CAGGTATAAA 
GTGGAATGGA GTATGTCCGA AGACTTTTCT CCTTTGGCTG GAGAAATCAT CATGGATAAT 
CTGCAGACTC TGAGATGCAC AATCACAGGA CTTACAATGG GCCAACAGTA TTTTGTTCAA 
GTCTCGGCTT ACAATATGAA AGGATGGGGA CCTGCTCAGA CCACGACACC GGCATGTGCC 
TCTCCTTCTA ACTGGAAAGA CTATGACGAC AGAGAGCCCA GACACAAGGG ACAGAGTGAA 
GTTTTGGAAG GTCTGCTGCA GCAGGTCCGA GCCCTTCATC AGCATTACAG TTGCCGGGAA 
AGCACAAAAT TACAAACCAC AGGCCGCAAG CAGTCAGTCT CAAGAAGCCT GAAACACCTG 
TTCCATTCCT CGAACAAGTT TGTGAAGACC TTAAAACGGG GACTCTACAT AGCCGTTATA 
TTTTATTACA AAGACAATAT CTTAGTCACC AATGAAGATC AAGTACCAAT TGTTGAAATA 
GATGACTCTC ACACCAGTTC TATTACACAA GATTTTCTGT GGTTCACGAA GCTGTCTTGT 
ATGTGGGAAG ATATAAGGTG GCTGAGGCAA AGCATACCAA TATCCTCATC CTCATCCACA 
GTGCTGCAAA CTCGGCAGAA GATGCTCGCA GCAACAGCAC AGCTACAGAA TTTACTTGGG 
ACACACAACT TGGGAAGAGT TTACTATGAG CCCATTAAAG ATCGACATGG AAACATACTC 
ATAGTCACCA TCAGGGAGGT GGAGATGCTT TATTCATTTT TTAATGGCAA ATGGATGCAG 
ATCTCAAAGC TGCAAAGCCA GAGAAAGTCT CTATCAACAC CTGAGGAGCC AACAGCTTTA 
GACATTCTAC TGATAACCAT CCAGGATATT CTATCCTATC ACAAAAGGAG TCATCAGCGT 
CTCTTTCCTG GATTATATCT GGGTTACCTA AAGCTCTGTA GCTCTGTGGA TCAAATCAAA 
GTTCTTGTTA CCCAAAAGTT GCCCAACATT CTCTGCCACG TGAAGATCCG TGAAAACAAT 
AATATTTCTA GAGAGGAATG GGAATGGATC CAAAAGCTTT CTGGCTCTGA ATCTATGGAA 
AGTGTGGATC ATACTTCTGA CTGCCCCATG CAATTGTTCT TCTACGAGCT CCAGATGGCA 
GTGAAAGCTC TCCTTCAGCA GATCAATATA CCTCTACACC AGGCAAGGAA CTTCCGCCTC 
TACACACAGG AGGTGTTGGA AATGGGTCAC AATGTGTCCT TTCTTCTCCT GCTCCCTGCC 
TCAGACGACG TCTGTACAGC CCCAGGACAG AATAATCCTT ACACCCCACA CTCAGGGTTT 
CTTAACCTCC CTCTTCAGAT GTTTGAACTT GTTCATTTTT GCAGCTACAG AGAGAAATTT 
ATTAGTCTGT ATTGCCGCCT TTCTGCTGTT GTGGAGCTGG ATTCTCTGAA CACCCAACAG 
TCCCTCAGGG AAGCAATCTC AGACAGCGAG GTTGCAGCTG CCAAACAAAG ACACCAGCAA 
GTTTTAGATT TCATTCAGCA AATAGATGAA GTCTGGCGTG AAATGAGATG GATCATGGAT 
GCTCTACAGT ATGCAAGATA CAAACAACCA GTTTCTGGCT TGCCCATCAC TAAGCTGATA 
GACCCCTCAG ATGAGCAGAG CCTAAAGAAG ATCAATTCTA CATCATCATC ACATATAGAC 
TGTCTTCCAT CCCCACCCCC ATCCCCAGAG ATGCACAGAA GAAAGACAGT GAGTGATTCA 
CAGCCCTGCT CTGATGAAGA AGCCTGCTCA GAAGTCTTCC TCCCCACCAA CAGTGACTAC 
GACTCCAGCG ATGCCCTGAG CCCCAGAGAC CTGGACCTGG TCTACCTATC ATCTCACGAC 
ATTGCGCAGC AGACCCTTAG CGGCCTAAGC GGCAGCGCCC CCGACGTCCT GCAAGTGCAC 
GACGTGAAAA CCCCTCTGGG GCCGGGCCAG GATCCCCAGG GCGAGGGCCC AAATCCCGAT 
CACTCATGTG CCGAGTTTCT CCATAGCCTG ACCCTCACGG GGTTCACACC CAAGAACCAC 
GCCAAGACTG TGTCCGGTGG GCGGCCCCCG CTAGGCTTCC TGGGAAAGCG GAAGCCAGGC 
AAGCACCCCC ACTATGGCGG CTTCAGCCGC CATCATCGCT GGTTGCGCAT CCACAGCGAG 
ACCCAGTCGC TATCGCTCTC TGAGGGCATT TATACACAGC ACCTGTCCCA GGCCTGTGGT 
CTGGCCCAGG AGCCCAAGGA GGCCAAGCGG GCCGGCCCTG CCCTTGATGA TCCCAGGGGC 
CTAACTCTGG CCCACGCTGC CAGCCTTCCT GAGGAGCGGA ACAGCAGTCT CCAGGACGCG 
AGGCCTTCCG TCCGCCGCCT CTACGTGGAG CCCTACGCAG CGGCCGTGGT GGCCCAGGAC 
GAAAAACCAT GGGCAAGCTT GAGCCCGCCC TCTGGAGGCC GCATCACCCT GCCCAGCCCC 
ACTGGCCCCG ATGTGAGTCA GGAGGGCCCC ACCGCCTCTC CCATGTCAGA AATACTCAGC 
AGCATGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ANKFN1
Entrez Gene ID	162282
Full Name	ankyrin repeat and fibronectin type III domain containing 1
General protein information	Preferred Names ankyrin repeat and fibronectin type III domain containing 1 Names ankyrin repeat and fibronectin type-III domain-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q22

Pan troglodytes (chimpanzee)	ANKFN1	XP_511898.4
Danio rerio (zebrafish)	LOC100333998	XP_003201826.2
Danio rerio (zebrafish)	LOC557615	XP_003199674.2
Drosophila melanogaster (fruit fly)	CG6954	NP_001247244.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001726	XP_321358.5
	LOC707235	XP_002808169.1
Homo sapiens (human)	ANKFN1	NP_694960.2
Canis lupus familiaris (dog)	ANKFN1	XP_548219.3
Bos taurus (cattle)	ANKFN1	XP_003587422.2
Mus musculus (house mouse)	Ankfn1	NP_001074402.1
Gallus gallus (chicken)	ANKFN1	XP_004946219.1
Xenopus tropicalis (tropical clawed frog)	LOC100490095	XP_004918819.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ANKFN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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