SI:CH211-277E21.2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-277E21.2
Entrez Gene ID	100333998
Full Name	si:ch211-277e21.2
Synonyms	ankfn1
General protein information	Preferred Names si:ch211-277e21.2 Names ankyrin repeat and fibronectin type-III domain-containing protein 1 ankyrin-repeat and fibronectin type III domain containing 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306802.3	XP_009305077.2	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X3
XM_009306801.2	XP_009305076.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X2
XM_021480370.1	XP_021336045.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_009306800.2	XP_009305075.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
XM_021472578.1	XP_021328253.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X2
XM_009306802.2	XP_009305077.1	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANKFN1	XP_511898.4
Danio rerio (zebrafish)	LOC100333998	XP_003201826.2
Danio rerio (zebrafish)	LOC557615	XP_003199674.2
Drosophila melanogaster (fruit fly)	CG6954	NP_001247244.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001726	XP_321358.5
	LOC707235	XP_002808169.1
Homo sapiens (human)	ANKFN1	NP_694960.2
Canis lupus familiaris (dog)	ANKFN1	XP_548219.3
Bos taurus (cattle)	ANKFN1	XP_003587422.2
Mus musculus (house mouse)	Ankfn1	NP_001074402.1
Gallus gallus (chicken)	ANKFN1	XP_004946219.1
Xenopus tropicalis (tropical clawed frog)	LOC100490095	XP_004918819.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SI:CH211-277E21.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-277E21.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145403	XM_021472578.1 Latest version!	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa146660	XM_009306802.3 Latest version!	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa49905	XM_009306802.2 Latest version!	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49903	XM_009306800.2 Latest version!	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa49903	XM_021480370.1 Latest version!	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa49904	XM_009306801.2 Latest version!	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-277E21.2 ( XM_021472578.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328253.1 Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145403
Clone ID Related Accession (Same CDS sequence)	XM_021472578.1
Accession Version	XM_021472578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394854.1) and transcript sequence (GFIL01023045.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 584 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACAGGGT TCATTTACTT CCTGATGGCG AACAGCGATC ACATTTGTTT CAGGTTTTTA 
CGGGAAGAGG ATGCAGATGT TCTGAGGATC CGCCGCGGTT CCATGAGTCC AGAACCGGGG 
AACAAAGAGA AATGGCTCAA GATGTCTTAT GAGGCCGAGA AAACTTGCCT GTTGGCTCCA 
CAGGAATCAC AAACACCATG TGTCTGCCAC ACAGCTAATG AGAAATATGA AATCCCAGGA 
ATGGGAGACG ACAGTGTTTT AGAGATGCTA AGCTACTCCA AATTCTCGGA TCTGGAGACG 
TGGCTCTGTA TGCCATCGAC TTTGTTACCC AGAAGCAGGG ATTCAGTTTG CTCCCTGCCA 
CCACCATCCC ATGAGAACGG CACTGCCGAC ACAGACAGAG AATCCAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTTGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGATGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG GATGCGCACA 
GGATTCGAGC ATGCACGGCC GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGAC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG ACGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACAGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GATTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTA 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGCGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAAGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC AGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCAGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328253.1
CDS	178..4416
Translation	MTGFIYFLMANSDHICFRFLREEDADVLRIRRGSMSPEPGNKEKWLKMSYEAEKTCLLAPQESQTPCVCHTANEKYEIPGMGDDSVLEMLSYSKFSDLETWLCMPSTLLPRSRDSVCSLPPPSHENGTADTDRESRPPENTSINLSQCQERKTHLLPPSSLMPVSASPTSSTPLRTTSTPLPKPNRDSGQGGVSVRKRRRLAASPGGLHWDASGSVLRDHDRGETPSLSEVKRFPRSVDSESLVPELWRDRAPLRKTISIDDRLLQQTPREHHRLLSRLERGKKKLRNINSLGATGRYETHKKSESRISRLAQRLNQRQSDAALIKDFRPLFLLSGSAGSSQSLDRNFSISMSQQMQNLQLTQSKKGAGPASPSAAKRLYRNLSEKFKGSHSSFEDAYFFGRSDRIRKVSNIQSSEALFEAVEQQDLDAVQILLFQYTADELDLNTPNSEGLTPLDISIMTNNVPIAKLLLKAGGKESPHFVSLESRDAHLSALVQEAQRRASELSNQVMRESLSLETSDKEKQLKAWEWRCKLYKRMRTGFEHARPPEAPLMVRLSVTGSTTLTVSFQEPASMNSAVVTKYKVEWSCLKDFSLLAGELILENLQSLKCTITGLTMGRQYYVQVSAYNMKGWGPAQLSQPPSAVPSNWKDCDGRESRRRGHIEAMERLLQQVRATHQHYCCGDTSKLQNPSRKQSVSRSLKHLFHSSTKFVKSLKRGVYIASVFYHKDSLLVTNEDQIPIVEVDDSYSSSLMQDFLWFTKLSCMWEDVRWLRQSLAVSTSSSSTLQSRQKMLAAAGQLQNLLGTHNLGRVHYEPIKDRHGNVLLVTVREMDSLYSFFNGKWMQVSKLQSQRKSLSTPEEPYALDILLITIQDILAYQRRSQHRLSSGLYLGYLKLSSSVDQIKVLVPQRMPNMLCHTKIRDNWNVSRDEWEWLQSLSGPVEVERADQATDCLLFSELQTAIKSLLHQINLPLHQAKHFRLYTHEVLELGHNVSFLLLLPASDDVCSAPGQTNPYTPHSGFLNLPLQMFELVHFCSYKEKFISLYCRLSSVLDLDALITQQAFREAITDSEVSTAKQRHQHILDYIQQLDEMWRDVRWITNALQYARYKQPLGWVPITWLVDVSVEPPVQKNDSTSSNTDYVPTPSPSPEMRRRKPTIESQPGSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYSSAQDLSHQAVHVLSGSAPDVLQMHDLKYSVCSKSILETESCTKDLEDLSLSSYSVKTTDKPSRSKFLADAPTKRKLLSKSHPQRSYFGGPHRWLRVQSESHTPSLSEGIYTRQSDIDLPLETPLSIPHSPTTSYSLDEYRQPYRESKPNVRRIFVESCSKTSPCRDAPHWEEEEEKGSRGATASGARPGYSSTSEAQDVDSDEQTNEQVSEILSSTL

Target ORF information:

RefSeq Version	XM_021472578.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472578.1

ORF Insert Sequence:

ATGACAGGGT TCATTTACTT CCTGATGGCG AACAGCGATC ACATTTGTTT CAGGTTTTTA 
CGGGAAGAGG ATGCAGATGT TCTGAGGATC CGCCGCGGTT CCATGAGTCC AGAACCGGGG 
AACAAAGAGA AATGGCTCAA GATGTCTTAT GAGGCCGAGA AAACTTGCCT GTTGGCTCCA 
CAGGAATCAC AAACACCATG TGTCTGCCAC ACAGCTAATG AGAAATATGA AATCCCAGGA 
ATGGGAGACG ACAGTGTTTT AGAGATGCTA AGCTACTCCA AATTCTCGGA TCTGGAGACG 
TGGCTCTGTA TGCCATCGAC TTTGTTACCC AGAAGCAGGG ATTCAGTTTG CTCCCTGCCA 
CCACCATCCC ATGAGAACGG CACTGCCGAC ACAGACAGAG AATCCAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTTGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGATGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG GATGCGCACA 
GGATTCGAGC ATGCACGGCC GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGAC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG ACGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACAGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GATTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTA 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGCGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAAGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC AGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCAGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-277E21.2 ( XM_009306802.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305077.2 Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa146660
Clone ID Related Accession (Same CDS sequence)	XM_009306802.3
Accession Version	XM_009306802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395303.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA TGGAACGGAT TGTTCAGTAT TTGTTCAGAA GAAAAAAGTC AGGATCTCAC 
AAGAGAGGAT CATGGAAAAG CCAAAGCTAT ACGCTTCCAG ATTCAAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTGGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGACGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG CATGCGCACA 
GGATTCGAGC ATGCACGGCC GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGAC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG ACGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACAGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GATTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTA 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGTGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAGGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC GGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCGGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305077.2
CDS	336..4274
Translation	MFAMERIVQYLFRRKKSGSHKRGSWKSQSYTLPDSRPPENTSINLSQCQERKTHLLPPSSLMPVSASPTSSTPLRTTSTPLPKPNRDSGQGGVSVRKRRRLAASPGGLHWDASGSVLRDHDRGETPSLSEVKRFPRSVDSESLVPELWRDRAPLRKTISIDDRLLQQTPREHHRLLSRLERGKKKLRNINSLGATGRYETHKKSESRISRLAQRLNQRQSDAALIKDFRPLFLLSGSAGSSQSLDRNFSISMSQQMQNLQLTQSKKGAGPASPSAAKRLYRNLSEKFKGSHSSFEDAYFFGRSDRIRKVSNIQSSEALFEAVEQQDLDAVQILLFQYTADELDLNTPNSEGLTPLDISIMTNNVPIAKLLLKAGGKESPHFVSLESRDAHLSALVQEAQRRASELSNQVMRESLSLETSDKEKQLKAWEWRCKLYKRMRTGFEHARPPEAPLMVRLSVTGSTTLTVSFQEPASMNSAVVTKYKVEWSCLKDFSLLAGELILENLQSLKCTITGLTMGRQYYVQVSAYNMKGWGPAQLSQPPSAVPSNWKDCDGRESRRRGHIEAMERLLQQVRATHQHYCCGDTSKLQNPSRKQSVSRSLKHLFHSSTKFVKSLKRGVYIASVFYHKDSLLVTNEDQIPIVEVDDSYSSSLMQDFLWFTKLSCMWEDVRWLRQSLAVSTSSSSTLQSRQKMLAAAGQLQNLLGTHNLGRVHYEPIKDRHGNVLLVTVREMDSLYSFFNGKWMQVSKLQSQRKSLSTPEEPYALDILLITIQDILAYQRRSQHRLSSGLYLGYLKLSSSVDQIKVLVPQRMPNMLCHTKIRDNWNVSRDEWEWLQSLSGPVEVERADQATDCLLFSELQTAIKSLLHQINLPLHQAKHFRLYTHEVLELGHNVSFLLLLPASDDVCSAPGQTNPYTPHSGFLNLPLQMFELVHFCSYKEKFISLYCRLSSVLDLDALITQQAFREAITDSEVSTAKQRHQHILDYIQQLDEMWRDVRWITNALQYARYKQPLGWVPITWLVDVSVEPPVQKNDSTSSNTDYVPTPSPSPEMRRRKPTIESQPGSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYSSAQDLSHQAVHVLSGSAPDVLQMHDLKYSVCSKSILETESCTKDLEDLSLSSYSVKTTDKPSRSKFLADAPTKRKLLSKSHPQRSYFGGPHRWLRVQSESHTPSLSEGIYTRQSDIDLPLETPLSIPHSPTTSYSLDEYRQPYRESKPNVRRIFVESCSKTSPCRDAPHWEEEEEKGSRGATASGARPGYSSTSEAQDVDSDEQTNEQVSEILSSTL

Target ORF information:

RefSeq Version	XM_009306802.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306802.3

ORF Insert Sequence:

ATGTTTGCAA TGGAACGGAT TGTTCAGTAT TTGTTCAGAA GAAAAAAGTC AGGATCTCAC 
AAGAGAGGAT CATGGAAAAG CCAAAGCTAT ACGCTTCCAG ATTCAAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTGGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGACGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG CATGCGCACA 
GGATTCGAGC ATGCACGGCC GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGAC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG ACGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACAGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GATTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTA 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGTGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAGGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC GGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCGGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-277E21.2 ( XM_009306802.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305077.1 Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X3, mRNA.

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CloneID	ODa49905
Clone ID Related Accession (Same CDS sequence)	XM_009306802.2
Accession Version	XM_009306802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA TGGAACGGAT TGTTCAGTAT TTGTTCAGAA GAAAAAAGTC AGGATCTCAC 
AAGAGAGGAT CATGGAAAAG CCAAAGCTAT ACGCTTCCAG ATTCAAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTGGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGACGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG CATGCGCACA 
GGATTCGAGC ATGCACGGCA GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGGC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG AAGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACGGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GACTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTG 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGTGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAGGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC GGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCGGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305077.1
CDS	304..4242
Translation	MFAMERIVQYLFRRKKSGSHKRGSWKSQSYTLPDSRPPENTSINLSQCQERKTHLLPPSSLMPVSASPTSSTPLRTTSTPLPKPNRDSGQGGVSVRKRRRLAASPGGLHWDASGSVLRDHDRGETPSLSEVKRFPRSVDSESLVPELWRDRAPLRKTISIDDRLLQQTPREHHRLLSRLERGKKKLRNINSLGATGRYETHKKSESRISRLAQRLNQRQSDAALIKDFRPLFLLSGSAGSSQSLDRNFSISMSQQMQNLQLTQSKKGAGPASPSAAKRLYRNLSEKFKGSHSSFEDAYFFGRSDRIRKVSNIQSSEALFEAVEQQDLDAVQILLFQYTADELDLNTPNSEGLTPLDISIMTNNVPIAKLLLKAGGKESPHFVSLESRDAHLSALVQEAQRRASELSNQVMRESLSLETSDKEKQLKAWEWRCKLYKRMRTGFEHARQPEAPLMVRLSVTGSTTLTVSFQEPASMNSAVVTKYKVEWSCLKDFSLLAGELILENLQSLKCTITGLTMGRQYYVQVSAYNMKGWGPAQLSQPPSAVPSNWKDCDGRESRRRGHIEAMERLLQQVRATHQHYCCGDTSKLQNPSRKQSVSRSLKHLFHSSTKFVKSLKRGVYIASVFYHKDSLLVTNEDQIPIVEVDDSYSSSLMQDFLWFTKLSCMWEDVRWLRQSLAVSTSSSSTLQSRQKMLAAAGQLQNLLGTHNLGRVHYEPIKDRHGNVLLVTVREMDSLYSFFNGKWMQVSKLQSQRKSLSTPEEPYALDILLITIQDILAYQRRSQHRLSSGLYLGYLKLSSSVDQIKVLVPQRMPNMLCHTKIRDNWNVSRDEWEWLQSLSGPVEVERADQATGCLLFSELQTAIKSLLHQINLPLHQAKHFRLYTHEVLELGHNVSFLLLLPASDDVCSAPGQTNPYTPHSGFLNLPLQMFELVHFCSYKEKFISLYCRLSSVLDLDALITQQAFREAITDSEVSTAKQRHQHILDYIQQLDEMWREVRWITNALQYARYKQPLGWVPITWLVDVSVEPPVQKNDSTSSNTDYVPTPSPSPEMRRRKPTIESQPGSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYSSAQDLSHQAVHVLSGSAPDVLQMHDLKYSVCSKSILETESCTKDLEDLSLSSYSVKTTDKPSRSKFLADAPTKRKLLSKSHPQRSYFGGPHRWLRVQSESHTPSLSEGIYTRQSDIDLPLETPLSIPHSPTTSYSLDEYRQPYRESKPNVRRIFVESCSKTSPCRDAPHWEEEEEKGSRGATASGARPGYSSTSEAQDVDSDEQTNEQVSEILSSTL

Target ORF information:

RefSeq Version	XM_009306802.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306802.2

ORF Insert Sequence:

ATGTTTGCAA TGGAACGGAT TGTTCAGTAT TTGTTCAGAA GAAAAAAGTC AGGATCTCAC 
AAGAGAGGAT CATGGAAAAG CCAAAGCTAT ACGCTTCCAG ATTCAAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTGGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGACGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG CATGCGCACA 
GGATTCGAGC ATGCACGGCA GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGGC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG AAGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACGGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GACTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTG 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGTGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAGGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC GGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCGGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-277E21.2 ( XM_021480370.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336045.1 Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X1, mRNA.

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CloneID	ODa49903
Clone ID Related Accession (Same CDS sequence)	XM_021480370.1 , XM_009306800.2
Accession Version	XM_021480370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCGC AGAAGTTTTC ACCACGCCGA AGTGGAAGAC ACGTAACGGG AAGTTTTGAG 
TCCAGTTTCA GGTTTTTACG GGAAGAGGAT GCAGATGTTC TGAGGATCCG CCGCGGTTCC 
ATGAGTCCAG AACCGGAGAA CAAAGAGAAA TGGCTCAAGA TGTCTTATGA GGCCGAGAAA 
ACTTGCCTGT TGGCTCCACA GGAATCACAA ACACCATGTG TCTGCCACAC AGCTAATGAG 
AAATATGAAA TCCCTGGAAT GGGAGACGAC AGTGTTTTAG AGATGCTAAG CTACTCCAAA 
TTCTCGGATC TGGAGACGTG GCTCTGTATG CCATCGACTT TGTTACCCAG AAGCAGGGAT 
TCAGTTTGCT CCCTGCCACC ACCATCCCAT GAGAACGGCA CTGCCGACAC AGACAGAGAA 
TCCAGGCCCC CGGAGAACAC GTCTATCAAT CTGAGCCAGT GCCAGGAGAG GAAAACCCAT 
CTATTGCCAC CCTCATCGCT CATGCCAGTC TCAGCCTCCC CTACATCCTC AACTCCCCTC 
CGGACCACCT CAACTCCTCT GCCTAAACCC AACAGAGACT CCGGTCAGGG CGGTGTGAGT 
GTTCGCAAAC GCCGGCGTCT GGCTGCTAGC CCTGGAGGAC TGCACTGGGA TGCATCAGGA 
TCAGTGCTGC GTGACCATGA CCGAGGGGAG ACGCCGTCAC TGTCGGAAGT GAAGCGCTTC 
CCTAGAAGTG TGGATTCGGA GAGCCTCGTT CCAGAACTCT GGAGGGACCG AGCACCTCTT 
AGGAAGACCA TTTCTATCGA TGACCGCCTT CTCCAGCAAA CGCCCAGAGA ACATCACAGA 
CTCCTCAGCC GACTGGAGAG AGGCAAGAAG AAACTCAGGA ACATAAATAG TCTTGGAGCC 
ACTGGCAGAT ACGAAACCCA TAAAAAATCC GAGAGCAGGA TCTCCCGTCT GGCTCAGAGA 
CTCAACCAGA GGCAGAGCGA TGCGGCATTG ATTAAGGATT TCAGACCTCT TTTCCTCCTC 
TCTGGATCTG CTGGAAGCTC CCAGAGTCTG GACAGGAACT TCTCCATCAG TATGAGCCAG 
CAGATGCAGA ACCTGCAGCT GACGCAGAGC AAGAAGGGGG CTGGTCCCGC CTCCCCCAGC 
GCTGCCAAAC GCCTCTACCG GAACCTCTCC GAGAAGTTTA AAGGGAGCCA CTCATCCTTC 
GAGGATGCCT ACTTTTTCGG CCGATCCGAC CGTATACGCA AAGTATCAAA CATCCAGAGC 
AGCGAGGCTC TGTTCGAGGC TGTTGAACAG CAGGATCTGG ATGCCGTTCA GATCCTGCTG 
TTTCAGTACA CAGCTGATGA ACTGGACCTG AACACCCCCA ATAGTGAAGG CCTGACGCCT 
CTGGACATCT CCATCATGAC CAATAACGTT CCCATCGCCA AACTGTTGCT TAAAGCTGGA 
GGGAAGGAGA GCCCTCACTT TGTCAGTTTG GAGAGCAGAG ACGCTCACCT CTCAGCACTA 
GTCCAGGAAG CCCAAAGGAG AGCCAGTGAA CTGTCCAACC AGGTGATGAG AGAAAGTCTC 
AGCCTGGAAA CGTCTGACAA AGAAAAACAG CTCAAAGCTT GGGAGTGGCG ATGCAAACTC 
TACAAGCGCA TGCGCACAGG ATTCGAGCAT GCACGGCAGC CGGAGGCTCC ATTGATGGTG 
CGTCTGAGTG TGACCGGCAG CACGACTCTC ACTGTTAGCT TTCAGGAACC TGCCAGCATG 
AATTCAGCTG TGGTGACCAA ATACAAAGTT GAATGGAGCT GTCTGAAGGA CTTCTCTTTG 
TTAGCCGGAG AACTGATCCT TGAGAATCTG CAGTCCCTCA AATGCACAAT TACAGGCCTC 
ACCATGGGTA GGCAGTACTA TGTTCAAGTC TCTGCTTACA ATATGAAGGG TTGGGGTCCA 
GCACAACTTT CTCAGCCTCC TTCAGCTGTT CCCTCCAACT GGAAGGACTG TGATGGGAGA 
GAAAGCAGGA GGAGAGGTCA CATCGAGGCC ATGGAGAGGC TCTTACAGCA GGTTCGAGCC 
ACACACCAGC ACTACTGCTG TGGAGACACA TCAAAGTTGC AGAATCCAAG TCGTAAACAG 
TCTGTGTCCC GGAGCCTGAA GCACCTTTTT CACTCCTCTA CGAAATTTGT CAAGAGTCTT 
AAAAGGGGAG TTTATATAGC GTCAGTATTT TACCATAAGG ACAGTCTGCT AGTGACTAAT 
GAGGATCAGA TCCCCATAGT GGAGGTGGAC GATTCATACA GCAGCTCCCT CATGCAGGAC 
TTTCTGTGGT TCACCAAGCT TTCTTGTATG TGGGAGGACG TCCGGTGGCT CAGACAAAGC 
CTGGCAGTCT CCACCTCATC CTCTTCCACT CTGCAGTCCA GACAGAAGAT GCTGGCGGCC 
GCGGGACAAC TGCAGAATCT TCTGGGCACT CACAACCTGG GTCGAGTCCA TTATGAGCCC 
ATTAAAGATC GTCATGGAAA TGTGCTGCTT GTGACGGTGA GGGAAATGGA CAGTCTTTAC 
TCCTTCTTCA ACGGGAAGTG GATGCAAGTC TCCAAACTGC AGAGTCAGAG GAAGTCCCTG 
TCTACACCTG AGGAACCATA TGCCCTCGAC ATCCTACTCA TCACCATACA GGACATTCTG 
GCGTATCAGA GACGGAGTCA GCACCGGCTG TCCTCTGGCC TTTATCTGGG TTACCTGAAA 
CTCAGCAGCT CTGTGGATCA GATCAAGGTT CTGGTTCCTC AGCGGATGCC CAACATGCTT 
TGCCACACCA AGATACGAGA CAACTGGAAC GTGTCAAGGG ATGAGTGGGA ATGGCTGCAG 
TCACTGTCTG GCCCTGTGGA GGTGGAAAGA GCAGATCAGG CTACAGGCTG CCTCCTTTTC 
TCTGAGCTCC AGACAGCCAT TAAAAGTCTG CTGCACCAGA TTAATCTACC TCTCCACCAG 
GCCAAGCACT TCCGTCTCTA CACACACGAG GTGCTGGAGC TGGGTCACAA TGTGTCCTTT 
CTTCTGCTGC TGCCCGCCTC AGACGACGTC TGCTCTGCCC CGGGACAGAC CAACCCCTAC 
ACACCACACT CGGGGTTCCT CAACCTCCCT CTTCAAATGT TTGAGCTCGT TCATTTCTGC 
TCGTATAAGG AGAAATTCAT CAGTCTTTAC TGCCGGCTGT CCTCAGTGCT GGATCTGGAT 
GCTCTGATTA CCCAGCAGGC TTTTAGAGAG GCCATCACTG ACTCAGAGGT GTCCACTGCC 
AAACAGAGGC ACCAGCACAT TCTGGACTAC ATTCAGCAAT TGGACGAGAT GTGGCGAGAA 
GTCCGCTGGA TCACCAATGC GCTGCAGTAC GCCCGCTATA AACAGCCTCT CGGTTGGGTG 
CCGATCACCT GGCTAGTAGA TGTCAGCGTG GAGCCTCCTG TCCAGAAGAA TGACTCCACG 
TCGTCTAACA CGGACTACGT GCCCACTCCC TCACCCTCTC CAGAAATGAG AAGAAGAAAG 
CCCACTATTG AGTCCCAGCC AGGCTCAGAT GAAGAAGGCT GCTCAGAAGT TTTCCTTCCC 
ACTGACAGTG ACTATGACTC CAGTGATGCC CTGAGCCCTC GAGACCTAGA CTTAGTTTAT 
TCCTCAGCCC AGGATCTCTC CCATCAGGCC GTGCATGTCC TCAGTGGCAG CGCTCCCGAT 
GTCCTCCAAA TGCACGATCT TAAATACAGC GTCTGCTCCA AGTCGATCCT GGAGACTGAA 
TCCTGCACAA AAGATTTGGA GGATCTATCG CTCTCCTCTT ACTCCGTGAA GACCACCGAC 
AAACCGTCCC GCTCCAAGTT TCTTGCCGAT GCCCCAACAA AACGCAAGCT GCTGTCCAAA 
AGCCATCCTC AGAGGAGTTA TTTCGGCGGA CCACACCGCT GGCTTCGTGT ACAAAGCGAA 
AGCCACACTC CGTCGCTTTC CGAAGGTATT TACACTAGAC AGAGTGATAT CGACCTCCCG 
CTGGAGACAC CACTTTCAAT ACCGCACTCG CCCACTACCT CCTACAGTCT GGATGAGTAC 
AGGCAGCCCT ACAGAGAATC CAAACCCAAC GTTCGCAGAA TATTTGTAGA GTCATGCAGC 
AAGACTTCAC CCTGTAGAGA TGCACCGCAT TGGGAGGAAG AGGAGGAGAA AGGCTCACGA 
GGAGCAACGG CGTCGGGGGC TCGTCCGGGA TACAGCTCAA CATCGGAAGC TCAGGATGTG 
GATTCAGATG AGCAAACTAA TGAACAAGTG TCGGAGATCC TCAGCAGCAC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336045.1
CDS	499..4755
Translation	MLSQKFSPRRSGRHVTGSFESSFRFLREEDADVLRIRRGSMSPEPENKEKWLKMSYEAEKTCLLAPQESQTPCVCHTANEKYEIPGMGDDSVLEMLSYSKFSDLETWLCMPSTLLPRSRDSVCSLPPPSHENGTADTDRESRPPENTSINLSQCQERKTHLLPPSSLMPVSASPTSSTPLRTTSTPLPKPNRDSGQGGVSVRKRRRLAASPGGLHWDASGSVLRDHDRGETPSLSEVKRFPRSVDSESLVPELWRDRAPLRKTISIDDRLLQQTPREHHRLLSRLERGKKKLRNINSLGATGRYETHKKSESRISRLAQRLNQRQSDAALIKDFRPLFLLSGSAGSSQSLDRNFSISMSQQMQNLQLTQSKKGAGPASPSAAKRLYRNLSEKFKGSHSSFEDAYFFGRSDRIRKVSNIQSSEALFEAVEQQDLDAVQILLFQYTADELDLNTPNSEGLTPLDISIMTNNVPIAKLLLKAGGKESPHFVSLESRDAHLSALVQEAQRRASELSNQVMRESLSLETSDKEKQLKAWEWRCKLYKRMRTGFEHARQPEAPLMVRLSVTGSTTLTVSFQEPASMNSAVVTKYKVEWSCLKDFSLLAGELILENLQSLKCTITGLTMGRQYYVQVSAYNMKGWGPAQLSQPPSAVPSNWKDCDGRESRRRGHIEAMERLLQQVRATHQHYCCGDTSKLQNPSRKQSVSRSLKHLFHSSTKFVKSLKRGVYIASVFYHKDSLLVTNEDQIPIVEVDDSYSSSLMQDFLWFTKLSCMWEDVRWLRQSLAVSTSSSSTLQSRQKMLAAAGQLQNLLGTHNLGRVHYEPIKDRHGNVLLVTVREMDSLYSFFNGKWMQVSKLQSQRKSLSTPEEPYALDILLITIQDILAYQRRSQHRLSSGLYLGYLKLSSSVDQIKVLVPQRMPNMLCHTKIRDNWNVSRDEWEWLQSLSGPVEVERADQATGCLLFSELQTAIKSLLHQINLPLHQAKHFRLYTHEVLELGHNVSFLLLLPASDDVCSAPGQTNPYTPHSGFLNLPLQMFELVHFCSYKEKFISLYCRLSSVLDLDALITQQAFREAITDSEVSTAKQRHQHILDYIQQLDEMWREVRWITNALQYARYKQPLGWVPITWLVDVSVEPPVQKNDSTSSNTDYVPTPSPSPEMRRRKPTIESQPGSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYSSAQDLSHQAVHVLSGSAPDVLQMHDLKYSVCSKSILETESCTKDLEDLSLSSYSVKTTDKPSRSKFLADAPTKRKLLSKSHPQRSYFGGPHRWLRVQSESHTPSLSEGIYTRQSDIDLPLETPLSIPHSPTTSYSLDEYRQPYRESKPNVRRIFVESCSKTSPCRDAPHWEEEEEKGSRGATASGARPGYSSTSEAQDVDSDEQTNEQVSEILSSTL

Target ORF information:

RefSeq Version	XM_021480370.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480370.1

ORF Insert Sequence:

ATGCTGTCGC AGAAGTTTTC ACCACGCCGA AGTGGAAGAC ACGTAACGGG AAGTTTTGAG 
TCCAGTTTCA GGTTTTTACG GGAAGAGGAT GCAGATGTTC TGAGGATCCG CCGCGGTTCC 
ATGAGTCCAG AACCGGAGAA CAAAGAGAAA TGGCTCAAGA TGTCTTATGA GGCCGAGAAA 
ACTTGCCTGT TGGCTCCACA GGAATCACAA ACACCATGTG TCTGCCACAC AGCTAATGAG 
AAATATGAAA TCCCTGGAAT GGGAGACGAC AGTGTTTTAG AGATGCTAAG CTACTCCAAA 
TTCTCGGATC TGGAGACGTG GCTCTGTATG CCATCGACTT TGTTACCCAG AAGCAGGGAT 
TCAGTTTGCT CCCTGCCACC ACCATCCCAT GAGAACGGCA CTGCCGACAC AGACAGAGAA 
TCCAGGCCCC CGGAGAACAC GTCTATCAAT CTGAGCCAGT GCCAGGAGAG GAAAACCCAT 
CTATTGCCAC CCTCATCGCT CATGCCAGTC TCAGCCTCCC CTACATCCTC AACTCCCCTC 
CGGACCACCT CAACTCCTCT GCCTAAACCC AACAGAGACT CCGGTCAGGG CGGTGTGAGT 
GTTCGCAAAC GCCGGCGTCT GGCTGCTAGC CCTGGAGGAC TGCACTGGGA TGCATCAGGA 
TCAGTGCTGC GTGACCATGA CCGAGGGGAG ACGCCGTCAC TGTCGGAAGT GAAGCGCTTC 
CCTAGAAGTG TGGATTCGGA GAGCCTCGTT CCAGAACTCT GGAGGGACCG AGCACCTCTT 
AGGAAGACCA TTTCTATCGA TGACCGCCTT CTCCAGCAAA CGCCCAGAGA ACATCACAGA 
CTCCTCAGCC GACTGGAGAG AGGCAAGAAG AAACTCAGGA ACATAAATAG TCTTGGAGCC 
ACTGGCAGAT ACGAAACCCA TAAAAAATCC GAGAGCAGGA TCTCCCGTCT GGCTCAGAGA 
CTCAACCAGA GGCAGAGCGA TGCGGCATTG ATTAAGGATT TCAGACCTCT TTTCCTCCTC 
TCTGGATCTG CTGGAAGCTC CCAGAGTCTG GACAGGAACT TCTCCATCAG TATGAGCCAG 
CAGATGCAGA ACCTGCAGCT GACGCAGAGC AAGAAGGGGG CTGGTCCCGC CTCCCCCAGC 
GCTGCCAAAC GCCTCTACCG GAACCTCTCC GAGAAGTTTA AAGGGAGCCA CTCATCCTTC 
GAGGATGCCT ACTTTTTCGG CCGATCCGAC CGTATACGCA AAGTATCAAA CATCCAGAGC 
AGCGAGGCTC TGTTCGAGGC TGTTGAACAG CAGGATCTGG ATGCCGTTCA GATCCTGCTG 
TTTCAGTACA CAGCTGATGA ACTGGACCTG AACACCCCCA ATAGTGAAGG CCTGACGCCT 
CTGGACATCT CCATCATGAC CAATAACGTT CCCATCGCCA AACTGTTGCT TAAAGCTGGA 
GGGAAGGAGA GCCCTCACTT TGTCAGTTTG GAGAGCAGAG ACGCTCACCT CTCAGCACTA 
GTCCAGGAAG CCCAAAGGAG AGCCAGTGAA CTGTCCAACC AGGTGATGAG AGAAAGTCTC 
AGCCTGGAAA CGTCTGACAA AGAAAAACAG CTCAAAGCTT GGGAGTGGCG ATGCAAACTC 
TACAAGCGCA TGCGCACAGG ATTCGAGCAT GCACGGCAGC CGGAGGCTCC ATTGATGGTG 
CGTCTGAGTG TGACCGGCAG CACGACTCTC ACTGTTAGCT TTCAGGAACC TGCCAGCATG 
AATTCAGCTG TGGTGACCAA ATACAAAGTT GAATGGAGCT GTCTGAAGGA CTTCTCTTTG 
TTAGCCGGAG AACTGATCCT TGAGAATCTG CAGTCCCTCA AATGCACAAT TACAGGCCTC 
ACCATGGGTA GGCAGTACTA TGTTCAAGTC TCTGCTTACA ATATGAAGGG TTGGGGTCCA 
GCACAACTTT CTCAGCCTCC TTCAGCTGTT CCCTCCAACT GGAAGGACTG TGATGGGAGA 
GAAAGCAGGA GGAGAGGTCA CATCGAGGCC ATGGAGAGGC TCTTACAGCA GGTTCGAGCC 
ACACACCAGC ACTACTGCTG TGGAGACACA TCAAAGTTGC AGAATCCAAG TCGTAAACAG 
TCTGTGTCCC GGAGCCTGAA GCACCTTTTT CACTCCTCTA CGAAATTTGT CAAGAGTCTT 
AAAAGGGGAG TTTATATAGC GTCAGTATTT TACCATAAGG ACAGTCTGCT AGTGACTAAT 
GAGGATCAGA TCCCCATAGT GGAGGTGGAC GATTCATACA GCAGCTCCCT CATGCAGGAC 
TTTCTGTGGT TCACCAAGCT TTCTTGTATG TGGGAGGACG TCCGGTGGCT CAGACAAAGC 
CTGGCAGTCT CCACCTCATC CTCTTCCACT CTGCAGTCCA GACAGAAGAT GCTGGCGGCC 
GCGGGACAAC TGCAGAATCT TCTGGGCACT CACAACCTGG GTCGAGTCCA TTATGAGCCC 
ATTAAAGATC GTCATGGAAA TGTGCTGCTT GTGACGGTGA GGGAAATGGA CAGTCTTTAC 
TCCTTCTTCA ACGGGAAGTG GATGCAAGTC TCCAAACTGC AGAGTCAGAG GAAGTCCCTG 
TCTACACCTG AGGAACCATA TGCCCTCGAC ATCCTACTCA TCACCATACA GGACATTCTG 
GCGTATCAGA GACGGAGTCA GCACCGGCTG TCCTCTGGCC TTTATCTGGG TTACCTGAAA 
CTCAGCAGCT CTGTGGATCA GATCAAGGTT CTGGTTCCTC AGCGGATGCC CAACATGCTT 
TGCCACACCA AGATACGAGA CAACTGGAAC GTGTCAAGGG ATGAGTGGGA ATGGCTGCAG 
TCACTGTCTG GCCCTGTGGA GGTGGAAAGA GCAGATCAGG CTACAGGCTG CCTCCTTTTC 
TCTGAGCTCC AGACAGCCAT TAAAAGTCTG CTGCACCAGA TTAATCTACC TCTCCACCAG 
GCCAAGCACT TCCGTCTCTA CACACACGAG GTGCTGGAGC TGGGTCACAA TGTGTCCTTT 
CTTCTGCTGC TGCCCGCCTC AGACGACGTC TGCTCTGCCC CGGGACAGAC CAACCCCTAC 
ACACCACACT CGGGGTTCCT CAACCTCCCT CTTCAAATGT TTGAGCTCGT TCATTTCTGC 
TCGTATAAGG AGAAATTCAT CAGTCTTTAC TGCCGGCTGT CCTCAGTGCT GGATCTGGAT 
GCTCTGATTA CCCAGCAGGC TTTTAGAGAG GCCATCACTG ACTCAGAGGT GTCCACTGCC 
AAACAGAGGC ACCAGCACAT TCTGGACTAC ATTCAGCAAT TGGACGAGAT GTGGCGAGAA 
GTCCGCTGGA TCACCAATGC GCTGCAGTAC GCCCGCTATA AACAGCCTCT CGGTTGGGTG 
CCGATCACCT GGCTAGTAGA TGTCAGCGTG GAGCCTCCTG TCCAGAAGAA TGACTCCACG 
TCGTCTAACA CGGACTACGT GCCCACTCCC TCACCCTCTC CAGAAATGAG AAGAAGAAAG 
CCCACTATTG AGTCCCAGCC AGGCTCAGAT GAAGAAGGCT GCTCAGAAGT TTTCCTTCCC 
ACTGACAGTG ACTATGACTC CAGTGATGCC CTGAGCCCTC GAGACCTAGA CTTAGTTTAT 
TCCTCAGCCC AGGATCTCTC CCATCAGGCC GTGCATGTCC TCAGTGGCAG CGCTCCCGAT 
GTCCTCCAAA TGCACGATCT TAAATACAGC GTCTGCTCCA AGTCGATCCT GGAGACTGAA 
TCCTGCACAA AAGATTTGGA GGATCTATCG CTCTCCTCTT ACTCCGTGAA GACCACCGAC 
AAACCGTCCC GCTCCAAGTT TCTTGCCGAT GCCCCAACAA AACGCAAGCT GCTGTCCAAA 
AGCCATCCTC AGAGGAGTTA TTTCGGCGGA CCACACCGCT GGCTTCGTGT ACAAAGCGAA 
AGCCACACTC CGTCGCTTTC CGAAGGTATT TACACTAGAC AGAGTGATAT CGACCTCCCG 
CTGGAGACAC CACTTTCAAT ACCGCACTCG CCCACTACCT CCTACAGTCT GGATGAGTAC 
AGGCAGCCCT ACAGAGAATC CAAACCCAAC GTTCGCAGAA TATTTGTAGA GTCATGCAGC 
AAGACTTCAC CCTGTAGAGA TGCACCGCAT TGGGAGGAAG AGGAGGAGAA AGGCTCACGA 
GGAGCAACGG CGTCGGGGGC TCGTCCGGGA TACAGCTCAA CATCGGAAGC TCAGGATGTG 
GATTCAGATG AGCAAACTAA TGAACAAGTG TCGGAGATCC TCAGCAGCAC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-277E21.2 ( XM_009306800.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305075.1 Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49903
Clone ID Related Accession (Same CDS sequence)	XM_021480370.1 , XM_009306800.2
Accession Version	XM_009306800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCGC AGAAGTTTTC ACCACGCCGA AGTGGAAGAC ACGTAACGGG AAGTTTTGAG 
TCCAGTTTCA GGTTTTTACG GGAAGAGGAT GCAGATGTTC TGAGGATCCG CCGCGGTTCC 
ATGAGTCCAG AACCGGAGAA CAAAGAGAAA TGGCTCAAGA TGTCTTATGA GGCCGAGAAA 
ACTTGCCTGT TGGCTCCACA GGAATCACAA ACACCATGTG TCTGCCACAC AGCTAATGAG 
AAATATGAAA TCCCTGGAAT GGGAGACGAC AGTGTTTTAG AGATGCTAAG CTACTCCAAA 
TTCTCGGATC TGGAGACGTG GCTCTGTATG CCATCGACTT TGTTACCCAG AAGCAGGGAT 
TCAGTTTGCT CCCTGCCACC ACCATCCCAT GAGAACGGCA CTGCCGACAC AGACAGAGAA 
TCCAGGCCCC CGGAGAACAC GTCTATCAAT CTGAGCCAGT GCCAGGAGAG GAAAACCCAT 
CTATTGCCAC CCTCATCGCT CATGCCAGTC TCAGCCTCCC CTACATCCTC AACTCCCCTC 
CGGACCACCT CAACTCCTCT GCCTAAACCC AACAGAGACT CCGGTCAGGG CGGTGTGAGT 
GTTCGCAAAC GCCGGCGTCT GGCTGCTAGC CCTGGAGGAC TGCACTGGGA TGCATCAGGA 
TCAGTGCTGC GTGACCATGA CCGAGGGGAG ACGCCGTCAC TGTCGGAAGT GAAGCGCTTC 
CCTAGAAGTG TGGATTCGGA GAGCCTCGTT CCAGAACTCT GGAGGGACCG AGCACCTCTT 
AGGAAGACCA TTTCTATCGA TGACCGCCTT CTCCAGCAAA CGCCCAGAGA ACATCACAGA 
CTCCTCAGCC GACTGGAGAG AGGCAAGAAG AAACTCAGGA ACATAAATAG TCTTGGAGCC 
ACTGGCAGAT ACGAAACCCA TAAAAAATCC GAGAGCAGGA TCTCCCGTCT GGCTCAGAGA 
CTCAACCAGA GGCAGAGCGA TGCGGCATTG ATTAAGGATT TCAGACCTCT TTTCCTCCTC 
TCTGGATCTG CTGGAAGCTC CCAGAGTCTG GACAGGAACT TCTCCATCAG TATGAGCCAG 
CAGATGCAGA ACCTGCAGCT GACGCAGAGC AAGAAGGGGG CTGGTCCCGC CTCCCCCAGC 
GCTGCCAAAC GCCTCTACCG GAACCTCTCC GAGAAGTTTA AAGGGAGCCA CTCATCCTTC 
GAGGATGCCT ACTTTTTCGG CCGATCCGAC CGTATACGCA AAGTATCAAA CATCCAGAGC 
AGCGAGGCTC TGTTCGAGGC TGTTGAACAG CAGGATCTGG ATGCCGTTCA GATCCTGCTG 
TTTCAGTACA CAGCTGATGA ACTGGACCTG AACACCCCCA ATAGTGAAGG CCTGACGCCT 
CTGGACATCT CCATCATGAC CAATAACGTT CCCATCGCCA AACTGTTGCT TAAAGCTGGA 
GGGAAGGAGA GCCCTCACTT TGTCAGTTTG GAGAGCAGAG ACGCTCACCT CTCAGCACTA 
GTCCAGGAAG CCCAAAGGAG AGCCAGTGAA CTGTCCAACC AGGTGATGAG AGAAAGTCTC 
AGCCTGGAAA CGTCTGACAA AGAAAAACAG CTCAAAGCTT GGGAGTGGCG ATGCAAACTC 
TACAAGCGCA TGCGCACAGG ATTCGAGCAT GCACGGCAGC CGGAGGCTCC ATTGATGGTG 
CGTCTGAGTG TGACCGGCAG CACGACTCTC ACTGTTAGCT TTCAGGAACC TGCCAGCATG 
AATTCAGCTG TGGTGACCAA ATACAAAGTT GAATGGAGCT GTCTGAAGGA CTTCTCTTTG 
TTAGCCGGAG AACTGATCCT TGAGAATCTG CAGTCCCTCA AATGCACAAT TACAGGCCTC 
ACCATGGGTA GGCAGTACTA TGTTCAAGTC TCTGCTTACA ATATGAAGGG TTGGGGTCCA 
GCACAACTTT CTCAGCCTCC TTCAGCTGTT CCCTCCAACT GGAAGGACTG TGATGGGAGA 
GAAAGCAGGA GGAGAGGTCA CATCGAGGCC ATGGAGAGGC TCTTACAGCA GGTTCGAGCC 
ACACACCAGC ACTACTGCTG TGGAGACACA TCAAAGTTGC AGAATCCAAG TCGTAAACAG 
TCTGTGTCCC GGAGCCTGAA GCACCTTTTT CACTCCTCTA CGAAATTTGT CAAGAGTCTT 
AAAAGGGGAG TTTATATAGC GTCAGTATTT TACCATAAGG ACAGTCTGCT AGTGACTAAT 
GAGGATCAGA TCCCCATAGT GGAGGTGGAC GATTCATACA GCAGCTCCCT CATGCAGGAC 
TTTCTGTGGT TCACCAAGCT TTCTTGTATG TGGGAGGACG TCCGGTGGCT CAGACAAAGC 
CTGGCAGTCT CCACCTCATC CTCTTCCACT CTGCAGTCCA GACAGAAGAT GCTGGCGGCC 
GCGGGACAAC TGCAGAATCT TCTGGGCACT CACAACCTGG GTCGAGTCCA TTATGAGCCC 
ATTAAAGATC GTCATGGAAA TGTGCTGCTT GTGACGGTGA GGGAAATGGA CAGTCTTTAC 
TCCTTCTTCA ACGGGAAGTG GATGCAAGTC TCCAAACTGC AGAGTCAGAG GAAGTCCCTG 
TCTACACCTG AGGAACCATA TGCCCTCGAC ATCCTACTCA TCACCATACA GGACATTCTG 
GCGTATCAGA GACGGAGTCA GCACCGGCTG TCCTCTGGCC TTTATCTGGG TTACCTGAAA 
CTCAGCAGCT CTGTGGATCA GATCAAGGTT CTGGTTCCTC AGCGGATGCC CAACATGCTT 
TGCCACACCA AGATACGAGA CAACTGGAAC GTGTCAAGGG ATGAGTGGGA ATGGCTGCAG 
TCACTGTCTG GCCCTGTGGA GGTGGAAAGA GCAGATCAGG CTACAGGCTG CCTCCTTTTC 
TCTGAGCTCC AGACAGCCAT TAAAAGTCTG CTGCACCAGA TTAATCTACC TCTCCACCAG 
GCCAAGCACT TCCGTCTCTA CACACACGAG GTGCTGGAGC TGGGTCACAA TGTGTCCTTT 
CTTCTGCTGC TGCCCGCCTC AGACGACGTC TGCTCTGCCC CGGGACAGAC CAACCCCTAC 
ACACCACACT CGGGGTTCCT CAACCTCCCT CTTCAAATGT TTGAGCTCGT TCATTTCTGC 
TCGTATAAGG AGAAATTCAT CAGTCTTTAC TGCCGGCTGT CCTCAGTGCT GGATCTGGAT 
GCTCTGATTA CCCAGCAGGC TTTTAGAGAG GCCATCACTG ACTCAGAGGT GTCCACTGCC 
AAACAGAGGC ACCAGCACAT TCTGGACTAC ATTCAGCAAT TGGACGAGAT GTGGCGAGAA 
GTCCGCTGGA TCACCAATGC GCTGCAGTAC GCCCGCTATA AACAGCCTCT CGGTTGGGTG 
CCGATCACCT GGCTAGTAGA TGTCAGCGTG GAGCCTCCTG TCCAGAAGAA TGACTCCACG 
TCGTCTAACA CGGACTACGT GCCCACTCCC TCACCCTCTC CAGAAATGAG AAGAAGAAAG 
CCCACTATTG AGTCCCAGCC AGGCTCAGAT GAAGAAGGCT GCTCAGAAGT TTTCCTTCCC 
ACTGACAGTG ACTATGACTC CAGTGATGCC CTGAGCCCTC GAGACCTAGA CTTAGTTTAT 
TCCTCAGCCC AGGATCTCTC CCATCAGGCC GTGCATGTCC TCAGTGGCAG CGCTCCCGAT 
GTCCTCCAAA TGCACGATCT TAAATACAGC GTCTGCTCCA AGTCGATCCT GGAGACTGAA 
TCCTGCACAA AAGATTTGGA GGATCTATCG CTCTCCTCTT ACTCCGTGAA GACCACCGAC 
AAACCGTCCC GCTCCAAGTT TCTTGCCGAT GCCCCAACAA AACGCAAGCT GCTGTCCAAA 
AGCCATCCTC AGAGGAGTTA TTTCGGCGGA CCACACCGCT GGCTTCGTGT ACAAAGCGAA 
AGCCACACTC CGTCGCTTTC CGAAGGTATT TACACTAGAC AGAGTGATAT CGACCTCCCG 
CTGGAGACAC CACTTTCAAT ACCGCACTCG CCCACTACCT CCTACAGTCT GGATGAGTAC 
AGGCAGCCCT ACAGAGAATC CAAACCCAAC GTTCGCAGAA TATTTGTAGA GTCATGCAGC 
AAGACTTCAC CCTGTAGAGA TGCACCGCAT TGGGAGGAAG AGGAGGAGAA AGGCTCACGA 
GGAGCAACGG CGTCGGGGGC TCGTCCGGGA TACAGCTCAA CATCGGAAGC TCAGGATGTG 
GATTCAGATG AGCAAACTAA TGAACAAGTG TCGGAGATCC TCAGCAGCAC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305075.1
CDS	592..4848
Translation	MLSQKFSPRRSGRHVTGSFESSFRFLREEDADVLRIRRGSMSPEPENKEKWLKMSYEAEKTCLLAPQESQTPCVCHTANEKYEIPGMGDDSVLEMLSYSKFSDLETWLCMPSTLLPRSRDSVCSLPPPSHENGTADTDRESRPPENTSINLSQCQERKTHLLPPSSLMPVSASPTSSTPLRTTSTPLPKPNRDSGQGGVSVRKRRRLAASPGGLHWDASGSVLRDHDRGETPSLSEVKRFPRSVDSESLVPELWRDRAPLRKTISIDDRLLQQTPREHHRLLSRLERGKKKLRNINSLGATGRYETHKKSESRISRLAQRLNQRQSDAALIKDFRPLFLLSGSAGSSQSLDRNFSISMSQQMQNLQLTQSKKGAGPASPSAAKRLYRNLSEKFKGSHSSFEDAYFFGRSDRIRKVSNIQSSEALFEAVEQQDLDAVQILLFQYTADELDLNTPNSEGLTPLDISIMTNNVPIAKLLLKAGGKESPHFVSLESRDAHLSALVQEAQRRASELSNQVMRESLSLETSDKEKQLKAWEWRCKLYKRMRTGFEHARQPEAPLMVRLSVTGSTTLTVSFQEPASMNSAVVTKYKVEWSCLKDFSLLAGELILENLQSLKCTITGLTMGRQYYVQVSAYNMKGWGPAQLSQPPSAVPSNWKDCDGRESRRRGHIEAMERLLQQVRATHQHYCCGDTSKLQNPSRKQSVSRSLKHLFHSSTKFVKSLKRGVYIASVFYHKDSLLVTNEDQIPIVEVDDSYSSSLMQDFLWFTKLSCMWEDVRWLRQSLAVSTSSSSTLQSRQKMLAAAGQLQNLLGTHNLGRVHYEPIKDRHGNVLLVTVREMDSLYSFFNGKWMQVSKLQSQRKSLSTPEEPYALDILLITIQDILAYQRRSQHRLSSGLYLGYLKLSSSVDQIKVLVPQRMPNMLCHTKIRDNWNVSRDEWEWLQSLSGPVEVERADQATGCLLFSELQTAIKSLLHQINLPLHQAKHFRLYTHEVLELGHNVSFLLLLPASDDVCSAPGQTNPYTPHSGFLNLPLQMFELVHFCSYKEKFISLYCRLSSVLDLDALITQQAFREAITDSEVSTAKQRHQHILDYIQQLDEMWREVRWITNALQYARYKQPLGWVPITWLVDVSVEPPVQKNDSTSSNTDYVPTPSPSPEMRRRKPTIESQPGSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYSSAQDLSHQAVHVLSGSAPDVLQMHDLKYSVCSKSILETESCTKDLEDLSLSSYSVKTTDKPSRSKFLADAPTKRKLLSKSHPQRSYFGGPHRWLRVQSESHTPSLSEGIYTRQSDIDLPLETPLSIPHSPTTSYSLDEYRQPYRESKPNVRRIFVESCSKTSPCRDAPHWEEEEEKGSRGATASGARPGYSSTSEAQDVDSDEQTNEQVSEILSSTL

Target ORF information:

RefSeq Version	XM_009306800.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306800.2

ORF Insert Sequence:

ATGCTGTCGC AGAAGTTTTC ACCACGCCGA AGTGGAAGAC ACGTAACGGG AAGTTTTGAG 
TCCAGTTTCA GGTTTTTACG GGAAGAGGAT GCAGATGTTC TGAGGATCCG CCGCGGTTCC 
ATGAGTCCAG AACCGGAGAA CAAAGAGAAA TGGCTCAAGA TGTCTTATGA GGCCGAGAAA 
ACTTGCCTGT TGGCTCCACA GGAATCACAA ACACCATGTG TCTGCCACAC AGCTAATGAG 
AAATATGAAA TCCCTGGAAT GGGAGACGAC AGTGTTTTAG AGATGCTAAG CTACTCCAAA 
TTCTCGGATC TGGAGACGTG GCTCTGTATG CCATCGACTT TGTTACCCAG AAGCAGGGAT 
TCAGTTTGCT CCCTGCCACC ACCATCCCAT GAGAACGGCA CTGCCGACAC AGACAGAGAA 
TCCAGGCCCC CGGAGAACAC GTCTATCAAT CTGAGCCAGT GCCAGGAGAG GAAAACCCAT 
CTATTGCCAC CCTCATCGCT CATGCCAGTC TCAGCCTCCC CTACATCCTC AACTCCCCTC 
CGGACCACCT CAACTCCTCT GCCTAAACCC AACAGAGACT CCGGTCAGGG CGGTGTGAGT 
GTTCGCAAAC GCCGGCGTCT GGCTGCTAGC CCTGGAGGAC TGCACTGGGA TGCATCAGGA 
TCAGTGCTGC GTGACCATGA CCGAGGGGAG ACGCCGTCAC TGTCGGAAGT GAAGCGCTTC 
CCTAGAAGTG TGGATTCGGA GAGCCTCGTT CCAGAACTCT GGAGGGACCG AGCACCTCTT 
AGGAAGACCA TTTCTATCGA TGACCGCCTT CTCCAGCAAA CGCCCAGAGA ACATCACAGA 
CTCCTCAGCC GACTGGAGAG AGGCAAGAAG AAACTCAGGA ACATAAATAG TCTTGGAGCC 
ACTGGCAGAT ACGAAACCCA TAAAAAATCC GAGAGCAGGA TCTCCCGTCT GGCTCAGAGA 
CTCAACCAGA GGCAGAGCGA TGCGGCATTG ATTAAGGATT TCAGACCTCT TTTCCTCCTC 
TCTGGATCTG CTGGAAGCTC CCAGAGTCTG GACAGGAACT TCTCCATCAG TATGAGCCAG 
CAGATGCAGA ACCTGCAGCT GACGCAGAGC AAGAAGGGGG CTGGTCCCGC CTCCCCCAGC 
GCTGCCAAAC GCCTCTACCG GAACCTCTCC GAGAAGTTTA AAGGGAGCCA CTCATCCTTC 
GAGGATGCCT ACTTTTTCGG CCGATCCGAC CGTATACGCA AAGTATCAAA CATCCAGAGC 
AGCGAGGCTC TGTTCGAGGC TGTTGAACAG CAGGATCTGG ATGCCGTTCA GATCCTGCTG 
TTTCAGTACA CAGCTGATGA ACTGGACCTG AACACCCCCA ATAGTGAAGG CCTGACGCCT 
CTGGACATCT CCATCATGAC CAATAACGTT CCCATCGCCA AACTGTTGCT TAAAGCTGGA 
GGGAAGGAGA GCCCTCACTT TGTCAGTTTG GAGAGCAGAG ACGCTCACCT CTCAGCACTA 
GTCCAGGAAG CCCAAAGGAG AGCCAGTGAA CTGTCCAACC AGGTGATGAG AGAAAGTCTC 
AGCCTGGAAA CGTCTGACAA AGAAAAACAG CTCAAAGCTT GGGAGTGGCG ATGCAAACTC 
TACAAGCGCA TGCGCACAGG ATTCGAGCAT GCACGGCAGC CGGAGGCTCC ATTGATGGTG 
CGTCTGAGTG TGACCGGCAG CACGACTCTC ACTGTTAGCT TTCAGGAACC TGCCAGCATG 
AATTCAGCTG TGGTGACCAA ATACAAAGTT GAATGGAGCT GTCTGAAGGA CTTCTCTTTG 
TTAGCCGGAG AACTGATCCT TGAGAATCTG CAGTCCCTCA AATGCACAAT TACAGGCCTC 
ACCATGGGTA GGCAGTACTA TGTTCAAGTC TCTGCTTACA ATATGAAGGG TTGGGGTCCA 
GCACAACTTT CTCAGCCTCC TTCAGCTGTT CCCTCCAACT GGAAGGACTG TGATGGGAGA 
GAAAGCAGGA GGAGAGGTCA CATCGAGGCC ATGGAGAGGC TCTTACAGCA GGTTCGAGCC 
ACACACCAGC ACTACTGCTG TGGAGACACA TCAAAGTTGC AGAATCCAAG TCGTAAACAG 
TCTGTGTCCC GGAGCCTGAA GCACCTTTTT CACTCCTCTA CGAAATTTGT CAAGAGTCTT 
AAAAGGGGAG TTTATATAGC GTCAGTATTT TACCATAAGG ACAGTCTGCT AGTGACTAAT 
GAGGATCAGA TCCCCATAGT GGAGGTGGAC GATTCATACA GCAGCTCCCT CATGCAGGAC 
TTTCTGTGGT TCACCAAGCT TTCTTGTATG TGGGAGGACG TCCGGTGGCT CAGACAAAGC 
CTGGCAGTCT CCACCTCATC CTCTTCCACT CTGCAGTCCA GACAGAAGAT GCTGGCGGCC 
GCGGGACAAC TGCAGAATCT TCTGGGCACT CACAACCTGG GTCGAGTCCA TTATGAGCCC 
ATTAAAGATC GTCATGGAAA TGTGCTGCTT GTGACGGTGA GGGAAATGGA CAGTCTTTAC 
TCCTTCTTCA ACGGGAAGTG GATGCAAGTC TCCAAACTGC AGAGTCAGAG GAAGTCCCTG 
TCTACACCTG AGGAACCATA TGCCCTCGAC ATCCTACTCA TCACCATACA GGACATTCTG 
GCGTATCAGA GACGGAGTCA GCACCGGCTG TCCTCTGGCC TTTATCTGGG TTACCTGAAA 
CTCAGCAGCT CTGTGGATCA GATCAAGGTT CTGGTTCCTC AGCGGATGCC CAACATGCTT 
TGCCACACCA AGATACGAGA CAACTGGAAC GTGTCAAGGG ATGAGTGGGA ATGGCTGCAG 
TCACTGTCTG GCCCTGTGGA GGTGGAAAGA GCAGATCAGG CTACAGGCTG CCTCCTTTTC 
TCTGAGCTCC AGACAGCCAT TAAAAGTCTG CTGCACCAGA TTAATCTACC TCTCCACCAG 
GCCAAGCACT TCCGTCTCTA CACACACGAG GTGCTGGAGC TGGGTCACAA TGTGTCCTTT 
CTTCTGCTGC TGCCCGCCTC AGACGACGTC TGCTCTGCCC CGGGACAGAC CAACCCCTAC 
ACACCACACT CGGGGTTCCT CAACCTCCCT CTTCAAATGT TTGAGCTCGT TCATTTCTGC 
TCGTATAAGG AGAAATTCAT CAGTCTTTAC TGCCGGCTGT CCTCAGTGCT GGATCTGGAT 
GCTCTGATTA CCCAGCAGGC TTTTAGAGAG GCCATCACTG ACTCAGAGGT GTCCACTGCC 
AAACAGAGGC ACCAGCACAT TCTGGACTAC ATTCAGCAAT TGGACGAGAT GTGGCGAGAA 
GTCCGCTGGA TCACCAATGC GCTGCAGTAC GCCCGCTATA AACAGCCTCT CGGTTGGGTG 
CCGATCACCT GGCTAGTAGA TGTCAGCGTG GAGCCTCCTG TCCAGAAGAA TGACTCCACG 
TCGTCTAACA CGGACTACGT GCCCACTCCC TCACCCTCTC CAGAAATGAG AAGAAGAAAG 
CCCACTATTG AGTCCCAGCC AGGCTCAGAT GAAGAAGGCT GCTCAGAAGT TTTCCTTCCC 
ACTGACAGTG ACTATGACTC CAGTGATGCC CTGAGCCCTC GAGACCTAGA CTTAGTTTAT 
TCCTCAGCCC AGGATCTCTC CCATCAGGCC GTGCATGTCC TCAGTGGCAG CGCTCCCGAT 
GTCCTCCAAA TGCACGATCT TAAATACAGC GTCTGCTCCA AGTCGATCCT GGAGACTGAA 
TCCTGCACAA AAGATTTGGA GGATCTATCG CTCTCCTCTT ACTCCGTGAA GACCACCGAC 
AAACCGTCCC GCTCCAAGTT TCTTGCCGAT GCCCCAACAA AACGCAAGCT GCTGTCCAAA 
AGCCATCCTC AGAGGAGTTA TTTCGGCGGA CCACACCGCT GGCTTCGTGT ACAAAGCGAA 
AGCCACACTC CGTCGCTTTC CGAAGGTATT TACACTAGAC AGAGTGATAT CGACCTCCCG 
CTGGAGACAC CACTTTCAAT ACCGCACTCG CCCACTACCT CCTACAGTCT GGATGAGTAC 
AGGCAGCCCT ACAGAGAATC CAAACCCAAC GTTCGCAGAA TATTTGTAGA GTCATGCAGC 
AAGACTTCAC CCTGTAGAGA TGCACCGCAT TGGGAGGAAG AGGAGGAGAA AGGCTCACGA 
GGAGCAACGG CGTCGGGGGC TCGTCCGGGA TACAGCTCAA CATCGGAAGC TCAGGATGTG 
GATTCAGATG AGCAAACTAA TGAACAAGTG TCGGAGATCC TCAGCAGCAC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-277E21.2 ( XM_009306801.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305076.1 Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49904
Clone ID Related Accession (Same CDS sequence)	XM_009306801.2
Accession Version	XM_009306801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGGT TCATTTACTT CCTGATGGCG AACAGCGATC ACATTTGTTT CAGGTTTTTA 
CGGGAAGAGG ATGCAGATGT TCTGAGGATC CGCCGCGGTT CCATGAGTCC AGAACCGGAG 
AACAAAGAGA AATGGCTCAA GATGTCTTAT GAGGCCGAGA AAACTTGCCT GTTGGCTCCA 
CAGGAATCAC AAACACCATG TGTCTGCCAC ACAGCTAATG AGAAATATGA AATCCCTGGA 
ATGGGAGACG ACAGTGTTTT AGAGATGCTA AGCTACTCCA AATTCTCGGA TCTGGAGACG 
TGGCTCTGTA TGCCATCGAC TTTGTTACCC AGAAGCAGGG ATTCAGTTTG CTCCCTGCCA 
CCACCATCCC ATGAGAACGG CACTGCCGAC ACAGACAGAG AATCCAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTGGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGACGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG CATGCGCACA 
GGATTCGAGC ATGCACGGCA GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGGC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG AAGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACGGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GACTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTG 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGTGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAGGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC GGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCGGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305076.1
CDS	508..4746
Translation	MTGFIYFLMANSDHICFRFLREEDADVLRIRRGSMSPEPENKEKWLKMSYEAEKTCLLAPQESQTPCVCHTANEKYEIPGMGDDSVLEMLSYSKFSDLETWLCMPSTLLPRSRDSVCSLPPPSHENGTADTDRESRPPENTSINLSQCQERKTHLLPPSSLMPVSASPTSSTPLRTTSTPLPKPNRDSGQGGVSVRKRRRLAASPGGLHWDASGSVLRDHDRGETPSLSEVKRFPRSVDSESLVPELWRDRAPLRKTISIDDRLLQQTPREHHRLLSRLERGKKKLRNINSLGATGRYETHKKSESRISRLAQRLNQRQSDAALIKDFRPLFLLSGSAGSSQSLDRNFSISMSQQMQNLQLTQSKKGAGPASPSAAKRLYRNLSEKFKGSHSSFEDAYFFGRSDRIRKVSNIQSSEALFEAVEQQDLDAVQILLFQYTADELDLNTPNSEGLTPLDISIMTNNVPIAKLLLKAGGKESPHFVSLESRDAHLSALVQEAQRRASELSNQVMRESLSLETSDKEKQLKAWEWRCKLYKRMRTGFEHARQPEAPLMVRLSVTGSTTLTVSFQEPASMNSAVVTKYKVEWSCLKDFSLLAGELILENLQSLKCTITGLTMGRQYYVQVSAYNMKGWGPAQLSQPPSAVPSNWKDCDGRESRRRGHIEAMERLLQQVRATHQHYCCGDTSKLQNPSRKQSVSRSLKHLFHSSTKFVKSLKRGVYIASVFYHKDSLLVTNEDQIPIVEVDDSYSSSLMQDFLWFTKLSCMWEDVRWLRQSLAVSTSSSSTLQSRQKMLAAAGQLQNLLGTHNLGRVHYEPIKDRHGNVLLVTVREMDSLYSFFNGKWMQVSKLQSQRKSLSTPEEPYALDILLITIQDILAYQRRSQHRLSSGLYLGYLKLSSSVDQIKVLVPQRMPNMLCHTKIRDNWNVSRDEWEWLQSLSGPVEVERADQATGCLLFSELQTAIKSLLHQINLPLHQAKHFRLYTHEVLELGHNVSFLLLLPASDDVCSAPGQTNPYTPHSGFLNLPLQMFELVHFCSYKEKFISLYCRLSSVLDLDALITQQAFREAITDSEVSTAKQRHQHILDYIQQLDEMWREVRWITNALQYARYKQPLGWVPITWLVDVSVEPPVQKNDSTSSNTDYVPTPSPSPEMRRRKPTIESQPGSDEEGCSEVFLPTDSDYDSSDALSPRDLDLVYSSAQDLSHQAVHVLSGSAPDVLQMHDLKYSVCSKSILETESCTKDLEDLSLSSYSVKTTDKPSRSKFLADAPTKRKLLSKSHPQRSYFGGPHRWLRVQSESHTPSLSEGIYTRQSDIDLPLETPLSIPHSPTTSYSLDEYRQPYRESKPNVRRIFVESCSKTSPCRDAPHWEEEEEKGSRGATASGARPGYSSTSEAQDVDSDEQTNEQVSEILSSTL

Target ORF information:

RefSeq Version	XM_009306801.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-277e21.2 (si:ch211-277e21.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306801.2

ORF Insert Sequence:

ATGACAGGGT TCATTTACTT CCTGATGGCG AACAGCGATC ACATTTGTTT CAGGTTTTTA 
CGGGAAGAGG ATGCAGATGT TCTGAGGATC CGCCGCGGTT CCATGAGTCC AGAACCGGAG 
AACAAAGAGA AATGGCTCAA GATGTCTTAT GAGGCCGAGA AAACTTGCCT GTTGGCTCCA 
CAGGAATCAC AAACACCATG TGTCTGCCAC ACAGCTAATG AGAAATATGA AATCCCTGGA 
ATGGGAGACG ACAGTGTTTT AGAGATGCTA AGCTACTCCA AATTCTCGGA TCTGGAGACG 
TGGCTCTGTA TGCCATCGAC TTTGTTACCC AGAAGCAGGG ATTCAGTTTG CTCCCTGCCA 
CCACCATCCC ATGAGAACGG CACTGCCGAC ACAGACAGAG AATCCAGGCC CCCGGAGAAC 
ACGTCTATCA ATCTGAGCCA GTGCCAGGAG AGGAAAACCC ATCTATTGCC ACCCTCATCG 
CTCATGCCAG TCTCAGCCTC CCCTACATCC TCAACTCCCC TCCGGACCAC CTCAACTCCT 
CTGCCTAAAC CCAACAGAGA CTCCGGTCAG GGCGGTGTGA GTGTTCGCAA ACGCCGGCGT 
CTGGCTGCTA GCCCTGGAGG ACTGCACTGG GATGCATCAG GATCAGTGCT GCGTGACCAT 
GACCGAGGGG AGACGCCGTC ACTGTCGGAA GTGAAGCGCT TCCCTAGAAG TGTGGATTCG 
GAGAGCCTCG TTCCAGAACT CTGGAGGGAC CGAGCACCTC TTAGGAAGAC CATTTCTATC 
GATGACCGCC TTCTCCAGCA AACGCCCAGA GAACATCACA GACTCCTCAG CCGACTGGAG 
AGAGGCAAGA AGAAACTCAG GAACATAAAT AGTCTTGGAG CCACTGGCAG ATACGAAACC 
CATAAAAAAT CCGAGAGCAG GATCTCCCGT CTGGCTCAGA GACTCAACCA GAGGCAGAGC 
GATGCGGCAT TGATTAAGGA TTTCAGACCT CTTTTCCTCC TCTCTGGATC TGCTGGAAGC 
TCCCAGAGTC TGGACAGGAA CTTCTCCATC AGTATGAGCC AGCAGATGCA GAACCTGCAG 
CTGACGCAGA GCAAGAAGGG GGCTGGTCCC GCCTCCCCCA GCGCTGCCAA ACGCCTCTAC 
CGGAACCTCT CCGAGAAGTT TAAAGGGAGC CACTCATCCT TCGAGGATGC CTACTTTTTC 
GGCCGATCCG ACCGTATACG CAAAGTATCA AACATCCAGA GCAGCGAGGC TCTGTTCGAG 
GCTGTTGAAC AGCAGGATCT GGATGCCGTT CAGATCCTGC TGTTTCAGTA CACAGCTGAT 
GAACTGGACC TGAACACCCC CAATAGTGAA GGCCTGACGC CTCTGGACAT CTCCATCATG 
ACCAATAACG TTCCCATCGC CAAACTGTTG CTTAAAGCTG GAGGGAAGGA GAGCCCTCAC 
TTTGTCAGTT TGGAGAGCAG AGACGCTCAC CTCTCAGCAC TAGTCCAGGA AGCCCAAAGG 
AGAGCCAGTG AACTGTCCAA CCAGGTGATG AGAGAAAGTC TCAGCCTGGA AACGTCTGAC 
AAAGAAAAAC AGCTCAAAGC TTGGGAGTGG CGATGCAAAC TCTACAAGCG CATGCGCACA 
GGATTCGAGC ATGCACGGCA GCCGGAGGCT CCATTGATGG TGCGTCTGAG TGTGACCGGC 
AGCACGACTC TCACTGTTAG CTTTCAGGAA CCTGCCAGCA TGAATTCAGC TGTGGTGACC 
AAATACAAAG TTGAATGGAG CTGTCTGAAG GACTTCTCTT TGTTAGCCGG AGAACTGATC 
CTTGAGAATC TGCAGTCCCT CAAATGCACA ATTACAGGCC TCACCATGGG TAGGCAGTAC 
TATGTTCAAG TCTCTGCTTA CAATATGAAG GGTTGGGGTC CAGCACAACT TTCTCAGCCT 
CCTTCAGCTG TTCCCTCCAA CTGGAAGGAC TGTGATGGGA GAGAAAGCAG GAGGAGAGGT 
CACATCGAGG CCATGGAGAG GCTCTTACAG CAGGTTCGAG CCACACACCA GCACTACTGC 
TGTGGAGACA CATCAAAGTT GCAGAATCCA AGTCGTAAAC AGTCTGTGTC CCGGAGCCTG 
AAGCACCTTT TTCACTCCTC TACGAAATTT GTCAAGAGTC TTAAAAGGGG AGTTTATATA 
GCGTCAGTAT TTTACCATAA GGACAGTCTG CTAGTGACTA ATGAGGATCA GATCCCCATA 
GTGGAGGTGG ACGATTCATA CAGCAGCTCC CTCATGCAGG ACTTTCTGTG GTTCACCAAG 
CTTTCTTGTA TGTGGGAGGA CGTCCGGTGG CTCAGACAAA GCCTGGCAGT CTCCACCTCA 
TCCTCTTCCA CTCTGCAGTC CAGACAGAAG ATGCTGGCGG CCGCGGGACA ACTGCAGAAT 
CTTCTGGGCA CTCACAACCT GGGTCGAGTC CATTATGAGC CCATTAAAGA TCGTCATGGA 
AATGTGCTGC TTGTGACGGT GAGGGAAATG GACAGTCTTT ACTCCTTCTT CAACGGGAAG 
TGGATGCAAG TCTCCAAACT GCAGAGTCAG AGGAAGTCCC TGTCTACACC TGAGGAACCA 
TATGCCCTCG ACATCCTACT CATCACCATA CAGGACATTC TGGCGTATCA GAGACGGAGT 
CAGCACCGGC TGTCCTCTGG CCTTTATCTG GGTTACCTGA AACTCAGCAG CTCTGTGGAT 
CAGATCAAGG TTCTGGTTCC TCAGCGGATG CCCAACATGC TTTGCCACAC CAAGATACGA 
GACAACTGGA ACGTGTCAAG GGATGAGTGG GAATGGCTGC AGTCACTGTC TGGCCCTGTG 
GAGGTGGAAA GAGCAGATCA GGCTACAGGC TGCCTCCTTT TCTCTGAGCT CCAGACAGCC 
ATTAAAAGTC TGCTGCACCA GATTAATCTA CCTCTCCACC AGGCCAAGCA CTTCCGTCTC 
TACACACACG AGGTGCTGGA GCTGGGTCAC AATGTGTCCT TTCTTCTGCT GCTGCCCGCC 
TCAGACGACG TCTGCTCTGC CCCGGGACAG ACCAACCCCT ACACACCACA CTCGGGGTTC 
CTCAACCTCC CTCTTCAAAT GTTTGAGCTC GTTCATTTCT GCTCGTATAA GGAGAAATTC 
ATCAGTCTTT ACTGCCGGCT GTCCTCAGTG CTGGATCTGG ATGCTCTGAT TACCCAGCAG 
GCTTTTAGAG AGGCCATCAC TGACTCAGAG GTGTCCACTG CCAAACAGAG GCACCAGCAC 
ATTCTGGACT ACATTCAGCA ATTGGACGAG ATGTGGCGAG AAGTCCGCTG GATCACCAAT 
GCGCTGCAGT ACGCCCGCTA TAAACAGCCT CTCGGTTGGG TGCCGATCAC CTGGCTAGTA 
GATGTCAGCG TGGAGCCTCC TGTCCAGAAG AATGACTCCA CGTCGTCTAA CACGGACTAC 
GTGCCCACTC CCTCACCCTC TCCAGAAATG AGAAGAAGAA AGCCCACTAT TGAGTCCCAG 
CCAGGCTCAG ATGAAGAAGG CTGCTCAGAA GTTTTCCTTC CCACTGACAG TGACTATGAC 
TCCAGTGATG CCCTGAGCCC TCGAGACCTA GACTTAGTTT ATTCCTCAGC CCAGGATCTC 
TCCCATCAGG CCGTGCATGT CCTCAGTGGC AGCGCTCCCG ATGTCCTCCA AATGCACGAT 
CTTAAATACA GCGTCTGCTC CAAGTCGATC CTGGAGACTG AATCCTGCAC AAAAGATTTG 
GAGGATCTAT CGCTCTCCTC TTACTCCGTG AAGACCACCG ACAAACCGTC CCGCTCCAAG 
TTTCTTGCCG ATGCCCCAAC AAAACGCAAG CTGCTGTCCA AAAGCCATCC TCAGAGGAGT 
TATTTCGGCG GACCACACCG CTGGCTTCGT GTACAAAGCG AAAGCCACAC TCCGTCGCTT 
TCCGAAGGTA TTTACACTAG ACAGAGTGAT ATCGACCTCC CGCTGGAGAC ACCACTTTCA 
ATACCGCACT CGCCCACTAC CTCCTACAGT CTGGATGAGT ACAGGCAGCC CTACAGAGAA 
TCCAAACCCA ACGTTCGCAG AATATTTGTA GAGTCATGCA GCAAGACTTC ACCCTGTAGA 
GATGCACCGC ATTGGGAGGA AGAGGAGGAG AAAGGCTCAC GAGGAGCAAC GGCGTCGGGG 
GCTCGTCCGG GATACAGCTC AACATCGGAA GCTCAGGATG TGGATTCAGA TGAGCAAACT 
AATGAACAAG TGTCGGAGAT CCTCAGCAGC ACTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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SI:CH211-277E21.2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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