H3f4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H3f4
Entrez Gene ID	382523
Full Name	predicted gene 12260
Synonyms	H3t
General protein information	Preferred Names predicted gene 12260 Names predicted gene 12260 histone cluster 1, H3 pseudogene
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001318003.2	NP_001304932.1	predicted gene 12260

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis.
Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H, Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T, Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H, Ohkawa Y, Kurumizaka H, Yamagata K
Cell reports18(3)593-600(2017 01)

Tissue-specific expression of histone H3 variants diversified after species separation.
Maehara K, Harada A, Sato Y, Matsumoto M, Nakayama KI, Kimura H, Ohkawa Y
Epigenetics & chromatin835(2015)

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
Govin J, Escoffier E, Rousseaux S, Kuhn L, Ferro M, Th?venon J, Catena R, Davidson I, Garin J, Khochbin S, Caron C
The Journal of cell biology176(3)283-94(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following H3f4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H3f4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83441	NM_001318003.2 Latest version!	Mus musculus H3.4 histone (H3f4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H3f4 ( NM_001318003.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001304932.1 Mus musculus H3.4 histone (H3f4), mRNA.

Price & Availability↑

CloneID	OMu83441
Clone ID Related Accession (Same CDS sequence)	NM_001318003.2
Accession Version	NM_001318003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Mus musculus(house mouse)
Product	predicted gene 12260
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL662809.14. On Dec 19, 2015 this sequence version replaced NM_001318003.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 26388943 ##RefSeq-Attributes-END##

ATGGCACGCA CCAAGCAGAC GGCACGGAAG TCGACGGGAG GCAAGGCTCC GCGCAAGCAG 
CTAGCCACGA AGGTGGCCCG CAAGAGCGCC CCGGCCACCG GGGGCGTGAA GAAGCCGCAC 
CGCTACCACC CTGGCACGGT GGCGCTGCGC GAGATCCGGC GCTACCAGAA GTCCACCGAG 
CTGCTGATTC GCAAGTTGCC ATTCCAGCGC TTGGTGCGTG AGATCGCCCA GGACTTCAAG 
ACCGACCTGC GCTTCCAGAG CTCCGCGGTG ATGGCGCTGC AGGAGGCCTG CGAGTCGTAC 
CTCGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCTATCC ACGCCAAGCG GGTGACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGGCGC ATCCGCGGAG AACGGGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304932.1
CDS	102..512
Translation	MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYHPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_001318003.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus H3.4 histone (H3f4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318003.2

ORF Insert Sequence:

ATGGCACGCA CCAAGCAGAC GGCACGGAAG TCGACGGGAG GCAAGGCTCC GCGCAAGCAG 
CTAGCCACGA AGGTGGCCCG CAAGAGCGCC CCGGCCACCG GGGGCGTGAA GAAGCCGCAC 
CGCTACCACC CTGGCACGGT GGCGCTGCGC GAGATCCGGC GCTACCAGAA GTCCACCGAG 
CTGCTGATTC GCAAGTTGCC ATTCCAGCGC TTGGTGCGTG AGATCGCCCA GGACTTCAAG 
ACCGACCTGC GCTTCCAGAG CTCCGCGGTG ATGGCGCTGC AGGAGGCCTG CGAGTCGTAC 
CTCGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCTATCC ACGCCAAGCG GGTGACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGGCGC ATCCGCGGAG AACGGGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis.
Cell reports18(3)593-600(2017 01)
Ueda J,Harada A,Urahama T,Machida S,Maehara K,Hada M,Makino Y,Nogami J,Horikoshi N,Osakabe A,Taguchi H,Tanaka H,Tachiwana H,Yao T,Yamada M,Iwamoto T,Isotani A,Ikawa M,Tachibana T,Okada Y,Kimura H,Ohkawa Y,Kurumizaka H,Yamagata K

Tissue-specific expression of histone H3 variants diversified after species separation.
Epigenetics & chromatin835(2015)
Maehara K,Harada A,Sato Y,Matsumoto M,Nakayama KI,Kimura H,Ohkawa Y

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
The Journal of cell biology176(3)283-94(2007 Jan)
Govin J,Escoffier E,Rousseaux S,Kuhn L,Ferro M,Th?venon J,Catena R,Davidson I,Garin J,Khochbin S,Caron C

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H3f4 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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