Rc3h1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rc3h1
Entrez Gene ID	381305
Full Name	RING CCCH (C3H) domains 1
Synonyms	5730557L09Rik,Gm551,N28103,mKIAA2025
General protein information	Preferred Names RING CCCH (C3H) domains 1 Names roquin-1 RING finger and C3H zinc finger protein 1 RING finger and CCCH-type zinc finger domain-containing protein 1 probable E3 ubiquitin-protein ligase Roquin protein Sanroque roquin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024952.2	NP_001020123.1	roquin-1
XM_006496908.2	XP_006496971.1	roquin-1 isoform X1
XM_006496908.3	XP_006496971.1	roquin-1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RC3H1	XP_001234605.2
Homo sapiens (human)	RC3H1	NP_742068.1
Pan troglodytes (chimpanzee)	RC3H1	XP_001151649.2
	RC3H1	XP_001102746.1
Canis lupus familiaris (dog)	RC3H1	XP_537186.2
Mus musculus (house mouse)	Rc3h1	NP_001020123.1
Danio rerio (zebrafish)	si:dkey-60b12.1	NP_001108155.1
Xenopus tropicalis (tropical clawed frog)	rc3h1	XP_002931481.1

Gene Ontology
Bibliography

Related articles in PubMed

Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.
Rehage N, Davydova E, Conrad C, Behrens G, Maiser A, Stehklein JE, Brenner S, Klein J, Jeridi A, Hoffmann A, Lee E, Dianzani U, Willemsen R, Feederle R, Reiche K, Hackerm?ller J, Leonhardt H, Sharma S, Niessing D, Heissmeyer V
Nature communications9(1)299(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Roquin-mediated control of PI3K-mTOR signaling prevents autoimmunity by restraining activation and differentiation of conventional T cells and specialization of Treg cells.
Title: Roquin Suppresses the PI3K-mTOR Signaling Pathway to Inhibit T Helper Cell Differentiation and Conversion of Treg to Tfr Cells.

mutations of both Regnase-1 and Roquin in T cells leads to massive lymphocyte activation and increased TH1 cells. In contrast, mutation of either Regnase-1 or Roquin affected T cell activation to a lesser extent than the double mutation, indicating that Regnase-1 and Roquin function nonredundantly in T cells. Regnase-1 is capable of repressing Roquin mRNA.
Title: Regnase-1 and Roquin Nonredundantly Regulate Th1 Differentiation Causing Cardiac Inflammation and Fibrosis.

these studies suggest that modulation of Roquin in myeloid cells may reduce both inflammation and bacterial growth during the chronic phase of Mycobacterium tuberculosis infection
Title: Modulation of Roquin Function in Myeloid Cells Reduces

Crystal structures and NMR data show that the Roquin-1 ROQ domain recognizes hexaloops in the SELEX-derived alternative decay element (ADE) and in an ADE-like variant present in the Ox40 3'-UTR with identical binding modes.
Title: Roquin recognizes a non-canonical hexaloop structure in the 3'-UTR of Ox40.

RC3H1 is a multifunctional regulator of immune homeostasis. (Review)
Title: Roquin--a multifunctional regulator of immune homeostasis.

The following Rc3h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rc3h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu28798	XM_006496908.3 Latest version!	Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu11896	NM_001024952.2 Latest version!	Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu28798	XM_006496908.2	Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rc3h1 ( XM_006496908.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496971.1 Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28798
Clone ID Related Accession (Same CDS sequence)	XM_006496908.2 , XM_006496908.3
Accession Version	XM_006496908.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roquin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATTGT ACAGTCCTAG ATTTCAAATT GCTATGGAAG CTGGCTTAAG GATTGCACCA 
GACCAGTGGT CCTCTTTGCT TTATGGGGAT CAGCCTCACA AATCTCATAT GCAGTCTATT 
ATTGACAAGT TACAGACCCC TGCCTCATTT GCACAGAGTG TTCAGGAGCT AACAATTGCT 
CTCCAGCGGA CTGGAGACCC AGCGAATTTG AACCGACTAA GACCCCATTT GGAGTTACTC 
GCAAACATTG ACCCTAGTCC AGATGCTCCT CCTCCAACAT GGGAACAGCT AGAAAATGGG 
CTGGTTGCTG TACGTACGGT AGTGCATGGA TTGGTTGATT ATATCCAGAA CCACAGCAAA 
AAAGGAGCAG ATCAACAACA GCCTCCACAG CATAGCAAAT ACAAAACATA CATGTGTCGA 
GATATGAAGC AAAGGGGAGG ATGCCCTCGT GGTGCCAGCT GTACGTTTGC ACATTCACAG 
GAAGAACTGG AAAAGTTTCG TAAAATGAAC AAACGTCTGG TTCCCAGAAG ACCCTTGAGT 
GCCTCTTTGG GTCAACTTAA TGAGGTGGGC CTGCCTTCAG CACCTATACT TTCTGATGAA 
AGTGCAGTGG ACTTGTCCAA CAGAAAACCT CCTGCACTCC CAAATGGAAT TGCATCGTCA 
GGGAGCACAG TGACACAACT GATTCCACGT GGGACAGACC CCAGCTTTGA TTCTTCTCTG 
AAACCAGTAA AGCTAGACCA CCTGAGCAGT AGTGCTCCGG GGTCCCCTCC TGACCTGCTG 
GAATCTGCCC CTAAGAGTAT TTCTGCCTTA CCTGTGAATC CACATCCTGT ACCTCCAAGG 
GGACCAACAG ATCTGCCTCC CATGCCTGTC ACCAAACCAA TTCAGATGGT ACCTCGAGGT 
TCTCAGCTAT ATCCAGCACA ACAAGCAGAT GTTTATTATC AGGATCCTCG AGGAAGTGCC 
CCAGCTTTTG AGACAGCACC TTACCAGCAG GGTATGTACT ATACTCCACC ACCATGTGTG 
TCCCGCTTTG TCCGACCTCC ACCATCTGCT CCTGAACCTG GTCCTCCATA CTTGGATCAT 
TATTCACCCT ACCTCCAAGA TCGTGTCATA AACTCTCAGT ATGGCACACA GCCACAACAG 
TACCCACCTA TGTACCCAGC TCACTACGAT GGCCGCCGTG TATACCCTGC TCAGTCTTAC 
ACAAGAGAGG AGATGTTCCG GGAAAGCCCT ATACCCATTG ACATTCCATC TGCAGCAGTG 
CCGTCCTATG TACCTGAGTC CAGAGAACGG TACCAACAGG TAGAGGGTTA CTACCCGGTG 
GCTCCTCATC CAGCTCAGAT CAGACCTTCA TACCCCAGGG ATCCTCCTTA TAGTCGGCTT 
CCTCCTCCCC AACCCCATCC TAGTCTGGAT GAGCTACATC GAAGGCGGAA GGAAATAATG 
GCCCAGCTAG AGGAAAGAAA AGTCATCTCT CCACCTCCTT TTGCACCGTC ACCAACATTG 
CCTCCTGCAT TCCATCCAGA GGAATTTTTG GATGAAGACT TGAAGGTAGC CGGAAAATAC 
AAAGCAAATG ATTATAGCCA GTATTCTCCT TGGTCATGTG ATACCATCGG CTCCTACATT 
GGAACCAAAG ATGCAAAACC CAAAGATGTT GTGGCAGCGG GGAGTGTGGA AATGATGAAT 
GTAGAAAGTA AAGGAACGAG AGAGCAGAGG TTGGACCTTC AGAGAAGAGC AGTGGAGACG 
AGCGATGATG ACCTCATCCC GTTTGGAGAC CGACCAACAG TCTCTCGATT CGGTGCCATC 
TCTCGAACAT CCAAAACACT GTATCAGGGT GCTGGCCCAC TGCAAGCTAT AGCACCTCAG 
GGAGCACCCA CAAAATCTAT TAACATTTCC GATTACAGTG CATATGGAGC TCATGGTGGC 
TGGGGAGATT CTCCATACTC ACCTCATGCA AACATACCTC CTCAGGGACA CTTCATTGAG 
AGGGAGAAAA TGTCCATGGC AGAAGTGGCC AGTCATGGAA AACCCCTTCT ATCTGCTGAA 
AGAGAACAAT TACGGCTAGA ATTGCAGCAG CTGAACCATC AGATCAGCCA GCAGACCCAG 
CTACGAGGAC TAGAGGCTGT TAGTAACCGG CTGGTGTTAC AGAGGGAGGT GAACACCCTG 
GCAAGCCAGC CACAGCCCCC TCAGCTGCCT CCAAAATGGC CTGGAATGAT CTCAAGTGAG 
CAGCTCAGCT TGGAACTGCA TCAGGTGGAA AGAGAAATTG GGAAGAGGAC CCGGGAATTG 
AGTATGGAGA ACCAGTGTTC TGTAGACATG AAAAGCAAAC TGGGTACAAG CAAGCAAGCA 
GAAAATGGCC AGCCCGAGCC CCAAAACAAG ATCCGCACTG AGGACCTTAC ATTGACATTC 
AGTGATGTAC CAAATGGATC AGCCTTGACA CAAGAGAATC TCAGCCTCCT ATCAAACAAG 
ACCAGCTCTT TGAATCTGTC AGAAGACTCT GAGGGAGGAG GAGATAACAA TGATTCCCAG 
AGATCAGGAG TTGTTTCCAA TTCTGCTCCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496971.1
CDS	162..2714
Translation	MQLYSPRFQIAMEAGLRIAPDQWSSLLYGDQPHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLVDYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPSAPILSDESAVDLSNRKPPALPNGIASSGSTVTQLIPRGTDPSFDSSLKPVKLDHLSSSAPGSPPDLLESAPKSISALPVNPHPVPPRGPTDLPPMPVTKPIQMVPRGSQLYPAQQADVYYQDPRGSAPAFETAPYQQGMYYTPPPCVSRFVRPPPSAPEPGPPYLDHYSPYLQDRVINSQYGTQPQQYPPMYPAHYDGRRVYPAQSYTREEMFRESPIPIDIPSAAVPSYVPESRERYQQVEGYYPVAPHPAQIRPSYPRDPPYSRLPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPAFHPEEFLDEDLKVAGKYKANDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGTREQRLDLQRRAVETSDDDLIPFGDRPTVSRFGAISRTSKTLYQGAGPLQAIAPQGAPTKSINISDYSAYGAHGGWGDSPYSPHANIPPQGHFIEREKMSMAEVASHGKPLLSAEREQLRLELQQLNHQISQQTQLRGLEAVSNRLVLQREVNTLASQPQPPQLPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSVDMKSKLGTSKQAENGQPEPQNKIRTEDLTLTFSDVPNGSALTQENLSLLSNKTSSLNLSEDSEGGGDNNDSQRSGVVSNSAP

Target ORF information:

RefSeq Version	XM_006496908.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496908.2

ORF Insert Sequence:

ATGCAATTGT ACAGTCCTAG ATTTCAAATT GCTATGGAAG CTGGCTTAAG GATTGCACCA 
GACCAGTGGT CCTCTTTGCT TTATGGGGAT CAGCCTCACA AATCTCATAT GCAGTCTATT 
ATTGACAAGT TACAGACCCC TGCCTCATTT GCACAGAGTG TTCAGGAGCT AACAATTGCT 
CTCCAGCGGA CTGGAGACCC AGCGAATTTG AACCGACTAA GACCCCATTT GGAGTTACTC 
GCAAACATTG ACCCTAGTCC AGATGCTCCT CCTCCAACAT GGGAACAGCT AGAAAATGGG 
CTGGTTGCTG TACGTACGGT AGTGCATGGA TTGGTTGATT ATATCCAGAA CCACAGCAAA 
AAAGGAGCAG ATCAACAACA GCCTCCACAG CATAGCAAAT ACAAAACATA CATGTGTCGA 
GATATGAAGC AAAGGGGAGG ATGCCCTCGT GGTGCCAGCT GTACGTTTGC ACATTCACAG 
GAAGAACTGG AAAAGTTTCG TAAAATGAAC AAACGTCTGG TTCCCAGAAG ACCCTTGAGT 
GCCTCTTTGG GTCAACTTAA TGAGGTGGGC CTGCCTTCAG CACCTATACT TTCTGATGAA 
AGTGCAGTGG ACTTGTCCAA CAGAAAACCT CCTGCACTCC CAAATGGAAT TGCATCGTCA 
GGGAGCACAG TGACACAACT GATTCCACGT GGGACAGACC CCAGCTTTGA TTCTTCTCTG 
AAACCAGTAA AGCTAGACCA CCTGAGCAGT AGTGCTCCGG GGTCCCCTCC TGACCTGCTG 
GAATCTGCCC CTAAGAGTAT TTCTGCCTTA CCTGTGAATC CACATCCTGT ACCTCCAAGG 
GGACCAACAG ATCTGCCTCC CATGCCTGTC ACCAAACCAA TTCAGATGGT ACCTCGAGGT 
TCTCAGCTAT ATCCAGCACA ACAAGCAGAT GTTTATTATC AGGATCCTCG AGGAAGTGCC 
CCAGCTTTTG AGACAGCACC TTACCAGCAG GGTATGTACT ATACTCCACC ACCATGTGTG 
TCCCGCTTTG TCCGACCTCC ACCATCTGCT CCTGAACCTG GTCCTCCATA CTTGGATCAT 
TATTCACCCT ACCTCCAAGA TCGTGTCATA AACTCTCAGT ATGGCACACA GCCACAACAG 
TACCCACCTA TGTACCCAGC TCACTACGAT GGCCGCCGTG TATACCCTGC TCAGTCTTAC 
ACAAGAGAGG AGATGTTCCG GGAAAGCCCT ATACCCATTG ACATTCCATC TGCAGCAGTG 
CCGTCCTATG TACCTGAGTC CAGAGAACGG TACCAACAGG TAGAGGGTTA CTACCCGGTG 
GCTCCTCATC CAGCTCAGAT CAGACCTTCA TACCCCAGGG ATCCTCCTTA TAGTCGGCTT 
CCTCCTCCCC AACCCCATCC TAGTCTGGAT GAGCTACATC GAAGGCGGAA GGAAATAATG 
GCCCAGCTAG AGGAAAGAAA AGTCATCTCT CCACCTCCTT TTGCACCGTC ACCAACATTG 
CCTCCTGCAT TCCATCCAGA GGAATTTTTG GATGAAGACT TGAAGGTAGC CGGAAAATAC 
AAAGCAAATG ATTATAGCCA GTATTCTCCT TGGTCATGTG ATACCATCGG CTCCTACATT 
GGAACCAAAG ATGCAAAACC CAAAGATGTT GTGGCAGCGG GGAGTGTGGA AATGATGAAT 
GTAGAAAGTA AAGGAACGAG AGAGCAGAGG TTGGACCTTC AGAGAAGAGC AGTGGAGACG 
AGCGATGATG ACCTCATCCC GTTTGGAGAC CGACCAACAG TCTCTCGATT CGGTGCCATC 
TCTCGAACAT CCAAAACACT GTATCAGGGT GCTGGCCCAC TGCAAGCTAT AGCACCTCAG 
GGAGCACCCA CAAAATCTAT TAACATTTCC GATTACAGTG CATATGGAGC TCATGGTGGC 
TGGGGAGATT CTCCATACTC ACCTCATGCA AACATACCTC CTCAGGGACA CTTCATTGAG 
AGGGAGAAAA TGTCCATGGC AGAAGTGGCC AGTCATGGAA AACCCCTTCT ATCTGCTGAA 
AGAGAACAAT TACGGCTAGA ATTGCAGCAG CTGAACCATC AGATCAGCCA GCAGACCCAG 
CTACGAGGAC TAGAGGCTGT TAGTAACCGG CTGGTGTTAC AGAGGGAGGT GAACACCCTG 
GCAAGCCAGC CACAGCCCCC TCAGCTGCCT CCAAAATGGC CTGGAATGAT CTCAAGTGAG 
CAGCTCAGCT TGGAACTGCA TCAGGTGGAA AGAGAAATTG GGAAGAGGAC CCGGGAATTG 
AGTATGGAGA ACCAGTGTTC TGTAGACATG AAAAGCAAAC TGGGTACAAG CAAGCAAGCA 
GAAAATGGCC AGCCCGAGCC CCAAAACAAG ATCCGCACTG AGGACCTTAC ATTGACATTC 
AGTGATGTAC CAAATGGATC AGCCTTGACA CAAGAGAATC TCAGCCTCCT ATCAAACAAG 
ACCAGCTCTT TGAATCTGTC AGAAGACTCT GAGGGAGGAG GAGATAACAA TGATTCCCAG 
AGATCAGGAG TTGTTTCCAA TTCTGCTCCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h1 ( XM_006496908.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496971.1 Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28798
Clone ID Related Accession (Same CDS sequence)	XM_006496908.2 , XM_006496908.3
Accession Version	XM_006496908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roquin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATTGT ACAGTCCTAG ATTTCAAATT GCTATGGAAG CTGGCTTAAG GATTGCACCA 
GACCAGTGGT CCTCTTTGCT TTATGGGGAT CAGCCTCACA AATCTCATAT GCAGTCTATT 
ATTGACAAGT TACAGACCCC TGCCTCATTT GCACAGAGTG TTCAGGAGCT AACAATTGCT 
CTCCAGCGGA CTGGAGACCC AGCGAATTTG AACCGACTAA GACCCCATTT GGAGTTACTC 
GCAAACATTG ACCCTAGTCC AGATGCTCCT CCTCCAACAT GGGAACAGCT AGAAAATGGG 
CTGGTTGCTG TACGTACGGT AGTGCATGGA TTGGTTGATT ATATCCAGAA CCACAGCAAA 
AAAGGAGCAG ATCAACAACA GCCTCCACAG CATAGCAAAT ACAAAACATA CATGTGTCGA 
GATATGAAGC AAAGGGGAGG ATGCCCTCGT GGTGCCAGCT GTACGTTTGC ACATTCACAG 
GAAGAACTGG AAAAGTTTCG TAAAATGAAC AAACGTCTGG TTCCCAGAAG ACCCTTGAGT 
GCCTCTTTGG GTCAACTTAA TGAGGTGGGC CTGCCTTCAG CACCTATACT TTCTGATGAA 
AGTGCAGTGG ACTTGTCCAA CAGAAAACCT CCTGCACTCC CAAATGGAAT TGCATCGTCA 
GGGAGCACAG TGACACAACT GATTCCACGT GGGACAGACC CCAGCTTTGA TTCTTCTCTG 
AAACCAGTAA AGCTAGACCA CCTGAGCAGT AGTGCTCCGG GGTCCCCTCC TGACCTGCTG 
GAATCTGCCC CTAAGAGTAT TTCTGCCTTA CCTGTGAATC CACATCCTGT ACCTCCAAGG 
GGACCAACAG ATCTGCCTCC CATGCCTGTC ACCAAACCAA TTCAGATGGT ACCTCGAGGT 
TCTCAGCTAT ATCCAGCACA ACAAGCAGAT GTTTATTATC AGGATCCTCG AGGAAGTGCC 
CCAGCTTTTG AGACAGCACC TTACCAGCAG GGTATGTACT ATACTCCACC ACCATGTGTG 
TCCCGCTTTG TCCGACCTCC ACCATCTGCT CCTGAACCTG GTCCTCCATA CTTGGATCAT 
TATTCACCCT ACCTCCAAGA TCGTGTCATA AACTCTCAGT ATGGCACACA GCCACAACAG 
TACCCACCTA TGTACCCAGC TCACTACGAT GGCCGCCGTG TATACCCTGC TCAGTCTTAC 
ACAAGAGAGG AGATGTTCCG GGAAAGCCCT ATACCCATTG ACATTCCATC TGCAGCAGTG 
CCGTCCTATG TACCTGAGTC CAGAGAACGG TACCAACAGG TAGAGGGTTA CTACCCGGTG 
GCTCCTCATC CAGCTCAGAT CAGACCTTCA TACCCCAGGG ATCCTCCTTA TAGTCGGCTT 
CCTCCTCCCC AACCCCATCC TAGTCTGGAT GAGCTACATC GAAGGCGGAA GGAAATAATG 
GCCCAGCTAG AGGAAAGAAA AGTCATCTCT CCACCTCCTT TTGCACCGTC ACCAACATTG 
CCTCCTGCAT TCCATCCAGA GGAATTTTTG GATGAAGACT TGAAGGTAGC CGGAAAATAC 
AAAGCAAATG ATTATAGCCA GTATTCTCCT TGGTCATGTG ATACCATCGG CTCCTACATT 
GGAACCAAAG ATGCAAAACC CAAAGATGTT GTGGCAGCGG GGAGTGTGGA AATGATGAAT 
GTAGAAAGTA AAGGAACGAG AGAGCAGAGG TTGGACCTTC AGAGAAGAGC AGTGGAGACG 
AGCGATGATG ACCTCATCCC GTTTGGAGAC CGACCAACAG TCTCTCGATT CGGTGCCATC 
TCTCGAACAT CCAAAACACT GTATCAGGGT GCTGGCCCAC TGCAAGCTAT AGCACCTCAG 
GGAGCACCCA CAAAATCTAT TAACATTTCC GATTACAGTG CATATGGAGC TCATGGTGGC 
TGGGGAGATT CTCCATACTC ACCTCATGCA AACATACCTC CTCAGGGACA CTTCATTGAG 
AGGGAGAAAA TGTCCATGGC AGAAGTGGCC AGTCATGGAA AACCCCTTCT ATCTGCTGAA 
AGAGAACAAT TACGGCTAGA ATTGCAGCAG CTGAACCATC AGATCAGCCA GCAGACCCAG 
CTACGAGGAC TAGAGGCTGT TAGTAACCGG CTGGTGTTAC AGAGGGAGGT GAACACCCTG 
GCAAGCCAGC CACAGCCCCC TCAGCTGCCT CCAAAATGGC CTGGAATGAT CTCAAGTGAG 
CAGCTCAGCT TGGAACTGCA TCAGGTGGAA AGAGAAATTG GGAAGAGGAC CCGGGAATTG 
AGTATGGAGA ACCAGTGTTC TGTAGACATG AAAAGCAAAC TGGGTACAAG CAAGCAAGCA 
GAAAATGGCC AGCCCGAGCC CCAAAACAAG ATCCGCACTG AGGACCTTAC ATTGACATTC 
AGTGATGTAC CAAATGGATC AGCCTTGACA CAAGAGAATC TCAGCCTCCT ATCAAACAAG 
ACCAGCTCTT TGAATCTGTC AGAAGACTCT GAGGGAGGAG GAGATAACAA TGATTCCCAG 
AGATCAGGAG TTGTTTCCAA TTCTGCTCCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496971.1
CDS	793..3345
Translation	MQLYSPRFQIAMEAGLRIAPDQWSSLLYGDQPHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLVDYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPSAPILSDESAVDLSNRKPPALPNGIASSGSTVTQLIPRGTDPSFDSSLKPVKLDHLSSSAPGSPPDLLESAPKSISALPVNPHPVPPRGPTDLPPMPVTKPIQMVPRGSQLYPAQQADVYYQDPRGSAPAFETAPYQQGMYYTPPPCVSRFVRPPPSAPEPGPPYLDHYSPYLQDRVINSQYGTQPQQYPPMYPAHYDGRRVYPAQSYTREEMFRESPIPIDIPSAAVPSYVPESRERYQQVEGYYPVAPHPAQIRPSYPRDPPYSRLPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPAFHPEEFLDEDLKVAGKYKANDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGTREQRLDLQRRAVETSDDDLIPFGDRPTVSRFGAISRTSKTLYQGAGPLQAIAPQGAPTKSINISDYSAYGAHGGWGDSPYSPHANIPPQGHFIEREKMSMAEVASHGKPLLSAEREQLRLELQQLNHQISQQTQLRGLEAVSNRLVLQREVNTLASQPQPPQLPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSVDMKSKLGTSKQAENGQPEPQNKIRTEDLTLTFSDVPNGSALTQENLSLLSNKTSSLNLSEDSEGGGDNNDSQRSGVVSNSAP

Target ORF information:

RefSeq Version	XM_006496908.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496908.3

ORF Insert Sequence:

ATGCAATTGT ACAGTCCTAG ATTTCAAATT GCTATGGAAG CTGGCTTAAG GATTGCACCA 
GACCAGTGGT CCTCTTTGCT TTATGGGGAT CAGCCTCACA AATCTCATAT GCAGTCTATT 
ATTGACAAGT TACAGACCCC TGCCTCATTT GCACAGAGTG TTCAGGAGCT AACAATTGCT 
CTCCAGCGGA CTGGAGACCC AGCGAATTTG AACCGACTAA GACCCCATTT GGAGTTACTC 
GCAAACATTG ACCCTAGTCC AGATGCTCCT CCTCCAACAT GGGAACAGCT AGAAAATGGG 
CTGGTTGCTG TACGTACGGT AGTGCATGGA TTGGTTGATT ATATCCAGAA CCACAGCAAA 
AAAGGAGCAG ATCAACAACA GCCTCCACAG CATAGCAAAT ACAAAACATA CATGTGTCGA 
GATATGAAGC AAAGGGGAGG ATGCCCTCGT GGTGCCAGCT GTACGTTTGC ACATTCACAG 
GAAGAACTGG AAAAGTTTCG TAAAATGAAC AAACGTCTGG TTCCCAGAAG ACCCTTGAGT 
GCCTCTTTGG GTCAACTTAA TGAGGTGGGC CTGCCTTCAG CACCTATACT TTCTGATGAA 
AGTGCAGTGG ACTTGTCCAA CAGAAAACCT CCTGCACTCC CAAATGGAAT TGCATCGTCA 
GGGAGCACAG TGACACAACT GATTCCACGT GGGACAGACC CCAGCTTTGA TTCTTCTCTG 
AAACCAGTAA AGCTAGACCA CCTGAGCAGT AGTGCTCCGG GGTCCCCTCC TGACCTGCTG 
GAATCTGCCC CTAAGAGTAT TTCTGCCTTA CCTGTGAATC CACATCCTGT ACCTCCAAGG 
GGACCAACAG ATCTGCCTCC CATGCCTGTC ACCAAACCAA TTCAGATGGT ACCTCGAGGT 
TCTCAGCTAT ATCCAGCACA ACAAGCAGAT GTTTATTATC AGGATCCTCG AGGAAGTGCC 
CCAGCTTTTG AGACAGCACC TTACCAGCAG GGTATGTACT ATACTCCACC ACCATGTGTG 
TCCCGCTTTG TCCGACCTCC ACCATCTGCT CCTGAACCTG GTCCTCCATA CTTGGATCAT 
TATTCACCCT ACCTCCAAGA TCGTGTCATA AACTCTCAGT ATGGCACACA GCCACAACAG 
TACCCACCTA TGTACCCAGC TCACTACGAT GGCCGCCGTG TATACCCTGC TCAGTCTTAC 
ACAAGAGAGG AGATGTTCCG GGAAAGCCCT ATACCCATTG ACATTCCATC TGCAGCAGTG 
CCGTCCTATG TACCTGAGTC CAGAGAACGG TACCAACAGG TAGAGGGTTA CTACCCGGTG 
GCTCCTCATC CAGCTCAGAT CAGACCTTCA TACCCCAGGG ATCCTCCTTA TAGTCGGCTT 
CCTCCTCCCC AACCCCATCC TAGTCTGGAT GAGCTACATC GAAGGCGGAA GGAAATAATG 
GCCCAGCTAG AGGAAAGAAA AGTCATCTCT CCACCTCCTT TTGCACCGTC ACCAACATTG 
CCTCCTGCAT TCCATCCAGA GGAATTTTTG GATGAAGACT TGAAGGTAGC CGGAAAATAC 
AAAGCAAATG ATTATAGCCA GTATTCTCCT TGGTCATGTG ATACCATCGG CTCCTACATT 
GGAACCAAAG ATGCAAAACC CAAAGATGTT GTGGCAGCGG GGAGTGTGGA AATGATGAAT 
GTAGAAAGTA AAGGAACGAG AGAGCAGAGG TTGGACCTTC AGAGAAGAGC AGTGGAGACG 
AGCGATGATG ACCTCATCCC GTTTGGAGAC CGACCAACAG TCTCTCGATT CGGTGCCATC 
TCTCGAACAT CCAAAACACT GTATCAGGGT GCTGGCCCAC TGCAAGCTAT AGCACCTCAG 
GGAGCACCCA CAAAATCTAT TAACATTTCC GATTACAGTG CATATGGAGC TCATGGTGGC 
TGGGGAGATT CTCCATACTC ACCTCATGCA AACATACCTC CTCAGGGACA CTTCATTGAG 
AGGGAGAAAA TGTCCATGGC AGAAGTGGCC AGTCATGGAA AACCCCTTCT ATCTGCTGAA 
AGAGAACAAT TACGGCTAGA ATTGCAGCAG CTGAACCATC AGATCAGCCA GCAGACCCAG 
CTACGAGGAC TAGAGGCTGT TAGTAACCGG CTGGTGTTAC AGAGGGAGGT GAACACCCTG 
GCAAGCCAGC CACAGCCCCC TCAGCTGCCT CCAAAATGGC CTGGAATGAT CTCAAGTGAG 
CAGCTCAGCT TGGAACTGCA TCAGGTGGAA AGAGAAATTG GGAAGAGGAC CCGGGAATTG 
AGTATGGAGA ACCAGTGTTC TGTAGACATG AAAAGCAAAC TGGGTACAAG CAAGCAAGCA 
GAAAATGGCC AGCCCGAGCC CCAAAACAAG ATCCGCACTG AGGACCTTAC ATTGACATTC 
AGTGATGTAC CAAATGGATC AGCCTTGACA CAAGAGAATC TCAGCCTCCT ATCAAACAAG 
ACCAGCTCTT TGAATCTGTC AGAAGACTCT GAGGGAGGAG GAGATAACAA TGATTCCCAG 
AGATCAGGAG TTGTTTCCAA TTCTGCTCCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h1 ( NM_001024952.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001020123.1 Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), mRNA.

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CloneID	OMu11896
Clone ID Related Accession (Same CDS sequence)	NM_001024952.2
Accession Version	NM_001024952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	roquin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ133431.1, AY948287.1 and AC119204.7. On Oct 4, 2007 this sequence version replaced NM_001024952.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173335.1, AK139648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGTAC AAGCTCCACA ATGGACGGAT TTCCTTTCCT GCCCAATTTG CACTCAGACT 
TTCGACGAAA CAATTCGAAA GCCCATCAGT TTGGGCTGTG GCCATACTGT CTGCAAAATG 
TGCCTGAATA AACTCCACCG TAAGGCTTGC CCATTTGACC AGACCACTAT CAATACGGAC 
ATTGAGCTTC TTCCTGTGAA CTCAGCATTA CTGCAGCTAG TGGGTGCTCA GATCCCTGAG 
CAACAGCCCA TTACTTTGTG TAGTGGGGTT GAAGATACAA AGCATTATGA GGAAGCCAAG 
AAATGTGTAG AAGAATTAGC ATTGTACCTG AAGCCACTCA GCAGTGCGAG AGGTGTGGGT 
CTAAATAGCA CTACTCAGAG TGTTCTGAGT CGCCCAATGC AGAGGAAACT TGTGACTCTG 
GTTCATTGCC AGCTAGTGGA AGAAGAAGGC AGGATCCGTG CTATGAGGGC TGCTCGATCT 
TTAGGTGAAC GCACAGTTAC AGAGCTCATT CTCCAGCACC AGAATCCTCA GCAACTCTCT 
TCTAATCTCT GGGCAGCAGT CAGAGCTAGG GGCTGCCAGT TCCTTGGACC AGCGATGCAG 
GAGGAAGCTC TGAAGCTGGT CTTGCTGGCC TTAGAAGATG GTTCTGCATT GTCTCGGAAA 
GTGTTGGTTC TCTTCGTGGT GCAAAGACTG GAGCCACGTT TTCCTCAAGC CTCTAAAACC 
AGCATTGGGC ATGTTGTTCA GCTCCTCTAC AGAGCCTCCT GCTTCAAGGT CACCAAACGT 
GATGAAGACT CTTCTTTGAT GCAACTGAAA GAAGAATTTA GGACTTACGA AGCTCTGAGG 
AGAGAACATG ACTCCCAGAT AGTGCAAATT GCTATGGAAG CTGGCTTAAG GATTGCACCA 
GACCAGTGGT CCTCTTTGCT TTATGGGGAT CAGTCTCACA AATCTCATAT GCAGTCTATT 
ATTGACAAGT TACAGACCCC TGCCTCATTT GCACAGAGTG TTCAGGAGCT AACAATTGCT 
CTCCAGCGGA CTGGAGACCC AGCGAATTTG AACCGACTAA GACCCCATTT GGAGTTACTC 
GCAAACATTG ACCCTAGTCC AGATGCTCCT CCTCCAACAT GGGAACAGCT AGAAAATGGG 
CTGGTTGCTG TACGTACGGT AGTGCATGGA TTGGTTGATT ATATCCAGAA CCACAGCAAA 
AAAGGAGCAG ATCAACAACA GCCTCCACAG CATAGCAAAT ACAAAACATA CATGTGTCGA 
GATATGAAGC AAAGGGGAGG ATGCCCTCGT GGTGCCAGCT GTACGTTTGC ACATTCACAG 
GAAGAACTGG AAAAGTTTCG TAAAATGAAC AAACGTCTGG TTCCCAGAAG ACCCTTGAGT 
GCCTCTTTGG GTCAACTTAA TGAGGTGGGC CTGCCTTCAG CACCTATACT TTCTGATGAA 
AGTGCAGTGG ACTTGTCCAA CAGAAAACCT CCTGCACTCC CAAATGGAAT TGCATCGTCA 
GGGAGCACAG TGACACAACT GATTCCACGT GGGACAGACC CCAGCTTTGA TTCTTCTCTG 
AAACCAGTAA AGCTAGACCA CCTGAGCAGT AGTGCTCCGG GGTCCCCTCC TGACCTGCTG 
GAATCTGCCC CTAAGAGTAT TTCTGCCTTA CCTGTGAATC CACATCCTGT ACCTCCAAGG 
GGACCAACAG ATCTGCCTCC CATGCCTGTC ACCAAACCAA TTCAGATGGT ACCTCGAGGT 
TCTCAGCTAT ATCCAGCACA ACAAGCAGAT GTTTATTATC AGGATCCTCG AGGAAGTGCC 
CCAGCTTTTG AGACAGCACC TTACCAGCAG GGTATGTACT ATACTCCACC ACCATGTGTG 
TCCCGCTTTG TCCGACCTCC ACCATCTGCT CCTGAACCTG GTCCTCCATA CTTGGATCAT 
TATTCACCCT ACCTCCAAGA TCGTGTCATA AACTCTCAGT ATGGCACACA GCCACAACAG 
TACCCACCTA TGTACCCAGC TCACTACGAT GGCCGCCGTG TATACCCTGC TCAGTCTTAC 
ACAAGAGAGG AGATGTTCCG GGAAAGCCCT ATACCCATTG ACATTCCATC TGCAGCAGTG 
CCGTCCTATG TACCTGAGTC CAGAGAACGG TACCAACAGG TAGAGGGTTA CTACCCGGTG 
GCTCCTCATC CAGCTCAGAT CAGACCTTCA TACCCCAGGG ATCCTCCTTA TAGTCGGCTT 
CCTCCTCCCC AACCCCATCC TAGTCTGGAT GAGCTACATC GAAGGCGGAA GGAAATAATG 
GCCCAGCTAG AGGAAAGAAA AGTCATCTCT CCACCTCCTT TTGCACCGTC ACCAACATTG 
CCTCCTGCAT TCCATCCAGA GGAATTTTTG GATGAAGACT TGAAGGTAGC CGGAAAATAC 
AAAGCAAATG ATTATAGCCA GTATTCTCCT TGGTCATGTG ATACCATCGG CTCCTACATT 
GGAACCAAAG ATGCAAAACC CAAAGATGTT GTGGCAGCGG GGAGTGTGGA AATGATGAAT 
GTAGAAAGTA AAGGAACGAG AGAGCAGAGG TTGGACCTTC AGAGAAGAGC AGTGGAGACG 
AGCGATGATG ACCTCATCCC GTTTGGAGAC CGACCAACAG TCTCTCGATT CGGTGCCATC 
TCTCGAACAT CCAAAACACT GTATCAGGGT GCTGGCCCAC TGCAAGCTAT AGCACCTCAG 
GGAGCACCCA CAAAATCTAT TAACATTTCC GATTACAGTG CATATGGAGC TCATGGTGGC 
TGGGGAGATT CTCCATACTC ACCTCATGCA AACATACCTC CTCAGGGACA CTTCATTGAG 
AGGGAGAAAA TGTCCATGGC AGAAGTGGCC AGTCATGGAA AACCCCTTCT ATCTGCTGAA 
AGAGAACAAT TACGGCTAGA ATTGCAGCAG CTGAACCATC AGATCAGCCA GCAGACCCAG 
CTACGAGGAC TAGAGGCTGT TAGTAACCGG CTGGTGTTAC AGAGGGAGGT GAACACCCTG 
GCAAGCCAGC CACAGCCCCC TCAGCTGCCT CCAAAATGGC CTGGAATGAT CTCAAGTGAG 
CAGCTCAGCT TGGAACTGCA TCAGGTGGAA AGAGAAATTG GGAAGAGGAC CCGGGAATTG 
AGTATGGAGA ACCAGTGTTC TGTAGACATG AAAAGCAAAC TGGGTACAAG CAAGCAAGCA 
GAAAATGGCC AGCCCGAGCC CCAAAACAAG ATCCGCACTG AGGACCTTAC ATTGACATTC 
AGTGATGTAC CAAATGGATC AGCCTTGACA CAAGAGAATC TCAGCCTCCT ATCAAACAAG 
ACCAGCTCTT TGAATCTGTC AGAAGACTCT GAGGGAGGAG GAGATAACAA TGATTCCCAG 
AGATCAGGAG TTGTTTCCAA TTCTGCTCCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020123.1
CDS	440..3832
Translation	MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQIPEQQPITLCSGVEDTKHYEEAKKCVEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLVDYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPSAPILSDESAVDLSNRKPPALPNGIASSGSTVTQLIPRGTDPSFDSSLKPVKLDHLSSSAPGSPPDLLESAPKSISALPVNPHPVPPRGPTDLPPMPVTKPIQMVPRGSQLYPAQQADVYYQDPRGSAPAFETAPYQQGMYYTPPPCVSRFVRPPPSAPEPGPPYLDHYSPYLQDRVINSQYGTQPQQYPPMYPAHYDGRRVYPAQSYTREEMFRESPIPIDIPSAAVPSYVPESRERYQQVEGYYPVAPHPAQIRPSYPRDPPYSRLPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPAFHPEEFLDEDLKVAGKYKANDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGTREQRLDLQRRAVETSDDDLIPFGDRPTVSRFGAISRTSKTLYQGAGPLQAIAPQGAPTKSINISDYSAYGAHGGWGDSPYSPHANIPPQGHFIEREKMSMAEVASHGKPLLSAEREQLRLELQQLNHQISQQTQLRGLEAVSNRLVLQREVNTLASQPQPPQLPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSVDMKSKLGTSKQAENGQPEPQNKIRTEDLTLTFSDVPNGSALTQENLSLLSNKTSSLNLSEDSEGGGDNNDSQRSGVVSNSAP

Target ORF information:

RefSeq Version	NM_001024952.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024952.2

ORF Insert Sequence:

ATGCCTGTAC AAGCTCCACA ATGGACGGAT TTCCTTTCCT GCCCAATTTG CACTCAGACT 
TTCGACGAAA CAATTCGAAA GCCCATCAGT TTGGGCTGTG GCCATACTGT CTGCAAAATG 
TGCCTGAATA AACTCCACCG TAAGGCTTGC CCATTTGACC AGACCACTAT CAATACGGAC 
ATTGAGCTTC TTCCTGTGAA CTCAGCATTA CTGCAGCTAG TGGGTGCTCA GATCCCTGAG 
CAACAGCCCA TTACTTTGTG TAGTGGGGTT GAAGATACAA AGCATTATGA GGAAGCCAAG 
AAATGTGTAG AAGAATTAGC ATTGTACCTG AAGCCACTCA GCAGTGCGAG AGGTGTGGGT 
CTAAATAGCA CTACTCAGAG TGTTCTGAGT CGCCCAATGC AGAGGAAACT TGTGACTCTG 
GTTCATTGCC AGCTAGTGGA AGAAGAAGGC AGGATCCGTG CTATGAGGGC TGCTCGATCT 
TTAGGTGAAC GCACAGTTAC AGAGCTCATT CTCCAGCACC AGAATCCTCA GCAACTCTCT 
TCTAATCTCT GGGCAGCAGT CAGAGCTAGG GGCTGCCAGT TCCTTGGACC AGCGATGCAG 
GAGGAAGCTC TGAAGCTGGT CTTGCTGGCC TTAGAAGATG GTTCTGCATT GTCTCGGAAA 
GTGTTGGTTC TCTTCGTGGT GCAAAGACTG GAGCCACGTT TTCCTCAAGC CTCTAAAACC 
AGCATTGGGC ATGTTGTTCA GCTCCTCTAC AGAGCCTCCT GCTTCAAGGT CACCAAACGT 
GATGAAGACT CTTCTTTGAT GCAACTGAAA GAAGAATTTA GGACTTACGA AGCTCTGAGG 
AGAGAACATG ACTCCCAGAT AGTGCAAATT GCTATGGAAG CTGGCTTAAG GATTGCACCA 
GACCAGTGGT CCTCTTTGCT TTATGGGGAT CAGTCTCACA AATCTCATAT GCAGTCTATT 
ATTGACAAGT TACAGACCCC TGCCTCATTT GCACAGAGTG TTCAGGAGCT AACAATTGCT 
CTCCAGCGGA CTGGAGACCC AGCGAATTTG AACCGACTAA GACCCCATTT GGAGTTACTC 
GCAAACATTG ACCCTAGTCC AGATGCTCCT CCTCCAACAT GGGAACAGCT AGAAAATGGG 
CTGGTTGCTG TACGTACGGT AGTGCATGGA TTGGTTGATT ATATCCAGAA CCACAGCAAA 
AAAGGAGCAG ATCAACAACA GCCTCCACAG CATAGCAAAT ACAAAACATA CATGTGTCGA 
GATATGAAGC AAAGGGGAGG ATGCCCTCGT GGTGCCAGCT GTACGTTTGC ACATTCACAG 
GAAGAACTGG AAAAGTTTCG TAAAATGAAC AAACGTCTGG TTCCCAGAAG ACCCTTGAGT 
GCCTCTTTGG GTCAACTTAA TGAGGTGGGC CTGCCTTCAG CACCTATACT TTCTGATGAA 
AGTGCAGTGG ACTTGTCCAA CAGAAAACCT CCTGCACTCC CAAATGGAAT TGCATCGTCA 
GGGAGCACAG TGACACAACT GATTCCACGT GGGACAGACC CCAGCTTTGA TTCTTCTCTG 
AAACCAGTAA AGCTAGACCA CCTGAGCAGT AGTGCTCCGG GGTCCCCTCC TGACCTGCTG 
GAATCTGCCC CTAAGAGTAT TTCTGCCTTA CCTGTGAATC CACATCCTGT ACCTCCAAGG 
GGACCAACAG ATCTGCCTCC CATGCCTGTC ACCAAACCAA TTCAGATGGT ACCTCGAGGT 
TCTCAGCTAT ATCCAGCACA ACAAGCAGAT GTTTATTATC AGGATCCTCG AGGAAGTGCC 
CCAGCTTTTG AGACAGCACC TTACCAGCAG GGTATGTACT ATACTCCACC ACCATGTGTG 
TCCCGCTTTG TCCGACCTCC ACCATCTGCT CCTGAACCTG GTCCTCCATA CTTGGATCAT 
TATTCACCCT ACCTCCAAGA TCGTGTCATA AACTCTCAGT ATGGCACACA GCCACAACAG 
TACCCACCTA TGTACCCAGC TCACTACGAT GGCCGCCGTG TATACCCTGC TCAGTCTTAC 
ACAAGAGAGG AGATGTTCCG GGAAAGCCCT ATACCCATTG ACATTCCATC TGCAGCAGTG 
CCGTCCTATG TACCTGAGTC CAGAGAACGG TACCAACAGG TAGAGGGTTA CTACCCGGTG 
GCTCCTCATC CAGCTCAGAT CAGACCTTCA TACCCCAGGG ATCCTCCTTA TAGTCGGCTT 
CCTCCTCCCC AACCCCATCC TAGTCTGGAT GAGCTACATC GAAGGCGGAA GGAAATAATG 
GCCCAGCTAG AGGAAAGAAA AGTCATCTCT CCACCTCCTT TTGCACCGTC ACCAACATTG 
CCTCCTGCAT TCCATCCAGA GGAATTTTTG GATGAAGACT TGAAGGTAGC CGGAAAATAC 
AAAGCAAATG ATTATAGCCA GTATTCTCCT TGGTCATGTG ATACCATCGG CTCCTACATT 
GGAACCAAAG ATGCAAAACC CAAAGATGTT GTGGCAGCGG GGAGTGTGGA AATGATGAAT 
GTAGAAAGTA AAGGAACGAG AGAGCAGAGG TTGGACCTTC AGAGAAGAGC AGTGGAGACG 
AGCGATGATG ACCTCATCCC GTTTGGAGAC CGACCAACAG TCTCTCGATT CGGTGCCATC 
TCTCGAACAT CCAAAACACT GTATCAGGGT GCTGGCCCAC TGCAAGCTAT AGCACCTCAG 
GGAGCACCCA CAAAATCTAT TAACATTTCC GATTACAGTG CATATGGAGC TCATGGTGGC 
TGGGGAGATT CTCCATACTC ACCTCATGCA AACATACCTC CTCAGGGACA CTTCATTGAG 
AGGGAGAAAA TGTCCATGGC AGAAGTGGCC AGTCATGGAA AACCCCTTCT ATCTGCTGAA 
AGAGAACAAT TACGGCTAGA ATTGCAGCAG CTGAACCATC AGATCAGCCA GCAGACCCAG 
CTACGAGGAC TAGAGGCTGT TAGTAACCGG CTGGTGTTAC AGAGGGAGGT GAACACCCTG 
GCAAGCCAGC CACAGCCCCC TCAGCTGCCT CCAAAATGGC CTGGAATGAT CTCAAGTGAG 
CAGCTCAGCT TGGAACTGCA TCAGGTGGAA AGAGAAATTG GGAAGAGGAC CCGGGAATTG 
AGTATGGAGA ACCAGTGTTC TGTAGACATG AAAAGCAAAC TGGGTACAAG CAAGCAAGCA 
GAAAATGGCC AGCCCGAGCC CCAAAACAAG ATCCGCACTG AGGACCTTAC ATTGACATTC 
AGTGATGTAC CAAATGGATC AGCCTTGACA CAAGAGAATC TCAGCCTCCT ATCAAACAAG 
ACCAGCTCTT TGAATCTGTC AGAAGACTCT GAGGGAGGAG GAGATAACAA TGATTCCCAG 
AGATCAGGAG TTGTTTCCAA TTCTGCTCCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.
Nature communications9(1)299(2018 Jan)
Rehage N,Davydova E,Conrad C,Behrens G,Maiser A,Stehklein JE,Brenner S,Klein J,Jeridi A,Hoffmann A,Lee E,Dianzani U,Willemsen R,Feederle R,Reiche K,Hackerm?ller J,Leonhardt H,Sharma S,Niessing D,Heissmeyer V

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Rc3h1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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