Npw cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npw
Entrez Gene ID	381073
Full Name	neuropeptide W
Synonyms	Gm935
General protein information	Preferred Names neuropeptide W Names neuropeptide W
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A3.3
Summary	Orthologs of this gene express a preproprotein that is processed into a neuropeptide ligand for two G-protein coupled receptors in the central nervous system. The neuropeptide is thought to directly or indirectly regulate feeding, weight regulation, and pain response. The preproprotein is translated using a non-AUG initiation codon. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099664.1	NP_001093134.1	neuropeptide W preproprotein
NM_001099664.2	NP_001093134.1	neuropeptide W preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	NPW	NP_001092926.2
	NPW	XP_001082966.2
Rattus norvegicus (Norway rat)	Npw	NP_695206.1
Mus musculus (house mouse)	Npw	NP_001093134.1
Pan troglodytes (chimpanzee)	NPW	XP_003952809.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in?vitro and in?vivo.
Wang R, Yan F, Liao R, Wan P, Little PJ, Zheng W
Molecular and cellular endocrinology44771-78(2017 May)

Mesolimbic neuropeptide W coordinates stress responses under novel environments.
Motoike T, Long JM, Tanaka H, Sinton CM, Skach A, Williams SC, Hammer RE, Sakurai T, Yanagisawa M
Proceedings of the National Academy of Sciences of the United States of America113(21)6023-8(2016 May)

Neuropeptide W modulation of gastric vagal afferent mechanosensitivity: Impact of age and sex.
Li H, Frisby CL, O'Donnell TA, Kentish SJ, Wittert GA, Page AJ
Peptides71141-8(2015 Sep)

Transient expression of neuropeptide W in postnatal mouse hypothalamus--a putative regulator of energy homeostasis.
Motoike T, Skach AG, Godwin JK, Sinton CM, Yamazaki M, Abe M, Natsume R, Sakimura K, Yanagisawa M
Neuroscience301323-37(2015 Aug)

Gastric neuropeptide W is regulated by meal-related nutrients.
Li H, Feinle-Bisset C, Frisby C, Kentish S, Wittert GA, Page AJ
Peptides626-14(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the effect and underlying mechanisms of NGF/BDNF on the production of NPW in PC12 cells and hypothalamus, is reported.
Title: Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in?vitro and in?vivo.

These results indicate that the NPW/NPBWR1 system could play a critical role in the gating of stressful stimuli during exposure to novel environments.
Title: Mesolimbic neuropeptide W coordinates stress responses under novel environments.

These findings suggest that the physiological role of NPW varies depending on the age of the mice.
Title: Neuropeptide W modulation of gastric vagal afferent mechanosensitivity: Impact of age and sex.

These data suggest that the dorsomedial hypothalamus neurons that transiently co-express NPW and NPY in the peri-weaning period might play a role in regulating energy homeostasis during postnatal development.
Title: Transient expression of neuropeptide W in postnatal mouse hypothalamus--a putative regulator of energy homeostasis.

these studies indicated an inconsistency between plasma and gastric NPW expression in response to nutrient ingestion, suggesting food induced gastric NPW expression may play a more important role locally
Title: Gastric neuropeptide W is regulated by meal-related nutrients.

The following Npw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17775	NM_001099664.2 Latest version!	Mus musculus neuropeptide W (Npw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu17775	NM_001099664.1	Mus musculus neuropeptide W (Npw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npw ( NM_001099664.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001093134.1 Mus musculus neuropeptide W (Npw), mRNA.

Price & Availability↑

CloneID	OMu17775
Clone ID Related Accession (Same CDS sequence)	NM_001099664.1 , NM_001099664.2
Accession Version	NM_001099664.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Mus musculus(house mouse)
Product	neuropeptide W preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC154566.2 and AK131881.1. On or before Jul 25, 2007 this sequence version replaced XM_993080.1, XM_001478349.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by publication and orthologous data. ##Evidence-Data-START## Transcript exon combination :: AK131881.1, BY708553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 12719537 ##RefSeq-Attributes-END##

CTGGCGTCTA ACAGAGAAGT GCGGGGCCCT GGGCCCGGGA CTCCCAGGAA CCGGCCCCTG 
CTGCCCCTGC TGCTGCTTCT GCTCTTGCTA CCGCTGCCCG CCAGCGCCTG GTATAAGCAC 
GTGGCGAGTC CCCGCTATCA CACAGTGGGT CGTGCCTCCG GGCTGCTCAT GGGGCTGCGC 
CGCTCGCCCT ACCAGTGGCG CCGTGCCCTG GGCGGGGCTG CTGGACCCCT CTCCCGGCTC 
CCAGGACCGG TCGCCCGCGG CGCTCTCCTG CTTCCTTCCT CAGGGCAGGA GCTGTGGGAG 
GTACGAAGCA GGAGCTCACC TGCAGGGCTT CCCGTCCATG CACCCTGGAG TCCGCGGGAC 
CTGGAGGGAG TCCGCCAACC GGAGCAGTCG CTAAGCCTTC ACTCCTGGAT CTCAGAGGAG 
CCCGCTGCTA GAGCCTTCGG AGAGACGCTT CGTGCCCAGC CATGGTTCCT GCAGCAAGTC 
ATCTTTGCCG ATCCTGTCAG GCCCAAGAAC CGATGGCGCC CCCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093134.1
CDS	1..531
Translation	LASNREVRGPGPGTPRNRPLLPLLLLLLLLPLPASAWYKHVASPRYHTVGRASGLLMGLRRSPYQWRRALGGAAGPLSRLPGPVARGALLLPSSGQELWEVRSRSSPAGLPVHAPWSPRDLEGVRQPEQSLSLHSWISEEPAARAFGETLRAQPWFLQQVIFADPVRPKNRWRPHA

Target ORF information:

RefSeq Version	NM_001099664.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuropeptide W (Npw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099664.1

ORF Insert Sequence:

CTGGCGTCTA ACAGAGAAGT GCGGGGCCCT GGGCCCGGGA CTCCCAGGAA CCGGCCCCTG 
CTGCCCCTGC TGCTGCTTCT GCTCTTGCTA CCGCTGCCCG CCAGCGCCTG GTATAAGCAC 
GTGGCGAGTC CCCGCTATCA CACAGTGGGT CGTGCCTCCG GGCTGCTCAT GGGGCTGCGC 
CGCTCGCCCT ACCAGTGGCG CCGTGCCCTG GGCGGGGCTG CTGGACCCCT CTCCCGGCTC 
CCAGGACCGG TCGCCCGCGG CGCTCTCCTG CTTCCTTCCT CAGGGCAGGA GCTGTGGGAG 
GTACGAAGCA GGAGCTCACC TGCAGGGCTT CCCGTCCATG CACCCTGGAG TCCGCGGGAC 
CTGGAGGGAG TCCGCCAACC GGAGCAGTCG CTAAGCCTTC ACTCCTGGAT CTCAGAGGAG 
CCCGCTGCTA GAGCCTTCGG AGAGACGCTT CGTGCCCAGC CATGGTTCCT GCAGCAAGTC 
ATCTTTGCCG ATCCTGTCAG GCCCAAGAAC CGATGGCGCC CCCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npw ( NM_001099664.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001093134.1 Mus musculus neuropeptide W (Npw), mRNA.

Price & Availability↑

CloneID	OMu17775
Clone ID Related Accession (Same CDS sequence)	NM_001099664.1 , NM_001099664.2
Accession Version	NM_001099664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	neuropeptide W preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154566.2. On Jun 25, 2019 this sequence version replaced NM_001099664.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BY708553.1, AK131881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 12719537 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGCGTCTA ACAGAGAAGT GCGGGGCCCT GGGCCCGGGA CTCCCAGGAA CCGGCCCCTG 
CTGCCCCTGC TGCTGCTTCT GCTCTTGCTA CCGCTGCCCG CCAGCGCCTG GTATAAGCAC 
GTGGCGAGTC CCCGCTATCA CACAGTGGGT CGTGCCTCCG GGCTGCTCAT GGGGCTGCGC 
CGCTCGCCCT ACCAGTGGCG CCGTGCCCTG GGCGGGGCTG CTGGACCCCT CTCCCGGCTC 
CCAGGACCGG TCGCCCGCGG CGCTCTCCTG CTTCCTTCCT CAGGGCAGGA GCTGTGGGAG 
GTACGAAGCA GGAGCTCACC TGCAGGGCTT CCCGTCCATG CACCCTGGAG TCCGCGGGAC 
CTGGAGGGAG TCCGCCAACC GGAGCAGTCG CTAAGCCTTC ACTCCTGGAT CTCAGAGGAG 
CCCGCTGCTA GAGCCTTCGG AGAGACGCTT CGTGCCCAGC CATGGTTCCT GCAGCAAGTC 
ATCTTTGCCG ATCCTGTCAG GCCCAAGAAC CGATGGCGCC CCCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093134.1
CDS	128..658
Translation	LASNREVRGPGPGTPRNRPLLPLLLLLLLLPLPASAWYKHVASPRYHTVGRASGLLMGLRRSPYQWRRALGGAAGPLSRLPGPVARGALLLPSSGQELWEVRSRSSPAGLPVHAPWSPRDLEGVRQPEQSLSLHSWISEEPAARAFGETLRAQPWFLQQVIFADPVRPKNRWRPHA

Target ORF information:

RefSeq Version	NM_001099664.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuropeptide W (Npw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099664.2

ORF Insert Sequence:

CTGGCGTCTA ACAGAGAAGT GCGGGGCCCT GGGCCCGGGA CTCCCAGGAA CCGGCCCCTG 
CTGCCCCTGC TGCTGCTTCT GCTCTTGCTA CCGCTGCCCG CCAGCGCCTG GTATAAGCAC 
GTGGCGAGTC CCCGCTATCA CACAGTGGGT CGTGCCTCCG GGCTGCTCAT GGGGCTGCGC 
CGCTCGCCCT ACCAGTGGCG CCGTGCCCTG GGCGGGGCTG CTGGACCCCT CTCCCGGCTC 
CCAGGACCGG TCGCCCGCGG CGCTCTCCTG CTTCCTTCCT CAGGGCAGGA GCTGTGGGAG 
GTACGAAGCA GGAGCTCACC TGCAGGGCTT CCCGTCCATG CACCCTGGAG TCCGCGGGAC 
CTGGAGGGAG TCCGCCAACC GGAGCAGTCG CTAAGCCTTC ACTCCTGGAT CTCAGAGGAG 
CCCGCTGCTA GAGCCTTCGG AGAGACGCTT CGTGCCCAGC CATGGTTCCT GCAGCAAGTC 
ATCTTTGCCG ATCCTGTCAG GCCCAAGAAC CGATGGCGCC CCCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of brain-derived neurotrophic factor and nerve growth factor in the regulation of Neuropeptide W in?vitro and in?vivo.
Molecular and cellular endocrinology44771-78(2017 May)
Wang R,Yan F,Liao R,Wan P,Little PJ,Zheng W

Mesolimbic neuropeptide W coordinates stress responses under novel environments.
Proceedings of the National Academy of Sciences of the United States of America113(21)6023-8(2016 May)
Motoike T,Long JM,Tanaka H,Sinton CM,Skach A,Williams SC,Hammer RE,Sakurai T,Yanagisawa M

Neuropeptide W modulation of gastric vagal afferent mechanosensitivity: Impact of age and sex.
Peptides71141-8(2015 Sep)
Li H,Frisby CL,O'Donnell TA,Kentish SJ,Wittert GA,Page AJ

Transient expression of neuropeptide W in postnatal mouse hypothalamus--a putative regulator of energy homeostasis.
Neuroscience301323-37(2015 Aug)
Motoike T,Skach AG,Godwin JK,Sinton CM,Yamazaki M,Abe M,Natsume R,Sakimura K,Yanagisawa M

Gastric neuropeptide W is regulated by meal-related nutrients.
Peptides626-14(2014 Dec)
Li H,Feinle-Bisset C,Frisby C,Kentish S,Wittert GA,Page AJ

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Npw cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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