NPW cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPW
Entrez Gene ID	283869
Full Name	neuropeptide W
Synonyms	L8,L8C,PPL8,PPNPW
General protein information	Preferred Names neuropeptide W Names neuropeptide W hPPL8 prepro-neuropeptide W preproprotein L8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The product of this gene is processed into 23- and 30-amino acid neuropeptides that bind and activate two G-protein coupled receptors in the central nervous system. The neuropeptides have been shown to enhance cortisol secretion from adrenal cells through the adenylate cyclase/protein kinase A signaling cascade. The preproprotein is translated using a non-AUG initiation codon that is inferred from analyses of the mouse ortholog. [provided by RefSeq, Jul 2008].
Disorder MIM:	607997

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099456.2	NP_001092926.2	neuropeptide W preproprotein
NM_001099456.3	NP_001092926.2	neuropeptide W preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	NPW	NP_001092926.2
	NPW	XP_001082966.2
Rattus norvegicus (Norway rat)	Npw	NP_695206.1
Mus musculus (house mouse)	Npw	NP_001093134.1
Pan troglodytes (chimpanzee)	NPW	XP_003952809.1

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of CaV1.2 calcium channel by neuropeptide W regulates vascular myogenic tone via G protein-coupled receptor 7.
Ji L, Zhu H, Chen H, Fan W, Chen J, Chen J, Zhu G, Wang J
Journal of hypertension33(12)2431-42(2015 Dec)

Gastric neuropeptide W is regulated by meal-related nutrients.
Li H, Feinle-Bisset C, Frisby C, Kentish S, Wittert GA, Page AJ
Peptides626-14(2014 Dec)

Analgesic neuropeptide W suppresses seizures in the brain revealed by rational repositioning and peptide engineering.
Green BR, Smith M, White KL, White HS, Bulaj G
ACS chemical neuroscience2(1)51-6(2011 Jan)

Reviews in molecular biology and biotechnology: transmembrane signaling by G protein-coupled receptors.
Luttrell LM
Molecular biotechnology39(3)239-64(2008 Jul)

Dissociation of heterotrimeric g proteins in cells.
Lambert NA
Science signaling1(25)re5(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data highlight that NPW modulates the CaV1.2 currents via G protein-coupled receptor 7, and the presence of it influences arterial tone.
Title: Modulation of CaV1.2 calcium channel by neuropeptide W regulates vascular myogenic tone via G protein-coupled receptor 7.

these studies indicated an inconsistency between plasma and gastric NPW expression in response to nutrient ingestion, suggesting food induced gastric NPW expression may play a more important role locally
Title: Gastric neuropeptide W is regulated by meal-related nutrients.

This review of NPW suggests that study of effects of this peptide may reveal its role in regulating feeding and energy metabolism coupled to leptin levels in the brain.
Title: Analgesic neuropeptide W suppresses seizures in the brain revealed by rational repositioning and peptide engineering.

Intracerebroventricular administration of NPW23 and NPW30 to free-feeding rats suppressed dark phase and fasting-induced food intake. Possibly NPW functions as an endogenous catabolic signaling molecule in the brain.
Title: A role for neuropeptide W in the regulation of feeding behavior.

a non-AUG translation initiation site is used to express mouse prepro-Npw
Title: Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8.

The following NPW gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPW cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08911	NM_001099456.3 Latest version!	Homo sapiens neuropeptide W (NPW), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu08911	NM_001099456.2	Homo sapiens neuropeptide W (NPW), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPW ( NM_001099456.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092926.2 Homo sapiens neuropeptide W (NPW), mRNA.

Price & Availability↑

CloneID	OHu08911
Clone ID Related Accession (Same CDS sequence)	NM_001099456.2 , NM_001099456.3
Accession Version	NM_001099456.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	neuropeptide W preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK817957.1, AB084276.1 and BM985063.1. On or before Jul 24, 2007 this sequence version replaced XM_496111.3, XM_939689.2, NM_001099456.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM985063.1, SRR1163657.479299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 12719537, 12130646 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGCGTGGC GCCCAGGGGA GCGGGGGGCT CCCGCGAGCC GGCCGCGGCT GGCACTGCTG 
CTGCTTCTGC TCCTGCTGCC GCTGCCCTCC GGCGCGTGGT ACAAGCACGT GGCGAGTCCC 
CGCTACCACA CGGTGGGCCG CGCCGCTGGC CTGCTCATGG GGCTGCGTCG CTCACCCTAT 
CTGTGGCGCC GCGCGCTGCG CGCGGCCGCC GGGCCCCTGG CCAGGGACAC CCTCTCCCCC 
GAACCCGCAG CCCGCGAGGC TCCTCTCCTG CTGCCCTCGT GGGTTCAGGA GCTGTGGGAG 
ACGCGACGCA GGAGCTCCCA GGCAGGGATC CCCGTCCGTG CGCCCCGGAG CCCGCGCGCC 
CCAGAGCCTG CGCTGGAACC GGAGTCCCTG GACTTCAGCG GAGCTGGCCA GAGACTTCGG 
AGAGACGTCT CCCGCCCAGC GGTGGACCCC GCAGCAAACC GCCTTGGCCT GCCCTGCCTG 
GCCCCCGGAC CGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092926.2
CDS	383..880
Translation	LAWRPGERGAPASRPRLALLLLLLLLPLPSGAWYKHVASPRYHTVGRAAGLLMGLRRSPYLWRRALRAAAGPLARDTLSPEPAAREAPLLLPSWVQELWETRRRSSQAGIPVRAPRSPRAPEPALEPESLDFSGAGQRLRRDVSRPAVDPAANRLGLPCLAPGPF

Target ORF information:

RefSeq Version	NM_001099456.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide W (NPW), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099456.2

ORF Insert Sequence:

CTGGCGTGGC GCCCAGGGGA GCGGGGGGCT CCCGCGAGCC GGCCGCGGCT GGCACTGCTG 
CTGCTTCTGC TCCTGCTGCC GCTGCCCTCC GGCGCGTGGT ACAAGCACGT GGCGAGTCCC 
CGCTACCACA CGGTGGGCCG CGCCGCTGGC CTGCTCATGG GGCTGCGTCG CTCACCCTAT 
CTGTGGCGCC GCGCGCTGCG CGCGGCCGCC GGGCCCCTGG CCAGGGACAC CCTCTCCCCC 
GAACCCGCAG CCCGCGAGGC TCCTCTCCTG CTGCCCTCGT GGGTTCAGGA GCTGTGGGAG 
ACGCGACGCA GGAGCTCCCA GGCAGGGATC CCCGTCCGTG CGCCCCGGAG CCCGCGCGCC 
CCAGAGCCTG CGCTGGAACC GGAGTCCCTG GACTTCAGCG GAGCTGGCCA GAGACTTCGG 
AGAGACGTCT CCCGCCCAGC GGTGGACCCC GCAGCAAACC GCCTTGGCCT GCCCTGCCTG 
GCCCCCGGAC CGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPW ( NM_001099456.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092926.2 Homo sapiens neuropeptide W (NPW), mRNA.

Price & Availability↑

CloneID	OHu08911
Clone ID Related Accession (Same CDS sequence)	NM_001099456.2 , NM_001099456.3
Accession Version	NM_001099456.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	neuropeptide W preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB084276.1 and BM985063.1. On Nov 23, 2018 this sequence version replaced NM_001099456.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM985063.1, SRR1163657.479299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 12719537, 12130646 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGCGTGGC GCCCAGGGGA GCGGGGGGCT CCCGCGAGCC GGCCGCGGCT GGCACTGCTG 
CTGCTTCTGC TCCTGCTGCC GCTGCCCTCC GGCGCGTGGT ACAAGCACGT GGCGAGTCCC 
CGCTACCACA CGGTGGGCCG CGCCGCTGGC CTGCTCATGG GGCTGCGTCG CTCACCCTAT 
CTGTGGCGCC GCGCGCTGCG CGCGGCCGCC GGGCCCCTGG CCAGGGACAC CCTCTCCCCC 
GAACCCGCAG CCCGCGAGGC TCCTCTCCTG CTGCCCTCGT GGGTTCAGGA GCTGTGGGAG 
ACGCGACGCA GGAGCTCCCA GGCAGGGATC CCCGTCCGTG CGCCCCGGAG CCCGCGCGCC 
CCAGAGCCTG CGCTGGAACC GGAGTCCCTG GACTTCAGCG GAGCTGGCCA GAGACTTCGG 
AGAGACGTCT CCCGCCCAGC GGTGGACCCC GCAGCAAACC GCCTTGGCCT GCCCTGCCTG 
GCCCCCGGAC CGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092926.2
CDS	118..615
Translation	LAWRPGERGAPASRPRLALLLLLLLLPLPSGAWYKHVASPRYHTVGRAAGLLMGLRRSPYLWRRALRAAAGPLARDTLSPEPAAREAPLLLPSWVQELWETRRRSSQAGIPVRAPRSPRAPEPALEPESLDFSGAGQRLRRDVSRPAVDPAANRLGLPCLAPGPF

Target ORF information:

RefSeq Version	NM_001099456.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide W (NPW), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099456.3

ORF Insert Sequence:

CTGGCGTGGC GCCCAGGGGA GCGGGGGGCT CCCGCGAGCC GGCCGCGGCT GGCACTGCTG 
CTGCTTCTGC TCCTGCTGCC GCTGCCCTCC GGCGCGTGGT ACAAGCACGT GGCGAGTCCC 
CGCTACCACA CGGTGGGCCG CGCCGCTGGC CTGCTCATGG GGCTGCGTCG CTCACCCTAT 
CTGTGGCGCC GCGCGCTGCG CGCGGCCGCC GGGCCCCTGG CCAGGGACAC CCTCTCCCCC 
GAACCCGCAG CCCGCGAGGC TCCTCTCCTG CTGCCCTCGT GGGTTCAGGA GCTGTGGGAG 
ACGCGACGCA GGAGCTCCCA GGCAGGGATC CCCGTCCGTG CGCCCCGGAG CCCGCGCGCC 
CCAGAGCCTG CGCTGGAACC GGAGTCCCTG GACTTCAGCG GAGCTGGCCA GAGACTTCGG 
AGAGACGTCT CCCGCCCAGC GGTGGACCCC GCAGCAAACC GCCTTGGCCT GCCCTGCCTG 
GCCCCCGGAC CGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of CaV1.2 calcium channel by neuropeptide W regulates vascular myogenic tone via G protein-coupled receptor 7.
Journal of hypertension33(12)2431-42(2015 Dec)
Ji L,Zhu H,Chen H,Fan W,Chen J,Chen J,Zhu G,Wang J

Gastric neuropeptide W is regulated by meal-related nutrients.
Peptides626-14(2014 Dec)
Li H,Feinle-Bisset C,Frisby C,Kentish S,Wittert GA,Page AJ

Analgesic neuropeptide W suppresses seizures in the brain revealed by rational repositioning and peptide engineering.
ACS chemical neuroscience2(1)51-6(2011 Jan)
Green BR,Smith M,White KL,White HS,Bulaj G

Reviews in molecular biology and biotechnology: transmembrane signaling by G protein-coupled receptors.
Molecular biotechnology39(3)239-64(2008 Jul)
Luttrell LM

Dissociation of heterotrimeric g proteins in cells.
Science signaling1(25)re5(2008 Jun)
Lambert NA

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NPW cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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