Hfm1 cDNA ORF clone, Mus musculus(house mouse)

The following Hfm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hfm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu05172 NM_001252516.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu81399 XM_017320933.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu06964 NM_177873.3
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OMu35526 XM_006535016.4
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu74856 XM_030254593.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu74858 XM_030254596.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81398 XM_030254594.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu81398 XM_017320932.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu88942 XM_030254590.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu88943 XM_030254591.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu88943 XM_030254592.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu88944 XM_030254595.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu88945 XM_030254600.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu88945 XM_030254599.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu88945 XM_030254597.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu88945 XM_030254598.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu74858 XM_011249488.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu35526 XM_011249484.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35526 XM_006535015.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu74855 XM_011249485.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu74856 XM_011249486.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu74857 XM_011249487.1
Latest version!
Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OMu35526 XM_006535016.3 Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu05172
    Clone ID Related Accession (Same CDS sequence) NM_001252516.1
    Accession Version NM_001252516.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-24
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039266.1. Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in an isoform (2) with shorter and distinct N- and C-termini and a translation initiation site at a downstream start codon, compared to variant 1. ##Evidence-Data-START## Transcript exon combination :: AK039266.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849384, SAMN01164131 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GCCCAGTGAA ACTTACCCAA ACGAAAATAA GCAAAGAGAA TGCATGGACG
    TGTAGCAATA GTAAGCAGAA ACGTCAGTAT TCTACAAACA AATTCAAAGC AAATGATGCT
    TTTTCTGCTT CTGGAATTGG AAAAGACATA TTCAAAGCAC CATCATTTCC TGCTGCATCT
    CAGCCTCATG ATATTCAAGG AATAACCCCA AATGGCTTAG GTTCTTTGAA AGCTGTCACG
    GAAATCCCGG CAAAATTTAG AAATATATTC AAAGAATTCC CTTACTTCAA CTATATACAA
    TCCAAAGCCT TTGATGATCT TCTTTACACA GATAGGAATT TTGTGATTTG TGCCCCCACT
    GGTTCTGGGA AAACTGTAGT GTTTGAGCTA GCGATAACAA GATTGTTAAT GGAAGTACCA
    TTGCCATGGC TAAACATGAA GATTGTTTAC AGAGAAGTGG GACAGTGTGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239445.1
    CDS310..1203
    Translation

    Target ORF information:

    RefSeq Version NM_001252516.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GCCCAGTGAA ACTTACCCAA ACGAAAATAA GCAAAGAGAA TGCATGGACG
    TGTAGCAATA GTAAGCAGAA ACGTCAGTAT TCTACAAACA AATTCAAAGC AAATGATGCT
    TTTTCTGCTT CTGGAATTGG AAAAGACATA TTCAAAGCAC CATCATTTCC TGCTGCATCT
    CAGCCTCATG ATATTCAAGG AATAACCCCA AATGGCTTAG GTTCTTTGAA AGCTGTCACG
    GAAATCCCGG CAAAATTTAG AAATATATTC AAAGAATTCC CTTACTTCAA CTATATACAA
    TCCAAAGCCT TTGATGATCT TCTTTACACA GATAGGAATT TTGTGATTTG TGCCCCCACT
    GGTTCTGGGA AAACTGTAGT GTTTGAGCTA GCGATAACAA GATTGTTAAT GGAAGTACCA
    TTGCCATGGC TAAACATGAA GATTGTTTAC AGAGAAGTGG GACAGTGTGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81399
    Clone ID Related Accession (Same CDS sequence) XM_017320933.1
    Accession Version XM_017320933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGG GATTCTGTCT
    TGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176422.1
    CDS211..2556
    Translation

    Target ORF information:

    RefSeq Version XM_017320933.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGG GATTCTGTCT
    TGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu06964
    Clone ID Related Accession (Same CDS sequence) NM_177873.3
    Accession Version NM_177873.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC137947.12. On Nov 16, 2011 this sequence version replaced NM_177873.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01164139 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGAAGT TCTGGGTGTG
    AACTTAGAGC TGGGAAATGA AGTCTGGGAT GACTTTGATG ATGAGAGCTT AATAGAAGTT
    ATGAGCCTTT CAGCTGATGC TGAGAAGATG GCTGCATCAG GATTTGGAGA CACTCGTGAT
    TCAAGTCTCG GCGGAAGTAA GCTACCCTTC CAAAAGTCAA GCAGCAGATT CCAAAGAGAC
    AACTCAAACA GTTTTGCTTC GTCGCCTGGG AAGCCAGATG CTTATTTGCG AGATTGTTCC
    AGGTCCAGCT TCGGGCTGTC CTCTGTGGCA GAGATCCCTC AGCGGGCTGA GAATGCAAGT
    CTTGCCAATT TACAAGAGAG GAGACCGCTG ACTCTCTCAC CAGTGATTGA GAGGATGTGC
    TTTGCCCACT CTAAGAAAAC ACCACAGTCT CCCAAGTTTA AGGAAGTGGA TATTTTTATT
    GGAAACAGTG GCTCTAAAAA GGAAATTGAT CTTAGTAAGT ATTATCCTGA TGATGCAGCT
    GAAGAAATGA AGGCTCTTCT GGGAATATTT AATGGCATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808541.2
    CDS252..4556
    Translation

    Target ORF information:

    RefSeq Version NM_177873.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177873.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGAAGT TCTGGGTGTG
    AACTTAGAGC TGGGAAATGA AGTCTGGGAT GACTTTGATG ATGAGAGCTT AATAGAAGTT
    ATGAGCCTTT CAGCTGATGC TGAGAAGATG GCTGCATCAG GATTTGGAGA CACTCGTGAT
    TCAAGTCTCG GCGGAAGTAA GCTACCCTTC CAAAAGTCAA GCAGCAGATT CCAAAGAGAC
    AACTCAAACA GTTTTGCTTC GTCGCCTGGG AAGCCAGATG CTTATTTGCG AGATTGTTCC
    AGGTCCAGCT TCGGGCTGTC CTCTGTGGCA GAGATCCCTC AGCGGGCTGA GAATGCAAGT
    CTTGCCAATT TACAAGAGAG GAGACCGCTG ACTCTCTCAC CAGTGATTGA GAGGATGTGC
    TTTGCCCACT CTAAGAAAAC ACCACAGTCT CCCAAGTTTA AGGAAGTGGA TATTTTTATT
    GGAAACAGTG GCTCTAAAAA GGAAATTGAT CTTAGTAAGT ATTATCCTGA TGATGCAGCT
    GAAGAAATGA AGGCTCTTCT GGGAATATTT AATGGCATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35526
    Clone ID Related Accession (Same CDS sequence) XM_006535016.3 , XM_011249484.1 , XM_006535015.1 , XM_006535016.4
    Accession Version XM_006535016.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535016.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006535079.1
    CDS225..4655
    Translation

    Target ORF information:

    RefSeq Version XM_006535016.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006535016.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74856
    Clone ID Related Accession (Same CDS sequence) XM_030254593.1 , XM_011249486.1
    Accession Version XM_030254593.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAACTGA AAAGGAAAGG AAAGATGCCA AGAATGTTAA CATCAAACTT GAAGATCATT 
    AATGAAGACA CAAATTGTAT TTCACCAACA CAAAAAATCC ATTTTTCCTA TAATGTACAT
    GAACAGGATT ACCTAAATTT AGGAGGATCA AATAACAATG ACATGTCCCA TGTAGCTGGG
    AAGCTGATGT ATGGTTCTTC TCAGAAATAT AAAAATCACA TGGGTGCTAA GAGTCCATCT
    GCAAGGAGTA GTCCTGGTGA TACAAAATTA CATGATGTGG CAGAAGACAG ACAGGGCACA
    TCAGCATTCA AAAAAAGACT GTCTAGAACA TGTGACAGTG AACATGACTG TGACTATGCT
    GATGGAAGTT TAAACTTGAG TTCTCATATC AGCCCAGTGA AACTTACCCA AACGAAAATA
    AGCAAAGAGA ATGCATGGAC GTGTAGCAAT AGTAAGCAGA AACGTCAGTA TTCTACAAAC
    AAATTCAAAG CAAATGATGC TTTTTCTGCT TCTGGAATTG GAAAAGACAT ATTCAAAGCA
    CCATCATTTC CTGCTGCATC TCAGCCTCAT GATATTCAAG GAATAACCCC AAATGGCTTA
    GGTTCTTTGA AAGCTGTCAC GGAAATCCCG GCAAAATTTA GAAATATATT CAAAGAATTC
    CCTTACTTCA ACTATATACA ATCCAAAGCC TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCCCCCAC TGGTTCTGGG AAAACTGTAG TGTTTGAGCT AGCGATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG CTAAACATGA AGATTGTTTA CATGGCACCA
    ATAAAAGCCC TTTGTAGTCA GCGGTTTGAT GACTGGAAAG AAAAGTTTGG ACCAGTAGGC
    TTGAATTGTA AAGAACTTAC TGGTGATACA GTAATGGATG ACCTGTTTGA GATTCAGCAT
    GCAAATATAA TTATAACAAC TCCAGAGAAG TGGGACAGTG TGACTAGGAA GTGGAGAGAC
    AACTCTTTTA TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTGCATGT TATTAAAGAT
    GAAAATCGTG GTCCAACTCT TGAAGTTGTA GTTAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTAGGGATT TAGAAAGTGC CAGCCCTGTT CCAGTGAGAT TTGTAGCTGT GTCTGCAACA
    ATTCCCAATG CTGAGGATAT CGCAGAATGG CTTTCAGATG GTGAGAGACC TGCTGTATGT
    CTCAAAATGG ATGAGAGCCA TAGACCTGTG AAACTTCAGA AAGTAGTCCT GGGTTTTCCC
    TGTAGTAGTA GCCAAACTGA ATTTAAGTTT GATTTGGCCC TCAACTATAA AGTGTACAGT
    GTTATTCGAA CATACTCCGA TCAGAAGCCC ACACTTGTGT TTTGTTCAAC AAGGAAAGGT
    GTGCAGCAGG CTGCTTCTGT TCTTGTGAAG GATGCTAAGT TTATCATCTC TGTGGAGCAG
    AAACTACGGT TGCAGAAGTC TGCATATTCT ATAAGGGATT CAAAGCTGAA AGATACCTTG
    GTATATGGTG TTGGTTACCA TCATGCTGGA ATGGAGCTGT CAGATAGAAA ATTGGTTGAA
    GGATTGTTTA CCTCTGGAGA TCTACCAGTT CTTTTCACTA CCAGTACTCT AGCTATGGGC
    ATGAACATGC CGGCTCACCT GGTTGTTATA AAGTCTACCA TGCACTACAG TGGAGGTGTG
    TTTGAAGAGT ACAGCGAGAC CGACATCCTG CAGATGATTG GGAGAGCTGG CAGACCCCAG
    TTTGACACTA CAGCTACTGC AGTCATTATG ACTCGACTAA GTACAAGAGA GAAGTACGTG
    CAGATGCTAG CTTGTAACGA CACTGTAGAG AGCAGTTTGC ACAGACATCT TATTGAACAT
    TTAAATGCAG AAATAGTGCT GCATACCATC ACAGACGTGA ACATTGCTCT GGACTGGATC
    CGATCAACAA TGCTGTATAT CAGAGCTCTG AAAAACCCAT CTCACTATGG TTTTTCATCT
    GGATTGAACA AAGATGGGAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGAAA
    GATTTATCAT CACTAGACCT GATAAAGATG GATGAAGATG TTAATTTTAA ACCAACAGAG
    GCAGGAAGAC TAATGGCTTG GTATTATATT ACATTTGAGA CAGTCAAGAA ATTTTGTGCA
    ATCAGTGGAA AAGAAACTTT ATTGGATTTA ATTTCAATGA TATCGAGCTG CAATGAGTTT
    CTAGATGTGC AGCTGAGGAT AAGTGAAAAG AGAATACTGA ACACCTTAAA CAAAGACCCA
    AACCGGATAA CTATCAGATT TCCAATGGCA GAAAGAATTA AAACAAGAGA AATGAAAGTA
    AACTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCTATAC AAGATTTTGC TTTGACACAA
    GATACAGTGA AGATTTTCAG AAATGGCTCA CGAATTGCAA GATGGTTGTC AGATTTTGTG
    GCTGCTCAGG AAAAGAAGTT TGCTGTGCTT TTAAACAGTG TGATTTTAAC TAAATGTTTT
    AAGTGTAAAC TCTGGGAAAA CTCTAAACAT GTATCAAAAC AGCTGGATAA AATTGGCATA
    TCATTGTCAA ATACCATGGT AAATGCAGGC TTAACTTCTT TTAAAAAAAT AGAAGAAGCA
    AATGCAAGAG AACTTGAACT GATTTTAAAT AGACACCCAC CATTTGGGAC CCAGATAAAA
    GAAGCTGTGG CGCATCTGCC GAAGTATGAG CTTGAGGTGG AGCAGATTGC AAGATACAGT
    GATATCAAGG CAGAAATACT GGTGACCATT ATATTAAGAA ACTTTGAGCA GCTGCAAACT
    AAAAGAACAG CACCAGACTT CCACTATGCC ACCTTAATCA TAGGTGATGC AGATAACCAA
    GTCGTTTTTA AGCACAAAAT TATGGATTCT GTCTTGTTAA AAAGTGGAAA TTGGGTTAAA
    AAAATTGATG TGAAGAGAGC TCTTATATCA GAAGATCTCA GCATAAATCT AATTAGTTCT
    GATTATGTTG GTCTTGATAT TCACCAGAAA TTTACAGTCT TTTATTTCGG ACCCAGGAAG
    TTTGTAAATG AAACAGCTAT GGAAAGAAGC TCAGAAACAG ATATTTCTCA TTCTGACTAC
    TCAGGTAGAG CCACTGCAAC AGGATCCAGT AAAGGAATGG CTACCTGCAA AAAACCTGGG
    AACAGAGAAT GCCATCATCA CTGTAAAAAT AAACACGCAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAAAAGCC GGAAGTTAAA GAGTCAGCAA TGTCTTCATA TCTGTCTGAC
    TTAAAAAGCA GAGATGCTGT TTCTTCTCTT CCTCTAGCCA AGCGACTCAA GATACAGATG
    AACAAATCTC AAAATGTGGA CCTTAAAGAG TTTGGTTTTA CTCCAAGGCC TTCCCTTTCC
    AGTATATCAA GGTCAGAATA TTTAAATACC CCTGAATTGT CAATATTAGA ACAGAGGAAT
    CAGCATGAAA TCTATGGAAA AGTTCAACAG GGACCATCTG AATATCGTGA CAAAGATTTT
    CCTAGATGGG ACTATTGTAC AACTTCAAGT GAAAAGACCC CTCAAACGGT GATGTTCAGC
    AGGCATGCTA ATATACCTGG AAACCACAGA GTTGCAGATG AAGACAAAAG CAAGTCTTCA
    GAAGTTCTGG GTGTGAACTT AGAGCTGGGA AATGAAGTCT GGGATGACTT TGATGATGAG
    AGCTTAATAG AAGTTATGAG CCTTTCAGCT GATGCTGAGA AGATGGCTGC ATCAGGATTT
    GGAGACACTC GTGATTCAAG TCTCGGCGGA AGTAAGCTAC CCTTCCAAAA GTCAAGCAGC
    AGATTCCAAA GAGACAACTC AAACAGTTTT GCTTCGTCGC CTGGGAAGCC AGATGCTTAT
    TTGCGAGATT GTTCCAGGTC CAGCTTCGGG CTGTCCTCTG TGGCAGAGAT CCCTCAGCGG
    GCTGAGAATG CAAGTCTTGC CAATTTACAA GAGAGGAGAC CGCTGACTCT CTCACCAGTG
    ATTGAGAGGA TGTGCTTTGC CCACTCTAAG AAAACACCAC AGTCTCCCAA GTTTAAGGAA
    GTGGATATTT TTATTGGAAA CAGTGGCTCT AAAAAGGAAA TTGATCTTAG TAAGTATTAT
    CCTGATGATG CAGCTGAAGA AATGAAGGCT CTTCTGGGAA TATTTAATGG CATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110453.1
    CDS269..4528
    Translation

    Target ORF information:

    RefSeq Version XM_030254593.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAACTGA AAAGGAAAGG AAAGATGCCA AGAATGTTAA CATCAAACTT GAAGATCATT 
    AATGAAGACA CAAATTGTAT TTCACCAACA CAAAAAATCC ATTTTTCCTA TAATGTACAT
    GAACAGGATT ACCTAAATTT AGGAGGATCA AATAACAATG ACATGTCCCA TGTAGCTGGG
    AAGCTGATGT ATGGTTCTTC TCAGAAATAT AAAAATCACA TGGGTGCTAA GAGTCCATCT
    GCAAGGAGTA GTCCTGGTGA TACAAAATTA CATGATGTGG CAGAAGACAG ACAGGGCACA
    TCAGCATTCA AAAAAAGACT GTCTAGAACA TGTGACAGTG AACATGACTG TGACTATGCT
    GATGGAAGTT TAAACTTGAG TTCTCATATC AGCCCAGTGA AACTTACCCA AACGAAAATA
    AGCAAAGAGA ATGCATGGAC GTGTAGCAAT AGTAAGCAGA AACGTCAGTA TTCTACAAAC
    AAATTCAAAG CAAATGATGC TTTTTCTGCT TCTGGAATTG GAAAAGACAT ATTCAAAGCA
    CCATCATTTC CTGCTGCATC TCAGCCTCAT GATATTCAAG GAATAACCCC AAATGGCTTA
    GGTTCTTTGA AAGCTGTCAC GGAAATCCCG GCAAAATTTA GAAATATATT CAAAGAATTC
    CCTTACTTCA ACTATATACA ATCCAAAGCC TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCCCCCAC TGGTTCTGGG AAAACTGTAG TGTTTGAGCT AGCGATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG CTAAACATGA AGATTGTTTA CATGGCACCA
    ATAAAAGCCC TTTGTAGTCA GCGGTTTGAT GACTGGAAAG AAAAGTTTGG ACCAGTAGGC
    TTGAATTGTA AAGAACTTAC TGGTGATACA GTAATGGATG ACCTGTTTGA GATTCAGCAT
    GCAAATATAA TTATAACAAC TCCAGAGAAG TGGGACAGTG TGACTAGGAA GTGGAGAGAC
    AACTCTTTTA TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTGCATGT TATTAAAGAT
    GAAAATCGTG GTCCAACTCT TGAAGTTGTA GTTAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTAGGGATT TAGAAAGTGC CAGCCCTGTT CCAGTGAGAT TTGTAGCTGT GTCTGCAACA
    ATTCCCAATG CTGAGGATAT CGCAGAATGG CTTTCAGATG GTGAGAGACC TGCTGTATGT
    CTCAAAATGG ATGAGAGCCA TAGACCTGTG AAACTTCAGA AAGTAGTCCT GGGTTTTCCC
    TGTAGTAGTA GCCAAACTGA ATTTAAGTTT GATTTGGCCC TCAACTATAA AGTGTACAGT
    GTTATTCGAA CATACTCCGA TCAGAAGCCC ACACTTGTGT TTTGTTCAAC AAGGAAAGGT
    GTGCAGCAGG CTGCTTCTGT TCTTGTGAAG GATGCTAAGT TTATCATCTC TGTGGAGCAG
    AAACTACGGT TGCAGAAGTC TGCATATTCT ATAAGGGATT CAAAGCTGAA AGATACCTTG
    GTATATGGTG TTGGTTACCA TCATGCTGGA ATGGAGCTGT CAGATAGAAA ATTGGTTGAA
    GGATTGTTTA CCTCTGGAGA TCTACCAGTT CTTTTCACTA CCAGTACTCT AGCTATGGGC
    ATGAACATGC CGGCTCACCT GGTTGTTATA AAGTCTACCA TGCACTACAG TGGAGGTGTG
    TTTGAAGAGT ACAGCGAGAC CGACATCCTG CAGATGATTG GGAGAGCTGG CAGACCCCAG
    TTTGACACTA CAGCTACTGC AGTCATTATG ACTCGACTAA GTACAAGAGA GAAGTACGTG
    CAGATGCTAG CTTGTAACGA CACTGTAGAG AGCAGTTTGC ACAGACATCT TATTGAACAT
    TTAAATGCAG AAATAGTGCT GCATACCATC ACAGACGTGA ACATTGCTCT GGACTGGATC
    CGATCAACAA TGCTGTATAT CAGAGCTCTG AAAAACCCAT CTCACTATGG TTTTTCATCT
    GGATTGAACA AAGATGGGAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGAAA
    GATTTATCAT CACTAGACCT GATAAAGATG GATGAAGATG TTAATTTTAA ACCAACAGAG
    GCAGGAAGAC TAATGGCTTG GTATTATATT ACATTTGAGA CAGTCAAGAA ATTTTGTGCA
    ATCAGTGGAA AAGAAACTTT ATTGGATTTA ATTTCAATGA TATCGAGCTG CAATGAGTTT
    CTAGATGTGC AGCTGAGGAT AAGTGAAAAG AGAATACTGA ACACCTTAAA CAAAGACCCA
    AACCGGATAA CTATCAGATT TCCAATGGCA GAAAGAATTA AAACAAGAGA AATGAAAGTA
    AACTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCTATAC AAGATTTTGC TTTGACACAA
    GATACAGTGA AGATTTTCAG AAATGGCTCA CGAATTGCAA GATGGTTGTC AGATTTTGTG
    GCTGCTCAGG AAAAGAAGTT TGCTGTGCTT TTAAACAGTG TGATTTTAAC TAAATGTTTT
    AAGTGTAAAC TCTGGGAAAA CTCTAAACAT GTATCAAAAC AGCTGGATAA AATTGGCATA
    TCATTGTCAA ATACCATGGT AAATGCAGGC TTAACTTCTT TTAAAAAAAT AGAAGAAGCA
    AATGCAAGAG AACTTGAACT GATTTTAAAT AGACACCCAC CATTTGGGAC CCAGATAAAA
    GAAGCTGTGG CGCATCTGCC GAAGTATGAG CTTGAGGTGG AGCAGATTGC AAGATACAGT
    GATATCAAGG CAGAAATACT GGTGACCATT ATATTAAGAA ACTTTGAGCA GCTGCAAACT
    AAAAGAACAG CACCAGACTT CCACTATGCC ACCTTAATCA TAGGTGATGC AGATAACCAA
    GTCGTTTTTA AGCACAAAAT TATGGATTCT GTCTTGTTAA AAAGTGGAAA TTGGGTTAAA
    AAAATTGATG TGAAGAGAGC TCTTATATCA GAAGATCTCA GCATAAATCT AATTAGTTCT
    GATTATGTTG GTCTTGATAT TCACCAGAAA TTTACAGTCT TTTATTTCGG ACCCAGGAAG
    TTTGTAAATG AAACAGCTAT GGAAAGAAGC TCAGAAACAG ATATTTCTCA TTCTGACTAC
    TCAGGTAGAG CCACTGCAAC AGGATCCAGT AAAGGAATGG CTACCTGCAA AAAACCTGGG
    AACAGAGAAT GCCATCATCA CTGTAAAAAT AAACACGCAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAAAAGCC GGAAGTTAAA GAGTCAGCAA TGTCTTCATA TCTGTCTGAC
    TTAAAAAGCA GAGATGCTGT TTCTTCTCTT CCTCTAGCCA AGCGACTCAA GATACAGATG
    AACAAATCTC AAAATGTGGA CCTTAAAGAG TTTGGTTTTA CTCCAAGGCC TTCCCTTTCC
    AGTATATCAA GGTCAGAATA TTTAAATACC CCTGAATTGT CAATATTAGA ACAGAGGAAT
    CAGCATGAAA TCTATGGAAA AGTTCAACAG GGACCATCTG AATATCGTGA CAAAGATTTT
    CCTAGATGGG ACTATTGTAC AACTTCAAGT GAAAAGACCC CTCAAACGGT GATGTTCAGC
    AGGCATGCTA ATATACCTGG AAACCACAGA GTTGCAGATG AAGACAAAAG CAAGTCTTCA
    GAAGTTCTGG GTGTGAACTT AGAGCTGGGA AATGAAGTCT GGGATGACTT TGATGATGAG
    AGCTTAATAG AAGTTATGAG CCTTTCAGCT GATGCTGAGA AGATGGCTGC ATCAGGATTT
    GGAGACACTC GTGATTCAAG TCTCGGCGGA AGTAAGCTAC CCTTCCAAAA GTCAAGCAGC
    AGATTCCAAA GAGACAACTC AAACAGTTTT GCTTCGTCGC CTGGGAAGCC AGATGCTTAT
    TTGCGAGATT GTTCCAGGTC CAGCTTCGGG CTGTCCTCTG TGGCAGAGAT CCCTCAGCGG
    GCTGAGAATG CAAGTCTTGC CAATTTACAA GAGAGGAGAC CGCTGACTCT CTCACCAGTG
    ATTGAGAGGA TGTGCTTTGC CCACTCTAAG AAAACACCAC AGTCTCCCAA GTTTAAGGAA
    GTGGATATTT TTATTGGAAA CAGTGGCTCT AAAAAGGAAA TTGATCTTAG TAAGTATTAT
    CCTGATGATG CAGCTGAAGA AATGAAGGCT CTTCTGGGAA TATTTAATGG CATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74858
    Clone ID Related Accession (Same CDS sequence) XM_011249488.1 , XM_030254596.1
    Accession Version XM_030254596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC 
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110456.1
    CDS372..3842
    Translation

    Target ORF information:

    RefSeq Version XM_030254596.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC 
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81398
    Clone ID Related Accession (Same CDS sequence) XM_030254594.1 , XM_017320932.1
    Accession Version XM_030254594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAGAA TGTTAACATC AAACTTGAAG ATCATTAATG AAGACACAAA TTGTATTTCA 
    CCAACACAAA AAATCCATTT TTCCTATAAT GTACATGAAC AGGATTACCT AAATTTAGGA
    GGATCAAATA ACAATGACAT GTCCCATGTA GCTGGGAAGC TGATGTATGG TTCTTCTCAG
    AAATATAAAA ATCACATGGG TGCTAAGAGT CCATCTGCAA GGAGTAGTCC TGGTGATACA
    AAATTACATG ATGTGGCAGA AGACAGACAG GGCACATCAG CATTCAAAAA AAGACTGTCT
    AGAACATGTG ACAGTGAACA TGACTGTGAC TATGCTGATG GAAGTTTAAA CTTGAGTTCT
    CATATCAGCC CAGTGAAACT TACCCAAACG AAAATAAGCA AAGAGAATGC ATGGACGTGT
    AGCAATAGTA AGCAGAAACG TCAGTATTCT ACAAACAAAT TCAAAGCAAA TGATGCTTTT
    TCTGCTTCTG GAATTGGAAA AGACATATTC AAAGCACCAT CATTTCCTGC TGCATCTCAG
    CCTCATGATA TTCAAGGAAT AACCCCAAAT GGCTTAGGTT CTTTGAAAGC TGTCACGGAA
    ATCCCGGCAA AATTTAGAAA TATATTCAAA GAATTCCCTT ACTTCAACTA TATACAATCC
    AAAGCCTTTG ATGATCTTCT TTACACAGAT AGGAATTTTG TGATTTGTGC CCCCACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAGCTAGCG ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGCTAA ACATGAAGAT TGTTTACATG GCACCAATAA AAGCCCTTTG TAGTCAGCGG
    TTTGATGACT GGAAAGAAAA GTTTGGACCA GTAGGCTTGA ATTGTAAAGA ACTTACTGGT
    GATACAGTAA TGGATGACCT GTTTGAGATT CAGCATGCAA ATATAATTAT AACAACTCCA
    GAGAAGTGGG ACAGTGTGAC TAGGAAGTGG AGAGACAACT CTTTTATCCA ACTGGTTCGA
    CTGTTTCTCA TTGATGAGGT GCATGTTATT AAAGATGAAA ATCGTGGTCC AACTCTTGAA
    GTTGTAGTTA GCAGAATGAA AACTGTACAG TCTTTATCTA GGGATTTAGA AAGTGCCAGC
    CCTGTTCCAG TGAGATTTGT AGCTGTGTCT GCAACAATTC CCAATGCTGA GGATATCGCA
    GAATGGCTTT CAGATGGTGA GAGACCTGCT GTATGTCTCA AAATGGATGA GAGCCATAGA
    CCTGTGAAAC TTCAGAAAGT AGTCCTGGGT TTTCCCTGTA GTAGTAGCCA AACTGAATTT
    AAGTTTGATT TGGCCCTCAA CTATAAAGTG TACAGTGTTA TTCGAACATA CTCCGATCAG
    AAGCCCACAC TTGTGTTTTG TTCAACAAGG AAAGGTGTGC AGCAGGCTGC TTCTGTTCTT
    GTGAAGGATG CTAAGTTTAT CATCTCTGTG GAGCAGAAAC TACGGTTGCA GAAGTCTGCA
    TATTCTATAA GGGATTCAAA GCTGAAAGAT ACCTTGGTAT ATGGTGTTGG TTACCATCAT
    GCTGGAATGG AGCTGTCAGA TAGAAAATTG GTTGAAGGAT TGTTTACCTC TGGAGATCTA
    CCAGTTCTTT TCACTACCAG TACTCTAGCT ATGGGCATGA ACATGCCGGC TCACCTGGTT
    GTTATAAAGT CTACCATGCA CTACAGTGGA GGTGTGTTTG AAGAGTACAG CGAGACCGAC
    ATCCTGCAGA TGATTGGGAG AGCTGGCAGA CCCCAGTTTG ACACTACAGC TACTGCAGTC
    ATTATGACTC GACTAAGTAC AAGAGAGAAG TACGTGCAGA TGCTAGCTTG TAACGACACT
    GTAGAGAGCA GTTTGCACAG ACATCTTATT GAACATTTAA ATGCAGAAAT AGTGCTGCAT
    ACCATCACAG ACGTGAACAT TGCTCTGGAC TGGATCCGAT CAACAATGCT GTATATCAGA
    GCTCTGAAAA ACCCATCTCA CTATGGTTTT TCATCTGGAT TGAACAAAGA TGGGATTGAA
    GCAAAATTAC AAGAATTATG TTTGAAGAAT CTGAAAGATT TATCATCACT AGACCTGATA
    AAGATGGATG AAGATGTTAA TTTTAAACCA ACAGAGGCAG GAAGACTAAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT CAAGAAATTT TGTGCAATCA GTGGAAAAGA AACTTTATTG
    GATTTAATTT CAATGATATC GAGCTGCAAT GAGTTTCTAG ATGTGCAGCT GAGGATAAGT
    GAAAAGAGAA TACTGAACAC CTTAAACAAA GACCCAAACC GGATAACTAT CAGATTTCCA
    ATGGCAGAAA GAATTAAAAC AAGAGAAATG AAAGTAAACT GTCTTATTCA GGCTCAGCTA
    GGATGCATTC CTATACAAGA TTTTGCTTTG ACACAAGATA CAGTGAAGAT TTTCAGAAAT
    GGCTCACGAA TTGCAAGATG GTTGTCAGAT TTTGTGGCTG CTCAGGAAAA GAAGTTTGCT
    GTGCTTTTAA ACAGTGTGAT TTTAACTAAA TGTTTTAAGT GTAAACTCTG GGAAAACTCT
    AAACATGTAT CAAAACAGCT GGATAAAATT GGCATATCAT TGTCAAATAC CATGGTAAAT
    GCAGGCTTAA CTTCTTTTAA AAAAATAGAA GAAGCAAATG CAAGAGAACT TGAACTGATT
    TTAAATAGAC ACCCACCATT TGGGACCCAG ATAAAAGAAG CTGTGGCGCA TCTGCCGAAG
    TATGAGCTTG AGGTGGAGCA GATTGCAAGA TACAGTGATA TCAAGGCAGA AATACTGGTG
    ACCATTATAT TAAGAAACTT TGAGCAGCTG CAAACTAAAA GAACAGCACC AGACTTCCAC
    TATGCCACCT TAATCATAGG TGATGCAGAT AACCAAGTCG TTTTTAAGCA CAAAATTATG
    GATTCTGTCT TGTTAAAAAG TGGAAATTGG GTTAAAAAAA TTGATGTGAA GAGAGCTCTT
    ATATCAGAAG ATCTCAGCAT AAATCTAATT AGTTCTGATT ATGTTGGTCT TGATATTCAC
    CAGAAATTTA CAGTCTTTTA TTTCGGACCC AGGAAGTTTG TAAATGAAAC AGCTATGGAA
    AGAAGCTCAG AAACAGATAT TTCTCATTCT GACTACTCAG GTAGAGCCAC TGCAACAGGA
    TCCAGTAAAG GAATGGCTAC CTGCAAAAAA CCTGGGAACA GAGAATGCCA TCATCACTGT
    AAAAATAAAC ACGCATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA AAAGCCGGAA
    GTTAAAGAGT CAGCAATGTC TTCATATCTG TCTGACTTAA AAAGCAGAGA TGCTGTTTCT
    TCTCTTCCTC TAGCCAAGCG ACTCAAGATA CAGATGAACA AATCTCAAAA TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAGGCCTTCC CTTTCCAGTA TATCAAGGTC AGAATATTTA
    AATACCCCTG AATTGTCAAT ATTAGAACAG AGGAATCAGC ATGAAATCTA TGGAAAAGTT
    CAACAGGGAC CATCTGAATA TCGTGACAAA GATTTTCCTA GATGGGACTA TTGTACAACT
    TCAAGTGAAA AGACCCCTCA AACGGTGATG TTCAGCAGGC ATGCTAATAT ACCTGGAAAC
    CACAGAGTTG CAGATGAAGA CAAAAGCAAG TCTTCAGAAG TTCTGGGTGT GAACTTAGAG
    CTGGGAAATG AAGTCTGGGA TGACTTTGAT GATGAGAGCT TAATAGAAGT TATGAGCCTT
    TCAGCTGATG CTGAGAAGAT GGCTGCATCA GGATTTGGAG ACACTCGTGA TTCAAGTCTC
    GGCGGAAGTA AGCTACCCTT CCAAAAGTCA AGCAGCAGAT TCCAAAGAGA CAACTCAAAC
    AGTTTTGCTT CGTCGCCTGG GAAGCCAGAT GCTTATTTGC GAGATTGTTC CAGGTCCAGC
    TTCGGGCTGT CCTCTGTGGC AGAGATCCCT CAGCGGGCTG AGAATGCAAG TCTTGCCAAT
    TTACAAGAGA GGAGACCGCT GACTCTCTCA CCAGTGATTG AGAGGATGTG CTTTGCCCAC
    TCTAAGAAAA CACCACAGTC TCCCAAGTTT AAGGAAGTGG ATATTTTTAT TGGAAACAGT
    GGCTCTAAAA AGGAAATTGA TCTTAGTAAG TATTATCCTG ATGATGCAGC TGAAGAAATG
    AAGGCTCTTC TGGGAATATT TAATGGCATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110454.1
    CDS520..4755
    Translation

    Target ORF information:

    RefSeq Version XM_030254594.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAGAA TGTTAACATC AAACTTGAAG ATCATTAATG AAGACACAAA TTGTATTTCA 
    CCAACACAAA AAATCCATTT TTCCTATAAT GTACATGAAC AGGATTACCT AAATTTAGGA
    GGATCAAATA ACAATGACAT GTCCCATGTA GCTGGGAAGC TGATGTATGG TTCTTCTCAG
    AAATATAAAA ATCACATGGG TGCTAAGAGT CCATCTGCAA GGAGTAGTCC TGGTGATACA
    AAATTACATG ATGTGGCAGA AGACAGACAG GGCACATCAG CATTCAAAAA AAGACTGTCT
    AGAACATGTG ACAGTGAACA TGACTGTGAC TATGCTGATG GAAGTTTAAA CTTGAGTTCT
    CATATCAGCC CAGTGAAACT TACCCAAACG AAAATAAGCA AAGAGAATGC ATGGACGTGT
    AGCAATAGTA AGCAGAAACG TCAGTATTCT ACAAACAAAT TCAAAGCAAA TGATGCTTTT
    TCTGCTTCTG GAATTGGAAA AGACATATTC AAAGCACCAT CATTTCCTGC TGCATCTCAG
    CCTCATGATA TTCAAGGAAT AACCCCAAAT GGCTTAGGTT CTTTGAAAGC TGTCACGGAA
    ATCCCGGCAA AATTTAGAAA TATATTCAAA GAATTCCCTT ACTTCAACTA TATACAATCC
    AAAGCCTTTG ATGATCTTCT TTACACAGAT AGGAATTTTG TGATTTGTGC CCCCACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAGCTAGCG ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGCTAA ACATGAAGAT TGTTTACATG GCACCAATAA AAGCCCTTTG TAGTCAGCGG
    TTTGATGACT GGAAAGAAAA GTTTGGACCA GTAGGCTTGA ATTGTAAAGA ACTTACTGGT
    GATACAGTAA TGGATGACCT GTTTGAGATT CAGCATGCAA ATATAATTAT AACAACTCCA
    GAGAAGTGGG ACAGTGTGAC TAGGAAGTGG AGAGACAACT CTTTTATCCA ACTGGTTCGA
    CTGTTTCTCA TTGATGAGGT GCATGTTATT AAAGATGAAA ATCGTGGTCC AACTCTTGAA
    GTTGTAGTTA GCAGAATGAA AACTGTACAG TCTTTATCTA GGGATTTAGA AAGTGCCAGC
    CCTGTTCCAG TGAGATTTGT AGCTGTGTCT GCAACAATTC CCAATGCTGA GGATATCGCA
    GAATGGCTTT CAGATGGTGA GAGACCTGCT GTATGTCTCA AAATGGATGA GAGCCATAGA
    CCTGTGAAAC TTCAGAAAGT AGTCCTGGGT TTTCCCTGTA GTAGTAGCCA AACTGAATTT
    AAGTTTGATT TGGCCCTCAA CTATAAAGTG TACAGTGTTA TTCGAACATA CTCCGATCAG
    AAGCCCACAC TTGTGTTTTG TTCAACAAGG AAAGGTGTGC AGCAGGCTGC TTCTGTTCTT
    GTGAAGGATG CTAAGTTTAT CATCTCTGTG GAGCAGAAAC TACGGTTGCA GAAGTCTGCA
    TATTCTATAA GGGATTCAAA GCTGAAAGAT ACCTTGGTAT ATGGTGTTGG TTACCATCAT
    GCTGGAATGG AGCTGTCAGA TAGAAAATTG GTTGAAGGAT TGTTTACCTC TGGAGATCTA
    CCAGTTCTTT TCACTACCAG TACTCTAGCT ATGGGCATGA ACATGCCGGC TCACCTGGTT
    GTTATAAAGT CTACCATGCA CTACAGTGGA GGTGTGTTTG AAGAGTACAG CGAGACCGAC
    ATCCTGCAGA TGATTGGGAG AGCTGGCAGA CCCCAGTTTG ACACTACAGC TACTGCAGTC
    ATTATGACTC GACTAAGTAC AAGAGAGAAG TACGTGCAGA TGCTAGCTTG TAACGACACT
    GTAGAGAGCA GTTTGCACAG ACATCTTATT GAACATTTAA ATGCAGAAAT AGTGCTGCAT
    ACCATCACAG ACGTGAACAT TGCTCTGGAC TGGATCCGAT CAACAATGCT GTATATCAGA
    GCTCTGAAAA ACCCATCTCA CTATGGTTTT TCATCTGGAT TGAACAAAGA TGGGATTGAA
    GCAAAATTAC AAGAATTATG TTTGAAGAAT CTGAAAGATT TATCATCACT AGACCTGATA
    AAGATGGATG AAGATGTTAA TTTTAAACCA ACAGAGGCAG GAAGACTAAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT CAAGAAATTT TGTGCAATCA GTGGAAAAGA AACTTTATTG
    GATTTAATTT CAATGATATC GAGCTGCAAT GAGTTTCTAG ATGTGCAGCT GAGGATAAGT
    GAAAAGAGAA TACTGAACAC CTTAAACAAA GACCCAAACC GGATAACTAT CAGATTTCCA
    ATGGCAGAAA GAATTAAAAC AAGAGAAATG AAAGTAAACT GTCTTATTCA GGCTCAGCTA
    GGATGCATTC CTATACAAGA TTTTGCTTTG ACACAAGATA CAGTGAAGAT TTTCAGAAAT
    GGCTCACGAA TTGCAAGATG GTTGTCAGAT TTTGTGGCTG CTCAGGAAAA GAAGTTTGCT
    GTGCTTTTAA ACAGTGTGAT TTTAACTAAA TGTTTTAAGT GTAAACTCTG GGAAAACTCT
    AAACATGTAT CAAAACAGCT GGATAAAATT GGCATATCAT TGTCAAATAC CATGGTAAAT
    GCAGGCTTAA CTTCTTTTAA AAAAATAGAA GAAGCAAATG CAAGAGAACT TGAACTGATT
    TTAAATAGAC ACCCACCATT TGGGACCCAG ATAAAAGAAG CTGTGGCGCA TCTGCCGAAG
    TATGAGCTTG AGGTGGAGCA GATTGCAAGA TACAGTGATA TCAAGGCAGA AATACTGGTG
    ACCATTATAT TAAGAAACTT TGAGCAGCTG CAAACTAAAA GAACAGCACC AGACTTCCAC
    TATGCCACCT TAATCATAGG TGATGCAGAT AACCAAGTCG TTTTTAAGCA CAAAATTATG
    GATTCTGTCT TGTTAAAAAG TGGAAATTGG GTTAAAAAAA TTGATGTGAA GAGAGCTCTT
    ATATCAGAAG ATCTCAGCAT AAATCTAATT AGTTCTGATT ATGTTGGTCT TGATATTCAC
    CAGAAATTTA CAGTCTTTTA TTTCGGACCC AGGAAGTTTG TAAATGAAAC AGCTATGGAA
    AGAAGCTCAG AAACAGATAT TTCTCATTCT GACTACTCAG GTAGAGCCAC TGCAACAGGA
    TCCAGTAAAG GAATGGCTAC CTGCAAAAAA CCTGGGAACA GAGAATGCCA TCATCACTGT
    AAAAATAAAC ACGCATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA AAAGCCGGAA
    GTTAAAGAGT CAGCAATGTC TTCATATCTG TCTGACTTAA AAAGCAGAGA TGCTGTTTCT
    TCTCTTCCTC TAGCCAAGCG ACTCAAGATA CAGATGAACA AATCTCAAAA TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAGGCCTTCC CTTTCCAGTA TATCAAGGTC AGAATATTTA
    AATACCCCTG AATTGTCAAT ATTAGAACAG AGGAATCAGC ATGAAATCTA TGGAAAAGTT
    CAACAGGGAC CATCTGAATA TCGTGACAAA GATTTTCCTA GATGGGACTA TTGTACAACT
    TCAAGTGAAA AGACCCCTCA AACGGTGATG TTCAGCAGGC ATGCTAATAT ACCTGGAAAC
    CACAGAGTTG CAGATGAAGA CAAAAGCAAG TCTTCAGAAG TTCTGGGTGT GAACTTAGAG
    CTGGGAAATG AAGTCTGGGA TGACTTTGAT GATGAGAGCT TAATAGAAGT TATGAGCCTT
    TCAGCTGATG CTGAGAAGAT GGCTGCATCA GGATTTGGAG ACACTCGTGA TTCAAGTCTC
    GGCGGAAGTA AGCTACCCTT CCAAAAGTCA AGCAGCAGAT TCCAAAGAGA CAACTCAAAC
    AGTTTTGCTT CGTCGCCTGG GAAGCCAGAT GCTTATTTGC GAGATTGTTC CAGGTCCAGC
    TTCGGGCTGT CCTCTGTGGC AGAGATCCCT CAGCGGGCTG AGAATGCAAG TCTTGCCAAT
    TTACAAGAGA GGAGACCGCT GACTCTCTCA CCAGTGATTG AGAGGATGTG CTTTGCCCAC
    TCTAAGAAAA CACCACAGTC TCCCAAGTTT AAGGAAGTGG ATATTTTTAT TGGAAACAGT
    GGCTCTAAAA AGGAAATTGA TCTTAGTAAG TATTATCCTG ATGATGCAGC TGAAGAAATG
    AAGGCTCTTC TGGGAATATT TAATGGCATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81398
    Clone ID Related Accession (Same CDS sequence) XM_030254594.1 , XM_017320932.1
    Accession Version XM_017320932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAGAA TGTTAACATC AAACTTGAAG ATCATTAATG AAGACACAAA TTGTATTTCA 
    CCAACACAAA AAATCCATTT TTCCTATAAT GTACATGAAC AGGATTACCT AAATTTAGGA
    GGATCAAATA ACAATGACAT GTCCCATGTA GCTGGGAAGC TGATGTATGG TTCTTCTCAG
    AAATATAAAA ATCACATGGG TGCTAAGAGT CCATCTGCAA GGAGTAGTCC TGGTGATACA
    AAATTACATG ATGTGGCAGA AGACAGACAG GGCACATCAG CATTCAAAAA AAGACTGTCT
    AGAACATGTG ACAGTGAACA TGACTGTGAC TATGCTGATG GAAGTTTAAA CTTGAGTTCT
    CATATCAGCC CAGTGAAACT TACCCAAACG AAAATAAGCA AAGAGAATGC ATGGACGTGT
    AGCAATAGTA AGCAGAAACG TCAGTATTCT ACAAACAAAT TCAAAGCAAA TGATGCTTTT
    TCTGCTTCTG GAATTGGAAA AGACATATTC AAAGCACCAT CATTTCCTGC TGCATCTCAG
    CCTCATGATA TTCAAGGAAT AACCCCAAAT GGCTTAGGTT CTTTGAAAGC TGTCACGGAA
    ATCCCGGCAA AATTTAGAAA TATATTCAAA GAATTCCCTT ACTTCAACTA TATACAATCC
    AAAGCCTTTG ATGATCTTCT TTACACAGAT AGGAATTTTG TGATTTGTGC CCCCACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAGCTAGCG ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGCTAA ACATGAAGAT TGTTTACATG GCACCAATAA AAGCCCTTTG TAGTCAGCGG
    TTTGATGACT GGAAAGAAAA GTTTGGACCA GTAGGCTTGA ATTGTAAAGA ACTTACTGGT
    GATACAGTAA TGGATGACCT GTTTGAGATT CAGCATGCAA ATATAATTAT AACAACTCCA
    GAGAAGTGGG ACAGTGTGAC TAGGAAGTGG AGAGACAACT CTTTTATCCA ACTGGTTCGA
    CTGTTTCTCA TTGATGAGGT GCATGTTATT AAAGATGAAA ATCGTGGTCC AACTCTTGAA
    GTTGTAGTTA GCAGAATGAA AACTGTACAG TCTTTATCTA GGGATTTAGA AAGTGCCAGC
    CCTGTTCCAG TGAGATTTGT AGCTGTGTCT GCAACAATTC CCAATGCTGA GGATATCGCA
    GAATGGCTTT CAGATGGTGA GAGACCTGCT GTATGTCTCA AAATGGATGA GAGCCATAGA
    CCTGTGAAAC TTCAGAAAGT AGTCCTGGGT TTTCCCTGTA GTAGTAGCCA AACTGAATTT
    AAGTTTGATT TGGCCCTCAA CTATAAAGTG TACAGTGTTA TTCGAACATA CTCCGATCAG
    AAGCCCACAC TTGTGTTTTG TTCAACAAGG AAAGGTGTGC AGCAGGCTGC TTCTGTTCTT
    GTGAAGGATG CTAAGTTTAT CATCTCTGTG GAGCAGAAAC TACGGTTGCA GAAGTCTGCA
    TATTCTATAA GGGATTCAAA GCTGAAAGAT ACCTTGGTAT ATGGTGTTGG TTACCATCAT
    GCTGGAATGG AGCTGTCAGA TAGAAAATTG GTTGAAGGAT TGTTTACCTC TGGAGATCTA
    CCAGTTCTTT TCACTACCAG TACTCTAGCT ATGGGCATGA ACATGCCGGC TCACCTGGTT
    GTTATAAAGT CTACCATGCA CTACAGTGGA GGTGTGTTTG AAGAGTACAG CGAGACCGAC
    ATCCTGCAGA TGATTGGGAG AGCTGGCAGA CCCCAGTTTG ACACTACAGC TACTGCAGTC
    ATTATGACTC GACTAAGTAC AAGAGAGAAG TACGTGCAGA TGCTAGCTTG TAACGACACT
    GTAGAGAGCA GTTTGCACAG ACATCTTATT GAACATTTAA ATGCAGAAAT AGTGCTGCAT
    ACCATCACAG ACGTGAACAT TGCTCTGGAC TGGATCCGAT CAACAATGCT GTATATCAGA
    GCTCTGAAAA ACCCATCTCA CTATGGTTTT TCATCTGGAT TGAACAAAGA TGGGATTGAA
    GCAAAATTAC AAGAATTATG TTTGAAGAAT CTGAAAGATT TATCATCACT AGACCTGATA
    AAGATGGATG AAGATGTTAA TTTTAAACCA ACAGAGGCAG GAAGACTAAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT CAAGAAATTT TGTGCAATCA GTGGAAAAGA AACTTTATTG
    GATTTAATTT CAATGATATC GAGCTGCAAT GAGTTTCTAG ATGTGCAGCT GAGGATAAGT
    GAAAAGAGAA TACTGAACAC CTTAAACAAA GACCCAAACC GGATAACTAT CAGATTTCCA
    ATGGCAGAAA GAATTAAAAC AAGAGAAATG AAAGTAAACT GTCTTATTCA GGCTCAGCTA
    GGATGCATTC CTATACAAGA TTTTGCTTTG ACACAAGATA CAGTGAAGAT TTTCAGAAAT
    GGCTCACGAA TTGCAAGATG GTTGTCAGAT TTTGTGGCTG CTCAGGAAAA GAAGTTTGCT
    GTGCTTTTAA ACAGTGTGAT TTTAACTAAA TGTTTTAAGT GTAAACTCTG GGAAAACTCT
    AAACATGTAT CAAAACAGCT GGATAAAATT GGCATATCAT TGTCAAATAC CATGGTAAAT
    GCAGGCTTAA CTTCTTTTAA AAAAATAGAA GAAGCAAATG CAAGAGAACT TGAACTGATT
    TTAAATAGAC ACCCACCATT TGGGACCCAG ATAAAAGAAG CTGTGGCGCA TCTGCCGAAG
    TATGAGCTTG AGGTGGAGCA GATTGCAAGA TACAGTGATA TCAAGGCAGA AATACTGGTG
    ACCATTATAT TAAGAAACTT TGAGCAGCTG CAAACTAAAA GAACAGCACC AGACTTCCAC
    TATGCCACCT TAATCATAGG TGATGCAGAT AACCAAGTCG TTTTTAAGCA CAAAATTATG
    GATTCTGTCT TGTTAAAAAG TGGAAATTGG GTTAAAAAAA TTGATGTGAA GAGAGCTCTT
    ATATCAGAAG ATCTCAGCAT AAATCTAATT AGTTCTGATT ATGTTGGTCT TGATATTCAC
    CAGAAATTTA CAGTCTTTTA TTTCGGACCC AGGAAGTTTG TAAATGAAAC AGCTATGGAA
    AGAAGCTCAG AAACAGATAT TTCTCATTCT GACTACTCAG GTAGAGCCAC TGCAACAGGA
    TCCAGTAAAG GAATGGCTAC CTGCAAAAAA CCTGGGAACA GAGAATGCCA TCATCACTGT
    AAAAATAAAC ACGCATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA AAAGCCGGAA
    GTTAAAGAGT CAGCAATGTC TTCATATCTG TCTGACTTAA AAAGCAGAGA TGCTGTTTCT
    TCTCTTCCTC TAGCCAAGCG ACTCAAGATA CAGATGAACA AATCTCAAAA TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAGGCCTTCC CTTTCCAGTA TATCAAGGTC AGAATATTTA
    AATACCCCTG AATTGTCAAT ATTAGAACAG AGGAATCAGC ATGAAATCTA TGGAAAAGTT
    CAACAGGGAC CATCTGAATA TCGTGACAAA GATTTTCCTA GATGGGACTA TTGTACAACT
    TCAAGTGAAA AGACCCCTCA AACGGTGATG TTCAGCAGGC ATGCTAATAT ACCTGGAAAC
    CACAGAGTTG CAGATGAAGA CAAAAGCAAG TCTTCAGAAG TTCTGGGTGT GAACTTAGAG
    CTGGGAAATG AAGTCTGGGA TGACTTTGAT GATGAGAGCT TAATAGAAGT TATGAGCCTT
    TCAGCTGATG CTGAGAAGAT GGCTGCATCA GGATTTGGAG ACACTCGTGA TTCAAGTCTC
    GGCGGAAGTA AGCTACCCTT CCAAAAGTCA AGCAGCAGAT TCCAAAGAGA CAACTCAAAC
    AGTTTTGCTT CGTCGCCTGG GAAGCCAGAT GCTTATTTGC GAGATTGTTC CAGGTCCAGC
    TTCGGGCTGT CCTCTGTGGC AGAGATCCCT CAGCGGGCTG AGAATGCAAG TCTTGCCAAT
    TTACAAGAGA GGAGACCGCT GACTCTCTCA CCAGTGATTG AGAGGATGTG CTTTGCCCAC
    TCTAAGAAAA CACCACAGTC TCCCAAGTTT AAGGAAGTGG ATATTTTTAT TGGAAACAGT
    GGCTCTAAAA AGGAAATTGA TCTTAGTAAG TATTATCCTG ATGATGCAGC TGAAGAAATG
    AAGGCTCTTC TGGGAATATT TAATGGCATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176421.1
    CDS167..4402
    Translation

    Target ORF information:

    RefSeq Version XM_017320932.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAGAA TGTTAACATC AAACTTGAAG ATCATTAATG AAGACACAAA TTGTATTTCA 
    CCAACACAAA AAATCCATTT TTCCTATAAT GTACATGAAC AGGATTACCT AAATTTAGGA
    GGATCAAATA ACAATGACAT GTCCCATGTA GCTGGGAAGC TGATGTATGG TTCTTCTCAG
    AAATATAAAA ATCACATGGG TGCTAAGAGT CCATCTGCAA GGAGTAGTCC TGGTGATACA
    AAATTACATG ATGTGGCAGA AGACAGACAG GGCACATCAG CATTCAAAAA AAGACTGTCT
    AGAACATGTG ACAGTGAACA TGACTGTGAC TATGCTGATG GAAGTTTAAA CTTGAGTTCT
    CATATCAGCC CAGTGAAACT TACCCAAACG AAAATAAGCA AAGAGAATGC ATGGACGTGT
    AGCAATAGTA AGCAGAAACG TCAGTATTCT ACAAACAAAT TCAAAGCAAA TGATGCTTTT
    TCTGCTTCTG GAATTGGAAA AGACATATTC AAAGCACCAT CATTTCCTGC TGCATCTCAG
    CCTCATGATA TTCAAGGAAT AACCCCAAAT GGCTTAGGTT CTTTGAAAGC TGTCACGGAA
    ATCCCGGCAA AATTTAGAAA TATATTCAAA GAATTCCCTT ACTTCAACTA TATACAATCC
    AAAGCCTTTG ATGATCTTCT TTACACAGAT AGGAATTTTG TGATTTGTGC CCCCACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAGCTAGCG ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGCTAA ACATGAAGAT TGTTTACATG GCACCAATAA AAGCCCTTTG TAGTCAGCGG
    TTTGATGACT GGAAAGAAAA GTTTGGACCA GTAGGCTTGA ATTGTAAAGA ACTTACTGGT
    GATACAGTAA TGGATGACCT GTTTGAGATT CAGCATGCAA ATATAATTAT AACAACTCCA
    GAGAAGTGGG ACAGTGTGAC TAGGAAGTGG AGAGACAACT CTTTTATCCA ACTGGTTCGA
    CTGTTTCTCA TTGATGAGGT GCATGTTATT AAAGATGAAA ATCGTGGTCC AACTCTTGAA
    GTTGTAGTTA GCAGAATGAA AACTGTACAG TCTTTATCTA GGGATTTAGA AAGTGCCAGC
    CCTGTTCCAG TGAGATTTGT AGCTGTGTCT GCAACAATTC CCAATGCTGA GGATATCGCA
    GAATGGCTTT CAGATGGTGA GAGACCTGCT GTATGTCTCA AAATGGATGA GAGCCATAGA
    CCTGTGAAAC TTCAGAAAGT AGTCCTGGGT TTTCCCTGTA GTAGTAGCCA AACTGAATTT
    AAGTTTGATT TGGCCCTCAA CTATAAAGTG TACAGTGTTA TTCGAACATA CTCCGATCAG
    AAGCCCACAC TTGTGTTTTG TTCAACAAGG AAAGGTGTGC AGCAGGCTGC TTCTGTTCTT
    GTGAAGGATG CTAAGTTTAT CATCTCTGTG GAGCAGAAAC TACGGTTGCA GAAGTCTGCA
    TATTCTATAA GGGATTCAAA GCTGAAAGAT ACCTTGGTAT ATGGTGTTGG TTACCATCAT
    GCTGGAATGG AGCTGTCAGA TAGAAAATTG GTTGAAGGAT TGTTTACCTC TGGAGATCTA
    CCAGTTCTTT TCACTACCAG TACTCTAGCT ATGGGCATGA ACATGCCGGC TCACCTGGTT
    GTTATAAAGT CTACCATGCA CTACAGTGGA GGTGTGTTTG AAGAGTACAG CGAGACCGAC
    ATCCTGCAGA TGATTGGGAG AGCTGGCAGA CCCCAGTTTG ACACTACAGC TACTGCAGTC
    ATTATGACTC GACTAAGTAC AAGAGAGAAG TACGTGCAGA TGCTAGCTTG TAACGACACT
    GTAGAGAGCA GTTTGCACAG ACATCTTATT GAACATTTAA ATGCAGAAAT AGTGCTGCAT
    ACCATCACAG ACGTGAACAT TGCTCTGGAC TGGATCCGAT CAACAATGCT GTATATCAGA
    GCTCTGAAAA ACCCATCTCA CTATGGTTTT TCATCTGGAT TGAACAAAGA TGGGATTGAA
    GCAAAATTAC AAGAATTATG TTTGAAGAAT CTGAAAGATT TATCATCACT AGACCTGATA
    AAGATGGATG AAGATGTTAA TTTTAAACCA ACAGAGGCAG GAAGACTAAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT CAAGAAATTT TGTGCAATCA GTGGAAAAGA AACTTTATTG
    GATTTAATTT CAATGATATC GAGCTGCAAT GAGTTTCTAG ATGTGCAGCT GAGGATAAGT
    GAAAAGAGAA TACTGAACAC CTTAAACAAA GACCCAAACC GGATAACTAT CAGATTTCCA
    ATGGCAGAAA GAATTAAAAC AAGAGAAATG AAAGTAAACT GTCTTATTCA GGCTCAGCTA
    GGATGCATTC CTATACAAGA TTTTGCTTTG ACACAAGATA CAGTGAAGAT TTTCAGAAAT
    GGCTCACGAA TTGCAAGATG GTTGTCAGAT TTTGTGGCTG CTCAGGAAAA GAAGTTTGCT
    GTGCTTTTAA ACAGTGTGAT TTTAACTAAA TGTTTTAAGT GTAAACTCTG GGAAAACTCT
    AAACATGTAT CAAAACAGCT GGATAAAATT GGCATATCAT TGTCAAATAC CATGGTAAAT
    GCAGGCTTAA CTTCTTTTAA AAAAATAGAA GAAGCAAATG CAAGAGAACT TGAACTGATT
    TTAAATAGAC ACCCACCATT TGGGACCCAG ATAAAAGAAG CTGTGGCGCA TCTGCCGAAG
    TATGAGCTTG AGGTGGAGCA GATTGCAAGA TACAGTGATA TCAAGGCAGA AATACTGGTG
    ACCATTATAT TAAGAAACTT TGAGCAGCTG CAAACTAAAA GAACAGCACC AGACTTCCAC
    TATGCCACCT TAATCATAGG TGATGCAGAT AACCAAGTCG TTTTTAAGCA CAAAATTATG
    GATTCTGTCT TGTTAAAAAG TGGAAATTGG GTTAAAAAAA TTGATGTGAA GAGAGCTCTT
    ATATCAGAAG ATCTCAGCAT AAATCTAATT AGTTCTGATT ATGTTGGTCT TGATATTCAC
    CAGAAATTTA CAGTCTTTTA TTTCGGACCC AGGAAGTTTG TAAATGAAAC AGCTATGGAA
    AGAAGCTCAG AAACAGATAT TTCTCATTCT GACTACTCAG GTAGAGCCAC TGCAACAGGA
    TCCAGTAAAG GAATGGCTAC CTGCAAAAAA CCTGGGAACA GAGAATGCCA TCATCACTGT
    AAAAATAAAC ACGCATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA AAAGCCGGAA
    GTTAAAGAGT CAGCAATGTC TTCATATCTG TCTGACTTAA AAAGCAGAGA TGCTGTTTCT
    TCTCTTCCTC TAGCCAAGCG ACTCAAGATA CAGATGAACA AATCTCAAAA TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAGGCCTTCC CTTTCCAGTA TATCAAGGTC AGAATATTTA
    AATACCCCTG AATTGTCAAT ATTAGAACAG AGGAATCAGC ATGAAATCTA TGGAAAAGTT
    CAACAGGGAC CATCTGAATA TCGTGACAAA GATTTTCCTA GATGGGACTA TTGTACAACT
    TCAAGTGAAA AGACCCCTCA AACGGTGATG TTCAGCAGGC ATGCTAATAT ACCTGGAAAC
    CACAGAGTTG CAGATGAAGA CAAAAGCAAG TCTTCAGAAG TTCTGGGTGT GAACTTAGAG
    CTGGGAAATG AAGTCTGGGA TGACTTTGAT GATGAGAGCT TAATAGAAGT TATGAGCCTT
    TCAGCTGATG CTGAGAAGAT GGCTGCATCA GGATTTGGAG ACACTCGTGA TTCAAGTCTC
    GGCGGAAGTA AGCTACCCTT CCAAAAGTCA AGCAGCAGAT TCCAAAGAGA CAACTCAAAC
    AGTTTTGCTT CGTCGCCTGG GAAGCCAGAT GCTTATTTGC GAGATTGTTC CAGGTCCAGC
    TTCGGGCTGT CCTCTGTGGC AGAGATCCCT CAGCGGGCTG AGAATGCAAG TCTTGCCAAT
    TTACAAGAGA GGAGACCGCT GACTCTCTCA CCAGTGATTG AGAGGATGTG CTTTGCCCAC
    TCTAAGAAAA CACCACAGTC TCCCAAGTTT AAGGAAGTGG ATATTTTTAT TGGAAACAGT
    GGCTCTAAAA AGGAAATTGA TCTTAGTAAG TATTATCCTG ATGATGCAGC TGAAGAAATG
    AAGGCTCTTC TGGGAATATT TAATGGCATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88942
    Clone ID Related Accession (Same CDS sequence) XM_030254590.1
    Accession Version XM_030254590.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GACTGTCTAG AACATGTGAC AGTGAACATG ACTGTGACTA TGCTGATGGA
    AGTTTAAACT TGAGTTCTCA TATCAGCCCA GTGAAACTTA CCCAAACGAA AATAAGCAAA
    GAGAATGCAT GGACGTGTAG CAATAGTAAG CAGAAACGTC AGTATTCTAC AAACAAATTC
    AAAGCAAATG ATGCTTTTTC TGCTTCTGGA ATTGGAAAAG ACATATTCAA AGCACCATCA
    TTTCCTGCTG CATCTCAGCC TCATGATATT CAAGGAATAA CCCCAAATGG CTTAGGTTCT
    TTGAAAGCTG TCACGGAAAT CCCGGCAAAA TTTAGAAATA TATTCAAAGA ATTCCCTTAC
    TTCAACTATA TACAATCCAA AGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCCC CCACTGGTTC TGGGAAAACT GTAGTGTTTG AGCTAGCGAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGCTAAAC ATGAAGATTG TTTACATGGC ACCAATAAAA
    GCCCTTTGTA GTCAGCGGTT TGATGACTGG AAAGAAAAGT TTGGACCAGT AGGCTTGAAT
    TGTAAAGAAC TTACTGGTGA TACAGTAATG GATGACCTGT TTGAGATTCA GCATGCAAAT
    ATAATTATAA CAACTCCAGA GAAGTGGGAC AGTGTGACTA GGAAGTGGAG AGACAACTCT
    TTTATCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTGC ATGTTATTAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TTTATCTAGG
    GATTTAGAAA GTGCCAGCCC TGTTCCAGTG AGATTTGTAG CTGTGTCTGC AACAATTCCC
    AATGCTGAGG ATATCGCAGA ATGGCTTTCA GATGGTGAGA GACCTGCTGT ATGTCTCAAA
    ATGGATGAGA GCCATAGACC TGTGAAACTT CAGAAAGTAG TCCTGGGTTT TCCCTGTAGT
    AGTAGCCAAA CTGAATTTAA GTTTGATTTG GCCCTCAACT ATAAAGTGTA CAGTGTTATT
    CGAACATACT CCGATCAGAA GCCCACACTT GTGTTTTGTT CAACAAGGAA AGGTGTGCAG
    CAGGCTGCTT CTGTTCTTGT GAAGGATGCT AAGTTTATCA TCTCTGTGGA GCAGAAACTA
    CGGTTGCAGA AGTCTGCATA TTCTATAAGG GATTCAAAGC TGAAAGATAC CTTGGTATAT
    GGTGTTGGTT ACCATCATGC TGGAATGGAG CTGTCAGATA GAAAATTGGT TGAAGGATTG
    TTTACCTCTG GAGATCTACC AGTTCTTTTC ACTACCAGTA CTCTAGCTAT GGGCATGAAC
    ATGCCGGCTC ACCTGGTTGT TATAAAGTCT ACCATGCACT ACAGTGGAGG TGTGTTTGAA
    GAGTACAGCG AGACCGACAT CCTGCAGATG ATTGGGAGAG CTGGCAGACC CCAGTTTGAC
    ACTACAGCTA CTGCAGTCAT TATGACTCGA CTAAGTACAA GAGAGAAGTA CGTGCAGATG
    CTAGCTTGTA ACGACACTGT AGAGAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAAATAG TGCTGCATAC CATCACAGAC GTGAACATTG CTCTGGACTG GATCCGATCA
    ACAATGCTGT ATATCAGAGC TCTGAAAAAC CCATCTCACT ATGGTTTTTC ATCTGGATTG
    AACAAAGATG GGATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAAAGATTTA
    TCATCACTAG ACCTGATAAA GATGGATGAA GATGTTAATT TTAAACCAAC AGAGGCAGGA
    AGACTAATGG CTTGGTATTA TATTACATTT GAGACAGTCA AGAAATTTTG TGCAATCAGT
    GGAAAAGAAA CTTTATTGGA TTTAATTTCA ATGATATCGA GCTGCAATGA GTTTCTAGAT
    GTGCAGCTGA GGATAAGTGA AAAGAGAATA CTGAACACCT TAAACAAAGA CCCAAACCGG
    ATAACTATCA GATTTCCAAT GGCAGAAAGA ATTAAAACAA GAGAAATGAA AGTAAACTGT
    CTTATTCAGG CTCAGCTAGG ATGCATTCCT ATACAAGATT TTGCTTTGAC ACAAGATACA
    GTGAAGATTT TCAGAAATGG CTCACGAATT GCAAGATGGT TGTCAGATTT TGTGGCTGCT
    CAGGAAAAGA AGTTTGCTGT GCTTTTAAAC AGTGTGATTT TAACTAAATG TTTTAAGTGT
    AAACTCTGGG AAAACTCTAA ACATGTATCA AAACAGCTGG ATAAAATTGG CATATCATTG
    TCAAATACCA TGGTAAATGC AGGCTTAACT TCTTTTAAAA AAATAGAAGA AGCAAATGCA
    AGAGAACTTG AACTGATTTT AAATAGACAC CCACCATTTG GGACCCAGAT AAAAGAAGCT
    GTGGCGCATC TGCCGAAGTA TGAGCTTGAG GTGGAGCAGA TTGCAAGATA CAGTGATATC
    AAGGCAGAAA TACTGGTGAC CATTATATTA AGAAACTTTG AGCAGCTGCA AACTAAAAGA
    ACAGCACCAG ACTTCCACTA TGCCACCTTA ATCATAGGTG ATGCAGATAA CCAAGTCGTT
    TTTAAGCACA AAATTATGGA TTCTGTCTTG TTAAAAAGTG GAAATTGGGT TAAAAAAATT
    GATGTGAAGA GAGCTCTTAT ATCAGAAGAT CTCAGCATAA ATCTAATTAG TTCTGATTAT
    GTTGGTCTTG ATATTCACCA GAAATTTACA GTCTTTTATT TCGGACCCAG GAAGTTTGTA
    AATGAAACAG CTATGGAAAG AAGCTCAGAA ACAGATATTT CTCATTCTGA CTACTCAGGT
    AGAGCCACTG CAACAGGATC CAGTAAAGGA ATGGCTACCT GCAAAAAACC TGGGAACAGA
    GAATGCCATC ATCACTGTAA AAATAAACAC GCATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAAA AGCCGGAAGT TAAAGAGTCA GCAATGTCTT CATATCTGTC TGACTTAAAA
    AGCAGAGATG CTGTTTCTTC TCTTCCTCTA GCCAAGCGAC TCAAGATACA GATGAACAAA
    TCTCAAAATG TGGACCTTAA AGAGTTTGGT TTTACTCCAA GGCCTTCCCT TTCCAGTATA
    TCAAGGTCAG AATATTTAAA TACCCCTGAA TTGTCAATAT TAGAACAGAG GAATCAGCAT
    GAAATCTATG GAAAAGTTCA ACAGGGACCA TCTGAATATC GTGACAAAGA TTTTCCTAGA
    TGGGACTATT GTACAACTTC AAGTGAAAAG ACCCCTCAAA CGGTGATGTT CAGCAGGCAT
    GCTAATATAC CTGGAAACCA CAGAGTTGCA GATGAAGACA AAAGCAAGTC TTCAGAAGTT
    CTGGGTGTGA ACTTAGAGCT GGGAAATGAA GTCTGGGATG ACTTTGATGA TGAGAGCTTA
    ATAGAAGTTA TGAGCCTTTC AGCTGATGCT GAGAAGATGG CTGCATCAGG ATTTGGAGAC
    ACTCGTGATT CAAGTCTCGG CGGAAGTAAG CTACCCTTCC AAAAGTCAAG CAGCAGATTC
    CAAAGAGACA ACTCAAACAG TTTTGCTTCG TCGCCTGGGA AGCCAGATGC TTATTTGCGA
    GATTGTTCCA GGTCCAGCTT CGGGCTGTCC TCTGTGGCAG AGATCCCTCA GCGGGCTGAG
    AATGCAAGTC TTGCCAATTT ACAAGAGAGG AGACCGCTGA CTCTCTCACC AGTGATTGAG
    AGGATGTGCT TTGCCCACTC TAAGAAAACA CCACAGTCTC CCAAGTTTAA GGAAGTGGAT
    ATTTTTATTG GAAACAGTGG CTCTAAAAAG GAAATTGATC TTAGTAAGTA TTATCCTGAT
    GATGCAGCTG AAGAAATGAA GGCTCTTCTG GGAATATTTA ATGGCATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110450.1
    CDS288..4661
    Translation

    Target ORF information:

    RefSeq Version XM_030254590.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254590.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GACTGTCTAG AACATGTGAC AGTGAACATG ACTGTGACTA TGCTGATGGA
    AGTTTAAACT TGAGTTCTCA TATCAGCCCA GTGAAACTTA CCCAAACGAA AATAAGCAAA
    GAGAATGCAT GGACGTGTAG CAATAGTAAG CAGAAACGTC AGTATTCTAC AAACAAATTC
    AAAGCAAATG ATGCTTTTTC TGCTTCTGGA ATTGGAAAAG ACATATTCAA AGCACCATCA
    TTTCCTGCTG CATCTCAGCC TCATGATATT CAAGGAATAA CCCCAAATGG CTTAGGTTCT
    TTGAAAGCTG TCACGGAAAT CCCGGCAAAA TTTAGAAATA TATTCAAAGA ATTCCCTTAC
    TTCAACTATA TACAATCCAA AGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCCC CCACTGGTTC TGGGAAAACT GTAGTGTTTG AGCTAGCGAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGCTAAAC ATGAAGATTG TTTACATGGC ACCAATAAAA
    GCCCTTTGTA GTCAGCGGTT TGATGACTGG AAAGAAAAGT TTGGACCAGT AGGCTTGAAT
    TGTAAAGAAC TTACTGGTGA TACAGTAATG GATGACCTGT TTGAGATTCA GCATGCAAAT
    ATAATTATAA CAACTCCAGA GAAGTGGGAC AGTGTGACTA GGAAGTGGAG AGACAACTCT
    TTTATCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTGC ATGTTATTAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TTTATCTAGG
    GATTTAGAAA GTGCCAGCCC TGTTCCAGTG AGATTTGTAG CTGTGTCTGC AACAATTCCC
    AATGCTGAGG ATATCGCAGA ATGGCTTTCA GATGGTGAGA GACCTGCTGT ATGTCTCAAA
    ATGGATGAGA GCCATAGACC TGTGAAACTT CAGAAAGTAG TCCTGGGTTT TCCCTGTAGT
    AGTAGCCAAA CTGAATTTAA GTTTGATTTG GCCCTCAACT ATAAAGTGTA CAGTGTTATT
    CGAACATACT CCGATCAGAA GCCCACACTT GTGTTTTGTT CAACAAGGAA AGGTGTGCAG
    CAGGCTGCTT CTGTTCTTGT GAAGGATGCT AAGTTTATCA TCTCTGTGGA GCAGAAACTA
    CGGTTGCAGA AGTCTGCATA TTCTATAAGG GATTCAAAGC TGAAAGATAC CTTGGTATAT
    GGTGTTGGTT ACCATCATGC TGGAATGGAG CTGTCAGATA GAAAATTGGT TGAAGGATTG
    TTTACCTCTG GAGATCTACC AGTTCTTTTC ACTACCAGTA CTCTAGCTAT GGGCATGAAC
    ATGCCGGCTC ACCTGGTTGT TATAAAGTCT ACCATGCACT ACAGTGGAGG TGTGTTTGAA
    GAGTACAGCG AGACCGACAT CCTGCAGATG ATTGGGAGAG CTGGCAGACC CCAGTTTGAC
    ACTACAGCTA CTGCAGTCAT TATGACTCGA CTAAGTACAA GAGAGAAGTA CGTGCAGATG
    CTAGCTTGTA ACGACACTGT AGAGAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAAATAG TGCTGCATAC CATCACAGAC GTGAACATTG CTCTGGACTG GATCCGATCA
    ACAATGCTGT ATATCAGAGC TCTGAAAAAC CCATCTCACT ATGGTTTTTC ATCTGGATTG
    AACAAAGATG GGATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAAAGATTTA
    TCATCACTAG ACCTGATAAA GATGGATGAA GATGTTAATT TTAAACCAAC AGAGGCAGGA
    AGACTAATGG CTTGGTATTA TATTACATTT GAGACAGTCA AGAAATTTTG TGCAATCAGT
    GGAAAAGAAA CTTTATTGGA TTTAATTTCA ATGATATCGA GCTGCAATGA GTTTCTAGAT
    GTGCAGCTGA GGATAAGTGA AAAGAGAATA CTGAACACCT TAAACAAAGA CCCAAACCGG
    ATAACTATCA GATTTCCAAT GGCAGAAAGA ATTAAAACAA GAGAAATGAA AGTAAACTGT
    CTTATTCAGG CTCAGCTAGG ATGCATTCCT ATACAAGATT TTGCTTTGAC ACAAGATACA
    GTGAAGATTT TCAGAAATGG CTCACGAATT GCAAGATGGT TGTCAGATTT TGTGGCTGCT
    CAGGAAAAGA AGTTTGCTGT GCTTTTAAAC AGTGTGATTT TAACTAAATG TTTTAAGTGT
    AAACTCTGGG AAAACTCTAA ACATGTATCA AAACAGCTGG ATAAAATTGG CATATCATTG
    TCAAATACCA TGGTAAATGC AGGCTTAACT TCTTTTAAAA AAATAGAAGA AGCAAATGCA
    AGAGAACTTG AACTGATTTT AAATAGACAC CCACCATTTG GGACCCAGAT AAAAGAAGCT
    GTGGCGCATC TGCCGAAGTA TGAGCTTGAG GTGGAGCAGA TTGCAAGATA CAGTGATATC
    AAGGCAGAAA TACTGGTGAC CATTATATTA AGAAACTTTG AGCAGCTGCA AACTAAAAGA
    ACAGCACCAG ACTTCCACTA TGCCACCTTA ATCATAGGTG ATGCAGATAA CCAAGTCGTT
    TTTAAGCACA AAATTATGGA TTCTGTCTTG TTAAAAAGTG GAAATTGGGT TAAAAAAATT
    GATGTGAAGA GAGCTCTTAT ATCAGAAGAT CTCAGCATAA ATCTAATTAG TTCTGATTAT
    GTTGGTCTTG ATATTCACCA GAAATTTACA GTCTTTTATT TCGGACCCAG GAAGTTTGTA
    AATGAAACAG CTATGGAAAG AAGCTCAGAA ACAGATATTT CTCATTCTGA CTACTCAGGT
    AGAGCCACTG CAACAGGATC CAGTAAAGGA ATGGCTACCT GCAAAAAACC TGGGAACAGA
    GAATGCCATC ATCACTGTAA AAATAAACAC GCATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAAA AGCCGGAAGT TAAAGAGTCA GCAATGTCTT CATATCTGTC TGACTTAAAA
    AGCAGAGATG CTGTTTCTTC TCTTCCTCTA GCCAAGCGAC TCAAGATACA GATGAACAAA
    TCTCAAAATG TGGACCTTAA AGAGTTTGGT TTTACTCCAA GGCCTTCCCT TTCCAGTATA
    TCAAGGTCAG AATATTTAAA TACCCCTGAA TTGTCAATAT TAGAACAGAG GAATCAGCAT
    GAAATCTATG GAAAAGTTCA ACAGGGACCA TCTGAATATC GTGACAAAGA TTTTCCTAGA
    TGGGACTATT GTACAACTTC AAGTGAAAAG ACCCCTCAAA CGGTGATGTT CAGCAGGCAT
    GCTAATATAC CTGGAAACCA CAGAGTTGCA GATGAAGACA AAAGCAAGTC TTCAGAAGTT
    CTGGGTGTGA ACTTAGAGCT GGGAAATGAA GTCTGGGATG ACTTTGATGA TGAGAGCTTA
    ATAGAAGTTA TGAGCCTTTC AGCTGATGCT GAGAAGATGG CTGCATCAGG ATTTGGAGAC
    ACTCGTGATT CAAGTCTCGG CGGAAGTAAG CTACCCTTCC AAAAGTCAAG CAGCAGATTC
    CAAAGAGACA ACTCAAACAG TTTTGCTTCG TCGCCTGGGA AGCCAGATGC TTATTTGCGA
    GATTGTTCCA GGTCCAGCTT CGGGCTGTCC TCTGTGGCAG AGATCCCTCA GCGGGCTGAG
    AATGCAAGTC TTGCCAATTT ACAAGAGAGG AGACCGCTGA CTCTCTCACC AGTGATTGAG
    AGGATGTGCT TTGCCCACTC TAAGAAAACA CCACAGTCTC CCAAGTTTAA GGAAGTGGAT
    ATTTTTATTG GAAACAGTGG CTCTAAAAAG GAAATTGATC TTAGTAAGTA TTATCCTGAT
    GATGCAGCTG AAGAAATGAA GGCTCTTCTG GGAATATTTA ATGGCATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88943
    Clone ID Related Accession (Same CDS sequence) XM_030254591.1 , XM_030254592.1
    Accession Version XM_030254591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GCCCAGTGAA ACTTACCCAA ACGAAAATAA GCAAAGAGAA TGCATGGACG
    TGTAGCAATA GTAAGCAGAA ACGTCAGTAT TCTACAAACA AATTCAAAGC AAATGATGCT
    TTTTCTGCTT CTGGAATTGG AAAAGACATA TTCAAAGCAC CATCATTTCC TGCTGCATCT
    CAGCCTCATG ATATTCAAGG AATAACCCCA AATGGCTTAG GTTCTTTGAA AGCTGTCACG
    GAAATCCCGG CAAAATTTAG AAATATATTC AAAGAATTCC CTTACTTCAA CTATATACAA
    TCCAAAGCCT TTGATGATCT TCTTTACACA GATAGGAATT TTGTGATTTG TGCCCCCACT
    GGTTCTGGGA AAACTGTAGT GTTTGAGCTA GCGATAACAA GATTGTTAAT GGAAGTACCA
    TTGCCATGGC TAAACATGAA GATTGTTTAC ATGGCACCAA TAAAAGCCCT TTGTAGTCAG
    CGGTTTGATG ACTGGAAAGA AAAGTTTGGA CCAGTAGGCT TGAATTGTAA AGAACTTACT
    GGTGATACAG TAATGGATGA CCTGTTTGAG ATTCAGCATG CAAATATAAT TATAACAACT
    CCAGAGAAGT GGGACAGTGT GACTAGGAAG TGGAGAGACA ACTCTTTTAT CCAACTGGTT
    CGACTGTTTC TCATTGATGA GGTGCATGTT ATTAAAGATG AAAATCGTGG TCCAACTCTT
    GAAGTTGTAG TTAGCAGAAT GAAAACTGTA CAGTCTTTAT CTAGGGATTT AGAAAGTGCC
    AGCCCTGTTC CAGTGAGATT TGTAGCTGTG TCTGCAACAA TTCCCAATGC TGAGGATATC
    GCAGAATGGC TTTCAGATGG TGAGAGACCT GCTGTATGTC TCAAAATGGA TGAGAGCCAT
    AGACCTGTGA AACTTCAGAA AGTAGTCCTG GGTTTTCCCT GTAGTAGTAG CCAAACTGAA
    TTTAAGTTTG ATTTGGCCCT CAACTATAAA GTGTACAGTG TTATTCGAAC ATACTCCGAT
    CAGAAGCCCA CACTTGTGTT TTGTTCAACA AGGAAAGGTG TGCAGCAGGC TGCTTCTGTT
    CTTGTGAAGG ATGCTAAGTT TATCATCTCT GTGGAGCAGA AACTACGGTT GCAGAAGTCT
    GCATATTCTA TAAGGGATTC AAAGCTGAAA GATACCTTGG TATATGGTGT TGGTTACCAT
    CATGCTGGAA TGGAGCTGTC AGATAGAAAA TTGGTTGAAG GATTGTTTAC CTCTGGAGAT
    CTACCAGTTC TTTTCACTAC CAGTACTCTA GCTATGGGCA TGAACATGCC GGCTCACCTG
    GTTGTTATAA AGTCTACCAT GCACTACAGT GGAGGTGTGT TTGAAGAGTA CAGCGAGACC
    GACATCCTGC AGATGATTGG GAGAGCTGGC AGACCCCAGT TTGACACTAC AGCTACTGCA
    GTCATTATGA CTCGACTAAG TACAAGAGAG AAGTACGTGC AGATGCTAGC TTGTAACGAC
    ACTGTAGAGA GCAGTTTGCA CAGACATCTT ATTGAACATT TAAATGCAGA AATAGTGCTG
    CATACCATCA CAGACGTGAA CATTGCTCTG GACTGGATCC GATCAACAAT GCTGTATATC
    AGAGCTCTGA AAAACCCATC TCACTATGGT TTTTCATCTG GATTGAACAA AGATGGGATT
    GAAGCAAAAT TACAAGAATT ATGTTTGAAG AATCTGAAAG ATTTATCATC ACTAGACCTG
    ATAAAGATGG ATGAAGATGT TAATTTTAAA CCAACAGAGG CAGGAAGACT AATGGCTTGG
    TATTATATTA CATTTGAGAC AGTCAAGAAA TTTTGTGCAA TCAGTGGAAA AGAAACTTTA
    TTGGATTTAA TTTCAATGAT ATCGAGCTGC AATGAGTTTC TAGATGTGCA GCTGAGGATA
    AGTGAAAAGA GAATACTGAA CACCTTAAAC AAAGACCCAA ACCGGATAAC TATCAGATTT
    CCAATGGCAG AAAGAATTAA AACAAGAGAA ATGAAAGTAA ACTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCTATACA AGATTTTGCT TTGACACAAG ATACAGTGAA GATTTTCAGA
    AATGGCTCAC GAATTGCAAG ATGGTTGTCA GATTTTGTGG CTGCTCAGGA AAAGAAGTTT
    GCTGTGCTTT TAAACAGTGT GATTTTAACT AAATGTTTTA AGTGTAAACT CTGGGAAAAC
    TCTAAACATG TATCAAAACA GCTGGATAAA ATTGGCATAT CATTGTCAAA TACCATGGTA
    AATGCAGGCT TAACTTCTTT TAAAAAAATA GAAGAAGCAA ATGCAAGAGA ACTTGAACTG
    ATTTTAAATA GACACCCACC ATTTGGGACC CAGATAAAAG AAGCTGTGGC GCATCTGCCG
    AAGTATGAGC TTGAGGTGGA GCAGATTGCA AGATACAGTG ATATCAAGGC AGAAATACTG
    GTGACCATTA TATTAAGAAA CTTTGAGCAG CTGCAAACTA AAAGAACAGC ACCAGACTTC
    CACTATGCCA CCTTAATCAT AGGTGATGCA GATAACCAAG TCGTTTTTAA GCACAAAATT
    ATGGATTCTG TCTTGTTAAA AAGTGGAAAT TGGGTTAAAA AAATTGATGT GAAGAGAGCT
    CTTATATCAG AAGATCTCAG CATAAATCTA ATTAGTTCTG ATTATGTTGG TCTTGATATT
    CACCAGAAAT TTACAGTCTT TTATTTCGGA CCCAGGAAGT TTGTAAATGA AACAGCTATG
    GAAAGAAGCT CAGAAACAGA TATTTCTCAT TCTGACTACT CAGGTAGAGC CACTGCAACA
    GGATCCAGTA AAGGAATGGC TACCTGCAAA AAACCTGGGA ACAGAGAATG CCATCATCAC
    TGTAAAAATA AACACGCATG TGGACATGAC TGCTGTAAAA TTGGAGTTGC ACAAAAGCCG
    GAAGTTAAAG AGTCAGCAAT GTCTTCATAT CTGTCTGACT TAAAAAGCAG AGATGCTGTT
    TCTTCTCTTC CTCTAGCCAA GCGACTCAAG ATACAGATGA ACAAATCTCA AAATGTGGAC
    CTTAAAGAGT TTGGTTTTAC TCCAAGGCCT TCCCTTTCCA GTATATCAAG GTCAGAATAT
    TTAAATACCC CTGAATTGTC AATATTAGAA CAGAGGAATC AGCATGAAAT CTATGGAAAA
    GTTCAACAGG GACCATCTGA ATATCGTGAC AAAGATTTTC CTAGATGGGA CTATTGTACA
    ACTTCAAGTG AAAAGACCCC TCAAACGGTG ATGTTCAGCA GGCATGCTAA TATACCTGGA
    AACCACAGAG TTGCAGATGA AGACAAAAGC AAGTCTTCAG AAGTTCTGGG TGTGAACTTA
    GAGCTGGGAA ATGAAGTCTG GGATGACTTT GATGATGAGA GCTTAATAGA AGTTATGAGC
    CTTTCAGCTG ATGCTGAGAA GATGGCTGCA TCAGGATTTG GAGACACTCG TGATTCAAGT
    CTCGGCGGAA GTAAGCTACC CTTCCAAAAG TCAAGCAGCA GATTCCAAAG AGACAACTCA
    AACAGTTTTG CTTCGTCGCC TGGGAAGCCA GATGCTTATT TGCGAGATTG TTCCAGGTCC
    AGCTTCGGGC TGTCCTCTGT GGCAGAGATC CCTCAGCGGG CTGAGAATGC AAGTCTTGCC
    AATTTACAAG AGAGGAGACC GCTGACTCTC TCACCAGTGA TTGAGAGGAT GTGCTTTGCC
    CACTCTAAGA AAACACCACA GTCTCCCAAG TTTAAGGAAG TGGATATTTT TATTGGAAAC
    AGTGGCTCTA AAAAGGAAAT TGATCTTAGT AAGTATTATC CTGATGATGC AGCTGAAGAA
    ATGAAGGCTC TTCTGGGAAT ATTTAATGGC ATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110451.1
    CDS288..4586
    Translation

    Target ORF information:

    RefSeq Version XM_030254591.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GCCCAGTGAA ACTTACCCAA ACGAAAATAA GCAAAGAGAA TGCATGGACG
    TGTAGCAATA GTAAGCAGAA ACGTCAGTAT TCTACAAACA AATTCAAAGC AAATGATGCT
    TTTTCTGCTT CTGGAATTGG AAAAGACATA TTCAAAGCAC CATCATTTCC TGCTGCATCT
    CAGCCTCATG ATATTCAAGG AATAACCCCA AATGGCTTAG GTTCTTTGAA AGCTGTCACG
    GAAATCCCGG CAAAATTTAG AAATATATTC AAAGAATTCC CTTACTTCAA CTATATACAA
    TCCAAAGCCT TTGATGATCT TCTTTACACA GATAGGAATT TTGTGATTTG TGCCCCCACT
    GGTTCTGGGA AAACTGTAGT GTTTGAGCTA GCGATAACAA GATTGTTAAT GGAAGTACCA
    TTGCCATGGC TAAACATGAA GATTGTTTAC ATGGCACCAA TAAAAGCCCT TTGTAGTCAG
    CGGTTTGATG ACTGGAAAGA AAAGTTTGGA CCAGTAGGCT TGAATTGTAA AGAACTTACT
    GGTGATACAG TAATGGATGA CCTGTTTGAG ATTCAGCATG CAAATATAAT TATAACAACT
    CCAGAGAAGT GGGACAGTGT GACTAGGAAG TGGAGAGACA ACTCTTTTAT CCAACTGGTT
    CGACTGTTTC TCATTGATGA GGTGCATGTT ATTAAAGATG AAAATCGTGG TCCAACTCTT
    GAAGTTGTAG TTAGCAGAAT GAAAACTGTA CAGTCTTTAT CTAGGGATTT AGAAAGTGCC
    AGCCCTGTTC CAGTGAGATT TGTAGCTGTG TCTGCAACAA TTCCCAATGC TGAGGATATC
    GCAGAATGGC TTTCAGATGG TGAGAGACCT GCTGTATGTC TCAAAATGGA TGAGAGCCAT
    AGACCTGTGA AACTTCAGAA AGTAGTCCTG GGTTTTCCCT GTAGTAGTAG CCAAACTGAA
    TTTAAGTTTG ATTTGGCCCT CAACTATAAA GTGTACAGTG TTATTCGAAC ATACTCCGAT
    CAGAAGCCCA CACTTGTGTT TTGTTCAACA AGGAAAGGTG TGCAGCAGGC TGCTTCTGTT
    CTTGTGAAGG ATGCTAAGTT TATCATCTCT GTGGAGCAGA AACTACGGTT GCAGAAGTCT
    GCATATTCTA TAAGGGATTC AAAGCTGAAA GATACCTTGG TATATGGTGT TGGTTACCAT
    CATGCTGGAA TGGAGCTGTC AGATAGAAAA TTGGTTGAAG GATTGTTTAC CTCTGGAGAT
    CTACCAGTTC TTTTCACTAC CAGTACTCTA GCTATGGGCA TGAACATGCC GGCTCACCTG
    GTTGTTATAA AGTCTACCAT GCACTACAGT GGAGGTGTGT TTGAAGAGTA CAGCGAGACC
    GACATCCTGC AGATGATTGG GAGAGCTGGC AGACCCCAGT TTGACACTAC AGCTACTGCA
    GTCATTATGA CTCGACTAAG TACAAGAGAG AAGTACGTGC AGATGCTAGC TTGTAACGAC
    ACTGTAGAGA GCAGTTTGCA CAGACATCTT ATTGAACATT TAAATGCAGA AATAGTGCTG
    CATACCATCA CAGACGTGAA CATTGCTCTG GACTGGATCC GATCAACAAT GCTGTATATC
    AGAGCTCTGA AAAACCCATC TCACTATGGT TTTTCATCTG GATTGAACAA AGATGGGATT
    GAAGCAAAAT TACAAGAATT ATGTTTGAAG AATCTGAAAG ATTTATCATC ACTAGACCTG
    ATAAAGATGG ATGAAGATGT TAATTTTAAA CCAACAGAGG CAGGAAGACT AATGGCTTGG
    TATTATATTA CATTTGAGAC AGTCAAGAAA TTTTGTGCAA TCAGTGGAAA AGAAACTTTA
    TTGGATTTAA TTTCAATGAT ATCGAGCTGC AATGAGTTTC TAGATGTGCA GCTGAGGATA
    AGTGAAAAGA GAATACTGAA CACCTTAAAC AAAGACCCAA ACCGGATAAC TATCAGATTT
    CCAATGGCAG AAAGAATTAA AACAAGAGAA ATGAAAGTAA ACTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCTATACA AGATTTTGCT TTGACACAAG ATACAGTGAA GATTTTCAGA
    AATGGCTCAC GAATTGCAAG ATGGTTGTCA GATTTTGTGG CTGCTCAGGA AAAGAAGTTT
    GCTGTGCTTT TAAACAGTGT GATTTTAACT AAATGTTTTA AGTGTAAACT CTGGGAAAAC
    TCTAAACATG TATCAAAACA GCTGGATAAA ATTGGCATAT CATTGTCAAA TACCATGGTA
    AATGCAGGCT TAACTTCTTT TAAAAAAATA GAAGAAGCAA ATGCAAGAGA ACTTGAACTG
    ATTTTAAATA GACACCCACC ATTTGGGACC CAGATAAAAG AAGCTGTGGC GCATCTGCCG
    AAGTATGAGC TTGAGGTGGA GCAGATTGCA AGATACAGTG ATATCAAGGC AGAAATACTG
    GTGACCATTA TATTAAGAAA CTTTGAGCAG CTGCAAACTA AAAGAACAGC ACCAGACTTC
    CACTATGCCA CCTTAATCAT AGGTGATGCA GATAACCAAG TCGTTTTTAA GCACAAAATT
    ATGGATTCTG TCTTGTTAAA AAGTGGAAAT TGGGTTAAAA AAATTGATGT GAAGAGAGCT
    CTTATATCAG AAGATCTCAG CATAAATCTA ATTAGTTCTG ATTATGTTGG TCTTGATATT
    CACCAGAAAT TTACAGTCTT TTATTTCGGA CCCAGGAAGT TTGTAAATGA AACAGCTATG
    GAAAGAAGCT CAGAAACAGA TATTTCTCAT TCTGACTACT CAGGTAGAGC CACTGCAACA
    GGATCCAGTA AAGGAATGGC TACCTGCAAA AAACCTGGGA ACAGAGAATG CCATCATCAC
    TGTAAAAATA AACACGCATG TGGACATGAC TGCTGTAAAA TTGGAGTTGC ACAAAAGCCG
    GAAGTTAAAG AGTCAGCAAT GTCTTCATAT CTGTCTGACT TAAAAAGCAG AGATGCTGTT
    TCTTCTCTTC CTCTAGCCAA GCGACTCAAG ATACAGATGA ACAAATCTCA AAATGTGGAC
    CTTAAAGAGT TTGGTTTTAC TCCAAGGCCT TCCCTTTCCA GTATATCAAG GTCAGAATAT
    TTAAATACCC CTGAATTGTC AATATTAGAA CAGAGGAATC AGCATGAAAT CTATGGAAAA
    GTTCAACAGG GACCATCTGA ATATCGTGAC AAAGATTTTC CTAGATGGGA CTATTGTACA
    ACTTCAAGTG AAAAGACCCC TCAAACGGTG ATGTTCAGCA GGCATGCTAA TATACCTGGA
    AACCACAGAG TTGCAGATGA AGACAAAAGC AAGTCTTCAG AAGTTCTGGG TGTGAACTTA
    GAGCTGGGAA ATGAAGTCTG GGATGACTTT GATGATGAGA GCTTAATAGA AGTTATGAGC
    CTTTCAGCTG ATGCTGAGAA GATGGCTGCA TCAGGATTTG GAGACACTCG TGATTCAAGT
    CTCGGCGGAA GTAAGCTACC CTTCCAAAAG TCAAGCAGCA GATTCCAAAG AGACAACTCA
    AACAGTTTTG CTTCGTCGCC TGGGAAGCCA GATGCTTATT TGCGAGATTG TTCCAGGTCC
    AGCTTCGGGC TGTCCTCTGT GGCAGAGATC CCTCAGCGGG CTGAGAATGC AAGTCTTGCC
    AATTTACAAG AGAGGAGACC GCTGACTCTC TCACCAGTGA TTGAGAGGAT GTGCTTTGCC
    CACTCTAAGA AAACACCACA GTCTCCCAAG TTTAAGGAAG TGGATATTTT TATTGGAAAC
    AGTGGCTCTA AAAAGGAAAT TGATCTTAGT AAGTATTATC CTGATGATGC AGCTGAAGAA
    ATGAAGGCTC TTCTGGGAAT ATTTAATGGC ATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88943
    Clone ID Related Accession (Same CDS sequence) XM_030254591.1 , XM_030254592.1
    Accession Version XM_030254592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GCCCAGTGAA ACTTACCCAA ACGAAAATAA GCAAAGAGAA TGCATGGACG
    TGTAGCAATA GTAAGCAGAA ACGTCAGTAT TCTACAAACA AATTCAAAGC AAATGATGCT
    TTTTCTGCTT CTGGAATTGG AAAAGACATA TTCAAAGCAC CATCATTTCC TGCTGCATCT
    CAGCCTCATG ATATTCAAGG AATAACCCCA AATGGCTTAG GTTCTTTGAA AGCTGTCACG
    GAAATCCCGG CAAAATTTAG AAATATATTC AAAGAATTCC CTTACTTCAA CTATATACAA
    TCCAAAGCCT TTGATGATCT TCTTTACACA GATAGGAATT TTGTGATTTG TGCCCCCACT
    GGTTCTGGGA AAACTGTAGT GTTTGAGCTA GCGATAACAA GATTGTTAAT GGAAGTACCA
    TTGCCATGGC TAAACATGAA GATTGTTTAC ATGGCACCAA TAAAAGCCCT TTGTAGTCAG
    CGGTTTGATG ACTGGAAAGA AAAGTTTGGA CCAGTAGGCT TGAATTGTAA AGAACTTACT
    GGTGATACAG TAATGGATGA CCTGTTTGAG ATTCAGCATG CAAATATAAT TATAACAACT
    CCAGAGAAGT GGGACAGTGT GACTAGGAAG TGGAGAGACA ACTCTTTTAT CCAACTGGTT
    CGACTGTTTC TCATTGATGA GGTGCATGTT ATTAAAGATG AAAATCGTGG TCCAACTCTT
    GAAGTTGTAG TTAGCAGAAT GAAAACTGTA CAGTCTTTAT CTAGGGATTT AGAAAGTGCC
    AGCCCTGTTC CAGTGAGATT TGTAGCTGTG TCTGCAACAA TTCCCAATGC TGAGGATATC
    GCAGAATGGC TTTCAGATGG TGAGAGACCT GCTGTATGTC TCAAAATGGA TGAGAGCCAT
    AGACCTGTGA AACTTCAGAA AGTAGTCCTG GGTTTTCCCT GTAGTAGTAG CCAAACTGAA
    TTTAAGTTTG ATTTGGCCCT CAACTATAAA GTGTACAGTG TTATTCGAAC ATACTCCGAT
    CAGAAGCCCA CACTTGTGTT TTGTTCAACA AGGAAAGGTG TGCAGCAGGC TGCTTCTGTT
    CTTGTGAAGG ATGCTAAGTT TATCATCTCT GTGGAGCAGA AACTACGGTT GCAGAAGTCT
    GCATATTCTA TAAGGGATTC AAAGCTGAAA GATACCTTGG TATATGGTGT TGGTTACCAT
    CATGCTGGAA TGGAGCTGTC AGATAGAAAA TTGGTTGAAG GATTGTTTAC CTCTGGAGAT
    CTACCAGTTC TTTTCACTAC CAGTACTCTA GCTATGGGCA TGAACATGCC GGCTCACCTG
    GTTGTTATAA AGTCTACCAT GCACTACAGT GGAGGTGTGT TTGAAGAGTA CAGCGAGACC
    GACATCCTGC AGATGATTGG GAGAGCTGGC AGACCCCAGT TTGACACTAC AGCTACTGCA
    GTCATTATGA CTCGACTAAG TACAAGAGAG AAGTACGTGC AGATGCTAGC TTGTAACGAC
    ACTGTAGAGA GCAGTTTGCA CAGACATCTT ATTGAACATT TAAATGCAGA AATAGTGCTG
    CATACCATCA CAGACGTGAA CATTGCTCTG GACTGGATCC GATCAACAAT GCTGTATATC
    AGAGCTCTGA AAAACCCATC TCACTATGGT TTTTCATCTG GATTGAACAA AGATGGGATT
    GAAGCAAAAT TACAAGAATT ATGTTTGAAG AATCTGAAAG ATTTATCATC ACTAGACCTG
    ATAAAGATGG ATGAAGATGT TAATTTTAAA CCAACAGAGG CAGGAAGACT AATGGCTTGG
    TATTATATTA CATTTGAGAC AGTCAAGAAA TTTTGTGCAA TCAGTGGAAA AGAAACTTTA
    TTGGATTTAA TTTCAATGAT ATCGAGCTGC AATGAGTTTC TAGATGTGCA GCTGAGGATA
    AGTGAAAAGA GAATACTGAA CACCTTAAAC AAAGACCCAA ACCGGATAAC TATCAGATTT
    CCAATGGCAG AAAGAATTAA AACAAGAGAA ATGAAAGTAA ACTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCTATACA AGATTTTGCT TTGACACAAG ATACAGTGAA GATTTTCAGA
    AATGGCTCAC GAATTGCAAG ATGGTTGTCA GATTTTGTGG CTGCTCAGGA AAAGAAGTTT
    GCTGTGCTTT TAAACAGTGT GATTTTAACT AAATGTTTTA AGTGTAAACT CTGGGAAAAC
    TCTAAACATG TATCAAAACA GCTGGATAAA ATTGGCATAT CATTGTCAAA TACCATGGTA
    AATGCAGGCT TAACTTCTTT TAAAAAAATA GAAGAAGCAA ATGCAAGAGA ACTTGAACTG
    ATTTTAAATA GACACCCACC ATTTGGGACC CAGATAAAAG AAGCTGTGGC GCATCTGCCG
    AAGTATGAGC TTGAGGTGGA GCAGATTGCA AGATACAGTG ATATCAAGGC AGAAATACTG
    GTGACCATTA TATTAAGAAA CTTTGAGCAG CTGCAAACTA AAAGAACAGC ACCAGACTTC
    CACTATGCCA CCTTAATCAT AGGTGATGCA GATAACCAAG TCGTTTTTAA GCACAAAATT
    ATGGATTCTG TCTTGTTAAA AAGTGGAAAT TGGGTTAAAA AAATTGATGT GAAGAGAGCT
    CTTATATCAG AAGATCTCAG CATAAATCTA ATTAGTTCTG ATTATGTTGG TCTTGATATT
    CACCAGAAAT TTACAGTCTT TTATTTCGGA CCCAGGAAGT TTGTAAATGA AACAGCTATG
    GAAAGAAGCT CAGAAACAGA TATTTCTCAT TCTGACTACT CAGGTAGAGC CACTGCAACA
    GGATCCAGTA AAGGAATGGC TACCTGCAAA AAACCTGGGA ACAGAGAATG CCATCATCAC
    TGTAAAAATA AACACGCATG TGGACATGAC TGCTGTAAAA TTGGAGTTGC ACAAAAGCCG
    GAAGTTAAAG AGTCAGCAAT GTCTTCATAT CTGTCTGACT TAAAAAGCAG AGATGCTGTT
    TCTTCTCTTC CTCTAGCCAA GCGACTCAAG ATACAGATGA ACAAATCTCA AAATGTGGAC
    CTTAAAGAGT TTGGTTTTAC TCCAAGGCCT TCCCTTTCCA GTATATCAAG GTCAGAATAT
    TTAAATACCC CTGAATTGTC AATATTAGAA CAGAGGAATC AGCATGAAAT CTATGGAAAA
    GTTCAACAGG GACCATCTGA ATATCGTGAC AAAGATTTTC CTAGATGGGA CTATTGTACA
    ACTTCAAGTG AAAAGACCCC TCAAACGGTG ATGTTCAGCA GGCATGCTAA TATACCTGGA
    AACCACAGAG TTGCAGATGA AGACAAAAGC AAGTCTTCAG AAGTTCTGGG TGTGAACTTA
    GAGCTGGGAA ATGAAGTCTG GGATGACTTT GATGATGAGA GCTTAATAGA AGTTATGAGC
    CTTTCAGCTG ATGCTGAGAA GATGGCTGCA TCAGGATTTG GAGACACTCG TGATTCAAGT
    CTCGGCGGAA GTAAGCTACC CTTCCAAAAG TCAAGCAGCA GATTCCAAAG AGACAACTCA
    AACAGTTTTG CTTCGTCGCC TGGGAAGCCA GATGCTTATT TGCGAGATTG TTCCAGGTCC
    AGCTTCGGGC TGTCCTCTGT GGCAGAGATC CCTCAGCGGG CTGAGAATGC AAGTCTTGCC
    AATTTACAAG AGAGGAGACC GCTGACTCTC TCACCAGTGA TTGAGAGGAT GTGCTTTGCC
    CACTCTAAGA AAACACCACA GTCTCCCAAG TTTAAGGAAG TGGATATTTT TATTGGAAAC
    AGTGGCTCTA AAAAGGAAAT TGATCTTAGT AAGTATTATC CTGATGATGC AGCTGAAGAA
    ATGAAGGCTC TTCTGGGAAT ATTTAATGGC ATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110452.1
    CDS459..4757
    Translation

    Target ORF information:

    RefSeq Version XM_030254592.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAAACTGA AAACAAGAAC GACGCTGCAA CAGGATCCTT CTGCACACGT TTATTGGGAG 
    AGCTTGATTG CAGAGGCGAA AAGACTTCGA GCCCAGAACT GGTGCTGCTT TTATAGGCCT
    AGGAGAGGGA AAGGAAAGAT GCCAAGAATG TTAACATCAA ACTTGAAGAT CATTAATGAA
    GACACAAATT GTATTTCACC AACACAAAAA ATCCATTTTT CCTATAATGT ACATGAACAG
    GATTACCTAA ATTTAGGAGG ATCAAATAAC AATGACATGT CCCATGTAGC TGGGAAGCTG
    ATGTATGGTT CTTCTCAGAA ATATAAAAAT CACATGGGTG CTAAGAGTCC ATCTGCAAGG
    AGTAGTCCTG GTGATACAAA ATTACATGAT GTGGCAGAAG ACAGACAGGG CACATCAGCA
    TTCAAAAAAA GCCCAGTGAA ACTTACCCAA ACGAAAATAA GCAAAGAGAA TGCATGGACG
    TGTAGCAATA GTAAGCAGAA ACGTCAGTAT TCTACAAACA AATTCAAAGC AAATGATGCT
    TTTTCTGCTT CTGGAATTGG AAAAGACATA TTCAAAGCAC CATCATTTCC TGCTGCATCT
    CAGCCTCATG ATATTCAAGG AATAACCCCA AATGGCTTAG GTTCTTTGAA AGCTGTCACG
    GAAATCCCGG CAAAATTTAG AAATATATTC AAAGAATTCC CTTACTTCAA CTATATACAA
    TCCAAAGCCT TTGATGATCT TCTTTACACA GATAGGAATT TTGTGATTTG TGCCCCCACT
    GGTTCTGGGA AAACTGTAGT GTTTGAGCTA GCGATAACAA GATTGTTAAT GGAAGTACCA
    TTGCCATGGC TAAACATGAA GATTGTTTAC ATGGCACCAA TAAAAGCCCT TTGTAGTCAG
    CGGTTTGATG ACTGGAAAGA AAAGTTTGGA CCAGTAGGCT TGAATTGTAA AGAACTTACT
    GGTGATACAG TAATGGATGA CCTGTTTGAG ATTCAGCATG CAAATATAAT TATAACAACT
    CCAGAGAAGT GGGACAGTGT GACTAGGAAG TGGAGAGACA ACTCTTTTAT CCAACTGGTT
    CGACTGTTTC TCATTGATGA GGTGCATGTT ATTAAAGATG AAAATCGTGG TCCAACTCTT
    GAAGTTGTAG TTAGCAGAAT GAAAACTGTA CAGTCTTTAT CTAGGGATTT AGAAAGTGCC
    AGCCCTGTTC CAGTGAGATT TGTAGCTGTG TCTGCAACAA TTCCCAATGC TGAGGATATC
    GCAGAATGGC TTTCAGATGG TGAGAGACCT GCTGTATGTC TCAAAATGGA TGAGAGCCAT
    AGACCTGTGA AACTTCAGAA AGTAGTCCTG GGTTTTCCCT GTAGTAGTAG CCAAACTGAA
    TTTAAGTTTG ATTTGGCCCT CAACTATAAA GTGTACAGTG TTATTCGAAC ATACTCCGAT
    CAGAAGCCCA CACTTGTGTT TTGTTCAACA AGGAAAGGTG TGCAGCAGGC TGCTTCTGTT
    CTTGTGAAGG ATGCTAAGTT TATCATCTCT GTGGAGCAGA AACTACGGTT GCAGAAGTCT
    GCATATTCTA TAAGGGATTC AAAGCTGAAA GATACCTTGG TATATGGTGT TGGTTACCAT
    CATGCTGGAA TGGAGCTGTC AGATAGAAAA TTGGTTGAAG GATTGTTTAC CTCTGGAGAT
    CTACCAGTTC TTTTCACTAC CAGTACTCTA GCTATGGGCA TGAACATGCC GGCTCACCTG
    GTTGTTATAA AGTCTACCAT GCACTACAGT GGAGGTGTGT TTGAAGAGTA CAGCGAGACC
    GACATCCTGC AGATGATTGG GAGAGCTGGC AGACCCCAGT TTGACACTAC AGCTACTGCA
    GTCATTATGA CTCGACTAAG TACAAGAGAG AAGTACGTGC AGATGCTAGC TTGTAACGAC
    ACTGTAGAGA GCAGTTTGCA CAGACATCTT ATTGAACATT TAAATGCAGA AATAGTGCTG
    CATACCATCA CAGACGTGAA CATTGCTCTG GACTGGATCC GATCAACAAT GCTGTATATC
    AGAGCTCTGA AAAACCCATC TCACTATGGT TTTTCATCTG GATTGAACAA AGATGGGATT
    GAAGCAAAAT TACAAGAATT ATGTTTGAAG AATCTGAAAG ATTTATCATC ACTAGACCTG
    ATAAAGATGG ATGAAGATGT TAATTTTAAA CCAACAGAGG CAGGAAGACT AATGGCTTGG
    TATTATATTA CATTTGAGAC AGTCAAGAAA TTTTGTGCAA TCAGTGGAAA AGAAACTTTA
    TTGGATTTAA TTTCAATGAT ATCGAGCTGC AATGAGTTTC TAGATGTGCA GCTGAGGATA
    AGTGAAAAGA GAATACTGAA CACCTTAAAC AAAGACCCAA ACCGGATAAC TATCAGATTT
    CCAATGGCAG AAAGAATTAA AACAAGAGAA ATGAAAGTAA ACTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCTATACA AGATTTTGCT TTGACACAAG ATACAGTGAA GATTTTCAGA
    AATGGCTCAC GAATTGCAAG ATGGTTGTCA GATTTTGTGG CTGCTCAGGA AAAGAAGTTT
    GCTGTGCTTT TAAACAGTGT GATTTTAACT AAATGTTTTA AGTGTAAACT CTGGGAAAAC
    TCTAAACATG TATCAAAACA GCTGGATAAA ATTGGCATAT CATTGTCAAA TACCATGGTA
    AATGCAGGCT TAACTTCTTT TAAAAAAATA GAAGAAGCAA ATGCAAGAGA ACTTGAACTG
    ATTTTAAATA GACACCCACC ATTTGGGACC CAGATAAAAG AAGCTGTGGC GCATCTGCCG
    AAGTATGAGC TTGAGGTGGA GCAGATTGCA AGATACAGTG ATATCAAGGC AGAAATACTG
    GTGACCATTA TATTAAGAAA CTTTGAGCAG CTGCAAACTA AAAGAACAGC ACCAGACTTC
    CACTATGCCA CCTTAATCAT AGGTGATGCA GATAACCAAG TCGTTTTTAA GCACAAAATT
    ATGGATTCTG TCTTGTTAAA AAGTGGAAAT TGGGTTAAAA AAATTGATGT GAAGAGAGCT
    CTTATATCAG AAGATCTCAG CATAAATCTA ATTAGTTCTG ATTATGTTGG TCTTGATATT
    CACCAGAAAT TTACAGTCTT TTATTTCGGA CCCAGGAAGT TTGTAAATGA AACAGCTATG
    GAAAGAAGCT CAGAAACAGA TATTTCTCAT TCTGACTACT CAGGTAGAGC CACTGCAACA
    GGATCCAGTA AAGGAATGGC TACCTGCAAA AAACCTGGGA ACAGAGAATG CCATCATCAC
    TGTAAAAATA AACACGCATG TGGACATGAC TGCTGTAAAA TTGGAGTTGC ACAAAAGCCG
    GAAGTTAAAG AGTCAGCAAT GTCTTCATAT CTGTCTGACT TAAAAAGCAG AGATGCTGTT
    TCTTCTCTTC CTCTAGCCAA GCGACTCAAG ATACAGATGA ACAAATCTCA AAATGTGGAC
    CTTAAAGAGT TTGGTTTTAC TCCAAGGCCT TCCCTTTCCA GTATATCAAG GTCAGAATAT
    TTAAATACCC CTGAATTGTC AATATTAGAA CAGAGGAATC AGCATGAAAT CTATGGAAAA
    GTTCAACAGG GACCATCTGA ATATCGTGAC AAAGATTTTC CTAGATGGGA CTATTGTACA
    ACTTCAAGTG AAAAGACCCC TCAAACGGTG ATGTTCAGCA GGCATGCTAA TATACCTGGA
    AACCACAGAG TTGCAGATGA AGACAAAAGC AAGTCTTCAG AAGTTCTGGG TGTGAACTTA
    GAGCTGGGAA ATGAAGTCTG GGATGACTTT GATGATGAGA GCTTAATAGA AGTTATGAGC
    CTTTCAGCTG ATGCTGAGAA GATGGCTGCA TCAGGATTTG GAGACACTCG TGATTCAAGT
    CTCGGCGGAA GTAAGCTACC CTTCCAAAAG TCAAGCAGCA GATTCCAAAG AGACAACTCA
    AACAGTTTTG CTTCGTCGCC TGGGAAGCCA GATGCTTATT TGCGAGATTG TTCCAGGTCC
    AGCTTCGGGC TGTCCTCTGT GGCAGAGATC CCTCAGCGGG CTGAGAATGC AAGTCTTGCC
    AATTTACAAG AGAGGAGACC GCTGACTCTC TCACCAGTGA TTGAGAGGAT GTGCTTTGCC
    CACTCTAAGA AAACACCACA GTCTCCCAAG TTTAAGGAAG TGGATATTTT TATTGGAAAC
    AGTGGCTCTA AAAAGGAAAT TGATCTTAGT AAGTATTATC CTGATGATGC AGCTGAAGAA
    ATGAAGGCTC TTCTGGGAAT ATTTAATGGC ATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88944
    Clone ID Related Accession (Same CDS sequence) XM_030254595.1
    Accession Version XM_030254595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCCAAGAA TGTTAACATC AAACTTGAAG ATCATTAATG AAGACACAAA TTGTATTTCA 
    CCAACACAAA AAATCCATTT TTCCTATAAT GTACATGAAC AGGATTACCT AAATTTAGGA
    GGATCAAATA ACAATGACAT GTCCCATGTA GCTGGGAAGC TGATGTATGG TTCTTCTCAG
    AAATATAAAA ATCACATGGG TGCTAAGAGT CCATCTGCAA GGAGTAGTCC TGGTGATACA
    AAATTACATG ATGTGGCAGA AGACAGACAG GGCACATCAG CATTCAAAAA AAGCCCAGTG
    AAACTTACCC AAACGAAAAT AAGCAAAGAG AATGCATGGA CGTGTAGCAA TAGTAAGCAG
    AAACGTCAGT ATTCTACAAA CAAATTCAAA GCAAATGATG CTTTTTCTGC TTCTGGAATT
    GGAAAAGACA TATTCAAAGC ACCATCATTT CCTGCTGCAT CTCAGCCTCA TGATATTCAA
    GGAATAACCC CAAATGGCTT AGGTTCTTTG AAAGCTGTCA CGGAAATCCC GGCAAAATTT
    AGAAATATAT TCAAAGAATT CCCTTACTTC AACTATATAC AATCCAAAGC CTTTGATGAT
    CTTCTTTACA CAGATAGGAA TTTTGTGATT TGTGCCCCCA CTGGTTCTGG GAAAACTGTA
    GTGTTTGAGC TAGCGATAAC AAGATTGTTA ATGGAAGTAC CATTGCCATG GCTAAACATG
    AAGATTGTTT ACATGGCACC AATAAAAGCC CTTTGTAGTC AGCGGTTTGA TGACTGGAAA
    GAAAAGTTTG GACCAGTAGG CTTGAATTGT AAAGAACTTA CTGGTGATAC AGTAATGGAT
    GACCTGTTTG AGATTCAGCA TGCAAATATA ATTATAACAA CTCCAGAGAA GTGGGACAGT
    GTGACTAGGA AGTGGAGAGA CAACTCTTTT ATCCAACTGG TTCGACTGTT TCTCATTGAT
    GAGGTGCATG TTATTAAAGA TGAAAATCGT GGTCCAACTC TTGAAGTTGT AGTTAGCAGA
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110455.1
    CDS520..4680
    Translation

    Target ORF information:

    RefSeq Version XM_030254595.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCCAAGAA TGTTAACATC AAACTTGAAG ATCATTAATG AAGACACAAA TTGTATTTCA 
    CCAACACAAA AAATCCATTT TTCCTATAAT GTACATGAAC AGGATTACCT AAATTTAGGA
    GGATCAAATA ACAATGACAT GTCCCATGTA GCTGGGAAGC TGATGTATGG TTCTTCTCAG
    AAATATAAAA ATCACATGGG TGCTAAGAGT CCATCTGCAA GGAGTAGTCC TGGTGATACA
    AAATTACATG ATGTGGCAGA AGACAGACAG GGCACATCAG CATTCAAAAA AAGCCCAGTG
    AAACTTACCC AAACGAAAAT AAGCAAAGAG AATGCATGGA CGTGTAGCAA TAGTAAGCAG
    AAACGTCAGT ATTCTACAAA CAAATTCAAA GCAAATGATG CTTTTTCTGC TTCTGGAATT
    GGAAAAGACA TATTCAAAGC ACCATCATTT CCTGCTGCAT CTCAGCCTCA TGATATTCAA
    GGAATAACCC CAAATGGCTT AGGTTCTTTG AAAGCTGTCA CGGAAATCCC GGCAAAATTT
    AGAAATATAT TCAAAGAATT CCCTTACTTC AACTATATAC AATCCAAAGC CTTTGATGAT
    CTTCTTTACA CAGATAGGAA TTTTGTGATT TGTGCCCCCA CTGGTTCTGG GAAAACTGTA
    GTGTTTGAGC TAGCGATAAC AAGATTGTTA ATGGAAGTAC CATTGCCATG GCTAAACATG
    AAGATTGTTT ACATGGCACC AATAAAAGCC CTTTGTAGTC AGCGGTTTGA TGACTGGAAA
    GAAAAGTTTG GACCAGTAGG CTTGAATTGT AAAGAACTTA CTGGTGATAC AGTAATGGAT
    GACCTGTTTG AGATTCAGCA TGCAAATATA ATTATAACAA CTCCAGAGAA GTGGGACAGT
    GTGACTAGGA AGTGGAGAGA CAACTCTTTT ATCCAACTGG TTCGACTGTT TCTCATTGAT
    GAGGTGCATG TTATTAAAGA TGAAAATCGT GGTCCAACTC TTGAAGTTGT AGTTAGCAGA
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88945
    Clone ID Related Accession (Same CDS sequence) XM_030254599.1 , XM_030254598.1 , XM_030254597.1 , XM_030254600.1
    Accession Version XM_030254599.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110459.1
    CDS1730..4870
    Translation

    Target ORF information:

    RefSeq Version XM_030254599.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88945
    Clone ID Related Accession (Same CDS sequence) XM_030254599.1 , XM_030254598.1 , XM_030254597.1 , XM_030254600.1
    Accession Version XM_030254598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110458.1
    CDS1459..4599
    Translation

    Target ORF information:

    RefSeq Version XM_030254598.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88945
    Clone ID Related Accession (Same CDS sequence) XM_030254599.1 , XM_030254598.1 , XM_030254597.1 , XM_030254600.1
    Accession Version XM_030254597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110457.1
    CDS1463..4603
    Translation

    Target ORF information:

    RefSeq Version XM_030254597.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88945
    Clone ID Related Accession (Same CDS sequence) XM_030254599.1 , XM_030254598.1 , XM_030254597.1 , XM_030254600.1
    Accession Version XM_030254600.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110460.1
    CDS1275..4415
    Translation

    Target ORF information:

    RefSeq Version XM_030254600.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254600.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAAACTG TACAGTCTTT ATCTAGGGAT TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA 
    TTTGTAGCTG TGTCTGCAAC AATTCCCAAT GCTGAGGATA TCGCAGAATG GCTTTCAGAT
    GGTGAGAGAC CTGCTGTATG TCTCAAAATG GATGAGAGCC ATAGACCTGT GAAACTTCAG
    AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT AGCCAAACTG AATTTAAGTT TGATTTGGCC
    CTCAACTATA AAGTGTACAG TGTTATTCGA ACATACTCCG ATCAGAAGCC CACACTTGTG
    TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG GCTGCTTCTG TTCTTGTGAA GGATGCTAAG
    TTTATCATCT CTGTGGAGCA GAAACTACGG TTGCAGAAGT CTGCATATTC TATAAGGGAT
    TCAAAGCTGA AAGATACCTT GGTATATGGT GTTGGTTACC ATCATGCTGG AATGGAGCTG
    TCAGATAGAA AATTGGTTGA AGGATTGTTT ACCTCTGGAG ATCTACCAGT TCTTTTCACT
    ACCAGTACTC TAGCTATGGG CATGAACATG CCGGCTCACC TGGTTGTTAT AAAGTCTACC
    ATGCACTACA GTGGAGGTGT GTTTGAAGAG TACAGCGAGA CCGACATCCT GCAGATGATT
    GGGAGAGCTG GCAGACCCCA GTTTGACACT ACAGCTACTG CAGTCATTAT GACTCGACTA
    AGTACAAGAG AGAAGTACGT GCAGATGCTA GCTTGTAACG ACACTGTAGA GAGCAGTTTG
    CACAGACATC TTATTGAACA TTTAAATGCA GAAATAGTGC TGCATACCAT CACAGACGTG
    AACATTGCTC TGGACTGGAT CCGATCAACA ATGCTGTATA TCAGAGCTCT GAAAAACCCA
    TCTCACTATG GTTTTTCATC TGGATTGAAC AAAGATGGGA TTGAAGCAAA ATTACAAGAA
    TTATGTTTGA AGAATCTGAA AGATTTATCA TCACTAGACC TGATAAAGAT GGATGAAGAT
    GTTAATTTTA AACCAACAGA GGCAGGAAGA CTAATGGCTT GGTATTATAT TACATTTGAG
    ACAGTCAAGA AATTTTGTGC AATCAGTGGA AAAGAAACTT TATTGGATTT AATTTCAATG
    ATATCGAGCT GCAATGAGTT TCTAGATGTG CAGCTGAGGA TAAGTGAAAA GAGAATACTG
    AACACCTTAA ACAAAGACCC AAACCGGATA ACTATCAGAT TTCCAATGGC AGAAAGAATT
    AAAACAAGAG AAATGAAAGT AAACTGTCTT ATTCAGGCTC AGCTAGGATG CATTCCTATA
    CAAGATTTTG CTTTGACACA AGATACAGTG AAGATTTTCA GAAATGGCTC ACGAATTGCA
    AGATGGTTGT CAGATTTTGT GGCTGCTCAG GAAAAGAAGT TTGCTGTGCT TTTAAACAGT
    GTGATTTTAA CTAAATGTTT TAAGTGTAAA CTCTGGGAAA ACTCTAAACA TGTATCAAAA
    CAGCTGGATA AAATTGGCAT ATCATTGTCA AATACCATGG TAAATGCAGG CTTAACTTCT
    TTTAAAAAAA TAGAAGAAGC AAATGCAAGA GAACTTGAAC TGATTTTAAA TAGACACCCA
    CCATTTGGGA CCCAGATAAA AGAAGCTGTG GCGCATCTGC CGAAGTATGA GCTTGAGGTG
    GAGCAGATTG CAAGATACAG TGATATCAAG GCAGAAATAC TGGTGACCAT TATATTAAGA
    AACTTTGAGC AGCTGCAAAC TAAAAGAACA GCACCAGACT TCCACTATGC CACCTTAATC
    ATAGGTGATG CAGATAACCA AGTCGTTTTT AAGCACAAAA TTATGGATTC TGTCTTGTTA
    AAAAGTGGAA ATTGGGTTAA AAAAATTGAT GTGAAGAGAG CTCTTATATC AGAAGATCTC
    AGCATAAATC TAATTAGTTC TGATTATGTT GGTCTTGATA TTCACCAGAA ATTTACAGTC
    TTTTATTTCG GACCCAGGAA GTTTGTAAAT GAAACAGCTA TGGAAAGAAG CTCAGAAACA
    GATATTTCTC ATTCTGACTA CTCAGGTAGA GCCACTGCAA CAGGATCCAG TAAAGGAATG
    GCTACCTGCA AAAAACCTGG GAACAGAGAA TGCCATCATC ACTGTAAAAA TAAACACGCA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAAAAGC CGGAAGTTAA AGAGTCAGCA
    ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC AGAGATGCTG TTTCTTCTCT TCCTCTAGCC
    AAGCGACTCA AGATACAGAT GAACAAATCT CAAAATGTGG ACCTTAAAGA GTTTGGTTTT
    ACTCCAAGGC CTTCCCTTTC CAGTATATCA AGGTCAGAAT ATTTAAATAC CCCTGAATTG
    TCAATATTAG AACAGAGGAA TCAGCATGAA ATCTATGGAA AAGTTCAACA GGGACCATCT
    GAATATCGTG ACAAAGATTT TCCTAGATGG GACTATTGTA CAACTTCAAG TGAAAAGACC
    CCTCAAACGG TGATGTTCAG CAGGCATGCT AATATACCTG GAAACCACAG AGTTGCAGAT
    GAAGACAAAA GCAAGTCTTC AGAAGTTCTG GGTGTGAACT TAGAGCTGGG AAATGAAGTC
    TGGGATGACT TTGATGATGA GAGCTTAATA GAAGTTATGA GCCTTTCAGC TGATGCTGAG
    AAGATGGCTG CATCAGGATT TGGAGACACT CGTGATTCAA GTCTCGGCGG AAGTAAGCTA
    CCCTTCCAAA AGTCAAGCAG CAGATTCCAA AGAGACAACT CAAACAGTTT TGCTTCGTCG
    CCTGGGAAGC CAGATGCTTA TTTGCGAGAT TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT
    GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT GCAAGTCTTG CCAATTTACA AGAGAGGAGA
    CCGCTGACTC TCTCACCAGT GATTGAGAGG ATGTGCTTTG CCCACTCTAA GAAAACACCA
    CAGTCTCCCA AGTTTAAGGA AGTGGATATT TTTATTGGAA ACAGTGGCTC TAAAAAGGAA
    ATTGATCTTA GTAAGTATTA TCCTGATGAT GCAGCTGAAG AAATGAAGGC TCTTCTGGGA
    ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74858
    Clone ID Related Accession (Same CDS sequence) XM_011249488.1 , XM_030254596.1
    Accession Version XM_011249488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC 
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247790.1
    CDS177..3647
    Translation

    Target ORF information:

    RefSeq Version XM_011249488.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC 
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35526
    Clone ID Related Accession (Same CDS sequence) XM_006535016.3 , XM_011249484.1 , XM_006535015.1 , XM_006535016.4
    Accession Version XM_006535016.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006535079.1
    CDS259..4689
    Translation

    Target ORF information:

    RefSeq Version XM_006535016.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006535016.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35526
    Clone ID Related Accession (Same CDS sequence) XM_006535016.3 , XM_011249484.1 , XM_006535015.1 , XM_006535016.4
    Accession Version XM_011249484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247786.1
    CDS211..4641
    Translation

    Target ORF information:

    RefSeq Version XM_011249484.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35526
    Clone ID Related Accession (Same CDS sequence) XM_006535016.3 , XM_011249484.1 , XM_006535015.1 , XM_006535016.4
    Accession Version XM_006535015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006535078.1
    CDS479..4909
    Translation

    Target ORF information:

    RefSeq Version XM_006535015.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006535015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGAC TGTCTAGAAC ATGTGACAGT GAACATGACT GTGACTATGC TGATGGAAGT
    TTAAACTTGA GTTCTCATAT CAGCCCAGTG AAACTTACCC AAACGAAAAT AAGCAAAGAG
    AATGCATGGA CGTGTAGCAA TAGTAAGCAG AAACGTCAGT ATTCTACAAA CAAATTCAAA
    GCAAATGATG CTTTTTCTGC TTCTGGAATT GGAAAAGACA TATTCAAAGC ACCATCATTT
    CCTGCTGCAT CTCAGCCTCA TGATATTCAA GGAATAACCC CAAATGGCTT AGGTTCTTTG
    AAAGCTGTCA CGGAAATCCC GGCAAAATTT AGAAATATAT TCAAAGAATT CCCTTACTTC
    AACTATATAC AATCCAAAGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCCCCCA CTGGTTCTGG GAAAACTGTA GTGTTTGAGC TAGCGATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GCTAAACATG AAGATTGTTT ACATGGCACC AATAAAAGCC
    CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAGTTTG GACCAGTAGG CTTGAATTGT
    AAAGAACTTA CTGGTGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCAAATATA
    ATTATAACAA CTCCAGAGAA GTGGGACAGT GTGACTAGGA AGTGGAGAGA CAACTCTTTT
    ATCCAACTGG TTCGACTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTTT ATCTAGGGAT
    TTAGAAAGTG CCAGCCCTGT TCCAGTGAGA TTTGTAGCTG TGTCTGCAAC AATTCCCAAT
    GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGTGAGAGAC CTGCTGTATG TCTCAAAATG
    GATGAGAGCC ATAGACCTGT GAAACTTCAG AAAGTAGTCC TGGGTTTTCC CTGTAGTAGT
    AGCCAAACTG AATTTAAGTT TGATTTGGCC CTCAACTATA AAGTGTACAG TGTTATTCGA
    ACATACTCCG ATCAGAAGCC CACACTTGTG TTTTGTTCAA CAAGGAAAGG TGTGCAGCAG
    GCTGCTTCTG TTCTTGTGAA GGATGCTAAG TTTATCATCT CTGTGGAGCA GAAACTACGG
    TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTGA AAGATACCTT GGTATATGGT
    GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGGATTGTTT
    ACCTCTGGAG ATCTACCAGT TCTTTTCACT ACCAGTACTC TAGCTATGGG CATGAACATG
    CCGGCTCACC TGGTTGTTAT AAAGTCTACC ATGCACTACA GTGGAGGTGT GTTTGAAGAG
    TACAGCGAGA CCGACATCCT GCAGATGATT GGGAGAGCTG GCAGACCCCA GTTTGACACT
    ACAGCTACTG CAGTCATTAT GACTCGACTA AGTACAAGAG AGAAGTACGT GCAGATGCTA
    GCTTGTAACG ACACTGTAGA GAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAAATAGTGC TGCATACCAT CACAGACGTG AACATTGCTC TGGACTGGAT CCGATCAACA
    ATGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTTCATC TGGATTGAAC
    AAAGATGGGA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA AGATTTATCA
    TCACTAGACC TGATAAAGAT GGATGAAGAT GTTAATTTTA AACCAACAGA GGCAGGAAGA
    CTAATGGCTT GGTATTATAT TACATTTGAG ACAGTCAAGA AATTTTGTGC AATCAGTGGA
    AAAGAAACTT TATTGGATTT AATTTCAATG ATATCGAGCT GCAATGAGTT TCTAGATGTG
    CAGCTGAGGA TAAGTGAAAA GAGAATACTG AACACCTTAA ACAAAGACCC AAACCGGATA
    ACTATCAGAT TTCCAATGGC AGAAAGAATT AAAACAAGAG AAATGAAAGT AAACTGTCTT
    ATTCAGGCTC AGCTAGGATG CATTCCTATA CAAGATTTTG CTTTGACACA AGATACAGTG
    AAGATTTTCA GAAATGGCTC ACGAATTGCA AGATGGTTGT CAGATTTTGT GGCTGCTCAG
    GAAAAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAA CTAAATGTTT TAAGTGTAAA
    CTCTGGGAAA ACTCTAAACA TGTATCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA
    AATACCATGG TAAATGCAGG CTTAACTTCT TTTAAAAAAA TAGAAGAAGC AAATGCAAGA
    GAACTTGAAC TGATTTTAAA TAGACACCCA CCATTTGGGA CCCAGATAAA AGAAGCTGTG
    GCGCATCTGC CGAAGTATGA GCTTGAGGTG GAGCAGATTG CAAGATACAG TGATATCAAG
    GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAAAC TAAAAGAACA
    GCACCAGACT TCCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTTT
    AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT
    GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGTTC TGATTATGTT
    GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTCG GACCCAGGAA GTTTGTAAAT
    GAAACAGCTA TGGAAAGAAG CTCAGAAACA GATATTTCTC ATTCTGACTA CTCAGGTAGA
    GCCACTGCAA CAGGATCCAG TAAAGGAATG GCTACCTGCA AAAAACCTGG GAACAGAGAA
    TGCCATCATC ACTGTAAAAA TAAACACGCA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAAAAGC CGGAAGTTAA AGAGTCAGCA ATGTCTTCAT ATCTGTCTGA CTTAAAAAGC
    AGAGATGCTG TTTCTTCTCT TCCTCTAGCC AAGCGACTCA AGATACAGAT GAACAAATCT
    CAAAATGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAGGC CTTCCCTTTC CAGTATATCA
    AGGTCAGAAT ATTTAAATAC CCCTGAATTG TCAATATTAG AACAGAGGAA TCAGCATGAA
    ATCTATGGAA AAGTTCAACA GGGACCATCT GAATATCGTG ACAAAGATTT TCCTAGATGG
    GACTATTGTA CAACTTCAAG TGAAAAGACC CCTCAAACGG TGATGTTCAG CAGGCATGCT
    AATATACCTG GAAACCACAG AGTTGCAGAT GAAGACAAAA GCAAGTCTTC AGAAGTTCTG
    GGTGTGAACT TAGAGCTGGG AAATGAAGTC TGGGATGACT TTGATGATGA GAGCTTAATA
    GAAGTTATGA GCCTTTCAGC TGATGCTGAG AAGATGGCTG CATCAGGATT TGGAGACACT
    CGTGATTCAA GTCTCGGCGG AAGTAAGCTA CCCTTCCAAA AGTCAAGCAG CAGATTCCAA
    AGAGACAACT CAAACAGTTT TGCTTCGTCG CCTGGGAAGC CAGATGCTTA TTTGCGAGAT
    TGTTCCAGGT CCAGCTTCGG GCTGTCCTCT GTGGCAGAGA TCCCTCAGCG GGCTGAGAAT
    GCAAGTCTTG CCAATTTACA AGAGAGGAGA CCGCTGACTC TCTCACCAGT GATTGAGAGG
    ATGTGCTTTG CCCACTCTAA GAAAACACCA CAGTCTCCCA AGTTTAAGGA AGTGGATATT
    TTTATTGGAA ACAGTGGCTC TAAAAAGGAA ATTGATCTTA GTAAGTATTA TCCTGATGAT
    GCAGCTGAAG AAATGAAGGC TCTTCTGGGA ATATTTAATG GCATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74855
    Clone ID Related Accession (Same CDS sequence) XM_011249485.1
    Accession Version XM_011249485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGCC CAGTGAAACT TACCCAAACG AAAATAAGCA AAGAGAATGC ATGGACGTGT
    AGCAATAGTA AGCAGAAACG TCAGTATTCT ACAAACAAAT TCAAAGCAAA TGATGCTTTT
    TCTGCTTCTG GAATTGGAAA AGACATATTC AAAGCACCAT CATTTCCTGC TGCATCTCAG
    CCTCATGATA TTCAAGGAAT AACCCCAAAT GGCTTAGGTT CTTTGAAAGC TGTCACGGAA
    ATCCCGGCAA AATTTAGAAA TATATTCAAA GAATTCCCTT ACTTCAACTA TATACAATCC
    AAAGCCTTTG ATGATCTTCT TTACACAGAT AGGAATTTTG TGATTTGTGC CCCCACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAGCTAGCG ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGCTAA ACATGAAGAT TGTTTACATG GCACCAATAA AAGCCCTTTG TAGTCAGCGG
    TTTGATGACT GGAAAGAAAA GTTTGGACCA GTAGGCTTGA ATTGTAAAGA ACTTACTGGT
    GATACAGTAA TGGATGACCT GTTTGAGATT CAGCATGCAA ATATAATTAT AACAACTCCA
    GAGAAGTGGG ACAGTGTGAC TAGGAAGTGG AGAGACAACT CTTTTATCCA ACTGGTTCGA
    CTGTTTCTCA TTGATGAGGT GCATGTTATT AAAGATGAAA ATCGTGGTCC AACTCTTGAA
    GTTGTAGTTA GCAGAATGAA AACTGTACAG TCTTTATCTA GGGATTTAGA AAGTGCCAGC
    CCTGTTCCAG TGAGATTTGT AGCTGTGTCT GCAACAATTC CCAATGCTGA GGATATCGCA
    GAATGGCTTT CAGATGGTGA GAGACCTGCT GTATGTCTCA AAATGGATGA GAGCCATAGA
    CCTGTGAAAC TTCAGAAAGT AGTCCTGGGT TTTCCCTGTA GTAGTAGCCA AACTGAATTT
    AAGTTTGATT TGGCCCTCAA CTATAAAGTG TACAGTGTTA TTCGAACATA CTCCGATCAG
    AAGCCCACAC TTGTGTTTTG TTCAACAAGG AAAGGTGTGC AGCAGGCTGC TTCTGTTCTT
    GTGAAGGATG CTAAGTTTAT CATCTCTGTG GAGCAGAAAC TACGGTTGCA GAAGTCTGCA
    TATTCTATAA GGGATTCAAA GCTGAAAGAT ACCTTGGTAT ATGGTGTTGG TTACCATCAT
    GCTGGAATGG AGCTGTCAGA TAGAAAATTG GTTGAAGGAT TGTTTACCTC TGGAGATCTA
    CCAGTTCTTT TCACTACCAG TACTCTAGCT ATGGGCATGA ACATGCCGGC TCACCTGGTT
    GTTATAAAGT CTACCATGCA CTACAGTGGA GGTGTGTTTG AAGAGTACAG CGAGACCGAC
    ATCCTGCAGA TGATTGGGAG AGCTGGCAGA CCCCAGTTTG ACACTACAGC TACTGCAGTC
    ATTATGACTC GACTAAGTAC AAGAGAGAAG TACGTGCAGA TGCTAGCTTG TAACGACACT
    GTAGAGAGCA GTTTGCACAG ACATCTTATT GAACATTTAA ATGCAGAAAT AGTGCTGCAT
    ACCATCACAG ACGTGAACAT TGCTCTGGAC TGGATCCGAT CAACAATGCT GTATATCAGA
    GCTCTGAAAA ACCCATCTCA CTATGGTTTT TCATCTGGAT TGAACAAAGA TGGGATTGAA
    GCAAAATTAC AAGAATTATG TTTGAAGAAT CTGAAAGATT TATCATCACT AGACCTGATA
    AAGATGGATG AAGATGTTAA TTTTAAACCA ACAGAGGCAG GAAGACTAAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT CAAGAAATTT TGTGCAATCA GTGGAAAAGA AACTTTATTG
    GATTTAATTT CAATGATATC GAGCTGCAAT GAGTTTCTAG ATGTGCAGCT GAGGATAAGT
    GAAAAGAGAA TACTGAACAC CTTAAACAAA GACCCAAACC GGATAACTAT CAGATTTCCA
    ATGGCAGAAA GAATTAAAAC AAGAGAAATG AAAGTAAACT GTCTTATTCA GGCTCAGCTA
    GGATGCATTC CTATACAAGA TTTTGCTTTG ACACAAGATA CAGTGAAGAT TTTCAGAAAT
    GGCTCACGAA TTGCAAGATG GTTGTCAGAT TTTGTGGCTG CTCAGGAAAA GAAGTTTGCT
    GTGCTTTTAA ACAGTGTGAT TTTAACTAAA TGTTTTAAGT GTAAACTCTG GGAAAACTCT
    AAACATGTAT CAAAACAGCT GGATAAAATT GGCATATCAT TGTCAAATAC CATGGTAAAT
    GCAGGCTTAA CTTCTTTTAA AAAAATAGAA GAAGCAAATG CAAGAGAACT TGAACTGATT
    TTAAATAGAC ACCCACCATT TGGGACCCAG ATAAAAGAAG CTGTGGCGCA TCTGCCGAAG
    TATGAGCTTG AGGTGGAGCA GATTGCAAGA TACAGTGATA TCAAGGCAGA AATACTGGTG
    ACCATTATAT TAAGAAACTT TGAGCAGCTG CAAACTAAAA GAACAGCACC AGACTTCCAC
    TATGCCACCT TAATCATAGG TGATGCAGAT AACCAAGTCG TTTTTAAGCA CAAAATTATG
    GATTCTGTCT TGTTAAAAAG TGGAAATTGG GTTAAAAAAA TTGATGTGAA GAGAGCTCTT
    ATATCAGAAG ATCTCAGCAT AAATCTAATT AGTTCTGATT ATGTTGGTCT TGATATTCAC
    CAGAAATTTA CAGTCTTTTA TTTCGGACCC AGGAAGTTTG TAAATGAAAC AGCTATGGAA
    AGAAGCTCAG AAACAGATAT TTCTCATTCT GACTACTCAG GTAGAGCCAC TGCAACAGGA
    TCCAGTAAAG GAATGGCTAC CTGCAAAAAA CCTGGGAACA GAGAATGCCA TCATCACTGT
    AAAAATAAAC ACGCATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA AAAGCCGGAA
    GTTAAAGAGT CAGCAATGTC TTCATATCTG TCTGACTTAA AAAGCAGAGA TGCTGTTTCT
    TCTCTTCCTC TAGCCAAGCG ACTCAAGATA CAGATGAACA AATCTCAAAA TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAGGCCTTCC CTTTCCAGTA TATCAAGGTC AGAATATTTA
    AATACCCCTG AATTGTCAAT ATTAGAACAG AGGAATCAGC ATGAAATCTA TGGAAAAGTT
    CAACAGGGAC CATCTGAATA TCGTGACAAA GATTTTCCTA GATGGGACTA TTGTACAACT
    TCAAGTGAAA AGACCCCTCA AACGGTGATG TTCAGCAGGC ATGCTAATAT ACCTGGAAAC
    CACAGAGTTG CAGATGAAGA CAAAAGCAAG TCTTCAGAAG TTCTGGGTGT GAACTTAGAG
    CTGGGAAATG AAGTCTGGGA TGACTTTGAT GATGAGAGCT TAATAGAAGT TATGAGCCTT
    TCAGCTGATG CTGAGAAGAT GGCTGCATCA GGATTTGGAG ACACTCGTGA TTCAAGTCTC
    GGCGGAAGTA AGCTACCCTT CCAAAAGTCA AGCAGCAGAT TCCAAAGAGA CAACTCAAAC
    AGTTTTGCTT CGTCGCCTGG GAAGCCAGAT GCTTATTTGC GAGATTGTTC CAGGTCCAGC
    TTCGGGCTGT CCTCTGTGGC AGAGATCCCT CAGCGGGCTG AGAATGCAAG TCTTGCCAAT
    TTACAAGAGA GGAGACCGCT GACTCTCTCA CCAGTGATTG AGAGGATGTG CTTTGCCCAC
    TCTAAGAAAA CACCACAGTC TCCCAAGTTT AAGGAAGTGG ATATTTTTAT TGGAAACAGT
    GGCTCTAAAA AGGAAATTGA TCTTAGTAAG TATTATCCTG ATGATGCAGC TGAAGAAATG
    AAGGCTCTTC TGGGAATATT TAATGGCATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247787.1
    CDS211..4566
    Translation

    Target ORF information:

    RefSeq Version XM_011249485.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA ACTTTCCAGT TAAGGAAAAA TCACTGGATT GGTTTCTCCC TCCTGCTCCA
    CTGATTTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAG TTATAAGCTA
    CTAGGGAAAG GAAAGATGCC AAGAATGTTA ACATCAAACT TGAAGATCAT TAATGAAGAC
    ACAAATTGTA TTTCACCAAC ACAAAAAATC CATTTTTCCT ATAATGTACA TGAACAGGAT
    TACCTAAATT TAGGAGGATC AAATAACAAT GACATGTCCC ATGTAGCTGG GAAGCTGATG
    TATGGTTCTT CTCAGAAATA TAAAAATCAC ATGGGTGCTA AGAGTCCATC TGCAAGGAGT
    AGTCCTGGTG ATACAAAATT ACATGATGTG GCAGAAGACA GACAGGGCAC ATCAGCATTC
    AAAAAAAGCC CAGTGAAACT TACCCAAACG AAAATAAGCA AAGAGAATGC ATGGACGTGT
    AGCAATAGTA AGCAGAAACG TCAGTATTCT ACAAACAAAT TCAAAGCAAA TGATGCTTTT
    TCTGCTTCTG GAATTGGAAA AGACATATTC AAAGCACCAT CATTTCCTGC TGCATCTCAG
    CCTCATGATA TTCAAGGAAT AACCCCAAAT GGCTTAGGTT CTTTGAAAGC TGTCACGGAA
    ATCCCGGCAA AATTTAGAAA TATATTCAAA GAATTCCCTT ACTTCAACTA TATACAATCC
    AAAGCCTTTG ATGATCTTCT TTACACAGAT AGGAATTTTG TGATTTGTGC CCCCACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAGCTAGCG ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGCTAA ACATGAAGAT TGTTTACATG GCACCAATAA AAGCCCTTTG TAGTCAGCGG
    TTTGATGACT GGAAAGAAAA GTTTGGACCA GTAGGCTTGA ATTGTAAAGA ACTTACTGGT
    GATACAGTAA TGGATGACCT GTTTGAGATT CAGCATGCAA ATATAATTAT AACAACTCCA
    GAGAAGTGGG ACAGTGTGAC TAGGAAGTGG AGAGACAACT CTTTTATCCA ACTGGTTCGA
    CTGTTTCTCA TTGATGAGGT GCATGTTATT AAAGATGAAA ATCGTGGTCC AACTCTTGAA
    GTTGTAGTTA GCAGAATGAA AACTGTACAG TCTTTATCTA GGGATTTAGA AAGTGCCAGC
    CCTGTTCCAG TGAGATTTGT AGCTGTGTCT GCAACAATTC CCAATGCTGA GGATATCGCA
    GAATGGCTTT CAGATGGTGA GAGACCTGCT GTATGTCTCA AAATGGATGA GAGCCATAGA
    CCTGTGAAAC TTCAGAAAGT AGTCCTGGGT TTTCCCTGTA GTAGTAGCCA AACTGAATTT
    AAGTTTGATT TGGCCCTCAA CTATAAAGTG TACAGTGTTA TTCGAACATA CTCCGATCAG
    AAGCCCACAC TTGTGTTTTG TTCAACAAGG AAAGGTGTGC AGCAGGCTGC TTCTGTTCTT
    GTGAAGGATG CTAAGTTTAT CATCTCTGTG GAGCAGAAAC TACGGTTGCA GAAGTCTGCA
    TATTCTATAA GGGATTCAAA GCTGAAAGAT ACCTTGGTAT ATGGTGTTGG TTACCATCAT
    GCTGGAATGG AGCTGTCAGA TAGAAAATTG GTTGAAGGAT TGTTTACCTC TGGAGATCTA
    CCAGTTCTTT TCACTACCAG TACTCTAGCT ATGGGCATGA ACATGCCGGC TCACCTGGTT
    GTTATAAAGT CTACCATGCA CTACAGTGGA GGTGTGTTTG AAGAGTACAG CGAGACCGAC
    ATCCTGCAGA TGATTGGGAG AGCTGGCAGA CCCCAGTTTG ACACTACAGC TACTGCAGTC
    ATTATGACTC GACTAAGTAC AAGAGAGAAG TACGTGCAGA TGCTAGCTTG TAACGACACT
    GTAGAGAGCA GTTTGCACAG ACATCTTATT GAACATTTAA ATGCAGAAAT AGTGCTGCAT
    ACCATCACAG ACGTGAACAT TGCTCTGGAC TGGATCCGAT CAACAATGCT GTATATCAGA
    GCTCTGAAAA ACCCATCTCA CTATGGTTTT TCATCTGGAT TGAACAAAGA TGGGATTGAA
    GCAAAATTAC AAGAATTATG TTTGAAGAAT CTGAAAGATT TATCATCACT AGACCTGATA
    AAGATGGATG AAGATGTTAA TTTTAAACCA ACAGAGGCAG GAAGACTAAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT CAAGAAATTT TGTGCAATCA GTGGAAAAGA AACTTTATTG
    GATTTAATTT CAATGATATC GAGCTGCAAT GAGTTTCTAG ATGTGCAGCT GAGGATAAGT
    GAAAAGAGAA TACTGAACAC CTTAAACAAA GACCCAAACC GGATAACTAT CAGATTTCCA
    ATGGCAGAAA GAATTAAAAC AAGAGAAATG AAAGTAAACT GTCTTATTCA GGCTCAGCTA
    GGATGCATTC CTATACAAGA TTTTGCTTTG ACACAAGATA CAGTGAAGAT TTTCAGAAAT
    GGCTCACGAA TTGCAAGATG GTTGTCAGAT TTTGTGGCTG CTCAGGAAAA GAAGTTTGCT
    GTGCTTTTAA ACAGTGTGAT TTTAACTAAA TGTTTTAAGT GTAAACTCTG GGAAAACTCT
    AAACATGTAT CAAAACAGCT GGATAAAATT GGCATATCAT TGTCAAATAC CATGGTAAAT
    GCAGGCTTAA CTTCTTTTAA AAAAATAGAA GAAGCAAATG CAAGAGAACT TGAACTGATT
    TTAAATAGAC ACCCACCATT TGGGACCCAG ATAAAAGAAG CTGTGGCGCA TCTGCCGAAG
    TATGAGCTTG AGGTGGAGCA GATTGCAAGA TACAGTGATA TCAAGGCAGA AATACTGGTG
    ACCATTATAT TAAGAAACTT TGAGCAGCTG CAAACTAAAA GAACAGCACC AGACTTCCAC
    TATGCCACCT TAATCATAGG TGATGCAGAT AACCAAGTCG TTTTTAAGCA CAAAATTATG
    GATTCTGTCT TGTTAAAAAG TGGAAATTGG GTTAAAAAAA TTGATGTGAA GAGAGCTCTT
    ATATCAGAAG ATCTCAGCAT AAATCTAATT AGTTCTGATT ATGTTGGTCT TGATATTCAC
    CAGAAATTTA CAGTCTTTTA TTTCGGACCC AGGAAGTTTG TAAATGAAAC AGCTATGGAA
    AGAAGCTCAG AAACAGATAT TTCTCATTCT GACTACTCAG GTAGAGCCAC TGCAACAGGA
    TCCAGTAAAG GAATGGCTAC CTGCAAAAAA CCTGGGAACA GAGAATGCCA TCATCACTGT
    AAAAATAAAC ACGCATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA AAAGCCGGAA
    GTTAAAGAGT CAGCAATGTC TTCATATCTG TCTGACTTAA AAAGCAGAGA TGCTGTTTCT
    TCTCTTCCTC TAGCCAAGCG ACTCAAGATA CAGATGAACA AATCTCAAAA TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAGGCCTTCC CTTTCCAGTA TATCAAGGTC AGAATATTTA
    AATACCCCTG AATTGTCAAT ATTAGAACAG AGGAATCAGC ATGAAATCTA TGGAAAAGTT
    CAACAGGGAC CATCTGAATA TCGTGACAAA GATTTTCCTA GATGGGACTA TTGTACAACT
    TCAAGTGAAA AGACCCCTCA AACGGTGATG TTCAGCAGGC ATGCTAATAT ACCTGGAAAC
    CACAGAGTTG CAGATGAAGA CAAAAGCAAG TCTTCAGAAG TTCTGGGTGT GAACTTAGAG
    CTGGGAAATG AAGTCTGGGA TGACTTTGAT GATGAGAGCT TAATAGAAGT TATGAGCCTT
    TCAGCTGATG CTGAGAAGAT GGCTGCATCA GGATTTGGAG ACACTCGTGA TTCAAGTCTC
    GGCGGAAGTA AGCTACCCTT CCAAAAGTCA AGCAGCAGAT TCCAAAGAGA CAACTCAAAC
    AGTTTTGCTT CGTCGCCTGG GAAGCCAGAT GCTTATTTGC GAGATTGTTC CAGGTCCAGC
    TTCGGGCTGT CCTCTGTGGC AGAGATCCCT CAGCGGGCTG AGAATGCAAG TCTTGCCAAT
    TTACAAGAGA GGAGACCGCT GACTCTCTCA CCAGTGATTG AGAGGATGTG CTTTGCCCAC
    TCTAAGAAAA CACCACAGTC TCCCAAGTTT AAGGAAGTGG ATATTTTTAT TGGAAACAGT
    GGCTCTAAAA AGGAAATTGA TCTTAGTAAG TATTATCCTG ATGATGCAGC TGAAGAAATG
    AAGGCTCTTC TGGGAATATT TAATGGCATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74856
    Clone ID Related Accession (Same CDS sequence) XM_030254593.1 , XM_011249486.1
    Accession Version XM_011249486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAACTGA AAAGGAAAGG AAAGATGCCA AGAATGTTAA CATCAAACTT GAAGATCATT 
    AATGAAGACA CAAATTGTAT TTCACCAACA CAAAAAATCC ATTTTTCCTA TAATGTACAT
    GAACAGGATT ACCTAAATTT AGGAGGATCA AATAACAATG ACATGTCCCA TGTAGCTGGG
    AAGCTGATGT ATGGTTCTTC TCAGAAATAT AAAAATCACA TGGGTGCTAA GAGTCCATCT
    GCAAGGAGTA GTCCTGGTGA TACAAAATTA CATGATGTGG CAGAAGACAG ACAGGGCACA
    TCAGCATTCA AAAAAAGACT GTCTAGAACA TGTGACAGTG AACATGACTG TGACTATGCT
    GATGGAAGTT TAAACTTGAG TTCTCATATC AGCCCAGTGA AACTTACCCA AACGAAAATA
    AGCAAAGAGA ATGCATGGAC GTGTAGCAAT AGTAAGCAGA AACGTCAGTA TTCTACAAAC
    AAATTCAAAG CAAATGATGC TTTTTCTGCT TCTGGAATTG GAAAAGACAT ATTCAAAGCA
    CCATCATTTC CTGCTGCATC TCAGCCTCAT GATATTCAAG GAATAACCCC AAATGGCTTA
    GGTTCTTTGA AAGCTGTCAC GGAAATCCCG GCAAAATTTA GAAATATATT CAAAGAATTC
    CCTTACTTCA ACTATATACA ATCCAAAGCC TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCCCCCAC TGGTTCTGGG AAAACTGTAG TGTTTGAGCT AGCGATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG CTAAACATGA AGATTGTTTA CATGGCACCA
    ATAAAAGCCC TTTGTAGTCA GCGGTTTGAT GACTGGAAAG AAAAGTTTGG ACCAGTAGGC
    TTGAATTGTA AAGAACTTAC TGGTGATACA GTAATGGATG ACCTGTTTGA GATTCAGCAT
    GCAAATATAA TTATAACAAC TCCAGAGAAG TGGGACAGTG TGACTAGGAA GTGGAGAGAC
    AACTCTTTTA TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTGCATGT TATTAAAGAT
    GAAAATCGTG GTCCAACTCT TGAAGTTGTA GTTAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTAGGGATT TAGAAAGTGC CAGCCCTGTT CCAGTGAGAT TTGTAGCTGT GTCTGCAACA
    ATTCCCAATG CTGAGGATAT CGCAGAATGG CTTTCAGATG GTGAGAGACC TGCTGTATGT
    CTCAAAATGG ATGAGAGCCA TAGACCTGTG AAACTTCAGA AAGTAGTCCT GGGTTTTCCC
    TGTAGTAGTA GCCAAACTGA ATTTAAGTTT GATTTGGCCC TCAACTATAA AGTGTACAGT
    GTTATTCGAA CATACTCCGA TCAGAAGCCC ACACTTGTGT TTTGTTCAAC AAGGAAAGGT
    GTGCAGCAGG CTGCTTCTGT TCTTGTGAAG GATGCTAAGT TTATCATCTC TGTGGAGCAG
    AAACTACGGT TGCAGAAGTC TGCATATTCT ATAAGGGATT CAAAGCTGAA AGATACCTTG
    GTATATGGTG TTGGTTACCA TCATGCTGGA ATGGAGCTGT CAGATAGAAA ATTGGTTGAA
    GGATTGTTTA CCTCTGGAGA TCTACCAGTT CTTTTCACTA CCAGTACTCT AGCTATGGGC
    ATGAACATGC CGGCTCACCT GGTTGTTATA AAGTCTACCA TGCACTACAG TGGAGGTGTG
    TTTGAAGAGT ACAGCGAGAC CGACATCCTG CAGATGATTG GGAGAGCTGG CAGACCCCAG
    TTTGACACTA CAGCTACTGC AGTCATTATG ACTCGACTAA GTACAAGAGA GAAGTACGTG
    CAGATGCTAG CTTGTAACGA CACTGTAGAG AGCAGTTTGC ACAGACATCT TATTGAACAT
    TTAAATGCAG AAATAGTGCT GCATACCATC ACAGACGTGA ACATTGCTCT GGACTGGATC
    CGATCAACAA TGCTGTATAT CAGAGCTCTG AAAAACCCAT CTCACTATGG TTTTTCATCT
    GGATTGAACA AAGATGGGAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGAAA
    GATTTATCAT CACTAGACCT GATAAAGATG GATGAAGATG TTAATTTTAA ACCAACAGAG
    GCAGGAAGAC TAATGGCTTG GTATTATATT ACATTTGAGA CAGTCAAGAA ATTTTGTGCA
    ATCAGTGGAA AAGAAACTTT ATTGGATTTA ATTTCAATGA TATCGAGCTG CAATGAGTTT
    CTAGATGTGC AGCTGAGGAT AAGTGAAAAG AGAATACTGA ACACCTTAAA CAAAGACCCA
    AACCGGATAA CTATCAGATT TCCAATGGCA GAAAGAATTA AAACAAGAGA AATGAAAGTA
    AACTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCTATAC AAGATTTTGC TTTGACACAA
    GATACAGTGA AGATTTTCAG AAATGGCTCA CGAATTGCAA GATGGTTGTC AGATTTTGTG
    GCTGCTCAGG AAAAGAAGTT TGCTGTGCTT TTAAACAGTG TGATTTTAAC TAAATGTTTT
    AAGTGTAAAC TCTGGGAAAA CTCTAAACAT GTATCAAAAC AGCTGGATAA AATTGGCATA
    TCATTGTCAA ATACCATGGT AAATGCAGGC TTAACTTCTT TTAAAAAAAT AGAAGAAGCA
    AATGCAAGAG AACTTGAACT GATTTTAAAT AGACACCCAC CATTTGGGAC CCAGATAAAA
    GAAGCTGTGG CGCATCTGCC GAAGTATGAG CTTGAGGTGG AGCAGATTGC AAGATACAGT
    GATATCAAGG CAGAAATACT GGTGACCATT ATATTAAGAA ACTTTGAGCA GCTGCAAACT
    AAAAGAACAG CACCAGACTT CCACTATGCC ACCTTAATCA TAGGTGATGC AGATAACCAA
    GTCGTTTTTA AGCACAAAAT TATGGATTCT GTCTTGTTAA AAAGTGGAAA TTGGGTTAAA
    AAAATTGATG TGAAGAGAGC TCTTATATCA GAAGATCTCA GCATAAATCT AATTAGTTCT
    GATTATGTTG GTCTTGATAT TCACCAGAAA TTTACAGTCT TTTATTTCGG ACCCAGGAAG
    TTTGTAAATG AAACAGCTAT GGAAAGAAGC TCAGAAACAG ATATTTCTCA TTCTGACTAC
    TCAGGTAGAG CCACTGCAAC AGGATCCAGT AAAGGAATGG CTACCTGCAA AAAACCTGGG
    AACAGAGAAT GCCATCATCA CTGTAAAAAT AAACACGCAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAAAAGCC GGAAGTTAAA GAGTCAGCAA TGTCTTCATA TCTGTCTGAC
    TTAAAAAGCA GAGATGCTGT TTCTTCTCTT CCTCTAGCCA AGCGACTCAA GATACAGATG
    AACAAATCTC AAAATGTGGA CCTTAAAGAG TTTGGTTTTA CTCCAAGGCC TTCCCTTTCC
    AGTATATCAA GGTCAGAATA TTTAAATACC CCTGAATTGT CAATATTAGA ACAGAGGAAT
    CAGCATGAAA TCTATGGAAA AGTTCAACAG GGACCATCTG AATATCGTGA CAAAGATTTT
    CCTAGATGGG ACTATTGTAC AACTTCAAGT GAAAAGACCC CTCAAACGGT GATGTTCAGC
    AGGCATGCTA ATATACCTGG AAACCACAGA GTTGCAGATG AAGACAAAAG CAAGTCTTCA
    GAAGTTCTGG GTGTGAACTT AGAGCTGGGA AATGAAGTCT GGGATGACTT TGATGATGAG
    AGCTTAATAG AAGTTATGAG CCTTTCAGCT GATGCTGAGA AGATGGCTGC ATCAGGATTT
    GGAGACACTC GTGATTCAAG TCTCGGCGGA AGTAAGCTAC CCTTCCAAAA GTCAAGCAGC
    AGATTCCAAA GAGACAACTC AAACAGTTTT GCTTCGTCGC CTGGGAAGCC AGATGCTTAT
    TTGCGAGATT GTTCCAGGTC CAGCTTCGGG CTGTCCTCTG TGGCAGAGAT CCCTCAGCGG
    GCTGAGAATG CAAGTCTTGC CAATTTACAA GAGAGGAGAC CGCTGACTCT CTCACCAGTG
    ATTGAGAGGA TGTGCTTTGC CCACTCTAAG AAAACACCAC AGTCTCCCAA GTTTAAGGAA
    GTGGATATTT TTATTGGAAA CAGTGGCTCT AAAAAGGAAA TTGATCTTAG TAAGTATTAT
    CCTGATGATG CAGCTGAAGA AATGAAGGCT CTTCTGGGAA TATTTAATGG CATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247788.1
    CDS86..4345
    Translation

    Target ORF information:

    RefSeq Version XM_011249486.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAACTGA AAAGGAAAGG AAAGATGCCA AGAATGTTAA CATCAAACTT GAAGATCATT 
    AATGAAGACA CAAATTGTAT TTCACCAACA CAAAAAATCC ATTTTTCCTA TAATGTACAT
    GAACAGGATT ACCTAAATTT AGGAGGATCA AATAACAATG ACATGTCCCA TGTAGCTGGG
    AAGCTGATGT ATGGTTCTTC TCAGAAATAT AAAAATCACA TGGGTGCTAA GAGTCCATCT
    GCAAGGAGTA GTCCTGGTGA TACAAAATTA CATGATGTGG CAGAAGACAG ACAGGGCACA
    TCAGCATTCA AAAAAAGACT GTCTAGAACA TGTGACAGTG AACATGACTG TGACTATGCT
    GATGGAAGTT TAAACTTGAG TTCTCATATC AGCCCAGTGA AACTTACCCA AACGAAAATA
    AGCAAAGAGA ATGCATGGAC GTGTAGCAAT AGTAAGCAGA AACGTCAGTA TTCTACAAAC
    AAATTCAAAG CAAATGATGC TTTTTCTGCT TCTGGAATTG GAAAAGACAT ATTCAAAGCA
    CCATCATTTC CTGCTGCATC TCAGCCTCAT GATATTCAAG GAATAACCCC AAATGGCTTA
    GGTTCTTTGA AAGCTGTCAC GGAAATCCCG GCAAAATTTA GAAATATATT CAAAGAATTC
    CCTTACTTCA ACTATATACA ATCCAAAGCC TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCCCCCAC TGGTTCTGGG AAAACTGTAG TGTTTGAGCT AGCGATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG CTAAACATGA AGATTGTTTA CATGGCACCA
    ATAAAAGCCC TTTGTAGTCA GCGGTTTGAT GACTGGAAAG AAAAGTTTGG ACCAGTAGGC
    TTGAATTGTA AAGAACTTAC TGGTGATACA GTAATGGATG ACCTGTTTGA GATTCAGCAT
    GCAAATATAA TTATAACAAC TCCAGAGAAG TGGGACAGTG TGACTAGGAA GTGGAGAGAC
    AACTCTTTTA TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTGCATGT TATTAAAGAT
    GAAAATCGTG GTCCAACTCT TGAAGTTGTA GTTAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTAGGGATT TAGAAAGTGC CAGCCCTGTT CCAGTGAGAT TTGTAGCTGT GTCTGCAACA
    ATTCCCAATG CTGAGGATAT CGCAGAATGG CTTTCAGATG GTGAGAGACC TGCTGTATGT
    CTCAAAATGG ATGAGAGCCA TAGACCTGTG AAACTTCAGA AAGTAGTCCT GGGTTTTCCC
    TGTAGTAGTA GCCAAACTGA ATTTAAGTTT GATTTGGCCC TCAACTATAA AGTGTACAGT
    GTTATTCGAA CATACTCCGA TCAGAAGCCC ACACTTGTGT TTTGTTCAAC AAGGAAAGGT
    GTGCAGCAGG CTGCTTCTGT TCTTGTGAAG GATGCTAAGT TTATCATCTC TGTGGAGCAG
    AAACTACGGT TGCAGAAGTC TGCATATTCT ATAAGGGATT CAAAGCTGAA AGATACCTTG
    GTATATGGTG TTGGTTACCA TCATGCTGGA ATGGAGCTGT CAGATAGAAA ATTGGTTGAA
    GGATTGTTTA CCTCTGGAGA TCTACCAGTT CTTTTCACTA CCAGTACTCT AGCTATGGGC
    ATGAACATGC CGGCTCACCT GGTTGTTATA AAGTCTACCA TGCACTACAG TGGAGGTGTG
    TTTGAAGAGT ACAGCGAGAC CGACATCCTG CAGATGATTG GGAGAGCTGG CAGACCCCAG
    TTTGACACTA CAGCTACTGC AGTCATTATG ACTCGACTAA GTACAAGAGA GAAGTACGTG
    CAGATGCTAG CTTGTAACGA CACTGTAGAG AGCAGTTTGC ACAGACATCT TATTGAACAT
    TTAAATGCAG AAATAGTGCT GCATACCATC ACAGACGTGA ACATTGCTCT GGACTGGATC
    CGATCAACAA TGCTGTATAT CAGAGCTCTG AAAAACCCAT CTCACTATGG TTTTTCATCT
    GGATTGAACA AAGATGGGAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGAAA
    GATTTATCAT CACTAGACCT GATAAAGATG GATGAAGATG TTAATTTTAA ACCAACAGAG
    GCAGGAAGAC TAATGGCTTG GTATTATATT ACATTTGAGA CAGTCAAGAA ATTTTGTGCA
    ATCAGTGGAA AAGAAACTTT ATTGGATTTA ATTTCAATGA TATCGAGCTG CAATGAGTTT
    CTAGATGTGC AGCTGAGGAT AAGTGAAAAG AGAATACTGA ACACCTTAAA CAAAGACCCA
    AACCGGATAA CTATCAGATT TCCAATGGCA GAAAGAATTA AAACAAGAGA AATGAAAGTA
    AACTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCTATAC AAGATTTTGC TTTGACACAA
    GATACAGTGA AGATTTTCAG AAATGGCTCA CGAATTGCAA GATGGTTGTC AGATTTTGTG
    GCTGCTCAGG AAAAGAAGTT TGCTGTGCTT TTAAACAGTG TGATTTTAAC TAAATGTTTT
    AAGTGTAAAC TCTGGGAAAA CTCTAAACAT GTATCAAAAC AGCTGGATAA AATTGGCATA
    TCATTGTCAA ATACCATGGT AAATGCAGGC TTAACTTCTT TTAAAAAAAT AGAAGAAGCA
    AATGCAAGAG AACTTGAACT GATTTTAAAT AGACACCCAC CATTTGGGAC CCAGATAAAA
    GAAGCTGTGG CGCATCTGCC GAAGTATGAG CTTGAGGTGG AGCAGATTGC AAGATACAGT
    GATATCAAGG CAGAAATACT GGTGACCATT ATATTAAGAA ACTTTGAGCA GCTGCAAACT
    AAAAGAACAG CACCAGACTT CCACTATGCC ACCTTAATCA TAGGTGATGC AGATAACCAA
    GTCGTTTTTA AGCACAAAAT TATGGATTCT GTCTTGTTAA AAAGTGGAAA TTGGGTTAAA
    AAAATTGATG TGAAGAGAGC TCTTATATCA GAAGATCTCA GCATAAATCT AATTAGTTCT
    GATTATGTTG GTCTTGATAT TCACCAGAAA TTTACAGTCT TTTATTTCGG ACCCAGGAAG
    TTTGTAAATG AAACAGCTAT GGAAAGAAGC TCAGAAACAG ATATTTCTCA TTCTGACTAC
    TCAGGTAGAG CCACTGCAAC AGGATCCAGT AAAGGAATGG CTACCTGCAA AAAACCTGGG
    AACAGAGAAT GCCATCATCA CTGTAAAAAT AAACACGCAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAAAAGCC GGAAGTTAAA GAGTCAGCAA TGTCTTCATA TCTGTCTGAC
    TTAAAAAGCA GAGATGCTGT TTCTTCTCTT CCTCTAGCCA AGCGACTCAA GATACAGATG
    AACAAATCTC AAAATGTGGA CCTTAAAGAG TTTGGTTTTA CTCCAAGGCC TTCCCTTTCC
    AGTATATCAA GGTCAGAATA TTTAAATACC CCTGAATTGT CAATATTAGA ACAGAGGAAT
    CAGCATGAAA TCTATGGAAA AGTTCAACAG GGACCATCTG AATATCGTGA CAAAGATTTT
    CCTAGATGGG ACTATTGTAC AACTTCAAGT GAAAAGACCC CTCAAACGGT GATGTTCAGC
    AGGCATGCTA ATATACCTGG AAACCACAGA GTTGCAGATG AAGACAAAAG CAAGTCTTCA
    GAAGTTCTGG GTGTGAACTT AGAGCTGGGA AATGAAGTCT GGGATGACTT TGATGATGAG
    AGCTTAATAG AAGTTATGAG CCTTTCAGCT GATGCTGAGA AGATGGCTGC ATCAGGATTT
    GGAGACACTC GTGATTCAAG TCTCGGCGGA AGTAAGCTAC CCTTCCAAAA GTCAAGCAGC
    AGATTCCAAA GAGACAACTC AAACAGTTTT GCTTCGTCGC CTGGGAAGCC AGATGCTTAT
    TTGCGAGATT GTTCCAGGTC CAGCTTCGGG CTGTCCTCTG TGGCAGAGAT CCCTCAGCGG
    GCTGAGAATG CAAGTCTTGC CAATTTACAA GAGAGGAGAC CGCTGACTCT CTCACCAGTG
    ATTGAGAGGA TGTGCTTTGC CCACTCTAAG AAAACACCAC AGTCTCCCAA GTTTAAGGAA
    GTGGATATTT TTATTGGAAA CAGTGGCTCT AAAAAGGAAA TTGATCTTAG TAAGTATTAT
    CCTGATGATG CAGCTGAAGA AATGAAGGCT CTTCTGGGAA TATTTAATGG CATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74857
    Clone ID Related Accession (Same CDS sequence) XM_011249487.1
    Accession Version XM_011249487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable ATP-dependent DNA helicase HFM1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA AACTGTCTAG AACATGTGAC AGTGAACATG ACTGTGACTA TGCTGATGGA
    AGTTTAAACT TGAGTTCTCA TATCAGCCCA GTGAAACTTA CCCAAACGAA AATAAGCAAA
    GAGAATGCAT GGACGTGTAG CAATAGTAAG CAGAAACGTC AGTATTCTAC AAACAAATTC
    AAAGCAAATG ATGCTTTTTC TGCTTCTGGA ATTGGAAAAG ACATATTCAA AGCACCATCA
    TTTCCTGCTG CATCTCAGCC TCATGATATT CAAGGAATAA CCCCAAATGG CTTAGGTTCT
    TTGAAAGCTG TCACGGAAAT CCCGGCAAAA TTTAGAAATA TATTCAAAGA ATTCCCTTAC
    TTCAACTATA TACAATCCAA AGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCCC CCACTGGTTC TGGGAAAACT GTAGTGTTTG AGCTAGCGAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGCTAAAC ATGAAGATTG TTTACATGGC ACCAATAAAA
    GCCCTTTGTA GTCAGCGGTT TGATGACTGG AAAGAAAAGT TTGGACCAGT AGGCTTGAAT
    TGTAAAGAAC TTACTGGTGA TACAGTAATG GATGACCTGT TTGAGATTCA GCATGCAAAT
    ATAATTATAA CAACTCCAGA GAAGTGGGAC AGTGTGACTA GGAAGTGGAG AGACAACTCT
    TTTATCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTGC ATGTTATTAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TTTATCTAGG
    GATTTAGAAA GTGCCAGCCC TGTTCCAGTG AGATTTGTAG CTGTGTCTGC AACAATTCCC
    AATGCTGAGG ATATCGCAGA ATGGCTTTCA GATGGTGAGA GACCTGCTGT ATGTCTCAAA
    ATGGATGAGA GCCATAGACC TGTGAAACTT CAGAAAGTAG TCCTGGGTTT TCCCTGTAGT
    AGTAGCCAAA CTGAATTTAA GTTTGATTTG GCCCTCAACT ATAAAGTGTA CAGTGTTATT
    CGAACATACT CCGATCAGAA GCCCACACTT GTGTTTTGTT CAACAAGGAA AGGTGTGCAG
    CAGGCTGCTT CTGTTCTTGT GAAGGATGCT AAGTTTATCA TCTCTGTGGA GCAGAAACTA
    CGGTTGCAGA AGTCTGCATA TTCTATAAGG GATTCAAAGC TGAAAGATAC CTTGGTATAT
    GGTGTTGGTT ACCATCATGC TGGAATGGAG CTGTCAGATA GAAAATTGGT TGAAGGATTG
    TTTACCTCTG GAGATCTACC AGTTCTTTTC ACTACCAGTA CTCTAGCTAT GGGCATGAAC
    ATGCCGGCTC ACCTGGTTGT TATAAAGTCT ACCATGCACT ACAGTGGAGG TGTGTTTGAA
    GAGTACAGCG AGACCGACAT CCTGCAGATG ATTGGGAGAG CTGGCAGACC CCAGTTTGAC
    ACTACAGCTA CTGCAGTCAT TATGACTCGA CTAAGTACAA GAGAGAAGTA CGTGCAGATG
    CTAGCTTGTA ACGACACTGT AGAGAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAAATAG TGCTGCATAC CATCACAGAC GTGAACATTG CTCTGGACTG GATCCGATCA
    ACAATGCTGT ATATCAGAGC TCTGAAAAAC CCATCTCACT ATGGTTTTTC ATCTGGATTG
    AACAAAGATG GGATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAAAGATTTA
    TCATCACTAG ACCTGATAAA GATGGATGAA GATGTTAATT TTAAACCAAC AGAGGCAGGA
    AGACTAATGG CTTGGTATTA TATTACATTT GAGACAGTCA AGAAATTTTG TGCAATCAGT
    GGAAAAGAAA CTTTATTGGA TTTAATTTCA ATGATATCGA GCTGCAATGA GTTTCTAGAT
    GTGCAGCTGA GGATAAGTGA AAAGAGAATA CTGAACACCT TAAACAAAGA CCCAAACCGG
    ATAACTATCA GATTTCCAAT GGCAGAAAGA ATTAAAACAA GAGAAATGAA AGTAAACTGT
    CTTATTCAGG CTCAGCTAGG ATGCATTCCT ATACAAGATT TTGCTTTGAC ACAAGATACA
    GTGAAGATTT TCAGAAATGG CTCACGAATT GCAAGATGGT TGTCAGATTT TGTGGCTGCT
    CAGGAAAAGA AGTTTGCTGT GCTTTTAAAC AGTGTGATTT TAACTAAATG TTTTAAGTGT
    AAACTCTGGG AAAACTCTAA ACATGTATCA AAACAGCTGG ATAAAATTGG CATATCATTG
    TCAAATACCA TGGTAAATGC AGGCTTAACT TCTTTTAAAA AAATAGAAGA AGCAAATGCA
    AGAGAACTTG AACTGATTTT AAATAGACAC CCACCATTTG GGACCCAGAT AAAAGAAGCT
    GTGGCGCATC TGCCGAAGTA TGAGCTTGAG GTGGAGCAGA TTGCAAGATA CAGTGATATC
    AAGGCAGAAA TACTGGTGAC CATTATATTA AGAAACTTTG AGCAGCTGCA AACTAAAAGA
    ACAGCACCAG ACTTCCACTA TGCCACCTTA ATCATAGGTG ATGCAGATAA CCAAGTCGTT
    TTTAAGCACA AAATTATGGA TTCTGTCTTG TTAAAAAGTG GAAATTGGGT TAAAAAAATT
    GATGTGAAGA GAGCTCTTAT ATCAGAAGAT CTCAGCATAA ATCTAATTAG TTCTGATTAT
    GTTGGTCTTG ATATTCACCA GAAATTTACA GTCTTTTATT TCGGACCCAG GAAGTTTGTA
    AATGAAACAG CTATGGAAAG AAGCTCAGAA ACAGATATTT CTCATTCTGA CTACTCAGGT
    AGAGCCACTG CAACAGGATC CAGTAAAGGA ATGGCTACCT GCAAAAAACC TGGGAACAGA
    GAATGCCATC ATCACTGTAA AAATAAACAC GCATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAAA AGCCGGAAGT TAAAGAGTCA GCAATGTCTT CATATCTGTC TGACTTAAAA
    AGCAGAGATG CTGTTTCTTC TCTTCCTCTA GCCAAGCGAC TCAAGATACA GATGAACAAA
    TCTCAAAATG TGGACCTTAA AGAGTTTGGT TTTACTCCAA GGCCTTCCCT TTCCAGTATA
    TCAAGGTCAG AATATTTAAA TACCCCTGAA TTGTCAATAT TAGAACAGAG GAATCAGCAT
    GAAATCTATG GAAAAGTTCA ACAGGGACCA TCTGAATATC GTGACAAAGA TTTTCCTAGA
    TGGGACTATT GTACAACTTC AAGTGAAAAG ACCCCTCAAA CGGTGATGTT CAGCAGGCAT
    GCTAATATAC CTGGAAACCA CAGAGTTGCA GATGAAGACA AAAGCAAGTC TTCAGAAGTT
    CTGGGTGTGA ACTTAGAGCT GGGAAATGAA GTCTGGGATG ACTTTGATGA TGAGAGCTTA
    ATAGAAGTTA TGAGCCTTTC AGCTGATGCT GAGAAGATGG CTGCATCAGG ATTTGGAGAC
    ACTCGTGATT CAAGTCTCGG CGGAAGTAAG CTACCCTTCC AAAAGTCAAG CAGCAGATTC
    CAAAGAGACA ACTCAAACAG TTTTGCTTCG TCGCCTGGGA AGCCAGATGC TTATTTGCGA
    GATTGTTCCA GGTCCAGCTT CGGGCTGTCC TCTGTGGCAG AGATCCCTCA GCGGGCTGAG
    AATGCAAGTC TTGCCAATTT ACAAGAGAGG AGACCGCTGA CTCTCTCACC AGTGATTGAG
    AGGATGTGCT TTGCCCACTC TAAGAAAACA CCACAGTCTC CCAAGTTTAA GGAAGTGGAT
    ATTTTTATTG GAAACAGTGG CTCTAAAAAG GAAATTGATC TTAGTAAGTA TTATCCTGAT
    GATGCAGCTG AAGAAATGAA GGCTCTTCTG GGAATATTTA ATGGCATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247789.1
    CDS211..4224
    Translation

    Target ORF information:

    RefSeq Version XM_011249487.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCAAAGT CAGACGATTG CTTCTTTTCC ATGGATAATT TGTTTTTCAG TAGCCCAGAT 
    GAAACTGAAA AACTGTCTAG AACATGTGAC AGTGAACATG ACTGTGACTA TGCTGATGGA
    AGTTTAAACT TGAGTTCTCA TATCAGCCCA GTGAAACTTA CCCAAACGAA AATAAGCAAA
    GAGAATGCAT GGACGTGTAG CAATAGTAAG CAGAAACGTC AGTATTCTAC AAACAAATTC
    AAAGCAAATG ATGCTTTTTC TGCTTCTGGA ATTGGAAAAG ACATATTCAA AGCACCATCA
    TTTCCTGCTG CATCTCAGCC TCATGATATT CAAGGAATAA CCCCAAATGG CTTAGGTTCT
    TTGAAAGCTG TCACGGAAAT CCCGGCAAAA TTTAGAAATA TATTCAAAGA ATTCCCTTAC
    TTCAACTATA TACAATCCAA AGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCCC CCACTGGTTC TGGGAAAACT GTAGTGTTTG AGCTAGCGAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGCTAAAC ATGAAGATTG TTTACATGGC ACCAATAAAA
    GCCCTTTGTA GTCAGCGGTT TGATGACTGG AAAGAAAAGT TTGGACCAGT AGGCTTGAAT
    TGTAAAGAAC TTACTGGTGA TACAGTAATG GATGACCTGT TTGAGATTCA GCATGCAAAT
    ATAATTATAA CAACTCCAGA GAAGTGGGAC AGTGTGACTA GGAAGTGGAG AGACAACTCT
    TTTATCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTGC ATGTTATTAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TTTATCTAGG
    GATTTAGAAA GTGCCAGCCC TGTTCCAGTG AGATTTGTAG CTGTGTCTGC AACAATTCCC
    AATGCTGAGG ATATCGCAGA ATGGCTTTCA GATGGTGAGA GACCTGCTGT ATGTCTCAAA
    ATGGATGAGA GCCATAGACC TGTGAAACTT CAGAAAGTAG TCCTGGGTTT TCCCTGTAGT
    AGTAGCCAAA CTGAATTTAA GTTTGATTTG GCCCTCAACT ATAAAGTGTA CAGTGTTATT
    CGAACATACT CCGATCAGAA GCCCACACTT GTGTTTTGTT CAACAAGGAA AGGTGTGCAG
    CAGGCTGCTT CTGTTCTTGT GAAGGATGCT AAGTTTATCA TCTCTGTGGA GCAGAAACTA
    CGGTTGCAGA AGTCTGCATA TTCTATAAGG GATTCAAAGC TGAAAGATAC CTTGGTATAT
    GGTGTTGGTT ACCATCATGC TGGAATGGAG CTGTCAGATA GAAAATTGGT TGAAGGATTG
    TTTACCTCTG GAGATCTACC AGTTCTTTTC ACTACCAGTA CTCTAGCTAT GGGCATGAAC
    ATGCCGGCTC ACCTGGTTGT TATAAAGTCT ACCATGCACT ACAGTGGAGG TGTGTTTGAA
    GAGTACAGCG AGACCGACAT CCTGCAGATG ATTGGGAGAG CTGGCAGACC CCAGTTTGAC
    ACTACAGCTA CTGCAGTCAT TATGACTCGA CTAAGTACAA GAGAGAAGTA CGTGCAGATG
    CTAGCTTGTA ACGACACTGT AGAGAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAAATAG TGCTGCATAC CATCACAGAC GTGAACATTG CTCTGGACTG GATCCGATCA
    ACAATGCTGT ATATCAGAGC TCTGAAAAAC CCATCTCACT ATGGTTTTTC ATCTGGATTG
    AACAAAGATG GGATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAAAGATTTA
    TCATCACTAG ACCTGATAAA GATGGATGAA GATGTTAATT TTAAACCAAC AGAGGCAGGA
    AGACTAATGG CTTGGTATTA TATTACATTT GAGACAGTCA AGAAATTTTG TGCAATCAGT
    GGAAAAGAAA CTTTATTGGA TTTAATTTCA ATGATATCGA GCTGCAATGA GTTTCTAGAT
    GTGCAGCTGA GGATAAGTGA AAAGAGAATA CTGAACACCT TAAACAAAGA CCCAAACCGG
    ATAACTATCA GATTTCCAAT GGCAGAAAGA ATTAAAACAA GAGAAATGAA AGTAAACTGT
    CTTATTCAGG CTCAGCTAGG ATGCATTCCT ATACAAGATT TTGCTTTGAC ACAAGATACA
    GTGAAGATTT TCAGAAATGG CTCACGAATT GCAAGATGGT TGTCAGATTT TGTGGCTGCT
    CAGGAAAAGA AGTTTGCTGT GCTTTTAAAC AGTGTGATTT TAACTAAATG TTTTAAGTGT
    AAACTCTGGG AAAACTCTAA ACATGTATCA AAACAGCTGG ATAAAATTGG CATATCATTG
    TCAAATACCA TGGTAAATGC AGGCTTAACT TCTTTTAAAA AAATAGAAGA AGCAAATGCA
    AGAGAACTTG AACTGATTTT AAATAGACAC CCACCATTTG GGACCCAGAT AAAAGAAGCT
    GTGGCGCATC TGCCGAAGTA TGAGCTTGAG GTGGAGCAGA TTGCAAGATA CAGTGATATC
    AAGGCAGAAA TACTGGTGAC CATTATATTA AGAAACTTTG AGCAGCTGCA AACTAAAAGA
    ACAGCACCAG ACTTCCACTA TGCCACCTTA ATCATAGGTG ATGCAGATAA CCAAGTCGTT
    TTTAAGCACA AAATTATGGA TTCTGTCTTG TTAAAAAGTG GAAATTGGGT TAAAAAAATT
    GATGTGAAGA GAGCTCTTAT ATCAGAAGAT CTCAGCATAA ATCTAATTAG TTCTGATTAT
    GTTGGTCTTG ATATTCACCA GAAATTTACA GTCTTTTATT TCGGACCCAG GAAGTTTGTA
    AATGAAACAG CTATGGAAAG AAGCTCAGAA ACAGATATTT CTCATTCTGA CTACTCAGGT
    AGAGCCACTG CAACAGGATC CAGTAAAGGA ATGGCTACCT GCAAAAAACC TGGGAACAGA
    GAATGCCATC ATCACTGTAA AAATAAACAC GCATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAAA AGCCGGAAGT TAAAGAGTCA GCAATGTCTT CATATCTGTC TGACTTAAAA
    AGCAGAGATG CTGTTTCTTC TCTTCCTCTA GCCAAGCGAC TCAAGATACA GATGAACAAA
    TCTCAAAATG TGGACCTTAA AGAGTTTGGT TTTACTCCAA GGCCTTCCCT TTCCAGTATA
    TCAAGGTCAG AATATTTAAA TACCCCTGAA TTGTCAATAT TAGAACAGAG GAATCAGCAT
    GAAATCTATG GAAAAGTTCA ACAGGGACCA TCTGAATATC GTGACAAAGA TTTTCCTAGA
    TGGGACTATT GTACAACTTC AAGTGAAAAG ACCCCTCAAA CGGTGATGTT CAGCAGGCAT
    GCTAATATAC CTGGAAACCA CAGAGTTGCA GATGAAGACA AAAGCAAGTC TTCAGAAGTT
    CTGGGTGTGA ACTTAGAGCT GGGAAATGAA GTCTGGGATG ACTTTGATGA TGAGAGCTTA
    ATAGAAGTTA TGAGCCTTTC AGCTGATGCT GAGAAGATGG CTGCATCAGG ATTTGGAGAC
    ACTCGTGATT CAAGTCTCGG CGGAAGTAAG CTACCCTTCC AAAAGTCAAG CAGCAGATTC
    CAAAGAGACA ACTCAAACAG TTTTGCTTCG TCGCCTGGGA AGCCAGATGC TTATTTGCGA
    GATTGTTCCA GGTCCAGCTT CGGGCTGTCC TCTGTGGCAG AGATCCCTCA GCGGGCTGAG
    AATGCAAGTC TTGCCAATTT ACAAGAGAGG AGACCGCTGA CTCTCTCACC AGTGATTGAG
    AGGATGTGCT TTGCCCACTC TAAGAAAACA CCACAGTCTC CCAAGTTTAA GGAAGTGGAT
    ATTTTTATTG GAAACAGTGG CTCTAAAAAG GAAATTGATC TTAGTAAGTA TTATCCTGAT
    GATGCAGCTG AAGAAATGAA GGCTCTTCTG GGAATATTTA ATGGCATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.