Hfm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hfm1
Entrez Gene ID	690161
Full Name	HFM1, ATP-dependent DNA helicase homolog
General protein information	Preferred Names HFM1, ATP-dependent DNA helicase homolog Names probable ATP-dependent DNA helicase HFM1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769948.2	XP_008768170.1	probable ATP-dependent DNA helicase HFM1 isoform X5
XM_008769943.2	XP_008768165.1	probable ATP-dependent DNA helicase HFM1 isoform X1
XM_008769945.2	XP_008768167.1	probable ATP-dependent DNA helicase HFM1 isoform X2
XM_006250582.3	XP_006250644.1	probable ATP-dependent DNA helicase HFM1 isoform X1
XM_017599385.1	XP_017454874.1	probable ATP-dependent DNA helicase HFM1 isoform X3
XM_008769947.2	XP_008768169.1	probable ATP-dependent DNA helicase HFM1 isoform X4
NM_001191101.1	NP_001178030.1	probable ATP-dependent DNA helicase HFM1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HFM1	NP_001017975.3
Danio rerio (zebrafish)	hfm1	XP_001919555.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009060	XP_552976.3
Arabidopsis thaliana (thale cress)	RCK	NP_189410.2
Xenopus tropicalis (tropical clawed frog)	hfm1	NP_001072383.3
Pan troglodytes (chimpanzee)	HFM1	XP_524618.3
Oryza sativa (rice)	Os02g0617500	NP_001047442.1
Rattus norvegicus (Norway rat)	Hfm1	NP_001178030.1
	LOC704516	XP_002808271.1
Canis lupus familiaris (dog)	HFM1	XP_005621996.1
Bos taurus (cattle)	HFM1	NP_001192505.1
Mus musculus (house mouse)	Hfm1	NP_808541.2
Gallus gallus (chicken)	HFM1	XP_422349.3

Gene Ontology
Bibliography

Related articles in PubMed

Mouse HFM1/Mer3 is required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
Guiraldelli MF, Eyster C, Wilkerson JL, Dresser ME, Pezza RJ
PLoS genetics9(3)e1003383(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hfm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hfm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06894	NM_001191101.1 Latest version!	Rattus norvegicus helicase for meiosis 1 (Hfm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa35812	XM_008769943.2 Latest version!	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35812	XM_006250582.3 Latest version!	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa50446	XM_008769945.2 Latest version!	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa50447	XM_017599385.1 Latest version!	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa50448	XM_008769947.2 Latest version!	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa50449	XM_008769948.2 Latest version!	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hfm1 ( NM_001191101.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178030.1 Rattus norvegicus helicase for meiosis 1 (Hfm1), mRNA.

Price & Availability↑

CloneID	ORa06894
Clone ID Related Accession (Same CDS sequence)	NM_001191101.1
Accession Version	NM_001191101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-15
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR07014161.1. On Jul 17, 2010 this sequence version replaced XM_001073516.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGACGG CCCACATGTG 
AACTCAGAGC TGGGAGATGA AGTTTGGGAT GACTTTGATG ATGAAAGCTT AATAGAAGTT 
ATGAGCTTTT CAGCTGACGC CGAGGAGATG GCTGTGTCAG GGTTTGGAGA CACTCGTGAT 
TCAAGTCTAG GCAGAAGTAA GCTACACTTC GGAAGATCAA GCAGCAGGTT CCAAAGAGAC 
ATCTCAAACA GTTTTGTTTC ATCACCCGAG AAGCCAGATG TTTGTTTGTC AGATTGTTCC 
GTGTCTAATT TCGGGCTGTC CTCTGTGGCA GAGTTCCCTC AACAAGCTGG AAATGCAAGT 
CTTGTCAATT TACAAGAGAG AAAACAGCTG ACCCTCTCAC CAGTGATTGA GAGGATGTGC 
TTTACCCACT CTGAGAAAAT ACCACAGTCT CCCAGTTTTA AGGAAGTGGA TATTTTTATT 
GGAAACAGTG ACTCTAAAAA GGAAATTGAT CTTAGTAAGT ATTATCCTGA TGATGCAGCC 
GAGGAAATGA AGGCCCTTCT GGGAATATTT AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178030.1
CDS	1..4305
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	NM_001191101.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus helicase for meiosis 1 (Hfm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191101.1

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGACGG CCCACATGTG 
AACTCAGAGC TGGGAGATGA AGTTTGGGAT GACTTTGATG ATGAAAGCTT AATAGAAGTT 
ATGAGCTTTT CAGCTGACGC CGAGGAGATG GCTGTGTCAG GGTTTGGAGA CACTCGTGAT 
TCAAGTCTAG GCAGAAGTAA GCTACACTTC GGAAGATCAA GCAGCAGGTT CCAAAGAGAC 
ATCTCAAACA GTTTTGTTTC ATCACCCGAG AAGCCAGATG TTTGTTTGTC AGATTGTTCC 
GTGTCTAATT TCGGGCTGTC CTCTGTGGCA GAGTTCCCTC AACAAGCTGG AAATGCAAGT 
CTTGTCAATT TACAAGAGAG AAAACAGCTG ACCCTCTCAC CAGTGATTGA GAGGATGTGC 
TTTACCCACT CTGAGAAAAT ACCACAGTCT CCCAGTTTTA AGGAAGTGGA TATTTTTATT 
GGAAACAGTG ACTCTAAAAA GGAAATTGAT CTTAGTAAGT ATTATCCTGA TGATGCAGCC 
GAGGAAATGA AGGCCCTTCT GGGAATATTT AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hfm1 ( XM_008769943.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768165.1 Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35812
Clone ID Related Accession (Same CDS sequence)	XM_008769943.2 , XM_006250582.3
Accession Version	XM_008769943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTGG AGAATGGACG 
TTCTGTCACT GTAGCCCCAG GGAAAGCAGT GCCACGAAAA ATGACCGCAC AGGGATCACC 
TATTGTGACA ATGCAGACTT TCCCGGATGG GACTATTGTG CAGCTTCAAA CAAAAGGACC 
CCAGTGATGC TCAGCAGACA TGCTAACACA CCTGGAAACC ACGGTGTCGC AGTTGCAGAG 
AAGAGCAAGT CTTCAGACGG CCCACATGTG AACTCAGAGC TGGGAGATGA AGTTTGGGAT 
GACTTTGATG ATGAAAGCTT AATAGAAGTT ATGAGCTTTT CAGCTGACGC CGAGGAGATG 
GCTGTGTCAG GGTTTGGAGA CACTCGTGAT TCAAGTCTAG GCAGAAGTAA GCTACACTTC 
GGAAGATCAA GCAGCAGGTT CCAAAGAGAC ATCTCAAACA GTTTTGTTTC ATCACCCGAG 
AAGCCAGATG TTTGTTTGTC AGATTGTTCC GTGTCTAATT TCGGGCTGTC CTCTGTGGCA 
GAGTTCCCTC AACAAGCTGG AAATGCAAGT CTTGTCAATT TACAAGAGAG AAAACAGCTG 
ACCCTCTCAC CAGTGATTGA GAGGATGTGC TTTACCCACT CTGAGAAAAT ACCACAGTCT 
CCCAGTTTTA AGGAAGTGGA TATTTTTATT GGAAACAGTG ACTCTAAAAA GGAAATTGAT 
CTTAGTAAGT ATTATCCTGA TGATGCAGCC GAGGAAATGA AGGCCCTTCT GGGAATATTT 
AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768165.1
CDS	118..4632
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEAGEWTFCHCSPRESSATKNDRTGITYCDNADFPGWDYCAASNKRTPVMLSRHANTPGNHGVAVAEKSKSSDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	XM_008769943.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769943.2

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTGG AGAATGGACG 
TTCTGTCACT GTAGCCCCAG GGAAAGCAGT GCCACGAAAA ATGACCGCAC AGGGATCACC 
TATTGTGACA ATGCAGACTT TCCCGGATGG GACTATTGTG CAGCTTCAAA CAAAAGGACC 
CCAGTGATGC TCAGCAGACA TGCTAACACA CCTGGAAACC ACGGTGTCGC AGTTGCAGAG 
AAGAGCAAGT CTTCAGACGG CCCACATGTG AACTCAGAGC TGGGAGATGA AGTTTGGGAT 
GACTTTGATG ATGAAAGCTT AATAGAAGTT ATGAGCTTTT CAGCTGACGC CGAGGAGATG 
GCTGTGTCAG GGTTTGGAGA CACTCGTGAT TCAAGTCTAG GCAGAAGTAA GCTACACTTC 
GGAAGATCAA GCAGCAGGTT CCAAAGAGAC ATCTCAAACA GTTTTGTTTC ATCACCCGAG 
AAGCCAGATG TTTGTTTGTC AGATTGTTCC GTGTCTAATT TCGGGCTGTC CTCTGTGGCA 
GAGTTCCCTC AACAAGCTGG AAATGCAAGT CTTGTCAATT TACAAGAGAG AAAACAGCTG 
ACCCTCTCAC CAGTGATTGA GAGGATGTGC TTTACCCACT CTGAGAAAAT ACCACAGTCT 
CCCAGTTTTA AGGAAGTGGA TATTTTTATT GGAAACAGTG ACTCTAAAAA GGAAATTGAT 
CTTAGTAAGT ATTATCCTGA TGATGCAGCC GAGGAAATGA AGGCCCTTCT GGGAATATTT 
AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hfm1 ( XM_006250582.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250644.1 Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X2, mRNA.

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CloneID	ORa35812
Clone ID Related Accession (Same CDS sequence)	XM_008769943.2 , XM_006250582.3
Accession Version	XM_006250582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTGG AGAATGGACG 
TTCTGTCACT GTAGCCCCAG GGAAAGCAGT GCCACGAAAA ATGACCGCAC AGGGATCACC 
TATTGTGACA ATGCAGACTT TCCCGGATGG GACTATTGTG CAGCTTCAAA CAAAAGGACC 
CCAGTGATGC TCAGCAGACA TGCTAACACA CCTGGAAACC ACGGTGTCGC AGTTGCAGAG 
AAGAGCAAGT CTTCAGACGG CCCACATGTG AACTCAGAGC TGGGAGATGA AGTTTGGGAT 
GACTTTGATG ATGAAAGCTT AATAGAAGTT ATGAGCTTTT CAGCTGACGC CGAGGAGATG 
GCTGTGTCAG GGTTTGGAGA CACTCGTGAT TCAAGTCTAG GCAGAAGTAA GCTACACTTC 
GGAAGATCAA GCAGCAGGTT CCAAAGAGAC ATCTCAAACA GTTTTGTTTC ATCACCCGAG 
AAGCCAGATG TTTGTTTGTC AGATTGTTCC GTGTCTAATT TCGGGCTGTC CTCTGTGGCA 
GAGTTCCCTC AACAAGCTGG AAATGCAAGT CTTGTCAATT TACAAGAGAG AAAACAGCTG 
ACCCTCTCAC CAGTGATTGA GAGGATGTGC TTTACCCACT CTGAGAAAAT ACCACAGTCT 
CCCAGTTTTA AGGAAGTGGA TATTTTTATT GGAAACAGTG ACTCTAAAAA GGAAATTGAT 
CTTAGTAAGT ATTATCCTGA TGATGCAGCC GAGGAAATGA AGGCCCTTCT GGGAATATTT 
AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250644.1
CDS	83..4597
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEAGEWTFCHCSPRESSATKNDRTGITYCDNADFPGWDYCAASNKRTPVMLSRHANTPGNHGVAVAEKSKSSDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	XM_006250582.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250582.3

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTGG AGAATGGACG 
TTCTGTCACT GTAGCCCCAG GGAAAGCAGT GCCACGAAAA ATGACCGCAC AGGGATCACC 
TATTGTGACA ATGCAGACTT TCCCGGATGG GACTATTGTG CAGCTTCAAA CAAAAGGACC 
CCAGTGATGC TCAGCAGACA TGCTAACACA CCTGGAAACC ACGGTGTCGC AGTTGCAGAG 
AAGAGCAAGT CTTCAGACGG CCCACATGTG AACTCAGAGC TGGGAGATGA AGTTTGGGAT 
GACTTTGATG ATGAAAGCTT AATAGAAGTT ATGAGCTTTT CAGCTGACGC CGAGGAGATG 
GCTGTGTCAG GGTTTGGAGA CACTCGTGAT TCAAGTCTAG GCAGAAGTAA GCTACACTTC 
GGAAGATCAA GCAGCAGGTT CCAAAGAGAC ATCTCAAACA GTTTTGTTTC ATCACCCGAG 
AAGCCAGATG TTTGTTTGTC AGATTGTTCC GTGTCTAATT TCGGGCTGTC CTCTGTGGCA 
GAGTTCCCTC AACAAGCTGG AAATGCAAGT CTTGTCAATT TACAAGAGAG AAAACAGCTG 
ACCCTCTCAC CAGTGATTGA GAGGATGTGC TTTACCCACT CTGAGAAAAT ACCACAGTCT 
CCCAGTTTTA AGGAAGTGGA TATTTTTATT GGAAACAGTG ACTCTAAAAA GGAAATTGAT 
CTTAGTAAGT ATTATCCTGA TGATGCAGCC GAGGAAATGA AGGCCCTTCT GGGAATATTT 
AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hfm1 ( XM_008769945.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768167.1 Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA.

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CloneID	ORa50446
Clone ID Related Accession (Same CDS sequence)	XM_008769945.2
Accession Version	XM_008769945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTGG AGAATGGACG 
TTCTGTCACT GTAGCCCCAG GGAAAGCAGT GCCACGAAAA ATGACCGCAC AGGGATCACC 
TATTGTGACA ATGACTTTCC CGGATGGGAC TATTGTGCAG CTTCAAACAA AAGGACCCCA 
GTGATGCTCA GCAGACATGC TAACACACCT GGAAACCACG GTGTCGCAGT TGCAGAGAAG 
AGCAAGTCTT CAGACGGCCC ACATGTGAAC TCAGAGCTGG GAGATGAAGT TTGGGATGAC 
TTTGATGATG AAAGCTTAAT AGAAGTTATG AGCTTTTCAG CTGACGCCGA GGAGATGGCT 
GTGTCAGGGT TTGGAGACAC TCGTGATTCA AGTCTAGGCA GAAGTAAGCT ACACTTCGGA 
AGATCAAGCA GCAGGTTCCA AAGAGACATC TCAAACAGTT TTGTTTCATC ACCCGAGAAG 
CCAGATGTTT GTTTGTCAGA TTGTTCCGTG TCTAATTTCG GGCTGTCCTC TGTGGCAGAG 
TTCCCTCAAC AAGCTGGAAA TGCAAGTCTT GTCAATTTAC AAGAGAGAAA ACAGCTGACC 
CTCTCACCAG TGATTGAGAG GATGTGCTTT ACCCACTCTG AGAAAATACC ACAGTCTCCC 
AGTTTTAAGG AAGTGGATAT TTTTATTGGA AACAGTGACT CTAAAAAGGA AATTGATCTT 
AGTAAGTATT ATCCTGATGA TGCAGCCGAG GAAATGAAGG CCCTTCTGGG AATATTTAAT 
GGCATTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768167.1
CDS	283..4794
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEAGEWTFCHCSPRESSATKNDRTGITYCDNDFPGWDYCAASNKRTPVMLSRHANTPGNHGVAVAEKSKSSDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	XM_008769945.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769945.2

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTGG AGAATGGACG 
TTCTGTCACT GTAGCCCCAG GGAAAGCAGT GCCACGAAAA ATGACCGCAC AGGGATCACC 
TATTGTGACA ATGACTTTCC CGGATGGGAC TATTGTGCAG CTTCAAACAA AAGGACCCCA 
GTGATGCTCA GCAGACATGC TAACACACCT GGAAACCACG GTGTCGCAGT TGCAGAGAAG 
AGCAAGTCTT CAGACGGCCC ACATGTGAAC TCAGAGCTGG GAGATGAAGT TTGGGATGAC 
TTTGATGATG AAAGCTTAAT AGAAGTTATG AGCTTTTCAG CTGACGCCGA GGAGATGGCT 
GTGTCAGGGT TTGGAGACAC TCGTGATTCA AGTCTAGGCA GAAGTAAGCT ACACTTCGGA 
AGATCAAGCA GCAGGTTCCA AAGAGACATC TCAAACAGTT TTGTTTCATC ACCCGAGAAG 
CCAGATGTTT GTTTGTCAGA TTGTTCCGTG TCTAATTTCG GGCTGTCCTC TGTGGCAGAG 
TTCCCTCAAC AAGCTGGAAA TGCAAGTCTT GTCAATTTAC AAGAGAGAAA ACAGCTGACC 
CTCTCACCAG TGATTGAGAG GATGTGCTTT ACCCACTCTG AGAAAATACC ACAGTCTCCC 
AGTTTTAAGG AAGTGGATAT TTTTATTGGA AACAGTGACT CTAAAAAGGA AATTGATCTT 
AGTAAGTATT ATCCTGATGA TGCAGCCGAG GAAATGAAGG CCCTTCTGGG AATATTTAAT 
GGCATTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hfm1 ( XM_017599385.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454874.1 Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa50447
Clone ID Related Accession (Same CDS sequence)	XM_017599385.1
Accession Version	XM_017599385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCAGA CTTTCCCGGA 
TGGGACTATT GTGCAGCTTC AAACAAAAGG ACCCCAGTGA TGCTCAGCAG ACATGCTAAC 
ACACCTGGAA ACCACGGTGT CGCAGTTGCA GAGAAGAGCA AGTCTTCAGA CGGCCCACAT 
GTGAACTCAG AGCTGGGAGA TGAAGTTTGG GATGACTTTG ATGATGAAAG CTTAATAGAA 
GTTATGAGCT TTTCAGCTGA CGCCGAGGAG ATGGCTGTGT CAGGGTTTGG AGACACTCGT 
GATTCAAGTC TAGGCAGAAG TAAGCTACAC TTCGGAAGAT CAAGCAGCAG GTTCCAAAGA 
GACATCTCAA ACAGTTTTGT TTCATCACCC GAGAAGCCAG ATGTTTGTTT GTCAGATTGT 
TCCGTGTCTA ATTTCGGGCT GTCCTCTGTG GCAGAGTTCC CTCAACAAGC TGGAAATGCA 
AGTCTTGTCA ATTTACAAGA GAGAAAACAG CTGACCCTCT CACCAGTGAT TGAGAGGATG 
TGCTTTACCC ACTCTGAGAA AATACCACAG TCTCCCAGTT TTAAGGAAGT GGATATTTTT 
ATTGGAAACA GTGACTCTAA AAAGGAAATT GATCTTAGTA AGTATTATCC TGATGATGCA 
GCCGAGGAAA TGAAGGCCCT TCTGGGAATA TTTAATGGCA TTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454874.1
CDS	248..4675
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEADFPGWDYCAASNKRTPVMLSRHANTPGNHGVAVAEKSKSSDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	XM_017599385.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599385.1

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCAGA CTTTCCCGGA 
TGGGACTATT GTGCAGCTTC AAACAAAAGG ACCCCAGTGA TGCTCAGCAG ACATGCTAAC 
ACACCTGGAA ACCACGGTGT CGCAGTTGCA GAGAAGAGCA AGTCTTCAGA CGGCCCACAT 
GTGAACTCAG AGCTGGGAGA TGAAGTTTGG GATGACTTTG ATGATGAAAG CTTAATAGAA 
GTTATGAGCT TTTCAGCTGA CGCCGAGGAG ATGGCTGTGT CAGGGTTTGG AGACACTCGT 
GATTCAAGTC TAGGCAGAAG TAAGCTACAC TTCGGAAGAT CAAGCAGCAG GTTCCAAAGA 
GACATCTCAA ACAGTTTTGT TTCATCACCC GAGAAGCCAG ATGTTTGTTT GTCAGATTGT 
TCCGTGTCTA ATTTCGGGCT GTCCTCTGTG GCAGAGTTCC CTCAACAAGC TGGAAATGCA 
AGTCTTGTCA ATTTACAAGA GAGAAAACAG CTGACCCTCT CACCAGTGAT TGAGAGGATG 
TGCTTTACCC ACTCTGAGAA AATACCACAG TCTCCCAGTT TTAAGGAAGT GGATATTTTT 
ATTGGAAACA GTGACTCTAA AAAGGAAATT GATCTTAGTA AGTATTATCC TGATGATGCA 
GCCGAGGAAA TGAAGGCCCT TCTGGGAATA TTTAATGGCA TTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hfm1 ( XM_008769947.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768169.1 Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa50448
Clone ID Related Accession (Same CDS sequence)	XM_008769947.2
Accession Version	XM_008769947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGACTT TCCCGGATGG 
GACTATTGTG CAGCTTCAAA CAAAAGGACC CCAGTGATGC TCAGCAGACA TGCTAACACA 
CCTGGAAACC ACGGTGTCGC AGTTGCAGAG AAGAGCAAGT CTTCAGACGG CCCACATGTG 
AACTCAGAGC TGGGAGATGA AGTTTGGGAT GACTTTGATG ATGAAAGCTT AATAGAAGTT 
ATGAGCTTTT CAGCTGACGC CGAGGAGATG GCTGTGTCAG GGTTTGGAGA CACTCGTGAT 
TCAAGTCTAG GCAGAAGTAA GCTACACTTC GGAAGATCAA GCAGCAGGTT CCAAAGAGAC 
ATCTCAAACA GTTTTGTTTC ATCACCCGAG AAGCCAGATG TTTGTTTGTC AGATTGTTCC 
GTGTCTAATT TCGGGCTGTC CTCTGTGGCA GAGTTCCCTC AACAAGCTGG AAATGCAAGT 
CTTGTCAATT TACAAGAGAG AAAACAGCTG ACCCTCTCAC CAGTGATTGA GAGGATGTGC 
TTTACCCACT CTGAGAAAAT ACCACAGTCT CCCAGTTTTA AGGAAGTGGA TATTTTTATT 
GGAAACAGTG ACTCTAAAAA GGAAATTGAT CTTAGTAAGT ATTATCCTGA TGATGCAGCC 
GAGGAAATGA AGGCCCTTCT GGGAATATTT AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768169.1
CDS	247..4671
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEDFPGWDYCAASNKRTPVMLSRHANTPGNHGVAVAEKSKSSDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	XM_008769947.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769947.2

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGACTT TCCCGGATGG 
GACTATTGTG CAGCTTCAAA CAAAAGGACC CCAGTGATGC TCAGCAGACA TGCTAACACA 
CCTGGAAACC ACGGTGTCGC AGTTGCAGAG AAGAGCAAGT CTTCAGACGG CCCACATGTG 
AACTCAGAGC TGGGAGATGA AGTTTGGGAT GACTTTGATG ATGAAAGCTT AATAGAAGTT 
ATGAGCTTTT CAGCTGACGC CGAGGAGATG GCTGTGTCAG GGTTTGGAGA CACTCGTGAT 
TCAAGTCTAG GCAGAAGTAA GCTACACTTC GGAAGATCAA GCAGCAGGTT CCAAAGAGAC 
ATCTCAAACA GTTTTGTTTC ATCACCCGAG AAGCCAGATG TTTGTTTGTC AGATTGTTCC 
GTGTCTAATT TCGGGCTGTC CTCTGTGGCA GAGTTCCCTC AACAAGCTGG AAATGCAAGT 
CTTGTCAATT TACAAGAGAG AAAACAGCTG ACCCTCTCAC CAGTGATTGA GAGGATGTGC 
TTTACCCACT CTGAGAAAAT ACCACAGTCT CCCAGTTTTA AGGAAGTGGA TATTTTTATT 
GGAAACAGTG ACTCTAAAAA GGAAATTGAT CTTAGTAAGT ATTATCCTGA TGATGCAGCC 
GAGGAAATGA AGGCCCTTCT GGGAATATTT AATGGCATTT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hfm1 ( XM_008769948.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768170.1 Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA.

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CloneID	ORa50449
Clone ID Related Accession (Same CDS sequence)	XM_008769948.2
Accession Version	XM_008769948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent DNA helicase HFM1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTTC AAACAAAAGG 
ACCCCAGTGA TGCTCAGCAG ACATGCTAAC ACACCTGGAA ACCACGGTGT CGCAGTTGCA 
GAGAAGAGCA AGTCTTCAGA CGGCCCACAT GTGAACTCAG AGCTGGGAGA TGAAGTTTGG 
GATGACTTTG ATGATGAAAG CTTAATAGAA GTTATGAGCT TTTCAGCTGA CGCCGAGGAG 
ATGGCTGTGT CAGGGTTTGG AGACACTCGT GATTCAAGTC TAGGCAGAAG TAAGCTACAC 
TTCGGAAGAT CAAGCAGCAG GTTCCAAAGA GACATCTCAA ACAGTTTTGT TTCATCACCC 
GAGAAGCCAG ATGTTTGTTT GTCAGATTGT TCCGTGTCTA ATTTCGGGCT GTCCTCTGTG 
GCAGAGTTCC CTCAACAAGC TGGAAATGCA AGTCTTGTCA ATTTACAAGA GAGAAAACAG 
CTGACCCTCT CACCAGTGAT TGAGAGGATG TGCTTTACCC ACTCTGAGAA AATACCACAG 
TCTCCCAGTT TTAAGGAAGT GGATATTTTT ATTGGAAACA GTGACTCTAA AAAGGAAATT 
GATCTTAGTA AGTATTATCC TGATGATGCA GCCGAGGAAA TGAAGGCCCT TCTGGGAATA 
TTTAATGGCA TTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768170.1
CDS	247..4644
Translation	MLESNDCILSMDSLFFSSPGETESFPVEEKSLDWFLPPAPLISEIPDIQELEEEIENYKLLGERKMPNILTSNLKIINEDTNCISPTQKFHFPYNIHEQDYLNLGGLNNNDMSHTAGKLVYGSSQKYKNHMGDESPPTKSGPGDAKLHTAAEDREGTSALKTRLSRTCGSVQECDYANGNLSLNSHISPARLTQTKVSKEKPWNCSNRKQKLQYSTDKSKANDAFSASGIEKDIFEAPSFSAASQPRDIQGITSNGLGSLKAVTEIPAKFRSVFKEFPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEAPLPWLNMKVVYMAPIKALCSQRFDDWKEKFGPIGLTCKELTGDTVMDDLFEIQHANIIMTTPEKWDSMTRKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSKTLENASPAPVRFVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPSSSNQTEFKFDLALNYKVYSVIRTYSDQKPTLVFCATRKGVQQAASVLVKDAKFIITVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEELFTSGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYTGGAFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLACNDTVESSLHRHLIEHLNAEIVLHTITDVSIALDWIRSTLLYIRALKNPSHYGFISGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDLNFRSTEAGRLMAWYYITFETVKKFCAISGKETLSDLISMISSCSEFLDVQLRISEKRTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTSKIFRNGSRIAKWLSDFVTAQEQKFAVLLNSVILAKCFKCKLWENSQHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVMYLPKYELEAEQIARYSDTKAEILVTIILRNFEQLQTKRTAPDFHYATLIIGDADNQVVCKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKFTVFYLGPRKFVNQTVTERSSGTGLSHSECSDRATAAGSSKRTATCRNPGNRECHHHCKNKHICGHDCCKIGVAQKPEAKVSAVSSYLSDLKSRDAVSSFPPAKRLKIQMNKSQNVDLKEFGFVPRPSLSSIARSEYLNIHKLPILEQRTQHEIYGEVEEGASECRDEASNKRTPVMLSRHANTPGNHGVAVAEKSKSSDGPHVNSELGDEVWDDFDDESLIEVMSFSADAEEMAVSGFGDTRDSSLGRSKLHFGRSSSRFQRDISNSFVSSPEKPDVCLSDCSVSNFGLSSVAEFPQQAGNASLVNLQERKQLTLSPVIERMCFTHSEKIPQSPSFKEVDIFIGNSDSKKEIDLSKYYPDDAAEEMKALLGIFNGIF

Target ORF information:

RefSeq Version	XM_008769948.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HFM1, ATP-dependent DNA helicase homolog (Hfm1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769948.2

ORF Insert Sequence:

ATGCTGGAAT CAAACGATTG CATTTTATCC ATGGATTCTT TGTTTTTCAG TAGCCCAGGT 
GAAACTGAAA GCTTTCCAGT TGAGGAAAAA TCACTGGATT GGTTTCTCCC TCCAGCTCCA 
CTGATCTCAG AAATTCCAGA TATTCAAGAG TTAGAGGAAG AAATTGAAAA TTATAAGCTA 
TTAGGAGAAA GAAAGATGCC CAATATATTA ACATCAAACT TGAAGATCAT TAATGAAGAC 
ACAAATTGTA TTTCACCAAC ACAGAAATTC CATTTTCCCT ATAACATACA TGAACAGGAT 
TACCTAAATT TAGGAGGATT AAATAACAAT GACATGTCAC ATACAGCTGG CAAGCTAGTG 
TACGGTTCTT CTCAAAAATA TAAAAACCAC ATGGGGGATG AGAGTCCACC GACAAAGAGT 
GGTCCTGGTG ATGCAAAGCT GCATACTGCT GCTGAGGACA GAGAGGGCAC ATCAGCACTC 
AAAACCAGAT TATCTAGAAC ATGTGGCAGT GTGCAGGAAT GTGACTATGC TAATGGAAAT 
TTAAGCTTGA ATTCTCATAT TAGCCCAGCG AGACTCACCC AAACAAAAGT AAGCAAAGAG 
AAACCATGGA ACTGTAGCAA TAGAAAACAG AAACTGCAGT ATTCTACAGA CAAATCCAAA 
GCAAACGATG CTTTTTCTGC TTCTGGAATT GAAAAAGACA TATTCGAAGC ACCATCGTTT 
TCTGCTGCAT CTCAGCCTCG TGACATTCAA GGCATAACCT CAAATGGCTT AGGTTCGTTG 
AAGGCTGTCA CAGAAATCCC TGCAAAATTT AGAAGTGTAT TCAAAGAATT CCCTTACTTC 
AACTATATAC AGTCCAAAGC CTTTGATGAT CTTCTTTACA CAGATCGGAA TTTTGTGATT 
TGTGCCCCCA CTGGTTCTGG GAAAACTGTG GTGTTTGAGC TAGCAATAAC AAGATTGTTA 
ATGGAAGCAC CATTGCCGTG GCTGAACATG AAAGTTGTTT ACATGGCACC AATAAAAGCC 
CTTTGTAGTC AGCGGTTTGA TGACTGGAAA GAAAAATTTG GACCGATAGG CTTGACCTGT 
AAAGAACTTA CTGGAGATAC AGTAATGGAT GACCTGTTTG AGATTCAGCA TGCCAATATA 
ATTATGACAA CTCCAGAGAA GTGGGACAGT ATGACTAGGA AGTGGAGAGA CAACTCCTTC 
ATCCAACTGG TTCGCCTGTT TCTCATTGAT GAGGTGCATG TTATTAAAGA TGAAAACCGT 
GGTCCAACTC TTGAGGTTGT AGTAAGCAGA ATGAAAACTG TCCAGTCTTT ATCTAAGACC 
TTAGAAAACG CCAGCCCTGC TCCAGTGAGG TTTGTAGCTG TATCAGCAAC AATTCCCAAT 
GCTGAGGATA TCGCAGAATG GCTTTCAGAT GGGGAGAGAC CTGCTGTGTG TCTGAAAATG 
GATGAGAGCC ACAGACCTGT GAAACTTCAG AAAGTGGTCC TGGGTTTCCC CAGTAGTAGT 
AACCAAACTG AATTCAAGTT TGATTTAGCC CTCAACTATA AAGTGTACAG TGTTATACGA 
ACGTACTCCG ATCAGAAGCC CACACTCGTG TTTTGTGCAA CGAGGAAAGG TGTGCAGCAG 
GCTGCTTCTG TTCTTGTGAA GGACGCTAAG TTTATCATCA CCGTGGAGCA GAAACTACGG 
TTGCAGAAGT CTGCATATTC TATAAGGGAT TCAAAGCTAA AAGATACCTT GGTGTACGGT 
GTTGGTTACC ATCATGCTGG AATGGAGCTG TCAGATAGAA AATTGGTTGA AGAGTTGTTT 
ACTTCTGGAG ATCTACCAGT TCTTTTTACT ACAAGTACTC TAGCTATGGG AGTGAACTTG 
CCGGCTCACC TGGTCGTTAT AAAGTCCACC ATGCACTACA CTGGAGGTGC CTTTGAAGAG 
TACAGCGAGA CAGACATCCT GCAGATGATT GGAAGAGCTG GCAGGCCCCA GTTTGACACC 
ACAGCTACTG CAGTTATTAT GACCCGACTA AGTACAAGAG AGAAGTACGT TCAGATGCTA 
GCTTGTAATG ACACTGTGGA AAGCAGTTTG CACAGACATC TTATTGAGCA CCTGAACGCA 
GAGATAGTGC TGCACACCAT CACAGATGTG AGCATCGCCC TGGACTGGAT CCGGTCCACA 
CTGCTGTATA TCAGAGCTCT GAAAAACCCA TCTCACTATG GTTTTATATC TGGATTGAAC 
AAAGATGGAA TTGAAGCAAA ATTACAAGAG TTATGTTTGA AGAATCTGAA AGATTTATCA 
TCGCTAGACC TAATAAAGAT GGATGAAGAC CTTAATTTCA GATCAACAGA GGCAGGAAGA 
CTAATGGCAT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTGTGC AATCAGTGGA 
AAAGAAACTT TATCAGATTT GATTTCAATG ATATCCAGCT GCAGTGAGTT TCTAGATGTG 
CAGCTGAGGA TAAGCGAAAA GAGAACACTG AACACACTAA ACAAAGACCC AAACCGGATA 
ACCATCAGAT TTCCAATGGA GGGGAGAATT AAAACCAGAG AGATGAAAGT AAACTGTCTT 
ATTCAGGCTC AGCTAGGATG CATCCCTATA CAAGATTTTG CTTTGACACA AGATACCTCG 
AAGATTTTCA GAAATGGCTC ACGAATTGCA AAATGGTTGT CAGATTTTGT GACTGCTCAG 
GAACAGAAGT TTGCTGTGCT TTTAAACAGT GTGATTTTAG CTAAATGTTT TAAGTGTAAA 
CTCTGGGAAA ACTCTCAACA TGTTTCAAAA CAGCTGGATA AAATTGGCAT ATCATTGTCA 
AATACCATGG TAAATGCAGG CTTGACGTCT TTTAAAAAAA TAGAAGAAGC AAATGCGAGA 
GAACTTGAAC TGATTTTAAA TAGACACCCG CCATTCGGGA CCCAGATAAA GGAAGCTGTG 
ATGTATCTGC CAAAGTATGA ACTTGAGGCA GAGCAGATTG CAAGATATAG TGATACCAAG 
GCAGAAATAC TGGTGACCAT TATATTAAGA AACTTTGAGC AGCTGCAGAC CAAAAGAACA 
GCGCCAGACT TTCACTATGC CACCTTAATC ATAGGTGATG CAGATAACCA AGTCGTTTGT 
AAGCACAAAA TTATGGATTC TGTCTTGTTA AAAAGTGGAA ATTGGGTTAA AAAAATTGAT 
GTGAAGAGAG CTCTTATATC AGAAGATCTC AGCATAAATC TAATTAGCTC TGATTATGTT 
GGTCTTGATA TTCACCAGAA ATTTACAGTC TTTTATTTAG GACCCAGGAA GTTTGTAAAT 
CAAACAGTTA CGGAAAGAAG CTCAGGAACA GGTCTTTCTC ATTCCGAATG CTCAGACAGA 
GCTACTGCAG CAGGATCCAG TAAAAGAACG GCTACCTGCA GAAACCCTGG GAACCGAGAA 
TGCCATCATC ACTGTAAAAA TAAGCACATA TGTGGGCATG ACTGCTGTAA AATTGGAGTT 
GCACAAAAGC CAGAAGCTAA AGTGTCAGCA GTGTCCTCAT ATCTGTCTGA CCTAAAAAGC 
AGAGATGCTG TTTCTTCTTT TCCCCCAGCT AAGCGACTCA AGATACAGAT GAACAAATCT 
CAAAATGTGG ACCTTAAAGA ATTTGGTTTT GTTCCAAGGC CTTCCCTTTC CAGTATAGCA 
AGGTCAGAAT ATTTAAACAT CCATAAATTG CCAATATTAG AACAGAGGAC TCAGCATGAG 
ATCTATGGCG AAGTTGAAGA GGGAGCGTCT GAATGCCGAG ACGAAGCTTC AAACAAAAGG 
ACCCCAGTGA TGCTCAGCAG ACATGCTAAC ACACCTGGAA ACCACGGTGT CGCAGTTGCA 
GAGAAGAGCA AGTCTTCAGA CGGCCCACAT GTGAACTCAG AGCTGGGAGA TGAAGTTTGG 
GATGACTTTG ATGATGAAAG CTTAATAGAA GTTATGAGCT TTTCAGCTGA CGCCGAGGAG 
ATGGCTGTGT CAGGGTTTGG AGACACTCGT GATTCAAGTC TAGGCAGAAG TAAGCTACAC 
TTCGGAAGAT CAAGCAGCAG GTTCCAAAGA GACATCTCAA ACAGTTTTGT TTCATCACCC 
GAGAAGCCAG ATGTTTGTTT GTCAGATTGT TCCGTGTCTA ATTTCGGGCT GTCCTCTGTG 
GCAGAGTTCC CTCAACAAGC TGGAAATGCA AGTCTTGTCA ATTTACAAGA GAGAAAACAG 
CTGACCCTCT CACCAGTGAT TGAGAGGATG TGCTTTACCC ACTCTGAGAA AATACCACAG 
TCTCCCAGTT TTAAGGAAGT GGATATTTTT ATTGGAAACA GTGACTCTAA AAAGGAAATT 
GATCTTAGTA AGTATTATCC TGATGATGCA GCCGAGGAAA TGAAGGCCCT TCTGGGAATA 
TTTAATGGCA TTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mouse HFM1/Mer3 is required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
PLoS genetics9(3)e1003383(2013 Mar)
Guiraldelli MF,Eyster C,Wilkerson JL,Dresser ME,Pezza RJ

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Hfm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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