hfm1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hfm1
Entrez Gene ID	779837
Full Name	HFM1, ATP-dependent DNA helicase homolog
Synonyms	mer3,sec63d1
General protein information	Preferred Names HFM1, ATP-dependent DNA helicase homolog Names probable ATP-dependent DNA helicase HFM1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031900197.1	XP_031756057.1	probable ATP-dependent DNA helicase HFM1 isoform X3
XM_018093091.1	XP_017948580.1	probable ATP-dependent DNA helicase HFM1 isoform X1
NM_001078915.2	NP_001072383.3	probable ATP-dependent DNA helicase HFM1
XM_031900196.1	XP_031756056.1	probable ATP-dependent DNA helicase HFM1 isoform X1
XM_018093091.2	XP_017948580.2	probable ATP-dependent DNA helicase HFM1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HFM1	NP_001017975.3
Danio rerio (zebrafish)	hfm1	XP_001919555.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009060	XP_552976.3
Arabidopsis thaliana (thale cress)	RCK	NP_189410.2
Xenopus tropicalis (tropical clawed frog)	hfm1	NP_001072383.3
Pan troglodytes (chimpanzee)	HFM1	XP_524618.3
Oryza sativa (rice)	Os02g0617500	NP_001047442.1
Rattus norvegicus (Norway rat)	Hfm1	NP_001178030.1
	LOC704516	XP_002808271.1
Canis lupus familiaris (dog)	HFM1	XP_005621996.1
Bos taurus (cattle)	HFM1	NP_001192505.1
Mus musculus (house mouse)	Hfm1	NP_808541.2
Gallus gallus (chicken)	HFM1	XP_422349.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hfm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hfm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43830	XM_018093091.1 Latest version!	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa56156	XM_031900196.1 Latest version!	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56157	XM_018093091.2 Latest version!	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56158	XM_031900197.1 Latest version!	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa01166	NM_001078915.2 Latest version!	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hfm1 ( XM_018093091.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948580.1 Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa43830
Clone ID Related Accession (Same CDS sequence)	XM_018093091.1
Accession Version	XM_018093091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent DNA helicase HFM1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC CATTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGAGGT AATGGCACTA 
AAGAAGTTTC AGCAACCATT TAAAGAAAGT AATGAAACAG AAAGTAAAGC TTCTGTTTCA 
TGTGCCTCCC AGTGTGGAAA CAAAACAAAA AAAACAAGCT TGGAGGGCAT GACATTACCA 
TTTTCATGGA TTACAAATGA TGTTTCCCAA TGTATAGATT TAACGCAGGA TGAACATATC 
TGTTCATTAA GCCCAGTTAC AGATACTGAG GAAAATATGA GTGCATTCAA AAAAAGCTTG 
TTTGGGAAAT ATGATTGTTC TTTAAACAGT GAAGACCAAT GTCTTATAGT GGAAAATGGG 
AGTTCTAATT TTGTAACCTC AAAAGAAGCA CAAAAAGAAG CAATGAAAGC AGATTCTGAC 
ATATGCCCAC CTCAGCAAAT GGCTTTCAAT TTATTTAATG AAACTGTAGT TCAAAAGGAC 
ATGCTGAATA AGGAGAAATT TTCTCACTCT GCTTCTTCAA ATATCTCTAA CAAAGATGAT 
GCACAATATG GAATTGTTAC ACCTGTCACA GAAATCCCTA AGCAATTCCG GACTGTCTTC 
AAAGAATTTC CATACTTTAA TTATATACAG TCGAAAGCAT TGGTGCATCT TCTTTATTCA 
GACAGAAACT TTGTGCTTTG TGCTCCAACT GGATCTGGAA AGACAGTTAT ATTTGAACTT 
GCAATTATTC GATTGCTAAT GCAAGTGCCA ATGCCTTGGA CAAATGTTAA AATAGTATAC 
ATGGCACCAA TCAAAGCTTT ATGTGGTCAG AGATATGATG ACTGGAAAGC AAAATTTGGA 
CCAGTTGGTT TGAATTGCAA AGAGTTAACT GGTGATACTG AAATGGATGA TCTTTTTGAA 
ATTCAGCATG CACACATCAT CATGACAACT CCAGAAAAAT GGGATAGTAT GACGAGAAAA 
TGGAAAGATA ATACTTTGGT TCAGCTTGTA CGTTTGTTTC TGATTGATGA GGTGCATATT 
TTGAAGGAAG AAAATCGAGG TGCAACACTT GAGGTTGTCG TCAGCAGAAT GAAAACAATC 
TATTCTCTTT CTCATTTGTC AGAAGATAGA AAGGCATTCA TACCGATGAG ATTTGTGGCA 
GTTTCTGCAA CCATTCCTAA TGTGGAAGAT ATAGCAGATT GGTTGTCGGA TGAAAACAGT 
CCTGGTGTAT GCATGAAAAT GGATGAATCA AGCAGACCAG TGAAACTGCG CAAAGTGGTG 
TTAGGCTTTC CTTGCAGCAC CAAGCAGTCA GAGTTCAAGT TTGATCTAAC ACTAAATTAC 
AAAATAGCTA ATATAATACA AACCTATTCA GATGGGAGAC CAACCCTTGT GTTTTGTTCT 
ACAAGGAAAG GAGTACAACA AGCAGCGTCT ATTCTAACCA AAGATGCAAA GTTTGTAATG 
AGCATTGAAC ACAAGCAGAG GCTTCAGAAA TGTGCTAATT CAATTAAAGA TTCTAAGCTA 
AGAGATGTTT TACAATATGG TGTTGGTTAC CACCATGCTG GTGTTGATAT ATCTGACAGG 
AAAGTCATTG AAAACTCTTT TTTAATTGGA GATCTTCCAG TACTTTTCAC CACAAGTACC 
TTAGCTATGG GAGTAAACCT TCCAGCTCAT TTAGTTATTG TAAAATCAAC AATGCATTAT 
GTCTCTGGAA TGTTTCAAGA ATATAGTGAG ACAGATATTC TTCAGATGAT TGGAAGAGCT 
GGAAGACCTC AGTTTGATTC TACAGCCACA GCCGTCATTA TGACCAGATT AAGCACCAAA 
GAAAAATATG TACACATGTT AGATGGTGCA GATACAATAG AAAGCAGCTT GCACAAGCAT 
CTTGTTGAGC ATCTGAATGC AGAAATAGCA CTGCACACCA TTACAGATGT TAAAGTTGCT 
TTGGAGTGGA TAAGATCGAC TTTCTTGTAT ATCAGAGCGC TGAAGAACCC AGCTTATTAT 
GGCTTTTCTG AAGGATTAGA CAAGATTGGA ATTGAAGCAA AATTGCAAGA ATTGTGCTTA 
AAAAATCTAA ATGATTTGTC TTCTCTGGGA TTAATTAAAA TGGATGAAGA AATTAATTTT 
AAACCCACAG AAACTGGAAA ACTGATGGCT CTGTATTACA TTGCATTTAA CACAGCAAAA 
TTGTTTCATA CAATTAGAGG AACAGAAACA CTAGCTGAAT TGGTGTCACT GATATCATCA 
TGTTCAGAAT TCTCAGATGT GCAGCTAAGA GCAAATGAAA GAAGAGTTTT GAACACTTTA 
AATAAAGACA AGAACAGAGT AACTATTAGA TATCCAATGG AAGGGAAGAT TAAAACCAAA 
GAAATGAAAG TTAACTGCCT TATTCAAGCT CATCTTGGGT GCATTCTTGT TCAAGACTTC 
TCTCTGACAC AAGATATTTC AAAGATTTTC AGGCATGGCA CACGATTGGC TAAATGTTTA 
TCAGAGTTTC TTGCATTGCA AGAAAACAAG TTCTCTGCAT TTCTAAATGC ACTAATTTTG 
ACCAAGTGTT TCAAAAGCAA ACTTTGGGAA AATTCATCAC ACATTTCTAA GCAACTGGAA 
AAAATTGGTG TCACTCTGGC AAATGCAATG GTAAATGCAG GACTTACAAC ATTTAAAAAA 
ATAGAAGATA CAAATGCCAG AGAACTTGAA TTGATTGTCA ATAGGCACCC ACCCTTTGGA 
AATCAAATAA AAGAATCTGT TGCACACCTT CCAAAGTATG AAATAAAGTT CCAGCAGCTA 
GCAAAGTACA GAGCTACAAC TGCAGAAGTT GTTTTGACTG TTCTCTTGAC AAATTTCAAG 
CAGCTGCAGA AGAAAAGAAC AGCTCCAGAT TCACATTTTG TAATGCTTGT TATTGGAGAT 
GATGATAATC AAGCTATCTT CAAACAGAAA ATTATGGACT CCTCTCTTTT TAAAACTGGA 
AGTTGGACAA GAAAAATTGA AATCAAAAGA GCATCTAAAT CAACTAATGT TTGCTTGAAT 
CTCATCAGCT CAGAATATGT TGGCCTTGAC ATCCAACAAA GTTTCAACAT TTGCTATCTG 
GGATCAGACA GTTTTAATGC AGATCTGGTG ACTCAGCATA AATCTGAAAA TATATTTTCA 
CAAGAAATAT ATAAAACTTC CACAAAAACT AAAGATATTG CTGATACTAA TTGGTCTATG 
AGACAGACAG CCATAAAATG TGCTACTAGA GAATGTAATC ATAATTGCAA GAATAAAGAT 
GCCTGTGGAC ATGAATGCTG TAAAGTTGGA GTGTCTGAGA AGGCTGAGAT GAAGACAAAC 
TTCTATTCTT ACCTAAATGA TCTAAGGACT CGAAATTCAG TTTCATCAGC TACACCAGTG 
AAAAGACTAA AGATGCAGAT GGAAGCTGAA ACTAGGAATG TGAATTTACA GAACTTTGTC 
TTCACGCCAA AACCTTCAAT AAGGTCACCA TGTCTGAAAA TTCCTGAAAA CATTTTACTT 
CAAGCAGAGT CTTCTGTTTT GACAAACAAA TATAAAGCTT CACATCAAGA AGAAACATGT 
TGTAAATATG AAATTAAAGG TTGCGGAGAA AAGCAAACAA AAGGGATGCC AAAAAAAACA 
TGTGAAAGGA GAAATCTCAC TGATTCAGAC AAATGCAACT CATCGTCATC AGAGAACGCA 
GCATACGTGA CATTTGACTT GGGCAATGAC ATATGGGATG ATTTTGATGA TGATAATCTT 
TTAGATGCTA GTAATATTTC AAGTTTAACT GACCAACACA CTTCAGAATT TACAGACGTC 
TCGTTCAGTA AGAACCAACC TGTATCAAAG TCCTCATTGT CAGCAGGACA CCATGAAGAA 
GCAAGATACA ATGCAATGAG TATAAACAAG GATTCCTATA GATATTTAAA TGCTAAGTCA 
GAAGTTTTTT CTCAAGAAAA CACCCAGTGC ATTGATGTTG TACCATCAAA TCAAAAATTA 
CATGGAAAGA TGTTTTCTGA GAATACTATA AAACATATGT TTACCCTTCA AGAAGAAACC 
AATTACCCTG TTACCTCCAA AATGGATTCT CCACACAATG GTTGCTGGTC AAGTAACATT 
TTTTTCAAGA CTCCAGAAAA TAAAACACAA GAGAGAGGAT GTTATCATCA GGAAGATGAA 
ACAAATGCTT TCATTGGAAT ATTTAATGGA ATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948580.1
CDS	123..4421
Translation	METPFNSLHSLNGLFSEVPDVSVLQKEELKMWLKAPAPGIAEIPLTQDLQNELSTEVMALKKFQQPFKESNETESKASVSCASQCGNKTKKTSLEGMTLPFSWITNDVSQCIDLTQDEHICSLSPVTDTEENMSAFKKSLFGKYDCSLNSEDQCLIVENGSSNFVTSKEAQKEAMKADSDICPPQQMAFNLFNETVVQKDMLNKEKFSHSASSNISNKDDAQYGIVTPVTEIPKQFRTVFKEFPYFNYIQSKALVHLLYSDRNFVLCAPTGSGKTVIFELAIIRLLMQVPMPWTNVKIVYMAPIKALCGQRYDDWKAKFGPVGLNCKELTGDTEMDDLFEIQHAHIIMTTPEKWDSMTRKWKDNTLVQLVRLFLIDEVHILKEENRGATLEVVVSRMKTIYSLSHLSEDRKAFIPMRFVAVSATIPNVEDIADWLSDENSPGVCMKMDESSRPVKLRKVVLGFPCSTKQSEFKFDLTLNYKIANIIQTYSDGRPTLVFCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLDGADTIESSLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEINFKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELVSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQENKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAPDSHFVMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLGSDSFNADLVTQHKSENIFSQEIYKTSTKTKDIADTNWSMRQTAIKCATRECNHNCKNKDACGHECCKVGVSEKAEMKTNFYSYLNDLRTRNSVSSATPVKRLKMQMEAETRNVNLQNFVFTPKPSIRSPCLKIPENILLQAESSVLTNKYKASHQEETCCKYEIKGCGEKQTKGMPKKTCERRNLTDSDKCNSSSSENAAYVTFDLGNDIWDDFDDDNLLDASNISSLTDQHTSEFTDVSFSKNQPVSKSSLSAGHHEEARYNAMSINKDSYRYLNAKSEVFSQENTQCIDVVPSNQKLHGKMFSENTIKHMFTLQEETNYPVTSKMDSPHNGCWSSNIFFKTPENKTQERGCYHQEDETNAFIGIFNGIF

Target ORF information:

RefSeq Version	XM_018093091.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093091.1

ORF Insert Sequence:

ATGGAGACAC CATTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGAGGT AATGGCACTA 
AAGAAGTTTC AGCAACCATT TAAAGAAAGT AATGAAACAG AAAGTAAAGC TTCTGTTTCA 
TGTGCCTCCC AGTGTGGAAA CAAAACAAAA AAAACAAGCT TGGAGGGCAT GACATTACCA 
TTTTCATGGA TTACAAATGA TGTTTCCCAA TGTATAGATT TAACGCAGGA TGAACATATC 
TGTTCATTAA GCCCAGTTAC AGATACTGAG GAAAATATGA GTGCATTCAA AAAAAGCTTG 
TTTGGGAAAT ATGATTGTTC TTTAAACAGT GAAGACCAAT GTCTTATAGT GGAAAATGGG 
AGTTCTAATT TTGTAACCTC AAAAGAAGCA CAAAAAGAAG CAATGAAAGC AGATTCTGAC 
ATATGCCCAC CTCAGCAAAT GGCTTTCAAT TTATTTAATG AAACTGTAGT TCAAAAGGAC 
ATGCTGAATA AGGAGAAATT TTCTCACTCT GCTTCTTCAA ATATCTCTAA CAAAGATGAT 
GCACAATATG GAATTGTTAC ACCTGTCACA GAAATCCCTA AGCAATTCCG GACTGTCTTC 
AAAGAATTTC CATACTTTAA TTATATACAG TCGAAAGCAT TGGTGCATCT TCTTTATTCA 
GACAGAAACT TTGTGCTTTG TGCTCCAACT GGATCTGGAA AGACAGTTAT ATTTGAACTT 
GCAATTATTC GATTGCTAAT GCAAGTGCCA ATGCCTTGGA CAAATGTTAA AATAGTATAC 
ATGGCACCAA TCAAAGCTTT ATGTGGTCAG AGATATGATG ACTGGAAAGC AAAATTTGGA 
CCAGTTGGTT TGAATTGCAA AGAGTTAACT GGTGATACTG AAATGGATGA TCTTTTTGAA 
ATTCAGCATG CACACATCAT CATGACAACT CCAGAAAAAT GGGATAGTAT GACGAGAAAA 
TGGAAAGATA ATACTTTGGT TCAGCTTGTA CGTTTGTTTC TGATTGATGA GGTGCATATT 
TTGAAGGAAG AAAATCGAGG TGCAACACTT GAGGTTGTCG TCAGCAGAAT GAAAACAATC 
TATTCTCTTT CTCATTTGTC AGAAGATAGA AAGGCATTCA TACCGATGAG ATTTGTGGCA 
GTTTCTGCAA CCATTCCTAA TGTGGAAGAT ATAGCAGATT GGTTGTCGGA TGAAAACAGT 
CCTGGTGTAT GCATGAAAAT GGATGAATCA AGCAGACCAG TGAAACTGCG CAAAGTGGTG 
TTAGGCTTTC CTTGCAGCAC CAAGCAGTCA GAGTTCAAGT TTGATCTAAC ACTAAATTAC 
AAAATAGCTA ATATAATACA AACCTATTCA GATGGGAGAC CAACCCTTGT GTTTTGTTCT 
ACAAGGAAAG GAGTACAACA AGCAGCGTCT ATTCTAACCA AAGATGCAAA GTTTGTAATG 
AGCATTGAAC ACAAGCAGAG GCTTCAGAAA TGTGCTAATT CAATTAAAGA TTCTAAGCTA 
AGAGATGTTT TACAATATGG TGTTGGTTAC CACCATGCTG GTGTTGATAT ATCTGACAGG 
AAAGTCATTG AAAACTCTTT TTTAATTGGA GATCTTCCAG TACTTTTCAC CACAAGTACC 
TTAGCTATGG GAGTAAACCT TCCAGCTCAT TTAGTTATTG TAAAATCAAC AATGCATTAT 
GTCTCTGGAA TGTTTCAAGA ATATAGTGAG ACAGATATTC TTCAGATGAT TGGAAGAGCT 
GGAAGACCTC AGTTTGATTC TACAGCCACA GCCGTCATTA TGACCAGATT AAGCACCAAA 
GAAAAATATG TACACATGTT AGATGGTGCA GATACAATAG AAAGCAGCTT GCACAAGCAT 
CTTGTTGAGC ATCTGAATGC AGAAATAGCA CTGCACACCA TTACAGATGT TAAAGTTGCT 
TTGGAGTGGA TAAGATCGAC TTTCTTGTAT ATCAGAGCGC TGAAGAACCC AGCTTATTAT 
GGCTTTTCTG AAGGATTAGA CAAGATTGGA ATTGAAGCAA AATTGCAAGA ATTGTGCTTA 
AAAAATCTAA ATGATTTGTC TTCTCTGGGA TTAATTAAAA TGGATGAAGA AATTAATTTT 
AAACCCACAG AAACTGGAAA ACTGATGGCT CTGTATTACA TTGCATTTAA CACAGCAAAA 
TTGTTTCATA CAATTAGAGG AACAGAAACA CTAGCTGAAT TGGTGTCACT GATATCATCA 
TGTTCAGAAT TCTCAGATGT GCAGCTAAGA GCAAATGAAA GAAGAGTTTT GAACACTTTA 
AATAAAGACA AGAACAGAGT AACTATTAGA TATCCAATGG AAGGGAAGAT TAAAACCAAA 
GAAATGAAAG TTAACTGCCT TATTCAAGCT CATCTTGGGT GCATTCTTGT TCAAGACTTC 
TCTCTGACAC AAGATATTTC AAAGATTTTC AGGCATGGCA CACGATTGGC TAAATGTTTA 
TCAGAGTTTC TTGCATTGCA AGAAAACAAG TTCTCTGCAT TTCTAAATGC ACTAATTTTG 
ACCAAGTGTT TCAAAAGCAA ACTTTGGGAA AATTCATCAC ACATTTCTAA GCAACTGGAA 
AAAATTGGTG TCACTCTGGC AAATGCAATG GTAAATGCAG GACTTACAAC ATTTAAAAAA 
ATAGAAGATA CAAATGCCAG AGAACTTGAA TTGATTGTCA ATAGGCACCC ACCCTTTGGA 
AATCAAATAA AAGAATCTGT TGCACACCTT CCAAAGTATG AAATAAAGTT CCAGCAGCTA 
GCAAAGTACA GAGCTACAAC TGCAGAAGTT GTTTTGACTG TTCTCTTGAC AAATTTCAAG 
CAGCTGCAGA AGAAAAGAAC AGCTCCAGAT TCACATTTTG TAATGCTTGT TATTGGAGAT 
GATGATAATC AAGCTATCTT CAAACAGAAA ATTATGGACT CCTCTCTTTT TAAAACTGGA 
AGTTGGACAA GAAAAATTGA AATCAAAAGA GCATCTAAAT CAACTAATGT TTGCTTGAAT 
CTCATCAGCT CAGAATATGT TGGCCTTGAC ATCCAACAAA GTTTCAACAT TTGCTATCTG 
GGATCAGACA GTTTTAATGC AGATCTGGTG ACTCAGCATA AATCTGAAAA TATATTTTCA 
CAAGAAATAT ATAAAACTTC CACAAAAACT AAAGATATTG CTGATACTAA TTGGTCTATG 
AGACAGACAG CCATAAAATG TGCTACTAGA GAATGTAATC ATAATTGCAA GAATAAAGAT 
GCCTGTGGAC ATGAATGCTG TAAAGTTGGA GTGTCTGAGA AGGCTGAGAT GAAGACAAAC 
TTCTATTCTT ACCTAAATGA TCTAAGGACT CGAAATTCAG TTTCATCAGC TACACCAGTG 
AAAAGACTAA AGATGCAGAT GGAAGCTGAA ACTAGGAATG TGAATTTACA GAACTTTGTC 
TTCACGCCAA AACCTTCAAT AAGGTCACCA TGTCTGAAAA TTCCTGAAAA CATTTTACTT 
CAAGCAGAGT CTTCTGTTTT GACAAACAAA TATAAAGCTT CACATCAAGA AGAAACATGT 
TGTAAATATG AAATTAAAGG TTGCGGAGAA AAGCAAACAA AAGGGATGCC AAAAAAAACA 
TGTGAAAGGA GAAATCTCAC TGATTCAGAC AAATGCAACT CATCGTCATC AGAGAACGCA 
GCATACGTGA CATTTGACTT GGGCAATGAC ATATGGGATG ATTTTGATGA TGATAATCTT 
TTAGATGCTA GTAATATTTC AAGTTTAACT GACCAACACA CTTCAGAATT TACAGACGTC 
TCGTTCAGTA AGAACCAACC TGTATCAAAG TCCTCATTGT CAGCAGGACA CCATGAAGAA 
GCAAGATACA ATGCAATGAG TATAAACAAG GATTCCTATA GATATTTAAA TGCTAAGTCA 
GAAGTTTTTT CTCAAGAAAA CACCCAGTGC ATTGATGTTG TACCATCAAA TCAAAAATTA 
CATGGAAAGA TGTTTTCTGA GAATACTATA AAACATATGT TTACCCTTCA AGAAGAAACC 
AATTACCCTG TTACCTCCAA AATGGATTCT CCACACAATG GTTGCTGGTC AAGTAACATT 
TTTTTCAAGA CTCCAGAAAA TAAAACACAA GAGAGAGGAT GTTATCATCA GGAAGATGAA 
ACAAATGCTT TCATTGGAAT ATTTAATGGA ATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hfm1 ( XM_031900196.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756056.1 Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa56156
Clone ID Related Accession (Same CDS sequence)	XM_031900196.1
Accession Version	XM_031900196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent DNA helicase HFM1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC CAGTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGCAGA GGTAATGGCA 
CTAAAGAAGT TTCAGCAACC ATTTAAAGAA AGTAATGAAA CAGAAAGTAA AGCTTCTGTT 
TCATGTGCCT CCCAGTGTGG AAACAAAACA AAAAAAACAA GCTTGGAGGG CATGACATTA 
CCATTTTCAT GGATTACAAA TGATGTTTCC CAATGTATAG ATTTAACGCA GGATGAACAT 
ATCTGTTCAT TAAGCCCAGT TACAGATACT GAGGAAAATA TGAGTGCATT CAAAAAAAGC 
TTGTTTGGGA AATATGATTG TTCTTTAAAC AGTGAAGACC AATGTCTTAT AGTGGAAAAT 
GGGAGTTCTA ATTTTGTAAC CTCAAAAGAA GCACAAAAAG AAGCAATGAA AGCAGATTTT 
GACATATGCC CACCTCAGCA AATGGCTTTC GATTTATTTA ATGAAACTGT AGTTCAAAAG 
GACATGCTGA ATAAGGAGAA ATTTTCTCAC TCTGCTTCTT CAAATATCTC TAACAAAGAT 
GATGCACAAT ATGGAATTGT TACACCTGTC ACAGAAATCC CTAAGCAATT CCGGACTGTC 
TTCAAAGAAT TTCCATACTT TAATTATATA CAGTCGAAAG CATTGGAGCA TCTTCTTTAT 
TCAGACAGAA ACTTTGTGCT TTGTGCTCCA ACTGGATCTG GAAAGACAGT TATATTTGAA 
CTTGCAATTA TTCGATTGCT AATGCAAGTG CCAATGCCTT GGACAAATGT TAAAATAGTA 
TACATGGCAC CAATCAAAGC TTTATGTGGT CAGAGATATG ATGACTGGAA AGCAAAATTT 
GGACCAGTTG GTTTGAATTG CAAAGAGTTA ACTGGTGATA CTGAAATGGA TGATCTTTTT 
GAAATTCAGC ATGCACACAT CATCATGACA ACTCCAGAAA AATGGGATAG TATGACGAGA 
AAATGGAAAG ATAATACTTT GGTTCAGCTT GTACGTTTGT TTCTGATTGA TGAGGTGCAT 
ATTTTGAAGG AAGAAAATCG AGGTGCAACA CTTGAGGTTG TCGTCAGCAG AATGAAAACA 
ATCTATTCTC TTTCTCATTT GTCAGAAGAT AGAAAGGCAT TCATACCGAT GAGATTTGTG 
GCAGTTTCTG CAACCATTCC TAATGTGGAA GATATAGCGG ATTGGTTGTC GGATGAAAAC 
AGTCCTGGTG TATGCATGAA AATGGATGAA TCAAGCAGAC CAGTGAAACT GCGCAAAGTG 
GTGTTAGGCT TTCCTTGCAG CACCAAGCAG TCAGAGTTCA AGTTTGATCT AACACTAAAT 
TACAAAATAG CTAATATAAT ACAAACCTAT TCAGATGGGA GACCAACCCT TGTGTTTTGT 
TCTACAAGGA AAGGAGTACA ACAAGCAGCG TCTATTCTAA CCAAAGATGC AAAGTTTGTA 
ATGAGCATTG AACACAAGCA GAGGCTTCAG AAATGTGCTA ATTCAATTAA AGATTCTAAG 
CTAAGAGATG TTTTACAATA TGGTGTTGGT TACCACCATG CTGGTGTTGA TATATCTGAC 
AGGAAAGTCA TTGAAAACTC TTTTTTAATT GGAGATCTTC CAGTACTTTT CACCACAAGT 
ACCTTAGCTA TGGGAGTAAA CCTTCCAGCT CATTTAGTTA TTGTAAAATC AACAATGCAT 
TATGTCTCTG GAATGTTTCA AGAATATAGT GAGACAGATA TTCTTCAGAT GATTGGAAGA 
GCTGGAAGAC CTCAGTTTGA TTCTACAGCC ACAGCCGTCA TTATGACCAG ATTAAGCACC 
AAAGAAAAAT ATGTACACAT GTTAGATGGT GCAGATACAA TAGAAAGCAG CTTGCACAAG 
CATCTTGTTG AGCATCTGAA TGCAGAAATA GCACTGCACA CCATTACAGA TGTTAAAGTT 
GCTTTGGAGT GGATAAGATC GACTTTCTTG TATATCAGAG CGCTGAAGAA CCCAGCTTAT 
TATGGCTTTT CTGAAGGATT AGACAAGATT GGAATTGAAG CAAAATTGCA AGAATTGTGC 
TTAAAAAATC TAAATGATTT GTCTTCTCTG GGATTAATTA AAATGGATGA AGAAATTAAT 
TTTAAACCCA CAGAAACTGG AAAACTGATG GCTCTGTATT ACATTGCATT TAACACAGCA 
AAATTGTTTC ATACAATTAG AGGAACAGAA ACACTAGCTG AATTGGTGTC ACTGATATCA 
TCATGTTCAG AATTCTCAGA TGTGCAGCTA AGAGCAAATG AAAGAAGAGT TTTGAACACT 
TTAAATAAAG ACAAGAACAG AGTAACTATC AGATATCCAA TGGAAGGGAA GATTAAAACC 
AAAGAAATGA AAGTTAACTG CCTTATTCAA GCTCATCTTG GGTGCATTCT TGTTCAAGAC 
TTCTCTCTGA CACAAGATAT TTCAAAGATT TTCAGGCATG GCACACGATT GGCTAAATGT 
TTATCAGAGT TTCTTGCATT GCAAGAAAAC AAGTTCTCTG CATTTCTAAA TGCACTAATT 
TTGACCAAGT GTTTCAAAAG CAAACTTTGG GAAAATTCAT CACACATTTC TAAGCAACTG 
GAAAAAATTG GTGTCACTCT GGCAAATGCA ATGGTAAATG CAGGACTTAC AACATTTAAA 
AAAATAGAAG ATACAAATGC CAGAGAACTT GAATTGATTG TCAATAGGCA CCCACCCTTT 
GGAAATCAAA TAAAAGAATC TGTTGCACAC CTTCCAAAGT ATGAAATAAA GTTCCAGCAG 
CTAGCAAAGT ACAGAGCTAC AACTGCAGAA GTTGTTTTGA CTGTTCTCTT GACAAATTTC 
AAGCAGCTGC AGAAGAAAAG AACAGCTCCA GATTCACATT TTGTAATGCT TGTTATTGGA 
GATGATGATA ATCAAGCTAT CTTCAAACAG AAAATTATGG ACTCCTCTCT TTTTAAAACT 
GGAAGTTGGA CAAGAAAAAT TGAAATCAAA AGAGCATCTA AATCAACTAA TGTTTGCTTG 
AATCTCATCA GCTCAGAATA TGTTGGCCTT GACATCCAAC AAAGTTTCAA CATTTGCTAT 
CTGGGATCAG ACAGTTTTAA TGCAGATCTG GTGACTCAGC ATAAATCTGA AAATATATTT 
TCACAAGAAA TATATAAAAC TTCCACAAAA ACTAAAGATA TTGCTGATAC TAATTGGTCT 
ATGAGACAGA CAGCCATAAA ATGTGCTACT AGAGAATGTA ATCATAATTG CAAGAATAAA 
GATGCCTGTG GACATGAATG CTGTAAAGTT GGAGTGTCTG AGAAGGCTGA GATGAAGACA 
AACTTCTATT CTTACCTAAA TGATCTAAGG ACTCGAAATT CAGTTTCATC AGCTACACCA 
GTGAAAAGAC TAAAGATGCA GATGGAAGCT GAAACTAGGA ATGTGAATTT ACAGAACTTT 
GTCTTCACGC CAAAACCTTC AATAAGGTCA CCATGTCTGA AAATTCCTGA AAACATTTTA 
CTTCAAGCAG AGTCTTCTGT TTTGACAAAC AAATATAAAG CTTCACATCA AGAAGAAACA 
TGTTGTAAAT ATGAAATTAA AGGTTGCGGA GAAAAGCAAA CAAAAGGGAT GCCAAAAAAA 
ACATGTGAAA GGAGAAATCT CACTGATTCA GACAAATGCA ACTCATCGTC ATCAGAGAAC 
GCAGCATACG TGACATTTGA CTTGGGCAAT GACATATGGG ATGATTTTGA TGATGATAAT 
CTTTTAGATG CTAGTAATAT TTCAAGTTTA ACTGACCAAC ACACTTCAGA ATTTACAGAC 
GTCTCGTTCA GTAAGAACCA ACCTGTATCA AAGTCCTCAT TGTCAGCAGG ACACCATGAA 
GAAGCAAGAT ACAATGCAAT GAGTATAAAC AAGGATTCCT ATAGATATTT AAATGCTAAG 
TCAGAAGTTT TTTCTCAAGA AAACACCCAG TGCATTGATG TTGTACCATC AAATCAAAAA 
TTACATGGAA AGATGTTTTC TGAGAATACT ATAAAACATA TGTTTACCCT TCAAGAAGAA 
ACCAATTACC CTGTTACCTC CAAAATGGAT TCTCCACACA ATGGTTGCTG GTCAAGTAAC 
ATTTTTTTCA AGACTCCAGA AAATAAAACA CAAGAGAGAG GATGTTATCA TCAGGAAGAT 
GAAACAAATG CTTTCATTGG AATATTTAAT GGAATATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756056.1
CDS	99..4400
Translation	METPVNSLHSLNGLFSEVPDVSVLQKEELKMWLKAPAPGIAEIPLTQDLQNELSTAEVMALKKFQQPFKESNETESKASVSCASQCGNKTKKTSLEGMTLPFSWITNDVSQCIDLTQDEHICSLSPVTDTEENMSAFKKSLFGKYDCSLNSEDQCLIVENGSSNFVTSKEAQKEAMKADFDICPPQQMAFDLFNETVVQKDMLNKEKFSHSASSNISNKDDAQYGIVTPVTEIPKQFRTVFKEFPYFNYIQSKALEHLLYSDRNFVLCAPTGSGKTVIFELAIIRLLMQVPMPWTNVKIVYMAPIKALCGQRYDDWKAKFGPVGLNCKELTGDTEMDDLFEIQHAHIIMTTPEKWDSMTRKWKDNTLVQLVRLFLIDEVHILKEENRGATLEVVVSRMKTIYSLSHLSEDRKAFIPMRFVAVSATIPNVEDIADWLSDENSPGVCMKMDESSRPVKLRKVVLGFPCSTKQSEFKFDLTLNYKIANIIQTYSDGRPTLVFCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLDGADTIESSLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEINFKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELVSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQENKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAPDSHFVMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLGSDSFNADLVTQHKSENIFSQEIYKTSTKTKDIADTNWSMRQTAIKCATRECNHNCKNKDACGHECCKVGVSEKAEMKTNFYSYLNDLRTRNSVSSATPVKRLKMQMEAETRNVNLQNFVFTPKPSIRSPCLKIPENILLQAESSVLTNKYKASHQEETCCKYEIKGCGEKQTKGMPKKTCERRNLTDSDKCNSSSSENAAYVTFDLGNDIWDDFDDDNLLDASNISSLTDQHTSEFTDVSFSKNQPVSKSSLSAGHHEEARYNAMSINKDSYRYLNAKSEVFSQENTQCIDVVPSNQKLHGKMFSENTIKHMFTLQEETNYPVTSKMDSPHNGCWSSNIFFKTPENKTQERGCYHQEDETNAFIGIFNGIF

Target ORF information:

RefSeq Version	XM_031900196.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900196.1

ORF Insert Sequence:

ATGGAGACAC CAGTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGCAGA GGTAATGGCA 
CTAAAGAAGT TTCAGCAACC ATTTAAAGAA AGTAATGAAA CAGAAAGTAA AGCTTCTGTT 
TCATGTGCCT CCCAGTGTGG AAACAAAACA AAAAAAACAA GCTTGGAGGG CATGACATTA 
CCATTTTCAT GGATTACAAA TGATGTTTCC CAATGTATAG ATTTAACGCA GGATGAACAT 
ATCTGTTCAT TAAGCCCAGT TACAGATACT GAGGAAAATA TGAGTGCATT CAAAAAAAGC 
TTGTTTGGGA AATATGATTG TTCTTTAAAC AGTGAAGACC AATGTCTTAT AGTGGAAAAT 
GGGAGTTCTA ATTTTGTAAC CTCAAAAGAA GCACAAAAAG AAGCAATGAA AGCAGATTTT 
GACATATGCC CACCTCAGCA AATGGCTTTC GATTTATTTA ATGAAACTGT AGTTCAAAAG 
GACATGCTGA ATAAGGAGAA ATTTTCTCAC TCTGCTTCTT CAAATATCTC TAACAAAGAT 
GATGCACAAT ATGGAATTGT TACACCTGTC ACAGAAATCC CTAAGCAATT CCGGACTGTC 
TTCAAAGAAT TTCCATACTT TAATTATATA CAGTCGAAAG CATTGGAGCA TCTTCTTTAT 
TCAGACAGAA ACTTTGTGCT TTGTGCTCCA ACTGGATCTG GAAAGACAGT TATATTTGAA 
CTTGCAATTA TTCGATTGCT AATGCAAGTG CCAATGCCTT GGACAAATGT TAAAATAGTA 
TACATGGCAC CAATCAAAGC TTTATGTGGT CAGAGATATG ATGACTGGAA AGCAAAATTT 
GGACCAGTTG GTTTGAATTG CAAAGAGTTA ACTGGTGATA CTGAAATGGA TGATCTTTTT 
GAAATTCAGC ATGCACACAT CATCATGACA ACTCCAGAAA AATGGGATAG TATGACGAGA 
AAATGGAAAG ATAATACTTT GGTTCAGCTT GTACGTTTGT TTCTGATTGA TGAGGTGCAT 
ATTTTGAAGG AAGAAAATCG AGGTGCAACA CTTGAGGTTG TCGTCAGCAG AATGAAAACA 
ATCTATTCTC TTTCTCATTT GTCAGAAGAT AGAAAGGCAT TCATACCGAT GAGATTTGTG 
GCAGTTTCTG CAACCATTCC TAATGTGGAA GATATAGCGG ATTGGTTGTC GGATGAAAAC 
AGTCCTGGTG TATGCATGAA AATGGATGAA TCAAGCAGAC CAGTGAAACT GCGCAAAGTG 
GTGTTAGGCT TTCCTTGCAG CACCAAGCAG TCAGAGTTCA AGTTTGATCT AACACTAAAT 
TACAAAATAG CTAATATAAT ACAAACCTAT TCAGATGGGA GACCAACCCT TGTGTTTTGT 
TCTACAAGGA AAGGAGTACA ACAAGCAGCG TCTATTCTAA CCAAAGATGC AAAGTTTGTA 
ATGAGCATTG AACACAAGCA GAGGCTTCAG AAATGTGCTA ATTCAATTAA AGATTCTAAG 
CTAAGAGATG TTTTACAATA TGGTGTTGGT TACCACCATG CTGGTGTTGA TATATCTGAC 
AGGAAAGTCA TTGAAAACTC TTTTTTAATT GGAGATCTTC CAGTACTTTT CACCACAAGT 
ACCTTAGCTA TGGGAGTAAA CCTTCCAGCT CATTTAGTTA TTGTAAAATC AACAATGCAT 
TATGTCTCTG GAATGTTTCA AGAATATAGT GAGACAGATA TTCTTCAGAT GATTGGAAGA 
GCTGGAAGAC CTCAGTTTGA TTCTACAGCC ACAGCCGTCA TTATGACCAG ATTAAGCACC 
AAAGAAAAAT ATGTACACAT GTTAGATGGT GCAGATACAA TAGAAAGCAG CTTGCACAAG 
CATCTTGTTG AGCATCTGAA TGCAGAAATA GCACTGCACA CCATTACAGA TGTTAAAGTT 
GCTTTGGAGT GGATAAGATC GACTTTCTTG TATATCAGAG CGCTGAAGAA CCCAGCTTAT 
TATGGCTTTT CTGAAGGATT AGACAAGATT GGAATTGAAG CAAAATTGCA AGAATTGTGC 
TTAAAAAATC TAAATGATTT GTCTTCTCTG GGATTAATTA AAATGGATGA AGAAATTAAT 
TTTAAACCCA CAGAAACTGG AAAACTGATG GCTCTGTATT ACATTGCATT TAACACAGCA 
AAATTGTTTC ATACAATTAG AGGAACAGAA ACACTAGCTG AATTGGTGTC ACTGATATCA 
TCATGTTCAG AATTCTCAGA TGTGCAGCTA AGAGCAAATG AAAGAAGAGT TTTGAACACT 
TTAAATAAAG ACAAGAACAG AGTAACTATC AGATATCCAA TGGAAGGGAA GATTAAAACC 
AAAGAAATGA AAGTTAACTG CCTTATTCAA GCTCATCTTG GGTGCATTCT TGTTCAAGAC 
TTCTCTCTGA CACAAGATAT TTCAAAGATT TTCAGGCATG GCACACGATT GGCTAAATGT 
TTATCAGAGT TTCTTGCATT GCAAGAAAAC AAGTTCTCTG CATTTCTAAA TGCACTAATT 
TTGACCAAGT GTTTCAAAAG CAAACTTTGG GAAAATTCAT CACACATTTC TAAGCAACTG 
GAAAAAATTG GTGTCACTCT GGCAAATGCA ATGGTAAATG CAGGACTTAC AACATTTAAA 
AAAATAGAAG ATACAAATGC CAGAGAACTT GAATTGATTG TCAATAGGCA CCCACCCTTT 
GGAAATCAAA TAAAAGAATC TGTTGCACAC CTTCCAAAGT ATGAAATAAA GTTCCAGCAG 
CTAGCAAAGT ACAGAGCTAC AACTGCAGAA GTTGTTTTGA CTGTTCTCTT GACAAATTTC 
AAGCAGCTGC AGAAGAAAAG AACAGCTCCA GATTCACATT TTGTAATGCT TGTTATTGGA 
GATGATGATA ATCAAGCTAT CTTCAAACAG AAAATTATGG ACTCCTCTCT TTTTAAAACT 
GGAAGTTGGA CAAGAAAAAT TGAAATCAAA AGAGCATCTA AATCAACTAA TGTTTGCTTG 
AATCTCATCA GCTCAGAATA TGTTGGCCTT GACATCCAAC AAAGTTTCAA CATTTGCTAT 
CTGGGATCAG ACAGTTTTAA TGCAGATCTG GTGACTCAGC ATAAATCTGA AAATATATTT 
TCACAAGAAA TATATAAAAC TTCCACAAAA ACTAAAGATA TTGCTGATAC TAATTGGTCT 
ATGAGACAGA CAGCCATAAA ATGTGCTACT AGAGAATGTA ATCATAATTG CAAGAATAAA 
GATGCCTGTG GACATGAATG CTGTAAAGTT GGAGTGTCTG AGAAGGCTGA GATGAAGACA 
AACTTCTATT CTTACCTAAA TGATCTAAGG ACTCGAAATT CAGTTTCATC AGCTACACCA 
GTGAAAAGAC TAAAGATGCA GATGGAAGCT GAAACTAGGA ATGTGAATTT ACAGAACTTT 
GTCTTCACGC CAAAACCTTC AATAAGGTCA CCATGTCTGA AAATTCCTGA AAACATTTTA 
CTTCAAGCAG AGTCTTCTGT TTTGACAAAC AAATATAAAG CTTCACATCA AGAAGAAACA 
TGTTGTAAAT ATGAAATTAA AGGTTGCGGA GAAAAGCAAA CAAAAGGGAT GCCAAAAAAA 
ACATGTGAAA GGAGAAATCT CACTGATTCA GACAAATGCA ACTCATCGTC ATCAGAGAAC 
GCAGCATACG TGACATTTGA CTTGGGCAAT GACATATGGG ATGATTTTGA TGATGATAAT 
CTTTTAGATG CTAGTAATAT TTCAAGTTTA ACTGACCAAC ACACTTCAGA ATTTACAGAC 
GTCTCGTTCA GTAAGAACCA ACCTGTATCA AAGTCCTCAT TGTCAGCAGG ACACCATGAA 
GAAGCAAGAT ACAATGCAAT GAGTATAAAC AAGGATTCCT ATAGATATTT AAATGCTAAG 
TCAGAAGTTT TTTCTCAAGA AAACACCCAG TGCATTGATG TTGTACCATC AAATCAAAAA 
TTACATGGAA AGATGTTTTC TGAGAATACT ATAAAACATA TGTTTACCCT TCAAGAAGAA 
ACCAATTACC CTGTTACCTC CAAAATGGAT TCTCCACACA ATGGTTGCTG GTCAAGTAAC 
ATTTTTTTCA AGACTCCAGA AAATAAAACA CAAGAGAGAG GATGTTATCA TCAGGAAGAT 
GAAACAAATG CTTTCATTGG AATATTTAAT GGAATATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hfm1 ( XM_018093091.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948580.2 Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X2, mRNA.

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CloneID	OXa56157
Clone ID Related Accession (Same CDS sequence)	XM_018093091.2
Accession Version	XM_018093091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent DNA helicase HFM1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC CAGTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGAGGT AATGGCACTA 
AAGAAGTTTC AGCAACCATT TAAAGAAAGT AATGAAACAG AAAGTAAAGC TTCTGTTTCA 
TGTGCCTCCC AGTGTGGAAA CAAAACAAAA AAAACAAGCT TGGAGGGCAT GACATTACCA 
TTTTCATGGA TTACAAATGA TGTTTCCCAA TGTATAGATT TAACGCAGGA TGAACATATC 
TGTTCATTAA GCCCAGTTAC AGATACTGAG GAAAATATGA GTGCATTCAA AAAAAGCTTG 
TTTGGGAAAT ATGATTGTTC TTTAAACAGT GAAGACCAAT GTCTTATAGT GGAAAATGGG 
AGTTCTAATT TTGTAACCTC AAAAGAAGCA CAAAAAGAAG CAATGAAAGC AGATTTTGAC 
ATATGCCCAC CTCAGCAAAT GGCTTTCGAT TTATTTAATG AAACTGTAGT TCAAAAGGAC 
ATGCTGAATA AGGAGAAATT TTCTCACTCT GCTTCTTCAA ATATCTCTAA CAAAGATGAT 
GCACAATATG GAATTGTTAC ACCTGTCACA GAAATCCCTA AGCAATTCCG GACTGTCTTC 
AAAGAATTTC CATACTTTAA TTATATACAG TCGAAAGCAT TGGAGCATCT TCTTTATTCA 
GACAGAAACT TTGTGCTTTG TGCTCCAACT GGATCTGGAA AGACAGTTAT ATTTGAACTT 
GCAATTATTC GATTGCTAAT GCAAGTGCCA ATGCCTTGGA CAAATGTTAA AATAGTATAC 
ATGGCACCAA TCAAAGCTTT ATGTGGTCAG AGATATGATG ACTGGAAAGC AAAATTTGGA 
CCAGTTGGTT TGAATTGCAA AGAGTTAACT GGTGATACTG AAATGGATGA TCTTTTTGAA 
ATTCAGCATG CACACATCAT CATGACAACT CCAGAAAAAT GGGATAGTAT GACGAGAAAA 
TGGAAAGATA ATACTTTGGT TCAGCTTGTA CGTTTGTTTC TGATTGATGA GGTGCATATT 
TTGAAGGAAG AAAATCGAGG TGCAACACTT GAGGTTGTCG TCAGCAGAAT GAAAACAATC 
TATTCTCTTT CTCATTTGTC AGAAGATAGA AAGGCATTCA TACCGATGAG ATTTGTGGCA 
GTTTCTGCAA CCATTCCTAA TGTGGAAGAT ATAGCGGATT GGTTGTCGGA TGAAAACAGT 
CCTGGTGTAT GCATGAAAAT GGATGAATCA AGCAGACCAG TGAAACTGCG CAAAGTGGTG 
TTAGGCTTTC CTTGCAGCAC CAAGCAGTCA GAGTTCAAGT TTGATCTAAC ACTAAATTAC 
AAAATAGCTA ATATAATACA AACCTATTCA GATGGGAGAC CAACCCTTGT GTTTTGTTCT 
ACAAGGAAAG GAGTACAACA AGCAGCGTCT ATTCTAACCA AAGATGCAAA GTTTGTAATG 
AGCATTGAAC ACAAGCAGAG GCTTCAGAAA TGTGCTAATT CAATTAAAGA TTCTAAGCTA 
AGAGATGTTT TACAATATGG TGTTGGTTAC CACCATGCTG GTGTTGATAT ATCTGACAGG 
AAAGTCATTG AAAACTCTTT TTTAATTGGA GATCTTCCAG TACTTTTCAC CACAAGTACC 
TTAGCTATGG GAGTAAACCT TCCAGCTCAT TTAGTTATTG TAAAATCAAC AATGCATTAT 
GTCTCTGGAA TGTTTCAAGA ATATAGTGAG ACAGATATTC TTCAGATGAT TGGAAGAGCT 
GGAAGACCTC AGTTTGATTC TACAGCCACA GCCGTCATTA TGACCAGATT AAGCACCAAA 
GAAAAATATG TACACATGTT AGATGGTGCA GATACAATAG AAAGCAGCTT GCACAAGCAT 
CTTGTTGAGC ATCTGAATGC AGAAATAGCA CTGCACACCA TTACAGATGT TAAAGTTGCT 
TTGGAGTGGA TAAGATCGAC TTTCTTGTAT ATCAGAGCGC TGAAGAACCC AGCTTATTAT 
GGCTTTTCTG AAGGATTAGA CAAGATTGGA ATTGAAGCAA AATTGCAAGA ATTGTGCTTA 
AAAAATCTAA ATGATTTGTC TTCTCTGGGA TTAATTAAAA TGGATGAAGA AATTAATTTT 
AAACCCACAG AAACTGGAAA ACTGATGGCT CTGTATTACA TTGCATTTAA CACAGCAAAA 
TTGTTTCATA CAATTAGAGG AACAGAAACA CTAGCTGAAT TGGTGTCACT GATATCATCA 
TGTTCAGAAT TCTCAGATGT GCAGCTAAGA GCAAATGAAA GAAGAGTTTT GAACACTTTA 
AATAAAGACA AGAACAGAGT AACTATCAGA TATCCAATGG AAGGGAAGAT TAAAACCAAA 
GAAATGAAAG TTAACTGCCT TATTCAAGCT CATCTTGGGT GCATTCTTGT TCAAGACTTC 
TCTCTGACAC AAGATATTTC AAAGATTTTC AGGCATGGCA CACGATTGGC TAAATGTTTA 
TCAGAGTTTC TTGCATTGCA AGAAAACAAG TTCTCTGCAT TTCTAAATGC ACTAATTTTG 
ACCAAGTGTT TCAAAAGCAA ACTTTGGGAA AATTCATCAC ACATTTCTAA GCAACTGGAA 
AAAATTGGTG TCACTCTGGC AAATGCAATG GTAAATGCAG GACTTACAAC ATTTAAAAAA 
ATAGAAGATA CAAATGCCAG AGAACTTGAA TTGATTGTCA ATAGGCACCC ACCCTTTGGA 
AATCAAATAA AAGAATCTGT TGCACACCTT CCAAAGTATG AAATAAAGTT CCAGCAGCTA 
GCAAAGTACA GAGCTACAAC TGCAGAAGTT GTTTTGACTG TTCTCTTGAC AAATTTCAAG 
CAGCTGCAGA AGAAAAGAAC AGCTCCAGAT TCACATTTTG TAATGCTTGT TATTGGAGAT 
GATGATAATC AAGCTATCTT CAAACAGAAA ATTATGGACT CCTCTCTTTT TAAAACTGGA 
AGTTGGACAA GAAAAATTGA AATCAAAAGA GCATCTAAAT CAACTAATGT TTGCTTGAAT 
CTCATCAGCT CAGAATATGT TGGCCTTGAC ATCCAACAAA GTTTCAACAT TTGCTATCTG 
GGATCAGACA GTTTTAATGC AGATCTGGTG ACTCAGCATA AATCTGAAAA TATATTTTCA 
CAAGAAATAT ATAAAACTTC CACAAAAACT AAAGATATTG CTGATACTAA TTGGTCTATG 
AGACAGACAG CCATAAAATG TGCTACTAGA GAATGTAATC ATAATTGCAA GAATAAAGAT 
GCCTGTGGAC ATGAATGCTG TAAAGTTGGA GTGTCTGAGA AGGCTGAGAT GAAGACAAAC 
TTCTATTCTT ACCTAAATGA TCTAAGGACT CGAAATTCAG TTTCATCAGC TACACCAGTG 
AAAAGACTAA AGATGCAGAT GGAAGCTGAA ACTAGGAATG TGAATTTACA GAACTTTGTC 
TTCACGCCAA AACCTTCAAT AAGGTCACCA TGTCTGAAAA TTCCTGAAAA CATTTTACTT 
CAAGCAGAGT CTTCTGTTTT GACAAACAAA TATAAAGCTT CACATCAAGA AGAAACATGT 
TGTAAATATG AAATTAAAGG TTGCGGAGAA AAGCAAACAA AAGGGATGCC AAAAAAAACA 
TGTGAAAGGA GAAATCTCAC TGATTCAGAC AAATGCAACT CATCGTCATC AGAGAACGCA 
GCATACGTGA CATTTGACTT GGGCAATGAC ATATGGGATG ATTTTGATGA TGATAATCTT 
TTAGATGCTA GTAATATTTC AAGTTTAACT GACCAACACA CTTCAGAATT TACAGACGTC 
TCGTTCAGTA AGAACCAACC TGTATCAAAG TCCTCATTGT CAGCAGGACA CCATGAAGAA 
GCAAGATACA ATGCAATGAG TATAAACAAG GATTCCTATA GATATTTAAA TGCTAAGTCA 
GAAGTTTTTT CTCAAGAAAA CACCCAGTGC ATTGATGTTG TACCATCAAA TCAAAAATTA 
CATGGAAAGA TGTTTTCTGA GAATACTATA AAACATATGT TTACCCTTCA AGAAGAAACC 
AATTACCCTG TTACCTCCAA AATGGATTCT CCACACAATG GTTGCTGGTC AAGTAACATT 
TTTTTCAAGA CTCCAGAAAA TAAAACACAA GAGAGAGGAT GTTATCATCA GGAAGATGAA 
ACAAATGCTT TCATTGGAAT ATTTAATGGA ATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948580.2
CDS	108..4406
Translation	METPVNSLHSLNGLFSEVPDVSVLQKEELKMWLKAPAPGIAEIPLTQDLQNELSTEVMALKKFQQPFKESNETESKASVSCASQCGNKTKKTSLEGMTLPFSWITNDVSQCIDLTQDEHICSLSPVTDTEENMSAFKKSLFGKYDCSLNSEDQCLIVENGSSNFVTSKEAQKEAMKADFDICPPQQMAFDLFNETVVQKDMLNKEKFSHSASSNISNKDDAQYGIVTPVTEIPKQFRTVFKEFPYFNYIQSKALEHLLYSDRNFVLCAPTGSGKTVIFELAIIRLLMQVPMPWTNVKIVYMAPIKALCGQRYDDWKAKFGPVGLNCKELTGDTEMDDLFEIQHAHIIMTTPEKWDSMTRKWKDNTLVQLVRLFLIDEVHILKEENRGATLEVVVSRMKTIYSLSHLSEDRKAFIPMRFVAVSATIPNVEDIADWLSDENSPGVCMKMDESSRPVKLRKVVLGFPCSTKQSEFKFDLTLNYKIANIIQTYSDGRPTLVFCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLDGADTIESSLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEINFKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELVSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQENKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAPDSHFVMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLGSDSFNADLVTQHKSENIFSQEIYKTSTKTKDIADTNWSMRQTAIKCATRECNHNCKNKDACGHECCKVGVSEKAEMKTNFYSYLNDLRTRNSVSSATPVKRLKMQMEAETRNVNLQNFVFTPKPSIRSPCLKIPENILLQAESSVLTNKYKASHQEETCCKYEIKGCGEKQTKGMPKKTCERRNLTDSDKCNSSSSENAAYVTFDLGNDIWDDFDDDNLLDASNISSLTDQHTSEFTDVSFSKNQPVSKSSLSAGHHEEARYNAMSINKDSYRYLNAKSEVFSQENTQCIDVVPSNQKLHGKMFSENTIKHMFTLQEETNYPVTSKMDSPHNGCWSSNIFFKTPENKTQERGCYHQEDETNAFIGIFNGIF

Target ORF information:

RefSeq Version	XM_018093091.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093091.2

ORF Insert Sequence:

ATGGAGACAC CAGTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGAGGT AATGGCACTA 
AAGAAGTTTC AGCAACCATT TAAAGAAAGT AATGAAACAG AAAGTAAAGC TTCTGTTTCA 
TGTGCCTCCC AGTGTGGAAA CAAAACAAAA AAAACAAGCT TGGAGGGCAT GACATTACCA 
TTTTCATGGA TTACAAATGA TGTTTCCCAA TGTATAGATT TAACGCAGGA TGAACATATC 
TGTTCATTAA GCCCAGTTAC AGATACTGAG GAAAATATGA GTGCATTCAA AAAAAGCTTG 
TTTGGGAAAT ATGATTGTTC TTTAAACAGT GAAGACCAAT GTCTTATAGT GGAAAATGGG 
AGTTCTAATT TTGTAACCTC AAAAGAAGCA CAAAAAGAAG CAATGAAAGC AGATTTTGAC 
ATATGCCCAC CTCAGCAAAT GGCTTTCGAT TTATTTAATG AAACTGTAGT TCAAAAGGAC 
ATGCTGAATA AGGAGAAATT TTCTCACTCT GCTTCTTCAA ATATCTCTAA CAAAGATGAT 
GCACAATATG GAATTGTTAC ACCTGTCACA GAAATCCCTA AGCAATTCCG GACTGTCTTC 
AAAGAATTTC CATACTTTAA TTATATACAG TCGAAAGCAT TGGAGCATCT TCTTTATTCA 
GACAGAAACT TTGTGCTTTG TGCTCCAACT GGATCTGGAA AGACAGTTAT ATTTGAACTT 
GCAATTATTC GATTGCTAAT GCAAGTGCCA ATGCCTTGGA CAAATGTTAA AATAGTATAC 
ATGGCACCAA TCAAAGCTTT ATGTGGTCAG AGATATGATG ACTGGAAAGC AAAATTTGGA 
CCAGTTGGTT TGAATTGCAA AGAGTTAACT GGTGATACTG AAATGGATGA TCTTTTTGAA 
ATTCAGCATG CACACATCAT CATGACAACT CCAGAAAAAT GGGATAGTAT GACGAGAAAA 
TGGAAAGATA ATACTTTGGT TCAGCTTGTA CGTTTGTTTC TGATTGATGA GGTGCATATT 
TTGAAGGAAG AAAATCGAGG TGCAACACTT GAGGTTGTCG TCAGCAGAAT GAAAACAATC 
TATTCTCTTT CTCATTTGTC AGAAGATAGA AAGGCATTCA TACCGATGAG ATTTGTGGCA 
GTTTCTGCAA CCATTCCTAA TGTGGAAGAT ATAGCGGATT GGTTGTCGGA TGAAAACAGT 
CCTGGTGTAT GCATGAAAAT GGATGAATCA AGCAGACCAG TGAAACTGCG CAAAGTGGTG 
TTAGGCTTTC CTTGCAGCAC CAAGCAGTCA GAGTTCAAGT TTGATCTAAC ACTAAATTAC 
AAAATAGCTA ATATAATACA AACCTATTCA GATGGGAGAC CAACCCTTGT GTTTTGTTCT 
ACAAGGAAAG GAGTACAACA AGCAGCGTCT ATTCTAACCA AAGATGCAAA GTTTGTAATG 
AGCATTGAAC ACAAGCAGAG GCTTCAGAAA TGTGCTAATT CAATTAAAGA TTCTAAGCTA 
AGAGATGTTT TACAATATGG TGTTGGTTAC CACCATGCTG GTGTTGATAT ATCTGACAGG 
AAAGTCATTG AAAACTCTTT TTTAATTGGA GATCTTCCAG TACTTTTCAC CACAAGTACC 
TTAGCTATGG GAGTAAACCT TCCAGCTCAT TTAGTTATTG TAAAATCAAC AATGCATTAT 
GTCTCTGGAA TGTTTCAAGA ATATAGTGAG ACAGATATTC TTCAGATGAT TGGAAGAGCT 
GGAAGACCTC AGTTTGATTC TACAGCCACA GCCGTCATTA TGACCAGATT AAGCACCAAA 
GAAAAATATG TACACATGTT AGATGGTGCA GATACAATAG AAAGCAGCTT GCACAAGCAT 
CTTGTTGAGC ATCTGAATGC AGAAATAGCA CTGCACACCA TTACAGATGT TAAAGTTGCT 
TTGGAGTGGA TAAGATCGAC TTTCTTGTAT ATCAGAGCGC TGAAGAACCC AGCTTATTAT 
GGCTTTTCTG AAGGATTAGA CAAGATTGGA ATTGAAGCAA AATTGCAAGA ATTGTGCTTA 
AAAAATCTAA ATGATTTGTC TTCTCTGGGA TTAATTAAAA TGGATGAAGA AATTAATTTT 
AAACCCACAG AAACTGGAAA ACTGATGGCT CTGTATTACA TTGCATTTAA CACAGCAAAA 
TTGTTTCATA CAATTAGAGG AACAGAAACA CTAGCTGAAT TGGTGTCACT GATATCATCA 
TGTTCAGAAT TCTCAGATGT GCAGCTAAGA GCAAATGAAA GAAGAGTTTT GAACACTTTA 
AATAAAGACA AGAACAGAGT AACTATCAGA TATCCAATGG AAGGGAAGAT TAAAACCAAA 
GAAATGAAAG TTAACTGCCT TATTCAAGCT CATCTTGGGT GCATTCTTGT TCAAGACTTC 
TCTCTGACAC AAGATATTTC AAAGATTTTC AGGCATGGCA CACGATTGGC TAAATGTTTA 
TCAGAGTTTC TTGCATTGCA AGAAAACAAG TTCTCTGCAT TTCTAAATGC ACTAATTTTG 
ACCAAGTGTT TCAAAAGCAA ACTTTGGGAA AATTCATCAC ACATTTCTAA GCAACTGGAA 
AAAATTGGTG TCACTCTGGC AAATGCAATG GTAAATGCAG GACTTACAAC ATTTAAAAAA 
ATAGAAGATA CAAATGCCAG AGAACTTGAA TTGATTGTCA ATAGGCACCC ACCCTTTGGA 
AATCAAATAA AAGAATCTGT TGCACACCTT CCAAAGTATG AAATAAAGTT CCAGCAGCTA 
GCAAAGTACA GAGCTACAAC TGCAGAAGTT GTTTTGACTG TTCTCTTGAC AAATTTCAAG 
CAGCTGCAGA AGAAAAGAAC AGCTCCAGAT TCACATTTTG TAATGCTTGT TATTGGAGAT 
GATGATAATC AAGCTATCTT CAAACAGAAA ATTATGGACT CCTCTCTTTT TAAAACTGGA 
AGTTGGACAA GAAAAATTGA AATCAAAAGA GCATCTAAAT CAACTAATGT TTGCTTGAAT 
CTCATCAGCT CAGAATATGT TGGCCTTGAC ATCCAACAAA GTTTCAACAT TTGCTATCTG 
GGATCAGACA GTTTTAATGC AGATCTGGTG ACTCAGCATA AATCTGAAAA TATATTTTCA 
CAAGAAATAT ATAAAACTTC CACAAAAACT AAAGATATTG CTGATACTAA TTGGTCTATG 
AGACAGACAG CCATAAAATG TGCTACTAGA GAATGTAATC ATAATTGCAA GAATAAAGAT 
GCCTGTGGAC ATGAATGCTG TAAAGTTGGA GTGTCTGAGA AGGCTGAGAT GAAGACAAAC 
TTCTATTCTT ACCTAAATGA TCTAAGGACT CGAAATTCAG TTTCATCAGC TACACCAGTG 
AAAAGACTAA AGATGCAGAT GGAAGCTGAA ACTAGGAATG TGAATTTACA GAACTTTGTC 
TTCACGCCAA AACCTTCAAT AAGGTCACCA TGTCTGAAAA TTCCTGAAAA CATTTTACTT 
CAAGCAGAGT CTTCTGTTTT GACAAACAAA TATAAAGCTT CACATCAAGA AGAAACATGT 
TGTAAATATG AAATTAAAGG TTGCGGAGAA AAGCAAACAA AAGGGATGCC AAAAAAAACA 
TGTGAAAGGA GAAATCTCAC TGATTCAGAC AAATGCAACT CATCGTCATC AGAGAACGCA 
GCATACGTGA CATTTGACTT GGGCAATGAC ATATGGGATG ATTTTGATGA TGATAATCTT 
TTAGATGCTA GTAATATTTC AAGTTTAACT GACCAACACA CTTCAGAATT TACAGACGTC 
TCGTTCAGTA AGAACCAACC TGTATCAAAG TCCTCATTGT CAGCAGGACA CCATGAAGAA 
GCAAGATACA ATGCAATGAG TATAAACAAG GATTCCTATA GATATTTAAA TGCTAAGTCA 
GAAGTTTTTT CTCAAGAAAA CACCCAGTGC ATTGATGTTG TACCATCAAA TCAAAAATTA 
CATGGAAAGA TGTTTTCTGA GAATACTATA AAACATATGT TTACCCTTCA AGAAGAAACC 
AATTACCCTG TTACCTCCAA AATGGATTCT CCACACAATG GTTGCTGGTC AAGTAACATT 
TTTTTCAAGA CTCCAGAAAA TAAAACACAA GAGAGAGGAT GTTATCATCA GGAAGATGAA 
ACAAATGCTT TCATTGGAAT ATTTAATGGA ATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hfm1 ( XM_031900197.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756057.1 Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X3, mRNA.

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CloneID	OXa56158
Clone ID Related Accession (Same CDS sequence)	XM_031900197.1
Accession Version	XM_031900197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent DNA helicase HFM1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC CAGTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGCAGA GGTAATGGCA 
CTAAAGAAGT TTCAGCAACC ATTTAAAGAA AGTAATGAAA CAGAAAGTAA AGCTTCTGTT 
TCATGTGCCT CCCAGTGTGG AAACAAAACA AAAAAAACAA GCTTGGAGGG CATGACATTA 
CCATTTTCAT GGATTACAAA TGATGTTTCC CAATGTATAG ATTTAACGCA GGATGAACAT 
ATCTGTTCAT TAAGCCCAGT TACAGATACT GAGGAAAATA TGAGTGCATT CAAAAAAAGC 
TTGTTTGGGA AATATGATTG TTCTTTAAAC AGTGAAGACC AATGTCTTAT AGTGGAAAAT 
GGGAGTTCTA ATTTTGTAAC CTCAAAAGAA GCACAAAAAG AAGCAATGAA AGCAGATTTT 
GACATATGCC CACCTCAGCA AATGGCTTTC GATTTATTTA ATGAAACTGT AGTTCAAAAG 
GACATGCTGA ATAAGGAGAA ATTTTCTCAC TCTGCTTCTT CAAATATCTC TAACAAAGAT 
GATGCACAAT ATGGAATTGT TACACCTGTC ACAGAAATCC CTAAGCAATT CCGGACTGTC 
TTCAAAGAAT TTCCATACTT TAATTATATA CAGTCGAAAG CATTGGAGCA TCTTCTTTAT 
TCAGACAGAA ACTTTGTGCT TTGTGCTCCA ACTGGATCTG GAAAGACAGT TATATTTGAA 
CTTGCAATTA TTCGATTGCT AATGCAAGTG CCAATGCCTT GGACAAATGT TAAAATAGTA 
TACATGGCAC CAATCAAAGC TTTATGTGGT CAGAGATATG ATGACTGGAA AGCAAAATTT 
GGACCAGTTG GTTTGAATTG CAAAGAGTTA ACTGGTGATA CTGAAATGGA TGATCTTTTT 
GAAATTCAGC ATGCACACAT CATCATGACA ACTCCAGAAA AATGGGATAG TATGACGAGA 
AAATGGAAAG ATAATACTTT GGTTCAGCTT GTACGTTTGT TTCTGATTGA TGAGGTGCAT 
ATTTTGAAGG AAGAAAATCG AGGTGCAACA CTTGAGGTTG TCGTCAGCAG AATGAAAACA 
ATCTATTCTC TTTCTCATTT GTCAGAAGAT AGAAAGGCAT TCATACCGAT GAGATTTGTG 
GCAGTTTCTG CAACCATTCC TAATGTGGAA GATATAGCGG ATTGGTTGTC GGATGAAAAC 
AGTCCTGGTG TATGCATGAA AATGGATGAA TCAAGCAGAC CAGTGAAACT GCGCAAAGTG 
GTGTTAGGCT TTCCTTGCAG CACCAAGCAG TCAGAGTTCA AGTTTGATCT AACACTAAAT 
TACAAAATAG CTAATATAAT ACAAACCTAT TCAGATGGGA GACCAACCCT TGTGTTTTGT 
TCTACAAGGA AAGGAGTACA ACAAGCAGCG TCTATTCTAA CCAAAGATGC AAAGTTTGTA 
ATGAGCATTG AACACAAGCA GAGGCTTCAG AAATGTGCTA ATTCAATTAA AGATTCTAAG 
CTAAGAGATG TTTTACAATA TGGTGTTGGT TACCACCATG CTGGTGTTGA TATATCTGAC 
AGGAAAGTCA TTGAAAACTC TTTTTTAATT GGAGATCTTC CAGTACTTTT CACCACAAGT 
ACCTTAGCTA TGGGAGTAAA CCTTCCAGCT CATTTAGTTA TTGTAAAATC AACAATGCAT 
TATGTCTCTG GAATGTTTCA AGAATATAGT GAGACAGATA TTCTTCAGAT GATTGGAAGA 
GCTGGAAGAC CTCAGTTTGA TTCTACAGCC ACAGCCGTCA TTATGACCAG ATTAAGCACC 
AAAGAAAAAT ATGTACACAT GTTAGATGGT GCAGATACAA TAGAAAGCAG CTTGCACAAG 
CATCTTGTTG AGCATCTGAA TGCAGAAATA GCACTGCACA CCATTACAGA TGTTAAAGTT 
GCTTTGGAGT GGATAAGATC GACTTTCTTG TATATCAGAG CGCTGAAGAA CCCAGCTTAT 
TATGGCTTTT CTGAAGGATT AGACAAGATT GGAATTGAAG CAAAATTGCA AGAATTGTGC 
TTAAAAAATC TAAATGATTT GTCTTCTCTG GGATTAATTA AAATGGATGA AGAAATTAAT 
TTTAAACCCA CAGAAACTGG AAAACTGATG GCTCTGTATT ACATTGCATT TAACACAGCA 
AAATTGTTTC ATACAATTAG AGGAACAGAA ACACTAGCTG AATTGGTGTC ACTGATATCA 
TCATGTTCAG AATTCTCAGA TGTGCAGCTA AGAGCAAATG AAAGAAGAGT TTTGAACACT 
TTAAATAAAG ACAAGAACAG AGTAACTATC AGATATCCAA TGGAAGGGAA GATTAAAACC 
AAAGAAATGA AAGTTAACTG CCTTATTCAA GCTCATCTTG GGTGCATTCT TGTTCAAGAC 
TTCTCTCTGA CACAAGATAT TTCAAAGATT TTCAGGCATG GCACACGATT GGCTAAATGT 
GTCACTCTGG CAAATGCAAT GGTAAATGCA GGACTTACAA CATTTAAAAA AATAGAAGAT 
ACAAATGCCA GAGAACTTGA ATTGATTGTC AATAGGCACC CACCCTTTGG AAATCAAATA 
AAAGAATCTG TTGCACACCT TCCAAAGTAT GAAATAAAGT TCCAGCAGCT AGCAAAGTAC 
AGAGCTACAA CTGCAGAAGT TGTTTTGACT GTTCTCTTGA CAAATTTCAA GCAGCTGCAG 
AAGAAAAGAA CAGCTCCAGA TTCACATTTT GTAATGCTTG TTATTGGAGA TGATGATAAT 
CAAGCTATCT TCAAACAGAA AATTATGGAC TCCTCTCTTT TTAAAACTGG AAGTTGGACA 
AGAAAAATTG AAATCAAAAG AGCATCTAAA TCAACTAATG TTTGCTTGAA TCTCATCAGC 
TCAGAATATG TTGGCCTTGA CATCCAACAA AGTTTCAACA TTTGCTATCT GGGATCAGAC 
AGTTTTAATG CAGATCTGGT GACTCAGCAT AAATCTGAAA ATATATTTTC ACAAGAAATA 
TATAAAACTT CCACAAAAAC TAAAGATATT GCTGATACTA ATTGGTCTAT GAGACAGACA 
GCCATAAAAT GTGCTACTAG AGAATGTAAT CATAATTGCA AGAATAAAGA TGCCTGTGGA 
CATGAATGCT GTAAAGTTGG AGTGTCTGAG AAGGCTGAGA TGAAGACAAA CTTCTATTCT 
TACCTAAATG ATCTAAGGAC TCGAAATTCA GTTTCATCAG CTACACCAGT GAAAAGACTA 
AAGATGCAGA TGGAAGCTGA AACTAGGAAT GTGAATTTAC AGAACTTTGT CTTCACGCCA 
AAACCTTCAA TAAGGTCACC ATGTCTGAAA ATTCCTGAAA ACATTTTACT TCAAGCAGAG 
TCTTCTGTTT TGACAAACAA ATATAAAGCT TCACATCAAG AAGAAACATG TTGTAAATAT 
GAAATTAAAG GTTGCGGAGA AAAGCAAACA AAAGGGATGC CAAAAAAAAC ATGTGAAAGG 
AGAAATCTCA CTGATTCAGA CAAATGCAAC TCATCGTCAT CAGAGAACGC AGCATACGTG 
ACATTTGACT TGGGCAATGA CATATGGGAT GATTTTGATG ATGATAATCT TTTAGATGCT 
AGTAATATTT CAAGTTTAAC TGACCAACAC ACTTCAGAAT TTACAGACGT CTCGTTCAGT 
AAGAACCAAC CTGTATCAAA GTCCTCATTG TCAGCAGGAC ACCATGAAGA AGCAAGATAC 
AATGCAATGA GTATAAACAA GGATTCCTAT AGATATTTAA ATGCTAAGTC AGAAGTTTTT 
TCTCAAGAAA ACACCCAGTG CATTGATGTT GTACCATCAA ATCAAAAATT ACATGGAAAG 
ATGTTTTCTG AGAATACTAT AAAACATATG TTTACCCTTC AAGAAGAAAC CAATTACCCT 
GTTACCTCCA AAATGGATTC TCCACACAAT GGTTGCTGGT CAAGTAACAT TTTTTTCAAG 
ACTCCAGAAA ATAAAACACA AGAGAGAGGA TGTTATCATC AGGAAGATGA AACAAATGCT 
TTCATTGGAA TATTTAATGG AATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756057.1
CDS	99..4268
Translation	METPVNSLHSLNGLFSEVPDVSVLQKEELKMWLKAPAPGIAEIPLTQDLQNELSTAEVMALKKFQQPFKESNETESKASVSCASQCGNKTKKTSLEGMTLPFSWITNDVSQCIDLTQDEHICSLSPVTDTEENMSAFKKSLFGKYDCSLNSEDQCLIVENGSSNFVTSKEAQKEAMKADFDICPPQQMAFDLFNETVVQKDMLNKEKFSHSASSNISNKDDAQYGIVTPVTEIPKQFRTVFKEFPYFNYIQSKALEHLLYSDRNFVLCAPTGSGKTVIFELAIIRLLMQVPMPWTNVKIVYMAPIKALCGQRYDDWKAKFGPVGLNCKELTGDTEMDDLFEIQHAHIIMTTPEKWDSMTRKWKDNTLVQLVRLFLIDEVHILKEENRGATLEVVVSRMKTIYSLSHLSEDRKAFIPMRFVAVSATIPNVEDIADWLSDENSPGVCMKMDESSRPVKLRKVVLGFPCSTKQSEFKFDLTLNYKIANIIQTYSDGRPTLVFCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLDGADTIESSLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEINFKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELVSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAPDSHFVMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLGSDSFNADLVTQHKSENIFSQEIYKTSTKTKDIADTNWSMRQTAIKCATRECNHNCKNKDACGHECCKVGVSEKAEMKTNFYSYLNDLRTRNSVSSATPVKRLKMQMEAETRNVNLQNFVFTPKPSIRSPCLKIPENILLQAESSVLTNKYKASHQEETCCKYEIKGCGEKQTKGMPKKTCERRNLTDSDKCNSSSSENAAYVTFDLGNDIWDDFDDDNLLDASNISSLTDQHTSEFTDVSFSKNQPVSKSSLSAGHHEEARYNAMSINKDSYRYLNAKSEVFSQENTQCIDVVPSNQKLHGKMFSENTIKHMFTLQEETNYPVTSKMDSPHNGCWSSNIFFKTPENKTQERGCYHQEDETNAFIGIFNGIF

Target ORF information:

RefSeq Version	XM_031900197.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900197.1

ORF Insert Sequence:

ATGGAGACAC CAGTCAATTC TTTACATTCC TTGAATGGAT TGTTTTCTGA GGTGCCAGAT 
GTTAGTGTGT TGCAAAAGGA AGAACTTAAA ATGTGGCTTA AGGCACCAGC TCCGGGCATT 
GCTGAAATTC CACTTACTCA GGATCTTCAA AATGAGTTAT CAACAGCAGA GGTAATGGCA 
CTAAAGAAGT TTCAGCAACC ATTTAAAGAA AGTAATGAAA CAGAAAGTAA AGCTTCTGTT 
TCATGTGCCT CCCAGTGTGG AAACAAAACA AAAAAAACAA GCTTGGAGGG CATGACATTA 
CCATTTTCAT GGATTACAAA TGATGTTTCC CAATGTATAG ATTTAACGCA GGATGAACAT 
ATCTGTTCAT TAAGCCCAGT TACAGATACT GAGGAAAATA TGAGTGCATT CAAAAAAAGC 
TTGTTTGGGA AATATGATTG TTCTTTAAAC AGTGAAGACC AATGTCTTAT AGTGGAAAAT 
GGGAGTTCTA ATTTTGTAAC CTCAAAAGAA GCACAAAAAG AAGCAATGAA AGCAGATTTT 
GACATATGCC CACCTCAGCA AATGGCTTTC GATTTATTTA ATGAAACTGT AGTTCAAAAG 
GACATGCTGA ATAAGGAGAA ATTTTCTCAC TCTGCTTCTT CAAATATCTC TAACAAAGAT 
GATGCACAAT ATGGAATTGT TACACCTGTC ACAGAAATCC CTAAGCAATT CCGGACTGTC 
TTCAAAGAAT TTCCATACTT TAATTATATA CAGTCGAAAG CATTGGAGCA TCTTCTTTAT 
TCAGACAGAA ACTTTGTGCT TTGTGCTCCA ACTGGATCTG GAAAGACAGT TATATTTGAA 
CTTGCAATTA TTCGATTGCT AATGCAAGTG CCAATGCCTT GGACAAATGT TAAAATAGTA 
TACATGGCAC CAATCAAAGC TTTATGTGGT CAGAGATATG ATGACTGGAA AGCAAAATTT 
GGACCAGTTG GTTTGAATTG CAAAGAGTTA ACTGGTGATA CTGAAATGGA TGATCTTTTT 
GAAATTCAGC ATGCACACAT CATCATGACA ACTCCAGAAA AATGGGATAG TATGACGAGA 
AAATGGAAAG ATAATACTTT GGTTCAGCTT GTACGTTTGT TTCTGATTGA TGAGGTGCAT 
ATTTTGAAGG AAGAAAATCG AGGTGCAACA CTTGAGGTTG TCGTCAGCAG AATGAAAACA 
ATCTATTCTC TTTCTCATTT GTCAGAAGAT AGAAAGGCAT TCATACCGAT GAGATTTGTG 
GCAGTTTCTG CAACCATTCC TAATGTGGAA GATATAGCGG ATTGGTTGTC GGATGAAAAC 
AGTCCTGGTG TATGCATGAA AATGGATGAA TCAAGCAGAC CAGTGAAACT GCGCAAAGTG 
GTGTTAGGCT TTCCTTGCAG CACCAAGCAG TCAGAGTTCA AGTTTGATCT AACACTAAAT 
TACAAAATAG CTAATATAAT ACAAACCTAT TCAGATGGGA GACCAACCCT TGTGTTTTGT 
TCTACAAGGA AAGGAGTACA ACAAGCAGCG TCTATTCTAA CCAAAGATGC AAAGTTTGTA 
ATGAGCATTG AACACAAGCA GAGGCTTCAG AAATGTGCTA ATTCAATTAA AGATTCTAAG 
CTAAGAGATG TTTTACAATA TGGTGTTGGT TACCACCATG CTGGTGTTGA TATATCTGAC 
AGGAAAGTCA TTGAAAACTC TTTTTTAATT GGAGATCTTC CAGTACTTTT CACCACAAGT 
ACCTTAGCTA TGGGAGTAAA CCTTCCAGCT CATTTAGTTA TTGTAAAATC AACAATGCAT 
TATGTCTCTG GAATGTTTCA AGAATATAGT GAGACAGATA TTCTTCAGAT GATTGGAAGA 
GCTGGAAGAC CTCAGTTTGA TTCTACAGCC ACAGCCGTCA TTATGACCAG ATTAAGCACC 
AAAGAAAAAT ATGTACACAT GTTAGATGGT GCAGATACAA TAGAAAGCAG CTTGCACAAG 
CATCTTGTTG AGCATCTGAA TGCAGAAATA GCACTGCACA CCATTACAGA TGTTAAAGTT 
GCTTTGGAGT GGATAAGATC GACTTTCTTG TATATCAGAG CGCTGAAGAA CCCAGCTTAT 
TATGGCTTTT CTGAAGGATT AGACAAGATT GGAATTGAAG CAAAATTGCA AGAATTGTGC 
TTAAAAAATC TAAATGATTT GTCTTCTCTG GGATTAATTA AAATGGATGA AGAAATTAAT 
TTTAAACCCA CAGAAACTGG AAAACTGATG GCTCTGTATT ACATTGCATT TAACACAGCA 
AAATTGTTTC ATACAATTAG AGGAACAGAA ACACTAGCTG AATTGGTGTC ACTGATATCA 
TCATGTTCAG AATTCTCAGA TGTGCAGCTA AGAGCAAATG AAAGAAGAGT TTTGAACACT 
TTAAATAAAG ACAAGAACAG AGTAACTATC AGATATCCAA TGGAAGGGAA GATTAAAACC 
AAAGAAATGA AAGTTAACTG CCTTATTCAA GCTCATCTTG GGTGCATTCT TGTTCAAGAC 
TTCTCTCTGA CACAAGATAT TTCAAAGATT TTCAGGCATG GCACACGATT GGCTAAATGT 
GTCACTCTGG CAAATGCAAT GGTAAATGCA GGACTTACAA CATTTAAAAA AATAGAAGAT 
ACAAATGCCA GAGAACTTGA ATTGATTGTC AATAGGCACC CACCCTTTGG AAATCAAATA 
AAAGAATCTG TTGCACACCT TCCAAAGTAT GAAATAAAGT TCCAGCAGCT AGCAAAGTAC 
AGAGCTACAA CTGCAGAAGT TGTTTTGACT GTTCTCTTGA CAAATTTCAA GCAGCTGCAG 
AAGAAAAGAA CAGCTCCAGA TTCACATTTT GTAATGCTTG TTATTGGAGA TGATGATAAT 
CAAGCTATCT TCAAACAGAA AATTATGGAC TCCTCTCTTT TTAAAACTGG AAGTTGGACA 
AGAAAAATTG AAATCAAAAG AGCATCTAAA TCAACTAATG TTTGCTTGAA TCTCATCAGC 
TCAGAATATG TTGGCCTTGA CATCCAACAA AGTTTCAACA TTTGCTATCT GGGATCAGAC 
AGTTTTAATG CAGATCTGGT GACTCAGCAT AAATCTGAAA ATATATTTTC ACAAGAAATA 
TATAAAACTT CCACAAAAAC TAAAGATATT GCTGATACTA ATTGGTCTAT GAGACAGACA 
GCCATAAAAT GTGCTACTAG AGAATGTAAT CATAATTGCA AGAATAAAGA TGCCTGTGGA 
CATGAATGCT GTAAAGTTGG AGTGTCTGAG AAGGCTGAGA TGAAGACAAA CTTCTATTCT 
TACCTAAATG ATCTAAGGAC TCGAAATTCA GTTTCATCAG CTACACCAGT GAAAAGACTA 
AAGATGCAGA TGGAAGCTGA AACTAGGAAT GTGAATTTAC AGAACTTTGT CTTCACGCCA 
AAACCTTCAA TAAGGTCACC ATGTCTGAAA ATTCCTGAAA ACATTTTACT TCAAGCAGAG 
TCTTCTGTTT TGACAAACAA ATATAAAGCT TCACATCAAG AAGAAACATG TTGTAAATAT 
GAAATTAAAG GTTGCGGAGA AAAGCAAACA AAAGGGATGC CAAAAAAAAC ATGTGAAAGG 
AGAAATCTCA CTGATTCAGA CAAATGCAAC TCATCGTCAT CAGAGAACGC AGCATACGTG 
ACATTTGACT TGGGCAATGA CATATGGGAT GATTTTGATG ATGATAATCT TTTAGATGCT 
AGTAATATTT CAAGTTTAAC TGACCAACAC ACTTCAGAAT TTACAGACGT CTCGTTCAGT 
AAGAACCAAC CTGTATCAAA GTCCTCATTG TCAGCAGGAC ACCATGAAGA AGCAAGATAC 
AATGCAATGA GTATAAACAA GGATTCCTAT AGATATTTAA ATGCTAAGTC AGAAGTTTTT 
TCTCAAGAAA ACACCCAGTG CATTGATGTT GTACCATCAA ATCAAAAATT ACATGGAAAG 
ATGTTTTCTG AGAATACTAT AAAACATATG TTTACCCTTC AAGAAGAAAC CAATTACCCT 
GTTACCTCCA AAATGGATTC TCCACACAAT GGTTGCTGGT CAAGTAACAT TTTTTTCAAG 
ACTCCAGAAA ATAAAACACA AGAGAGAGGA TGTTATCATC AGGAAGATGA AACAAATGCT 
TTCATTGGAA TATTTAATGG AATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hfm1 ( NM_001078915.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072383.3 Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), mRNA.

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CloneID	OXa01166
Clone ID Related Accession (Same CDS sequence)	NM_001078915.2
Accession Version	NM_001078915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent DNA helicase HFM1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC133060.1. On Feb 11, 2007 this sequence version replaced NM_001078915.1. ##Evidence-Data-START## Transcript exon combination :: BC133060.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGGCTTA AGGCACCAGC TCCGGGCATT GCTGAAATTC CACTTACTCA GGATCTTCAA 
AATGAGTTAT CAACAGAGGT AATGGCACTA AAGAAGTTTC AGCAACCATT TAAAGAAAGT 
AATGAAACAG AAAGTAAAGC TTCTGTTTCA TGTGCCTCCC AGTGTGGAAA CAAAACAAAA 
AAAACAAGCT TGGAGGGCAT GACATTACCA TTTTCATGGA TTACAAATGA TGTTTCCCAA 
TGTATAGATT TAACGCAGGA TGAACATATC TGTTCATTAA GCCCAGTTAC AGATACTGAG 
GAAAATATGA GTGCATTCAA AAAAAGCTTG TTTGGGAAAT ATGATTGTTC TTTAAACAGT 
GAAGACCAAT GTCTTATAGT GGAAAATGGG AGTTCTAATT TTGTAACCTC AAAAGAAGCA 
CAAAAAGAAG CAATGAAAGC AGATTCTGAC ATATGCCCAC CTCAGCAAAT GGCTTTCAAT 
TTATTTAATG AAACTGTAGT TCAAAAGGAC ATGCTGAATA AGGAGAAATT TTCTCACTCT 
GCTTCTTCAA ATATCTCTAA CAAAGATGAT GCACAATATG GAATTGTTAC ACCTGTCACA 
GAAATCCCTA AGCAATTCCG GACTGTCTTC AAAGAATTTC CATACTTTAA TTATATACAG 
TCGAAAGCAT TGGAGCATCT TCTTTATTCA GACAGAAACT TTGTGCTTTG TGCTCCAACT 
GGATCTGGAA AGACAGTTAT ATTTGAACTT GCAATTATTC GATTGCTAAT GCAAGTGCCA 
ATGCCTTGGA CAAATGTTAA AATAGTATAC ATGGCACCAA TCAAAGCTTT ATGTGGTCAG 
AGATATGATG ACTGGAAAGC AAAATTTGGA CCAGTTGGTT TGAATTGCAA AGAGTTAACT 
GGTGATACTG AAATGGATGA TCTTTTTGAA ATTCAGCATG CACACATCAT CATGACAACT 
CCAGAAAAAT GGGATAGTAT GACGAGAAAA TGGAAAGATA ATACTTTGGT TCAGCTTGTA 
CGTTTGTTTC TGATTGATGA GGTGCATATT TTGAAGGAAG AAAATCGAGG TGCAACACTT 
GAGGTTGTCG TCAGCAGAAT GAAAACAATC TATTCTCTTT CTCATTTGTC AGAAGATAGA 
AAGGCATTCA TACCGATGAG ATTTGTGGCA GTTTCTGCAA CCATTCCTAA TGTGGAAGAT 
ATAGCAGATT GGTTGTCGGA TGAAAACAGT CCTGGTGTAT GCATGAAAAT GGATGAATCA 
AGCAGACCAG TGAAACTGCG CAAAGTGGTG TTAGGCTTTC CTTGCAGCAC CAAGCAGTCA 
GAGTTCAAGT TTGATCTAAC ACTAAATTAC AAAATAGCTA ATATAATACA AACCTATTCA 
GATGGGAGAC CAACCCTTGT GTTTTGTTCT ACAAGGAAAG GAGTACAACA AGCAGCGTCT 
ATTCTAACCA AAGATGCAAA GTTTGTAATG AGCATTGAAC ACAAGCAGAG GCTTCAGAAA 
TGTGCTAATT CAATTAAAGA TTCTAAGCTA AGAGATGTTT TACAATATGG TGTTGGTTAC 
CACCATGCTG GTGTTGATAT ATCTGACAGG AAAGTCATTG AAAACTCTTT TTTAATTGGA 
GATCTTCCAG TACTTTTCAC CACAAGTACC TTAGCTATGG GAGTAAACCT TCCAGCTCAT 
TTAGTTATTG TAAAATCAAC AATGCATTAT GTCTCTGGAA TGTTTCAAGA ATATAGTGAG 
ACAGATATTC TTCAGATGAT TGGAAGAGCT GGAAGACCTC AGTTTGATTC TACAGCCACA 
GCCGTCATTA TGACCAGATT AAGCACCAAA GAAAAATATG TACACATGTT AGATGGTGCA 
GATACAATAG AAAGCAGCTT GCACAAGCAT CTTGTTGAGC ATCTGAATGC AGAAATAGCA 
CTGCACACCA TTACAGATGT TAAAGTTGCT TTGGAGTGGA TAAGATCGAC TTTCTTGTAT 
ATCAGAGCGC TGAAGAACCC AGCTTATTAT GGCTTTTCTG AAGGATTAGA CAAGATTGGA 
ATTGAAGCAA AATTGCAAGA ATTGTGCTTA AAAAATCTAA ATGATTTGTC TTCTCTGGGA 
TTAATTAAAA TGGATGAAGA AATTAATTTT AAACCCACAG AAACTGGAAA ACTGATGGCT 
CTGTATTACA TTGCATTTAA CACAGCAAAA TTGTTTCATA CAATTAGAGG AACAGAAACA 
CTAGCTGAAT TGGTGTCACT GATATCATCA TGTTCAGAAT TCTCAGATGT GCAGCTAAGA 
GCAAATGAAA GAAGAGTTTT GAACACTTTA AATAAAGACA AGAACAGAGT AACTATTAGA 
TATCCAATGG AAGGGAAGAT TAAAACCAAA GAAATGAAAG TTAACTGCCT TATTCAAGCT 
CATCTTGGGT GCATTCTTGT TCAAGACTTC TCTCTGACAC AAGATATTTC AAAGATTTTC 
AGGCATGGCA CACGATTGGC TAAATGTTTA TCAGAGTTTC TTGCATTGCA AGAAAACAAG 
TTCTCTGCAT TTCTAAATGC ACTAATTTTG ACCAAGTGTT TCAAAAGCAA ACTTTGGGAA 
AATTCATCAC ACATTTCTAA GCAACTGGAA AAAATTGGTG TCACTCTGGC AAATGCAATG 
GTAAATGCAG GACTTACAAC ATTTAAAAAA ATAGAAGATA CAAATGCCAG AGAACTTGAA 
TTGATTGTCA ATAGGCACCC ACCCTTTGGA AATCAAATAA AAGAATCTGT TGCACACCTT 
CCAAAGTATG AAATAAAGTT CCAGCAGCTA GCAAAGTACA GAGCTACAAC TGCAGAAGTT 
GTTTTGACTG TTCTCTTGAC AAATTTCAAG CAGCTGCAGA AGAAAAGAAC AGCTCCAGAT 
TCACATTTTG TAATGCTTGT TATTGGAGAT GATGATAATC AAGCTATCTT CAAACAGAAA 
ATTATGGACT CCTCTCTTTT TAAAACTGGA AGTTGGACAA GAAAAATTGA AATCAAAAGA 
GCATCTAAAT CAACTAATGT TTGCTTGAAT CTCATCAGCT CAGAATATGT TGGCCTTGAC 
ATCCAACAAA GTTTCAACAT TTGCTATCTG GGATCAGACA GTTTTAATGC AGATCTGGTG 
ACTCAGCATA AATCTGAAAA TATATTTTCA CAAGAAATAT ATAAAACTTC CACAAAAACT 
AAAGATATTG CTGATACTAA TTGGTCTATG AGACAGACAG CCATAAAATG TGCTACTAGA 
GAATGTAATC ATAATTGCAA GAATAAAGAT GCCTGTGGAC ATGAATGCTG TAAAGTTGGA 
GTGTCTGAGA AGGCTGAGAT GAAGACAAAC TTCTATTCTT ACCTAAATGA TCTAAGGACT 
CGAAATTCAG TTTCATCAGC TACACCAGTG AAAAGACTAA AGATGCAGAT GGAAGCTGAA 
ACTAGGAATG TGAATTTACA GAACTTTGTC TTCACGCCAA AACCTTCAAT AAGGTCACCA 
TGTCTGAAAA TTCCTGAAAA CATTTTACTT CAAGCAGAGT CTTCTGTTTT GACAAACAAA 
TATAAAGCTT CACATCAAGA AGAAACATGT TGTAAATATG AAATTAAAGG TTGCGGAGAA 
AAGCAAACAA AAGGGATGCC AAAAAAAACA TGTGAAAGGA GAAATCTCAC TGATTCAGAC 
AAATGCAACT CATCGTCATC AGAGAACGCA GCATACGTGA CATTTGACTT GGGCAATGAC 
ATATGGGATG ATTTTGATGA TGATAATCTT TTAGATGCTA GTAATATTTC AAGTTTAACT 
GACCAACACA CTTCAGAATT TACAGACGTC TCGTTCAGTA AGAACCAACC TGTATCAAAG 
TCCTCATTGT CAGCAGGACA CCATGAAGAA GCAAGATACA ATGCAATGAG TATAAACAAG 
GATTCCTATA GATATTTAAA TGCTAAGTCA GAAGTTTTTT CTCAAGAAAA CACCCAGTGC 
ATTGATGTTG TACCATCAAA TCAAAAATTA CATGGAAAGA TGTTTTCTGA GAATACTATA 
AAACATATGT TTACCCTTCA AGAAGAAACC AATTACCCTG TTACCTCCAA AATGGATTCT 
CCACACAATG GTTGCTGGTC AAGTAACATT TTTTTCAAGA CTCCAGAAAA TAAAACACAA 
GAGAGAGGAT GTTATCATCA GGAAGATGAA ACAAATGCTT TCATTGGAAT ATTTAATGGA 
ATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072383.3
CDS	58..4266
Translation	MWLKAPAPGIAEIPLTQDLQNELSTEVMALKKFQQPFKESNETESKASVSCASQCGNKTKKTSLEGMTLPFSWITNDVSQCIDLTQDEHICSLSPVTDTEENMSAFKKSLFGKYDCSLNSEDQCLIVENGSSNFVTSKEAQKEAMKADSDICPPQQMAFNLFNETVVQKDMLNKEKFSHSASSNISNKDDAQYGIVTPVTEIPKQFRTVFKEFPYFNYIQSKALEHLLYSDRNFVLCAPTGSGKTVIFELAIIRLLMQVPMPWTNVKIVYMAPIKALCGQRYDDWKAKFGPVGLNCKELTGDTEMDDLFEIQHAHIIMTTPEKWDSMTRKWKDNTLVQLVRLFLIDEVHILKEENRGATLEVVVSRMKTIYSLSHLSEDRKAFIPMRFVAVSATIPNVEDIADWLSDENSPGVCMKMDESSRPVKLRKVVLGFPCSTKQSEFKFDLTLNYKIANIIQTYSDGRPTLVFCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLDGADTIESSLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEINFKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELVSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQENKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAPDSHFVMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLGSDSFNADLVTQHKSENIFSQEIYKTSTKTKDIADTNWSMRQTAIKCATRECNHNCKNKDACGHECCKVGVSEKAEMKTNFYSYLNDLRTRNSVSSATPVKRLKMQMEAETRNVNLQNFVFTPKPSIRSPCLKIPENILLQAESSVLTNKYKASHQEETCCKYEIKGCGEKQTKGMPKKTCERRNLTDSDKCNSSSSENAAYVTFDLGNDIWDDFDDDNLLDASNISSLTDQHTSEFTDVSFSKNQPVSKSSLSAGHHEEARYNAMSINKDSYRYLNAKSEVFSQENTQCIDVVPSNQKLHGKMFSENTIKHMFTLQEETNYPVTSKMDSPHNGCWSSNIFFKTPENKTQERGCYHQEDETNAFIGIFNGIF

Target ORF information:

RefSeq Version	NM_001078915.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HFM1, ATP-dependent DNA helicase homolog (hfm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078915.2

ORF Insert Sequence:

ATGTGGCTTA AGGCACCAGC TCCGGGCATT GCTGAAATTC CACTTACTCA GGATCTTCAA 
AATGAGTTAT CAACAGAGGT AATGGCACTA AAGAAGTTTC AGCAACCATT TAAAGAAAGT 
AATGAAACAG AAAGTAAAGC TTCTGTTTCA TGTGCCTCCC AGTGTGGAAA CAAAACAAAA 
AAAACAAGCT TGGAGGGCAT GACATTACCA TTTTCATGGA TTACAAATGA TGTTTCCCAA 
TGTATAGATT TAACGCAGGA TGAACATATC TGTTCATTAA GCCCAGTTAC AGATACTGAG 
GAAAATATGA GTGCATTCAA AAAAAGCTTG TTTGGGAAAT ATGATTGTTC TTTAAACAGT 
GAAGACCAAT GTCTTATAGT GGAAAATGGG AGTTCTAATT TTGTAACCTC AAAAGAAGCA 
CAAAAAGAAG CAATGAAAGC AGATTCTGAC ATATGCCCAC CTCAGCAAAT GGCTTTCAAT 
TTATTTAATG AAACTGTAGT TCAAAAGGAC ATGCTGAATA AGGAGAAATT TTCTCACTCT 
GCTTCTTCAA ATATCTCTAA CAAAGATGAT GCACAATATG GAATTGTTAC ACCTGTCACA 
GAAATCCCTA AGCAATTCCG GACTGTCTTC AAAGAATTTC CATACTTTAA TTATATACAG 
TCGAAAGCAT TGGAGCATCT TCTTTATTCA GACAGAAACT TTGTGCTTTG TGCTCCAACT 
GGATCTGGAA AGACAGTTAT ATTTGAACTT GCAATTATTC GATTGCTAAT GCAAGTGCCA 
ATGCCTTGGA CAAATGTTAA AATAGTATAC ATGGCACCAA TCAAAGCTTT ATGTGGTCAG 
AGATATGATG ACTGGAAAGC AAAATTTGGA CCAGTTGGTT TGAATTGCAA AGAGTTAACT 
GGTGATACTG AAATGGATGA TCTTTTTGAA ATTCAGCATG CACACATCAT CATGACAACT 
CCAGAAAAAT GGGATAGTAT GACGAGAAAA TGGAAAGATA ATACTTTGGT TCAGCTTGTA 
CGTTTGTTTC TGATTGATGA GGTGCATATT TTGAAGGAAG AAAATCGAGG TGCAACACTT 
GAGGTTGTCG TCAGCAGAAT GAAAACAATC TATTCTCTTT CTCATTTGTC AGAAGATAGA 
AAGGCATTCA TACCGATGAG ATTTGTGGCA GTTTCTGCAA CCATTCCTAA TGTGGAAGAT 
ATAGCAGATT GGTTGTCGGA TGAAAACAGT CCTGGTGTAT GCATGAAAAT GGATGAATCA 
AGCAGACCAG TGAAACTGCG CAAAGTGGTG TTAGGCTTTC CTTGCAGCAC CAAGCAGTCA 
GAGTTCAAGT TTGATCTAAC ACTAAATTAC AAAATAGCTA ATATAATACA AACCTATTCA 
GATGGGAGAC CAACCCTTGT GTTTTGTTCT ACAAGGAAAG GAGTACAACA AGCAGCGTCT 
ATTCTAACCA AAGATGCAAA GTTTGTAATG AGCATTGAAC ACAAGCAGAG GCTTCAGAAA 
TGTGCTAATT CAATTAAAGA TTCTAAGCTA AGAGATGTTT TACAATATGG TGTTGGTTAC 
CACCATGCTG GTGTTGATAT ATCTGACAGG AAAGTCATTG AAAACTCTTT TTTAATTGGA 
GATCTTCCAG TACTTTTCAC CACAAGTACC TTAGCTATGG GAGTAAACCT TCCAGCTCAT 
TTAGTTATTG TAAAATCAAC AATGCATTAT GTCTCTGGAA TGTTTCAAGA ATATAGTGAG 
ACAGATATTC TTCAGATGAT TGGAAGAGCT GGAAGACCTC AGTTTGATTC TACAGCCACA 
GCCGTCATTA TGACCAGATT AAGCACCAAA GAAAAATATG TACACATGTT AGATGGTGCA 
GATACAATAG AAAGCAGCTT GCACAAGCAT CTTGTTGAGC ATCTGAATGC AGAAATAGCA 
CTGCACACCA TTACAGATGT TAAAGTTGCT TTGGAGTGGA TAAGATCGAC TTTCTTGTAT 
ATCAGAGCGC TGAAGAACCC AGCTTATTAT GGCTTTTCTG AAGGATTAGA CAAGATTGGA 
ATTGAAGCAA AATTGCAAGA ATTGTGCTTA AAAAATCTAA ATGATTTGTC TTCTCTGGGA 
TTAATTAAAA TGGATGAAGA AATTAATTTT AAACCCACAG AAACTGGAAA ACTGATGGCT 
CTGTATTACA TTGCATTTAA CACAGCAAAA TTGTTTCATA CAATTAGAGG AACAGAAACA 
CTAGCTGAAT TGGTGTCACT GATATCATCA TGTTCAGAAT TCTCAGATGT GCAGCTAAGA 
GCAAATGAAA GAAGAGTTTT GAACACTTTA AATAAAGACA AGAACAGAGT AACTATTAGA 
TATCCAATGG AAGGGAAGAT TAAAACCAAA GAAATGAAAG TTAACTGCCT TATTCAAGCT 
CATCTTGGGT GCATTCTTGT TCAAGACTTC TCTCTGACAC AAGATATTTC AAAGATTTTC 
AGGCATGGCA CACGATTGGC TAAATGTTTA TCAGAGTTTC TTGCATTGCA AGAAAACAAG 
TTCTCTGCAT TTCTAAATGC ACTAATTTTG ACCAAGTGTT TCAAAAGCAA ACTTTGGGAA 
AATTCATCAC ACATTTCTAA GCAACTGGAA AAAATTGGTG TCACTCTGGC AAATGCAATG 
GTAAATGCAG GACTTACAAC ATTTAAAAAA ATAGAAGATA CAAATGCCAG AGAACTTGAA 
TTGATTGTCA ATAGGCACCC ACCCTTTGGA AATCAAATAA AAGAATCTGT TGCACACCTT 
CCAAAGTATG AAATAAAGTT CCAGCAGCTA GCAAAGTACA GAGCTACAAC TGCAGAAGTT 
GTTTTGACTG TTCTCTTGAC AAATTTCAAG CAGCTGCAGA AGAAAAGAAC AGCTCCAGAT 
TCACATTTTG TAATGCTTGT TATTGGAGAT GATGATAATC AAGCTATCTT CAAACAGAAA 
ATTATGGACT CCTCTCTTTT TAAAACTGGA AGTTGGACAA GAAAAATTGA AATCAAAAGA 
GCATCTAAAT CAACTAATGT TTGCTTGAAT CTCATCAGCT CAGAATATGT TGGCCTTGAC 
ATCCAACAAA GTTTCAACAT TTGCTATCTG GGATCAGACA GTTTTAATGC AGATCTGGTG 
ACTCAGCATA AATCTGAAAA TATATTTTCA CAAGAAATAT ATAAAACTTC CACAAAAACT 
AAAGATATTG CTGATACTAA TTGGTCTATG AGACAGACAG CCATAAAATG TGCTACTAGA 
GAATGTAATC ATAATTGCAA GAATAAAGAT GCCTGTGGAC ATGAATGCTG TAAAGTTGGA 
GTGTCTGAGA AGGCTGAGAT GAAGACAAAC TTCTATTCTT ACCTAAATGA TCTAAGGACT 
CGAAATTCAG TTTCATCAGC TACACCAGTG AAAAGACTAA AGATGCAGAT GGAAGCTGAA 
ACTAGGAATG TGAATTTACA GAACTTTGTC TTCACGCCAA AACCTTCAAT AAGGTCACCA 
TGTCTGAAAA TTCCTGAAAA CATTTTACTT CAAGCAGAGT CTTCTGTTTT GACAAACAAA 
TATAAAGCTT CACATCAAGA AGAAACATGT TGTAAATATG AAATTAAAGG TTGCGGAGAA 
AAGCAAACAA AAGGGATGCC AAAAAAAACA TGTGAAAGGA GAAATCTCAC TGATTCAGAC 
AAATGCAACT CATCGTCATC AGAGAACGCA GCATACGTGA CATTTGACTT GGGCAATGAC 
ATATGGGATG ATTTTGATGA TGATAATCTT TTAGATGCTA GTAATATTTC AAGTTTAACT 
GACCAACACA CTTCAGAATT TACAGACGTC TCGTTCAGTA AGAACCAACC TGTATCAAAG 
TCCTCATTGT CAGCAGGACA CCATGAAGAA GCAAGATACA ATGCAATGAG TATAAACAAG 
GATTCCTATA GATATTTAAA TGCTAAGTCA GAAGTTTTTT CTCAAGAAAA CACCCAGTGC 
ATTGATGTTG TACCATCAAA TCAAAAATTA CATGGAAAGA TGTTTTCTGA GAATACTATA 
AAACATATGT TTACCCTTCA AGAAGAAACC AATTACCCTG TTACCTCCAA AATGGATTCT 
CCACACAATG GTTGCTGGTC AAGTAACATT TTTTTCAAGA CTCCAGAAAA TAAAACACAA 
GAGAGAGGAT GTTATCATCA GGAAGATGAA ACAAATGCTT TCATTGGAAT ATTTAATGGA 
ATATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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hfm1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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