HFM1 cDNA ORF clone, Bos taurus(cattle)

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216307 NM_001205576.2
Latest version!
Bos taurus HFM1, ATP dependent DNA helicase homolog (HFM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa90437 XM_015464340.1
Latest version!
Bos taurus HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa40351 NM_001205576.3
Latest version!
Bos taurus helicase for meiosis 1 (HFM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa40351 NM_001205576.1 Bos taurus HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (HFM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216307
    Clone ID Related Accession (Same CDS sequence) NM_001205576.2
    Accession Version NM_001205576.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product probable ATP-dependent DNA helicase HFM1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02007913.1, DAAA02007915.1 and DAAA02007916.1. On Jun 30, 2017 this sequence version replaced NM_001205576.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN02403639 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCACGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTGTCCATTT ACAAGAAAGA AAACAACAAA GTCTGTCACC AGAGATTGAA
    AAGCTATGCT TTACTCGCTC TGAAAAAATA TCAAACTCTT CAAATTTTAA TGAGAAAGTG
    GATTGCTTTA TTAGAAATGA ATGTAAAAAG GAGATTGATT TCAGTAGGTA TCATCCTGAT
    GATGAAGCTG ACGAAATAAA GTCTTTTCTG GGAATATTTG ATGGTATCTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192505.2
    CDS1..4314
    Translation

    Target ORF information:

    RefSeq Version NM_001205576.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HFM1, ATP dependent DNA helicase homolog (HFM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205576.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCACGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTGTCCATTT ACAAGAAAGA AAACAACAAA GTCTGTCACC AGAGATTGAA
    AAGCTATGCT TTACTCGCTC TGAAAAAATA TCAAACTCTT CAAATTTTAA TGAGAAAGTG
    GATTGCTTTA TTAGAAATGA ATGTAAAAAG GAGATTGATT TCAGTAGGTA TCATCCTGAT
    GATGAAGCTG ACGAAATAAA GTCTTTTCTG GGAATATTTG ATGGTATCTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90437
    Clone ID Related Accession (Same CDS sequence) XM_015464340.1
    Accession Version XM_015464340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCACGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTATAAGAAG AAACACTTCT CTCTTTTTTC TTTTAAATAC TGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319826.1
    CDS40..4176
    Translation

    Target ORF information:

    RefSeq Version XM_015464340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCACGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTATAAGAAG AAACACTTCT CTCTTTTTTC TTTTAAATAC TGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40351
    Clone ID Related Accession (Same CDS sequence) NM_001205576.3 , NM_001205576.1
    Accession Version NM_001205576.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-07
    Organism Bos taurus(cattle)
    Product probable ATP-dependent DNA helicase HFM1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On May 30, 2018 this sequence version replaced NM_001205576.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145491 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTGTCCATTT ACAAGAAAGA AAACAACAAA GTCTGTCACC AGAGATTGAA
    AAGCTATGCT TTACTCGCTC TGAAAAAATA TCAAACTCTT CAAATTTTAA TGAGAAAGTG
    GATTGCTTTA TTAGAAATGA ATGTAAAAAG GAGATTGATT TCAGTAGGTA TCATCCTGAT
    GATGAAGCTG ACGAAATAAA GTCTTTTCTG GGAATATTTG ATGGTATCTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192505.3
    CDS1..4314
    Translation

    Target ORF information:

    RefSeq Version NM_001205576.3
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase for meiosis 1 (HFM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205576.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTGTCCATTT ACAAGAAAGA AAACAACAAA GTCTGTCACC AGAGATTGAA
    AAGCTATGCT TTACTCGCTC TGAAAAAATA TCAAACTCTT CAAATTTTAA TGAGAAAGTG
    GATTGCTTTA TTAGAAATGA ATGTAAAAAG GAGATTGATT TCAGTAGGTA TCATCCTGAT
    GATGAAGCTG ACGAAATAAA GTCTTTTCTG GGAATATTTG ATGGTATCTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40351
    Clone ID Related Accession (Same CDS sequence) NM_001205576.3 , NM_001205576.1
    Accession Version NM_001205576.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product probable ATP-dependent DNA helicase HFM1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03058781.1, AAFC03058779.1, AAFC03058780.1 and AAFC03058782.1. On Apr 20, 2011 this sequence version replaced XM_582165.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTGTCCATTT ACAAGAAAGA AAACAACAAA GTCTGTCACC AGAGATTGAA
    AAGCTATGCT TTACTCGCTC TGAAAAAATA TCAAACTCTT CAAATTTTAA TGAGAAAGTG
    GATTGCTTTA TTAGAAATGA ATGTAAAAAG GAGATTGATT TCAGTAGGTA TCATCCTGAT
    GATGAAGCTG ACGAAATAAA GTCTTTTCTG GGAATATTTG ATGGTATCTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192505.1
    CDS1..4314
    Translation

    Target ORF information:

    RefSeq Version NM_001205576.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (HFM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC CGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAATA TCTATGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG AAATAGAAAA TCATCAACTG
    TTAGGTCAGA ATAAAAAATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ATAAATTATG TTTTACAAAC TCAAAAATTC CAGTTTGACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGGT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGGAAAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAAGATGG TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATACTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCA GAGAAGGCAT AATCAAAGCA
    CCACTTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAAAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGACTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTAAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCCTGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACGATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCAGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATATACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCCAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATGAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACCG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCGGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATATAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTGATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAAATCTTA GCATAAATCT AATTAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCGAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGCCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GTAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CACAGAAGTC AGAAGGGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AATAAATCTC CAAGTGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTATAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACCATCTG CATATCAAGA CAAAGAAGTT
    TTGAAAGTGA ACTTTGAATT GGGAAATGAA ATTTGGGATG ACTTTGATGA TGAAAACTTA
    GTAGAAATTA CTAGCTTTTC AGCTGAAACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACTGG GGAAAGTAAG CTACCATTCC AAGAATCAAA AGGCCAGTTC
    CAAAGAGAAT CATCTAAGAG TTTTGTTTCA TCACATGAGA AGCCAGATAT TTATTTATCA
    AATTCTGCTG TGTCCAAGTT TGGTGCATCC TCCATGACAC AATTACCTCA ACAATCTGGA
    AATGCAAGTA TTGTCCATTT ACAAGAAAGA AAACAACAAA GTCTGTCACC AGAGATTGAA
    AAGCTATGCT TTACTCGCTC TGAAAAAATA TCAAACTCTT CAAATTTTAA TGAGAAAGTG
    GATTGCTTTA TTAGAAATGA ATGTAAAAAG GAGATTGATT TCAGTAGGTA TCATCCTGAT
    GATGAAGCTG ACGAAATAAA GTCTTTTCTG GGAATATTTG ATGGTATCTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.