HFM1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HFM1
Entrez Gene ID	557052
Full Name	HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae)
Synonyms	si:ch211-83f14.5
General protein information	Preferred Names HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) Names probable ATP-dependent DNA helicase HFM1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298501.3	XP_009296776.1	probable ATP-dependent DNA helicase HFM1 isoform X2
XM_009298502.2	XP_009296777.1	probable ATP-dependent DNA helicase HFM1 isoform X3
XM_009298502.1	XP_009296777.1	probable ATP-dependent DNA helicase HFM1 isoform X3
XM_021467172.1	XP_021322847.1	probable ATP-dependent DNA helicase HFM1 isoform X1
XM_009298501.2	XP_009296776.1	probable ATP-dependent DNA helicase HFM1 isoform X2
XM_017351616.1	XP_017207105.1	probable ATP-dependent DNA helicase HFM1 isoform X5
XM_017351609.1	XP_017207098.1	probable ATP-dependent DNA helicase HFM1 isoform X1
XM_009298503.1	XP_009296778.1	probable ATP-dependent DNA helicase HFM1 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	HFM1	NP_001017975.3
Danio rerio (zebrafish)	hfm1	XP_001919555.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009060	XP_552976.3
Arabidopsis thaliana (thale cress)	RCK	NP_189410.2
Xenopus tropicalis (tropical clawed frog)	hfm1	NP_001072383.3
Pan troglodytes (chimpanzee)	HFM1	XP_524618.3
Oryza sativa (rice)	Os02g0617500	NP_001047442.1
Rattus norvegicus (Norway rat)	Hfm1	NP_001178030.1
	LOC704516	XP_002808271.1
Canis lupus familiaris (dog)	HFM1	XP_005621996.1
Bos taurus (cattle)	HFM1	NP_001192505.1
Mus musculus (house mouse)	Hfm1	NP_808541.2
Gallus gallus (chicken)	HFM1	XP_422349.3

Gene Ontology

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42494	XM_009298503.1 Latest version!	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa56422	XM_017351616.1 Latest version!	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa42490	XM_021467172.1 Latest version!	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa56421	XM_017351609.1 Latest version!	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa42492	XM_009298501.3 Latest version!	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42493	XM_009298502.2 Latest version!	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa42493	XM_009298502.1	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42492	XM_009298501.2	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HFM1 ( XM_009298503.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296778.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa42494
Clone ID Related Accession (Same CDS sequence)	XM_009298503.1
Accession Version	XM_009298503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG 
CAACGACATG AAAGTGAATT GTCTGATACA AGCTCAGCTT GGCTGCATCC CCATTCAGGA 
GTTTGGACTC ATACAGGATA CTGGAAAAAT ATTCAGAAAT GCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296778.1
CDS	211..2658
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELVSNRREDQKQRHESELSDTSSAWLHPHSGVWTHTGYWKNIQKCS

Target ORF information:

RefSeq Version	XM_009298503.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298503.1

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG 
CAACGACATG AAAGTGAATT GTCTGATACA AGCTCAGCTT GGCTGCATCC CCATTCAGGA 
GTTTGGACTC ATACAGGATA CTGGAAAAAT ATTCAGAAAT GCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_017351616.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207105.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa56422
Clone ID Related Accession (Same CDS sequence)	XM_017351616.1
Accession Version	XM_017351616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG 
CAACGACATG AAAGTGAATT GGTGAAGTCT TGCTTTTGCT GTTTGATTGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207105.1
CDS	211..2604
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELVSNRREDQKQRHESELVKSCFCCLIV

Target ORF information:

RefSeq Version	XM_017351616.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351616.1

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGGTTTCCA ATAGAAGGGA AGATCAAAAG 
CAACGACATG AAAGTGAATT GGTGAAGTCT TGCTTTTGCT GTTTGATTGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_021467172.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021322847.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42490
Clone ID Related Accession (Same CDS sequence)	XM_021467172.1
Accession Version	XM_021467172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC 
TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA 
CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT 
GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG 
CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT 
CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA 
TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA 
TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAA 
GGTGAGCAAA GTTCTCTCGG ATTGGAAGGA CATGAGGAGA TGACATTTGA CAGTCAGCCT 
AAGCCACACA GTGATGGAAA CATGCCCAGG ATCACTCTTA ATAAAGCAAG AGTTTATTCA 
GACTTAAACG ACAGCACCGA CAGGACAGGT CTCAGGTCTG ATTTTCCTGA TGTGACCTTT 
GACCTGGGAG TAAACTATTG GGATGATTTT GATGACAGAA ACCTGGTTGA TGCAAGTAAT 
GTATGCTGTG AATCCCAACA ATTTAACCCG ATGATAGAGC ACCATCAGCC TGCACATTCT 
GGTTTGTCCA GTGGTTTGTC TCATTGTCCA GCACAAACGT TGCAAGAAAC TGTGAGTCCT 
GTCTTACCAC AAAGGGTGCA ACCAATGGCC GTTACTCCTC AAACTGCAAT GTGGACAAAC 
TTGAACACAA ACACCCTCTC AAGTGACCAG AGTCAAGCCA GTAAAGGTTC AGTCCAAAAA 
AGATTTGATT TTTTCTCAAG GACAGATGCA GCTTTAAAGG AAAACACACC TGTTGGCAGT 
GATCACACGA AGGAAATCAG CGATGAAGCC CATGTTTTTT TTGGTATGTT TGATGGCCTT 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322847.1
CDS	452..4837
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELIEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTEFLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEIVFTVNMNNFKELALKRTAPDHHYVTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYVGLDIQQDYSAHYSGVHGLQAEFSITEKPTWRFQRIHDTMKKRQGPTVATPNSSVNDILFVDQVAPNMVKRNCHHLCKNKELCRHDCCKIGVKRTVLQSNVSSYLDNLKMRNEKLCETPVKRLKMMMNNGTAAVSMQQFSYKPKDTHSAAKRFEGSQCYTQSAYQIQDLEASFATPSQNSSSEAWHDGYIDELGDGYTEHVNPALEHPQLSDLPQKGEQSSLGLEGHEEMTFDSQPKPHSDGNMPRITLNKARVYSDLNDSTDRTGLRSDFPDVTFDLGVNYWDDFDDRNLVDASNVCCESQQFNPMIEHHQPAHSGLSSGLSHCPAQTLQETVSPVLPQRVQPMAVTPQTAMWTNLNTNTLSSDQSQASKGSVQKRFDFFSRTDAALKENTPVGSDHTKEISDEAHVFFGMFDGLF

Target ORF information:

RefSeq Version	XM_021467172.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467172.1

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC 
TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA 
CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT 
GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG 
CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT 
CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA 
TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA 
TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAA 
GGTGAGCAAA GTTCTCTCGG ATTGGAAGGA CATGAGGAGA TGACATTTGA CAGTCAGCCT 
AAGCCACACA GTGATGGAAA CATGCCCAGG ATCACTCTTA ATAAAGCAAG AGTTTATTCA 
GACTTAAACG ACAGCACCGA CAGGACAGGT CTCAGGTCTG ATTTTCCTGA TGTGACCTTT 
GACCTGGGAG TAAACTATTG GGATGATTTT GATGACAGAA ACCTGGTTGA TGCAAGTAAT 
GTATGCTGTG AATCCCAACA ATTTAACCCG ATGATAGAGC ACCATCAGCC TGCACATTCT 
GGTTTGTCCA GTGGTTTGTC TCATTGTCCA GCACAAACGT TGCAAGAAAC TGTGAGTCCT 
GTCTTACCAC AAAGGGTGCA ACCAATGGCC GTTACTCCTC AAACTGCAAT GTGGACAAAC 
TTGAACACAA ACACCCTCTC AAGTGACCAG AGTCAAGCCA GTAAAGGTTC AGTCCAAAAA 
AGATTTGATT TTTTCTCAAG GACAGATGCA GCTTTAAAGG AAAACACACC TGTTGGCAGT 
GATCACACGA AGGAAATCAG CGATGAAGCC CATGTTTTTT TTGGTATGTT TGATGGCCTT 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_017351609.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207098.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56421
Clone ID Related Accession (Same CDS sequence)	XM_017351609.1
Accession Version	XM_017351609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC 
TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA 
CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT 
GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG 
CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT 
CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA 
TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA 
TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAG 
ATGACATTTG ACAGTCAGCC TAAGCCACAC AGTGATGGAA ACATGCCCAG GATCACTCTT 
AATAAAGCAA GAGTTTATTC AGACTTAAAC GACAGCACCG ACAGGACAGG TCTCAGGTCT 
GATTTTCCTG ATGTGACCTT TGACCTGGGA GTAAACTATT GGGATGATTT TGATGACAGA 
AACCTGGTTG ATGCAAGTAA TGTATGCTGT GAATCCCAAC AATTTAACCC GATGATAGAG 
CACCATCAGC CTGCACATTC TGGTTTGTCC AGTGGTTTGT CTCATTGTCC AGCACAAACG 
TTGCAAGAAA CTGTGAGTCC TGTCTTACCA CAAAGGGTGC AACCAATGGC CGTTACTCCT 
CAAACTGCAA TGTGGACAAA CTTGAACACA AACACCCTCT CAAGTGACCA GAGTCAAGCC 
AGTAAAGGTT CAGTCCAAAA AAGATTTGAT TTTTTCTCAA GGACAGATGC AGCTTTAAAG 
GAAAACACAC CTGTTGGCAG TGATCACACG AAGGAAATCA GCGATGAAGC CCATGTTTTT 
TTTGGTATGT TTGATGGCCT TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207098.1
CDS	211..4557
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELIEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTEFLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEIVFTVNMNNFKELALKRTAPDHHYVTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYVGLDIQQDYSAHYSGVHGLQAEFSITEKPTWRFQRIHDTMKKRQGPTVATPNSSVNDILFVDQVAPNMVKRNCHHLCKNKELCRHDCCKIGVKRTVLQSNVSSYLDNLKMRNEKLCETPVKRLKMMMNNGTAAVSMQQFSYKPKDTHSAAKRFEGSQCYTQSAYQIQDLEASFATPSQNSSSEAWHDGYIDELGDGYTEHVNPALEHPQLSDLPQKMTFDSQPKPHSDGNMPRITLNKARVYSDLNDSTDRTGLRSDFPDVTFDLGVNYWDDFDDRNLVDASNVCCESQQFNPMIEHHQPAHSGLSSGLSHCPAQTLQETVSPVLPQRVQPMAVTPQTAMWTNLNTNTLSSDQSQASKGSVQKRFDFFSRTDAALKENTPVGSDHTKEISDEAHVFFGMFDGLF

Target ORF information:

RefSeq Version	XM_017351609.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351609.1

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GACATTCTCT TCGTGGATCA AGTTGCTCCA AACATGGTTA AGAGAAATTG CCATCATCTC 
TGCAAAAACA AAGAGCTCTG TAGACATGAC TGCTGCAAAA TTGGAGTCAA AAGGACTGTA 
CTACAGTCAA ATGTCTCCTC ATACCTTGAC AACCTAAAAA TGAGGAATGA GAAACTTTGT 
GAGACTCCTG TGAAGCGATT GAAGATGATG ATGAATAATG GCACAGCAGC TGTAAGCATG 
CAGCAGTTCT CCTACAAACC CAAAGACACA CATTCTGCTG CCAAGAGGTT TGAAGGAAGT 
CAGTGTTACA CTCAGAGTGC TTATCAAATC CAGGATCTAG AGGCCAGTTT TGCCACTCCA 
TCCCAGAATT CATCATCTGA GGCATGGCAT GATGGTTACA TTGATGAGCT GGGAGATGGA 
TATACTGAAC ATGTAAACCC GGCATTAGAA CATCCTCAAC TGTCTGATTT ACCTCAAAAG 
ATGACATTTG ACAGTCAGCC TAAGCCACAC AGTGATGGAA ACATGCCCAG GATCACTCTT 
AATAAAGCAA GAGTTTATTC AGACTTAAAC GACAGCACCG ACAGGACAGG TCTCAGGTCT 
GATTTTCCTG ATGTGACCTT TGACCTGGGA GTAAACTATT GGGATGATTT TGATGACAGA 
AACCTGGTTG ATGCAAGTAA TGTATGCTGT GAATCCCAAC AATTTAACCC GATGATAGAG 
CACCATCAGC CTGCACATTC TGGTTTGTCC AGTGGTTTGT CTCATTGTCC AGCACAAACG 
TTGCAAGAAA CTGTGAGTCC TGTCTTACCA CAAAGGGTGC AACCAATGGC CGTTACTCCT 
CAAACTGCAA TGTGGACAAA CTTGAACACA AACACCCTCT CAAGTGACCA GAGTCAAGCC 
AGTAAAGGTT CAGTCCAAAA AAGATTTGAT TTTTTCTCAA GGACAGATGC AGCTTTAAAG 
GAAAACACAC CTGTTGGCAG TGATCACACG AAGGAAATCA GCGATGAAGC CCATGTTTTT 
TTTGGTATGT TTGATGGCCT TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_009298501.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296776.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.

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CloneID	ODa42492
Clone ID Related Accession (Same CDS sequence)	XM_009298501.3 , XM_009298501.2
Accession Version	XM_009298501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GGTCAGACTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296776.1
CDS	452..3766
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELIEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTEFLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEIVFTVNMNNFKELALKRTAPDHHYVTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYVGLDIQQDYSAHYSGVHGLQAEFSITEKPTWRFQRIHDTMKKRQGPTVATPNSSVNGQTQ

Target ORF information:

RefSeq Version	XM_009298501.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298501.3

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GGTCAGACTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_009298501.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296776.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.

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CloneID	ODa42492
Clone ID Related Accession (Same CDS sequence)	XM_009298501.3 , XM_009298501.2
Accession Version	XM_009298501.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GGTCAGACTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296776.1
CDS	211..3525
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELIEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTEFLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEIVFTVNMNNFKELALKRTAPDHHYVTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYVGLDIQQDYSAHYSGVHGLQAEFSITEKPTWRFQRIHDTMKKRQGPTVATPNSSVNGQTQ

Target ORF information:

RefSeq Version	XM_009298501.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298501.2

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGTGGGTCT GGACATACAG CAGGACTACA GCGCCCACTA CTCAGGAGTG 
CACGGTTTGC AAGCTGAGTT CTCCATTACA GAGAAACCAA CATGGAGATT TCAGAGGATT 
CATGACACAA TGAAGAAGAG ACAAGGCCCC ACAGTCGCAA CTCCCAATTC TTCAGTCAAC 
GGTCAGACTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_009298502.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296777.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42493
Clone ID Related Accession (Same CDS sequence)	XM_009298502.2 , XM_009298502.1
Accession Version	XM_009298502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296777.1
CDS	452..3589
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELIEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTEFLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEIVFTVNMNNFKELALKRTAPDHHYVTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYE

Target ORF information:

RefSeq Version	XM_009298502.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298502.2

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HFM1 ( XM_009298502.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296777.1 Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.

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CloneID	ODa42493
Clone ID Related Accession (Same CDS sequence)	XM_009298502.2 , XM_009298502.1
Accession Version	XM_009298502.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent DNA helicase HFM1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296777.1
CDS	211..3348
Translation	MVDSEESVSSLDNLFYDRPVIHKVKPLCQGQGSWVWDAPPSLSQIPSTQDIQREAAHASSFSFSQRSKTFLPPAQKPIHNPTEGIQGQTVQSHELEEPGLWSHEPSSTGEQAVFRMGFAETRPLPTASAPPFKTRLFQASTRRTSGNGEDGISNQTLSTTNDTAFQSQAPRRTSGPQAAHAVAACSPFIGLAQTEEKCDVPSSHSLHLGLKPAEFKGSSEKGTLRSVSEIPAKFRSVFKDFPYFNYVQSQALDDVLYSNKNFVACAPTGSGKTVLFELAIVRLLIEASEPWHNVRAVYMAPIKALCSQQYDNWKQKFGPLGLKCKELTGDTEIDDFFEIQDAHLIFTTAEKWDSMTRRWKDNCLLTSIRLFLVDEVHVVKDKTRGATLEVVVSRMKTMHFYRQAINPESKASVRFVAVSATIPNIQDVSEWLSDESGPATCLEMDESHRPVKLRKVVLGFPCGSNQNEFKFDLSLNYKLANIIQTYSDQKPTLVFCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMHFLSGLDCIESSLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHYGFPPEVDKCGIETKLQELCLKNLNSLASFNLITMDEDINIKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELIEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTEFLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEIVFTVNMNNFKELALKRTAPDHHYVTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYE

Target ORF information:

RefSeq Version	XM_009298502.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (hfm1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298502.1

ORF Insert Sequence:

ATGGTGGACT CAGAAGAAAG TGTATCATCA CTGGATAACC TGTTTTATGA CCGGCCAGTA 
ATTCATAAAG TGAAACCCTT ATGTCAAGGA CAAGGCTCCT GGGTTTGGGA TGCTCCACCA 
TCATTATCAC AAATACCTTC GACACAAGAC ATCCAGAGAG AGGCAGCACA CGCTTCTTCA 
TTTAGTTTCT CTCAGAGATC AAAGACATTT CTGCCTCCAG CACAAAAACC TATTCACAAT 
CCCACTGAAG GTATCCAAGG TCAGACTGTG CAATCACATG AGCTGGAGGA GCCCGGTCTA 
TGGAGCCATG AACCCTCTAG TACAGGAGAG CAGGCTGTTT TCAGAATGGG TTTTGCGGAA 
ACAAGGCCTT TACCTACTGC CAGTGCTCCT CCCTTTAAGA CTCGGCTGTT CCAAGCTTCC 
ACAAGAAGGA CTTCAGGAAA TGGAGAAGAC GGTATCTCAA ATCAAACATT GTCAACAACA 
AATGACACTG CTTTCCAAAG TCAGGCTCCT AGGAGAACCA GCGGTCCGCA GGCTGCACAT 
GCTGTTGCTG CATGTAGTCC CTTTATTGGT TTGGCCCAAA CTGAGGAAAA ATGTGATGTT 
CCCTCAAGCC ATAGTCTTCA TCTTGGTCTT AAACCTGCAG AATTTAAAGG GTCTTCTGAA 
AAAGGAACAC TTCGTTCAGT CTCTGAGATT CCTGCAAAAT TCAGAAGTGT GTTTAAAGAT 
TTCCCTTACT TCAATTATGT GCAGTCCCAG GCACTGGATG ATGTTCTGTA CTCCAATAAA 
AATTTTGTTG CCTGTGCTCC CACTGGCTCT GGAAAGACTG TGCTATTTGA GCTGGCCATT 
GTTCGACTGC TGATTGAGGC TTCAGAGCCT TGGCACAATG TCAGAGCAGT GTACATGGCA 
CCAATTAAGG CTCTCTGCAG CCAACAATAT GACAACTGGA AACAGAAGTT TGGACCTCTT 
GGACTTAAAT GCAAGGAACT AACTGGAGAC ACAGAGATTG ATGACTTTTT TGAAATCCAA 
GATGCTCATC TCATTTTTAC AACTGCAGAA AAATGGGATA GCATGACTAG AAGATGGAAG 
GATAACTGTT TATTGACATC CATTCGTCTC TTCCTCGTTG ATGAGGTACA TGTGGTGAAA 
GACAAAACCC GTGGAGCCAC TCTTGAGGTT GTGGTCAGCA GAATGAAAAC CATGCACTTC 
TACCGCCAAG CTATTAATCC TGAATCAAAA GCATCTGTGA GATTTGTAGC TGTCTCAGCT 
ACTATCCCTA ATATTCAAGA CGTATCTGAA TGGCTTTCAG ATGAGTCAGG GCCCGCCACG 
TGTCTGGAGA TGGATGAAAG TCACAGACCA GTGAAACTGA GGAAAGTGGT GCTTGGCTTC 
CCTTGTGGAA GTAATCAAAA TGAATTCAAG TTTGATCTAT CTCTCAACTA CAAACTTGCC 
AACATTATTC AGACCTACTC TGACCAAAAA CCAACCCTTG TTTTTTGCTC CACAAGAAAA 
GGAGTTCAAC AATCTGCTTC TGTGCTTGCC AAGGATGCCA GGTTTATCAT GAGCATTGAC 
CACAAACAAA GACTGGTGAA ATATGCAAAC TCTCTGCTTG ATGCAAAACT CAGAGATCTA 
TTTGTTTATG GCATTGGATA TCATCACGCT GGCATGGATG TGTCCGACAG GAAGATCACC 
GAAAACGCAT TTACAGTGGG TGACTTGCCA GTTTTATTTA CAACAAGTAC TCTAGCCATG 
GGTGTGAATA TGCCAGCCCA CCTTGTGGTA ATCAAATCCA CAATGCACTA TGTTGGTGGT 
GCATGTGAGG AGTACAGTGA AGCTGACCTG TTACAAATGA TTGGAAGAGC TGGCAGACCA 
CAGTTTGACA CAACAGCAAC TGCAGTAATC ATGACACGGC CTCAGACAAA GGACAAATAC 
ATGCATTTCT TGAGTGGCTT AGACTGCATA GAGAGCAGTT TGCATACAAA TCTGGTGGAG 
CACTTGAATG CAGAAATAGT TCTTCACACC ATATCTGATG TGAACATGGC TCTGGACTGG 
ATCCGCTCTA CTTTCCTGTA CATCCGTGCT TTGAAAAACC CCAAACACTA TGGCTTTCCC 
CCTGAGGTTG ACAAATGTGG AATTGAGACT AAATTACAAG AACTGTGCTT GAAGAACCTC 
AACTCTTTGG CCTCTTTCAA CCTGATTACC ATGGATGAAG ACATTAACAT CAAGCCCACA 
GAGACTGGAA AACTCATGGC TAGGTACTGT GTTGCCTTTG ACACTGTGAA GCAGTTCAGC 
ATGGTGACTG GCACAGAGAC CTTACCTGAG CTGATTGAAA TGATATCGAA AGCAAAAGAG 
TTCAGTGACA TGCAATTAAG AGTAAATGAG AGAAAAACCT TGAACACATT GAATAAGGAT 
AAAAACCGAA CCACCATCAG GTTTCCAATA GAAGGGAAGA TCAAAAGCAA CGACATGAAA 
GTGAATTGTC TGATACAAGC TCAGCTTGGC TGCATCCCCA TTCAGGAGTT TGGACTCATA 
CAGGATACTG GAAAAATATT CAGAAATGCA GTTAGAGTTA GCAGATATCT GACTGAGTTT 
CTCTGTCATC ACTCCAAATC GAACTTTGCA GCTCAACTCA ACGCTTTAAT TCTTGCCAAA 
TGTTTTCGAG CCAAACTGTG GGAAAACTCA CCCTACATTT CCAAACAGCT TGAGAGGATA 
GGATTATCAC TTGCTACTGC GTTGGTGAAT GCAGGCCTTA CGACATTCAG CAAAATTGAG 
CAGACCAGTC CTAGAGAATT GGAGCTAATT GTCAACAGGC ACCCTCCATT TGGAAACCAG 
ATTAAGGAAG CCATCAGCAA ACTTCCCAGA TGTGAAGTTA GTTTGGAACA GATTCCACGA 
TACAGTCCAT CCACAGCTGA GATTGTTTTC ACAGTCAACA TGAATAACTT TAAGGAGTTG 
GCACTGAAGC GGACAGCGCC AGACCACCAT TATGTCACTC TTATTGTTGG AGATTGTGAC 
AATAAGGTGG TCTTCCAACA AAAAATCTCG GACACTTTGC TCTTGAAGTC TGGGAGCTGG 
TCAAAGAGAA TTGAGGTGAC AAGATCACCA AAGGGAGAGG AGCTCAGCAT TCATTTGATA 
AGCTCTGAAT ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HFM1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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