Dcaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcaf1
Entrez Gene ID	321006
Full Name	DDB1 and CUL4 associated factor 1
Synonyms	AI447437,B930007L02Rik,Vprbp,mKIAA0800
General protein information	Preferred Names DDB1 and CUL4 associated factor 1 Names DDB1- and CUL4-associated factor 1 protein VPRBP Vpr (HIV-1) binding protein serine/threonine-protein kinase VPRBP
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511755.3	XP_006511818.1	protein VPRBP isoform X3
NM_001373930.1	NP_001360859.1	DDB1- and CUL4-associated factor 1 isoform 1
XM_006511754.3	XP_006511817.1	DDB1- and CUL4-associated factor 1 isoform X2
NM_001015507.3	NP_001015507.1	DDB1- and CUL4-associated factor 1 isoform 3
NM_001373931.1	NP_001360860.1	DDB1- and CUL4-associated factor 1 isoform 2
NM_001015507.2	NP_001015507.1	DDB1- and CUL4-associated factor 1
XM_006511753.3	XP_006511816.1	DDB1- and CUL4-associated factor 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	VPRBP	XP_005632545.1
Drosophila melanogaster (fruit fly)	mahj	NP_001246451.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012134	XP_320393.4
Mus musculus (house mouse)	Vprbp	NP_001015507.1
Rattus norvegicus (Norway rat)	Vprbp	NP_001178727.1
Gallus gallus (chicken)	VPRBP	XP_004944460.1
Xenopus tropicalis (tropical clawed frog)	vprbp	XP_004914132.1
Bos taurus (cattle)	VPRBP	XP_005223061.1
Caenorhabditis elegans (roundworm)	dcaf-1	NP_501725.2
Homo sapiens (human)	VPRBP	NP_055518.1
Pan troglodytes (chimpanzee)	VPRBP	XP_001169544.2

Gene Ontology
Bibliography

Related articles in PubMed

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.
Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, Whitmire JK, Xiong Y, Chen X, Wan YY
Nature communications710307(2016 Jan)

VprBP Is Required for Efficient Editing and Selection of Ig?+ B Cells, but Is Dispensable for Ig?+ and Marginal Zone B Cell Maturation and Selection.
Palmer VL, Aziz-Seible R, Kassmeier MD, Rothermund M, Perry GA, Swanson PC
Journal of immunology (Baltimore, Md. : 1950)195(4)1524-37(2015 Aug)

CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.
Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, Fan HY, Chen X, Xiong Y
Molecular cell57(2)247-260(2015 Jan)

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Yu C, Zhang YL, Pan WW, Li XM, Wang ZW, Ge ZJ, Zhou JJ, Cang Y, Tong C, Sun QY, Fan HY
Science (New York, N.Y.)342(6165)1518-21(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DCAF1 is required for T-cell expansion and function during anti-viral and autoimmune responses.
Title: DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.

VprBP Is Required for Efficient Editing and Selection of Igkappa+ B Cells, but Is Dispensable for Iglambda+ and Marginal Zone B Cell Maturation and Selection
Title: VprBP Is Required for Efficient Editing and Selection of Ig?+ B Cells, but Is Dispensable for Ig?+ and Marginal Zone B Cell Maturation and Selection.

CRL4(VPRBP) ubiquitin ligase is a guardian of female reproductive life in germ cells and a maternal reprogramming factor after fertilization.
Title: CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

multi-subunit cullin RING E3 ligase complex VprBP/DDB1/Cul4A/Roc1 associates with full-length RAG1 through VprBP
Title: VprBP binds full-length RAG1 and is required for B-cell development and V(D)J recombination fidelity.

Data show that human immunodeficiency virus type 1 Vpr-binding protein VprBP binds stoichiometrically with DDB1 through its WD40 domain and through DDB1 to CUL4A, subunits of the COP9/signalsome.
Title: Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development.

The following Dcaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23227	NM_001015507.3 Latest version!	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu90555	NM_001373931.1 Latest version!	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu43184	NM_001373930.1 Latest version!	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu43182	XM_006511753.3 Latest version!	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43183	XM_006511754.3 Latest version!	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43184	XM_006511755.3 Latest version!	Mus musculus Vpr (HIV-1) binding protein (Vprbp), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu23227	NM_001015507.2	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$832.30 $1189.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcaf1 ( NM_001015507.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001015507.1 Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu23227
Clone ID Related Accession (Same CDS sequence)	NM_001015507.3 , NM_001015507.2
Accession Version	NM_001015507.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-04
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147636.3. On Sep 5, 2019 this sequence version replaced NM_001015507.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGC AGACAGCTCG GACAACTCTG ATTTGGAAGA TGACATCATC TTATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015507.1
CDS	177..4436
Translation	MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQQTARTTLIWKMTSSYL

Target ORF information:

RefSeq Version	NM_001015507.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015507.3

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGC AGACAGCTCG GACAACTCTG ATTTGGAAGA TGACATCATC TTATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf1 ( NM_001015507.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001015507.1 Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), mRNA.

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CloneID	OMu23227
Clone ID Related Accession (Same CDS sequence)	NM_001015507.3 , NM_001015507.2
Accession Version	NM_001015507.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-22
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC065119.1 and AC147636.3. On Sep 22, 2009 this sequence version replaced NM_001015507.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGC AGACAGCTCG GACAACTCTG ATTTGGAAGA TGACATCATC TTATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015507.1
CDS	125..4384
Translation	MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQQTARTTLIWKMTSSYL

Target ORF information:

RefSeq Version	NM_001015507.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015507.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGC AGACAGCTCG GACAACTCTG ATTTGGAAGA TGACATCATC TTATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf1 ( NM_001373931.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001360860.1 Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu90555
Clone ID Related Accession (Same CDS sequence)	NM_001373931.1
Accession Version	NM_001373931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-04
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147636.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.2587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGGTTTG CATGCATCCT 
CACAATGTTC TATCTGATGT GGTGAACTAT ACCCTGTGGT TAATGGAATG TTCTCATGCT 
TCAGGATGTT GCCATGCTAC TATGTTTTTC TCAATTTGTT TCTCATTCCG GGCTGTTTTG 
GAGCTCTTTG ACCGTTATGA TGGTCTTCGT CGTCTGGTGA ACTTGATCAG TACTTTGGAG 
ATTTTAAATT TGGAAGACCA GGGTGCACTT TTGAGTGATG ATGAAATATT TGCTAGTCGC 
CAAACAGGAA AACATACCTG CATGGCTCTG CGCAAGTATT TTGAGGCTCA TCTGGCCATT 
AAATTGGAAC AAGTCAAGCA GTCACTTCAG AGGACTGAGG GTGGTATTCT TGTTCACCCC 
CAGCCTCCAT ACAAGGCATG CTCATATACT CATGAGCAGA TTGTAGAAAT GATGGAATTT 
TTGATAGAGT ATGGCCCAGC TCAGCTATAT TGGGAACCTG CTGAAGTCTT CCTCAAGCTT 
TCCTGTGTGC AGCTTTTACT ACAGCTTATT TCCATCGCCT GCAATTGGAA GACCTATTAT 
GCAAGGAATG ATACTGTGCG TTTTGCTTTG GATGTCCTTG CTATTCTCAC TGTGGTGCCA 
AAAATCCAGC TCCAGTTGGC GGAGTCAGTG GATGTACTGG ACGAGGCCGG CTCTGCTGTC 
TCCACTGTAG GTATCAGCAT TATTTTGGGC GTGGCTGAGG GTGAGTTCTT TATCCATGAT 
GCTGAAATTC AGAAGTCAGC ACTTCAAATT ATCATCAATT GTGTATGTGG TCCAGATAAC 
CGAATATCCA GTATTGGCAA ATTTATTTCT GGAACTCCTC GGAGAAAGCT GTCTCAGACT 
CCCAAGAGTA GTGAGCATAC TCTGGCCAAG ATGTGGAATG TGGTCCAGTC CAACAATGGC 
ATCAAGGTGC TTCTGTCCCT GTTGTCCATC AAGATGCCCA TTACAGACGC AGACCAGATC 
CGGGCCCTGG CCTGCAAAGC CCTTGTAGGC CTGTCTCGAA GTAGCACTGT TCGACAGATC 
ATCAGTAAAC TTCCCCTTTT TAGTAGCTGC CAGATTCAAC AGCTGATGAA AGAGCCTGTG 
CTGCAGGATA AGCGTAGTGA CCATGTCAAG TTCTGCAAAT ACGCTGCTGA GCTCATTGAG 
CGTGTATCAG GAAAGCCTCT TCTCATTGGC ACTGATGTGT CTCTCGCACG ACTGCAGAAA 
GCAGATGTTG TTGCTCAGTC GAGGATCTCC TTCCCTGAGA AAGAGCTGCT CCTGTTGATA 
CGGAACCATC TCATTTCTAA AGGGCTTGGA GAGACAGCAA CTGTGCTGAC GAGAGAGGCT 
GACCTGCCCA TGACTGCTGC CTCACATTCT TCTGCCTTCA CCCCAGTGAC TGCTGCTGCT 
TCTCCTGTCT CTCTTCCCCG GACCCCTCGG ATTGCCAACG GCATTGCAAG TCGACTGGGC 
AGCCATGCTA CTGTGGGTGC TTCTGCCCCT TCTGCTCCCC CTGCCCATCC TCCACCACGG 
CCCCCCCAGG GTTCATTGCC TTTGCCTGGC CCATCATATG CAGGCAATTC CCCTTTGATT 
GGTAGGATCA GTTTTATCAG AGAGAGGCCA TCACCGTGCA ATGGCAGGAA AATTCGAGTG 
TTGCGGCAGA AGTCGGACCA TGGTGCATAC AGCCAGAGCC CTGCCATAAA AAAACAATTG 
GATAGACATC TTCCTTCCCC ACCTACGCTG GACAGTATCA TCACAGAGTA TCTTAGAGAG 
CAACATGCTC GATGCAAGAA CCCCGTTGCC ACCTGTCCTC CTTTTTCTCT CTTTACTCCT 
CATCAGTGTC CTGAACCAAA ACAGAGGCGG CAAGCGCCAA TAAACTTTAC CTCAAGGCTA 
AACCGCAGGG CATCATTTCC AAAATATGGA GGGGTAGATG GCGGATGCTT TGATAGGCAC 
CTTATCTTTA GCAGATTTCG TCCGATTTCA GTATTCCGGG AAGCTAATGA AGATGAAAGT 
GGCTTCACCT GTTGTGCATT CTCAGCCCGG GAGCGGTTCC TGATGCTTGG CACTTGTACA 
GGGCAGCTGA AGCTCTACAA TGTGTTTAGT GGACAGGAAG AGGCCAGCTA TAACTGTCAC 
AACTCAGCCA TCACACACCT TGAACCGTCA AGGGATGGGT CCTTACTTCT GACATCTGCC 
ACTTGGAGCC AGCCTTTGTC TGCACTTTGG GGAATGAAAT CAGTATTTGA TATGAAGCAT 
TCCTTCACAG AAGATCACTA TGTTGAGTTC AGTAAGCACT CTCAGGATCG GGTCATAGGC 
ACAAAAGGAG ACATTGCCCA TATTTATGAT ATTCAGACTG GCAACAAGCT GTTGACTCTG 
TTTAACCCAG ATCTTGCCAA CAACTACAAG AGGAACTGTG CCACCTTTAA TCCTACAGAT 
GATCTTGTCT TAAATGATGG CGTCCTCTGG GATGTCCGCT CTGCACAGGC CATCCACAAG 
TTTGACAAGT TCAACATGAA TATCAGTGGT GTTTTCCATC CAAATGGGCT GGAAGTCATC 
ATCAATACAG AGATTTGGGA CCTTCGGACT TTCCATCTTT TACACACTGT TCCTGCTCTG 
GATCAATGCC GTGTTGTTTT TAACCACACT GGGACAGTGA TGTATGGAGC AATGTTGCAG 
GCAGATGATG AAGATGACTT ACTGGAAGAG AGGATGAAAA GCCCTTTTGG GTCATCCTTT 
CGAACATTCA ACGCAACTGA TTATAAACCT ATAGCAACTA TTGATGTGAA ACGAAACATC 
TTTGACCTGT GCACAGACAC CAAAGACTGT TATCTTGCTG TCATTGAGAA TCAAGGCAGC 
ATGGATGCAC TGAATATGGA CACAGTGTGC AGGCTCTATG AAGTGGGCAG GCAGCGTCTG 
GCAGAGGATG AGGATGAAGA GGAGGACCAG GAAGAGGAAG AACAGGAGGA AGAAGATGAT 
GATGAAGATG ATGATGATAC TGATGATTTA GATGAACTAG ACACTGACCA GTTGCTGGAG 
GCAGAACTGG AGGAGGATGA CAACAATGAG AATGCAGGAG AGGATGGAGA CAATGACTTC 
TCTCCCTCTG ATGAGGAGCT AGCAAACCTT CTAGAGGAGG GAGAGGAGGG GGAAGATGAA 
GACTCTGATG CAGATGAGGA GGTGGAGCTG ATCCTGGGGG ACACAGACAG CTCGGACAAC 
TCTGATTTGG AAGATGACAT CATCTTATCT CTGAATGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360860.1
CDS	177..4538
Translation	MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001373931.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373931.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGGTTTG CATGCATCCT 
CACAATGTTC TATCTGATGT GGTGAACTAT ACCCTGTGGT TAATGGAATG TTCTCATGCT 
TCAGGATGTT GCCATGCTAC TATGTTTTTC TCAATTTGTT TCTCATTCCG GGCTGTTTTG 
GAGCTCTTTG ACCGTTATGA TGGTCTTCGT CGTCTGGTGA ACTTGATCAG TACTTTGGAG 
ATTTTAAATT TGGAAGACCA GGGTGCACTT TTGAGTGATG ATGAAATATT TGCTAGTCGC 
CAAACAGGAA AACATACCTG CATGGCTCTG CGCAAGTATT TTGAGGCTCA TCTGGCCATT 
AAATTGGAAC AAGTCAAGCA GTCACTTCAG AGGACTGAGG GTGGTATTCT TGTTCACCCC 
CAGCCTCCAT ACAAGGCATG CTCATATACT CATGAGCAGA TTGTAGAAAT GATGGAATTT 
TTGATAGAGT ATGGCCCAGC TCAGCTATAT TGGGAACCTG CTGAAGTCTT CCTCAAGCTT 
TCCTGTGTGC AGCTTTTACT ACAGCTTATT TCCATCGCCT GCAATTGGAA GACCTATTAT 
GCAAGGAATG ATACTGTGCG TTTTGCTTTG GATGTCCTTG CTATTCTCAC TGTGGTGCCA 
AAAATCCAGC TCCAGTTGGC GGAGTCAGTG GATGTACTGG ACGAGGCCGG CTCTGCTGTC 
TCCACTGTAG GTATCAGCAT TATTTTGGGC GTGGCTGAGG GTGAGTTCTT TATCCATGAT 
GCTGAAATTC AGAAGTCAGC ACTTCAAATT ATCATCAATT GTGTATGTGG TCCAGATAAC 
CGAATATCCA GTATTGGCAA ATTTATTTCT GGAACTCCTC GGAGAAAGCT GTCTCAGACT 
CCCAAGAGTA GTGAGCATAC TCTGGCCAAG ATGTGGAATG TGGTCCAGTC CAACAATGGC 
ATCAAGGTGC TTCTGTCCCT GTTGTCCATC AAGATGCCCA TTACAGACGC AGACCAGATC 
CGGGCCCTGG CCTGCAAAGC CCTTGTAGGC CTGTCTCGAA GTAGCACTGT TCGACAGATC 
ATCAGTAAAC TTCCCCTTTT TAGTAGCTGC CAGATTCAAC AGCTGATGAA AGAGCCTGTG 
CTGCAGGATA AGCGTAGTGA CCATGTCAAG TTCTGCAAAT ACGCTGCTGA GCTCATTGAG 
CGTGTATCAG GAAAGCCTCT TCTCATTGGC ACTGATGTGT CTCTCGCACG ACTGCAGAAA 
GCAGATGTTG TTGCTCAGTC GAGGATCTCC TTCCCTGAGA AAGAGCTGCT CCTGTTGATA 
CGGAACCATC TCATTTCTAA AGGGCTTGGA GAGACAGCAA CTGTGCTGAC GAGAGAGGCT 
GACCTGCCCA TGACTGCTGC CTCACATTCT TCTGCCTTCA CCCCAGTGAC TGCTGCTGCT 
TCTCCTGTCT CTCTTCCCCG GACCCCTCGG ATTGCCAACG GCATTGCAAG TCGACTGGGC 
AGCCATGCTA CTGTGGGTGC TTCTGCCCCT TCTGCTCCCC CTGCCCATCC TCCACCACGG 
CCCCCCCAGG GTTCATTGCC TTTGCCTGGC CCATCATATG CAGGCAATTC CCCTTTGATT 
GGTAGGATCA GTTTTATCAG AGAGAGGCCA TCACCGTGCA ATGGCAGGAA AATTCGAGTG 
TTGCGGCAGA AGTCGGACCA TGGTGCATAC AGCCAGAGCC CTGCCATAAA AAAACAATTG 
GATAGACATC TTCCTTCCCC ACCTACGCTG GACAGTATCA TCACAGAGTA TCTTAGAGAG 
CAACATGCTC GATGCAAGAA CCCCGTTGCC ACCTGTCCTC CTTTTTCTCT CTTTACTCCT 
CATCAGTGTC CTGAACCAAA ACAGAGGCGG CAAGCGCCAA TAAACTTTAC CTCAAGGCTA 
AACCGCAGGG CATCATTTCC AAAATATGGA GGGGTAGATG GCGGATGCTT TGATAGGCAC 
CTTATCTTTA GCAGATTTCG TCCGATTTCA GTATTCCGGG AAGCTAATGA AGATGAAAGT 
GGCTTCACCT GTTGTGCATT CTCAGCCCGG GAGCGGTTCC TGATGCTTGG CACTTGTACA 
GGGCAGCTGA AGCTCTACAA TGTGTTTAGT GGACAGGAAG AGGCCAGCTA TAACTGTCAC 
AACTCAGCCA TCACACACCT TGAACCGTCA AGGGATGGGT CCTTACTTCT GACATCTGCC 
ACTTGGAGCC AGCCTTTGTC TGCACTTTGG GGAATGAAAT CAGTATTTGA TATGAAGCAT 
TCCTTCACAG AAGATCACTA TGTTGAGTTC AGTAAGCACT CTCAGGATCG GGTCATAGGC 
ACAAAAGGAG ACATTGCCCA TATTTATGAT ATTCAGACTG GCAACAAGCT GTTGACTCTG 
TTTAACCCAG ATCTTGCCAA CAACTACAAG AGGAACTGTG CCACCTTTAA TCCTACAGAT 
GATCTTGTCT TAAATGATGG CGTCCTCTGG GATGTCCGCT CTGCACAGGC CATCCACAAG 
TTTGACAAGT TCAACATGAA TATCAGTGGT GTTTTCCATC CAAATGGGCT GGAAGTCATC 
ATCAATACAG AGATTTGGGA CCTTCGGACT TTCCATCTTT TACACACTGT TCCTGCTCTG 
GATCAATGCC GTGTTGTTTT TAACCACACT GGGACAGTGA TGTATGGAGC AATGTTGCAG 
GCAGATGATG AAGATGACTT ACTGGAAGAG AGGATGAAAA GCCCTTTTGG GTCATCCTTT 
CGAACATTCA ACGCAACTGA TTATAAACCT ATAGCAACTA TTGATGTGAA ACGAAACATC 
TTTGACCTGT GCACAGACAC CAAAGACTGT TATCTTGCTG TCATTGAGAA TCAAGGCAGC 
ATGGATGCAC TGAATATGGA CACAGTGTGC AGGCTCTATG AAGTGGGCAG GCAGCGTCTG 
GCAGAGGATG AGGATGAAGA GGAGGACCAG GAAGAGGAAG AACAGGAGGA AGAAGATGAT 
GATGAAGATG ATGATGATAC TGATGATTTA GATGAACTAG ACACTGACCA GTTGCTGGAG 
GCAGAACTGG AGGAGGATGA CAACAATGAG AATGCAGGAG AGGATGGAGA CAATGACTTC 
TCTCCCTCTG ATGAGGAGCT AGCAAACCTT CTAGAGGAGG GAGAGGAGGG GGAAGATGAA 
GACTCTGATG CAGATGAGGA GGTGGAGCTG ATCCTGGGGG ACACAGACAG CTCGGACAAC 
TCTGATTTGG AAGATGACAT CATCTTATCT CTGAATGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf1 ( NM_001373930.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001360859.1 Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 1, mRNA.

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CloneID	OMu43184
Clone ID Related Accession (Same CDS sequence)	XM_006511755.3 , NM_001373930.1
Accession Version	NM_001373930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147636.3. On Sep 5, 2019 this sequence version replaced XM_006511755.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.16432.1, SRR6116558.101664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGG AAGAGGAAGA ACAGGAGGAA GAAGATGATG ATGAAGATGA TGATGATACT 
GATGATTTAG ATGAACTAGA CACTGACCAG TTGCTGGAGG CAGAACTGGA GGAGGATGAC 
AACAATGAGA ATGCAGGAGA GGATGGAGAC AATGACTTCT CTCCCTCTGA TGAGGAGCTA 
GCAAACCTTC TAGAGGAGGG AGAGGAGGGG GAAGATGAAG ACTCTGATGC AGATGAGGAG 
GTGGAGCTGA TCCTGGGGGA CACAGACAGC TCGGACAACT CTGATTTGGA AGATGACATC 
ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360859.1
CDS	177..4697
Translation	MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001373930.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373930.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGG AAGAGGAAGA ACAGGAGGAA GAAGATGATG ATGAAGATGA TGATGATACT 
GATGATTTAG ATGAACTAGA CACTGACCAG TTGCTGGAGG CAGAACTGGA GGAGGATGAC 
AACAATGAGA ATGCAGGAGA GGATGGAGAC AATGACTTCT CTCCCTCTGA TGAGGAGCTA 
GCAAACCTTC TAGAGGAGGG AGAGGAGGGG GAAGATGAAG ACTCTGATGC AGATGAGGAG 
GTGGAGCTGA TCCTGGGGGA CACAGACAGC TCGGACAACT CTGATTTGGA AGATGACATC 
ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf1 ( XM_006511753.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511816.1 Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant X1, mRNA.

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CloneID	OMu43182
Clone ID Related Accession (Same CDS sequence)	XM_006511753.3
Accession Version	XM_006511753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATA GCTGTCCATA CGGCCAAGTC ATGACTACAG TAGTGGTACA TGTGGACTCC 
AAAGCTGAGC TCACTACCCT GCTGGAGCAG TGGGAAAAGG ACCATGGCAG TGGCCAGGAT 
ATGGTACCAA TCCTTACCAG AATGTCTGAA TTAATTGAAA AAGAAACTGA AGAATATCGT 
AAAGGAGATC CAGATCCATT TGATGATCGA CATCCTGGTC GAGCTGATCC AGAGTGTATG 
CTGGGCCATT TGCTGAGAAT ACTCTTCAAG AATGATGATT TCATGAATGC GCTGGTAAAT 
GCATATGTGA TGACAAGTAG AGAGCCCCCT TTAAATACTG CAGCTTGCAG ACTGCTGCTG 
GACATCATGC CGGGCCTAGA AACCGCAGTT GTCTTTCAAG AAAAGGAAGG AATTGTTGAA 
AATCTTTTCA AATGGGCCCG AGAGGCTGAT CAGCCACTTA GGACATATTC TACTGGACTG 
CTGGGAGGTG CTATGGAAAA TCAAGATATT GCTGCCAACT ACAGAGATGA AAATTCACAG 
TTGGTGGCAA TAGTGCTTAG AAGACTGAGG GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG 
CAGGACAGTA AGCGTCCTAG TCCACGGAAG CTCTCTTCGG AACCCCTTTT GCCTCTGGAT 
GAGGAAGCTG TGGATATGGA CTATGGTGAC ATGGCAGTGG ATGTTGTGGA TGGAGAACAA 
GAATCTTCTA GAGATATGGA GATCTCCTTT CGTCTTGATT CAAGTCACAA GACCAGTAGT 
AGAGTGAACT CAGCAACTAA ACCTGAGGAA GGAGGATTAA AGAAAAACAA GTCAGCAAAG 
CATGGTGACA GAGAGAACTT TAGGAAGGCC AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC 
CCAGATCGTG TGTTTGTTGA GCTGTCTAAT AGCAGCTGGT CAGAAATGTC CCCCTGGGTG 
ATCGGCACCA ATTACACTCT CTATCCTATG ACTCCTGCTA TTGAGCAGAG ACTTATTCTT 
CAGTATTTGA CCCCTTTAGG AGAGTATCAG GAGTTACTTC CCATATTCAT GCAACTTGGA 
TCAAGAGAAT TGATGATGTT TTATATTGAT CTGAAACAGA CGAATGATGT CCTGCTTACT 
TTTGAGGCAC TCAAGCACCT AGCATCTCTC CTGCTTCATA ACAAATTTGC CACAGAATTT 
GTTGCACATG GTGGAGTACA GAAATTACTG GAAATTCCTC GTCCTTCTAT GGCTGCAACT 
GGTGTGTCCA TGTGCTTGTA TTACCTGTCC TACAATCAGG ATGCTATGGA AAGAGTTTGC 
ATGCATCCTC ACAATGTTCT ATCTGATGTG GTGAACTATA CCCTGTGGTT AATGGAATGT 
TCTCATGCTT CAGGATGTTG CCATGCTACT ATGTTTTTCT CAATTTGTTT CTCATTCCGG 
GCTGTTTTGG AGCTCTTTGA CCGTTATGAT GGTCTTCGTC GTCTGGTGAA CTTGATCAGT 
ACTTTGGAGA TTTTAAATTT GGAAGACCAG GGTGCACTTT TGAGTGATGA TGAAATATTT 
GCTAGTCGCC AAACAGGAAA ACATACCTGC ATGGCTCTGC GCAAGTATTT TGAGGCTCAT 
CTGGCCATTA AATTGGAACA AGTCAAGCAG TCACTTCAGA GGACTGAGGG TGGTATTCTT 
GTTCACCCCC AGCCTCCATA CAAGGCATGC TCATATACTC ATGAGCAGAT TGTAGAAATG 
ATGGAATTTT TGATAGAGTA TGGCCCAGCT CAGCTATATT GGGAACCTGC TGAAGTCTTC 
CTCAAGCTTT CCTGTGTGCA GCTTTTACTA CAGCTTATTT CCATCGCCTG CAATTGGAAG 
ACCTATTATG CAAGGAATGA TACTGTGCGT TTTGCTTTGG ATGTCCTTGC TATTCTCACT 
GTGGTGCCAA AAATCCAGCT CCAGTTGGCG GAGTCAGTGG ATGTACTGGA CGAGGCCGGC 
TCTGCTGTCT CCACTGTAGG TATCAGCATT ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT 
ATCCATGATG CTGAAATTCA GAAGTCAGCA CTTCAAATTA TCATCAATTG TGTATGTGGT 
CCAGATAACC GAATATCCAG TATTGGCAAA TTTATTTCTG GAACTCCTCG GAGAAAGCTG 
TCTCAGACTC CCAAGAGTAG TGAGCATACT CTGGCCAAGA TGTGGAATGT GGTCCAGTCC 
AACAATGGCA TCAAGGTGCT TCTGTCCCTG TTGTCCATCA AGATGCCCAT TACAGACGCA 
GACCAGATCC GGGCCCTGGC CTGCAAAGCC CTTGTAGGCC TGTCTCGAAG TAGCACTGTT 
CGACAGATCA TCAGTAAACT TCCCCTTTTT AGTAGCTGCC AGATTCAACA GCTGATGAAA 
GAGCCTGTGC TGCAGGATAA GCGTAGTGAC CATGTCAAGT TCTGCAAATA CGCTGCTGAG 
CTCATTGAGC GTGTATCAGG AAAGCCTCTT CTCATTGGCA CTGATGTGTC TCTCGCACGA 
CTGCAGAAAG CAGATGTTGT TGCTCAGTCG AGGATCTCCT TCCCTGAGAA AGAGCTGCTC 
CTGTTGATAC GGAACCATCT CATTTCTAAA GGGCTTGGAG AGACAGCAAC TGTGCTGACG 
AGAGAGGCTG ACCTGCCCAT GACTGCTGCC TCACATTCTT CTGCCTTCAC CCCAGTGACT 
GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG ACCCCTCGGA TTGCCAACGG CATTGCAAGT 
CGACTGGGCA GCCATGCTAC TGTGGGTGCT TCTGCCCCTT CTGCTCCCCC TGCCCATCCT 
CCACCACGGC CCCCCCAGGG TTCATTGCCT TTGCCTGGCC CATCATATGC AGGCAATTCC 
CCTTTGATTG GTAGGATCAG TTTTATCAGA GAGAGGCCAT CACCGTGCAA TGGCAGGAAA 
ATTCGAGTGT TGCGGCAGAA GTCGGACCAT GGTGCATACA GCCAGAGCCC TGCCATAAAA 
AAACAATTGG ATAGACATCT TCCTTCCCCA CCTACGCTGG ACAGTATCAT CACAGAGTAT 
CTTAGAGAGC AACATGCTCG ATGCAAGAAC CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC 
TTTACTCCTC ATCAGTGTCC TGAACCAAAA CAGAGGCGGC AAGCGCCAAT AAACTTTACC 
TCAAGGCTAA ACCGCAGGGC ATCATTTCCA AAATATGGAG GGGTAGATGG CGGATGCTTT 
GATAGGCACC TTATCTTTAG CAGATTTCGT CCGATTTCAG TATTCCGGGA AGCTAATGAA 
GATGAAAGTG GCTTCACCTG TTGTGCATTC TCAGCCCGGG AGCGGTTCCT GATGCTTGGC 
ACTTGTACAG GGCAGCTGAA GCTCTACAAT GTGTTTAGTG GACAGGAAGA GGCCAGCTAT 
AACTGTCACA ACTCAGCCAT CACACACCTT GAACCGTCAA GGGATGGGTC CTTACTTCTG 
ACATCTGCCA CTTGGAGCCA GCCTTTGTCT GCACTTTGGG GAATGAAATC AGTATTTGAT 
ATGAAGCATT CCTTCACAGA AGATCACTAT GTTGAGTTCA GTAAGCACTC TCAGGATCGG 
GTCATAGGCA CAAAAGGAGA CATTGCCCAT ATTTATGATA TTCAGACTGG CAACAAGCTG 
TTGACTCTGT TTAACCCAGA TCTTGCCAAC AACTACAAGA GGAACTGTGC CACCTTTAAT 
CCTACAGATG ATCTTGTCTT AAATGATGGC GTCCTCTGGG ATGTCCGCTC TGCACAGGCC 
ATCCACAAGT TTGACAAGTT CAACATGAAT ATCAGTGGTG TTTTCCATCC AAATGGGCTG 
GAAGTCATCA TCAATACAGA GATTTGGGAC CTTCGGACTT TCCATCTTTT ACACACTGTT 
CCTGCTCTGG ATCAATGCCG TGTTGTTTTT AACCACACTG GGACAGTGAT GTATGGAGCA 
ATGTTGCAGG CAGATGATGA AGATGACTTA CTGGAAGAGA GGATGAAAAG CCCTTTTGGG 
TCATCCTTTC GAACATTCAA CGCAACTGAT TATAAACCTA TAGCAACTAT TGATGTGAAA 
CGAAACATCT TTGACCTGTG CACAGACACC AAAGACTGTT ATCTTGCTGT CATTGAGAAT 
CAAGGCAGCA TGGATGCACT GAATATGGAC ACAGTGTGCA GGCTCTATGA AGTGGGCAGG 
CAGCGTCTGG CAGAGGATGA GGATGAAGAG GAGGACCAGG AAGAGGAAGA ACAGGAGGAA 
GAAGATGATG ATGAAGATGA TGATGATACT GATGATTTAG ATGAACTAGA CACTGACCAG 
TTGCTGGAGG CAGAACTGGA GGAGGATGAC AACAATGAGA ATGCAGGAGA GGATGGAGAC 
AATGACTTCT CTCCCTCTGA TGAGGAGCTA GCAAACCTTC TAGAGGAGGG AGAGGAGGGG 
GAAGATGAAG ACTCTGATGC AGATGAGGAG GTGGAGCTGA TCCTGGGGGA CACAGACAGC 
TCGGACAACT CTGATTTGGA AGATGACATC ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511816.1
CDS	31..4581
Translation	MKNSCPYGQVMTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_006511753.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511753.3

ORF Insert Sequence:

ATGAAGAATA GCTGTCCATA CGGCCAAGTC ATGACTACAG TAGTGGTACA TGTGGACTCC 
AAAGCTGAGC TCACTACCCT GCTGGAGCAG TGGGAAAAGG ACCATGGCAG TGGCCAGGAT 
ATGGTACCAA TCCTTACCAG AATGTCTGAA TTAATTGAAA AAGAAACTGA AGAATATCGT 
AAAGGAGATC CAGATCCATT TGATGATCGA CATCCTGGTC GAGCTGATCC AGAGTGTATG 
CTGGGCCATT TGCTGAGAAT ACTCTTCAAG AATGATGATT TCATGAATGC GCTGGTAAAT 
GCATATGTGA TGACAAGTAG AGAGCCCCCT TTAAATACTG CAGCTTGCAG ACTGCTGCTG 
GACATCATGC CGGGCCTAGA AACCGCAGTT GTCTTTCAAG AAAAGGAAGG AATTGTTGAA 
AATCTTTTCA AATGGGCCCG AGAGGCTGAT CAGCCACTTA GGACATATTC TACTGGACTG 
CTGGGAGGTG CTATGGAAAA TCAAGATATT GCTGCCAACT ACAGAGATGA AAATTCACAG 
TTGGTGGCAA TAGTGCTTAG AAGACTGAGG GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG 
CAGGACAGTA AGCGTCCTAG TCCACGGAAG CTCTCTTCGG AACCCCTTTT GCCTCTGGAT 
GAGGAAGCTG TGGATATGGA CTATGGTGAC ATGGCAGTGG ATGTTGTGGA TGGAGAACAA 
GAATCTTCTA GAGATATGGA GATCTCCTTT CGTCTTGATT CAAGTCACAA GACCAGTAGT 
AGAGTGAACT CAGCAACTAA ACCTGAGGAA GGAGGATTAA AGAAAAACAA GTCAGCAAAG 
CATGGTGACA GAGAGAACTT TAGGAAGGCC AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC 
CCAGATCGTG TGTTTGTTGA GCTGTCTAAT AGCAGCTGGT CAGAAATGTC CCCCTGGGTG 
ATCGGCACCA ATTACACTCT CTATCCTATG ACTCCTGCTA TTGAGCAGAG ACTTATTCTT 
CAGTATTTGA CCCCTTTAGG AGAGTATCAG GAGTTACTTC CCATATTCAT GCAACTTGGA 
TCAAGAGAAT TGATGATGTT TTATATTGAT CTGAAACAGA CGAATGATGT CCTGCTTACT 
TTTGAGGCAC TCAAGCACCT AGCATCTCTC CTGCTTCATA ACAAATTTGC CACAGAATTT 
GTTGCACATG GTGGAGTACA GAAATTACTG GAAATTCCTC GTCCTTCTAT GGCTGCAACT 
GGTGTGTCCA TGTGCTTGTA TTACCTGTCC TACAATCAGG ATGCTATGGA AAGAGTTTGC 
ATGCATCCTC ACAATGTTCT ATCTGATGTG GTGAACTATA CCCTGTGGTT AATGGAATGT 
TCTCATGCTT CAGGATGTTG CCATGCTACT ATGTTTTTCT CAATTTGTTT CTCATTCCGG 
GCTGTTTTGG AGCTCTTTGA CCGTTATGAT GGTCTTCGTC GTCTGGTGAA CTTGATCAGT 
ACTTTGGAGA TTTTAAATTT GGAAGACCAG GGTGCACTTT TGAGTGATGA TGAAATATTT 
GCTAGTCGCC AAACAGGAAA ACATACCTGC ATGGCTCTGC GCAAGTATTT TGAGGCTCAT 
CTGGCCATTA AATTGGAACA AGTCAAGCAG TCACTTCAGA GGACTGAGGG TGGTATTCTT 
GTTCACCCCC AGCCTCCATA CAAGGCATGC TCATATACTC ATGAGCAGAT TGTAGAAATG 
ATGGAATTTT TGATAGAGTA TGGCCCAGCT CAGCTATATT GGGAACCTGC TGAAGTCTTC 
CTCAAGCTTT CCTGTGTGCA GCTTTTACTA CAGCTTATTT CCATCGCCTG CAATTGGAAG 
ACCTATTATG CAAGGAATGA TACTGTGCGT TTTGCTTTGG ATGTCCTTGC TATTCTCACT 
GTGGTGCCAA AAATCCAGCT CCAGTTGGCG GAGTCAGTGG ATGTACTGGA CGAGGCCGGC 
TCTGCTGTCT CCACTGTAGG TATCAGCATT ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT 
ATCCATGATG CTGAAATTCA GAAGTCAGCA CTTCAAATTA TCATCAATTG TGTATGTGGT 
CCAGATAACC GAATATCCAG TATTGGCAAA TTTATTTCTG GAACTCCTCG GAGAAAGCTG 
TCTCAGACTC CCAAGAGTAG TGAGCATACT CTGGCCAAGA TGTGGAATGT GGTCCAGTCC 
AACAATGGCA TCAAGGTGCT TCTGTCCCTG TTGTCCATCA AGATGCCCAT TACAGACGCA 
GACCAGATCC GGGCCCTGGC CTGCAAAGCC CTTGTAGGCC TGTCTCGAAG TAGCACTGTT 
CGACAGATCA TCAGTAAACT TCCCCTTTTT AGTAGCTGCC AGATTCAACA GCTGATGAAA 
GAGCCTGTGC TGCAGGATAA GCGTAGTGAC CATGTCAAGT TCTGCAAATA CGCTGCTGAG 
CTCATTGAGC GTGTATCAGG AAAGCCTCTT CTCATTGGCA CTGATGTGTC TCTCGCACGA 
CTGCAGAAAG CAGATGTTGT TGCTCAGTCG AGGATCTCCT TCCCTGAGAA AGAGCTGCTC 
CTGTTGATAC GGAACCATCT CATTTCTAAA GGGCTTGGAG AGACAGCAAC TGTGCTGACG 
AGAGAGGCTG ACCTGCCCAT GACTGCTGCC TCACATTCTT CTGCCTTCAC CCCAGTGACT 
GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG ACCCCTCGGA TTGCCAACGG CATTGCAAGT 
CGACTGGGCA GCCATGCTAC TGTGGGTGCT TCTGCCCCTT CTGCTCCCCC TGCCCATCCT 
CCACCACGGC CCCCCCAGGG TTCATTGCCT TTGCCTGGCC CATCATATGC AGGCAATTCC 
CCTTTGATTG GTAGGATCAG TTTTATCAGA GAGAGGCCAT CACCGTGCAA TGGCAGGAAA 
ATTCGAGTGT TGCGGCAGAA GTCGGACCAT GGTGCATACA GCCAGAGCCC TGCCATAAAA 
AAACAATTGG ATAGACATCT TCCTTCCCCA CCTACGCTGG ACAGTATCAT CACAGAGTAT 
CTTAGAGAGC AACATGCTCG ATGCAAGAAC CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC 
TTTACTCCTC ATCAGTGTCC TGAACCAAAA CAGAGGCGGC AAGCGCCAAT AAACTTTACC 
TCAAGGCTAA ACCGCAGGGC ATCATTTCCA AAATATGGAG GGGTAGATGG CGGATGCTTT 
GATAGGCACC TTATCTTTAG CAGATTTCGT CCGATTTCAG TATTCCGGGA AGCTAATGAA 
GATGAAAGTG GCTTCACCTG TTGTGCATTC TCAGCCCGGG AGCGGTTCCT GATGCTTGGC 
ACTTGTACAG GGCAGCTGAA GCTCTACAAT GTGTTTAGTG GACAGGAAGA GGCCAGCTAT 
AACTGTCACA ACTCAGCCAT CACACACCTT GAACCGTCAA GGGATGGGTC CTTACTTCTG 
ACATCTGCCA CTTGGAGCCA GCCTTTGTCT GCACTTTGGG GAATGAAATC AGTATTTGAT 
ATGAAGCATT CCTTCACAGA AGATCACTAT GTTGAGTTCA GTAAGCACTC TCAGGATCGG 
GTCATAGGCA CAAAAGGAGA CATTGCCCAT ATTTATGATA TTCAGACTGG CAACAAGCTG 
TTGACTCTGT TTAACCCAGA TCTTGCCAAC AACTACAAGA GGAACTGTGC CACCTTTAAT 
CCTACAGATG ATCTTGTCTT AAATGATGGC GTCCTCTGGG ATGTCCGCTC TGCACAGGCC 
ATCCACAAGT TTGACAAGTT CAACATGAAT ATCAGTGGTG TTTTCCATCC AAATGGGCTG 
GAAGTCATCA TCAATACAGA GATTTGGGAC CTTCGGACTT TCCATCTTTT ACACACTGTT 
CCTGCTCTGG ATCAATGCCG TGTTGTTTTT AACCACACTG GGACAGTGAT GTATGGAGCA 
ATGTTGCAGG CAGATGATGA AGATGACTTA CTGGAAGAGA GGATGAAAAG CCCTTTTGGG 
TCATCCTTTC GAACATTCAA CGCAACTGAT TATAAACCTA TAGCAACTAT TGATGTGAAA 
CGAAACATCT TTGACCTGTG CACAGACACC AAAGACTGTT ATCTTGCTGT CATTGAGAAT 
CAAGGCAGCA TGGATGCACT GAATATGGAC ACAGTGTGCA GGCTCTATGA AGTGGGCAGG 
CAGCGTCTGG CAGAGGATGA GGATGAAGAG GAGGACCAGG AAGAGGAAGA ACAGGAGGAA 
GAAGATGATG ATGAAGATGA TGATGATACT GATGATTTAG ATGAACTAGA CACTGACCAG 
TTGCTGGAGG CAGAACTGGA GGAGGATGAC AACAATGAGA ATGCAGGAGA GGATGGAGAC 
AATGACTTCT CTCCCTCTGA TGAGGAGCTA GCAAACCTTC TAGAGGAGGG AGAGGAGGGG 
GAAGATGAAG ACTCTGATGC AGATGAGGAG GTGGAGCTGA TCCTGGGGGA CACAGACAGC 
TCGGACAACT CTGATTTGGA AGATGACATC ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf1 ( XM_006511754.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511817.1 Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant X2, mRNA.

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CloneID	OMu43183
Clone ID Related Accession (Same CDS sequence)	XM_006511754.3
Accession Version	XM_006511754.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DDB1- and CUL4-associated factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATA GCTGTCCATA CGGCCAAGTC ATGACTACAG TAGTGGTACA TGTGGACTCC 
AAAGCTGAGC TCACTACCCT GCTGGAGCAG TGGGAAAAGG ACCATGGCAG TGGCCAGGAT 
ATGGTACCAA TCCTTACCAG AATGTCTGAA TTAATTGAAA AAGAAACTGA AGAATATCGT 
AAAGGAGATC CAGATCCATT TGATGATCGA CATCCTGGTC GAGCTGATCC AGAGTGTATG 
CTGGGCCATT TGCTGAGAAT ACTCTTCAAG AATGATGATT TCATGAATGC GCTGGTAAAT 
GCATATGTGA TGACAAGTAG AGAGCCCCCT TTAAATACTG CAGCTTGCAG ACTGCTGCTG 
GACATCATGC CGGGCCTAGA AACCGCAGTT GTCTTTCAAG AAAAGGAAGG AATTGTTGAA 
AATCTTTTCA AATGGGCCCG AGAGGCTGAT CAGCCACTTA GGACATATTC TACTGGACTG 
CTGGGAGGTG CTATGGAAAA TCAAGATATT GCTGCCAACT ACAGAGATGA AAATTCACAG 
TTGGTGGCAA TAGTGCTTAG AAGACTGAGG GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG 
CAGGACAGTA AGCGTCCTAG TCCACGGAAG CTCTCTTCGG AACCCCTTTT GCCTCTGGAT 
GAGGAAGCTG TGGATATGGA CTATGGTGAC ATGGCAGTGG ATGTTGTGGA TGGAGAACAA 
GAATCTTCTA GAGATATGGA GATCTCCTTT CGTCTTGATT CAAGTCACAA GACCAGTAGT 
AGAGTGAACT CAGCAACTAA ACCTGAGGAA GGAGGATTAA AGAAAAACAA GTCAGCAAAG 
CATGGTGACA GAGAGAACTT TAGGAAGGCC AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC 
CCAGATCGTG TGTTTGTTGA GCTGTCTAAT AGCAGCTGGT CAGAAATGTC CCCCTGGGTG 
ATCGGCACCA ATTACACTCT CTATCCTATG ACTCCTGCTA TTGAGCAGAG ACTTATTCTT 
CAGTATTTGA CCCCTTTAGG AGAGTATCAG GAGTTACTTC CCATATTCAT GCAACTTGGA 
TCAAGAGAAT TGATGATGTT TTATATTGAT CTGAAACAGA CGAATGATGT CCTGCTTACT 
TTTGAGGCAC TCAAGCACCT AGCATCTCTC CTGCTTCATA ACAAATTTGC CACAGAATTT 
GTTGCACATG GTGGAGTACA GAAATTACTG GAAATTCCTC GTCCTTCTAT GGCTGCAACT 
GGTGTGTCCA TGTGCTTGTA TTACCTGTCC TACAATCAGG ATGCTATGGA AAGAGTTTGC 
ATGCATCCTC ACAATGTTCT ATCTGATGTG GTGAACTATA CCCTGTGGTT AATGGAATGT 
TCTCATGCTT CAGGATGTTG CCATGCTACT ATGTTTTTCT CAATTTGTTT CTCATTCCGG 
GCTGTTTTGG AGCTCTTTGA CCGTTATGAT GGTCTTCGTC GTCTGGTGAA CTTGATCAGT 
ACTTTGGAGA TTTTAAATTT GGAAGACCAG GGTGCACTTT TGAGTGATGA TGAAATATTT 
GCTAGTCGCC AAACAGGAAA ACATACCTGC ATGGCTCTGC GCAAGTATTT TGAGGCTCAT 
CTGGCCATTA AATTGGAACA AGTCAAGCAG TCACTTCAGA GGACTGAGGG TGGTATTCTT 
GTTCACCCCC AGCCTCCATA CAAGGCATGC TCATATACTC ATGAGCAGAT TGTAGAAATG 
ATGGAATTTT TGATAGAGTA TGGCCCAGCT CAGCTATATT GGGAACCTGC TGAAGTCTTC 
CTCAAGCTTT CCTGTGTGCA GCTTTTACTA CAGCTTATTT CCATCGCCTG CAATTGGAAG 
ACCTATTATG CAAGGAATGA TACTGTGCGT TTTGCTTTGG ATGTCCTTGC TATTCTCACT 
GTGGTGCCAA AAATCCAGCT CCAGTTGGCG GAGTCAGTGG ATGTACTGGA CGAGGCCGGC 
TCTGCTGTCT CCACTGTAGG TATCAGCATT ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT 
ATCCATGATG CTGAAATTCA GAAGTCAGCA CTTCAAATTA TCATCAATTG TGTATGTGGT 
CCAGATAACC GAATATCCAG TATTGGCAAA TTTATTTCTG GAACTCCTCG GAGAAAGCTG 
TCTCAGACTC CCAAGAGTAG TGAGCATACT CTGGCCAAGA TGTGGAATGT GGTCCAGTCC 
AACAATGGCA TCAAGGTGCT TCTGTCCCTG TTGTCCATCA AGATGCCCAT TACAGACGCA 
GACCAGATCC GGGCCCTGGC CTGCAAAGCC CTTGTAGGCC TGTCTCGAAG TAGCACTGTT 
CGACAGATCA TCAGTAAACT TCCCCTTTTT AGTAGCTGCC AGATTCAACA GCTGATGAAA 
GAGCCTGTGC TGCAGGATAA GCGTAGTGAC CATGTCAAGT TCTGCAAATA CGCTGCTGAG 
CTCATTGAGC GTGTATCAGG AAAGCCTCTT CTCATTGGCA CTGATGTGTC TCTCGCACGA 
CTGCAGAAAG CAGATGTTGT TGCTCAGTCG AGGATCTCCT TCCCTGAGAA AGAGCTGCTC 
CTGTTGATAC GGAACCATCT CATTTCTAAA GGGCTTGGAG AGACAGCAAC TGTGCTGACG 
AGAGAGGCTG ACCTGCCCAT GACTGCTGCC TCACATTCTT CTGCCTTCAC CCCAGTGACT 
GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG ACCCCTCGGA TTGCCAACGG CATTGCAAGT 
CGACTGGGCA GCCATGCTAC TGTGGGTGCT TCTGCCCCTT CTGCTCCCCC TGCCCATCCT 
CCACCACGGC CCCCCCAGGG TTCATTGCCT TTGCCTGGCC CATCATATGC AGGCAATTCC 
CCTTTGATTG GTAGGATCAG TTTTATCAGA GAGAGGCCAT CACCGTGCAA TGGCAGGAAA 
ATTCGAGTGT TGCGGCAGAA GTCGGACCAT GGTGCATACA GCCAGAGCCC TGCCATAAAA 
AAACAATTGG ATAGACATCT TCCTTCCCCA CCTACGCTGG ACAGTATCAT CACAGAGTAT 
CTTAGAGAGC AACATGCTCG ATGCAAGAAC CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC 
TTTACTCCTC ATCAGTGTCC TGAACCAAAA CAGAGGCGGC AAGCGCCAAT AAACTTTACC 
TCAAGGCTAA ACCGCAGGGC ATCATTTCCA AAATATGGAG GGGTAGATGG CGGATGCTTT 
GATAGGCACC TTATCTTTAG CAGATTTCGT CCGATTTCAG TATTCCGGGA AGCTAATGAA 
GATGAAAGTG GCTTCACCTG TTGTGCATTC TCAGCCCGGG AGCGGTTCCT GATGCTTGGC 
ACTTGTACAG GGCAGCTGAA GCTCTACAAT GTGTTTAGTG GACAGGAAGA GGCCAGCTAT 
AACTGTCACA ACTCAGCCAT CACACACCTT GAACCGTCAA GGGATGGGTC CTTACTTCTG 
ACATCTGCCA CTTGGAGCCA GCCTTTGTCT GCACTTTGGG GAATGAAATC AGTATTTGAT 
ATGAAGCATT CCTTCACAGA AGATCACTAT GTTGAGTTCA GTAAGCACTC TCAGGATCGG 
GTCATAGGCA CAAAAGGAGA CATTGCCCAT ATTTATGATA TTCAGACTGG CAACAAGCTG 
TTGACTCTGT TTAACCCAGA TCTTGCCAAC AACTACAAGA GGAACTGTGC CACCTTTAAT 
CCTACAGATG ATCTTGTCTT AAATGATGGC GTCCTCTGGG ATGTCCGCTC TGCACAGGCC 
ATCCACAAGT TTGACAAGTT CAACATGAAT ATCAGTGGTG TTTTCCATCC AAATGGGCTG 
GAAGTCATCA TCAATACAGA GATTTGGGAC CTTCGGACTT TCCATCTTTT ACACACTGTT 
CCTGCTCTGG ATCAATGCCG TGTTGTTTTT AACCACACTG GGACAGTGAT GTATGGAGCA 
ATGTTGCAGG CAGATGATGA AGATGACTTA CTGGAAGAGA GGATGAAAAG CCCTTTTGGG 
TCATCCTTTC GAACATTCAA CGCAACTGAT TATAAACCTA TAGCAACTAT TGATGTGAAA 
CGAAACATCT TTGACCTGTG CACAGACACC AAAGACTGTT ATCTTGCTGT CATTGAGAAT 
CAAGGCAGCA TGGATGCACT GAATATGGAC ACAGTGTGCA GGCTCTATGA AGTGGGCAGG 
CAGCGTCTGG CAGAGGATGA GGATGAAGAG GAGGACCAGG AAGAGGAAGA ACAGGAGGAA 
GAAGATGATG ATGAAGATGA TGATGATACT GATGATTTAG ATGAACTAGA CACTGACCAG 
TTGCTGGAGG CAGAACTGGA GGAGGATGAC AACAATGAGA ATGCAGGAGA GGATGGAGAC 
AATGACTTCT CTCCCTCTGA TGAGGAGCTA GCAAACCTTC TAGAGGAGGG AGAGGAGGGG 
GAAGATGAAG ACTCTGATGC AGATGAGGAG GTGGAGCTGA TCCTGGGGGA CAACAGCTCG 
GACAACTCTG ATTTGGAAGA TGACATCATC TTATCTCTGA ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511817.1
CDS	31..4578
Translation	MKNSCPYGQVMTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDNSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_006511754.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511754.3

ORF Insert Sequence:

ATGAAGAATA GCTGTCCATA CGGCCAAGTC ATGACTACAG TAGTGGTACA TGTGGACTCC 
AAAGCTGAGC TCACTACCCT GCTGGAGCAG TGGGAAAAGG ACCATGGCAG TGGCCAGGAT 
ATGGTACCAA TCCTTACCAG AATGTCTGAA TTAATTGAAA AAGAAACTGA AGAATATCGT 
AAAGGAGATC CAGATCCATT TGATGATCGA CATCCTGGTC GAGCTGATCC AGAGTGTATG 
CTGGGCCATT TGCTGAGAAT ACTCTTCAAG AATGATGATT TCATGAATGC GCTGGTAAAT 
GCATATGTGA TGACAAGTAG AGAGCCCCCT TTAAATACTG CAGCTTGCAG ACTGCTGCTG 
GACATCATGC CGGGCCTAGA AACCGCAGTT GTCTTTCAAG AAAAGGAAGG AATTGTTGAA 
AATCTTTTCA AATGGGCCCG AGAGGCTGAT CAGCCACTTA GGACATATTC TACTGGACTG 
CTGGGAGGTG CTATGGAAAA TCAAGATATT GCTGCCAACT ACAGAGATGA AAATTCACAG 
TTGGTGGCAA TAGTGCTTAG AAGACTGAGG GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG 
CAGGACAGTA AGCGTCCTAG TCCACGGAAG CTCTCTTCGG AACCCCTTTT GCCTCTGGAT 
GAGGAAGCTG TGGATATGGA CTATGGTGAC ATGGCAGTGG ATGTTGTGGA TGGAGAACAA 
GAATCTTCTA GAGATATGGA GATCTCCTTT CGTCTTGATT CAAGTCACAA GACCAGTAGT 
AGAGTGAACT CAGCAACTAA ACCTGAGGAA GGAGGATTAA AGAAAAACAA GTCAGCAAAG 
CATGGTGACA GAGAGAACTT TAGGAAGGCC AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC 
CCAGATCGTG TGTTTGTTGA GCTGTCTAAT AGCAGCTGGT CAGAAATGTC CCCCTGGGTG 
ATCGGCACCA ATTACACTCT CTATCCTATG ACTCCTGCTA TTGAGCAGAG ACTTATTCTT 
CAGTATTTGA CCCCTTTAGG AGAGTATCAG GAGTTACTTC CCATATTCAT GCAACTTGGA 
TCAAGAGAAT TGATGATGTT TTATATTGAT CTGAAACAGA CGAATGATGT CCTGCTTACT 
TTTGAGGCAC TCAAGCACCT AGCATCTCTC CTGCTTCATA ACAAATTTGC CACAGAATTT 
GTTGCACATG GTGGAGTACA GAAATTACTG GAAATTCCTC GTCCTTCTAT GGCTGCAACT 
GGTGTGTCCA TGTGCTTGTA TTACCTGTCC TACAATCAGG ATGCTATGGA AAGAGTTTGC 
ATGCATCCTC ACAATGTTCT ATCTGATGTG GTGAACTATA CCCTGTGGTT AATGGAATGT 
TCTCATGCTT CAGGATGTTG CCATGCTACT ATGTTTTTCT CAATTTGTTT CTCATTCCGG 
GCTGTTTTGG AGCTCTTTGA CCGTTATGAT GGTCTTCGTC GTCTGGTGAA CTTGATCAGT 
ACTTTGGAGA TTTTAAATTT GGAAGACCAG GGTGCACTTT TGAGTGATGA TGAAATATTT 
GCTAGTCGCC AAACAGGAAA ACATACCTGC ATGGCTCTGC GCAAGTATTT TGAGGCTCAT 
CTGGCCATTA AATTGGAACA AGTCAAGCAG TCACTTCAGA GGACTGAGGG TGGTATTCTT 
GTTCACCCCC AGCCTCCATA CAAGGCATGC TCATATACTC ATGAGCAGAT TGTAGAAATG 
ATGGAATTTT TGATAGAGTA TGGCCCAGCT CAGCTATATT GGGAACCTGC TGAAGTCTTC 
CTCAAGCTTT CCTGTGTGCA GCTTTTACTA CAGCTTATTT CCATCGCCTG CAATTGGAAG 
ACCTATTATG CAAGGAATGA TACTGTGCGT TTTGCTTTGG ATGTCCTTGC TATTCTCACT 
GTGGTGCCAA AAATCCAGCT CCAGTTGGCG GAGTCAGTGG ATGTACTGGA CGAGGCCGGC 
TCTGCTGTCT CCACTGTAGG TATCAGCATT ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT 
ATCCATGATG CTGAAATTCA GAAGTCAGCA CTTCAAATTA TCATCAATTG TGTATGTGGT 
CCAGATAACC GAATATCCAG TATTGGCAAA TTTATTTCTG GAACTCCTCG GAGAAAGCTG 
TCTCAGACTC CCAAGAGTAG TGAGCATACT CTGGCCAAGA TGTGGAATGT GGTCCAGTCC 
AACAATGGCA TCAAGGTGCT TCTGTCCCTG TTGTCCATCA AGATGCCCAT TACAGACGCA 
GACCAGATCC GGGCCCTGGC CTGCAAAGCC CTTGTAGGCC TGTCTCGAAG TAGCACTGTT 
CGACAGATCA TCAGTAAACT TCCCCTTTTT AGTAGCTGCC AGATTCAACA GCTGATGAAA 
GAGCCTGTGC TGCAGGATAA GCGTAGTGAC CATGTCAAGT TCTGCAAATA CGCTGCTGAG 
CTCATTGAGC GTGTATCAGG AAAGCCTCTT CTCATTGGCA CTGATGTGTC TCTCGCACGA 
CTGCAGAAAG CAGATGTTGT TGCTCAGTCG AGGATCTCCT TCCCTGAGAA AGAGCTGCTC 
CTGTTGATAC GGAACCATCT CATTTCTAAA GGGCTTGGAG AGACAGCAAC TGTGCTGACG 
AGAGAGGCTG ACCTGCCCAT GACTGCTGCC TCACATTCTT CTGCCTTCAC CCCAGTGACT 
GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG ACCCCTCGGA TTGCCAACGG CATTGCAAGT 
CGACTGGGCA GCCATGCTAC TGTGGGTGCT TCTGCCCCTT CTGCTCCCCC TGCCCATCCT 
CCACCACGGC CCCCCCAGGG TTCATTGCCT TTGCCTGGCC CATCATATGC AGGCAATTCC 
CCTTTGATTG GTAGGATCAG TTTTATCAGA GAGAGGCCAT CACCGTGCAA TGGCAGGAAA 
ATTCGAGTGT TGCGGCAGAA GTCGGACCAT GGTGCATACA GCCAGAGCCC TGCCATAAAA 
AAACAATTGG ATAGACATCT TCCTTCCCCA CCTACGCTGG ACAGTATCAT CACAGAGTAT 
CTTAGAGAGC AACATGCTCG ATGCAAGAAC CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC 
TTTACTCCTC ATCAGTGTCC TGAACCAAAA CAGAGGCGGC AAGCGCCAAT AAACTTTACC 
TCAAGGCTAA ACCGCAGGGC ATCATTTCCA AAATATGGAG GGGTAGATGG CGGATGCTTT 
GATAGGCACC TTATCTTTAG CAGATTTCGT CCGATTTCAG TATTCCGGGA AGCTAATGAA 
GATGAAAGTG GCTTCACCTG TTGTGCATTC TCAGCCCGGG AGCGGTTCCT GATGCTTGGC 
ACTTGTACAG GGCAGCTGAA GCTCTACAAT GTGTTTAGTG GACAGGAAGA GGCCAGCTAT 
AACTGTCACA ACTCAGCCAT CACACACCTT GAACCGTCAA GGGATGGGTC CTTACTTCTG 
ACATCTGCCA CTTGGAGCCA GCCTTTGTCT GCACTTTGGG GAATGAAATC AGTATTTGAT 
ATGAAGCATT CCTTCACAGA AGATCACTAT GTTGAGTTCA GTAAGCACTC TCAGGATCGG 
GTCATAGGCA CAAAAGGAGA CATTGCCCAT ATTTATGATA TTCAGACTGG CAACAAGCTG 
TTGACTCTGT TTAACCCAGA TCTTGCCAAC AACTACAAGA GGAACTGTGC CACCTTTAAT 
CCTACAGATG ATCTTGTCTT AAATGATGGC GTCCTCTGGG ATGTCCGCTC TGCACAGGCC 
ATCCACAAGT TTGACAAGTT CAACATGAAT ATCAGTGGTG TTTTCCATCC AAATGGGCTG 
GAAGTCATCA TCAATACAGA GATTTGGGAC CTTCGGACTT TCCATCTTTT ACACACTGTT 
CCTGCTCTGG ATCAATGCCG TGTTGTTTTT AACCACACTG GGACAGTGAT GTATGGAGCA 
ATGTTGCAGG CAGATGATGA AGATGACTTA CTGGAAGAGA GGATGAAAAG CCCTTTTGGG 
TCATCCTTTC GAACATTCAA CGCAACTGAT TATAAACCTA TAGCAACTAT TGATGTGAAA 
CGAAACATCT TTGACCTGTG CACAGACACC AAAGACTGTT ATCTTGCTGT CATTGAGAAT 
CAAGGCAGCA TGGATGCACT GAATATGGAC ACAGTGTGCA GGCTCTATGA AGTGGGCAGG 
CAGCGTCTGG CAGAGGATGA GGATGAAGAG GAGGACCAGG AAGAGGAAGA ACAGGAGGAA 
GAAGATGATG ATGAAGATGA TGATGATACT GATGATTTAG ATGAACTAGA CACTGACCAG 
TTGCTGGAGG CAGAACTGGA GGAGGATGAC AACAATGAGA ATGCAGGAGA GGATGGAGAC 
AATGACTTCT CTCCCTCTGA TGAGGAGCTA GCAAACCTTC TAGAGGAGGG AGAGGAGGGG 
GAAGATGAAG ACTCTGATGC AGATGAGGAG GTGGAGCTGA TCCTGGGGGA CAACAGCTCG 
GACAACTCTG ATTTGGAAGA TGACATCATC TTATCTCTGA ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcaf1 ( XM_006511755.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511818.1 Mus musculus Vpr (HIV-1) binding protein (Vprbp), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu43184
Clone ID Related Accession (Same CDS sequence)	XM_006511755.3 , NM_001373930.1
Accession Version	XM_006511755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein VPRBP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511755.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGG AAGAGGAAGA ACAGGAGGAA GAAGATGATG ATGAAGATGA TGATGATACT 
GATGATTTAG ATGAACTAGA CACTGACCAG TTGCTGGAGG CAGAACTGGA GGAGGATGAC 
AACAATGAGA ATGCAGGAGA GGATGGAGAC AATGACTTCT CTCCCTCTGA TGAGGAGCTA 
GCAAACCTTC TAGAGGAGGG AGAGGAGGGG GAAGATGAAG ACTCTGATGC AGATGAGGAG 
GTGGAGCTGA TCCTGGGGGA CACAGACAGC TCGGACAACT CTGATTTGGA AGATGACATC 
ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511818.1
CDS	83..4603
Translation	MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_006511755.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Vpr (HIV-1) binding protein (Vprbp), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511755.3

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG ACCATGGCAG TGGCCAGGAT ATGGTACCAA TCCTTACCAG AATGTCTGAA 
TTAATTGAAA AAGAAACTGA AGAATATCGT AAAGGAGATC CAGATCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC GCTGGTAAAT GCATATGTGA TGACAAGTAG AGAGCCCCCT 
TTAAATACTG CAGCTTGCAG ACTGCTGCTG GACATCATGC CGGGCCTAGA AACCGCAGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAA AATCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTTA GGACATATTC TACTGGACTG CTGGGAGGTG CTATGGAAAA TCAAGATATT 
GCTGCCAACT ACAGAGATGA AAATTCACAG TTGGTGGCAA TAGTGCTTAG AAGACTGAGG 
GAGCTCCAGC TTCAAGAAGT GGCTTTGCGG CAGGACAGTA AGCGTCCTAG TCCACGGAAG 
CTCTCTTCGG AACCCCTTTT GCCTCTGGAT GAGGAAGCTG TGGATATGGA CTATGGTGAC 
ATGGCAGTGG ATGTTGTGGA TGGAGAACAA GAATCTTCTA GAGATATGGA GATCTCCTTT 
CGTCTTGATT CAAGTCACAA GACCAGTAGT AGAGTGAACT CAGCAACTAA ACCTGAGGAA 
GGAGGATTAA AGAAAAACAA GTCAGCAAAG CATGGTGACA GAGAGAACTT TAGGAAGGCC 
AAGCAGAAGT TGGGCTTCTC ATCTTCTGAC CCAGATCGTG TGTTTGTTGA GCTGTCTAAT 
AGCAGCTGGT CAGAAATGTC CCCCTGGGTG ATCGGCACCA ATTACACTCT CTATCCTATG 
ACTCCTGCTA TTGAGCAGAG ACTTATTCTT CAGTATTTGA CCCCTTTAGG AGAGTATCAG 
GAGTTACTTC CCATATTCAT GCAACTTGGA TCAAGAGAAT TGATGATGTT TTATATTGAT 
CTGAAACAGA CGAATGATGT CCTGCTTACT TTTGAGGCAC TCAAGCACCT AGCATCTCTC 
CTGCTTCATA ACAAATTTGC CACAGAATTT GTTGCACATG GTGGAGTACA GAAATTACTG 
GAAATTCCTC GTCCTTCTAT GGCTGCAACT GGTGTGTCCA TGTGCTTGTA TTACCTGTCC 
TACAATCAGG ATGCTATGGA AAGAGTTTGC ATGCATCCTC ACAATGTTCT ATCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAATGT TCTCATGCTT CAGGATGTTG CCATGCTACT 
ATGTTTTTCT CAATTTGTTT CTCATTCCGG GCTGTTTTGG AGCTCTTTGA CCGTTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTTTAAATTT GGAAGACCAG 
GGTGCACTTT TGAGTGATGA TGAAATATTT GCTAGTCGCC AAACAGGAAA ACATACCTGC 
ATGGCTCTGC GCAAGTATTT TGAGGCTCAT CTGGCCATTA AATTGGAACA AGTCAAGCAG 
TCACTTCAGA GGACTGAGGG TGGTATTCTT GTTCACCCCC AGCCTCCATA CAAGGCATGC 
TCATATACTC ATGAGCAGAT TGTAGAAATG ATGGAATTTT TGATAGAGTA TGGCCCAGCT 
CAGCTATATT GGGAACCTGC TGAAGTCTTC CTCAAGCTTT CCTGTGTGCA GCTTTTACTA 
CAGCTTATTT CCATCGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA TACTGTGCGT 
TTTGCTTTGG ATGTCCTTGC TATTCTCACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCG 
GAGTCAGTGG ATGTACTGGA CGAGGCCGGC TCTGCTGTCT CCACTGTAGG TATCAGCATT 
ATTTTGGGCG TGGCTGAGGG TGAGTTCTTT ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAAATTA TCATCAATTG TGTATGTGGT CCAGATAACC GAATATCCAG TATTGGCAAA 
TTTATTTCTG GAACTCCTCG GAGAAAGCTG TCTCAGACTC CCAAGAGTAG TGAGCATACT 
CTGGCCAAGA TGTGGAATGT GGTCCAGTCC AACAATGGCA TCAAGGTGCT TCTGTCCCTG 
TTGTCCATCA AGATGCCCAT TACAGACGCA GACCAGATCC GGGCCCTGGC CTGCAAAGCC 
CTTGTAGGCC TGTCTCGAAG TAGCACTGTT CGACAGATCA TCAGTAAACT TCCCCTTTTT 
AGTAGCTGCC AGATTCAACA GCTGATGAAA GAGCCTGTGC TGCAGGATAA GCGTAGTGAC 
CATGTCAAGT TCTGCAAATA CGCTGCTGAG CTCATTGAGC GTGTATCAGG AAAGCCTCTT 
CTCATTGGCA CTGATGTGTC TCTCGCACGA CTGCAGAAAG CAGATGTTGT TGCTCAGTCG 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTC CTGTTGATAC GGAACCATCT CATTTCTAAA 
GGGCTTGGAG AGACAGCAAC TGTGCTGACG AGAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCACATTCTT CTGCCTTCAC CCCAGTGACT GCTGCTGCTT CTCCTGTCTC TCTTCCCCGG 
ACCCCTCGGA TTGCCAACGG CATTGCAAGT CGACTGGGCA GCCATGCTAC TGTGGGTGCT 
TCTGCCCCTT CTGCTCCCCC TGCCCATCCT CCACCACGGC CCCCCCAGGG TTCATTGCCT 
TTGCCTGGCC CATCATATGC AGGCAATTCC CCTTTGATTG GTAGGATCAG TTTTATCAGA 
GAGAGGCCAT CACCGTGCAA TGGCAGGAAA ATTCGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCATACA GCCAGAGCCC TGCCATAAAA AAACAATTGG ATAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATCAT CACAGAGTAT CTTAGAGAGC AACATGCTCG ATGCAAGAAC 
CCCGTTGCCA CCTGTCCTCC TTTTTCTCTC TTTACTCCTC ATCAGTGTCC TGAACCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACC TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAATATGGAG GGGTAGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTTCGT 
CCGATTTCAG TATTCCGGGA AGCTAATGAA GATGAAAGTG GCTTCACCTG TTGTGCATTC 
TCAGCCCGGG AGCGGTTCCT GATGCTTGGC ACTTGTACAG GGCAGCTGAA GCTCTACAAT 
GTGTTTAGTG GACAGGAAGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACACCTT 
GAACCGTCAA GGGATGGGTC CTTACTTCTG ACATCTGCCA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAATC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC TCAGGATCGG GTCATAGGCA CAAAAGGAGA CATTGCCCAT 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAACATGAAT 
ATCAGTGGTG TTTTCCATCC AAATGGGCTG GAAGTCATCA TCAATACAGA GATTTGGGAC 
CTTCGGACTT TCCATCTTTT ACACACTGTT CCTGCTCTGG ATCAATGCCG TGTTGTTTTT 
AACCACACTG GGACAGTGAT GTATGGAGCA ATGTTGCAGG CAGATGATGA AGATGACTTA 
CTGGAAGAGA GGATGAAAAG CCCTTTTGGG TCATCCTTTC GAACATTCAA CGCAACTGAT 
TATAAACCTA TAGCAACTAT TGATGTGAAA CGAAACATCT TTGACCTGTG CACAGACACC 
AAAGACTGTT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCACT GAATATGGAC 
ACAGTGTGCA GGCTCTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGG AAGAGGAAGA ACAGGAGGAA GAAGATGATG ATGAAGATGA TGATGATACT 
GATGATTTAG ATGAACTAGA CACTGACCAG TTGCTGGAGG CAGAACTGGA GGAGGATGAC 
AACAATGAGA ATGCAGGAGA GGATGGAGAC AATGACTTCT CTCCCTCTGA TGAGGAGCTA 
GCAAACCTTC TAGAGGAGGG AGAGGAGGGG GAAGATGAAG ACTCTGATGC AGATGAGGAG 
GTGGAGCTGA TCCTGGGGGA CACAGACAGC TCGGACAACT CTGATTTGGA AGATGACATC 
ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.
Nature communications710307(2016 Jan)
Guo Z,Kong Q,Liu C,Zhang S,Zou L,Yan F,Whitmire JK,Xiong Y,Chen X,Wan YY

VprBP Is Required for Efficient Editing and Selection of Ig?+ B Cells, but Is Dispensable for Ig?+ and Marginal Zone B Cell Maturation and Selection.
Journal of immunology (Baltimore, Md. : 1950)195(4)1524-37(2015 Aug)
Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.
Molecular cell57(2)247-260(2015 Jan)
Nakagawa T,Lv L,Nakagawa M,Yu Y,Yu C,D'Alessio AC,Nakayama K,Fan HY,Chen X,Xiong Y

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Science (New York, N.Y.)342(6165)1518-21(2013 Dec)
Yu C,Zhang YL,Pan WW,Li XM,Wang ZW,Ge ZJ,Zhou JJ,Cang Y,Tong C,Sun QY,Fan HY

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Dcaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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