DCAF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010825129.2	XP_010823431.1	protein VPRBP
XM_005196474.3	XP_005196531.1	protein VPRBP
XM_002697028.6	XP_002697074.2	DDB1- and CUL4-associated factor 1
XM_010817837.2	XP_010816139.1	protein VPRBP
XM_010817839.2	XP_010816141.1	protein VPRBP
XM_015459640.1	XP_015315126.1	protein VPRBP
XM_003583810.4	XP_003583858.1	protein VPRBP
XM_010825128.2	XP_010823430.1	protein VPRBP
XM_002697028.5	XP_002697074.2	protein VPRBP
XM_005223006.4	XP_005223063.1	DDB1- and CUL4-associated factor 1
XM_005223006.3	XP_005223063.1	protein VPRBP
XM_024983267.1	XP_024839035.1	DDB1- and CUL4-associated factor 1
XM_010825130.2	XP_010823432.1	protein VPRBP
XM_010817839.3	XP_010816141.1	DDB1- and CUL4-associated factor 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa11060	XM_010825128.2 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_024983267.1 Latest version!	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_005223006.4 Latest version!	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_015459640.1 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_010825130.2 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_010817837.2 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_003583810.4 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_010825129.2 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_005196474.3 Latest version!	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_002697028.6 Latest version!	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_010817839.3 Latest version!	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa11060	XM_002697028.5	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_005223006.3	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa11060	XM_010817839.2	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

DCAF1 ( XM_010825129.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823431.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_010825129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823431.1
CDS	70..4593
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_010825129.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825129.2

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005196474.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196531.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_005196474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196531.1
CDS	679..5202
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005196474.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196474.3

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_002697028.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697074.2 Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X1, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_002697028.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DDB1- and CUL4-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697028.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697074.2
CDS	154..4677
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_002697028.6
Organism	Bos taurus(cattle)
Definition	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697028.6

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_010817837.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816139.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_010817837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816139.1
CDS	1187..5710
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_010817837.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817837.2

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_010817839.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816141.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_010817839.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816141.1
CDS	208..4731
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_010817839.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817839.2

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_015459640.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315126.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_015459640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315126.1
CDS	70..4593
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_015459640.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459640.1

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_003583810.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583858.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_003583810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583858.1
CDS	156..4679
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_003583810.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583810.4

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_010825128.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823430.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_010825128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823430.1
CDS	1187..5710
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_010825128.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825128.2

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_002697028.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697074.2 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X1, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_002697028.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697028.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697074.2
CDS	156..4679
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_002697028.5
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697028.5

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005223006.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223063.1 Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_005223006.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DDB1- and CUL4-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223063.1
CDS	737..5260
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005223006.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223006.4

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005223006.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223063.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_005223006.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223063.1
CDS	679..5202
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005223006.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223006.3

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_024983267.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839035.1 Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X3, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_024983267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DDB1- and CUL4-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839035.1
CDS	1213..5736
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_024983267.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983267.1

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_010825130.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823432.1 Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_010825130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein VPRBP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823432.1
CDS	208..4731
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_010825130.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825130.2

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_010817839.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816141.1 Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X2, mRNA.

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CloneID	OBa11060
Clone ID Related Accession (Same CDS sequence)	XM_010825129.2 , XM_005196474.3 , XM_002697028.6 , XM_010817837.2 , XM_010817839.2 , XM_015459640.1 , XM_003583810.4 , XM_010825128.2 , XM_002697028.5 , XM_005223006.4 , XM_005223006.3 , XM_024983267.1 , XM_010825130.2 , XM_010817839.3
Accession Version	XM_010817839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DDB1- and CUL4-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816141.1
CDS	227..4750
Translation	MTTVAVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVGSRQESKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQEEASGDMEISFHLESGHKTSSRVNSATKLEEGALRKNKSTKQADRESFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSVKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGTSAPSAPAAHPQPRVPQGSLALPGPSHAGNSPVIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGLTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDAEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_010817839.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817839.3

ORF Insert Sequence:

ATGACTACAG TAGCGGTCCA TGTGGACTCC AAAGCTGAGC TCACTACCCT GTTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCCA TCCTTACCAG GATGTCTGAA 
TTGATTGAAA AAGAAACTGA AGAATATCGT AAGGGGGATC CAGACCCATT TGATGATCGG 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCATT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTACTG GACATCATGC CCGGGCTGGA AACTGCTGTT 
GTCTTTCAAG AAAAGGAAGG AATTGTTGAG AACCTTTTCA AATGGGCCCG AGAGGCTGAT 
CAGCCACTGA GGACATATTC TACTGGTCTG TTAGGTGGTG CTATGGAGAA TCAAGACATC 
GCTGCTAACT ACAGGGATGA GAACTCACAG CTGGTGGCAA TAGTGCTTCG AAGATTGAGG 
GAGCTGCAGC TTCAGGAAGT AGGTTCAAGG CAAGAAAGCA AGCGTCCTAG CCCACGAAAG 
CTCTCCTCTG AACCCCTTTT GCCTCTGGAT GAAGAAGCTG TGGATATGGA CTATGGCGAC 
ATGGCTGTAG ATGTAGTAGA TGGCGAACAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG AGTCAGGCCA CAAGACCAGT AGCAGAGTGA ACTCAGCAAC GAAACTTGAA 
GAAGGGGCAT TGAGGAAAAA CAAGTCAACA AAACAGGCTG ACAGAGAGAG CTTCAGGAAA 
GCCAAGCAAA AGTTGGGTTT TTCATCATCT GATCCAGACC GCATGTTTGT TGAATTGTCT 
AATAGCAGCT GGTCAGAAAT GTCCCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATTGAGCA GCGACTTATT CTCCAGTATT TGACCCCTTT AGGAGAATAT 
CAGGAGTTAC TTCCCATATT CATGCAGCTT GGATCACGGG AGTTGATGAT GTTCTACATT 
GACTTAAAAC AAACTAATGA TGTCCTGCTT ACATTTGAGG CGCTCAAGCA CCTAGCATCT 
CTCCTGCTCC ACAATAAGTT TGCCACAGAA TTTGTTGCGC ACGGTGGAGT ACAGAAATTA 
CTGGAAATTC CTCGTCCTTC TATGGCTGCA ACTGGTGTAT CCATGTGCTT GTATTACCTG 
TCCTACAACC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ACACCCTGTG GCTAATGGAA TGTTCACATG CCTCAGGATG CTGCCATGCT 
ACCATGTTTT TCTCAATCTG CTTCTCCTTC CGGGCTGTGT TGGAACTCTT TGACCGCTAC 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTCGCTAGCC GGCAGACTGG TAAACACACC 
TGCATGGCCT TGCGCAAGTA CTTTGAAGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCTCTTC AGAGGACTGA AGGTGGCATT CTTGTTCATC CTCAACCTCC CTACAAGGCA 
TGTTCATATA CTCATGAACA GATTGTGGAA ATGATGGAGT TTCTGATAGA ATATGGCCCA 
GCGCAGCTGT ATTGGGAGCC AGCTGAAGTC TTCCTCAAGC TGTCTTGTGT CCAGCTCTTA 
TTGCAACTTA TTTCTATTGC TTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTC TGGATGTCCT GGCTATTCTC ACTGTTGTGC CAAAAATTCA GCTCCAGTTG 
GCAGAATCAG TGGATGTACT AGATGAGGCT GGCTCCACCG TCTCCACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTTATTCACG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTTTGT GGCCCGGATA ACAGAATTTC TAGTATTGGC 
AAGTTTATCT CTGGAACGCC TCGGAGGAAG CTGCCTCAGA CGCCCAAAAG CAGTGAGCAC 
ACTCTGGCCA AGATGTGGAA CGTGGTGCAG TCCAACAACG GCATCAAGGT GCTTCTGTCC 
TTACTGTCTG TCAAGATGCC CATCACAGAT GCAGACCAGA TCCGGGCCCT AGCCTGCAAG 
GCCCTGGTGG GCCTGTCTCG CAGTAGCACC GTGCGGCAGA TCATCAGCAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAAGAGCCTG TGCTTCAGGA CAAGCGTAGT 
GACCACGTCA AGTTCTGCAA GTACGCCGCT GAGCTCATCG AACGGGTGTC GGGAAAGCCG 
CTTCTTATCG GCACCGATGT GTCCCTGGCA CGTCTGCAGA AAGCTGACGT TGTTGCCCAG 
TCACGGATCT CCTTTCCTGA GAAAGAGCTG CTTCTACTGA TCCGAAACCA TCTCATTTCT 
AAAGGCCTTG GGGAGACAGC CACTGTGCTG ACTAAAGAGG CCGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCGGCCTT CACCCCGGTC ACTGCTGCTG CTTCTCCTGT TTCTCTTCCC 
CGCACCCCTC GCATCGCCAA TGGCATTGCA ACTCGATTGG GTAGCCATGC TGCTGTGGGT 
ACCTCTGCCC CTTCTGCCCC CGCTGCTCAT CCTCAGCCGC GGGTGCCCCA GGGTTCACTA 
GCTCTGCCTG GTCCATCTCA TGCAGGAAAC TCCCCTGTGA TTGGTAGGAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGCGCCT ACAGCCAGAG CCCAGCCATA AAAAAGCAGC TGGACAGACA CCTTCCCTCC 
CCGCCTACGC TGGACAGTAT CATCACAGAA TATCTTAGAG AGCAGCATGC TCGCTGCAAG 
AACCCAGTTG CCACCTGCCC ACCCTTCTCT CTCTTTACTC CTCACCAGTG TCCTGAGCCA 
AAACAGAGGC GACAAGCGCC AATAAACTTT ACCTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTAGA TGGCGGGTGC TTTGATAGGC ACCTTATCTT TAGCAGGTTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAC GAGGATGAGA GTGGCCTCAC CTGCTGTGCG 
TTCTCCGCAC GAGAGCGCTT CCTGATGCTG GGCACCTGCA CCGGGCAGTT GAAGCTCTAC 
AACGTGTTCA GTGGACAGGA GGAGGCCAGC TACAACTGTC ACAACTCAGC CATCACACAC 
CTGGAGCCCT CCAGGGATGG ATCCTTGCTG CTGACATCAG CTACCTGGAG CCAGCCTCTG 
TCTGCACTGT GGGGAATGAA ATCAGTATTT GATATGAAGC ATTCCTTTAC AGAAGATCAT 
TATGTTGAGT TCAGTAAGCA TTCTCAGGAC CGGGTCATAG GCACGAAGGG AGACATCGCC 
CATATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG CGCCACCTTT AATCCTACAG ACGATCTTGT CTTAAATGAT 
GGCGTCCTGT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTCGACAA GTTCAACATG 
AACATCAGTG GCGTCTTCCA TCCGAACGGG CTGGAGGTCA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CCTTCCATCT TTTACACACA GTTCCTGCTC TCGATCAGTG TCGCGTGGTG 
TTCAACCACA CGGGGACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
CTATTGGAAG AGAGGATGAA AAGCCCTTTT GGATCATCCT TCCGAACATT TAATGCGACT 
GACTACAAAC CTATAGCAAC AATTGACGTG AAAAGGAACA TTTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATCGAG AACCAAGGCA GTATGGACGC GCTGAACATG 
GACACCGTGT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCGGAGGA CGAGGACGAG 
GAGGAGGACC AGGAAGAGGA AGAGCAGGAG GAAGAAGACG ACGACGAAGA CGACGACGAC 
ACTGATGATC TGGACGAGCT GGACACTGAC CAGCTGCTGG AGGCAGAGCT CGAGGAGGAC 
GACAACAACG AGAACGCGGG GGAGGATGGG GACAATGACT TCTCGCCCTC TGACGAGGAG 
CTGGCGAACC TCCTGGAGGA GGGGGAGGAC GCGGAGGACG AAGACTCAGA TGCAGACGAG 
GAAGTGGAAC TGATCCTGGG GGACACCGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTAAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DCAF1
Entrez Gene ID	785711
Full Name	DDB1 and CUL4 associated factor 1
General protein information	Preferred Names DDB1 and CUL4 associated factor 1 Names protein VPRBP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	VPRBP	XP_005632545.1
Drosophila melanogaster (fruit fly)	mahj	NP_001246451.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012134	XP_320393.4
Mus musculus (house mouse)	Vprbp	NP_001015507.1
Rattus norvegicus (Norway rat)	Vprbp	NP_001178727.1
Gallus gallus (chicken)	VPRBP	XP_004944460.1
Xenopus tropicalis (tropical clawed frog)	vprbp	XP_004914132.1
Bos taurus (cattle)	VPRBP	XP_005223061.1
Caenorhabditis elegans (roundworm)	dcaf-1	NP_501725.2
Homo sapiens (human)	VPRBP	NP_055518.1
Pan troglodytes (chimpanzee)	VPRBP	XP_001169544.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DCAF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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