Ddx25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx25
Entrez Gene ID	30959
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 25
Synonyms	AW047046,GRTH
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 Names ATP-dependent RNA helicase DDX25 DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 25 DEAD box protein 25 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25 gonadotropin-regulated testicular RNA helicase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_013932.4	NP_038960.2	ATP-dependent RNA helicase DDX25

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DDX25	NP_001033606.1
Mus musculus (house mouse)	Ddx25	NP_038960.2
Pan troglodytes (chimpanzee)	DDX25	NP_001233379.1
	DDX25	XP_001112142.1
Canis lupus familiaris (dog)	DDX25	XP_536532.3
Xenopus tropicalis (tropical clawed frog)	ddx25	NP_001016345.1
Gallus gallus (chicken)	DDX25	XP_424667.3
Homo sapiens (human)	DDX25	NP_037396.3
Rattus norvegicus (Norway rat)	Ddx25	NP_113818.2

Gene Ontology
Bibliography

Related articles in PubMed

UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.
Bao J, Vitting-Seerup K, Waage J, Tang C, Ge Y, Porse BT, Yan W
PLoS genetics12(5)e1005863(2016 May)

Elucidation of RNA binding regions of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) to transcripts of a chromatin remodeling protein essential for spermatogenesis.
Yang R, Tsai-Morris CH, Kang JH, Dufau ML
Hormone molecular biology and clinical investigation22(3)119-30(2015 Jun)

A 5'-flanking region of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) gene directs its cell-specific androgen-regulated gene expression in testicular germ cells.
Kavarthapu R, Tsai-Morris CH, Fukushima M, Pickel J, Dufau ML
Endocrinology154(6)2200-7(2013 Jun)

The mouse cytosine-5 RNA methyltransferase NSun2 is a component of the chromatoid body and required for testis differentiation.
Hussain S, Tuorto F, Menon S, Blanco S, Cox C, Flores JV, Watt S, Kudo NR, Lyko F, Frye M
Molecular and cellular biology33(8)1561-70(2013 Apr)

Role of gonadotropin regulated testicular RNA helicase (GRTH/Ddx25) on polysomal associated mRNAs in mouse testis.
Tsai-Morris CH, Sato H, Gutti R, Dufau ML
PloS one7(3)e32470(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The association of Tp2 expression via binding to the conserved RNA binding motifs of GRTH protein and the basis for understanding GRTH in the regulation of the genes essential for germ cell elongation and completion of spermatogenesis is revealed.
Title: Elucidation of RNA binding regions of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) to transcripts of a chromatin remodeling protein essential for spermatogenesis.

Data indicate that the transgenic line can be used to define requirements for cell-specific expression of the RNA helicase (GRTH/DDX25) gene.
Title: A 5'-flanking region of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) gene directs its cell-specific androgen-regulated gene expression in testicular germ cells.

GRTH regulation by androgen in Leydig cells; molecular mechanism of androgen-regulated transcription
Title: Androgen-induced activation of gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) transcription: essential role of a nonclassical androgen response element half-site.

Data show the regulatory pathways underlying GRTH action in male reproduction.
Title: Role of gonadotropin regulated testicular RNA helicase (GRTH/Ddx25) on polysomal associated mRNAs in mouse testis.

Testis-specific miRNA-469 up-regulated in gonadotropin-regulated testicular RNA helicase (GRTH/DDX25)-null mice silences transition protein 2 and protamine 2 messages at sites within coding region: implications of its role in germ cell development.
Title: Testis-specific miRNA-469 up-regulated in gonadotropin-regulated testicular RNA helicase (GRTH/DDX25)-null mice silences transition protein 2 and protamine 2 messages at sites within coding region: implications of its role in germ cell development.

The following Ddx25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10181	NM_013932.4 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx25 ( NM_013932.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038960.2 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25), mRNA.

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CloneID	OMu10181
Clone ID Related Accession (Same CDS sequence)	NM_013932.4
Accession Version	NM_013932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DDX25
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF104207.1 and AK078340.1. On Dec 22, 2007 this sequence version replaced NM_013932.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK078340.1, AK050693.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGT TACTTTGGGG AGGCGACGCC GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ATCTCGTCCA TCCCAGGAAG AACCTTCGTG GTATTAGGAG TACCACAGTC 
CCAAACATAG ACGGCTCTCT TAACACGGAA GATGATGATG ATGATGAAGA CGATGTAGTG 
GATTTGGCAG CCAATTCACT CTTAAACAAG CTAATCCGTC AGTCCTTAAT AGAATCCAGC 
CACCGAGTGG AAGTCTTACA GAAGGATCCC AGCTCTCCAC TCTACTCGGT GAAAACCTTT 
GAAGAGCTAC GGCTAAAAGA AGAATTACTA AAAGGGATTT ATGCAATGGG ATTTAACCGA 
CCATCCAAAA TCCAAGAGAT GGCCCTCCCC ATGATGCTAG CACACCCTCC CCAGAACCTC 
ATAGCACAGA GCCAGTCCGG CACAGGAAAA ACTGCTGCCT TTGTCTTAGC AATGCTAAGC 
CGAGTGAATG CCTTGGAACT GTTTCCACAG TGCCTCTGCC TGGCTCCTAC CTATGAACTG 
GCTCTGCAGA CCGGTCGTGT GGTTGAAAGG ATGGGCAAGT TCTGCGTGGA TGTTGAAGTG 
ATGTATGCCA TTCGAGGAAA CCGAATTCCA AGGGGCACCG AAGTCACCAA ACAGATTATT 
ATTGGTACTC CTGGGACTGT CCTAGATTGG TGTTTCAAGC GAAAACTGAT TGATTTGACA 
AAGATCCGTG TGTTTGTCCT GGATGAAGCA GATGTGATGA TTGACACCCA AGGATTCTCC 
GATCAGAGCA TCCGTATCCA AAGAGCTTTA CCCTCTGAAT GCCAGATGCT CCTCTTTTCG 
GCAACCTTTG AGGACTCTGT GTGGCAGTTT GCTGAACGAA TCATTCCTGA CCCCAATGTT 
ATCAAGTTGC GAAAAGAGGA ACTGACCCTG AACAACATCC GGCAGTATTA CGTGTTGTGT 
GAGAACAGGA AAGGCAAATA CCAAGCCCTC TGTAACATCT ACGGTGGCAT CACCATTGGC 
CAAGCCATTA TCTTCTGCCA GACACGTCGA AATGCCAAGT GGCTGACTGT GGAGATGATG 
CAGGATGGCC ACCAGGTGTC CCTGCTAAGT GGGGAACTCA CAGTGGAGCA GCGAGCTTCC 
ATCATTCAGA GATTCCGAGA TGGCAAAGAG AAGGTTCTCA TAACGACCAA TGTCTGTGCT 
CGAGGGATTG ATGTGAAGCA GGTCACCATC GTCGTGAACT TTGACCTCCC TGTAAACCAA 
TCAGAAGAGC CAGACTATGA GACCTACCTC CACCGTATAG GGCGGACGGG ACGCTTTGGG 
AAAAAAGGTC TCGCCTTCAA CATGATTGAA GTGGATAAGC TGCCCCTGCT TATGAAAATA 
CAAGACCACT TCAATAGCAA TATTAAGCAG CTTGACCCAG AAGACATGGA TGAGATTGAG 
AAGATCGAAT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038960.2
CDS	71..1525
Translation	MASLLWGGDAGAAESERLNSHFSNLVHPRKNLRGIRSTTVPNIDGSLNTEDDDDDEDDVVDLAANSLLNKLIRQSLIESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVERMGKFCVDVEVMYAIRGNRIPRGTEVTKQIIIGTPGTVLDWCFKRKLIDLTKIRVFVLDEADVMIDTQGFSDQSIRIQRALPSECQMLLFSATFEDSVWQFAERIIPDPNVIKLRKEELTLNNIRQYYVLCENRKGKYQALCNIYGGITIGQAIIFCQTRRNAKWLTVEMMQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVNQSEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDKLPLLMKIQDHFNSNIKQLDPEDMDEIEKIEY

Target ORF information:

RefSeq Version	NM_013932.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013932.4

ORF Insert Sequence:

ATGGCGTCGT TACTTTGGGG AGGCGACGCC GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ATCTCGTCCA TCCCAGGAAG AACCTTCGTG GTATTAGGAG TACCACAGTC 
CCAAACATAG ACGGCTCTCT TAACACGGAA GATGATGATG ATGATGAAGA CGATGTAGTG 
GATTTGGCAG CCAATTCACT CTTAAACAAG CTAATCCGTC AGTCCTTAAT AGAATCCAGC 
CACCGAGTGG AAGTCTTACA GAAGGATCCC AGCTCTCCAC TCTACTCGGT GAAAACCTTT 
GAAGAGCTAC GGCTAAAAGA AGAATTACTA AAAGGGATTT ATGCAATGGG ATTTAACCGA 
CCATCCAAAA TCCAAGAGAT GGCCCTCCCC ATGATGCTAG CACACCCTCC CCAGAACCTC 
ATAGCACAGA GCCAGTCCGG CACAGGAAAA ACTGCTGCCT TTGTCTTAGC AATGCTAAGC 
CGAGTGAATG CCTTGGAACT GTTTCCACAG TGCCTCTGCC TGGCTCCTAC CTATGAACTG 
GCTCTGCAGA CCGGTCGTGT GGTTGAAAGG ATGGGCAAGT TCTGCGTGGA TGTTGAAGTG 
ATGTATGCCA TTCGAGGAAA CCGAATTCCA AGGGGCACCG AAGTCACCAA ACAGATTATT 
ATTGGTACTC CTGGGACTGT CCTAGATTGG TGTTTCAAGC GAAAACTGAT TGATTTGACA 
AAGATCCGTG TGTTTGTCCT GGATGAAGCA GATGTGATGA TTGACACCCA AGGATTCTCC 
GATCAGAGCA TCCGTATCCA AAGAGCTTTA CCCTCTGAAT GCCAGATGCT CCTCTTTTCG 
GCAACCTTTG AGGACTCTGT GTGGCAGTTT GCTGAACGAA TCATTCCTGA CCCCAATGTT 
ATCAAGTTGC GAAAAGAGGA ACTGACCCTG AACAACATCC GGCAGTATTA CGTGTTGTGT 
GAGAACAGGA AAGGCAAATA CCAAGCCCTC TGTAACATCT ACGGTGGCAT CACCATTGGC 
CAAGCCATTA TCTTCTGCCA GACACGTCGA AATGCCAAGT GGCTGACTGT GGAGATGATG 
CAGGATGGCC ACCAGGTGTC CCTGCTAAGT GGGGAACTCA CAGTGGAGCA GCGAGCTTCC 
ATCATTCAGA GATTCCGAGA TGGCAAAGAG AAGGTTCTCA TAACGACCAA TGTCTGTGCT 
CGAGGGATTG ATGTGAAGCA GGTCACCATC GTCGTGAACT TTGACCTCCC TGTAAACCAA 
TCAGAAGAGC CAGACTATGA GACCTACCTC CACCGTATAG GGCGGACGGG ACGCTTTGGG 
AAAAAAGGTC TCGCCTTCAA CATGATTGAA GTGGATAAGC TGCCCCTGCT TATGAAAATA 
CAAGACCACT TCAATAGCAA TATTAAGCAG CTTGACCCAG AAGACATGGA TGAGATTGAG 
AAGATCGAAT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.
PLoS genetics12(5)e1005863(2016 May)
Bao J,Vitting-Seerup K,Waage J,Tang C,Ge Y,Porse BT,Yan W

Elucidation of RNA binding regions of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) to transcripts of a chromatin remodeling protein essential for spermatogenesis.
Hormone molecular biology and clinical investigation22(3)119-30(2015 Jun)
Yang R,Tsai-Morris CH,Kang JH,Dufau ML

A 5'-flanking region of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) gene directs its cell-specific androgen-regulated gene expression in testicular germ cells.
Endocrinology154(6)2200-7(2013 Jun)
Kavarthapu R,Tsai-Morris CH,Fukushima M,Pickel J,Dufau ML

The mouse cytosine-5 RNA methyltransferase NSun2 is a component of the chromatoid body and required for testis differentiation.
Molecular and cellular biology33(8)1561-70(2013 Apr)
Hussain S,Tuorto F,Menon S,Blanco S,Cox C,Flores JV,Watt S,Kudo NR,Lyko F,Frye M

Role of gonadotropin regulated testicular RNA helicase (GRTH/Ddx25) on polysomal associated mRNAs in mouse testis.
PloS one7(3)e32470(2012)
Tsai-Morris CH,Sato H,Gutti R,Dufau ML

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Ddx25 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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