DDX25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX25
Entrez Gene ID	29118
Full Name	DEAD-box helicase 25
Synonyms	GRTH
General protein information	Preferred Names DEAD-box helicase 25 Names ATP-dependent RNA helicase DDX25 DEAD (Asp-Glu-Ala-Asp) box helicase 25 DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 DEAD box protein 25 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25 gonadotropin-regulated testicular RNA helicase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q24.2
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008].
Disorder MIM:	607663

mRNA and Protein(s)

mRNA	Protein	Name
XM_011542790.2	XP_011541092.1	ATP-dependent RNA helicase DDX25 isoform X1
NM_001330438.2	NP_001317367.1	ATP-dependent RNA helicase DDX25 isoform 2
NM_013264.5	NP_037396.3	ATP-dependent RNA helicase DDX25 isoform 1
XM_011542791.2	XP_011541093.1	ATP-dependent RNA helicase DDX25 isoform X2
NM_001330438.1	NP_001317367.1	ATP-dependent RNA helicase DDX25 isoform 2
NM_013264.4	NP_037396.3	ATP-dependent RNA helicase DDX25 isoform 1
XM_011542790.3	XP_011541092.1	ATP-dependent RNA helicase DDX25 isoform X1
XM_011542791.3	XP_011541093.1	ATP-dependent RNA helicase DDX25 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DDX25	NP_001033606.1
Mus musculus (house mouse)	Ddx25	NP_038960.2
Pan troglodytes (chimpanzee)	DDX25	NP_001233379.1
	DDX25	XP_001112142.1
Canis lupus familiaris (dog)	DDX25	XP_536532.3
Xenopus tropicalis (tropical clawed frog)	ddx25	NP_001016345.1
Gallus gallus (chicken)	DDX25	XP_424667.3
Homo sapiens (human)	DDX25	NP_037396.3
Rattus norvegicus (Norway rat)	Ddx25	NP_113818.2

Gene Ontology
Bibliography

Related articles in PubMed

Association of the gonadotrophin-regulated testicular RNA helicase gene polymorphism with human male infertility.
Jaiswal D, Trivedi S, Agrawal NK, Singh K
Andrologia46(9)1063-6(2014)

Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent.
Aston KI, Krausz C, Laface I, Ruiz-Castan? E, Carrell DT
Human reproduction (Oxford, England)25(6)1383-97(2010 Jun)

Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis.
Tsai-Morris CH, Sheng Y, Gutti RK, Tang PZ, Dufau ML
Journal of andrology31(1)45-52(2010 Jan-Feb)

Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array.
Kweekel DM, Antonini NF, Nortier JW, Punt CJ, Gelderblom H, Guchelaar HJ
British journal of cancer101(2)357-62(2009 Jul)

Gonadotropin-regulated testicular helicase (GRTH/DDX25): an essential regulator of spermatogenesis.
Dufau ML, Tsai-Morris CH
Trends in endocrinology and metabolism: TEM18(8)314-20(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysed the association of GRTH gene IVS6+55G/T and c.852C/T polymorphisms with male infertility. The c.852 T allele was associated with an increased risk of male infertility (OR: 3.16, P = 0.008), whereas IVS6+55G/T allele conferred no risk.
Title: Association of the gonadotrophin-regulated testicular RNA helicase gene polymorphism with human male infertility.

Observational study of gene-disease association. (HuGE Navigator)
Title: Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent.

multifunctional RNA helicase that is an essential regulator of spermatogenesis
Title: Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array.

The association of GRTH mutations with male infertility underlines the importance of GRTH as a central, post-transcriptional regulator of spermatogenesis.
Title: Gonadotropin-regulated testicular helicase (GRTH/DDX25): an essential regulator of spermatogenesis.

The following DDX25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06441	NM_013264.5 Latest version!	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu67348	XM_011542790.3 Latest version!	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu67349	XM_011542791.3 Latest version!	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu102184	NM_001330438.2 Latest version!	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu102184	NM_001330438.1	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu06441	NM_013264.4	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu67348	XM_011542790.2	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu67349	XM_011542791.2	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

DDX25 ( NM_013264.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_037396.3 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06441
Clone ID Related Accession (Same CDS sequence)	NM_013264.5 , NM_013264.4
Accession Version	NM_013264.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC050360.1, AL563479.3, AF155140.3 and AP000842.6. On Nov 23, 2018 this sequence version replaced NM_013264.4. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.234420.1, SRR1803617.138928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263576.11/ ENSP00000263576.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCGT TACTGTGGGG AGGCGACGCA GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ACCTCAGCCA ACCCCGGAAG AACCTTTGGG GTATTAAGAG TACTGCAGTC 
CGAAACATAG ATGGTTCTAT TAATAACATC AATGAAGATG ATGAAGAAGA TGTAGTGGAT 
TTGGCAGCTA ATTCACTCTT AAACAAGTTA ATCCATCAAT CCTTAGTAGA ATCCAGTCAC 
CGTGTGGAAG TTTTACAGAA GGATCCCAGC TCTCCACTTT ACTCAGTAAA GACATTTGAA 
GAGCTGCGGC TAAAGGAAGA GTTACTAAAA GGAATCTATG CAATGGGATT TAATAGGCCA 
TCTAAAATCC AAGAGATGGC TCTCCCTATG ATGCTGGCAC ATCCACCCCA GAACCTCATA 
GCACAGAGCC AGTCTGGAAC AGGAAAGACA GCGGCATTTG TGTTGGCAAT GTTAAGCAGA 
GTTAATGCCT TGGAATTGTT CCCACAGTGC CTCTGCCTAG CTCCTACTTA TGAATTGGCT 
CTGCAAACTG GCCGTGTGGT TGAGCAGATG GGAAAATTCT GTGTGGATGT TCAAGTGATG 
TATGCCATTC GAGGGAATCG AATTCCCAGA GGCACCGACA TCACTAAACA GATTATAATT 
GGCACTCCTG GGACTGTCCT AGATTGGTGT TTTAAACTAA AATTGATTGA TTTGACTAAG 
ATTCGTGTGT TTGTCCTGGA TGAAGCAGAT GTGATGATTG ACACTCAAGG ATTCTCAGAT 
CATAGTATTC GTATTCAAAG AGCTCTACCC TCCGAATGCC AAATGCTCCT CTTTTCAGCA 
ACCTTTGAGG ACTCTGTGTG GCACTTTGCT GAGCGAATCA TCCCTGACCC TAATGTTATC 
AAGTTACGCA AAGAGGAGCT CACACTGAAC AACATCCGGC AATATTACGT GCTGTGTGAG 
CACAGGAAAG ACAAATACCA AGCTCTGTGC AACATTTATG GCAGCATCAC CATTGGTCAG 
GCCATCATCT TCTGCCAGAC TCGTCGAAAC GCTAAGTGGT TGACCGTGGA GATGATACAG 
GATGGCCACC AGGTGTCTTT GTTAAGCGGG GAGCTGACCG TGGAGCAGCG AGCTTCCATC 
ATTCAGAGGT TTCGGGATGG GAAAGAGAAG GTTCTCATAA CAACTAATGT TTGTGCCCGA 
GGGATTGATG TGAAGCAGGT CACAATTGTT GTGAACTTTG ATCTCCCTGT AAAACAAGGA 
GAGGAGCCGG ACTATGAGAC CTACCTCCAC CGCATAGGGC GGACGGGGCG CTTTGGGAAA 
AAAGGCCTTG CCTTCAACAT GATTGAAGTA GATGAGCTGC CCTCGCTCAT GAAAATCCAG 
GACCACTTTA ACAGCAGTAT TAAGCAACTC AACGCTGAAG ACATGGATGA AATTGAAAAG 
ATTGACTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037396.3
CDS	11..1462
Translation	MASLLWGGDAGAAESERLNSHFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	NM_013264.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013264.5

ORF Insert Sequence:

ATGGCGTCGT TACTGTGGGG AGGCGACGCA GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ACCTCAGCCA ACCCCGGAAG AACCTTTGGG GTATTAAGAG TACTGCAGTC 
CGAAACATAG ATGGTTCTAT TAATAACATC AATGAAGATG ATGAAGAAGA TGTAGTGGAT 
TTGGCAGCTA ATTCACTCTT AAACAAGTTA ATCCATCAAT CCTTAGTAGA ATCCAGTCAC 
CGTGTGGAAG TTTTACAGAA GGATCCCAGC TCTCCACTTT ACTCAGTAAA GACATTTGAA 
GAGCTGCGGC TAAAGGAAGA GTTACTAAAA GGAATCTATG CAATGGGATT TAATAGGCCA 
TCTAAAATCC AAGAGATGGC TCTCCCTATG ATGCTGGCAC ATCCACCCCA GAACCTCATA 
GCACAGAGCC AGTCTGGAAC AGGAAAGACA GCGGCATTTG TGTTGGCAAT GTTAAGCAGA 
GTTAATGCCT TGGAATTGTT CCCACAGTGC CTCTGCCTAG CTCCTACTTA TGAATTGGCT 
CTGCAAACTG GCCGTGTGGT TGAGCAGATG GGAAAATTCT GTGTGGATGT TCAAGTGATG 
TATGCCATTC GAGGGAATCG AATTCCCAGA GGCACCGACA TCACTAAACA GATTATAATT 
GGCACTCCTG GGACTGTCCT AGATTGGTGT TTTAAACTAA AATTGATTGA TTTGACTAAG 
ATTCGTGTGT TTGTCCTGGA TGAAGCAGAT GTGATGATTG ACACTCAAGG ATTCTCAGAT 
CATAGTATTC GTATTCAAAG AGCTCTACCC TCCGAATGCC AAATGCTCCT CTTTTCAGCA 
ACCTTTGAGG ACTCTGTGTG GCACTTTGCT GAGCGAATCA TCCCTGACCC TAATGTTATC 
AAGTTACGCA AAGAGGAGCT CACACTGAAC AACATCCGGC AATATTACGT GCTGTGTGAG 
CACAGGAAAG ACAAATACCA AGCTCTGTGC AACATTTATG GCAGCATCAC CATTGGTCAG 
GCCATCATCT TCTGCCAGAC TCGTCGAAAC GCTAAGTGGT TGACCGTGGA GATGATACAG 
GATGGCCACC AGGTGTCTTT GTTAAGCGGG GAGCTGACCG TGGAGCAGCG AGCTTCCATC 
ATTCAGAGGT TTCGGGATGG GAAAGAGAAG GTTCTCATAA CAACTAATGT TTGTGCCCGA 
GGGATTGATG TGAAGCAGGT CACAATTGTT GTGAACTTTG ATCTCCCTGT AAAACAAGGA 
GAGGAGCCGG ACTATGAGAC CTACCTCCAC CGCATAGGGC GGACGGGGCG CTTTGGGAAA 
AAAGGCCTTG CCTTCAACAT GATTGAAGTA GATGAGCTGC CCTCGCTCAT GAAAATCCAG 
GACCACTTTA ACAGCAGTAT TAAGCAACTC AACGCTGAAG ACATGGATGA AATTGAAAAG 
ATTGACTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( NM_013264.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037396.3 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06441
Clone ID Related Accession (Same CDS sequence)	NM_013264.5 , NM_013264.4
Accession Version	NM_013264.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395868.1, CD359894.1, BC050360.1, CD657404.1 and AF155140.2. On Mar 16, 2012 this sequence version replaced NM_013264.3. ##Evidence-Data-START## Transcript exon combination :: BC031098.1, SRR1803613.279774.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGT TACTGTGGGG AGGCGACGCA GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ACCTCAGCCA ACCCCGGAAG AACCTTTGGG GTATTAAGAG TACTGCAGTC 
CGAAACATAG ATGGTTCTAT TAATAACATC AATGAAGATG ATGAAGAAGA TGTAGTGGAT 
TTGGCAGCTA ATTCACTCTT AAACAAGTTA ATCCATCAAT CCTTAGTAGA ATCCAGTCAC 
CGTGTGGAAG TTTTACAGAA GGATCCCAGC TCTCCACTTT ACTCAGTAAA GACATTTGAA 
GAGCTGCGGC TAAAGGAAGA GTTACTAAAA GGAATCTATG CAATGGGATT TAATAGGCCA 
TCTAAAATCC AAGAGATGGC TCTCCCTATG ATGCTGGCAC ATCCACCCCA GAACCTCATA 
GCACAGAGCC AGTCTGGAAC AGGAAAGACA GCGGCATTTG TGTTGGCAAT GTTAAGCAGA 
GTTAATGCCT TGGAATTGTT CCCACAGTGC CTCTGCCTAG CTCCTACTTA TGAATTGGCT 
CTGCAAACTG GCCGTGTGGT TGAGCAGATG GGAAAATTCT GTGTGGATGT TCAAGTGATG 
TATGCCATTC GAGGGAATCG AATTCCCAGA GGCACCGACA TCACTAAACA GATTATAATT 
GGCACTCCTG GGACTGTCCT AGATTGGTGT TTTAAACTAA AATTGATTGA TTTGACTAAG 
ATTCGTGTGT TTGTCCTGGA TGAAGCAGAT GTGATGATTG ACACTCAAGG ATTCTCAGAT 
CATAGTATTC GTATTCAAAG AGCTCTACCC TCCGAATGCC AAATGCTCCT CTTTTCAGCA 
ACCTTTGAGG ACTCTGTGTG GCACTTTGCT GAGCGAATCA TCCCTGACCC TAATGTTATC 
AAGTTACGCA AAGAGGAGCT CACACTGAAC AACATCCGGC AATATTACGT GCTGTGTGAG 
CACAGGAAAG ACAAATACCA AGCTCTGTGC AACATTTATG GCAGCATCAC CATTGGTCAG 
GCCATCATCT TCTGCCAGAC TCGTCGAAAC GCTAAGTGGT TGACCGTGGA GATGATACAG 
GATGGCCACC AGGTGTCTTT GTTAAGCGGG GAGCTGACCG TGGAGCAGCG AGCTTCCATC 
ATTCAGAGGT TTCGGGATGG GAAAGAGAAG GTTCTCATAA CAACTAATGT TTGTGCCCGA 
GGGATTGATG TGAAGCAGGT CACAATTGTT GTGAACTTTG ATCTCCCTGT AAAACAAGGA 
GAGGAGCCGG ACTATGAGAC CTACCTCCAC CGCATAGGGC GGACGGGGCG CTTTGGGAAA 
AAAGGCCTTG CCTTCAACAT GATTGAAGTA GATGAGCTGC CCTCGCTCAT GAAAATCCAG 
GACCACTTTA ACAGCAGTAT TAAGCAACTC AACGCTGAAG ACATGGATGA AATTGAAAAG 
ATTGACTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037396.3
CDS	153..1604
Translation	MASLLWGGDAGAAESERLNSHFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	NM_013264.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013264.4

ORF Insert Sequence:

ATGGCGTCGT TACTGTGGGG AGGCGACGCA GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ACCTCAGCCA ACCCCGGAAG AACCTTTGGG GTATTAAGAG TACTGCAGTC 
CGAAACATAG ATGGTTCTAT TAATAACATC AATGAAGATG ATGAAGAAGA TGTAGTGGAT 
TTGGCAGCTA ATTCACTCTT AAACAAGTTA ATCCATCAAT CCTTAGTAGA ATCCAGTCAC 
CGTGTGGAAG TTTTACAGAA GGATCCCAGC TCTCCACTTT ACTCAGTAAA GACATTTGAA 
GAGCTGCGGC TAAAGGAAGA GTTACTAAAA GGAATCTATG CAATGGGATT TAATAGGCCA 
TCTAAAATCC AAGAGATGGC TCTCCCTATG ATGCTGGCAC ATCCACCCCA GAACCTCATA 
GCACAGAGCC AGTCTGGAAC AGGAAAGACA GCGGCATTTG TGTTGGCAAT GTTAAGCAGA 
GTTAATGCCT TGGAATTGTT CCCACAGTGC CTCTGCCTAG CTCCTACTTA TGAATTGGCT 
CTGCAAACTG GCCGTGTGGT TGAGCAGATG GGAAAATTCT GTGTGGATGT TCAAGTGATG 
TATGCCATTC GAGGGAATCG AATTCCCAGA GGCACCGACA TCACTAAACA GATTATAATT 
GGCACTCCTG GGACTGTCCT AGATTGGTGT TTTAAACTAA AATTGATTGA TTTGACTAAG 
ATTCGTGTGT TTGTCCTGGA TGAAGCAGAT GTGATGATTG ACACTCAAGG ATTCTCAGAT 
CATAGTATTC GTATTCAAAG AGCTCTACCC TCCGAATGCC AAATGCTCCT CTTTTCAGCA 
ACCTTTGAGG ACTCTGTGTG GCACTTTGCT GAGCGAATCA TCCCTGACCC TAATGTTATC 
AAGTTACGCA AAGAGGAGCT CACACTGAAC AACATCCGGC AATATTACGT GCTGTGTGAG 
CACAGGAAAG ACAAATACCA AGCTCTGTGC AACATTTATG GCAGCATCAC CATTGGTCAG 
GCCATCATCT TCTGCCAGAC TCGTCGAAAC GCTAAGTGGT TGACCGTGGA GATGATACAG 
GATGGCCACC AGGTGTCTTT GTTAAGCGGG GAGCTGACCG TGGAGCAGCG AGCTTCCATC 
ATTCAGAGGT TTCGGGATGG GAAAGAGAAG GTTCTCATAA CAACTAATGT TTGTGCCCGA 
GGGATTGATG TGAAGCAGGT CACAATTGTT GTGAACTTTG ATCTCCCTGT AAAACAAGGA 
GAGGAGCCGG ACTATGAGAC CTACCTCCAC CGCATAGGGC GGACGGGGCG CTTTGGGAAA 
AAAGGCCTTG CCTTCAACAT GATTGAAGTA GATGAGCTGC CCTCGCTCAT GAAAATCCAG 
GACCACTTTA ACAGCAGTAT TAAGCAACTC AACGCTGAAG ACATGGATGA AATTGAAAAG 
ATTGACTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( XM_011542790.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541092.1 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67348
Clone ID Related Accession (Same CDS sequence)	XM_011542790.2 , XM_011542790.3
Accession Version	XM_011542790.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
GCAAGACCTT TGTTTTCAAA CCTCAGCCAA CCCCGGAAGA ACCTTTGGGG TATTAAGAGT 
ACTGCAGTCC GAAACATAGA TGGTTCTATT AATAACATCA ATGAAGATGA TGAAGAAGAT 
GTAGTGGATT TGGCAGCTAA TTCACTCTTA AACAAGTTAA TCCATCAATC CTTAGTAGAA 
TCCAGTCACC GTGTGGAAGT TTTACAGAAG GATCCCAGCT CTCCACTTTA CTCAGTAAAG 
ACATTTGAAG AGCTGCGGCT AAAGGAAGAG TTACTAAAAG GAATCTATGC AATGGGATTT 
AATAGGCCAT CTAAAATCCA AGAGATGGCT CTCCCTATGA TGCTGGCACA TCCACCCCAG 
AACCTCATAG CACAGAGCCA GTCTGGAACA GGAAAGACAG CGGCATTTGT GTTGGCAATG 
TTAAGCAGAG TTAATGCCTT GGAATTGTTC CCACAGTGCC TCTGCCTAGC TCCTACTTAT 
GAATTGGCTC TGCAAACTGG CCGTGTGGTT GAGCAGATGG GAAAATTCTG TGTGGATGTT 
CAAGTGATGT ATGCCATTCG AGGGAATCGA ATTCCCAGAG GCACCGACAT CACTAAACAG 
ATTATAATTG GCACTCCTGG GACTGTCCTA GATTGGTGTT TTAAACTAAA ATTGATTGAT 
TTGACTAAGA TTCGTGTGTT TGTCCTGGAT GAAGCAGATG TGATGATTGA CACTCAAGGA 
TTCTCAGATC ATAGTATTCG TATTCAAAGA GCTCTACCCT CCGAATGCCA AATGCTCCTC 
TTTTCAGCAA CCTTTGAGGA CTCTGTGTGG CACTTTGCTG AGCGAATCAT CCCTGACCCT 
AATGTTATCA AGTTACGCAA AGAGGAGCTC ACACTGAACA ACATCCGGCA ATATTACGTG 
CTGTGTGAGC ACAGGAAAGA CAAATACCAA GCTCTGTGCA ACATTTATGG CAGCATCACC 
ATTGGTCAGG CCATCATCTT CTGCCAGACT CGTCGAAACG CTAAGTGGTT GACCGTGGAG 
ATGATACAGG ATGGCCACCA GGTGTCTTTG TTAAGCGGGG AGCTGACCGT GGAGCAGCGA 
GCTTCCATCA TTCAGAGGTT TCGGGATGGG AAAGAGAAGG TTCTCATAAC AACTAATGTT 
TGTGCCCGAG GGATTGATGT GAAGCAGGTC ACAATTGTTG TGAACTTTGA TCTCCCTGTA 
AAACAAGGAG AGGAGCCGGA CTATGAGACC TACCTCCACC GCATAGGGCG GACGGGGCGC 
TTTGGGAAAA AAGGCCTTGC CTTCAACATG ATTGAAGTAG ATGAGCTGCC CTCGCTCATG 
AAAATCCAGG ACCACTTTAA CAGCAGTATT AAGCAACTCA ACGCTGAAGA CATGGATGAA 
ATTGAAAAGA TTGACTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541092.1
CDS	48..1508
Translation	MREGFGRGPQRETPSPPPREARPLFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	XM_011542790.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542790.2

ORF Insert Sequence:

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
GCAAGACCTT TGTTTTCAAA CCTCAGCCAA CCCCGGAAGA ACCTTTGGGG TATTAAGAGT 
ACTGCAGTCC GAAACATAGA TGGTTCTATT AATAACATCA ATGAAGATGA TGAAGAAGAT 
GTAGTGGATT TGGCAGCTAA TTCACTCTTA AACAAGTTAA TCCATCAATC CTTAGTAGAA 
TCCAGTCACC GTGTGGAAGT TTTACAGAAG GATCCCAGCT CTCCACTTTA CTCAGTAAAG 
ACATTTGAAG AGCTGCGGCT AAAGGAAGAG TTACTAAAAG GAATCTATGC AATGGGATTT 
AATAGGCCAT CTAAAATCCA AGAGATGGCT CTCCCTATGA TGCTGGCACA TCCACCCCAG 
AACCTCATAG CACAGAGCCA GTCTGGAACA GGAAAGACAG CGGCATTTGT GTTGGCAATG 
TTAAGCAGAG TTAATGCCTT GGAATTGTTC CCACAGTGCC TCTGCCTAGC TCCTACTTAT 
GAATTGGCTC TGCAAACTGG CCGTGTGGTT GAGCAGATGG GAAAATTCTG TGTGGATGTT 
CAAGTGATGT ATGCCATTCG AGGGAATCGA ATTCCCAGAG GCACCGACAT CACTAAACAG 
ATTATAATTG GCACTCCTGG GACTGTCCTA GATTGGTGTT TTAAACTAAA ATTGATTGAT 
TTGACTAAGA TTCGTGTGTT TGTCCTGGAT GAAGCAGATG TGATGATTGA CACTCAAGGA 
TTCTCAGATC ATAGTATTCG TATTCAAAGA GCTCTACCCT CCGAATGCCA AATGCTCCTC 
TTTTCAGCAA CCTTTGAGGA CTCTGTGTGG CACTTTGCTG AGCGAATCAT CCCTGACCCT 
AATGTTATCA AGTTACGCAA AGAGGAGCTC ACACTGAACA ACATCCGGCA ATATTACGTG 
CTGTGTGAGC ACAGGAAAGA CAAATACCAA GCTCTGTGCA ACATTTATGG CAGCATCACC 
ATTGGTCAGG CCATCATCTT CTGCCAGACT CGTCGAAACG CTAAGTGGTT GACCGTGGAG 
ATGATACAGG ATGGCCACCA GGTGTCTTTG TTAAGCGGGG AGCTGACCGT GGAGCAGCGA 
GCTTCCATCA TTCAGAGGTT TCGGGATGGG AAAGAGAAGG TTCTCATAAC AACTAATGTT 
TGTGCCCGAG GGATTGATGT GAAGCAGGTC ACAATTGTTG TGAACTTTGA TCTCCCTGTA 
AAACAAGGAG AGGAGCCGGA CTATGAGACC TACCTCCACC GCATAGGGCG GACGGGGCGC 
TTTGGGAAAA AAGGCCTTGC CTTCAACATG ATTGAAGTAG ATGAGCTGCC CTCGCTCATG 
AAAATCCAGG ACCACTTTAA CAGCAGTATT AAGCAACTCA ACGCTGAAGA CATGGATGAA 
ATTGAAAAGA TTGACTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( XM_011542790.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541092.1 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67348
Clone ID Related Accession (Same CDS sequence)	XM_011542790.2 , XM_011542790.3
Accession Version	XM_011542790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011542790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
GCAAGACCTT TGTTTTCAAA CCTCAGCCAA CCCCGGAAGA ACCTTTGGGG TATTAAGAGT 
ACTGCAGTCC GAAACATAGA TGGTTCTATT AATAACATCA ATGAAGATGA TGAAGAAGAT 
GTAGTGGATT TGGCAGCTAA TTCACTCTTA AACAAGTTAA TCCATCAATC CTTAGTAGAA 
TCCAGTCACC GTGTGGAAGT TTTACAGAAG GATCCCAGCT CTCCACTTTA CTCAGTAAAG 
ACATTTGAAG AGCTGCGGCT AAAGGAAGAG TTACTAAAAG GAATCTATGC AATGGGATTT 
AATAGGCCAT CTAAAATCCA AGAGATGGCT CTCCCTATGA TGCTGGCACA TCCACCCCAG 
AACCTCATAG CACAGAGCCA GTCTGGAACA GGAAAGACAG CGGCATTTGT GTTGGCAATG 
TTAAGCAGAG TTAATGCCTT GGAATTGTTC CCACAGTGCC TCTGCCTAGC TCCTACTTAT 
GAATTGGCTC TGCAAACTGG CCGTGTGGTT GAGCAGATGG GAAAATTCTG TGTGGATGTT 
CAAGTGATGT ATGCCATTCG AGGGAATCGA ATTCCCAGAG GCACCGACAT CACTAAACAG 
ATTATAATTG GCACTCCTGG GACTGTCCTA GATTGGTGTT TTAAACTAAA ATTGATTGAT 
TTGACTAAGA TTCGTGTGTT TGTCCTGGAT GAAGCAGATG TGATGATTGA CACTCAAGGA 
TTCTCAGATC ATAGTATTCG TATTCAAAGA GCTCTACCCT CCGAATGCCA AATGCTCCTC 
TTTTCAGCAA CCTTTGAGGA CTCTGTGTGG CACTTTGCTG AGCGAATCAT CCCTGACCCT 
AATGTTATCA AGTTACGCAA AGAGGAGCTC ACACTGAACA ACATCCGGCA ATATTACGTG 
CTGTGTGAGC ACAGGAAAGA CAAATACCAA GCTCTGTGCA ACATTTATGG CAGCATCACC 
ATTGGTCAGG CCATCATCTT CTGCCAGACT CGTCGAAACG CTAAGTGGTT GACCGTGGAG 
ATGATACAGG ATGGCCACCA GGTGTCTTTG TTAAGCGGGG AGCTGACCGT GGAGCAGCGA 
GCTTCCATCA TTCAGAGGTT TCGGGATGGG AAAGAGAAGG TTCTCATAAC AACTAATGTT 
TGTGCCCGAG GGATTGATGT GAAGCAGGTC ACAATTGTTG TGAACTTTGA TCTCCCTGTA 
AAACAAGGAG AGGAGCCGGA CTATGAGACC TACCTCCACC GCATAGGGCG GACGGGGCGC 
TTTGGGAAAA AAGGCCTTGC CTTCAACATG ATTGAAGTAG ATGAGCTGCC CTCGCTCATG 
AAAATCCAGG ACCACTTTAA CAGCAGTATT AAGCAACTCA ACGCTGAAGA CATGGATGAA 
ATTGAAAAGA TTGACTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541092.1
CDS	41..1501
Translation	MREGFGRGPQRETPSPPPREARPLFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	XM_011542790.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542790.3

ORF Insert Sequence:

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
GCAAGACCTT TGTTTTCAAA CCTCAGCCAA CCCCGGAAGA ACCTTTGGGG TATTAAGAGT 
ACTGCAGTCC GAAACATAGA TGGTTCTATT AATAACATCA ATGAAGATGA TGAAGAAGAT 
GTAGTGGATT TGGCAGCTAA TTCACTCTTA AACAAGTTAA TCCATCAATC CTTAGTAGAA 
TCCAGTCACC GTGTGGAAGT TTTACAGAAG GATCCCAGCT CTCCACTTTA CTCAGTAAAG 
ACATTTGAAG AGCTGCGGCT AAAGGAAGAG TTACTAAAAG GAATCTATGC AATGGGATTT 
AATAGGCCAT CTAAAATCCA AGAGATGGCT CTCCCTATGA TGCTGGCACA TCCACCCCAG 
AACCTCATAG CACAGAGCCA GTCTGGAACA GGAAAGACAG CGGCATTTGT GTTGGCAATG 
TTAAGCAGAG TTAATGCCTT GGAATTGTTC CCACAGTGCC TCTGCCTAGC TCCTACTTAT 
GAATTGGCTC TGCAAACTGG CCGTGTGGTT GAGCAGATGG GAAAATTCTG TGTGGATGTT 
CAAGTGATGT ATGCCATTCG AGGGAATCGA ATTCCCAGAG GCACCGACAT CACTAAACAG 
ATTATAATTG GCACTCCTGG GACTGTCCTA GATTGGTGTT TTAAACTAAA ATTGATTGAT 
TTGACTAAGA TTCGTGTGTT TGTCCTGGAT GAAGCAGATG TGATGATTGA CACTCAAGGA 
TTCTCAGATC ATAGTATTCG TATTCAAAGA GCTCTACCCT CCGAATGCCA AATGCTCCTC 
TTTTCAGCAA CCTTTGAGGA CTCTGTGTGG CACTTTGCTG AGCGAATCAT CCCTGACCCT 
AATGTTATCA AGTTACGCAA AGAGGAGCTC ACACTGAACA ACATCCGGCA ATATTACGTG 
CTGTGTGAGC ACAGGAAAGA CAAATACCAA GCTCTGTGCA ACATTTATGG CAGCATCACC 
ATTGGTCAGG CCATCATCTT CTGCCAGACT CGTCGAAACG CTAAGTGGTT GACCGTGGAG 
ATGATACAGG ATGGCCACCA GGTGTCTTTG TTAAGCGGGG AGCTGACCGT GGAGCAGCGA 
GCTTCCATCA TTCAGAGGTT TCGGGATGGG AAAGAGAAGG TTCTCATAAC AACTAATGTT 
TGTGCCCGAG GGATTGATGT GAAGCAGGTC ACAATTGTTG TGAACTTTGA TCTCCCTGTA 
AAACAAGGAG AGGAGCCGGA CTATGAGACC TACCTCCACC GCATAGGGCG GACGGGGCGC 
TTTGGGAAAA AAGGCCTTGC CTTCAACATG ATTGAAGTAG ATGAGCTGCC CTCGCTCATG 
AAAATCCAGG ACCACTTTAA CAGCAGTATT AAGCAACTCA ACGCTGAAGA CATGGATGAA 
ATTGAAAAGA TTGACTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( XM_011542791.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541093.1 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67349
Clone ID Related Accession (Same CDS sequence)	XM_011542791.2 , XM_011542791.3
Accession Version	XM_011542791.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
TTTTCAAACC TCAGCCAACC CCGGAAGAAC CTTTGGGGTA TTAAGAGTAC TGCAGTCCGA 
AACATAGATG GTTCTATTAA TAACATCAAT GAAGATGATG AAGAAGATGT AGTGGATTTG 
GCAGCTAATT CACTCTTAAA CAAGTTAATC CATCAATCCT TAGTAGAATC CAGTCACCGT 
GTGGAAGTTT TACAGAAGGA TCCCAGCTCT CCACTTTACT CAGTAAAGAC ATTTGAAGAG 
CTGCGGCTAA AGGAAGAGTT ACTAAAAGGA ATCTATGCAA TGGGATTTAA TAGGCCATCT 
AAAATCCAAG AGATGGCTCT CCCTATGATG CTGGCACATC CACCCCAGAA CCTCATAGCA 
CAGAGCCAGT CTGGAACAGG AAAGACAGCG GCATTTGTGT TGGCAATGTT AAGCAGAGTT 
AATGCCTTGG AATTGTTCCC ACAGTGCCTC TGCCTAGCTC CTACTTATGA ATTGGCTCTG 
CAAACTGGCC GTGTGGTTGA GCAGATGGGA AAATTCTGTG TGGATGTTCA AGTGATGTAT 
GCCATTCGAG GGAATCGAAT TCCCAGAGGC ACCGACATCA CTAAACAGAT TATAATTGGC 
ACTCCTGGGA CTGTCCTAGA TTGGTGTTTT AAACTAAAAT TGATTGATTT GACTAAGATT 
CGTGTGTTTG TCCTGGATGA AGCAGATGTG ATGATTGACA CTCAAGGATT CTCAGATCAT 
AGTATTCGTA TTCAAAGAGC TCTACCCTCC GAATGCCAAA TGCTCCTCTT TTCAGCAACC 
TTTGAGGACT CTGTGTGGCA CTTTGCTGAG CGAATCATCC CTGACCCTAA TGTTATCAAG 
TTACGCAAAG AGGAGCTCAC ACTGAACAAC ATCCGGCAAT ATTACGTGCT GTGTGAGCAC 
AGGAAAGACA AATACCAAGC TCTGTGCAAC ATTTATGGCA GCATCACCAT TGGTCAGGCC 
ATCATCTTCT GCCAGACTCG TCGAAACGCT AAGTGGTTGA CCGTGGAGAT GATACAGGAT 
GGCCACCAGG TGTCTTTGTT AAGCGGGGAG CTGACCGTGG AGCAGCGAGC TTCCATCATT 
CAGAGGTTTC GGGATGGGAA AGAGAAGGTT CTCATAACAA CTAATGTTTG TGCCCGAGGG 
ATTGATGTGA AGCAGGTCAC AATTGTTGTG AACTTTGATC TCCCTGTAAA ACAAGGAGAG 
GAGCCGGACT ATGAGACCTA CCTCCACCGC ATAGGGCGGA CGGGGCGCTT TGGGAAAAAA 
GGCCTTGCCT TCAACATGAT TGAAGTAGAT GAGCTGCCCT CGCTCATGAA AATCCAGGAC 
CACTTTAACA GCAGTATTAA GCAACTCAAC GCTGAAGACA TGGATGAAAT TGAAAAGATT 
GACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541093.1
CDS	108..1556
Translation	MREGFGRGPQRETPSPPPREFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	XM_011542791.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542791.2

ORF Insert Sequence:

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
TTTTCAAACC TCAGCCAACC CCGGAAGAAC CTTTGGGGTA TTAAGAGTAC TGCAGTCCGA 
AACATAGATG GTTCTATTAA TAACATCAAT GAAGATGATG AAGAAGATGT AGTGGATTTG 
GCAGCTAATT CACTCTTAAA CAAGTTAATC CATCAATCCT TAGTAGAATC CAGTCACCGT 
GTGGAAGTTT TACAGAAGGA TCCCAGCTCT CCACTTTACT CAGTAAAGAC ATTTGAAGAG 
CTGCGGCTAA AGGAAGAGTT ACTAAAAGGA ATCTATGCAA TGGGATTTAA TAGGCCATCT 
AAAATCCAAG AGATGGCTCT CCCTATGATG CTGGCACATC CACCCCAGAA CCTCATAGCA 
CAGAGCCAGT CTGGAACAGG AAAGACAGCG GCATTTGTGT TGGCAATGTT AAGCAGAGTT 
AATGCCTTGG AATTGTTCCC ACAGTGCCTC TGCCTAGCTC CTACTTATGA ATTGGCTCTG 
CAAACTGGCC GTGTGGTTGA GCAGATGGGA AAATTCTGTG TGGATGTTCA AGTGATGTAT 
GCCATTCGAG GGAATCGAAT TCCCAGAGGC ACCGACATCA CTAAACAGAT TATAATTGGC 
ACTCCTGGGA CTGTCCTAGA TTGGTGTTTT AAACTAAAAT TGATTGATTT GACTAAGATT 
CGTGTGTTTG TCCTGGATGA AGCAGATGTG ATGATTGACA CTCAAGGATT CTCAGATCAT 
AGTATTCGTA TTCAAAGAGC TCTACCCTCC GAATGCCAAA TGCTCCTCTT TTCAGCAACC 
TTTGAGGACT CTGTGTGGCA CTTTGCTGAG CGAATCATCC CTGACCCTAA TGTTATCAAG 
TTACGCAAAG AGGAGCTCAC ACTGAACAAC ATCCGGCAAT ATTACGTGCT GTGTGAGCAC 
AGGAAAGACA AATACCAAGC TCTGTGCAAC ATTTATGGCA GCATCACCAT TGGTCAGGCC 
ATCATCTTCT GCCAGACTCG TCGAAACGCT AAGTGGTTGA CCGTGGAGAT GATACAGGAT 
GGCCACCAGG TGTCTTTGTT AAGCGGGGAG CTGACCGTGG AGCAGCGAGC TTCCATCATT 
CAGAGGTTTC GGGATGGGAA AGAGAAGGTT CTCATAACAA CTAATGTTTG TGCCCGAGGG 
ATTGATGTGA AGCAGGTCAC AATTGTTGTG AACTTTGATC TCCCTGTAAA ACAAGGAGAG 
GAGCCGGACT ATGAGACCTA CCTCCACCGC ATAGGGCGGA CGGGGCGCTT TGGGAAAAAA 
GGCCTTGCCT TCAACATGAT TGAAGTAGAT GAGCTGCCCT CGCTCATGAA AATCCAGGAC 
CACTTTAACA GCAGTATTAA GCAACTCAAC GCTGAAGACA TGGATGAAAT TGAAAAGATT 
GACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( XM_011542791.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541093.1 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67349
Clone ID Related Accession (Same CDS sequence)	XM_011542791.2 , XM_011542791.3
Accession Version	XM_011542791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011542791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
TTTTCAAACC TCAGCCAACC CCGGAAGAAC CTTTGGGGTA TTAAGAGTAC TGCAGTCCGA 
AACATAGATG GTTCTATTAA TAACATCAAT GAAGATGATG AAGAAGATGT AGTGGATTTG 
GCAGCTAATT CACTCTTAAA CAAGTTAATC CATCAATCCT TAGTAGAATC CAGTCACCGT 
GTGGAAGTTT TACAGAAGGA TCCCAGCTCT CCACTTTACT CAGTAAAGAC ATTTGAAGAG 
CTGCGGCTAA AGGAAGAGTT ACTAAAAGGA ATCTATGCAA TGGGATTTAA TAGGCCATCT 
AAAATCCAAG AGATGGCTCT CCCTATGATG CTGGCACATC CACCCCAGAA CCTCATAGCA 
CAGAGCCAGT CTGGAACAGG AAAGACAGCG GCATTTGTGT TGGCAATGTT AAGCAGAGTT 
AATGCCTTGG AATTGTTCCC ACAGTGCCTC TGCCTAGCTC CTACTTATGA ATTGGCTCTG 
CAAACTGGCC GTGTGGTTGA GCAGATGGGA AAATTCTGTG TGGATGTTCA AGTGATGTAT 
GCCATTCGAG GGAATCGAAT TCCCAGAGGC ACCGACATCA CTAAACAGAT TATAATTGGC 
ACTCCTGGGA CTGTCCTAGA TTGGTGTTTT AAACTAAAAT TGATTGATTT GACTAAGATT 
CGTGTGTTTG TCCTGGATGA AGCAGATGTG ATGATTGACA CTCAAGGATT CTCAGATCAT 
AGTATTCGTA TTCAAAGAGC TCTACCCTCC GAATGCCAAA TGCTCCTCTT TTCAGCAACC 
TTTGAGGACT CTGTGTGGCA CTTTGCTGAG CGAATCATCC CTGACCCTAA TGTTATCAAG 
TTACGCAAAG AGGAGCTCAC ACTGAACAAC ATCCGGCAAT ATTACGTGCT GTGTGAGCAC 
AGGAAAGACA AATACCAAGC TCTGTGCAAC ATTTATGGCA GCATCACCAT TGGTCAGGCC 
ATCATCTTCT GCCAGACTCG TCGAAACGCT AAGTGGTTGA CCGTGGAGAT GATACAGGAT 
GGCCACCAGG TGTCTTTGTT AAGCGGGGAG CTGACCGTGG AGCAGCGAGC TTCCATCATT 
CAGAGGTTTC GGGATGGGAA AGAGAAGGTT CTCATAACAA CTAATGTTTG TGCCCGAGGG 
ATTGATGTGA AGCAGGTCAC AATTGTTGTG AACTTTGATC TCCCTGTAAA ACAAGGAGAG 
GAGCCGGACT ATGAGACCTA CCTCCACCGC ATAGGGCGGA CGGGGCGCTT TGGGAAAAAA 
GGCCTTGCCT TCAACATGAT TGAAGTAGAT GAGCTGCCCT CGCTCATGAA AATCCAGGAC 
CACTTTAACA GCAGTATTAA GCAACTCAAC GCTGAAGACA TGGATGAAAT TGAAAAGATT 
GACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541093.1
CDS	164..1612
Translation	MREGFGRGPQRETPSPPPREFSNLSQPRKNLWGIKSTAVRNIDGSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	XM_011542791.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542791.3

ORF Insert Sequence:

ATGCGGGAAG GGTTTGGAAG AGGGCCACAG AGGGAGACCC CGTCCCCACC CCCACGAGAG 
TTTTCAAACC TCAGCCAACC CCGGAAGAAC CTTTGGGGTA TTAAGAGTAC TGCAGTCCGA 
AACATAGATG GTTCTATTAA TAACATCAAT GAAGATGATG AAGAAGATGT AGTGGATTTG 
GCAGCTAATT CACTCTTAAA CAAGTTAATC CATCAATCCT TAGTAGAATC CAGTCACCGT 
GTGGAAGTTT TACAGAAGGA TCCCAGCTCT CCACTTTACT CAGTAAAGAC ATTTGAAGAG 
CTGCGGCTAA AGGAAGAGTT ACTAAAAGGA ATCTATGCAA TGGGATTTAA TAGGCCATCT 
AAAATCCAAG AGATGGCTCT CCCTATGATG CTGGCACATC CACCCCAGAA CCTCATAGCA 
CAGAGCCAGT CTGGAACAGG AAAGACAGCG GCATTTGTGT TGGCAATGTT AAGCAGAGTT 
AATGCCTTGG AATTGTTCCC ACAGTGCCTC TGCCTAGCTC CTACTTATGA ATTGGCTCTG 
CAAACTGGCC GTGTGGTTGA GCAGATGGGA AAATTCTGTG TGGATGTTCA AGTGATGTAT 
GCCATTCGAG GGAATCGAAT TCCCAGAGGC ACCGACATCA CTAAACAGAT TATAATTGGC 
ACTCCTGGGA CTGTCCTAGA TTGGTGTTTT AAACTAAAAT TGATTGATTT GACTAAGATT 
CGTGTGTTTG TCCTGGATGA AGCAGATGTG ATGATTGACA CTCAAGGATT CTCAGATCAT 
AGTATTCGTA TTCAAAGAGC TCTACCCTCC GAATGCCAAA TGCTCCTCTT TTCAGCAACC 
TTTGAGGACT CTGTGTGGCA CTTTGCTGAG CGAATCATCC CTGACCCTAA TGTTATCAAG 
TTACGCAAAG AGGAGCTCAC ACTGAACAAC ATCCGGCAAT ATTACGTGCT GTGTGAGCAC 
AGGAAAGACA AATACCAAGC TCTGTGCAAC ATTTATGGCA GCATCACCAT TGGTCAGGCC 
ATCATCTTCT GCCAGACTCG TCGAAACGCT AAGTGGTTGA CCGTGGAGAT GATACAGGAT 
GGCCACCAGG TGTCTTTGTT AAGCGGGGAG CTGACCGTGG AGCAGCGAGC TTCCATCATT 
CAGAGGTTTC GGGATGGGAA AGAGAAGGTT CTCATAACAA CTAATGTTTG TGCCCGAGGG 
ATTGATGTGA AGCAGGTCAC AATTGTTGTG AACTTTGATC TCCCTGTAAA ACAAGGAGAG 
GAGCCGGACT ATGAGACCTA CCTCCACCGC ATAGGGCGGA CGGGGCGCTT TGGGAAAAAA 
GGCCTTGCCT TCAACATGAT TGAAGTAGAT GAGCTGCCCT CGCTCATGAA AATCCAGGAC 
CACTTTAACA GCAGTATTAA GCAACTCAAC GCTGAAGACA TGGATGAAAT TGAAAAGATT 
GACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( NM_001330438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317367.1 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 2, mRNA.

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CloneID	OHu102184
Clone ID Related Accession (Same CDS sequence)	NM_001330438.2 , NM_001330438.1
Accession Version	NM_001330438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000842.6, AL520666.3 and AL534333.3. On May 31, 2019 this sequence version replaced NM_001330438.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.260866.1, SRR3476690.727313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGATTTA ATAGGCCATC TAAAATCCAA GAGATGGCTC TCCCTATGAT GCTGGCACAT 
CCACCCCAGA ACCTCATAGC ACAGAGCCAG TCTGGAACAG GAAAGACAGC GGCATTTGTG 
TTGGCAATGT TAAGCAGAGT TAATGCCTTG GAATTGTTCC CACAGTGCCT CTGCCTAGCT 
CCTACTTATG AATTGGCTCT GCAAACTGGC CGTGTGGTTG AGCAGATGGG AAAATTCTGT 
GTGGATGTTC AAGTGATGTA TGCCATTCGA GGGAATCGAA TTCCCAGAGG CACCGACATC 
ACTAAACAGA TTATAATTGG CACTCCTGGG ACTGTCCTAG ATTGGTGTTT TAAACTAAAA 
TTGATTGATT TGACTAAGAT TCGTGTGTTT GTCCTGGATG AAGCAGATGT GATGATTGAC 
ACTCAAGGAT TCTCAGATCA TAGTATTCGT ATTCAAAGAG CTCTACCCTC CGAATGCCAA 
ATGCTCCTCT TTTCAGCAAC CTTTGAGGAC TCTGTGTGGC ACTTTGCTGA GCGAATCATC 
CCTGACCCTA ATGTTATCAA GTTACGCAAA GAGGAGCTCA CACTGAACAA CATCCGGCAA 
TATTACGTGC TGTGTGAGCA CAGGAAAGAC AAATACCAAG CTCTGTGCAA CATTTATGGC 
AGCATCACCA TTGGTCAGGC CATCATCTTC TGCCAGACTC GTCGAAACGC TAAGTGGTTG 
ACCGTGGAGA TGATACAGGA TGGCCACCAG GTGTCTTTGT TAAGCGGGGA GCTGACCGTG 
GAGCAGCGAG CTTCCATCAT TCAGAGGTTT CGGGATGGGA AAGAGAAGGT TCTCATAACA 
ACTAATGTTT GTGCCCGAGG GATTGATGTG AAGCAGGTCA CAATTGTTGT GAACTTTGAT 
CTCCCTGTAA AACAAGGAGA GGAGCCGGAC TATGAGACCT ACCTCCACCG CATAGGGCGG 
ACGGGGCGCT TTGGGAAAAA AGGCCTTGCC TTCAACATGA TTGAAGTAGA TGAGCTGCCC 
TCGCTCATGA AAATCCAGGA CCACTTTAAC AGCAGTATTA AGCAACTCAA CGCTGAAGAC 
ATGGATGAAA TTGAAAAGAT TGACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317367.1
CDS	383..1492
Translation	MGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	NM_001330438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330438.2

ORF Insert Sequence:

ATGGGATTTA ATAGGCCATC TAAAATCCAA GAGATGGCTC TCCCTATGAT GCTGGCACAT 
CCACCCCAGA ACCTCATAGC ACAGAGCCAG TCTGGAACAG GAAAGACAGC GGCATTTGTG 
TTGGCAATGT TAAGCAGAGT TAATGCCTTG GAATTGTTCC CACAGTGCCT CTGCCTAGCT 
CCTACTTATG AATTGGCTCT GCAAACTGGC CGTGTGGTTG AGCAGATGGG AAAATTCTGT 
GTGGATGTTC AAGTGATGTA TGCCATTCGA GGGAATCGAA TTCCCAGAGG CACCGACATC 
ACTAAACAGA TTATAATTGG CACTCCTGGG ACTGTCCTAG ATTGGTGTTT TAAACTAAAA 
TTGATTGATT TGACTAAGAT TCGTGTGTTT GTCCTGGATG AAGCAGATGT GATGATTGAC 
ACTCAAGGAT TCTCAGATCA TAGTATTCGT ATTCAAAGAG CTCTACCCTC CGAATGCCAA 
ATGCTCCTCT TTTCAGCAAC CTTTGAGGAC TCTGTGTGGC ACTTTGCTGA GCGAATCATC 
CCTGACCCTA ATGTTATCAA GTTACGCAAA GAGGAGCTCA CACTGAACAA CATCCGGCAA 
TATTACGTGC TGTGTGAGCA CAGGAAAGAC AAATACCAAG CTCTGTGCAA CATTTATGGC 
AGCATCACCA TTGGTCAGGC CATCATCTTC TGCCAGACTC GTCGAAACGC TAAGTGGTTG 
ACCGTGGAGA TGATACAGGA TGGCCACCAG GTGTCTTTGT TAAGCGGGGA GCTGACCGTG 
GAGCAGCGAG CTTCCATCAT TCAGAGGTTT CGGGATGGGA AAGAGAAGGT TCTCATAACA 
ACTAATGTTT GTGCCCGAGG GATTGATGTG AAGCAGGTCA CAATTGTTGT GAACTTTGAT 
CTCCCTGTAA AACAAGGAGA GGAGCCGGAC TATGAGACCT ACCTCCACCG CATAGGGCGG 
ACGGGGCGCT TTGGGAAAAA AGGCCTTGCC TTCAACATGA TTGAAGTAGA TGAGCTGCCC 
TCGCTCATGA AAATCCAGGA CCACTTTAAC AGCAGTATTA AGCAACTCAA CGCTGAAGAC 
ATGGATGAAA TTGAAAAGAT TGACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX25 ( NM_001330438.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317367.1 Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu102184
Clone ID Related Accession (Same CDS sequence)	NM_001330438.2 , NM_001330438.1
Accession Version	NM_001330438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-08
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX25 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000842.6, AL520666.3 and AL534333.3. On Aug 29, 2016 this sequence version replaced XM_017017626.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.260866.1, SRR1660805.187937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGATTTA ATAGGCCATC TAAAATCCAA GAGATGGCTC TCCCTATGAT GCTGGCACAT 
CCACCCCAGA ACCTCATAGC ACAGAGCCAG TCTGGAACAG GAAAGACAGC GGCATTTGTG 
TTGGCAATGT TAAGCAGAGT TAATGCCTTG GAATTGTTCC CACAGTGCCT CTGCCTAGCT 
CCTACTTATG AATTGGCTCT GCAAACTGGC CGTGTGGTTG AGCAGATGGG AAAATTCTGT 
GTGGATGTTC AAGTGATGTA TGCCATTCGA GGGAATCGAA TTCCCAGAGG CACCGACATC 
ACTAAACAGA TTATAATTGG CACTCCTGGG ACTGTCCTAG ATTGGTGTTT TAAACTAAAA 
TTGATTGATT TGACTAAGAT TCGTGTGTTT GTCCTGGATG AAGCAGATGT GATGATTGAC 
ACTCAAGGAT TCTCAGATCA TAGTATTCGT ATTCAAAGAG CTCTACCCTC CGAATGCCAA 
ATGCTCCTCT TTTCAGCAAC CTTTGAGGAC TCTGTGTGGC ACTTTGCTGA GCGAATCATC 
CCTGACCCTA ATGTTATCAA GTTACGCAAA GAGGAGCTCA CACTGAACAA CATCCGGCAA 
TATTACGTGC TGTGTGAGCA CAGGAAAGAC AAATACCAAG CTCTGTGCAA CATTTATGGC 
AGCATCACCA TTGGTCAGGC CATCATCTTC TGCCAGACTC GTCGAAACGC TAAGTGGTTG 
ACCGTGGAGA TGATACAGGA TGGCCACCAG GTGTCTTTGT TAAGCGGGGA GCTGACCGTG 
GAGCAGCGAG CTTCCATCAT TCAGAGGTTT CGGGATGGGA AAGAGAAGGT TCTCATAACA 
ACTAATGTTT GTGCCCGAGG GATTGATGTG AAGCAGGTCA CAATTGTTGT GAACTTTGAT 
CTCCCTGTAA AACAAGGAGA GGAGCCGGAC TATGAGACCT ACCTCCACCG CATAGGGCGG 
ACGGGGCGCT TTGGGAAAAA AGGCCTTGCC TTCAACATGA TTGAAGTAGA TGAGCTGCCC 
TCGCTCATGA AAATCCAGGA CCACTTTAAC AGCAGTATTA AGCAACTCAA CGCTGAAGAC 
ATGGATGAAA TTGAAAAGAT TGACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317367.1
CDS	335..1444
Translation	MGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQIIIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQMLLFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYGSITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY

Target ORF information:

RefSeq Version	NM_001330438.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330438.1

ORF Insert Sequence:

ATGGGATTTA ATAGGCCATC TAAAATCCAA GAGATGGCTC TCCCTATGAT GCTGGCACAT 
CCACCCCAGA ACCTCATAGC ACAGAGCCAG TCTGGAACAG GAAAGACAGC GGCATTTGTG 
TTGGCAATGT TAAGCAGAGT TAATGCCTTG GAATTGTTCC CACAGTGCCT CTGCCTAGCT 
CCTACTTATG AATTGGCTCT GCAAACTGGC CGTGTGGTTG AGCAGATGGG AAAATTCTGT 
GTGGATGTTC AAGTGATGTA TGCCATTCGA GGGAATCGAA TTCCCAGAGG CACCGACATC 
ACTAAACAGA TTATAATTGG CACTCCTGGG ACTGTCCTAG ATTGGTGTTT TAAACTAAAA 
TTGATTGATT TGACTAAGAT TCGTGTGTTT GTCCTGGATG AAGCAGATGT GATGATTGAC 
ACTCAAGGAT TCTCAGATCA TAGTATTCGT ATTCAAAGAG CTCTACCCTC CGAATGCCAA 
ATGCTCCTCT TTTCAGCAAC CTTTGAGGAC TCTGTGTGGC ACTTTGCTGA GCGAATCATC 
CCTGACCCTA ATGTTATCAA GTTACGCAAA GAGGAGCTCA CACTGAACAA CATCCGGCAA 
TATTACGTGC TGTGTGAGCA CAGGAAAGAC AAATACCAAG CTCTGTGCAA CATTTATGGC 
AGCATCACCA TTGGTCAGGC CATCATCTTC TGCCAGACTC GTCGAAACGC TAAGTGGTTG 
ACCGTGGAGA TGATACAGGA TGGCCACCAG GTGTCTTTGT TAAGCGGGGA GCTGACCGTG 
GAGCAGCGAG CTTCCATCAT TCAGAGGTTT CGGGATGGGA AAGAGAAGGT TCTCATAACA 
ACTAATGTTT GTGCCCGAGG GATTGATGTG AAGCAGGTCA CAATTGTTGT GAACTTTGAT 
CTCCCTGTAA AACAAGGAGA GGAGCCGGAC TATGAGACCT ACCTCCACCG CATAGGGCGG 
ACGGGGCGCT TTGGGAAAAA AGGCCTTGCC TTCAACATGA TTGAAGTAGA TGAGCTGCCC 
TCGCTCATGA AAATCCAGGA CCACTTTAAC AGCAGTATTA AGCAACTCAA CGCTGAAGAC 
ATGGATGAAA TTGAAAAGAT TGACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of the gonadotrophin-regulated testicular RNA helicase gene polymorphism with human male infertility.
Andrologia46(9)1063-6(2014)
Jaiswal D,Trivedi S,Agrawal NK,Singh K

Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent.
Human reproduction (Oxford, England)25(6)1383-97(2010 Jun)
Aston KI,Krausz C,Laface I,Ruiz-Castan? E,Carrell DT

Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis.
Journal of andrology31(1)45-52(2010 Jan-Feb)
Tsai-Morris CH,Sheng Y,Gutti RK,Tang PZ,Dufau ML

Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array.
British journal of cancer101(2)357-62(2009 Jul)
Kweekel DM,Antonini NF,Nortier JW,Punt CJ,Gelderblom H,Guchelaar HJ

Gonadotropin-regulated testicular helicase (GRTH/DDX25): an essential regulator of spermatogenesis.
Trends in endocrinology and metabolism: TEM18(8)314-20(2007 Oct)
Dufau ML,Tsai-Morris CH

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DDX25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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