Pld6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pld6
Entrez Gene ID	194908
Full Name	phospholipase D family, member 6
Synonyms	4933433K01Rik,Gm10,Zuc,mZuc,mitoPLD
General protein information	Preferred Names phospholipase D family, member 6 Names mitochondrial cardiolipin hydrolase choline phosphatase 6 mitochondrial phospholipase novel Phospholipase D Active site motif-containing protein phosphatidylcholine-hydrolyzing phospholipase D6 phospholipase D6 protein zucchini homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_183139.2	NP_898962.2	mitochondrial cardiolipin hydrolase isoform b
NM_001290283.1	NP_001277212.1	mitochondrial cardiolipin hydrolase isoform a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Phospholipase D Family Member 6 Is a Surface Marker for Enrichment of Undifferentiated Spermatogonia in Prepubertal Boars.
Zhang P, Qin Y, Zheng Y, Zeng W
Stem cells and development27(1)55-64(2018 Jan)

Switching of dominant retrotransposon silencing strategies from posttranscriptional to transcriptional mechanisms during male germ-cell development in mice.
Inoue K, Ichiyanagi K, Fukuda K, Glinka M, Sasaki H
PLoS genetics13(7)e1006926(2017 Jul)

Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.
Kabayama Y, Toh H, Katanaya A, Sakurai T, Chuma S, Kuramochi-Miyagawa S, Saga Y, Nakano T, Sasaki H
Nucleic acids research45(9)5387-5398(2017 May)

Coincident Phosphatidic Acid Interaction Restrains Drp1 in Mitochondrial Division.
Adachi Y, Itoh K, Yamada T, Cerveny KL, Suzuki TL, Macdonald P, Frohman MA, Ramachandran R, Iijima M, Sesaki H
Molecular cell63(6)1034-43(2016 Sep)

Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.
Zhang Y, Liu X, Bai J, Tian X, Zhao X, Liu W, Duan X, Shang W, Fan HY, Tong C
Molecular cell61(1)111-24(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Dnmt3l mutation greatly reduced DNA methylation levels at most retrotransposons, but its impact on their RNA abundance was limited in prospermatogonia. In Pld6 mutant germ cells, although only a few retrotransposons exhibited reduced DNA methylation, many showed increased expression at the RNA level.
Title: Switching of dominant retrotransposon silencing strategies from posttranscriptional to transcriptional mechanisms during male germ-cell development in mice.

MIWI (or PIWIL1) depletion had a smaller effect. In oocytes lacking PLD6 (or ZUCCHINI or MITOPLD), a mitochondrial nuclease/phospholipase involved in piRNA biogenesis in male germ cells, the piRNA level was decreased to 50% compared to wild-type.
Title: Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.

Drp1 binds a mitochondrial enzyme that produces phosphatidic acid, MitoPLD, and regulates mitochondrial division.
Title: Coincident Phosphatidic Acid Interaction Restrains Drp1 in Mitochondrial Division.

Propose that MIGA proteins promote mitochondrial fusion by regulating mitochondrial phospholipid metabolism via MitoPLD.
Title: Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.

crystal structure of mZuc at 1.75 A resolution indicates greater architectural similarity to phospholipase-D family nucleases than to phospholipases; data suggest that the Zucchini proteins act in primary piRNA biogenesis as nucleases, perhaps generating the 5' ends of primary piRNAs.
Title: The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis.

The following Pld6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pld6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17335	NM_001290283.1 Latest version!	Mus musculus phospholipase D family, member 6 (Pld6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu14250	NM_183139.2 Latest version!	Mus musculus phospholipase D family, member 6 (Pld6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pld6 ( NM_001290283.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277212.1 Mus musculus phospholipase D family, member 6 (Pld6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17335
Clone ID Related Accession (Same CDS sequence)	NM_001290283.1
Accession Version	NM_001290283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mitochondrial cardiolipin hydrolase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC119245.1, AL596204.8 and AW554531.2. On Mar 5, 2014 this sequence version replaced XM_006532599.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145052.1, CB272555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGCT CGAGTTGGCG GTTGGTGTTC GCGGCTGGTG CGGGTCTCGC GCTGGCCCTA 
GAGGCACTGC CGTGGCTGAT GCGTTGGCTG CTGGCTGGGC GGCGGCCAAG GCGCGAGGTG 
CTCTTCTTCC CCTCACAGGT GACCTGCACC GAGGCTTTAC TGCAGGCCCC AGGGTTGCCT 
CCCGGGCCCT CGGGCTGCCC GTGTAGCCTC CCCCACAGCG AGAGTTCACT GAGCCGCCTG 
CTGCGCGCGC TGTTGGCCGC CCGCTCCAGC TTGGAGCTCT GCCTCTTCGC CTTCTCCAGC 
CCGCAGCTGG GGCGTGCAGT GCAGCTGCTG CATCAGCGTG GGGTGCGCGT GCGGGTCATC 
ACTGACTGCG ACTACATGGC CCTCAACGGC TCTCAGATCG GCCTGCTGCG CAAGGCAGGG 
ATACAGGTAC GGCACGACCA GGACCTAGGC TACATGCACC ACAAGTTTGC CATCGTTGAC 
AAGAAGGTGC TCATCACCGG CTCCCTCAAC TGGACCACAC AGGCCATCCA GAACAACCGG 
GAGAACGTCC TGATTATGGA GGACACCGAG TATGTGCGGC TGTTCCTGGA GGAGTTTGAG 
CGCATCTGGG AAGAATTTGA CCCCACCAAG TACAGCTTTT TCCCCCAAAA GCATCGAGGG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277212.1
CDS	23..688
Translation	MGRSSWRLVFAAGAGLALALEALPWLMRWLLAGRRPRREVLFFPSQVTCTEALLQAPGLPPGPSGCPCSLPHSESSLSRLLRALLAARSSLELCLFAFSSPQLGRAVQLLHQRGVRVRVITDCDYMALNGSQIGLLRKAGIQVRHDQDLGYMHHKFAIVDKKVLITGSLNWTTQAIQNNRENVLIMEDTEYVRLFLEEFERIWEEFDPTKYSFFPQKHRGH

Target ORF information:

RefSeq Version	NM_001290283.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase D family, member 6 (Pld6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290283.1

ORF Insert Sequence:

ATGGGGCGCT CGAGTTGGCG GTTGGTGTTC GCGGCTGGTG CGGGTCTCGC GCTGGCCCTA 
GAGGCACTGC CGTGGCTGAT GCGTTGGCTG CTGGCTGGGC GGCGGCCAAG GCGCGAGGTG 
CTCTTCTTCC CCTCACAGGT GACCTGCACC GAGGCTTTAC TGCAGGCCCC AGGGTTGCCT 
CCCGGGCCCT CGGGCTGCCC GTGTAGCCTC CCCCACAGCG AGAGTTCACT GAGCCGCCTG 
CTGCGCGCGC TGTTGGCCGC CCGCTCCAGC TTGGAGCTCT GCCTCTTCGC CTTCTCCAGC 
CCGCAGCTGG GGCGTGCAGT GCAGCTGCTG CATCAGCGTG GGGTGCGCGT GCGGGTCATC 
ACTGACTGCG ACTACATGGC CCTCAACGGC TCTCAGATCG GCCTGCTGCG CAAGGCAGGG 
ATACAGGTAC GGCACGACCA GGACCTAGGC TACATGCACC ACAAGTTTGC CATCGTTGAC 
AAGAAGGTGC TCATCACCGG CTCCCTCAAC TGGACCACAC AGGCCATCCA GAACAACCGG 
GAGAACGTCC TGATTATGGA GGACACCGAG TATGTGCGGC TGTTCCTGGA GGAGTTTGAG 
CGCATCTGGG AAGAATTTGA CCCCACCAAG TACAGCTTTT TCCCCCAAAA GCATCGAGGG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld6 ( NM_183139.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_898962.2 Mus musculus phospholipase D family, member 6 (Pld6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14250
Clone ID Related Accession (Same CDS sequence)	NM_183139.2
Accession Version	NM_183139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mitochondrial cardiolipin hydrolase isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC119245.1, AL596204.8 and AW554531.2. On or before Feb 2, 2007 this sequence version replaced XM_110919.6, XM_988727.1, NM_183139.1. Transcript Variant: This variant (2) uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC119245.1, AK077214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGCT CGAGTTGGCG GTTGGTGTTC GCGGCTGGTG CGGGTCTCGC GCTGGCCCTA 
GAGGCACTGC CGTGGCTGAT GCGTTGGCTG CTGGCTGGGC GGCGGCCAAG GCGCGAGGTG 
CTCTTCTTCC CCTCACAGGT GACCTGCACC GAGGCTTTAC TGCAGGCCCC AGGGTTGCCT 
CCCGGGCCCT CGGGCTGCCC GTGTAGCCTC CCCCACAGCG AGAGTTCACT GAGCCGCCTG 
CTGCGCGCGC TGTTGGCCGC CCGCTCCAGC TTGGAGCTCT GCCTCTTCGC CTTCTCCAGC 
CCGCAGCTGG GGCGTGCAGT GCAGCTGCTG CATCAGCGTG GGGTGCGCGT GCGGGTCATC 
ACTGACTGCG ACTACATGGC CCTCAACGGC TCTCAGATCG GCCTGCTGCG CAAGGGATAC 
AGGTACGGCA CGACCAGGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_898962.2
CDS	23..466
Translation	MGRSSWRLVFAAGAGLALALEALPWLMRWLLAGRRPRREVLFFPSQVTCTEALLQAPGLPPGPSGCPCSLPHSESSLSRLLRALLAARSSLELCLFAFSSPQLGRAVQLLHQRGVRVRVITDCDYMALNGSQIGLLRKGYRYGTTRT

Target ORF information:

RefSeq Version	NM_183139.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase D family, member 6 (Pld6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183139.2

ORF Insert Sequence:

ATGGGGCGCT CGAGTTGGCG GTTGGTGTTC GCGGCTGGTG CGGGTCTCGC GCTGGCCCTA 
GAGGCACTGC CGTGGCTGAT GCGTTGGCTG CTGGCTGGGC GGCGGCCAAG GCGCGAGGTG 
CTCTTCTTCC CCTCACAGGT GACCTGCACC GAGGCTTTAC TGCAGGCCCC AGGGTTGCCT 
CCCGGGCCCT CGGGCTGCCC GTGTAGCCTC CCCCACAGCG AGAGTTCACT GAGCCGCCTG 
CTGCGCGCGC TGTTGGCCGC CCGCTCCAGC TTGGAGCTCT GCCTCTTCGC CTTCTCCAGC 
CCGCAGCTGG GGCGTGCAGT GCAGCTGCTG CATCAGCGTG GGGTGCGCGT GCGGGTCATC 
ACTGACTGCG ACTACATGGC CCTCAACGGC TCTCAGATCG GCCTGCTGCG CAAGGGATAC 
AGGTACGGCA CGACCAGGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phospholipase D Family Member 6 Is a Surface Marker for Enrichment of Undifferentiated Spermatogonia in Prepubertal Boars.
Stem cells and development27(1)55-64(2018 Jan)
Zhang P,Qin Y,Zheng Y,Zeng W

Switching of dominant retrotransposon silencing strategies from posttranscriptional to transcriptional mechanisms during male germ-cell development in mice.
PLoS genetics13(7)e1006926(2017 Jul)
Inoue K,Ichiyanagi K,Fukuda K,Glinka M,Sasaki H

Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.
Nucleic acids research45(9)5387-5398(2017 May)
Kabayama Y,Toh H,Katanaya A,Sakurai T,Chuma S,Kuramochi-Miyagawa S,Saga Y,Nakano T,Sasaki H

Coincident Phosphatidic Acid Interaction Restrains Drp1 in Mitochondrial Division.
Molecular cell63(6)1034-43(2016 Sep)
Adachi Y,Itoh K,Yamada T,Cerveny KL,Suzuki TL,Macdonald P,Frohman MA,Ramachandran R,Iijima M,Sesaki H

Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.
Molecular cell61(1)111-24(2016 Jan)
Zhang Y,Liu X,Bai J,Tian X,Zhao X,Liu W,Duan X,Shang W,Fan HY,Tong C

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Pld6 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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